KR840001215A - 개량된 변종균주의 제법 - Google Patents

개량된 변종균주의 제법 Download PDF

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Publication number
KR840001215A
KR840001215A KR1019820003543A KR820003543A KR840001215A KR 840001215 A KR840001215 A KR 840001215A KR 1019820003543 A KR1019820003543 A KR 1019820003543A KR 820003543 A KR820003543 A KR 820003543A KR 840001215 A KR840001215 A KR 840001215A
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South Korea
Prior art keywords
strain
bordetella bronchiseptica
sensitive
cells
temperature
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KR1019820003543A
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English (en)
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다께시 시미즈
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로꾸단다 도끼찌 (외 2)
가가꾸 오요 겟세이 료호겐꾸쇼
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Publication of KR840001215A publication Critical patent/KR840001215A/ko

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/01—Preparation of mutants without inserting foreign genetic material therein; Screening processes therefor
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/02—Bacterial antigens
    • A61K39/099—Bordetella
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
    • A61K2039/52—Bacterial cells; Fungal cells; Protozoal cells
    • A61K2039/522—Bacterial cells; Fungal cells; Protozoal cells avirulent or attenuated
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S424/00—Drug, bio-affecting and body treating compositions
    • Y10S424/825—Bacterial vaccine for porcine species, e.g. swine
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S424/00—Drug, bio-affecting and body treating compositions
    • Y10S424/83—Drug, bio-affecting and body treating compositions involving temperature-sensitive mutant bacterium

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Mycology (AREA)
  • Physics & Mathematics (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Cell Biology (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

내용 없음

Description

개량된 변종균주의 제법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 본 발명에 따른 균락증식율을 나타낸 도면이다.

Claims (1)

  1. (1)보르데텔라 브론키셉티카(Bordetella Bronchiseptica)의 균주에 변종원을 접종시켜 적어도 온도-민감성(32℃에서 증식할 수 있지만 34℃ 및 그 이상에서 증식할 수 없음) 및 우레아제 음성인 유전적 마-커를 갖는 변종균주를 얻고, 이 변종균주를 피-한천배지에서 배양하여 비교적 작은 균락으로 증식된 세포를 동정분리한 후 이 세포를 피-한천배지에서 배양하여 세포의 동정분리과정을 반복함으로서 배양물을 완전히 캡슐형 항원을 가지며 용혈성인 페이스I 유기체로 전환시킴을 특징으로 하는보르데텔라 브론키셉티카감염의 예방용 생백신의 제조에 유용한 적어도 온도-민감성이며 우레아제 음성인 다수의 유전적 염색체 마-커를 가진보르데텔라 브론키셉티카의 변종균주를 제조하는 방법.
    (2) 제1항에 있어서 변종원이 나니트로소구아니딘과 자외선 조사의 처리의 배합임을 특징으로 하는 방법.
    (3) 제1항에 있어서 세포의 동정분리의 과정과 배양을 2회 또는 그 이상 반복함을 특징으로 하는 방법.
    (4) 제3항에 있어서 세포의 분리동정과 배양을 2회 내지 10회 반복함을 특징으로 하는 방법.
    (5) 제1항에 있어서보르데텔라 브론키셉티카의 출발균주가 온도-민감성을 가진 변종균주임을 특징으로 하는 방법.
    (6) 제1항에 있어서보르데텔라 브론키셉티카의 출발균주가 야생균주임을 특징으로 하는 방법.
    (7) 적어도 온도-민감성(32℃에서 증식되지만 34℃, 및 그 이상에서 증식되지 않음)의 유전적 염색체 마-커 및 우레아제 음성을 가진보르데텔라 브론키셉티카의 페이스I 균주 ts-S34.u-로 형성됨을 특징으로 하는보르데텔라 브론키셉티카감염의 예방용 생약독백신.
    (8) 제7항에 있어서 백신이 건조보호제와 함께 냉동건조된 형태임을 특징으로 하는 생약독백신.
    (9) 적어도 온도-민감성(32℃에서 증식되지만 34℃ 및 그 이상에서 증식되지 않음) 및 동물에 대해 우레아제 음성을 가진 유전적 염색체 마-커를 가진보르데텔라 브론키셉티카의 페이스I균주 ts-S34.u-로 제조된 생약독백신을 투여함을 특징으로 하는보르데텔라 브론키셉티카감염의 예방법.
    (10) 제9항에 있어서 생약독백신을 동물의 비강을 통해서 동물에 투여함을 특징으로 하는 방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019820003543A 1981-08-10 1982-08-06 개량된 변종균주의 제법 KR840001215A (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP125530 1981-08-10
JP56125530A JPS5953828B2 (ja) 1981-08-10 1981-08-10 ボルデテラ・プロンヒセブティカ感染症生菌ワクチン用改良原株の作出法および生菌ワクチン

Publications (1)

Publication Number Publication Date
KR840001215A true KR840001215A (ko) 1984-03-28

Family

ID=14912457

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019820003543A KR840001215A (ko) 1981-08-10 1982-08-06 개량된 변종균주의 제법

Country Status (8)

Country Link
US (1) US4456588A (ko)
EP (1) EP0072656B1 (ko)
JP (1) JPS5953828B2 (ko)
KR (1) KR840001215A (ko)
AT (1) ATE18135T1 (ko)
CA (1) CA1185549A (ko)
DE (1) DE3269388D1 (ko)
DK (1) DK169707B1 (ko)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4888169A (en) * 1986-10-14 1989-12-19 Norden Laboratories, Inc. Bordetella Bronchiseptica vaccine
JP2537042B2 (ja) * 1986-11-27 1996-09-25 社団法人北里研究所 ボルデテラ・ブロンキセプチカ感染症生菌ワクチン用原株の製造法および生菌ワクチン
JPH01122825U (ko) * 1988-02-04 1989-08-21
US20050026866A1 (en) * 2002-08-02 2005-02-03 Pawelek John M. Agents and methods for treatment of disease by oligosaccharide targeting agents
EP2461927A4 (en) * 2009-08-09 2017-05-17 Rolls-Royce Corporation System, method, and apparatus for pouring casting material in an investment cast

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE598680C (de) * 1930-12-24 1934-06-15 Fritz Kirstein Dr Verfahren und Vorrichtung zur Herstellung von Vaccinen aus Bakterien
DE600941C (de) * 1931-05-19 1934-08-03 Fritz Kirstein Dr Verfahren zur Herstellung von Vaccinen
US4225583A (en) * 1978-12-07 1980-09-30 Iowa State University Research Foundation, Inc. Intra-respiratory vaccine for prevention of Bordetella bronchiseptica infection and method of use

Also Published As

Publication number Publication date
US4456588A (en) 1984-06-26
EP0072656B1 (en) 1986-02-26
JPS5828276A (ja) 1983-02-19
EP0072656A1 (en) 1983-02-23
DK349782A (da) 1983-02-11
DK169707B1 (da) 1995-01-16
CA1185549A (en) 1985-04-16
DE3269388D1 (en) 1986-04-03
JPS5953828B2 (ja) 1984-12-27
ATE18135T1 (de) 1986-03-15

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