KR920701460A - 생체내에서 해독후 변형부위를 포함하는 융합단백질과 그 융합 단백질의 제조 및 정제방법 - Google Patents

생체내에서 해독후 변형부위를 포함하는 융합단백질과 그 융합 단백질의 제조 및 정제방법

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Publication number
KR920701460A
KR920701460A KR1019910701555A KR910701555A KR920701460A KR 920701460 A KR920701460 A KR 920701460A KR 1019910701555 A KR1019910701555 A KR 1019910701555A KR 910701555 A KR910701555 A KR 910701555A KR 920701460 A KR920701460 A KR 920701460A
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KR
South Korea
Prior art keywords
fusion protein
dna sequence
protein
binding partner
amino acid
Prior art date
Application number
KR1019910701555A
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English (en)
Korean (ko)
Inventor
이. 크로안 쥬니어 존
Original Assignee
원본미기재
바이오테크놀러지 리서치 앤드 디벨로프먼트 코포레이숀
더 보드 오브 트러스티스 오브 더 유니버시티 오브 일리노이스
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Application filed by 원본미기재, 바이오테크놀러지 리서치 앤드 디벨로프먼트 코포레이숀, 더 보드 오브 트러스티스 오브 더 유니버시티 오브 일리노이스 filed Critical 원본미기재
Publication of KR920701460A publication Critical patent/KR920701460A/ko

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    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245—Escherichia (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
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    • C12N15/09—Recombinant DNA-technology
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    • C12N15/62—DNA sequences coding for fusion proteins
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
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    • C12N9/93—Ligases (6)
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    • C12Y102/00—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
    • C12Y102/04—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with a disulfide as acceptor (1.2.4)
    • C12Y102/04001—Pyruvate dehydrogenase (acetyl-transferring) (1.2.4.1)
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Y102/00—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
    • C12Y102/04—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with a disulfide as acceptor (1.2.4)
    • C12Y102/04002—Oxoglutarate dehydrogenase (succinyl-transferring) (1.2.4.2), i.e. alpha-ketoglutarat dehydrogenase
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    • C12Y102/00—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
    • C12Y102/05—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with a quinone or similar compound as acceptor (1.2.5)
    • C12Y102/05001—Pyruvate dehydrogenase (quinone) (1.2.5.1)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
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    • C07K2319/00—Fusion polypeptide
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    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/90—Fusion polypeptide containing a motif for post-translational modification

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  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
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  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
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  • Microbiology (AREA)
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  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Botany (AREA)
  • Cell Biology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
KR1019910701555A 1989-05-19 1990-05-17 생체내에서 해독후 변형부위를 포함하는 융합단백질과 그 융합 단백질의 제조 및 정제방법 KR920701460A (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US35426689A 1989-05-19 1989-05-19
US354266 1989-05-19
PCT/US1990/002852 WO1990014431A1 (fr) 1989-05-19 1990-05-17 Proteines de fusion ayant un site de modification post-translatif in vivo et procedes de fabrication et de purification

Publications (1)

Publication Number Publication Date
KR920701460A true KR920701460A (ko) 1992-08-11

Family

ID=23392544

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019910701555A KR920701460A (ko) 1989-05-19 1990-05-17 생체내에서 해독후 변형부위를 포함하는 융합단백질과 그 융합 단백질의 제조 및 정제방법

Country Status (6)

Country Link
EP (1) EP0472658A4 (fr)
JP (1) JPH04507341A (fr)
KR (1) KR920701460A (fr)
AU (1) AU647025B2 (fr)
CA (1) CA2057908A1 (fr)
WO (1) WO1990014431A1 (fr)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6072039A (en) * 1991-04-19 2000-06-06 Rohm And Haas Company Hybrid polypeptide comparing a biotinylated avidin binding polypeptide fused to a polypeptide of interest
US5395856A (en) * 1991-05-16 1995-03-07 Rohm And Haas Company HPLC avidin monomer affinity resin
US6107059A (en) * 1992-04-29 2000-08-22 Affymax Technologies N.V. Peptide library and screening method
DE69434520T3 (de) 1993-07-30 2009-10-15 Affymax, Inc., Palo Alto Biotinylierung von proteinen
FR2717499B1 (fr) * 1994-03-17 1996-05-24 Ulp Fragments d'anticorps recombinants synthétisés et biotinylés dans E. coli, leur utilisation en immunodosages et en purification par immunoaffinité.
WO1996021156A1 (fr) * 1995-01-04 1996-07-11 Abbott Laboratories Procede de preparation de cibles d'essai de proximite par scintillation
CU22559A1 (es) * 1996-01-17 1999-05-03 Ct Ingenieria Genetica Biotech Sistema de expresión de antígenos heterologos en e. coli como proteínas de fusión
US7166475B2 (en) * 1999-02-26 2007-01-23 Cyclacel Ltd. Compositions and methods for monitoring the modification state of a pair of polypeptides
GB0202018D0 (en) * 2002-01-29 2002-03-13 Sense Proteomic Ltd Tag and method
DE102006061002A1 (de) * 2006-12-22 2008-06-26 Profos Ag Verfahren und Mittel zur Anreicherung, Entfernung und zum Nachweis von gram-positiven Bakterien

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4782137A (en) * 1984-01-24 1988-11-01 Immunex Corporation Synthesis of protein with an identification peptide, and hybrid polypeptide incorporating same
WO1986002077A1 (fr) * 1984-10-02 1986-04-10 Meade Harry M Production de polypeptides analogues a la streptavidine
US4839293A (en) * 1986-02-24 1989-06-13 The Trustees Of Columbia University In The City Of New York DNA encoding streptavidin, streptavidin produced therefrom, fused polypeptides which include amino acid sequences present in streptavidin and uses thereof
DK105489D0 (da) * 1989-03-03 1989-03-03 Novo Nordisk As Polypeptid

Also Published As

Publication number Publication date
CA2057908A1 (fr) 1990-11-20
WO1990014431A1 (fr) 1990-11-29
EP0472658A1 (fr) 1992-03-04
AU647025B2 (en) 1994-03-17
AU5827090A (en) 1990-12-18
EP0472658A4 (en) 1992-08-26
JPH04507341A (ja) 1992-12-24

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