KR900003372A - 이. 콜라이 및 그람-음성 세균의 외막 단백질 a를 함유한 융합단백질 및 이의 발현방법 - Google Patents

이. 콜라이 및 그람-음성 세균의 외막 단백질 a를 함유한 융합단백질 및 이의 발현방법 Download PDF

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KR900003372A
KR900003372A KR1019890012043A KR890012043A KR900003372A KR 900003372 A KR900003372 A KR 900003372A KR 1019890012043 A KR1019890012043 A KR 1019890012043A KR 890012043 A KR890012043 A KR 890012043A KR 900003372 A KR900003372 A KR 900003372A
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fusion protein
protein
ompa
coli
amino acids
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KR1019890012043A
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호봄 게르트
피스토르 주잔
아놀드 노베르트
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필립 스타인, 헤리베르트 북
베링베르케 아크티엔게젤샤프트
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Publication of KR900003372A publication Critical patent/KR900003372A/ko

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Abstract

내용 없음

Description

이.콜라이 및 그람- 음성 세균의 외막 단백질 A를 함유한 융합단백질 및 이의 발현방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 플라스미드 pHK 236의 작제도이다.
제2a도는 플라스미드 pHS 51의 합성단계 개략도이다.
제2b도는 플라스미드 pHS 53 의 합성단계 개략도이다.
제2c도는 플라스미드 pHS 56 의 합성단계 개략도이다.
제2b도는 플라스미드 pHS64의 합성단계 개략도이다.
제3도는 플라스미드 pinf 4-6의 작제도이다.

Claims (12)

  1. 하나의 파트너는 그림 - 음성 세균의 외막 단백질 A(OmpA)를 함유하고 다른 하나는 20개 이상의 아미노산으로 이루어진 단백질을 함유하는 융합 단백질
  2. 제1항에 있어서,OmpA가 이 콜라이 또는 쉬겔라 다이센테리애(S.dyenteriae)로부터 유도되거나 이들 OmpA의 하이 브리드로 이루어진 융합 단백질.
  3. 20개 이상의 아미노산으로 이루어진 단백질이 그림 - 음성 세균의 OmpA 의 회측첨(apical)영역 4개중 하나에 삽입된 융밥 단백질.
  4. 제3항에 있어서, OmpA가 이. 콜라이 또는 쉬겔라다이센테이애로부터 유도되거나 이들 OmpA의 하이 브리드로 이루어진 융합 단백질.
  5. 제3항에 있어서, 인플루엔자 혈구응집소의 아미노 - 또는 카복시 - 말단 영역(약 아미노산 1내지 50 및 아미노산 280 내지 510)으로부터의 3차원성 접힘 영역에 상응하는 스톡(stalk)이 삽입된 단백질의 아미노 - 말단앞에 또는 카복시 - 말단 뒤에 추가로 삽입된 융합 단백질.
  6. 제3항에 있어서, 추가로 프로테아제에 의해 절단될 수 있는 서열이 삽입 단백질의 아미노 - 말단 앞에 및 카복시 말단 뒤에 위치하는 융합 단백질.
  7. 제6항에 있어서. 콜라게나제에 의해 절단될 수 있는 서열이 삽입된 융합 단백질.
  8. 제1항 내지 7항중 어느 한 항에 따른 융합 단백질을 암호화하는 벡터로 형질 전환된 그람 - 음성 세균.
  9. 관련된 펩티이드 또는 단백질 암호화 DNA 서열을 정확한 판독 프레임중에 함께 결합시키고, 발현 벡터에 연결시킨후, 이 발현 벡터로 그림 - 음성 세균을 형질전환시켜 이 성질 전환체 중에서 발현시킴을 특징으로 하여, 제 1항 내지 7항중 어느 한 항에 따른 융합 단백질을 제조하는 방법.
  10. 제9항에 있어서, 이, 콜라이, 사로낼라 티파이(S.typhi)또는 살모넬라 티피무리움(S.typhimurium)세포를 제1항 내지 7항중 어느 한 항에 따른 융합 단백질을 발현시키기 위한 하이브리드 플라스미드로 형질 전환시키는 방법.
  11. 제1항 내지 7항중 어느 한 항에 따른 융합 단백질의 백신으로서의 용도.
  12. 유도물질로 유도시킨 제 8항에 따른 형질전환된 그림 - 음성 세균의 경구용 생백신으로서의 용도.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019890012043A 1988-08-24 1989-08-24 이. 콜라이 및 그람-음성 세균의 외막 단백질 a를 함유한 융합단백질 및 이의 발현방법 KR900003372A (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE3828666A DE3828666A1 (de) 1988-08-24 1988-08-24 Expression funktionsfaehiger homologer und heterologer proteine auf der aeusseren membran von e.coli und anderen gramnegativen bakterien
DEP3828666.1 1988-08-24

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EP (1) EP0355737A3 (ko)
JP (1) JPH02135095A (ko)
KR (1) KR900003372A (ko)
AU (1) AU614174B2 (ko)
DE (1) DE3828666A1 (ko)
DK (1) DK416489A (ko)
FI (1) FI893932A (ko)
PT (1) PT91520A (ko)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5821088A (en) * 1990-05-11 1998-10-13 Siga Pharmaceuticals, Inc. Use of gram-positive bacteria to express recombinant proteins
EP0474891A1 (en) * 1990-09-08 1992-03-18 BEHRINGWERKE Aktiengesellschaft Vectors for expression of malarial antigens on the surface of Salmonella vaccine strains
AU3130893A (en) * 1991-11-15 1993-06-15 Board Of Regents, The University Of Texas System Membrane expression of heterologous genes
US5356797A (en) * 1991-11-15 1994-10-18 Board Of Regents, The University Of Texas Membrane expression of heterologous genes
WO1993024636A1 (en) * 1992-05-29 1993-12-09 The University Of British Columbia Use of protein oprf for bacterial cell surface expression of oligopeptides
FR2718452B1 (fr) 1994-04-06 1996-06-28 Pf Medicament Elément d'immunogène, agent immunogène, composition pharmaceutique et procédé de préparation.
ATE406451T1 (de) * 1995-05-15 2008-09-15 Abbott Lab Verankerte expression heterologer proteine
GB9718591D0 (en) * 1997-09-03 1997-11-05 Zeneca Ltd Methods
NO20010238D0 (no) * 2001-01-12 2001-01-12 Unifob Stiftelsen Universitets Systemer for presentasjon på overflaten til metanotrofe bakterier

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL60184A (en) * 1979-05-31 1984-05-31 Schering Ag Process for the specific cleavage of protein sequences from proteins

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DE3828666A1 (de) 1990-03-01
AU4016089A (en) 1990-03-01
FI893932A0 (fi) 1989-08-22
JPH02135095A (ja) 1990-05-23
DK416489D0 (da) 1989-08-23
FI893932A (fi) 1990-02-25
EP0355737A3 (de) 1991-10-30
PT91520A (pt) 1990-03-08
EP0355737A2 (de) 1990-02-28
DK416489A (da) 1990-02-25
AU614174B2 (en) 1991-08-22

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