KR900700886A - 단백질의 방사선라벨링 방법 - Google Patents

단백질의 방사선라벨링 방법

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Publication number
KR900700886A
KR900700886A KR1019890702248A KR890702248A KR900700886A KR 900700886 A KR900700886 A KR 900700886A KR 1019890702248 A KR1019890702248 A KR 1019890702248A KR 890702248 A KR890702248 A KR 890702248A KR 900700886 A KR900700886 A KR 900700886A
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South Korea
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protein
antibody
fragment
fab
solution
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KR1019890702248A
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English (en)
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KR0151105B1 (ko
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쇼차트 단
제이. 한센 한스
순도로-우 로버트
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원본미기재
이뮤노메딕스 인코포레이티드
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/534—Production of labelled immunochemicals with radioactive label
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K51/00—Preparations containing radioactive substances for use in therapy or testing in vivo
    • A61K51/02—Preparations containing radioactive substances for use in therapy or testing in vivo characterised by the carrier, i.e. characterised by the agent or material covalently linked or complexing the radioactive nucleus
    • A61K51/04—Organic compounds
    • A61K51/08—Peptides, e.g. proteins, carriers being peptides, polyamino acids, proteins
    • A61K51/10—Antibodies or immunoglobulins; Fragments thereof, the carrier being an antibody, an immunoglobulin or a fragment thereof, e.g. a camelised human single domain antibody or the Fc fragment of an antibody
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K51/00—Preparations containing radioactive substances for use in therapy or testing in vivo
    • A61K51/02—Preparations containing radioactive substances for use in therapy or testing in vivo characterised by the carrier, i.e. characterised by the agent or material covalently linked or complexing the radioactive nucleus
    • A61K51/04—Organic compounds
    • A61K51/08—Peptides, e.g. proteins, carriers being peptides, polyamino acids, proteins
    • A61K51/10—Antibodies or immunoglobulins; Fragments thereof, the carrier being an antibody, an immunoglobulin or a fragment thereof, e.g. a camelised human single domain antibody or the Fc fragment of an antibody
    • A61K51/1045—Antibodies or immunoglobulins; Fragments thereof, the carrier being an antibody, an immunoglobulin or a fragment thereof, e.g. a camelised human single domain antibody or the Fc fragment of an antibody against animal or human tumor cells or tumor cell determinants
    • A61K51/1048—Antibodies or immunoglobulins; Fragments thereof, the carrier being an antibody, an immunoglobulin or a fragment thereof, e.g. a camelised human single domain antibody or the Fc fragment of an antibody against animal or human tumor cells or tumor cell determinants the tumor cell determinant being a carcino embryonic antigen
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2123/00—Preparations for testing in vivo
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/804—Radioisotope, e.g. radioimmunoassay

Abstract

내용없음

Description

단백질이 방서선라벨링 방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음

Claims (40)

  1. 적어도 하나의 펜던트 설프하이드릴 그룹을 함유하는 단백질 용액을 설프하이드릴 그룹과 단단하게 결합하는 방사선 핵종의 방사선 금속이온의 용액과 접촉시키고, 방사선라벨된 단백질의 생성된 용액을 회수하는 단계를 포함함을 특징으로 하는, 단백질의 직접 방사선라벨링 방법.
  2. 제1항에 있어서, 단백질 용액이 저분자량이 티올 화합물을 실질적으로 함유하지 않는 방법.
  3. 제1항에 있어서, 적어도 하나의 펜던트 설프하이드릴 그립을 함유하고, 또한 디설파이드 그룹을 환원시키고 설프하이드릴 그룹을 안정화시키기 위한 저분자량의 분자 또는 이온을 함유하는 단백질의 용액을 사이징(sizing) 컬럼에 통과시켜 저분자량의 분자 및 이온을 제거하고, 생성된 단백질 용액을 회수함으로써 단백질 용액을 제조하는 방법.
  4. 제1항에 있어서, 단백질이 항체 또는 항체 단편인 방법.
  5. 제4항에 있어서, 단백질이 좀더 고분자량의 항체 또는 항체 단편 전구체내의 디설파이드 결합을 환원시킴으로서 제조된 항체 단편인 방법.
  6. 제5항에 있어서, 항체 단편이 2가 F(ab)2또는 F(ab)2전구체 단편의 환원적 분해에 의해 제조된 1가 Fab-SH 또는 Fab`-SH단편인 방법.
  7. 제5항에 있어서, 항체 단편이 완전한 면역 글로불린의 환원적 분해에 의해 제조된 1가 Fab`c-SH 경/중쇄 단편인 방법.
  8. 제6항에 있어서, 방사선핵종이 Tc-99m인 방법.
  9. 제6항에 있어서, 방사선핵종이 Re-186인 방법.
  10. 제6항에 있어서, 방사선핵종이 Re-188, Cu-67, Hg-197, Pb-203 또는 Bi-212인 방법.
  11. 제8항에 있어서, 접촉반응을 환원된 과테크네튬염 이온에 대한 트랜스킬레이터의 존재하에 수행하는 방법.
  12. 제8항에 있어서, 방사선라벨된 생성 단백질을 방사선 금속이온과 단단히 결합하는 외인성 리간드와 접촉시키고, 안정화되고 방사선라벨된 생성 단백질을 회수하는 방법.
  13. 제1항에 있어서, 단백질 용액이 설프하이드릴 그룹을 환원시키거나 안정화시키기 위한 저분자량의 분자량이 분자 또는 이온을 함유하고, 언급된 용액을 설프하이드릴 그룹과 단단히 결합하는 방사선핵종의 방사선 금속이온의 용액이 적재된 분자 사이징 겔 컬럼에 적용시키며, 언급된 컬럼을 용출시킨 후, 저분자량의 분자 및 비결합된 방사선 금속이온이 실질적으로 함유되지 않은 생성된 용액을 회수하는 방법.
  14. 제13항에 있어서, 사이징 겔 컬럼이 추가로 방사선 금속이온에 대한 트랜스킬레이터를 함유하는 방법.
  15. 제13항에 있어서, 컬럼을 방사선 금속이온에 대한 트랜스킬레이터의 용액으로 용출시키는 방법.
  16. 제13항에 있어서, 단백질이 항체 또는 항체단편인 방법.
  17. 제16항에 있어서, 항체 또는 항체 단편이 부분적으로 환원된 완전한 면역글로블린, 부분적으로 환원된 F(ab`)2, 부분적으로 환원된 F(ab)2, Fab`-SH, Fab-SH 또는 Fabc`-SH인 방법.
  18. 제13항에 있어서, 방사선핵종이 Tc-99m인 방법.
  19. 제17항에 있어서, 방사선핵종이 Tc-99m인 방법.
  20. 제12항에 있어서, 외인성 리간드가 6-머캅토 퓨린 또는 2-아미노에탄티올인 방법.
  21. 제12항에 있어서, 외인성 리간드가 이를 저면역원성으로 되게 하는 당, 덱스트란 또는 포리올 잔기를 포함하는 방법.
  22. 제12항에 있어서, 방사선 핵종이 세포독성 표적된 치료를 위한 고에너지 β방사체 또는 α방사체인 방법.
  23. 제22항에 있어서, 외인성 리간드가 방사선증감제를 포함하는 방법.
  24. 셀프하이드릴 그룹과 단단히 결합하는 방사선핵종의 방사선 금속이온에 결합된 적어도 하나의 펜던트 설프하이드릴 그룹을 함유하며, 상기 방사선 금속이온 또한 설프하이드릴 그룹과 단단히 결합하는 외인성 리간드에 결합된 방사선금속-라벨된 단백질.
  25. 제24항에 있어서, 단백질이 항체 또는 항체단편인 방사선라벨된 단백질.
  26. 제25항에 있어서, 라벨된 Fab`또는 Fab 단편인 방사선라벨된 항체 또는 단편.
  27. 제25항에 있어서, 방사선 금속이 Tc-99m인 방사선 라벨된 항체 또는 단편.
  28. 제26항에 있어서, 방사선 금속이 Tc-99m인 방사선 라벨된 항체 단편.
  29. 제1항의 방법에 의해 라벨된 방사선 금속-라벨된 단백질.
  30. 제29항에 있어서, 단백질이 항체 또는 항체 단편인 방사선라벨된 단백질.
  31. 제30항에 있어서, 라벨된 Fab`또는 Fab 단편인 방사선라벨된 항체 또는 단편.
  32. 제30항에 있어서, 방사선 금속이 Tc-99m인 방사선 라벨된 항체 또는 단편.
  33. 제32항에 있어서, 방사선 금속이 Tc-99m인 방사선 라벨된 항체 단편.
  34. 적합한 용기내에 (a) 적어도 하나의 유리 설프하이드릴 그룹을 함유하는 단백질 및 (b) 괴테크네튬염을 환원시키기 위한 주석이온의 공급원을 함유함을 특징으로 하는, Tc-99m으로 단백질을 방사선라벨링시키기에 적합한 키트.
  35. 제34항에 있어서, 단백질이 항체 또는 항체 단편인 키트.
  36. 제34항에 있어서, 단백질이 Fab-SH 또는 Fab`-SH인 키트.
  37. 제36항에 있어서, Fab-SH 또는 Fab`-SH가 동결 건조된 키트.
  38. 제35항에 있어서, 주석이온의 공급원이 염화주석 또는 주석 글루코헵토네이트인 키트.
  39. 제34항에 있어서, 환원된 콰테크네튬염에 대한 트랜스킬레이터러를 추가로 함유하는 키트.
  40. 제14항에 있어서, 트랜스킬레이터가 겔에 결합된 방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019890702248A 1988-04-01 1989-03-29 단백질의 직접적 방사선 라벨링 방법 KR0151105B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US176,421 1988-04-01
US07/176,421 US5061641A (en) 1988-04-01 1988-04-01 Method for radiolabeling proteins
PCT/US1989/001217 WO1989009405A1 (en) 1988-04-01 1989-03-29 Method for radiolabeling proteins

Publications (2)

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KR900700886A true KR900700886A (ko) 1990-08-17
KR0151105B1 KR0151105B1 (ko) 1999-05-15

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US (2) US5061641A (ko)
EP (1) EP0336678B1 (ko)
JP (1) JP2521168B2 (ko)
KR (1) KR0151105B1 (ko)
AT (1) ATE130288T1 (ko)
AU (1) AU617826B2 (ko)
CA (1) CA1340167C (ko)
DE (1) DE68924795T2 (ko)
DK (1) DK235390A (ko)
ES (1) ES2078907T3 (ko)
FI (1) FI904788A0 (ko)
IE (1) IE70588B1 (ko)
IL (1) IL89795A (ko)
WO (1) WO1989009405A1 (ko)
ZA (1) ZA892342B (ko)

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DE68924795D1 (de) 1995-12-21
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IL89795A0 (en) 1989-09-28
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DE68924795T2 (de) 1996-05-30
US5061641A (en) 1991-10-29
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US5514363A (en) 1996-05-07
ZA892342B (en) 1989-12-27
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