KR910009276A - Extraction Method of Harpagoside-Containing Extract - Google Patents

Extraction Method of Harpagoside-Containing Extract Download PDF

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Publication number
KR910009276A
KR910009276A KR1019890016112A KR890016112A KR910009276A KR 910009276 A KR910009276 A KR 910009276A KR 1019890016112 A KR1019890016112 A KR 1019890016112A KR 890016112 A KR890016112 A KR 890016112A KR 910009276 A KR910009276 A KR 910009276A
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Prior art keywords
solvent
organic solvent
buffer solution
harpagoside
extract
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KR1019890016112A
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Korean (ko)
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KR920005686B1 (en
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최승용
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손성배
동국제약 주식회사
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/33—Cactaceae (Cactus family), e.g. pricklypear or Cereus

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  • Health & Medical Sciences (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Alternative & Traditional Medicine (AREA)
  • Biotechnology (AREA)
  • Botany (AREA)
  • Medical Informatics (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Medicines Containing Plant Substances (AREA)
  • Saccharide Compounds (AREA)

Abstract

내용 없음No content

Description

하르파고시드 함유 엑기스의 추출방법Extraction Method of Harpagoside-Containing Extract

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음As this is a public information case, the full text was not included.

제 1도는 본 발명에 따른 하르파고시드(Harpaposide) 엑기스의 추출장치이다.1 is an apparatus for extracting Harpaposide extract according to the present invention.

Claims (7)

(가)하르파고피텀 프로쿰벤스 D.C. (Harpapophytum Procumbens D.C)의 발톱부분을 잘게 분쇄하여 70℃의 온도가 가온된 pH7의 인산염 완충용 액으로 추출하고, (나) 이렇게 하여 추출된 완충용액을 직쇄상 탄화수소 계열의 용매로 환류추출하여 오일상물질을 제거해낸 다음, (다) 완충용액층을 회수하여 할로겐함유 탄화수소 계열의 유기용매로 색소성물질을 추출제거하고, (라) 다시 완충용액층을 회수하여 에틸아세테이트 용매로 정제하는 것을 특징으로 하는 하르파고시드 함유 엑기스의 추출방법.Harpagopitum Procumbens D.C. (Harpapophytum Procumbens DC) finely pulverized the toenail and extracted with a phosphate buffer solution of pH7 heated to 70 ℃ temperature, and (b) the buffer solution thus extracted to reflux with a linear hydrocarbon solvent After removing the daily substance, (C) recovers the buffer layer, extracts and removes the pigment material with a halogen-containing hydrocarbon-based organic solvent, and (D) recovers the buffer layer again and purifies with ethyl acetate solvent. Extraction method of harpagoside containing extract made into. 제 1항에 있어서, (가)공정의 분쇄된 하르파고피텀 프로쿰벤스 D.C는 인산염 완충용액으로 추출하기 이전에 80℃로 가온된 질소기체는 멸균 처리하는 것을 특징으로 하는 방법.The method of claim 1, wherein (a) The pulverized Harpagophytum procumbens D.C. process is characterized in that the nitrogen gas heated to 80 ℃ sterilization before extraction with phosphate buffer solution. 제 1항에 있어서, (나)공정의 직쇄상 탄화수소 계열의 용매로는 엔-핵산, 엔-햅탄, 석유에테를 사용함을 특징으로 하는 방법.The method of claim 1, wherein (b) N-nucleic acid, N-haptan, petroleum ether are used as the linear hydrocarbon solvent. 제 1항에 있어서, (다)공정으이 할로겐함유 탄화수소 계열의 유기용매로는 사염화탄소, 클로로포름, 디클로에탄올을 사용함을 특징으로 하는 방법.The method according to claim 1, wherein (c) the halogen-containing hydrocarbon-based organic solvent is carbon tetrachloride, chloroform, dichloroethanol. 제 1항에 있어서, (가)(나)(다)(라) 공정을 연속적으로 12회 반복실시하되, (라)공정에서 (가)공정으로 이동될때는 pH7의 완충용액으로 재조정 하는 것을 특징으로 하는 방법.The method of claim 1, wherein the steps (a) (b) (c) (d) are repeated 12 times in succession, and the method is readjusted with a buffer solution of pH 7 when it is moved from step (d) to step (a). How to. 제 1항에서 얻어진 하르파고시드 함유 엑기스를 농출 하여 물과 에틸아세테이트의 혼합용매로 추출하고, 수용액층을 분리하여 목탄 컬럼 크로마토그래피에서 메탄올로 용출시킨 다음, 이어서 실리카겔 컬럼크로마토그래피에서 메탄올:클로로포름=1:9의 혼합용매로 용출시키고, 그 용출액을 감압, 농축하는 것을 특징으로 하는 자사용 하르파고시드 엑기스의 제조방법.The Harpagoside-containing extract obtained in claim 1 was concentrated, extracted with a mixed solvent of water and ethyl acetate, the aqueous layer was separated, eluted with methanol in charcoal column chromatography, and then methanol: chloroform = in silica gel column chromatography. Elution with a 1: 9 mixed solvent, the eluate is concentrated under reduced pressure, characterized in that the method for producing a self-harpagoside extract. 질소주입관 및 배출관이 상하로 설치되어 있는 컬럼(1)과, 직쇄상 탄화수소 계열의 유기용매가 들어있는 제1유기용매실(3), 할로겐함유탄화수소계열의 유기용매가 들어 있는 제2유기용매실(4), 및 에틸아세테이트용매가 들어 있는 제3유기용매실(5), 그리고 완충용액저장조(7)가 서로 연결관을 따라 순환식으로 연결되어 있되, 상기 컬럼(1)과 제1유기용매실(3) 사이에는 압력조절용 밸브(8) 및 열교환기(2a)가 설치되어 있고, 완충용액저장조(7)와 컬럼(1) 사이에는 펌프(10)가 설치되어 있으며, 제3유기용매실(5)은 농축조(6)와 연결되어 있는 것을 특징으로 하는 하르파고시드 엑기스의 추출장치.A column (1) having a nitrogen injection pipe and a discharge pipe up and down, a first organic solvent chamber (3) containing a linear hydrocarbon-based organic solvent, and a second organic solvent containing a halogen-containing hydrocarbon series organic solvent. The plum (4), the third organic solvent chamber (5) containing the ethyl acetate solvent, and the buffer solution storage tank (7) are connected to each other in a circulating manner along the connecting pipe, the column (1) and the first organic A pressure regulating valve 8 and a heat exchanger 2a are provided between the solvent chambers 3, and a pump 10 is installed between the buffer solution tank 7 and the column 1, and the third organic Plum (5) is an extracting device of harpagoside extract, characterized in that connected to the concentration tank (6). ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019890016112A 1989-11-07 1989-11-07 Process for the preparation by extracting of harpagophytum KR920005686B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1019890016112A KR920005686B1 (en) 1989-11-07 1989-11-07 Process for the preparation by extracting of harpagophytum

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Application Number Priority Date Filing Date Title
KR1019890016112A KR920005686B1 (en) 1989-11-07 1989-11-07 Process for the preparation by extracting of harpagophytum

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KR910009276A true KR910009276A (en) 1991-06-28
KR920005686B1 KR920005686B1 (en) 1992-07-13

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100333784B1 (en) * 2000-02-15 2002-04-26 주재성 Colored waste fiber and fiber ball, and method for producting them
KR100420227B1 (en) * 2001-04-06 2004-03-03 제일방염 주식회사 Multiple functional coating material

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19780262D2 (en) * 1996-03-21 1999-03-11 Schwabe Willmar Gmbh & Co Harpagoside
AU714643B2 (en) * 1996-05-18 2000-01-06 Finzelberg Gmbh & Co. Kg. A purified extract from harpagophytum procumbens and/or harp agophytum zeyheri DENCE, a process for its preparation and its use
FR2915900B1 (en) 2007-05-07 2012-08-24 Burgundy NEW PROCESS FOR THE PREPARATION OF PURIFIED EXTRACTS OF HARPAGOPHYTUM PROCUMBENS.
CN107456528A (en) * 2017-08-10 2017-12-12 靖西市民族医药协会 A kind of medicinal liquor for treating rheumatic arthritis

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100333784B1 (en) * 2000-02-15 2002-04-26 주재성 Colored waste fiber and fiber ball, and method for producting them
KR100420227B1 (en) * 2001-04-06 2004-03-03 제일방염 주식회사 Multiple functional coating material

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