JP2025502157A - アフィニティークロマトグラフィーを使用した不純物からのヘテロ二量体タンパク質の分離能を改善する方法 - Google Patents

アフィニティークロマトグラフィーを使用した不純物からのヘテロ二量体タンパク質の分離能を改善する方法 Download PDF

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Publication number
JP2025502157A
JP2025502157A JP2024541632A JP2024541632A JP2025502157A JP 2025502157 A JP2025502157 A JP 2025502157A JP 2024541632 A JP2024541632 A JP 2024541632A JP 2024541632 A JP2024541632 A JP 2024541632A JP 2025502157 A JP2025502157 A JP 2025502157A
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cycles
series
protein
chromatography
affinity matrix
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JP2024541632A
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Japanese (ja)
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JP2025502157A5 (https=
Inventor
シンディ チェン、
アンドリュー バルマッセ、
マーク チボロスキ、
クリストファー セヴィンスキ、
ショーン ディン、
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Regeneron Pharmaceuticals Inc
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Regeneron Pharmaceuticals Inc
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Application filed by Regeneron Pharmaceuticals Inc filed Critical Regeneron Pharmaceuticals Inc
Publication of JP2025502157A publication Critical patent/JP2025502157A/ja
Publication of JP2025502157A5 publication Critical patent/JP2025502157A5/ja
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
    • B01D15/3804—Affinity chromatography
    • B01D15/3809—Affinity chromatography of the antigen-antibody type, e.g. protein A, G or L chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Peptides Or Proteins (AREA)
JP2024541632A 2022-01-12 2023-01-11 アフィニティークロマトグラフィーを使用した不純物からのヘテロ二量体タンパク質の分離能を改善する方法 Pending JP2025502157A (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US202263298745P 2022-01-12 2022-01-12
US63/298,745 2022-01-12
US202263430477P 2022-12-06 2022-12-06
US63/430,477 2022-12-06
PCT/US2023/010594 WO2023137062A1 (en) 2022-01-12 2023-01-11 Methods for improving resolution of heterodimeric proteins from impurities using affinity chromatography

Publications (2)

Publication Number Publication Date
JP2025502157A true JP2025502157A (ja) 2025-01-24
JP2025502157A5 JP2025502157A5 (https=) 2026-01-20

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ID=85382626

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2024541632A Pending JP2025502157A (ja) 2022-01-12 2023-01-11 アフィニティークロマトグラフィーを使用した不純物からのヘテロ二量体タンパク質の分離能を改善する方法

Country Status (10)

Country Link
US (1) US20230242579A1 (https=)
EP (1) EP4463463A1 (https=)
JP (1) JP2025502157A (https=)
KR (1) KR20240132357A (https=)
AU (1) AU2023207604A1 (https=)
CA (1) CA3242938A1 (https=)
IL (1) IL314032A (https=)
MX (1) MX2024008637A (https=)
TW (1) TW202337900A (https=)
WO (1) WO2023137062A1 (https=)

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MY192182A (en) 2009-06-26 2022-08-04 Regeneron Pharma Readily isolated bispecific antibodies with native immunoglobulin format
US20150093800A1 (en) * 2013-09-05 2015-04-02 Genentech, Inc. Method for chromatography reuse
GB201711481D0 (en) * 2017-07-17 2017-08-30 Ucb Biopharma Sprl Protein purification
WO2020214792A1 (en) * 2019-04-17 2020-10-22 Bristol-Myers Squibb Company Methods for regenerating chromatography resins

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Publication number Publication date
AU2023207604A1 (en) 2024-07-25
IL314032A (en) 2024-09-01
WO2023137062A1 (en) 2023-07-20
MX2024008637A (es) 2024-08-27
EP4463463A1 (en) 2024-11-20
KR20240132357A (ko) 2024-09-03
CA3242938A1 (en) 2023-07-20
US20230242579A1 (en) 2023-08-03
TW202337900A (zh) 2023-10-01

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