KR890000669A - L-트리프토판의 제조법 - Google Patents

L-트리프토판의 제조법 Download PDF

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KR890000669A
KR890000669A KR1019880007195A KR880007195A KR890000669A KR 890000669 A KR890000669 A KR 890000669A KR 1019880007195 A KR1019880007195 A KR 1019880007195A KR 880007195 A KR880007195 A KR 880007195A KR 890000669 A KR890000669 A KR 890000669A
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ferm
concentration
microorganism
malic acid
reaction system
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KR1019880007195A
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히사시 야마가따
후지오 엔도오
미쓰노부 시마쓰
마사또 데라사와
히데아끼 고까와
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노구찌 데루오
게이시쯔류분 신요오또 가이하쓰 기쥬쓰껭뀨 꾸미아이
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Priority claimed from JP14697287A external-priority patent/JPH0824591B2/ja
Priority claimed from JP18123787A external-priority patent/JPS6427486A/ja
Priority claimed from JP28752587A external-priority patent/JPH01128792A/ja
Application filed by 노구찌 데루오, 게이시쯔류분 신요오또 가이하쓰 기쥬쓰껭뀨 꾸미아이 filed Critical 노구찌 데루오
Publication of KR890000669A publication Critical patent/KR890000669A/ko

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/22—Tryptophan; Tyrosine; Phenylalanine; 3,4-Dihydroxyphenylalanine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/22—Tryptophan; Tyrosine; Phenylalanine; 3,4-Dihydroxyphenylalanine
    • C12P13/227—Tryptophan
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
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  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

내용 없음

Description

L-트리프토판의 제조법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음

Claims (18)

  1. (A) 푸마라아제, (B) 피루브산-말산 카르복실라아제, (C) 트리프토파나아제 및 (D) NADH 옥시다아제의 존재하에 푸마르산 또는 이의 염, 암모늄이온 및 5-위치에서 히드록실기로 치환될 수 있는 인들을 반응시킴을 특징으로 하는, 5-위치에서 히드록실기로 치환될 수 있는 L-트리프토판의 제조방법.
  2. 제1항에 있어서, 푸마라아제(A) 및 피루브산-말산 카르복실라아제 (B)를 동시에 생산하는 능력을 지닌 브레비박테리움(Brevibacterium) 속의 미생물 또는 이의 처리물이 푸마라아제 (A) 및 피루브산-말산 카르복실라아제 (B)로 사용되는 방법.
  3. 제2항에 있어서, 미생물 세포가 브레비박테리움 플라붐(Brevibacterium flavum) MJ-223(FERM BP-1497) 또는 브레비박테리움 플라붐 MJ-223-AB-41(FERM BP-1498)인 방법.
  4. 제1항에 있어서, 푸마라아제(A), 피루브산-말산 카르복실라아제(B) 및 트리프토나아제(C)를 생산하는 능력을 지닌 에스케리키아(Escherichia) 속, 프로테우스(Proteus) 속 또는 에르위니아(Erwinia)속의 미생물 세포, 또는 이의 처리물이 상기 효소(A), (B) 및 (C)로 사용되는 방법.
  5. 제4항에 있어서, 미생물 세포가 에스케리키아 콜리(Escherichia coli) K-12 균주(ATCC 27325, FERM BP-1733) 또는 FERM BP-1734), 프로테우스 모르가니이(Proteusmorganii) (IFO-3848) 또는 에르위니아 해르비콜라(Erwinia herbicola) (ATCC 21433)인 방법.
  6. 제1항에 있어서, 피루브산-말산 카르복실라아제(B) 및 트리프토나아제(C)가 적어도 이들 두가지 효소들을 동시에 생산하는 능력을 지닌 미생물을 말산 또는 이의 염을 주 탄소원으로 함유하는 배지중에서 배양시켜 수득한 미생물 세포 또는 이의 처리물인 방법.
  7. 제6항에 있어서, 미생물이 적어도 트리프토파나아제의 구조 유전자를 함유하는 플라스미드로 형질전환된 에스케리키아 콜리종에 속하는 트리프토하나아제-생산 미생물인 방법.
  8. 제7항에 있어서, 미생물이 에스케리키아 콜리 K-12 YK 3002(FERM BP-1733), 에스케리키아 콜리 K-12 YK 3003 (FERM BP-1734) 또는 에스케리키아 콜리 K-12 YK 3004(FERM BP-1735)인 방법.
  9. 제1항에 있어서, NADH 옥시다이제 (D)가 로이코노스톡 메센테로이데스(Leuconostoc mesenter-oides) (ATCC 9135), 바실루스 세레우스(Bacillus cereus) (ATCC 4342) 또는 이의 처리물인 방법.
  10. 제1항에 있어서, 반응계중의 푸마르산 또는 이의 염 및 암모늄 이온의 농도가 0.1∼20％(중량/용적)인 방법.
  11. 제1항에 있어서, 반응계중의 5-위치에서 히드록실기로 치환될수 있는 인돌의 농도가 0.1∼20％(중량/용적)인 방법.
  12. 제1항에 있어서, 인돌이 이의 평균 농도가 4mM을 넘지 않도록 조절된 조건하에서 연속적으로 또는 간헐적으로 첨가되는 방법.
  13. 제1항에 있어서, 반응이 환원제의 존재하에 진행되는 방법.
  14. 제13항에 있어서, 환원제가 디티오트레이톨, 디티오 에리트리톨, 2-메르캅토에탄올, L-시스테인, L-아스코르브산 및 아황산나트륨중에서 선택되는 방법.
  15. 제13항에 있어서, 환원제의 농도가 0.0001∼10％(중량/용적)인 방법.
  16. 제1항에 있어서, 산화형 또는 환원형 니코틴아미드아데닌 디뉴클레오티드(NAD+또는 NADH) 및/또는 금속이온이 반응계에 첨가되는 방법.
  17. 제16항에 있어서, 반응계중의 NAD+또는 NADH의 농도가 0.005∼20mM인 방법.
  18. 제16항에 있어서, 반응계중의 금속 이온의 농도가 0.1∼20mM인 방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019880007195A 1987-06-15 1988-06-15 L-트리프토판의 제조법 KR890000669A (ko)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
JP146,972/87 1987-06-15
JP14697287A JPH0824591B2 (ja) 1987-06-15 1987-06-15 Ｌ−トリプトフアン類の製造法
JP18123787A JPS6427486A (en) 1987-07-22 1987-07-22 Production of l-tryptophan
JP181,237/87 1987-07-22
JP287,525/87 1987-11-16
JP28752587A JPH01128792A (ja) 1987-11-16 1987-11-16 Ｌ−トリプトフアンの製造法

Publications (1)

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KR890000669A true KR890000669A (ko) 1989-03-16

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KR1019880007195A KR890000669A (ko) 1987-06-15 1988-06-15 L-트리프토판의 제조법

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EP (1) EP0295622B1 (ko)
KR (1) KR890000669A (ko)
DE (1) DE3884468T2 (ko)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0385415B1 (en) * 1989-02-28 1995-06-07 Mitsubishi Petrochemical Co., Ltd. Process for producing NADH oxidase
DE4427486C2 (de) * 1994-08-03 2003-03-27 Dev Ct For Biotechnology Taipe Verfahren zur enzymatischen Synthese von L-Tryptophan mit einem computergesteuerten Bioreaktorsystem

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS4946917B1 (ko) * 1970-12-22 1974-12-12
WO1984000383A1 (en) * 1982-07-17 1984-02-02 Grand Metropolitan Biotech Enzymatic reaction process

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DE3884468T2 (de) 1994-02-24
DE3884468D1 (de) 1993-11-04
EP0295622B1 (en) 1993-09-29
EP0295622A2 (en) 1988-12-21
EP0295622A3 (en) 1990-01-10

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