KR920004580A - 개선된 2-단계 pcr법 - Google Patents

개선된 2-단계 pcr법 Download PDF

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Publication number
KR920004580A
KR920004580A KR1019910010343A KR910010343A KR920004580A KR 920004580 A KR920004580 A KR 920004580A KR 1019910010343 A KR1019910010343 A KR 1019910010343A KR 910010343 A KR910010343 A KR 910010343A KR 920004580 A KR920004580 A KR 920004580A
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South Korea
Prior art keywords
pcr
step pcr
improved
dna
primer
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KR1019910010343A
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English (en)
Inventor
히사나가 이가라시
유꼬 아오노
사또시 오리따
아끼히꼬 사또
Original Assignee
요시또시 가즈오
시오노기세이야꾸 가부시끼가이샤
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Application filed by 요시또시 가즈오, 시오노기세이야꾸 가부시끼가이샤 filed Critical 요시또시 가즈오
Publication of KR920004580A publication Critical patent/KR920004580A/ko

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/702—Specific hybridization probes for retroviruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/702—Specific hybridization probes for retroviruses
    • C12Q1/703—Viruses associated with AIDS

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Virology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • AIDS & HIV (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

내용 없음

Description

개선된 2－단계 PCR법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 본 발명의 2－단계 PCR에서 프라이머(primer)의 상대적 위치를 나타낸다.

Claims (4)

  1. PCR을 2회 수행하고 제1－단계PCR에서의 프라이머(primer)농도가 5내지 25nm의 범위임을 특징으로하는, 2－ 단계 PCR 법.
  2. 제1항에 있어서, 상기 프라이머의 농도가
    10내지 25nm의 범위임을 특징으로 하는, 2－단계 PCR 법.
  3. 제1항 또는 2항에 있어서, 주형 DNA가 HTLV－1 DNA, HTLV－2 DNA 또는 HIV－1 DNA임을 특징으로 하는, 2－단계 PCR 법.
  4. 제1항 내지 3항중 어느 한 항에 있어서, 상기 PCR이 네스티드(nested)－프라이머 PCR임을 특징으로 하는, 2－단계 PCR 법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019910010343A 1990-08-02 1991-06-21 개선된 2-단계 pcr법 KR920004580A (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2206290A JPH0491790A (ja) 1990-08-02 1990-08-02 ２段階ｐｃｒ法
JP90-206290 1990-08-02

Publications (1)

Publication Number Publication Date
KR920004580A true KR920004580A (ko) 1992-03-27

Family

ID=16520860

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019910010343A KR920004580A (ko) 1990-08-02 1991-06-21 개선된 2-단계 pcr법

Country Status (3)

Country Link
EP (1) EP0469610A1 (ko)
JP (1) JPH0491790A (ko)
KR (1) KR920004580A (ko)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6589734B1 (en) 1989-07-11 2003-07-08 Gen-Probe Incorporated Detection of HIV
US5856088A (en) * 1989-07-11 1999-01-05 Gen-Probe Incorporated Detection of human immunodeficiency virus type 1
FR2677039B1 (fr) * 1991-05-31 1994-09-09 Cis Bio Int Oligonucleotides, utilisables comme reactifs pour la detection in vitro des infections a retrovirus de type hiv et procede de detection de retrovirus de type hiv.
US6300058B1 (en) 1992-01-29 2001-10-09 Hitachi Chemical Research Center, Inc. Method for measuring messenger RNA
EP0675965A4 (en) * 1992-01-29 1999-01-20 Hitachi Chemical Co Ltd CARRIERS FOR IMMOBILIZING POLYNUCLEOTIDES.
ES2048652B1 (es) * 1992-06-05 1994-10-01 Inst De Salud Carlos Iii Procedimientos de amplificacion de genoma para la deteccion e identificacion de secuencias genomicas relacionadas.
US5580971A (en) * 1992-07-28 1996-12-03 Hitachi Chemical Company, Ltd. Fungal detection system based on rRNA probes
EP0652971A4 (en) * 1992-07-28 1998-12-02 Hitachi Chemical Co Ltd GENE DETECTION SYSTEM.
US5340728A (en) * 1992-12-09 1994-08-23 E. I. Du Pont De Nemours And Company Method for amplification of targeted segments of nucleic acid using nested polymerase chain reaction
AU686616B2 (en) 1993-03-26 1998-02-12 Gen-Probe Incorporated Detection of human immunodeficiency virus type 1
US5403707A (en) * 1993-05-14 1995-04-04 Eastman Kodak Company Diagnostic compositions, elements, methods and test kits for amplification and detection of retroviral DNA using primers having matched melting temperatures
US6174668B1 (en) * 1993-05-14 2001-01-16 Johnson & Johnson Clinical Diagnostics, Inc. Diagnostic compositions, elements, methods and test kits for amplification and detection of two or more target DNA's using primers having matched melting temperatures
US6709813B1 (en) 1993-05-14 2004-03-23 Ortho-Clinical Diagnostics, Inc. Diagnostic compositions, elements, methods and test kits for amplification and detection of human CMV DNA using primers having matched melting temperatures
WO1995003430A1 (en) * 1993-07-23 1995-02-02 Gen-Probe Incorporated Methods for enhancing nucleic acid amplification
WO1996039535A1 (en) * 1995-06-06 1996-12-12 Jan Vijg Method of and apparatus for diagnostic dna testing
US6103468A (en) * 1997-10-07 2000-08-15 Labatt Brewing Company Limited Rapid two-stage polymerase chain reaction method for detection of lactic acid bacteria in beer
US20020031777A1 (en) * 2000-09-08 2002-03-14 Linda Starr-Spires Ultra yield amplification reaction
JP4768155B2 (ja) * 2001-07-02 2011-09-07 独立行政法人理化学研究所 鋳型ｄｎａの製造方法及びそれを用いた無細胞タンパク質合成系によるタンパク質の製造方法
JP4441170B2 (ja) 2002-11-28 2010-03-31 独立行政法人理化学研究所 Ｓ１２リボソームタンパク質に変異を有する大腸菌細胞抽出液及びそれを用いる無細胞系によるタンパク質の製造方法
JP4999460B2 (ja) 2003-10-13 2012-08-15 ゲナコ・バイオメデイカル・プロダクツ・インコーポレイテツド 核酸のプライマーに基づく増幅のための方法及びキット
JP4868731B2 (ja) 2004-11-17 2012-02-01 独立行政法人理化学研究所 哺乳動物培養細胞由来の無細胞タンパク質合成システム
JP4787488B2 (ja) 2004-11-19 2011-10-05 独立行政法人理化学研究所 直鎖状鋳型ｄｎａを用いた無細胞タンパク質合成方法及びそのための細胞抽出液
EP2213747B1 (en) 2007-11-05 2021-06-30 Riken Method for producing membrane protein
DK2539472T3 (da) 2010-02-26 2015-08-24 Hennessy Lori Hurtig pcr til str genotypebestemmelse
WO2017022696A1 (ja) 2015-07-31 2017-02-09 国立研究開発法人理化学研究所 膜タンパク質の製造方法およびその利用

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU629845B2 (en) * 1988-08-30 1992-10-15 Abbott Laboratories Detection and amplification of target nucleic acid sequences
EP0478630B1 (fr) * 1989-06-12 1993-09-01 Cis Bio International Procede de detection de sequences specifiques d'acides nucleiques et ses applications

Also Published As

Publication number Publication date
JPH0491790A (ja) 1992-03-25
EP0469610A1 (en) 1992-02-05

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