KR900003372A - 이. 콜라이 및 그람-음성 세균의 외막 단백질 a를 함유한 융합단백질 및 이의 발현방법 - Google Patents
이. 콜라이 및 그람-음성 세균의 외막 단백질 a를 함유한 융합단백질 및 이의 발현방법 Download PDFInfo
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- KR900003372A KR900003372A KR1019890012043A KR890012043A KR900003372A KR 900003372 A KR900003372 A KR 900003372A KR 1019890012043 A KR1019890012043 A KR 1019890012043A KR 890012043 A KR890012043 A KR 890012043A KR 900003372 A KR900003372 A KR 900003372A
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- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
- C07K14/245—Escherichia (G)
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- C—CHEMISTRY; METALLURGY
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- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
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- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/305—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Micrococcaceae (F)
- C07K14/31—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Micrococcaceae (F) from Staphylococcus (G)
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
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- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/035—Fusion polypeptide containing a localisation/targetting motif containing a signal for targeting to the external surface of a cell, e.g. to the outer membrane of Gram negative bacteria, GPI- anchored eukaryote proteins
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
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- C07K2319/50—Fusion polypeptide containing protease site
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- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/55—Fusion polypeptide containing a fusion with a toxin, e.g. diphteria toxin
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- C12N2760/00011—Details
- C12N2760/16011—Orthomyxoviridae
- C12N2760/16111—Influenzavirus A, i.e. influenza A virus
- C12N2760/16122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2770/00011—Details
- C12N2770/32011—Picornaviridae
- C12N2770/32111—Aphthovirus, e.g. footandmouth disease virus
- C12N2770/32122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
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- Proteomics, Peptides & Aminoacids (AREA)
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Abstract
내용 없음
Description
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 플라스미드 pHK 236의 작제도이다.
제2a도는 플라스미드 pHS 51의 합성단계 개략도이다.
제2b도는 플라스미드 pHS 53 의 합성단계 개략도이다.
제2c도는 플라스미드 pHS 56 의 합성단계 개략도이다.
제2b도는 플라스미드 pHS64의 합성단계 개략도이다.
제3도는 플라스미드 pinf 4-6의 작제도이다.
Claims (12)
- 하나의 파트너는 그림 - 음성 세균의 외막 단백질 A(OmpA)를 함유하고 다른 하나는 20개 이상의 아미노산으로 이루어진 단백질을 함유하는 융합 단백질
- 제1항에 있어서,OmpA가 이 콜라이 또는 쉬겔라 다이센테리애(S.dyenteriae)로부터 유도되거나 이들 OmpA의 하이 브리드로 이루어진 융합 단백질.
- 20개 이상의 아미노산으로 이루어진 단백질이 그림 - 음성 세균의 OmpA 의 회측첨(apical)영역 4개중 하나에 삽입된 융밥 단백질.
- 제3항에 있어서, OmpA가 이. 콜라이 또는 쉬겔라다이센테이애로부터 유도되거나 이들 OmpA의 하이 브리드로 이루어진 융합 단백질.
- 제3항에 있어서, 인플루엔자 혈구응집소의 아미노 - 또는 카복시 - 말단 영역(약 아미노산 1내지 50 및 아미노산 280 내지 510)으로부터의 3차원성 접힘 영역에 상응하는 스톡(stalk)이 삽입된 단백질의 아미노 - 말단앞에 또는 카복시 - 말단 뒤에 추가로 삽입된 융합 단백질.
- 제3항에 있어서, 추가로 프로테아제에 의해 절단될 수 있는 서열이 삽입 단백질의 아미노 - 말단 앞에 및 카복시 말단 뒤에 위치하는 융합 단백질.
- 제6항에 있어서. 콜라게나제에 의해 절단될 수 있는 서열이 삽입된 융합 단백질.
- 제1항 내지 7항중 어느 한 항에 따른 융합 단백질을 암호화하는 벡터로 형질 전환된 그람 - 음성 세균.
- 관련된 펩티이드 또는 단백질 암호화 DNA 서열을 정확한 판독 프레임중에 함께 결합시키고, 발현 벡터에 연결시킨후, 이 발현 벡터로 그림 - 음성 세균을 형질전환시켜 이 성질 전환체 중에서 발현시킴을 특징으로 하여, 제 1항 내지 7항중 어느 한 항에 따른 융합 단백질을 제조하는 방법.
- 제9항에 있어서, 이, 콜라이, 사로낼라 티파이(S.typhi)또는 살모넬라 티피무리움(S.typhimurium)세포를 제1항 내지 7항중 어느 한 항에 따른 융합 단백질을 발현시키기 위한 하이브리드 플라스미드로 형질 전환시키는 방법.
- 제1항 내지 7항중 어느 한 항에 따른 융합 단백질의 백신으로서의 용도.
- 유도물질로 유도시킨 제 8항에 따른 형질전환된 그림 - 음성 세균의 경구용 생백신으로서의 용도.※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE3828666A DE3828666A1 (de) | 1988-08-24 | 1988-08-24 | Expression funktionsfaehiger homologer und heterologer proteine auf der aeusseren membran von e.coli und anderen gramnegativen bakterien |
DEP3828666.1 | 1988-08-24 |
Publications (1)
Publication Number | Publication Date |
---|---|
KR900003372A true KR900003372A (ko) | 1990-03-26 |
Family
ID=6361472
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
KR1019890012043A KR900003372A (ko) | 1988-08-24 | 1989-08-24 | 이. 콜라이 및 그람-음성 세균의 외막 단백질 a를 함유한 융합단백질 및 이의 발현방법 |
Country Status (8)
Country | Link |
---|---|
EP (1) | EP0355737A3 (ko) |
JP (1) | JPH02135095A (ko) |
KR (1) | KR900003372A (ko) |
AU (1) | AU614174B2 (ko) |
DE (1) | DE3828666A1 (ko) |
DK (1) | DK416489A (ko) |
FI (1) | FI893932A (ko) |
PT (1) | PT91520A (ko) |
Families Citing this family (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5821088A (en) * | 1990-05-11 | 1998-10-13 | Siga Pharmaceuticals, Inc. | Use of gram-positive bacteria to express recombinant proteins |
EP0474891A1 (en) * | 1990-09-08 | 1992-03-18 | BEHRINGWERKE Aktiengesellschaft | Vectors for expression of malarial antigens on the surface of Salmonella vaccine strains |
WO1993010246A1 (en) * | 1991-11-15 | 1993-05-27 | Board Of Regents, The University Of Texas System | Membrane expression of heterologous genes |
US5356797A (en) * | 1991-11-15 | 1994-10-18 | Board Of Regents, The University Of Texas | Membrane expression of heterologous genes |
WO1993024636A1 (en) * | 1992-05-29 | 1993-12-09 | The University Of British Columbia | Use of protein oprf for bacterial cell surface expression of oligopeptides |
FR2718452B1 (fr) | 1994-04-06 | 1996-06-28 | Pf Medicament | Elément d'immunogène, agent immunogène, composition pharmaceutique et procédé de préparation. |
JP4108122B2 (ja) * | 1995-05-15 | 2008-06-25 | アボット・ラボラトリーズ | 異種蛋白質の埋込発現 |
GB9718591D0 (en) * | 1997-09-03 | 1997-11-05 | Zeneca Ltd | Methods |
NO20010238D0 (no) * | 2001-01-12 | 2001-01-12 | Unifob Stiftelsen Universitets | Systemer for presentasjon på overflaten til metanotrofe bakterier |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
IL60184A (en) * | 1979-05-31 | 1984-05-31 | Schering Ag | Process for the specific cleavage of protein sequences from proteins |
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1988
- 1988-08-24 DE DE3828666A patent/DE3828666A1/de not_active Withdrawn
-
1989
- 1989-08-18 EP EP19890115263 patent/EP0355737A3/de not_active Withdrawn
- 1989-08-22 FI FI893932A patent/FI893932A/fi not_active Application Discontinuation
- 1989-08-23 AU AU40160/89A patent/AU614174B2/en not_active Ceased
- 1989-08-23 PT PT91520A patent/PT91520A/pt unknown
- 1989-08-23 DK DK416489A patent/DK416489A/da not_active Application Discontinuation
- 1989-08-24 KR KR1019890012043A patent/KR900003372A/ko not_active Application Discontinuation
- 1989-08-24 JP JP1218439A patent/JPH02135095A/ja active Pending
Also Published As
Publication number | Publication date |
---|---|
EP0355737A2 (de) | 1990-02-28 |
PT91520A (pt) | 1990-03-08 |
FI893932A (fi) | 1990-02-25 |
EP0355737A3 (de) | 1991-10-30 |
DK416489D0 (da) | 1989-08-23 |
AU614174B2 (en) | 1991-08-22 |
JPH02135095A (ja) | 1990-05-23 |
DE3828666A1 (de) | 1990-03-01 |
DK416489A (da) | 1990-02-25 |
AU4016089A (en) | 1990-03-01 |
FI893932A0 (fi) | 1989-08-22 |
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