KR910700343A - 바실러스 써린젠시스의 신규 균주 - Google Patents
바실러스 써린젠시스의 신규 균주 Download PDFInfo
- Publication number
- KR910700343A KR910700343A KR1019900701750A KR900701750A KR910700343A KR 910700343 A KR910700343 A KR 910700343A KR 1019900701750 A KR1019900701750 A KR 1019900701750A KR 900701750 A KR900701750 A KR 900701750A KR 910700343 A KR910700343 A KR 910700343A
- Authority
- KR
- South Korea
- Prior art keywords
- btpgsi387
- strain
- protoxins
- crystals
- specifically
- Prior art date
Links
- 241000193830 Bacillus <bacterium> Species 0.000 title 1
- 239000013078 crystal Substances 0.000 claims 7
- 239000000203 mixture Substances 0.000 claims 6
- 239000003053 toxin Substances 0.000 claims 6
- 231100000765 toxin Toxicity 0.000 claims 6
- 108700012359 toxins Proteins 0.000 claims 6
- 238000000034 method Methods 0.000 claims 3
- 241000255777 Lepidoptera Species 0.000 claims 2
- 150000001875 compounds Chemical class 0.000 claims 2
- 230000000361 pesticidal effect Effects 0.000 claims 2
- 241000218473 Agrotis Species 0.000 claims 1
- 241001070941 Castanea Species 0.000 claims 1
- 235000014036 Castanea Nutrition 0.000 claims 1
- 108020004414 DNA Proteins 0.000 claims 1
- 108091028043 Nucleic acid sequence Proteins 0.000 claims 1
- 241000721452 Pectinophora Species 0.000 claims 1
- 241001439020 Phthorimaea Species 0.000 claims 1
- 241000256248 Spodoptera Species 0.000 claims 1
- 241000607479 Yersinia pestis Species 0.000 claims 1
- 239000004480 active ingredient Substances 0.000 claims 1
- 239000013599 cloning vector Substances 0.000 claims 1
- 238000012258 culturing Methods 0.000 claims 1
- 230000029087 digestion Effects 0.000 claims 1
- 229940096118 ella Drugs 0.000 claims 1
- 239000000575 pesticide Substances 0.000 claims 1
- 108090000623 proteins and genes Proteins 0.000 claims 1
- 102000004169 proteins and genes Human genes 0.000 claims 1
- 241000894007 species Species 0.000 claims 1
- 230000001131 transforming effect Effects 0.000 claims 1
- OOLLAFOLCSJHRE-ZHAKMVSLSA-N ulipristal acetate Chemical compound C1=CC(N(C)C)=CC=C1[C@@H]1C2=C3CCC(=O)C=C3CC[C@H]2[C@H](CC[C@]2(OC(C)=O)C(C)=O)[C@]2(C)C1 OOLLAFOLCSJHRE-ZHAKMVSLSA-N 0.000 claims 1
- 101710151559 Crystal protein Proteins 0.000 description 1
- 102000004142 Trypsin Human genes 0.000 description 1
- 108090000631 Trypsin Proteins 0.000 description 1
- 238000003119 immunoblot Methods 0.000 description 1
- 239000013612 plasmid Substances 0.000 description 1
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 1
- 239000012588 trypsin Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
- C07K14/325—Bacillus thuringiensis crystal peptides, i.e. delta-endotoxins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
- C12R2001/075—Bacillus thuringiensis
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- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Crystallography & Structural Chemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
내용 없음.
Description
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도 : 균주들〔1), Bt, HD-127, 2) BtPGSI387 및 3) BtS1으로부터 유래된 트립신 처리 결정 단백질의 SDS-PAGE 및 면역블롯트임.
제2도 : 그의 제한 부위를 나타내는 실시예 3의 p제37 플라스미드에 대한 개략도임.
Claims (8)
- BtPGSI387 균주, 구체적으로 정제된 형태의, 아주 구체적으로는 90.0 내지 99.9%의 순수한 세포로된 BtPGSI387 균주.
- BtPGSI387 결정 또는 제1 및 제2 BtPGSI387 프로톡신 또는 독소의 화합물, 구체적으로는 BtPGSI387 결정의 제1 및 제2 BtPGSI387 프로톡신물의 혼합물.
- BtPGSI387 균주, BtPGSI387 결정 또는 제1 및 제2 BtPGSI387 프로톡신 또는 독소의 혼합물로 되는 군중에서 선택된 유효성분으로 이루어지고, 특히 나비목에 대해 유효한 살충 조성물.
- 해충에 제3항에 따르는 살충제 조성물을 접촉시키는 것을 특징으로 하여, 모충, 특히 나비목, 구체적으로는 밤나방과, 명나방과 및 뿔나방과, 더욱 구체적으로는 스포돕테라(Spodoptera)종, 헬리오티스(Helithis)종, 아그로티스(Agrotis)종, 오스트리니아 누빌라리스(Ostrainia nubilalis)종, 디아트라애 그란디오셀라(Doatraea grandiosella), 프쏘리마애 오페르쿠렐라(Phthorimaea opcrculella) 및 펙티노포라 고씨엘라(Pcctinophora gossypiella)를 방제하는 방법.
- BtPGSI387 균주를 클로닝 벡터 중에 도입된 외부 DNA로 일레트로포레이션시키는 것을 특징으로 하여, BtPGSI387 균주를 Bt 프로톡신 또는 독소를 암호화하는 외부 DNA 서열로 형질전환시키는 방법..
- 제5항의 방법에 의해 제조된 형질전환된 BtPGSI387 균주.
- 제6항의 형질전환된 BtPGSI387 균주 또는 형질전환된 BtPGSI387 균주에 의해 생성된 결정 또는 결정 단백질로 되는 살충 조성물.
- BtPGSI387 균주를 배양시킨 다음, 임의로 BtPGSI387 결정을 분리 및 정제한 후, 임의로 BtPGSI387 결정으로부터 제1 및 제2 BtPGSI387 프로톡신화합물 또는 제1 및/또는 제2 BtPGSI387 프로톡신을 분리 및 정제하고, 이어서 임의로 제1 및/또는 제2 BtPGSI387 프로톡신을 트립신 분해시켜 제1 및/또는 제2 BtPGSI387 독소를 형성시키는 것을 특징으로 하여, BtPGSI387 결정 또는 제1 및 제2 BtPGSI387 프로톡신의 혼합물 또는 제2항의 독소 또는 제1 및/또는 제2 BtPGSI387 프로톡신 또는 톡신을 생성시키는 방법.※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP88403154 | 1988-12-12 | ||
| EP88403154.3 | 1988-12-12 | ||
| PCT/EP1989/001539 WO1990006999A1 (en) | 1988-12-12 | 1989-12-11 | New strain of bacillus thuringiensis |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| KR910700343A true KR910700343A (ko) | 1991-03-14 |
Family
ID=8200447
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1019900701750A KR910700343A (ko) | 1988-12-12 | 1989-12-11 | 바실러스 써린젠시스의 신규 균주 |
Country Status (5)
| Country | Link |
|---|---|
| EP (1) | EP0447493A1 (ko) |
| JP (1) | JPH05500151A (ko) |
| KR (1) | KR910700343A (ko) |
| AU (1) | AU635728B2 (ko) |
| WO (1) | WO1990006999A1 (ko) |
Families Citing this family (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3387495B2 (ja) * | 1990-04-26 | 2003-03-17 | プラント・ジエネテイツク・システムズ・エヌ・ベー | 新規のＢａｃｉｌｌｕｓ ｔｈｕｒｉｎｇｉｅｎｓｉｓ株及び殺虫毒素をコードするそれらの遺伝子 |
| US5885571A (en) | 1992-08-27 | 1999-03-23 | Plant Genetic Systems, N.V. | Bacillus thuringiensis strains and their insecticidal proteins |
| DE69325385T2 (de) * | 1992-11-24 | 2000-01-20 | Abbott Lab | Bacillus thuringiensis isolate die gegen lepidoptera-schädlinge aktiv sind |
| HU226143B1 (en) * | 1993-04-09 | 2008-05-28 | Plant Genetic Systems Nv | New bacillus thuringiensis strains and their insecticidal proteins |
| US6110668A (en) * | 1996-10-07 | 2000-08-29 | Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften E.V. | Gene synthesis method |
| EP0989998A1 (en) * | 1997-06-27 | 2000-04-05 | Aventis CropScience N.V. | Improved bacillus thuringiensis toxin |
| AU784649B2 (en) | 1999-12-28 | 2006-05-18 | Bayer Cropscience Nv | Insecticidal proteins from Bacillus thuringiensis |
| PT1988099E (pt) | 2001-01-09 | 2013-01-31 | Bayer Cropscience Nv | Proteínas inseticidas de bacillus thuringiensis |
| CA2911801A1 (en) | 2002-03-22 | 2003-10-02 | Greta Arnaut | Novel bacillus thuringiensis insecticidal proteins |
| US8173871B2 (en) | 2005-07-08 | 2012-05-08 | Universidad Nacional Autonoma De Mexico | Bacterial proteins with pesticidal activity |
| US9994621B2 (en) | 2007-06-01 | 2018-06-12 | Bayer Cropscience N.V. | Genes encoding insecticidal proteins |
| WO2009082208A2 (en) | 2007-12-21 | 2009-07-02 | Keygene N.V. | Trichome specific promoters |
| JP6215196B2 (ja) | 2011-05-31 | 2017-10-18 | キージーン・エン・フェー | 有害生物耐性植物 |
| EP2768955A1 (en) | 2011-10-19 | 2014-08-27 | Keygene N.V. | Methods and compositions for producing drimenol |
| US11236346B2 (en) | 2015-09-04 | 2022-02-01 | Keygene N.V. | Diplospory gene |
| BR112021023769A2 (pt) | 2019-05-29 | 2022-01-11 | Keygene Nv | Gene para partenogênese |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| BR8600161A (pt) * | 1985-01-18 | 1986-09-23 | Plant Genetic Systems Nv | Gene quimerico,vetores de plasmidio hibrido,intermediario,processo para controlar insetos em agricultura ou horticultura,composicao inseticida,processo para transformar celulas de plantas para expressar uma toxina de polipeptideo produzida por bacillus thuringiensis,planta,semente de planta,cultura de celulas e plasmidio |
| DE3685968T2 (de) * | 1985-01-22 | 1993-07-01 | Mycogen Corp | Zellulare einkapselung biologischer pestizide. |
| EP0325037B1 (en) * | 1987-12-24 | 1994-04-06 | Zeneca Limited | Bacterial strains |
| ES2099063T3 (es) * | 1988-05-20 | 1997-05-16 | Ciba Geigy Ag | Transformacion del bacillus thuringiensis. |
-
1989
- 1989-12-11 AU AU49490/90A patent/AU635728B2/en not_active Ceased
- 1989-12-11 JP JP2502317A patent/JPH05500151A/ja active Pending
- 1989-12-11 KR KR1019900701750A patent/KR910700343A/ko not_active Application Discontinuation
- 1989-12-11 WO PCT/EP1989/001539 patent/WO1990006999A1/en not_active Application Discontinuation
- 1989-12-11 EP EP90901767A patent/EP0447493A1/en not_active Withdrawn
Also Published As
| Publication number | Publication date |
|---|---|
| AU4949090A (en) | 1990-07-10 |
| EP0447493A1 (en) | 1991-09-25 |
| AU635728B2 (en) | 1993-04-01 |
| WO1990006999A1 (en) | 1990-06-28 |
| JPH05500151A (ja) | 1993-01-21 |
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| WITN | Application deemed withdrawn, e.g. because no request for examination was filed or no examination fee was paid |