FR2717499B1 - Fragments d'anticorps recombinants synthétisés et biotinylés dans E. coli, leur utilisation en immunodosages et en purification par immunoaffinité. - Google Patents

Fragments d'anticorps recombinants synthétisés et biotinylés dans E. coli, leur utilisation en immunodosages et en purification par immunoaffinité.

Info

Publication number
FR2717499B1
FR2717499B1 FR9403125A FR9403125A FR2717499B1 FR 2717499 B1 FR2717499 B1 FR 2717499B1 FR 9403125 A FR9403125 A FR 9403125A FR 9403125 A FR9403125 A FR 9403125A FR 2717499 B1 FR2717499 B1 FR 2717499B1
Authority
FR
France
Prior art keywords
immunoaffinity
immunoassays
biotinylated
coli
fragments
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
FR9403125A
Other languages
English (en)
Other versions
FR2717499A1 (fr
Inventor
Etienne Weiss
Jean Chatellier
Georges Orfanoudakis
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
ULP
Original Assignee
ULP
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ULP filed Critical ULP
Priority to FR9403125A priority Critical patent/FR2717499B1/fr
Priority to AU19539/95A priority patent/AU1953995A/en
Priority to PCT/FR1995/000293 priority patent/WO1995025172A1/fr
Publication of FR2717499A1 publication Critical patent/FR2717499A1/fr
Application granted granted Critical
Publication of FR2717499B1 publication Critical patent/FR2717499B1/fr
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/24—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against cytokines, lymphokines or interferons
    • C07K16/241—Tumor Necrosis Factors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/40—Immunoglobulins specific features characterized by post-translational modification
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/55—Fab or Fab'
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/90—Fusion polypeptide containing a motif for post-translational modification

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
FR9403125A 1994-03-17 1994-03-17 Fragments d'anticorps recombinants synthétisés et biotinylés dans E. coli, leur utilisation en immunodosages et en purification par immunoaffinité. Expired - Fee Related FR2717499B1 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
FR9403125A FR2717499B1 (fr) 1994-03-17 1994-03-17 Fragments d'anticorps recombinants synthétisés et biotinylés dans E. coli, leur utilisation en immunodosages et en purification par immunoaffinité.
AU19539/95A AU1953995A (en) 1994-03-17 1995-03-13 Recombinant antibody fragments which are synthesized and biotinylated in e. coli, their use in immunoassays and immunopurification techniques
PCT/FR1995/000293 WO1995025172A1 (fr) 1994-03-17 1995-03-13 Fragments d'anticorps recombinants synthetises et biotinyles dans e. coli, leur utilisation en immunodosages et en purification par immunoaffinite

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR9403125A FR2717499B1 (fr) 1994-03-17 1994-03-17 Fragments d'anticorps recombinants synthétisés et biotinylés dans E. coli, leur utilisation en immunodosages et en purification par immunoaffinité.

Publications (2)

Publication Number Publication Date
FR2717499A1 FR2717499A1 (fr) 1995-09-22
FR2717499B1 true FR2717499B1 (fr) 1996-05-24

Family

ID=9461144

Family Applications (1)

Application Number Title Priority Date Filing Date
FR9403125A Expired - Fee Related FR2717499B1 (fr) 1994-03-17 1994-03-17 Fragments d'anticorps recombinants synthétisés et biotinylés dans E. coli, leur utilisation en immunodosages et en purification par immunoaffinité.

Country Status (3)

Country Link
AU (1) AU1953995A (fr)
FR (1) FR2717499B1 (fr)
WO (1) WO1995025172A1 (fr)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996021156A1 (fr) * 1995-01-04 1996-07-11 Abbott Laboratories Procede de preparation de cibles d'essai de proximite par scintillation
JP3942431B2 (ja) * 1999-08-31 2007-07-11 三菱化学株式会社 タンパク質−分子間相互作用解析法
DE50015156D1 (de) 1999-12-22 2008-06-26 Dade Behring Marburg Gmbh Gegen Procalcitonin gerichtete Antikörper, ihre Herstellung und Verwendung
DE50014159D1 (de) 1999-12-22 2007-04-26 Dade Behring Marburg Gmbh Lösungen von humanem Procalcitonin
FR2816943B1 (fr) * 2000-11-22 2004-02-13 Inst Nat Sante Rech Med Derives de la biotine, leurs procedes de fabrication et leurs utilisations en tant que vecteurs
DE10064827A1 (de) 2000-12-22 2002-06-27 Dade Behring Marburg Gmbh Nachweisverfahren
GB0202018D0 (en) 2002-01-29 2002-03-13 Sense Proteomic Ltd Tag and method
JP2007512543A (ja) 2003-12-01 2007-05-17 デイド・ベーリング・マルブルク・ゲゼルシヤフト・ミツト・ベシユレンクテル・ハフツング ホモジニアスな検出方法
DE102005026163A1 (de) 2004-08-24 2006-03-02 Dade Behring Marburg Gmbh Gegen den Marburg I Polymorphismus der Faktor VII aktivierenden Protease (FSAP) gerichtete Antikörper, ihre Herstellung und Verwendung
DE102005022047A1 (de) 2005-05-09 2006-11-30 Dade Behring Marburg Gmbh Bindungspartner des Plazentalen Wachstumsfaktors insbesondere gegen den Plazentalen Wachstumsfaktor gerichtete Antikörper, ihre Herstellung und Verwendung

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH04507341A (ja) * 1989-05-19 1992-12-24 バイオテクノロジー　リサーチ　アンド　ディヴェロプメント　コーポレーション ｉｎ　ｖｉｖｏ翻訳後修飾部位を有する融合タンパク質及び製造及び精製方法

Also Published As

Publication number Publication date
WO1995025172A1 (fr) 1995-09-21
FR2717499A1 (fr) 1995-09-22
AU1953995A (en) 1995-10-03

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Legal Events

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