KR20240145084A - 조작되고 완전-기능 맞춤 당단백질 - Google Patents

조작되고 완전-기능 맞춤 당단백질 Download PDF

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Publication number
KR20240145084A
KR20240145084A KR1020247032085A KR20247032085A KR20240145084A KR 20240145084 A KR20240145084 A KR 20240145084A KR 1020247032085 A KR1020247032085 A KR 1020247032085A KR 20247032085 A KR20247032085 A KR 20247032085A KR 20240145084 A KR20240145084 A KR 20240145084A
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KR
South Korea
Prior art keywords
sequence
host cell
target protein
leishmania
sialyltransferase
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Pending
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KR1020247032085A
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English (en)
Korean (ko)
Inventor
아밀레자 파리드모아이어
마누엘라 말리
라이너 폴라도르
Original Assignee
림마테크 바이오로직스 아게
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Publication of KR20240145084A publication Critical patent/KR20240145084A/ko
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/44—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from protozoa
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/10—Protozoa; Culture media therefor
    • C12N1/105—Protozoal isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1081—Glycosyltransferases (2.4) transferring other glycosyl groups (2.4.99)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/40—Immunoglobulins specific features characterized by post-translational modification
    • C07K2317/41—Glycosylation, sialylation, or fucosylation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/04—Fusion polypeptide containing a localisation/targetting motif containing an ER retention signal such as a C-terminal HDEL motif
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/05—Fusion polypeptide containing a localisation/targetting motif containing a GOLGI retention signal
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/30—Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/90—Protozoa ; Processes using protozoa
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/01003—Glucosamine N-acetyltransferase (2.3.1.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01022—Lactose synthase (2.4.1.22)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01101—Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase (2.4.1.101)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01143—Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase (2.4.1.143)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/99—Glycosyltransferases (2.4) transferring other glycosyl groups (2.4.99)
    • C12Y204/99006—N-Acetyllactosaminide alpha-2,3-sialyltransferase (2.4.99.6)

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
KR1020247032085A 2017-06-30 2018-06-28 조작되고 완전-기능 맞춤 당단백질 Pending KR20240145084A (ko)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201762527466P 2017-06-30 2017-06-30
US62/527,466 2017-06-30
PCT/EP2018/067494 WO2019002512A2 (en) 2017-06-30 2018-06-28 GLYCOPROTEINS CUSTOMIZED FROM ENGINEERING AND FULLY FUNCTIONAL
KR1020207002825A KR102711998B1 (ko) 2017-06-30 2018-06-28 조작되고 완전-기능 맞춤 당단백질

Related Parent Applications (1)

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KR1020207002825A Division KR102711998B1 (ko) 2017-06-30 2018-06-28 조작되고 완전-기능 맞춤 당단백질

Publications (1)

Publication Number Publication Date
KR20240145084A true KR20240145084A (ko) 2024-10-04

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Family Applications (2)

Application Number Title Priority Date Filing Date
KR1020247032085A Pending KR20240145084A (ko) 2017-06-30 2018-06-28 조작되고 완전-기능 맞춤 당단백질
KR1020207002825A Active KR102711998B1 (ko) 2017-06-30 2018-06-28 조작되고 완전-기능 맞춤 당단백질

Family Applications After (1)

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KR1020207002825A Active KR102711998B1 (ko) 2017-06-30 2018-06-28 조작되고 완전-기능 맞춤 당단백질

Country Status (7)

Country Link
US (2) US11913051B2 (https=)
EP (1) EP3645732A2 (https=)
JP (3) JP7437162B2 (https=)
KR (2) KR20240145084A (https=)
CN (3) CN119639577A (https=)
AU (1) AU2018294504B2 (https=)
WO (1) WO2019002512A2 (https=)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20240145084A (ko) 2017-06-30 2024-10-04 림마테크 바이오로직스 아게 조작되고 완전-기능 맞춤 당단백질
WO2019234021A1 (en) 2018-06-04 2019-12-12 Limmatech Biologics Ag Glycoengineered monoclonal antibody
CN111518736B (zh) 2019-10-24 2022-04-15 华熙生物科技股份有限公司 一种高效合成高纯度透明质酸及其寡聚糖的重组谷氨酸棒状杆菌
WO2021140144A1 (en) 2020-01-07 2021-07-15 Limmatech Biologics Ag Engineered leishmania cells
JP7684310B2 (ja) * 2020-01-07 2025-05-27 リンマテック バイオロジクス エージー Ｌｅｉｓｈｍａｎｉａ細胞を使用する糖鎖修飾改変
EP4161645A4 (en) * 2020-06-08 2023-11-01 Yale University COMPOSITIONS AND METHODS FOR TREATING AND/OR PREVENTING COAGULOPATHY AND/OR SEPSIS IN PATIENTS WITH BACTERIAL AND/OR VIRAL INFECTIONS
WO2022053673A1 (en) 2020-09-14 2022-03-17 Limmatech Biologics Ag Fab sialylation of antibodies
WO2022200390A2 (en) 2021-03-23 2022-09-29 Glycoera Ag Glycan-mediated protein degradation
JP2024513755A (ja) 2021-03-23 2024-03-27 グリコエラ アーゲー マンノース３グリカン媒介タンパク質分解
JP2024517711A (ja) * 2021-04-29 2024-04-23 ノバルティス アーゲー 高シアル酸付加細胞
WO2024068753A1 (en) 2022-09-28 2024-04-04 Glycoera Ag Glycan-mediated protein degradation
WO2024147113A1 (en) 2023-01-05 2024-07-11 Glycoera Ag Glycoengineered polypeptides targeting anti-podocyte autoantibodies and uses thereof
JP2026501773A (ja) 2023-01-05 2026-01-16 グリコエラ アーゲー 免疫グロブリンａを標的とする糖鎖改変ポリペプチド及びそれを含む複合体
US20260055211A1 (en) 2023-01-05 2026-02-26 Glycoera Ag Glycoengineered polypeptides targeting anti-neutrophil autoantibodies and uses thereof
WO2024218743A1 (en) 2023-04-21 2024-10-24 Glycoera Ag Multi-functional molecules comprising glycans and uses thereof
WO2025215516A1 (en) 2024-04-08 2025-10-16 Glycoera Ag Glycoengineered polypeptides targeting igg4 autoantibodies and uses thereof
TW202602920A (zh) * 2024-04-26 2026-01-16 磐石醫藥生技股份有限公司 工程化之醣蛋白群體及其用途

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WO2001032896A1 (en) * 1999-11-05 2001-05-10 Jena Bioscience Gmbh Protein expression systems for non-pathogenic <i>kinetoplastidae</i>
US7863020B2 (en) * 2000-06-28 2011-01-04 Glycofi, Inc. Production of sialylated N-glycans in lower eukaryotes
CN107119095B (zh) * 2008-01-03 2022-07-05 康乃尔研究基金会有限公司 原核生物中的糖基化蛋白表达
JP5652206B2 (ja) 2008-10-01 2015-01-14 旭硝子株式会社 宿主、形質転換体およびその製造方法、ならびにｏ−グリコシド型糖鎖含有異種蛋白質の製造方法
AU2012329090A1 (en) * 2011-10-28 2014-05-01 Merck Sharp & Dohme Corp. Methods for increasing N-glycan occupancy and reducing production of hybrid N-glycans in Pichia pastoris strains lacking Alg3 expression
CA3006400A1 (en) 2015-11-30 2017-06-08 Limmatech Biologics Ag Methods of producing glycosylated proteins
KR20240145084A (ko) 2017-06-30 2024-10-04 림마테크 바이오로직스 아게 조작되고 완전-기능 맞춤 당단백질
JP7684310B2 (ja) 2020-01-07 2025-05-27 リンマテック バイオロジクス エージー Ｌｅｉｓｈｍａｎｉａ細胞を使用する糖鎖修飾改変
WO2021140144A1 (en) 2020-01-07 2021-07-15 Limmatech Biologics Ag Engineered leishmania cells
WO2022053673A1 (en) 2020-09-14 2022-03-17 Limmatech Biologics Ag Fab sialylation of antibodies

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Publication number Publication date
CN111108211A (zh) 2020-05-05
JP2023134519A (ja) 2023-09-27
WO2019002512A2 (en) 2019-01-03
AU2018294504A1 (en) 2020-01-23
JP2020530273A (ja) 2020-10-22
JP7437162B2 (ja) 2024-02-22
CN117487873A (zh) 2024-02-02
KR102711998B1 (ko) 2024-10-04
WO2019002512A3 (en) 2019-02-28
KR20200022486A (ko) 2020-03-03
CN119639577A (zh) 2025-03-18
US11913051B2 (en) 2024-02-27
EP3645732A2 (en) 2020-05-06
US20240287568A1 (en) 2024-08-29
AU2018294504B2 (en) 2024-07-11
JP2026067872A (ja) 2026-04-21
US20210332403A1 (en) 2021-10-28

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