KR900001424B1 - Dipping method of indian corn by fermentation lactobacillus - Google Patents

Dipping method of indian corn by fermentation lactobacillus Download PDF

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KR900001424B1
KR900001424B1 KR1019870012489A KR870012489A KR900001424B1 KR 900001424 B1 KR900001424 B1 KR 900001424B1 KR 1019870012489 A KR1019870012489 A KR 1019870012489A KR 870012489 A KR870012489 A KR 870012489A KR 900001424 B1 KR900001424 B1 KR 900001424B1
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corn
lactic acid
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immersion
fermentation
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모도이찌 시게히로
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모도이찌 시게히로
배윤숙
진정권
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Abstract

A by-product from corn is prepared using lactic ferments. Thus, 2kg of corn is treated with 1 litre of lactic ferments cultured in wheat flur at 47oC for 35-45 hrs. In this process, the water content of the corn is changed from 13.5% into 42%. The first lactic ferments solution is blended with a mixture containing corn starch and wheat flour and formented for 48 hrs to give the second lactic ferments solution. Corn is soaked into the second lactic ferments solution to give a by-product from the corn.

Description

발효유산 활성균액에 의한 옥수수의 침지법Soaking of Corn by Fermented Lactic Acid Bacteria

제1도는 본 발명의 공정도이다.1 is a process diagram of the present invention.

* 도면의 주요부분에 대한 부호의 설명* Explanation of symbols for main parts of the drawings

1 : 원료 침지조 2 : 원료 침지조1: raw material immersion tank 2: raw material immersion tank

3 : 침지액 펌프 4-1~4-3 : 침지액 발효탱크3: immersion liquid pump 4-1 ~ 4-3: immersion liquid fermentation tank

5-1~5-4 : 폐액 발효탱크 6 : 원액탱크5-1 ~ 5-4: Waste solution fermentation tank 6: Stock solution tank

7 : 소맥분탱크 8 : 용해기7: wheat flour tank 8: melter

9 : 연속가열기 10 : 체류탱크9: continuous heater 10: retention tank

11 : 연속 냉각기 12 : 침지액탱크11: continuous cooler 12: immersion liquid tank

13 : 공정수액탱크13: process sap tank

본 발명은 옥수수 부산물을 제조함에 있어서 종래의 아황산 0.3%수에 의한 침지법과는 달리 아황산을 사용치 않고 옥수수알을 유산발효액(pH4.0~4.5정도의 액)에 침지하여서 부산물을 제조하는 방법에 관한 것으로, 3%소맥분(3등 소맥분) 용액을 100℃에서 3분 정도로 가열하여 호화한 다음 47℃로 냉각하여 주박(酒粕) 및 소맥분중의 유산균을 종균으로 하여서된 유산발효액으로 유산발효시키되 옥수수 원료의 1/2량 정도로 1차The present invention is to prepare a by-product by immersing corn grains in lactic fermentation solution (pH 4.0 ~ 4.5 solution) without the use of sulfurous acid, unlike the conventional immersion method with 0.3% sulfurous acid in the production of corn by-products The 3% wheat flour (third class wheat flour) solution was heated at 100 ° C. for about 3 minutes, then cooled to 47 ° C., and then fermented with lactic acid fermentation broth using lactic acid bacteria in sake lees and wheat flour. Primary about 1/2 of raw material

옥수수 침지는 미국에서 60~70년전에 시작하여 적어도 1940년까지는 52℃~53℃아황산 0.3%액을 사용하였다. 이는 아황산의 pH와 고온때문에 48시간 경과하면 아황산에 의한 입(粒) 내부의 용출성 물질이 용출하고 용출된 물질인 옥수수 침지액을 52℃~53℃로서 배낸것을 후발효에 의해 유산발효시켜 사용하였다.Corn soaking started in the United States 60-70 years ago and used at least 52% -53 ° C of sulfurous acid 0.3% until at least 1940. After 48 hours due to the pH and high temperature of sulfurous acid, the eluted substance inside the granules by sulfurous acid elutes, and the fermented corn soaked liquid as 52 ℃ ~ 53 ℃ is fermented by post-fermentation. Used.

1948년경 일본식품화공(주)의 가와베씨에 의한 46℃~47℃의 저온장시간 침지방법에 특허가 되기에 이르러 세계적으로 유산발효를 병용한 침지방법으로 개량 발전되었다.In 1948, it was patented by Kawabe of Nippon Food Chemical Co., Ltd. for a long time dipping method of 46 ° C to 47 ° C.

그러나 이 시점부터 현재에 이르기까지 옥수수 침지법으로서의 0.3%아황산 사용은 변하지 않고 지금까지 사용되고 있다.However, from this point to the present, the use of 0.3% sulfurous acid as a corn dipping method has not changed and has been used until now.

그러나 유산균은 pH3~3.5이하로는 가사(假死)상태로 되어 증식은 불가능하다. 아황산은 유산발효가 완성하게 되는 그 원료의 변질을 방지하기 위해서이고 52℃~53℃의 경우 지금도 모든 당사자는 그것을 믿고 있는 것이다.However, the lactic acid bacteria are in the domestic state (pH) below pH 3 ~ 3.5, the proliferation is impossible. Sulfurous acid is to prevent the deterioration of the raw material that the lactic fermentation is completed, and in the case of 52 ℃ ~ 53 ℃ all parties still believe it.

현재 아황산의 방법에도 침지액을 47℃ 정도로 증기 가열하면서 순환을 계속하면 pH는 점점 상승하여 pH3.5이상이 되면 유산이 증식하기 시작하여 옥수수 내부의 영양분의 용출이 시작되어 아황산이 감소하고 발효가 왕성하게 되며, 옥수수 수분이 42%정도가 되면 침지완료한다.In the current method of sulfurous acid, if the circulation is continued while steaming the immersion liquid at about 47 ° C, the pH gradually increases, and when the pH is above 3.5, lactic acid begins to increase, and nutrients in the corn begin to evaporate. When the corn is about 42%, the immersion is completed.

이때 유산침지액의 pH가 4~4.5(47℃)이면 다른 잡균은 번식하지 않고 부패등의At this time, if pH of lactic acid immersion liquid is 4 ~ 4.5 (47 ℃), other various germs do not breed and

아황산을 침지에 사용하는 경우의 중요한 결점을 들면 다음과 같다.Important drawbacks when using sulfurous acid for dipping include:

(1) 아황산수를 제조하는데에 아황산 제조장치가 필요하고 흡수율 80~90%와 손실이 있으며, 배기가스중에 아황산 가스로서 유출된다.(1) A sulfurous acid production apparatus is necessary for producing sulfurous acid water, has an absorption rate of 80 to 90%, and loss, and is discharged as sulfurous acid gas in the exhaust gas.

(2) 아황산수는 저온에서도 아황산 가스로서 방출된다. 침지공장은 물론 마쇄공장, 공정수와 모든 장소에서 아황산의 악취가 있고 건강상 좋지 않다.(2) Sulfurous water is released as sulfurous acid gas even at low temperatures. There are odors of sulfurous acid in the dipping plant as well as in the grinding mill, in the process water and in all places.

(3) 장치에는 모두 스테인레스 강을 사용해야만 한다.(3) All devices must be made of stainless steel.

(4) 모든 제품의 다소를 불문하고 분석치가 삼산화황이 검출되는 식품의 경우는 표시하지 않으면 안된다.(4) In the case of foods where sulfur trioxide is detected, analysis value must be displayed regardless of some products.

(5) 모든 부산물에는 매운맛, 쓴맛등을 느낄 수 있다. 특히 옥수수 침지액을 사료화, 식료화한 경우 대단히 부적당하다.(5) All the by-products can be spicy, bitter. In particular, corn steep liquor is very inadequate when fed and foodized.

본 발명은 이와 같은 결정을 보완하고 유산액만을 사용하여 옥수수 부산물을 제조할 수 있게 한 것이다.The present invention complements this crystal and enables the production of corn by-products using only lactic acid.

본 발명을 공정별로 설명하면 다음과 같다.Referring to the present invention by the process as follows.

1) 1차 발효유산 활성균액 제조공정.1) Production process of primary fermented lactic acid active bacteria solution.

3중량%의 소맥분 수용액을 100℃, 30분 가열하여 페이스트로 만들고 이를 47℃로 냉각하여 페이스트 100중량%를 기준으로 0.05중량%의 주박과 0.03중량%의 소맥분을 24시간 교반하여 발효유산 활성액을 제조한다.The solution of 3% by weight of wheat flour was heated to 100 ° C. for 30 minutes to form a paste, and then cooled to 47 ° C., whereby fermented lactic acid active liquid was stirred for 24 hours by stirring 0.05% by weight of liquor and 0.03% by weight of wheat flour based on 100% by weight of paste. Manufacture.

2) 발효유산 활성균액 침지공정.2) Fermented lactic acid active bacterial solution dipping process.

옥수수와 상기 발효유산 활성균액을 (2㎏ : 1l)비율로 섞어 47℃에서 35~45시간 지나면 옥수수의 수분함량이 13.5%에서 42%로 되어 발효유산 활성균액 침지공정을 완After mixing corn and the fermented lactic acid active bacteria solution at a ratio of 2 kg: 1 l, the water content of corn is increased from 13.5% to 42% after 47 to 45 hours at 47 ° C.

3) 2차 발효유산 활성균액 제조공정.3) Second fermentation lactic acid active bacterial liquid production process.

공지의 밀링공정시 부산되는 전분액인 콘스타치를 고형분 2%로 희석한액 500l에 별도의 소맥분 2.5~3kg을 첨가하여 pH가 4~4.5로 되도록 원액을 800l로 만든다음 100℃로 6초간 연속가열시키고, 이를 130℃로 1시간 가열한 다음 47℃로 냉각하여 제조된 액 500l에, 1차 발효유산 활성균액 75l을 혼합하면 pH가 5~6정도되며, 이를 48시간 교반하면 발효하여 pH가 3~3.5로 된다.In a known milling process, add 500 ml of cornstarch, a by-product of starch, diluted with 2% solids, and add 2.5 to 3 kg of additional wheat flour to make 800l so that the pH becomes 4 to 4.5. The mixture was heated to 130 ° C. for 1 hour and then cooled to 47 ° C. to 500 l of the liquid prepared by mixing 75 l of the primary fermentation lactic acid active bacteria solution. The pH was about 5-6. After stirring for 48 hours, the pH was 3 It becomes -3.5.

따라서 발효 효율을 상승시키기 위하여 밀스타치 제조시 부산되는 pH5~6의 마쇄폐액과 pH3~3.5의 교반발효액을 혼합하여 pH를 5로 조정하여서 2차 발효유산 활성균액을 제조한다.Therefore, in order to increase the fermentation efficiency, the secondary fermentation lactic acid active bacteria liquid is prepared by adjusting the pH to 5 by mixing the ground waste solution of pH 5 to 6 and the stirred fermentation solution of pH 3 to 3.5, which are by-products produced during millstarch production.

이에 옥수수를 침지시키며, 이와 같이 반복하여 옥수수를 침지할 수 있게 한 것이다.This is soaked with corn, so that it is possible to soak corn repeatedly.

이상과 같이 본 발명은 첫번째 옥수수를 침지할 때는 별도의 발효유산 활성균액을 제조하여 사용하지만 2번째 부터의 옥수수를 침지할 때는 첫번째 옥수수 침지시 나오는 일차유산균액에 밀링시 부산되는 콘스타치를 희석한 액과 소맥분을 혼합하여 가열시켜 호화시킨 다음 냉각시켜서 된 유산활성균액을 혼합시켜 이차 유산균액을 제조하여서 옥수수를 침지할 수 있으므로 아황산(SO2)에 의한 침지공정을 거치지 않고 바로 발효유산 활성균으로 침지할 수가 있으므로 제조공정이 간단하고 제작비가 절감된다.As described above, the present invention prepares and uses a separate fermentation lactobacillus active bacteria liquid when immersing the first corn, but dilutes cornstarch produced by milling with primary lactobacillus liquid when the first corn is immersed. And wheat flour mixed, heated and gelatinized, and then cooled, mixed with lactic acid activated bacteria to prepare a secondary lactic acid bacteria solution, so that corn can be soaked, soaked with fermented lactic acid active bacteria without going through the dipping process with sulfurous acid (SO 2 ). This makes the manufacturing process simple and reduces manufacturing costs.

또한 일차 발효유산 활성균액에 의한 침지공정 시간이 35~45시간이면 침지가 완료되므로 아황산에 의한 48시간 정도의 침지시간이 단축될 수 있다. 이는 아황산에 의In addition, if the immersion process time by the primary fermentation lactic acid active bacteria liquid is 35 to 45 hours soaking is completed, soaking time of about 48 hours by sulfurous acid can be shortened. This is due to sulfurous acid

유산액은 옥수수 입내에 유산균이 침입하여 당질을 유산으로 분해하며, 유산균이 증식함에 따라 단백질을 아미노산으로 변화시켜 주므로 아미노산의 생산도 많다.Lactic acid solution breaks down sugars into lactic acid by invading corn in the mouth, and as lactic acid bacteria proliferate, the protein is converted into amino acids, thus producing many amino acids.

단, 단백질이 소분자량으로 펩타이드형이 가용성인 경우에 한 한다. 유산균이 가장 양호한 pH는 7정도이다.Provided that the peptide is soluble in small molecular weight protein. The best pH for lactic acid bacteria is about 7.

pH7의 경우는 단백도 용해하는 경우가 있고 유산균의 영양으로서 아미노산화해서 이용된다.In the case of pH7, protein may also be dissolved, and it is amino acidized and used as nutrition of lactic acid bacteria.

본 발명에 의한 경우 제품 부산물임에도 불구하고 대부분의 장치는 철재로 가능하고 초기 원가가 크게 차이난다.In the case of the present invention, even though the product is a by-product, most of the devices are made of steel, and the initial cost varies greatly.

특히, 아미노산은 양질의 것을 얻을 수 있다. 또한 침지시간도 단축시키고 공장환경도 양호하다. 이하 실시예를 첨부도면을 참조하면서 상세히 설명한다.In particular, amino acids can be of good quality. In addition, the immersion time is shortened and the plant environment is good. Hereinafter, the embodiments will be described in detail with reference to the accompanying drawings.

실시예는 소규모 공장에서의 실조업을 예로 해서 설명한다. 원료 옥수수 1000㎏을 승강기에 의해 제진하여 원료침지조(1)(2)에 별도로 발효유산 활성균액 탱크(14)에서 제조한 발효유산 활성균액 500l를 투입 침지하면 발효유산 활성균액 침지공정에 의하여 300l의 옥수수 침지액을 얻고 옥수수 침지액 300l중 1/4인 75l를 침지액 펌프(3)에 의해 침지액 발효탱크(4-1)~(4-3)에 넣고, 나머지 3/4인 225l는 폐액발효탱크(5-1)~(5-4)에 넣는다.The embodiment will be described by taking an actual operation in a small factory as an example. When 1000 kg of raw corn is removed by an elevator and 500 l of fermented lactic acid active bacteria liquid prepared in the fermented lactic acid active bacterial liquid tank 14 is immersed in the raw material immersion tank (1) and (2), 300 l of the fermented lactic acid active bacteria liquid is immersed. Corn steep liquor was obtained and 75 liters (1/4 of 300 liters of corn steep liquor) were added to the immersion liquid fermentation tanks (4-1) to (4-3) by the immersion pump (3). Put it into the waste liquid fermentation tanks (5-1) to (5-4).

원액탱크(6)는 800l의 용량으로 해서 단백을 포함한 고형분 2%의 밀스타치 용액 500l를 넣고, 소맥분탱크(7), 용해기(8)를 통해 소맥분탱크에서 공급받은 3등 소맥분 2.5㎏을 넣어 교반한다. pH는 공정수와 같은 4~4.5이다.The stock tank (6) has a capacity of 800 liters, and puts 500 liters of mill starch solution containing 2% solids, including protein, and adds 2.5 kg of third-class wheat flour supplied from the wheat flour tank through the wheat flour tank (7) and the dissolver (8). Stir. pH is 4 ~ 4.5 same as process water.

연속가열기(9)에 의해 100℃에서 6초동안 가열하고 증기배출에 의해 130℃로 한다(완전 호화한다). 체류탱크(10)은 2㎏/㎠압력에서 1시간 체류하는 탱크이고 600~1000l를 넣는다.It heats at 100 degreeC for 6 second with the continuous heater 9, and it is made 130 degreeC by vapor discharge (completely gelatinized). The retention tank 10 is a tank for 1 hour at 2 kg / cm 2 pressure and puts 600 ~ 1000l.

이와 같은 이유는 1시간 체류함으로서 호화된 전분액을 완전 액화시켜 유산발효를 용이하게 하기 위함이다.The reason for this is to facilitate lactic acid fermentation by completely liquefying the gelatinized starch liquid by staying for 1 hour.

1시간 경과후 연속냉각기(11)에 의해 47℃로 냉각한다.After 1 hour, it is cooled to 47 ° C by the continuous cooler (11).

침지액 발효탱크(4-1)~(4-3)에는 옥수수를 침지시킨 발효액 75l 유산활성균액 500l를 가하면 pH는 5~6정도이다. 이를 48시간 교반하면 유산발효가 이루어진다.To the immersion liquid fermentation tanks (4-1) to (4-3), the pH of the fermentation broth 75l lactic acid bacteria bacteria 500l is added, the pH is about 5-6. When this is stirred for 48 hours, lactic fermentation takes place.

이때 pH는 3~3.5로 되며, 발효는 자연히 적게되고 정지하며 액량 600l정도로 된다. 원료 침지조(1)(2)중 어디에 투입하는 경우도 침지액탱크(12)에 약 200l를 보내고 pH가 높은 공정수를 가해 pH4~4.5 정도로 조제하고 침지조에 필요량 투입한다.At this time, pH is 3 ~ 3.5, and fermentation is naturally reduced and stops, and the amount is about 600l. In the case of any of the raw material immersion tanks (1) and (2), about 200 liters are sent to the immersion liquid tank 12, process water having a high pH is added to prepare a pH of 4 to 4.5, and the required amount is added to the immersion tank.

이때 pH조절을 위해서 별도의 수산화나트륨(NaOH)을 첨가하여 pH를 5로 높여 조절할 수도 있다.At this time, by adding a separate sodium hydroxide (NaOH) to adjust the pH can be adjusted to increase the pH to 5.

공정수액탱크(13)에도 침지액 발효탱크(4-1)~(4-3)의 발효액을 보내고 공정수를 넣어 pH4~5로 해서 마쇄공정에 사용하면 침지곡립중의 당질의 발효에 도움(유산균의 증가)이 된다.The fermentation solution of the immersion liquid fermentation tanks (4-1) to (4-3) is also sent to the process solution tank (13), and the process water is added to the pH 4-5 to be used for the grinding process. Increase in lactic acid bacteria).

미설명부호 M은 밀링공정이고, M'는 밀링공정시 나오는 마쇄폐액(pH5-6)이다.Unexplained symbol M is the milling process, M 'is the grinding waste liquid (pH5-6) that comes out during the milling process.

Claims (1)

옥수수 부산물을 제조하기 위한 침지법에 있어서, 아황산에 의하여 침지하지 않고, 초기 침지시에는 3%소맥분 용액을 100℃, 3분 가열한 다음 47℃로 냉각하여 주박 및 소맥분에 의한 유산균으로 유산발효시켜 옥수수를 침지하고, 다음 침지시부터는 전분액(corn starch)을 고형분 2%로 희석한 액 500l당 별도의 소맥분 2.5~3㎏을 첨가하여 pH를 4~4.5로 한 원액을 100℃에서 6초간 130℃에서 1시간 가열한 다음 47℃로 냉각하여서 된 액 500l에, 1차 발효유산 활성균액 75l를 혼합하여 pH가 3~3.5로 되었을때의 교반발효액과 밀스타치 제조시의 마쇄폐액(pH5-6)을 혼합하여 pH를 5로 조정하여서 된 2차 발효유산 활성균액을 혼합하여 이에 옥수수를 침지시켜서 옥수수를 침지할 수 있게 함을 특징으로 하는 발효유산 활성균액에 의한 옥수수의 침지법.In the immersion method for producing corn by-products, without immersion by sulfurous acid, during the initial immersion, the 3% wheat flour solution is heated to 100 ° C. for 3 minutes and then cooled to 47 ° C. to lactic acid fermentation with lactic acid bacteria by sake and wheat flour. Soak corn, and from the next immersion, add 2.5 ~ 3kg of wheat flour per 500l of dilution of starch (solid starch) to 2% solids, and prepare the stock solution with pH 4 ~ 4.5 at 100 ℃ for 6 seconds. After stirring for 1 hour at 500 ° C., the solution was stirred at 47 ° C., and 75 l of the primary fermentation lactic acid active bacteria solution was mixed to obtain a pH of 3 to 3.5. ) Mixing the secondary fermented lactic acid active bacteria liquid by adjusting the pH to 5 by mixing) soaking the corn so as to soak the corn soaking the corn by fermented lactic acid active bacteria liquid, characterized in that.
KR1019870012489A 1987-11-06 1987-11-06 Dipping method of indian corn by fermentation lactobacillus KR900001424B1 (en)

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KR900001424B1 true KR900001424B1 (en) 1990-03-09

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