KR20240135608A - 조작된 고활성 omni-79 뉴클레아제 변이체 - Google Patents

조작된 고활성 omni-79 뉴클레아제 변이체 Download PDF

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Publication number
KR20240135608A
KR20240135608A KR1020247021908A KR20247021908A KR20240135608A KR 20240135608 A KR20240135608 A KR 20240135608A KR 1020247021908 A KR1020247021908 A KR 1020247021908A KR 20247021908 A KR20247021908 A KR 20247021908A KR 20240135608 A KR20240135608 A KR 20240135608A
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South Korea
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omni
amino acid
nuclease
cell
cells
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KR1020247021908A
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Korean (ko)
Inventor
리오르 이자르
라피 엠마누엘
리아트 록카
다비드 밀릿 마롬
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에멘도바이오 인코포레이티드
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Publication of KR20240135608A publication Critical patent/KR20240135608A/ko
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    • C—CHEMISTRY; METALLURGY
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    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
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    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
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    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
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    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C40B40/08—Libraries containing RNA or DNA which encodes proteins, e.g. gene libraries
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/09—Fusion polypeptide containing a localisation/targetting motif containing a nuclear localisation signal
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
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    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14141—Use of virus, viral particle or viral elements as a vector
    • C12N2750/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector

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  • General Engineering & Computer Science (AREA)
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  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
KR1020247021908A 2021-12-01 2022-11-30 조작된 고활성 omni-79 뉴클레아제 변이체 Pending KR20240135608A (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202163284858P 2021-12-01 2021-12-01
US63/284,858 2021-12-01
PCT/US2022/080627 WO2023102407A2 (en) 2021-12-01 2022-11-30 Engineered high activity omni-79 nuclease variants

Publications (1)

Publication Number Publication Date
KR20240135608A true KR20240135608A (ko) 2024-09-11

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ID=86613099

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KR1020247021908A Pending KR20240135608A (ko) 2021-12-01 2022-11-30 조작된 고활성 omni-79 뉴클레아제 변이체

Country Status (9)

Country Link
US (1) US20250043260A1 (https=)
EP (1) EP4441210A4 (https=)
JP (1) JP2024542883A (https=)
KR (1) KR20240135608A (https=)
CN (1) CN118647715A (https=)
AU (1) AU2022401869A1 (https=)
CA (1) CA3239753A1 (https=)
IL (1) IL313245A (https=)
WO (1) WO2023102407A2 (https=)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US12091688B2 (en) 2021-02-08 2024-09-17 Emendobio Inc. OMNI-103 CRISPR nuclease
KR20230142740A (ko) 2021-02-08 2023-10-11 에멘도바이오 인코포레이티드 Omni-103 crispr 뉴클레아제

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4400597A3 (en) * 2015-10-09 2024-10-16 Monsanto Technology LLC Novel rna-guided nucleases and uses thereof
AU2018240515B2 (en) * 2017-03-24 2024-07-25 CureVac SE Nucleic acids encoding CRISPR-associated proteins and uses thereof
WO2020223514A2 (en) * 2019-04-30 2020-11-05 Emendobio Inc. Novel omni-50 crispr nuclease
WO2022119881A1 (en) * 2020-12-01 2022-06-09 Emendobio Inc. Differential knockout of a heterozygous allele of lrrk2

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Publication number Publication date
EP4441210A2 (en) 2024-10-09
JP2024542883A (ja) 2024-11-18
WO2023102407A9 (en) 2024-08-29
CA3239753A1 (en) 2023-06-08
IL313245A (en) 2024-08-01
EP4441210A4 (en) 2025-09-24
WO2023102407A3 (en) 2023-08-03
US20250043260A1 (en) 2025-02-06
CN118647715A (zh) 2024-09-13
WO2023102407A2 (en) 2023-06-08
AU2022401869A1 (en) 2024-07-11

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