KR920019929A - 미생물 살충제제의 제조방법 - Google Patents

미생물 살충제제의 제조방법 Download PDF

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Publication number
KR920019929A
KR920019929A KR1019920002839A KR920002839A KR920019929A KR 920019929 A KR920019929 A KR 920019929A KR 1019920002839 A KR1019920002839 A KR 1019920002839A KR 920002839 A KR920002839 A KR 920002839A KR 920019929 A KR920019929 A KR 920019929A
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South Korea
Prior art keywords
toxin protein
powder
silicon dioxide
dioxide powder
culture solution
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KR1019920002839A
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English (en)
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KR0186032B1 (ko
Inventor
다까야스 소노야마
시게오 야기
코우지 우찌야마
야스오 기다가끼
Original Assignee
요시도시 가즈오
시오노기 세이야구 가부시기가이샤
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Publication of KR920019929A publication Critical patent/KR920019929A/ko
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Publication of KR0186032B1 publication Critical patent/KR0186032B1/ko

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/02—Separating microorganisms from their culture media
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/832—Bacillus

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Botany (AREA)
  • Mycology (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

내용 없음

Description

미생물 살충제제의 제조방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음

Claims (5)

  1. 바실러스 투린기엔시스를 배지 중에서 배양하고, 배양액의 독소 단백을 침강시켜, 그 침강된 독소 단백을 함유하는 분리 하층액을 건조하는 것을 포함하는 미생물 살충제제의 제조방법에 있어서, 다음의 (a), (b) 그리고 (c)의 공정 중에서 적어도 하나의 공정을 포함하는 제조방법, (a) 질소원의 적어도 일종으로서 돈육 분말을 함유하는 배지 중에서 배양을 행하는 공정, (b) 그 균의 배양 종료 후의 배양액의 pH를 2~4로 조정하고, 독소 단백을 침강시키는 공정, 그리고 (c) 그 침강 독소 단백을 함유하는 배양액 하층을 분취하고, 이것에 이산화규소 분말을 첨가하여 얻어지는 혼합물로 분무 건조하는 공정.
  2. 제 1항에 있어서, 돈육 분말이 배지 성분의 총중량의 2~10중량%의 비율로 함유되는 제조방법.
  3. 제 1항에 있어서, 이산화규소 분말의 평균 입경이 6-8㎛인 제조방법.
  4. 제 1항에 있어서, 이산화규소 분말이 침강 독소 단백 함유액의 2~6중량%의 비율로 첨가되는 제조방법.
  5. 제 1항에 있어서, (a), (b) 그리고 (c)의 공정의 모든 것을 함유하는 제조방법.
    표 5의 결과로부터 이산화규소 분말인 카프렉스 #80을 사용하는 것에 의해 유동성이 개선되어, 분말의 회수율이 증가하는 것을 알 수 있다.
    본 발명에 의하면, 바실러스 투린기엔시스의 독소 단백을 함유하는 살충제제의 제조에 있어서, 균체의 증식이 촉진되어, 또한 독소 단백의 생산량이 높아지고, 그래서 번잡한 조작을 행하는 일이 없이 배양액을 처리하여 독소단백 함유액을 단시간에 건조시키는 것이 가능하게 된다. 이와 같이 미생물 살충제제가 효과적으로 제조되기 때문에 그 제제를 염가로 제공하는 것이 가능하게 된다.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019920002839A 1991-04-23 1992-02-25 미생물 살충제제의 제조방법 KR0186032B1 (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP91-091897 1991-04-23
JP3091897A JP2940885B2 (ja) 1991-04-23 1991-04-23 微生物殺虫製剤の製造方法

Publications (2)

Publication Number Publication Date
KR920019929A true KR920019929A (ko) 1992-11-20
KR0186032B1 KR0186032B1 (ko) 1999-04-01

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ID=14039361

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019920002839A KR0186032B1 (ko) 1991-04-23 1992-02-25 미생물 살충제제의 제조방법

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JP (1) JP2940885B2 (ko)
KR (1) KR0186032B1 (ko)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2973662A1 (en) * 2015-01-16 2016-07-21 Valent Biosciences Llc Bacillus thuringiensis subsp. kurstaki and bacillus thuringiensis subsp. aizawai combination formulations

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Publication number Publication date
JP2940885B2 (ja) 1999-08-25
JPH04321612A (ja) 1992-11-11
KR0186032B1 (ko) 1999-04-01

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