KR900006512A - 방 법 - Google Patents
방 법 Download PDFInfo
- Publication number
- KR900006512A KR900006512A KR1019890015025A KR890015025A KR900006512A KR 900006512 A KR900006512 A KR 900006512A KR 1019890015025 A KR1019890015025 A KR 1019890015025A KR 890015025 A KR890015025 A KR 890015025A KR 900006512 A KR900006512 A KR 900006512A
- Authority
- KR
- South Korea
- Prior art keywords
- mixture
- reagent
- group
- contaminating protein
- pyridylthio
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/315—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Streptococcus (G), e.g. Enterococci
- C07K14/3153—Streptokinase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/814—Enzyme separation or purification
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/814—Enzyme separation or purification
- Y10S435/815—Enzyme separation or purification by sorption
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/8215—Microorganisms
- Y10S435/822—Microorganisms using bacteria or actinomycetales
- Y10S435/885—Streptococcus
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Gastroenterology & Hepatology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Enzymes And Modification Thereof (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Glass Compositions (AREA)
- Iron Core Of Rotating Electric Machines (AREA)
- Steroid Compounds (AREA)
- Removal Of Specific Substances (AREA)
Abstract
내용 없음
Description
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
Claims (10)
- 환원제로 혼합물을 처리하여 오염 단백질내 디설파이드 브릿지를 유리 티올기로 환원시키고, 시약 R-X(여기서, R은 유리 티올기와 반응시킬 수 있는 기이고, X는 유리 티올기와 반응시킬 수 있는 기 R'이거나 티올-함유 메트릭스이다)와 혼합물을 접촉시키고 난후, 결과 형성된 화학적으로 개질시킨 오염 단백질을 혼합물로 부터 분리시켜 오염 단백질이 실질적으로 없는 형태로 스트랩토키나아제를 분리시키는 방법.
- 제1항에 있어서, 환원제가 디티오트레이톨인 방법.
- 제1항 또는 제2항에 있어서, R 및 R'기가 5-니트로-2-피리딜티오 5-카르복시 -2-피리딜티오 2-피리딜티오 4-피리딜티오 2-벤조티아졸릴티오 4-니트로-3-카르복시페닐티오 및 상기 피리딜기의 N-산화물로 부터 선택되는 방법.
- 제1항 내지 제3항중 어느 한 항에 있어서, 시약 R-X가 R-R'형태인 방법.
- 제4항에 있어서, 시약 R-R'이 2-2'-디피리틸디설파이드인 방법.
- 제4항 또는 제5항에 있어서, 시약 R-R'의 농도가 10-200mM인 방법.
- 제2항 내지 제6항중 어느 한 항에 있어서, 시약 R-R'과 환원시킨 혼합물과의 반응이 pH 6.0-8.5에서 수행되는 방법.
- 제4항 내지 제7항중 어느 한 항에 있어서, 시약 R-R'과 환원시킨 혼합물과의 반응이 5-35℃에서 수행되는 방법.
- 제4항 내지 제8항중 어느 한 항에 있어서, 결과형성된 화학적으로 개질시킨 오염 단백질이 여과, 침강, 원심분리법에 의해 또는 크로마토그래피 컬럼 내체류에 의해 분리시킨 침전물을 형성시키는 방법.
- 제9항에 있어서, 화학적으로 개질시킨 잔류 오염 단백질이 티올 교환 크로마토그래피에 의해 제거되는 방법.※ 참고사항 최초출원 내용에 의하여 공개하는 것임.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB8824496.7 | 1988-10-19 | ||
GB888824496A GB8824496D0 (en) | 1988-10-19 | 1988-10-19 | Process |
Publications (2)
Publication Number | Publication Date |
---|---|
KR900006512A true KR900006512A (ko) | 1990-05-08 |
KR0136613B1 KR0136613B1 (ko) | 1998-04-25 |
Family
ID=10645453
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
KR1019890015025A KR0136613B1 (ko) | 1988-10-19 | 1989-10-18 | 스트렙토키나아제의 정제 방법 |
Country Status (15)
Country | Link |
---|---|
US (1) | US5334384A (ko) |
EP (1) | EP0365278B1 (ko) |
JP (1) | JP2837467B2 (ko) |
KR (1) | KR0136613B1 (ko) |
AT (1) | ATE103633T1 (ko) |
AU (1) | AU622492B2 (ko) |
CA (1) | CA2000850A1 (ko) |
DE (1) | DE68914242T2 (ko) |
DK (1) | DK515889A (ko) |
ES (1) | ES2062029T3 (ko) |
GB (1) | GB8824496D0 (ko) |
IE (1) | IE63478B1 (ko) |
NZ (1) | NZ231039A (ko) |
PT (1) | PT92024B (ko) |
ZA (1) | ZA897849B (ko) |
Families Citing this family (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1999040188A2 (en) | 1998-02-05 | 1999-08-12 | Smithkline Beecham Biologicals S.A. | Tumor-associated antigen derivatives from the mage family, and nucleic acid sequences encoding them, used for the preparation of fusion proteins and of compositions for vaccinations |
US6388073B1 (en) | 2000-07-26 | 2002-05-14 | Shire Us Inc. | Method for the manufacture of anagrelide |
US7094351B2 (en) | 2000-10-12 | 2006-08-22 | Corcoran Robert C | Purification of substances by reaction affinity chromatography |
KR20020007231A (ko) * | 2001-08-01 | 2002-01-26 | 이병철 | 스트렙토키나제의 정제방법 |
US7700608B2 (en) | 2004-08-04 | 2010-04-20 | Shire Holdings Ag | Quinazoline derivatives and their use in the treatment of thrombocythemia |
US20060030574A1 (en) * | 2004-08-04 | 2006-02-09 | Shire Holdings Ag | Quinazoline derivatives useful for the treatment of peripheral arterial disease and as phosphodiesterase inhibitors |
US8304420B2 (en) | 2006-11-28 | 2012-11-06 | Shire Llc | Substituted quinazolines for reducing platelet count |
US7910597B2 (en) * | 2006-11-28 | 2011-03-22 | Shire Llc | Substituted quinazolines |
KR20110017903A (ko) * | 2008-06-04 | 2011-02-22 | 테일크리스 바이오쎄러퓨틱스 아이엔씨. | 플라스민의 제조를 위한 조성물, 방법 및 키트 |
PL2403865T3 (pl) | 2009-03-03 | 2016-01-29 | Grifols Therapeutics Inc | Sposoby wytwarzania plazminogenu |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US2784145A (en) * | 1955-11-22 | 1957-03-05 | American Cyanamid Co | Recovery of streptokinase |
US3226304A (en) * | 1960-10-24 | 1965-12-28 | American Cyanamid Co | High purity streptokinase and process for preparing same |
US3419472A (en) * | 1960-10-24 | 1968-12-31 | American Cyanamid Co | Process for preparing streptokinaserich material |
US3255094A (en) * | 1963-01-10 | 1966-06-07 | Baxter Laboratories Inc | Method for purification of streptokinase |
US3444045A (en) * | 1966-09-20 | 1969-05-13 | American Cyanamid Co | Process for purifying streptokinase |
US4381346A (en) * | 1979-11-13 | 1983-04-26 | Huasin Syed S | Isolation of plasminogen activators useful as therapeutic and diagnostic agents |
-
1988
- 1988-10-19 GB GB888824496A patent/GB8824496D0/en active Pending
-
1989
- 1989-10-17 ZA ZA897849A patent/ZA897849B/xx unknown
- 1989-10-17 US US07/422,430 patent/US5334384A/en not_active Expired - Lifetime
- 1989-10-17 DE DE68914242T patent/DE68914242T2/de not_active Expired - Fee Related
- 1989-10-17 AT AT89310648T patent/ATE103633T1/de not_active IP Right Cessation
- 1989-10-17 EP EP89310648A patent/EP0365278B1/en not_active Expired - Lifetime
- 1989-10-17 NZ NZ231039A patent/NZ231039A/en unknown
- 1989-10-17 IE IE333889A patent/IE63478B1/en not_active IP Right Cessation
- 1989-10-17 AU AU42964/89A patent/AU622492B2/en not_active Ceased
- 1989-10-17 DK DK515889A patent/DK515889A/da not_active Application Discontinuation
- 1989-10-17 CA CA002000850A patent/CA2000850A1/en not_active Abandoned
- 1989-10-17 ES ES89310648T patent/ES2062029T3/es not_active Expired - Lifetime
- 1989-10-18 KR KR1019890015025A patent/KR0136613B1/ko not_active IP Right Cessation
- 1989-10-18 PT PT92024A patent/PT92024B/pt not_active IP Right Cessation
- 1989-10-18 JP JP1269272A patent/JP2837467B2/ja not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
GB8824496D0 (en) | 1988-11-23 |
EP0365278B1 (en) | 1994-03-30 |
AU622492B2 (en) | 1992-04-09 |
DK515889A (da) | 1990-04-20 |
JPH02128688A (ja) | 1990-05-17 |
PT92024A (pt) | 1990-04-30 |
KR0136613B1 (ko) | 1998-04-25 |
DE68914242D1 (de) | 1994-05-05 |
ATE103633T1 (de) | 1994-04-15 |
IE63478B1 (en) | 1995-04-19 |
NZ231039A (en) | 1991-07-26 |
ZA897849B (en) | 1990-10-31 |
JP2837467B2 (ja) | 1998-12-16 |
EP0365278A2 (en) | 1990-04-25 |
US5334384A (en) | 1994-08-02 |
DE68914242T2 (de) | 1994-07-07 |
PT92024B (pt) | 1995-08-09 |
CA2000850A1 (en) | 1990-04-19 |
DK515889D0 (da) | 1989-10-17 |
EP0365278A3 (en) | 1991-08-28 |
AU4296489A (en) | 1990-04-26 |
ES2062029T3 (es) | 1994-12-16 |
IE893338L (en) | 1990-04-19 |
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A201 | Request for examination | ||
E902 | Notification of reason for refusal | ||
N231 | Notification of change of applicant | ||
E701 | Decision to grant or registration of patent right | ||
GRNT | Written decision to grant | ||
LAPS | Lapse due to unpaid annual fee |