KR900006512A - Way - Google Patents

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Publication number
KR900006512A
KR900006512A KR1019890015025A KR890015025A KR900006512A KR 900006512 A KR900006512 A KR 900006512A KR 1019890015025 A KR1019890015025 A KR 1019890015025A KR 890015025 A KR890015025 A KR 890015025A KR 900006512 A KR900006512 A KR 900006512A
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KR
South Korea
Prior art keywords
mixture
reagent
group
contaminating protein
pyridylthio
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Application number
KR1019890015025A
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Korean (ko)
Other versions
KR0136613B1 (en
Inventor
조오지이프 매닉스 크리스토퍼
앤소니 고드윈 스미스 리차아드
존 루이스 세리
스탠리 하아버 줄리안
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비이참 그루우프 피이엘시이
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Publication of KR900006512A publication Critical patent/KR900006512A/en
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Publication of KR0136613B1 publication Critical patent/KR0136613B1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/315—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Streptococcus (G), e.g. Enterococci
    • C07K14/3153—Streptokinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/814—Enzyme separation or purification
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/814—Enzyme separation or purification
    • Y10S435/815—Enzyme separation or purification by sorption
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/885—Streptococcus

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Glass Compositions (AREA)
  • Iron Core Of Rotating Electric Machines (AREA)
  • Steroid Compounds (AREA)
  • Removal Of Specific Substances (AREA)

Abstract

A process for the separation of streptokinase from contaminating proteins in a streptokinase-containing mixture, which comprises treating the mixture with a reducing agent to reduce disulphide bridges in the contaminating proteins to free thiol groups, contacting the mixture with a reagent R-X wherein R is a group capable of reacting with a free thiol group and X is a group R<1> capable of reacting with a free thiol group or is a thiol-containing matrix, and thereafter separating the resulting chemically modified contaminating proteins from the mixture to provide streptokinase in a form substantially free of contaminating proteins.

Description

방 법Way

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

Claims (10)

환원제로 혼합물을 처리하여 오염 단백질내 디설파이드 브릿지를 유리 티올기로 환원시키고, 시약 R-X(여기서, R은 유리 티올기와 반응시킬 수 있는 기이고, X는 유리 티올기와 반응시킬 수 있는 기 R'이거나 티올-함유 메트릭스이다)와 혼합물을 접촉시키고 난후, 결과 형성된 화학적으로 개질시킨 오염 단백질을 혼합물로 부터 분리시켜 오염 단백질이 실질적으로 없는 형태로 스트랩토키나아제를 분리시키는 방법.The mixture is treated with a reducing agent to reduce the disulfide bridge in the contaminating protein to a free thiol group, with reagent RX (where R is a group capable of reacting with a free thiol group, and X is a group R ′ or thiol- capable of reacting with a free thiol group). Contacting the mixture with a matrix, and then separating the resulting chemically modified contaminating protein from the mixture to separate the straptokinase in a form substantially free of contaminating protein. 제1항에 있어서, 환원제가 디티오트레이톨인 방법.The method of claim 1 wherein the reducing agent is dithiothreitol. 제1항 또는 제2항에 있어서, R 및 R'기가 5-니트로-2-피리딜티오 5-카르복시 -2-피리딜티오 2-피리딜티오 4-피리딜티오 2-벤조티아졸릴티오 4-니트로-3-카르복시페닐티오 및 상기 피리딜기의 N-산화물로 부터 선택되는 방법.The R and R 'groups according to claim 1 or 2, wherein 5-nitro-2-pyridylthio 5-carboxy-2-pyridylthio 2-pyridylthio 4-pyridylthio 2-benzothiazolylthio 4 -Nitro-3-carboxyphenylthio and N-oxides of the pyridyl groups. 제1항 내지 제3항중 어느 한 항에 있어서, 시약 R-X가 R-R'형태인 방법.The method of claim 1, wherein the reagent R-X is in the form R-R ′. 제4항에 있어서, 시약 R-R'이 2-2'-디피리틸디설파이드인 방법.The method of claim 4, wherein the reagent R-R 'is 2-2'-dipyridyldisulfide. 제4항 또는 제5항에 있어서, 시약 R-R'의 농도가 10-200mM인 방법.The method of claim 4 or 5, wherein the concentration of reagent R-R 'is 10-200 mM. 제2항 내지 제6항중 어느 한 항에 있어서, 시약 R-R'과 환원시킨 혼합물과의 반응이 pH 6.0-8.5에서 수행되는 방법.The process of claim 2, wherein the reaction of reagent R-R ′ with the reduced mixture is carried out at pH 6.0-8.5. 제4항 내지 제7항중 어느 한 항에 있어서, 시약 R-R'과 환원시킨 혼합물과의 반응이 5-35℃에서 수행되는 방법.The process according to claim 4, wherein the reaction of the reagent R-R ′ with the reduced mixture is carried out at 5-35 ° C. 9. 제4항 내지 제8항중 어느 한 항에 있어서, 결과형성된 화학적으로 개질시킨 오염 단백질이 여과, 침강, 원심분리법에 의해 또는 크로마토그래피 컬럼 내체류에 의해 분리시킨 침전물을 형성시키는 방법.The method of claim 4, wherein the resultant chemically modified contaminating protein forms a precipitate separated by filtration, sedimentation, centrifugation, or by chromatography column residence. 제9항에 있어서, 화학적으로 개질시킨 잔류 오염 단백질이 티올 교환 크로마토그래피에 의해 제거되는 방법.The method of claim 9, wherein the chemically modified residual contaminating protein is removed by thiol exchange chromatography. ※ 참고사항 최초출원 내용에 의하여 공개하는 것임.※ Note The disclosure is based on the initial application.
KR1019890015025A 1988-10-19 1989-10-18 Process for purification of streptokinase KR0136613B1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB888824496A GB8824496D0 (en) 1988-10-19 1988-10-19 Process
GB8824496.7 1988-10-19

Publications (2)

Publication Number Publication Date
KR900006512A true KR900006512A (en) 1990-05-08
KR0136613B1 KR0136613B1 (en) 1998-04-25

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KR1019890015025A KR0136613B1 (en) 1988-10-19 1989-10-18 Process for purification of streptokinase

Country Status (15)

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US (1) US5334384A (en)
EP (1) EP0365278B1 (en)
JP (1) JP2837467B2 (en)
KR (1) KR0136613B1 (en)
AT (1) ATE103633T1 (en)
AU (1) AU622492B2 (en)
CA (1) CA2000850A1 (en)
DE (1) DE68914242T2 (en)
DK (1) DK515889A (en)
ES (1) ES2062029T3 (en)
GB (1) GB8824496D0 (en)
IE (1) IE63478B1 (en)
NZ (1) NZ231039A (en)
PT (1) PT92024B (en)
ZA (1) ZA897849B (en)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE69929310T2 (en) 1998-02-05 2006-08-03 Glaxosmithkline Biologicals S.A. TUMOR ASSOCIATED ANTIGEN DERIVATIVES OF THE MAGE FAMILY, NUCLEIC ACID SEQUENCES THAT CODE FOR THE PREPARATION OF FUSION PROTEINS AND COMPOSITIONS FOR VACCINATION
US6388073B1 (en) 2000-07-26 2002-05-14 Shire Us Inc. Method for the manufacture of anagrelide
AU2002211669A1 (en) 2000-10-12 2002-04-22 Robert Corcoran Purification of substance by reaction affinity chromatography
KR20020007231A (en) * 2001-08-01 2002-01-26 이병철 The purification of streptokinase
US20060030574A1 (en) * 2004-08-04 2006-02-09 Shire Holdings Ag Quinazoline derivatives useful for the treatment of peripheral arterial disease and as phosphodiesterase inhibitors
US7700608B2 (en) 2004-08-04 2010-04-20 Shire Holdings Ag Quinazoline derivatives and their use in the treatment of thrombocythemia
US8304420B2 (en) 2006-11-28 2012-11-06 Shire Llc Substituted quinazolines for reducing platelet count
US7910597B2 (en) * 2006-11-28 2011-03-22 Shire Llc Substituted quinazolines
KR20160110544A (en) * 2008-06-04 2016-09-21 그리폴스 테라퓨틱스 인코포레이티드 Composition, method and kit for preparing plasmin
PT2403865E (en) 2009-03-03 2015-11-18 Grifols Therapeutics Inc Methods for preparing plasminogen

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2784145A (en) * 1955-11-22 1957-03-05 American Cyanamid Co Recovery of streptokinase
US3226304A (en) * 1960-10-24 1965-12-28 American Cyanamid Co High purity streptokinase and process for preparing same
US3419472A (en) * 1960-10-24 1968-12-31 American Cyanamid Co Process for preparing streptokinaserich material
US3255094A (en) * 1963-01-10 1966-06-07 Baxter Laboratories Inc Method for purification of streptokinase
US3444045A (en) * 1966-09-20 1969-05-13 American Cyanamid Co Process for purifying streptokinase
US4381346A (en) * 1979-11-13 1983-04-26 Huasin Syed S Isolation of plasminogen activators useful as therapeutic and diagnostic agents

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DK515889A (en) 1990-04-20
CA2000850A1 (en) 1990-04-19
AU4296489A (en) 1990-04-26
ZA897849B (en) 1990-10-31
EP0365278B1 (en) 1994-03-30
DK515889D0 (en) 1989-10-17
KR0136613B1 (en) 1998-04-25
IE893338L (en) 1990-04-19
EP0365278A3 (en) 1991-08-28
AU622492B2 (en) 1992-04-09
DE68914242T2 (en) 1994-07-07
GB8824496D0 (en) 1988-11-23
DE68914242D1 (en) 1994-05-05
ATE103633T1 (en) 1994-04-15
NZ231039A (en) 1991-07-26
ES2062029T3 (en) 1994-12-16
JPH02128688A (en) 1990-05-17
PT92024B (en) 1995-08-09
JP2837467B2 (en) 1998-12-16
IE63478B1 (en) 1995-04-19
US5334384A (en) 1994-08-02
PT92024A (en) 1990-04-30
EP0365278A2 (en) 1990-04-25

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