KR890005271A - 스트렙토마이신- 민감성 유전자를 함유하는 플라즈미드 벡터 - Google Patents

스트렙토마이신- 민감성 유전자를 함유하는 플라즈미드 벡터 Download PDF

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KR890005271A
KR890005271A KR1019880012513A KR880012513A KR890005271A KR 890005271 A KR890005271 A KR 890005271A KR 1019880012513 A KR1019880012513 A KR 1019880012513A KR 880012513 A KR880012513 A KR 880012513A KR 890005271 A KR890005271 A KR 890005271A
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dna fragment
plasmid vector
streptomycin
phsg
gene
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KR1019880012513A
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마리 토바
모또 다모쭈 하지
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아다찌 가쯔오
훽스트 제팬 리미티드
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Publication of KR890005271A publication Critical patent/KR890005271A/ko

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C12N15/68—Stabilisation of the vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C12N15/71—Expression systems using regulatory sequences derived from the trp-operon

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  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
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Abstract

내용 없음

Description

스트렙토마이신-민감성 유전자를 함유하는 플라즈미드 벡터
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 플라즈미드 pHSG 673에 대한 제한효소 절단 지도이다.
제2도는 플라즈미드 pHSG 673에 대한 제한효소 절단 지도이다.

Claims (8)

  1. 암호화 영역의 필수 부위에 넌센스(nonsense)코돈 하나 이상을 갖는 스트렙토마이신-민감성 유전자를 함유하는 DNA 단편.
  2. 제1항에 있어서, 유도성 프로모터가, 상기의 스트렙토마이신-민감성 유전자의 발현을 조절할 수 있도록 상기 유전자의 상부에 위치하는 DNA 단편.
  3. 제2항에 있어서, 암호화 영역내의 넌센스 코돈이 엠버 코돈인 DNA 단편.
  4. 제2항에 있어서, 유도성 프로모터가 트립토판 프로모터인 DNA 단편.
  5. 제2항에 있어서, 표 1에 나타낸 뉴클레오타이드 서열을 지니며, 암호화 영역내의 넌센스 코돈이 앰버코돈이고, 유도성 프로모터가 트립토판 프로모터의 DNA 단편.
  6. 암호와 영역내에 넌센스 코돈을 지니는 스트렙토마이신-민감성 유전자 및 상기 유전자의 발현 조절할 수 있도록 이의 상부에 존재하는 유도성 프로모터를 동반하는 플라즈미드 벡터민 pHSG 673 및 pHSG 683으로 구성된 그룹으로부터 선택되는 플라즈미드 벡터.
  7. 양호와 영역내에 넌센스 코돈을 지니는 스트렙토마이신-민감성 유전자 및 상기 유전자의 발현을 조절할 수 있도록 이의 상부에 존재하는 유도성 프로모터를 동반하는 플라즈미드 벡터인 pHSG 673 및 pHSG 683으로 구성된 그룹으로부터 선택되는 플라즈미드 벡터를 함유하는 미생물.
  8. 제7항에 있어서, 미생물이 에스케리챠 콜라이(Escherichia coli)인 미생물.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019880012513A 1987-09-29 1988-09-28 스트렙토마이신- 민감성 유전자를 함유하는 플라즈미드 벡터 KR890005271A (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP62245042A JPS6486875A (en) 1987-09-29 1987-09-29 Plasmid vector containing streptomycin-sensitive gene
JP62-245042 1987-09-29

Publications (1)

Publication Number Publication Date
KR890005271A true KR890005271A (ko) 1989-05-13

Family

ID=17127714

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019880012513A KR890005271A (ko) 1987-09-29 1988-09-28 스트렙토마이신- 민감성 유전자를 함유하는 플라즈미드 벡터

Country Status (7)

Country Link
EP (1) EP0309961A1 (ko)
JP (1) JPS6486875A (ko)
KR (1) KR890005271A (ko)
AU (1) AU2290588A (ko)
DK (1) DK538988A (ko)
FI (1) FI884420A (ko)
PT (1) PT88606A (ko)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5691185A (en) * 1993-10-08 1997-11-25 Chr. Hansen A/S Lactic acid bacterial suppressor mutants and their use as selective markers and as means of containment in lactic acid bacteria

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0223156B1 (en) * 1985-11-12 1991-05-22 Hoechst Japan Limited Streptomycin sensitive gene having a lot of cloning sites, plasmid vector containing the same and microorganism containing the vector

Also Published As

Publication number Publication date
AU2290588A (en) 1989-04-06
DK538988D0 (da) 1988-09-28
FI884420A (fi) 1989-03-30
FI884420A0 (fi) 1988-09-27
EP0309961A1 (en) 1989-04-05
DK538988A (da) 1989-03-30
JPS6486875A (en) 1989-03-31
PT88606A (pt) 1989-07-31

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