KR890701738A - 인체면역결핍 비루스 특이성 단백질분해효소와 이들의 합성 및 원형재현 방법 - Google Patents

인체면역결핍 비루스 특이성 단백질분해효소와 이들의 합성 및 원형재현 방법

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KR890701738A
KR890701738A KR1019890700204A KR890700204A KR890701738A KR 890701738 A KR890701738 A KR 890701738A KR 1019890700204 A KR1019890700204 A KR 1019890700204A KR 890700204 A KR890700204 A KR 890700204A KR 890701738 A KR890701738 A KR 890701738A
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protease
amino acid
human immunodeficiency
proteolytic enzyme
acid sequence
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KR1019890700204A
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스테펜 오로슬랜
테리 디. 코포랜드
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원본미기재
바이오네틱스 리서치 인코오포레이티드
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Publication of KR890701738A publication Critical patent/KR890701738A/ko

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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/503—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from viruses
    • C12N9/506—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from viruses derived from RNA viruses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents

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Abstract

내용 없음

Description

인체면역결핍 비루스 특이성 단백질분해효소와 이들의 합성 및 원형재현 방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 본 발명의 프로테아제를 비롯한 레트로 비루스성 프로테아제의 아미노산 보존서열을 예시하는 것이다. 점들은 동일 잔기 또는 보존성치환체를 표시한다. 제2도는 HIV-1(BH10)프로테아제의 아미노산 서열이며, HIV-2 프로테아제에서는 비균일 서열이 발견되었다.

Claims (11)

  1. gag 및 gag-pol 전구체 다단백질을 분해하여 인체면역결핍 비루스를 복제하는데 필요한 단백질분해활성을 가지는 단백질 분해효소.
  2. 제1항에 있어서, HIV-1의 전구체다단백질을 분해시킬 수 있는 단백질분해효소.
  3. 제2항에 있어서, 하기의 아미노산서열로 구성되는 단백질분해효소:ProGlnIleThrLeuTrpGlnArgProLeuValThrIleLysIleGlyGlyGlnLeuLysGluAlaLeuLeuAspThrGlyAlaAspAspThrValLeuGluGlMetSerLeuProGlyArgTrpLysProLysMetIleGlyGlyIleGlyGlyPheIleLysValArgGlnTyrAsPGlnIleLeuIleGluIleCysGluIleCysClyHisLysAlaIleGlyThrValLeuValGlyProThrProValAsnIleIleGlyArgAsnLeuLeuThrGlnIleGlyCysThrLeuAsnPhe.
  4. 제2항에 있어서, 하기의 아미노산서열로 구성되는 단백질분해효소:ProGlnIleThrLeuTrpGlnArgP.roLeuValThrIleLysIleGlyGlyGlnLeuLysGluAlaLeuLeuAspThrGlyAlaAspAspThrValLeuGluGlMetSerLeuProGlyArgTrpLysProLysMetIleGlyGlyIleGlyGlyPheIleLysValArgGlnTyrAsPGlnIleLeuIleGluIleCysGluIleCysClyHisLysAlaIleGlyThrValLeuValGlyProThrProValAsnIleIleGlyArgAsnLeuLeuThrGlnIleGlyCysThrLeuAsnPhe.
  5. 제1항에 있어서, HIV-2의 전구체 다단백질을 분해시킬 수 있는 단백질분해효소.
  6. 제4항에 있어서, 하기의 아미노산서열로 구성되는 단백질분해효소:ProGlnPheSerLeuTrpLysArgProValValThrAlaTyrIleGluGlyGlnProValGluValLeuLeuAspThrGlyAlaAspAspSerIleValAlaGlyIleGluLeuGlyuLeuGlyAsnAsnTyrSerProLysIleValGlyGlyIleGlyGlyPheIleAsnThrLysGluTyrLysAsnValGluIleGluValLeuAsnLysLysValArgAlaThrIleMetThrGlyAspT.hrProIleAsnIlePheGlyArgAsnIleLeuThrAlaLeuGlyMetSerLeuAsnLeu.
  7. 제4항에 있어서, 하기의 아미노산서열로 구성되는 단백질분해효소:ProGlnPheSerLeuTrpLysArgProValValThrAlaTyrIleGluGlyGlnProValGluValLeuLeuAspThrGlyAlaAspAspSerIleValAlaGlyIleGluLeuGlyuLeuGlyAsnAsnTyrSerProLysIleValGlyGlyIleGlyGlyPheIleAsnThrLysGluTyrLysAsnValGluIleGluValLeuAsnLysLysValArgAlaThrIleMetThrGlyAspT.hrProIleAsnIlePheGlyArgAsnIleLeuThrAlaLeuGlyMetSerLeuAsnLeu.
  8. 제3항에 있어서, 합성법에 의해 단백질분해활성을 제공하는 입체특이성 구조로 제조된 단백질분해효소.
  9. 제5항에 있어서, 합성법에 의해 단백질분해활성을 제공하는 입체특이성 구조로 제조된 단백질분해효소.
  10. 제3항의 단백질분해효소를 코오딩하는 DNA 서열.
  11. 제5항의 단백질분해효소를 코오딩하는 DNA 서열.
    ※ 참고사항：최초출원 내용에 의하여 공개하는 것임.
KR1019890700204A 1987-06-01 1988-06-01 인체면역결핍 비루스 특이성 단백질분해효소와 이들의 합성 및 원형재현 방법 KR890701738A (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US057,183 1987-06-01
US07/057,183 US5252477A (en) 1987-06-01 1987-06-01 Human immunodeficiency virus specific proteolytic enzyme and a method for its synthesis and renaturation
PCT/US1988/001849 WO1988009815A1 (en) 1987-06-01 1988-06-01 Human immunodeficiency virus specific proteolytic enzyme and a method for its synthesis and renaturation

Publications (1)

Publication Number Publication Date
KR890701738A true KR890701738A (ko) 1989-12-21

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KR1019890700204A KR890701738A (ko) 1987-06-01 1988-06-01 인체면역결핍 비루스 특이성 단백질분해효소와 이들의 합성 및 원형재현 방법

Country Status (10)

Country Link
US (2) US5252477A (ko)
EP (1) EP0316429B1 (ko)
JP (1) JPH02500408A (ko)
KR (1) KR890701738A (ko)
AT (1) ATE105859T1 (ko)
AU (1) AU630590B2 (ko)
DE (1) DE3889625T2 (ko)
DK (1) DK41289A (ko)
FI (1) FI890459A0 (ko)
WO (1) WO1988009815A1 (ko)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5637488A (en) * 1987-06-01 1997-06-10 The United States Of America As Represented By The Department Of Health And Human Services HIV protease gene and method for its expression
US5252477A (en) * 1987-06-01 1993-10-12 The United States Of America As Represented By The United States Department Of Health And Human Services Human immunodeficiency virus specific proteolytic enzyme and a method for its synthesis and renaturation
CA1340748C (en) * 1988-07-13 1999-09-14 Stephen Oroszlan Synthetic hiv protease gene and method for its expression
US5147369A (en) * 1991-07-01 1992-09-15 Wagner Michael A Forceps and method for nuclear fragment removal
US5554728A (en) * 1991-07-23 1996-09-10 Nexstar Pharmaceuticals, Inc. Lipid conjugates of therapeutic peptides and protease inhibitors
RU2126794C1 (ru) * 1992-03-11 1999-02-27 Нархекс Лимитед Аминопроизводные оксо- или гидроксизамещенных гидразинов, способ их получения и фармацевтические композиции для ингибирования ретровирусной протеазы
US5888992A (en) * 1992-03-11 1999-03-30 Narhex Limited Polar substituted hydrocarbons
ATE253050T1 (de) 1992-03-11 2003-11-15 Narhex Ltd Aminderivate von oxo- und hydroxy- substituierten kohlenwasserstoffen
US6071895A (en) * 1992-03-11 2000-06-06 Narhex Limited Polar-substituted hydrocarbons
US5872210A (en) * 1995-10-05 1999-02-16 The United States Of America As Represented By The Department Of Health And Human Services Transframe peptide inhibitor of viral protease
EP0909322B1 (en) 1996-04-30 2005-11-16 Twinstrand Therapeutics Inc. Antiviral ricin-like proteins
US6531125B1 (en) 1999-03-02 2003-03-11 Twinstrand Therapeutics Inc. Antiviral ricin-like proteins

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5252477A (en) * 1987-06-01 1993-10-12 The United States Of America As Represented By The United States Department Of Health And Human Services Human immunodeficiency virus specific proteolytic enzyme and a method for its synthesis and renaturation

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AU630590B2 (en) 1992-11-05
FI890459A (fi) 1989-01-31
US5252477A (en) 1993-10-12
US5354683A (en) 1994-10-11
FI890459A0 (fi) 1989-01-31
EP0316429A1 (en) 1989-05-24
EP0316429B1 (en) 1994-05-18
DK41289D0 (da) 1989-01-30
EP0316429A4 (en) 1991-05-22
AU1805188A (en) 1989-01-04
WO1988009815A1 (en) 1988-12-15
DE3889625T2 (de) 1994-09-29
JPH02500408A (ja) 1990-02-15
DE3889625D1 (de) 1994-06-23
ATE105859T1 (de) 1994-06-15
DK41289A (da) 1989-01-30

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