KR20240132357A - 친화도 크로마토그래피를 사용하여 불순물로부터 이종이량체 단백질의 분해를 개선하는 방법 - Google Patents

친화도 크로마토그래피를 사용하여 불순물로부터 이종이량체 단백질의 분해를 개선하는 방법 Download PDF

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Publication number
KR20240132357A
KR20240132357A KR1020247026326A KR20247026326A KR20240132357A KR 20240132357 A KR20240132357 A KR 20240132357A KR 1020247026326 A KR1020247026326 A KR 1020247026326A KR 20247026326 A KR20247026326 A KR 20247026326A KR 20240132357 A KR20240132357 A KR 20240132357A
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KR
South Korea
Prior art keywords
cycles
series
protein
binding
impurity
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
KR1020247026326A
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English (en)
Korean (ko)
Inventor
신디 첸
앤드류 바마스
마크 치보로스키
크리스토퍼 세빈스키
신 딘
Original Assignee
리제너론 파아마슈티컬스, 인크.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Application filed by 리제너론 파아마슈티컬스, 인크. filed Critical 리제너론 파아마슈티컬스, 인크.
Publication of KR20240132357A publication Critical patent/KR20240132357A/ko
Pending legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
    • B01D15/3804—Affinity chromatography
    • B01D15/3809—Affinity chromatography of the antigen-antibody type, e.g. protein A, G or L chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Peptides Or Proteins (AREA)
KR1020247026326A 2022-01-12 2023-01-11 친화도 크로마토그래피를 사용하여 불순물로부터 이종이량체 단백질의 분해를 개선하는 방법 Pending KR20240132357A (ko)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US202263298745P 2022-01-12 2022-01-12
US63/298,745 2022-01-12
US202263430477P 2022-12-06 2022-12-06
US63/430,477 2022-12-06
PCT/US2023/010594 WO2023137062A1 (en) 2022-01-12 2023-01-11 Methods for improving resolution of heterodimeric proteins from impurities using affinity chromatography

Publications (1)

Publication Number Publication Date
KR20240132357A true KR20240132357A (ko) 2024-09-03

Family

ID=85382626

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020247026326A Pending KR20240132357A (ko) 2022-01-12 2023-01-11 친화도 크로마토그래피를 사용하여 불순물로부터 이종이량체 단백질의 분해를 개선하는 방법

Country Status (10)

Country Link
US (1) US20230242579A1 (https=)
EP (1) EP4463463A1 (https=)
JP (1) JP2025502157A (https=)
KR (1) KR20240132357A (https=)
AU (1) AU2023207604A1 (https=)
CA (1) CA3242938A1 (https=)
IL (1) IL314032A (https=)
MX (1) MX2024008637A (https=)
TW (1) TW202337900A (https=)
WO (1) WO2023137062A1 (https=)

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MY192182A (en) 2009-06-26 2022-08-04 Regeneron Pharma Readily isolated bispecific antibodies with native immunoglobulin format
US20150093800A1 (en) * 2013-09-05 2015-04-02 Genentech, Inc. Method for chromatography reuse
GB201711481D0 (en) * 2017-07-17 2017-08-30 Ucb Biopharma Sprl Protein purification
WO2020214792A1 (en) * 2019-04-17 2020-10-22 Bristol-Myers Squibb Company Methods for regenerating chromatography resins

Also Published As

Publication number Publication date
JP2025502157A (ja) 2025-01-24
AU2023207604A1 (en) 2024-07-25
IL314032A (en) 2024-09-01
WO2023137062A1 (en) 2023-07-20
MX2024008637A (es) 2024-08-27
EP4463463A1 (en) 2024-11-20
CA3242938A1 (en) 2023-07-20
US20230242579A1 (en) 2023-08-03
TW202337900A (zh) 2023-10-01

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