KR20240074320A - Antioxidant composition for feed additive and feed comprising thereof - Google Patents

Abstract

The present invention relates to an antioxidant composition for adding to livestock feed containing L-glutamic acid and glycine, and to livestock feed containing the same. When added to livestock feed, the antioxidant composition for livestock feed of the present invention increases the level of glutathione in the body of livestock, exhibits excellent antioxidant and anti-stress effects, and increases growth and productivity of livestock.

Description

Antioxidant composition for feed additive and feed comprising the same {Antioxidant composition for feed additive and feed comprising thereof}

The present invention relates to an antioxidant composition for adding to livestock feed containing a glutathione precursor and to livestock feed containing the same.

Aging as well as disease infection increases oxidative stress in cells or the body and increases the production of free radicals. All cells synthesize GSH in the intracellular cytoplasm using glutathione (GSH) precursors. GSH, synthesized in the cytoplasm, protects intracellular mitochondria, cytoplasm, and intracellular nuclei from oxidative stress and is the most useful antioxidant substance used by cells. When the amount of GSH synthesis within a cell decreases, major organelles such as mitochondria and nuclei within the cell are exposed to free radical attack and are greatly damaged and unable to perform normal functions, which eventually leads to cell death. Cell death ultimately causes tissue function to decline and increases oxidative stress, which reduces an individual's immunity and makes it susceptible to disease.

Conventional methods to increase antioxidant power include administering a composition that stimulates cells to express genes that produce antioxidant enzymes, but this method uses a complex process and has the disadvantage of excessive consumption of various intracellular substances and energy. there is.

In addition, there are cases where GSH is administered directly to livestock to provide antioxidant power to the body, but this method is not only expensive, and although GSH can exhibit antioxidant power outside the cells, that is, in the digestive tract, it does not enter the cells. Rather, because it is decomposed, it has the disadvantage of not being able to acquire intracellular antioxidant power, and the GSH in the digestive tract decreases over time compared to immediately after administration, so the antioxidant power in the digestive tract also tends to decrease.

Public Patent Publication No. 10-2016-0075671

The purpose of the present invention is to provide an antioxidant composition for livestock feed that can increase growth and productivity by enhancing antioxidant power in livestock and relieving stress in livestock.

Another object of the present invention is to provide livestock feed containing the antioxidant composition for livestock feed addition.

Another object of the present invention is to provide a method for producing the antioxidant composition for adding livestock feed.

Another object of the present invention is to provide a method for producing the livestock feed.

In order to achieve the above object, the present invention,

An antioxidant composition for adding livestock feed containing L-glutamic acid and glycine is provided.

In an embodiment of the present invention, the composition contains 20 to 60% by weight of L-glutamic acid and 30 to 70% by weight of glycine.

The antioxidant composition for adding livestock feed is added in an amount of 0.01 to 10% by weight based on livestock feed.

The composition of the present invention can increase the level of glutathione in the body of livestock.

The composition of the present invention may further include antioxidants.

In an embodiment of the present invention, the antioxidant may be selected from the group consisting of vitamin C, vitamin E, beta-carotene, lycopene, bilirubin, and hypotaurine.

In an embodiment of the present invention, the composition may further include amino acids.

The present invention also provides livestock feed containing the above-described antioxidant composition for livestock feed addition.

The present invention also provides a method for producing an antioxidant composition for livestock feed, comprising mixing L-glutamic acid, glycine, and excipients in a weight ratio of 20 to 60:30 to 70:5 to 30.

The present invention also includes the steps of preparing an antioxidant composition for adding livestock feed by mixing L-glutamic acid, glycine, and excipients in a weight ratio of 20 to 60:30 to 70:5 to 30; And adding and mixing the composition in an amount of 0.01% to 10% by weight based on the total weight of livestock feed; Provides a method for manufacturing livestock feed comprising.

The antioxidant composition for livestock feed of the present invention, when added to livestock feed and fed to livestock, promotes the synthesis of glutathione in the body of livestock, increases the level of glutathione in the body, and exhibits excellent antioxidant power and anti-stress effects. do. In addition, the growth of livestock fed with livestock feed to which the antioxidant composition for livestock feed of the present invention is added is promoted and productivity increases. In addition, the antioxidant composition for adding livestock feed of the present invention can be manufactured economically and simply, providing economic benefits to farmers.

Hereinafter, the present invention will be described in detail.

The description of the structural requirements described below may be based on representative embodiments of the present invention, but the present invention is not limited to such embodiments.

The present invention provides an antioxidant composition for adding to livestock feed containing L-glutamic acid and glycine.

Glutathione (GSH) is a crystalline peptide composed of three amino acids: glutamic acid, cysteine, and glycine, and is an antioxidant in plants, animals, fungi, and some bacteria and archaea. Glutathione can prevent damage to important components of cells caused by free radicals such as free radicals, peroxides, lipid peroxides, and heavy metals. Glutathione is in the form of a tripeptide made up of three amino acids, but it is not exactly a peptide. The precursors glutamate, cysteine, and glycine synthesize glutathione through a two-step synthesis process by glutamic acid cysteine ligase (GCL) and GSH synthase within the cell.

All cells synthesize glutathione using glutathione precursors in the intracellular cytoplasm to protect the nucleus, mitochondria, and cell organelles from oxidative stress, or release glutathione synthesized using glutathione precursors within the cell to the outside of the cell to reduce oxidative stress around the cell. corresponds to In particular, glutathione synthesized within the cell can be excreted outside the cell, but glutathione outside the cell does not enter the cell, and the extracellular glutathione is decomposed in the cell membrane, so adding glutathione to feed is expensive and meaningless.

The present invention is characterized by using glutamic acid and glycine as precursors of glutathione. Glutamic acid and glycine are inexpensive and stable in the air, making them easy to handle. The composition for adding livestock feed of the present invention containing glutamic acid and glycine has the advantage of being very stable in the air, easy to add to livestock feed, and easy to digest and absorb in livestock.

In an embodiment of the present invention, the antioxidant composition for adding livestock feed contains or consists of L-glutamic acid and glycine.

When the antioxidant composition for livestock feed containing L-glutamic acid and glycine of the present invention is added to livestock feed and fed to livestock, it is digested in the livestock's body and is easily accumulated in cells, and glutathione is generated in cells, especially in the cytoplasm. It induces sufficient synthesis and increases the level of glutathione in the body. In this way, glutathione increased in the body of livestock immediately neutralizes free radicals such as reactive oxygen species generated when cells produce energy within cells, preventing cells from being damaged by these free radicals. In addition, glutathione improves the antioxidant power of cells and tissues, which can not only maintain the health of livestock but also increase growth and productivity.

In the antioxidant composition for adding livestock feed containing L-glutamic acid and glycine of the present invention, L-glutamic acid is contained at 20 to 60% by weight and glycine at 30 to 70% by weight, and preferably L-glutamic acid is contained at 30 to 50% by weight. and 40 to 60% by weight of glycine, most preferably 25 to 45% by weight of L-glutamic acid and 30 to 50% by weight of glycine. By adding it in the above-mentioned range, the absorption rate can be increased and the synthesis of glutathione can be increased in the body of livestock.

The antioxidant composition for livestock feed containing L-glutamic acid and glycine of the present invention is added in an amount of 0.01 to 10% by weight based on livestock feed. It is preferably added at 0.01 to 5% by weight, most preferably at 0.05 to 1% by weight. Even if the antioxidant composition of the present invention is added in a small proportion to livestock feed as described above, it increases the absorption rate and glutathione synthesis rate in the body of livestock, thereby preventing and treating cell damage and aging in response to oxidative stress. , can improve immunity.

In an embodiment of the present invention, the composition may further include an antioxidant. In an embodiment of the present invention, the antioxidant may be selected from the group consisting of vitamin C, vitamin E, beta-carotene, lycopene, bilirubin, hypotaurine, and mixtures thereof, and the antioxidant that can be fed to livestock is without limitation. It can be included.

In an embodiment of the present invention, the composition may further include amino acids.

The amino acids may be essential amino acids and include any combination of leucine, lysine, histidine, valine, arginine, threonine, isoleucine, phenylalanine, methionine, tryptophan, and/or any of their derivatives. In some embodiments, the amino acids may be non-essential amino acids, including any combination of alanine, asparagine, aspartate, cysteine, glutamate, glutamine, glycine, proline, serine, tyrosine, and/or any derivatives thereof. Includes. The above amino acids and/or any derivatives thereof may also include amino acids and derivatives of both non-essential and essential amino acids. The amino acids may generally be included in the composition of the present invention for nutritional support in various physiological processes in livestock, such as increasing muscle mass, providing energy, or assisting recovery.

The antioxidant may preferably be added in an amount of 0.1 to 30% by weight based on the total weight of the composition, but is not limited thereto and may be added to the composition within a range that can exhibit the effect of the present invention.

The amino acid may preferably be added in an amount of 0.1 to 30% by weight based on the total weight of the composition, but is not limited thereto and may be added to the composition within a range that can exhibit the effect of the present invention.

In addition to the L-glutamic acid and glycine, the composition for livestock feed additives further containing antioxidants and/or amino acids, when added to livestock feed and fed to livestock, improves the antioxidant power of livestock and improves the growth and growth of livestock. Productivity can be increased.

In an embodiment of the present invention, the composition may further include excipients in consideration of the stability of the formulation, binding to feed, etc. The excipients include processed grain products such as wheat flour, corn powder, and legume powder; Starches such as grain starch and starch; Calcium phosphate, calcium carbonate, calcium stearate; Sweeteners such as glucose and sugar; It may include yeast, etc., but is not limited thereto. In an embodiment of the present invention, it is preferable to use wheat flour or calcium carbonate as the excipient.

The excipient may be preferably added at 0.1 to 30% by weight, preferably 1 to 20% by weight, most preferably 5 to 30% by weight, especially 10 to 30% by weight, based on the total weight of the composition. .

The antioxidant composition for feed addition of the present invention can be applied to feed eaten by all livestock such as cows, horses, sheep, pigs, chickens, ducks, and dairy cows.

The present invention also provides livestock feed comprising the above-described composition. By adding the composition of the present invention to livestock feed and feeding it to livestock, it increases antioxidant power and immunity in livestock, relieves stress, maintains health homeostasis in livestock compared to conventional feed, and has the effect of promoting growth and productivity. there is.

The present invention also provides an antioxidant for adding livestock feed, comprising the step of mixing L-glutamic acid, glycine, and excipients in a weight ratio of 20 to 60:30 to 70:5 to 30 for L-glutamic acid:glycine:excipients. A method for producing the composition is provided. Specifically, an antioxidant composition is prepared by mixing L-glutamic acid, glycine, and excipients in a weight ratio of 20 to 60:30 to 70:5 to 30 for L-glutamic acid:glycine:excipients. In an embodiment of the present invention, the mixing ratio of L-glutamic acid, glycine, and excipients is preferably a weight ratio of 30 to 50:40 to 60:10 to 30, most preferably 25 to 25, relative to L-glutamic acid:glycine:excipient. It can be mixed at a weight ratio of 45:30 to 50:10 to 30.

The present invention also includes preparing an antioxidant composition for adding livestock feed by mixing L-glutamic acid, glycine, and excipients in a weight ratio of 20 to 60:30 to 70:10 to 20 for L-glutamic acid:glycine:excipients; And adding and mixing the composition in an amount of 0.01% to 10% by weight based on the total weight of livestock feed; Provides a method for manufacturing livestock feed comprising.

In an embodiment of the invention, the method comprises L-glutamic acid, glycine and excipients, preferably in a weight ratio of 30 to 50:40 to 60:5 to 30 for L-glutamic acid:glycine:excipient, most preferably After preparing an antioxidant composition for adding livestock feed by mixing at a weight ratio of 25 to 45:30 to 50:10 to 30, adding the prepared antioxidant composition to the livestock feed in an amount of 0.01 to 10% by weight. can do. Preferably, the composition can be mixed at 0.01 to 5% by weight, most preferably 0.01 to 1% by weight, based on livestock feed.

The method can be manufactured economically and easily using low-cost raw materials, and mass production is possible, which is beneficial to farmers.

Hereinafter, the configuration and operation of the present invention will be described in more detail through preferred embodiments of the present invention. However, this is presented as a preferred example of the present invention and should not be construed as limiting the present invention in any way.

Example

Preparation Example 1. Preparation of antioxidant composition for livestock feed addition

An antioxidant composition was prepared by mixing 30% to 40% by weight of L-glutamic acid, 40% to 50% by weight of glycine, and 10% to 30% by weight of excipients.

Example 1. Preparation of livestock feed containing the antioxidant composition of the present invention

As livestock feed, corn-soybean meal feed mixed according to the requirements of NRC (2012) was used. Livestock feed was prepared by mixing the antioxidant composition prepared in Preparation Example 1 into the livestock feed using a blender to make up 0.1% by weight of the total feed.

Example 2. Preparation of livestock feed containing the antioxidant composition of the present invention

Livestock feed was prepared by mixing the antioxidant composition prepared in Preparation Example 1 with the livestock feed of Example 1 using a blender to make up 0.2% by weight of the total feed.

Example 3. Preparation of livestock feed containing the antioxidant composition of the present invention

Livestock feed was prepared by mixing the antioxidant composition prepared in Preparation Example 1 into the livestock feed of Example 1 using a blender to make up 0.3% by weight of the total feed.

Test Example 1. Specification test

A feeding test was conducted to determine the effect of adding the antioxidant composition of the present invention to farrowing sows' feed on the blood characteristics of farrowing sows and the productivity of lactating piglets.

A total of 20 sows (Landrace The test design is 1) control group, regular feed without adding the antioxidant composition of the present invention; 2) Example 1, feed containing 0.1% by weight of the antioxidant composition of the present invention; 3) Example 2, feed with 0.2% by weight of the antioxidant composition of the present invention; 4) Example 3, a test was conducted on feed with 0.3% by weight of the antioxidant composition of the present invention, with 5 heads per 4 treatments.

The specification test was conducted at the Dankook University test farm located in Gongju-si. The test feed was an ad libitum vegetarian diet, and the water was adjusted to allow ad libitum consumption using an automatic waterer.

All data were processed using Duncan's multiple range test (Duncan, 1955) using the General Linear Model procedure of SAS (2013) to test the significance between means.

Test Example 1-1. Blood characteristics of farrowing pigs

For blood collection, 2 mL of blood was collected and analyzed from the jugular vein using a K3EDTA Vacuum tube (Becton Dicknson Vacutainer Systems, Franklin Lakes, NJ) on the 3rd, 14th, and 21st days after delivery, respectively. GSH, GSH/GSSG Ratio, and Cortisol were examined using an automatic blood analyzer (ADVID 120, Bayer, USA).

The effect of adding the antioxidant composition of the present invention to farrowing sows' feed on the blood characteristics of farrowing sows is shown in Table 1. In terms of GSH and GSH/GSSG Ratio values on the 14th day of breastfeeding, Example 3 was significantly higher than Example 1 and Example 2 (P < 0.05), and Example 1 and Example 2 were significantly higher than the control. (P < 0.05), and the GSH level of Example 3 at weaning (21 days) was significantly higher than that of Examples 1 and 2 (P < 0.05). Example 2 was significantly higher than the control (P < 0.05), and the GSH/GSSG Ratio value of Examples 2 and 3 was significantly higher than that of Example 1 (P < 0.05). Example 1 was significantly higher than the control group (P < 0.05), and the cortisol level of the control group was significantly higher than that of Examples 1 and 2 (P < 0.05). Examples 1 and Example 2 was significantly higher than Example 3 (P < 0.05).

Test Items Control Example 1 Example 2 Example 3 SEM ² 3rd day of pay GSH 10.67 11.94 11.87 11.19 1.29 GSH/GSSG Ratio 8.36 8.75 8.79 9.03 1.54 Cortisol 1.32 1.30 1.13 1.20 0.22 14th day of salary GSH 10.78 ^{b 12.21ab} 12.60 ^{ab 13.63a} 0.73 GSH/GSSG Ratio 9.58 ^b 10.77 ^ab 11.09 ^{ab 12.00a} 0.56 Cortisol 1.64 1.33 1.55 1.32 0.23 End (Day 21) GSH 11.05 ^b 12.39 ^ab 12.79 ^{ab 14.22a} 0.65 GSH/GSSG Ratio ^11.16b 12.37 ^{ab 12.88a 13.06a} 0.53 Cortisol ^{2.24a 1.99ab 1.93ab} 1.75 ^b 0.12 ² standard error
^ab When different superscripts exist in the same row, they have different meanings (P<0.05).

Test Example 1-2. Piglet productivity

The weight gain of nursing piglets was calculated by measuring body weight at birth and at weaning (21 days), and the piglet mortality rate was calculated as a percentage of the number of nursing heads at weaning (21 days) from the starting number of piglets.

The effect of adding the antioxidant composition of the present invention to farrowing pig feed on the productivity of lactating piglets is shown in Table 2.

At weaning (21 days), the body weight and daily weight gain of suckling piglets in Example 3 were significantly higher than those in Examples 1 and 2 (P < 0.05), and Examples 1 and 2 were significantly higher than those in the control group. It was significantly higher than that (P < 0.05).

Test Items

INO: Initial number of live pigs

FNO: Number of pigs left alive until the end

SUR2(Survival rate during lactation): Survival rate during lactation (%)

Body weight: average weight per head (kg/head)

Birth weight: Average weight per head at birth (kg/head)

Weaning: Average weight per pup at weaning (21 days) (kg/pup)

Average daily gain: Average daily weight gain per head (g)

Test Items Control Example 1 Example 2 Example 3 SEM ² INO 10.0 10.2 10.4 10.4 0.1 FNO 9.6 9.8 10.0 10.0 0.3 SUR2 ³ , % 96.00 96.00 96.00 96.18 3.00 Body weight, kg Birth weight 1.50 1.49 1.42 1.46 0.05 Weaning 6.68 ^{b 6.86ab 6.99ab 7.15a} 0.12 Average daily gain, g Overall ^247b 255 ^ab 265 ^{ab 271a} 6 ² standard error
^ab When different superscripts exist in the same row, they have different meanings (P<0.05).

As a result of the test, it was confirmed that the feed containing the antioxidant composition of the present invention significantly increased the concentration of glutathione (GSH) in the body of livestock. As the content of the antioxidant composition increased, the concentration of GSH tended to increase, and the concentration of GSH increased significantly at the end of feeding compared to the beginning of feeding. In addition, the GSH/GSSC ratio also significantly increased compared to the control in Examples 1 to 3, and the cortisol hormone was also decreased compared to the control, indicating a decrease in oxidative stress.

In addition, piglets born from mother pigs that consumed feed to which the antioxidant composition of the present invention was added had significantly higher body weight and feed requirement compared to the control group, confirming that they were growing quickly and being healthy.

The above description of the present invention is for illustrative purposes, and those skilled in the art will understand that the present invention can be easily modified into other specific forms without changing the technical idea or essential features of the present invention. will be.

Therefore, the embodiments described above should be understood in all respects as illustrative and not restrictive. For example, each component described as single may be implemented in a distributed manner, and similarly, components described as distributed may also be implemented in a combined form.

The scope of the present invention is indicated by the claims described later, and all changes or modified forms derived from the concept of meaning and scope of the claims should be construed as being included in the scope of the present invention.

Claims (10)

Antioxidant composition for livestock feed containing L-glutamic acid and glycine. The antioxidant composition for adding livestock feed according to claim 1, wherein the composition increases the level of glutathione in the body of livestock. The antioxidant composition for adding livestock feed according to claim 1, wherein the composition contains 20 to 60% by weight of L-glutamic acid and 30 to 70% by weight of glycine. The antioxidant composition for adding livestock feed according to claim 1, wherein the composition is added in an amount of 0.01 to 10% by weight based on the livestock feed. The antioxidant composition for adding livestock feed according to claim 1, wherein the composition further contains an antioxidant. The antioxidant composition for adding livestock feed according to claim 5, wherein the antioxidant is at least one selected from the group consisting of vitamin C, vitamin E, beta-carotene, lycopene, bilirubin, and hypotaurine. The antioxidant composition for adding livestock feed according to claim 1, wherein the composition further contains amino acids. Livestock feed comprising the composition of any one of claims 1 to 7. A method for producing an antioxidant composition for adding livestock feed, comprising the step of mixing L-glutamic acid, glycine, and excipients in a weight ratio of 20 to 60:30 to 70:5 to 30. Preparing an antioxidant composition for adding livestock feed by mixing L-glutamic acid, glycine, and excipients at a weight ratio of 20 to 60:30 to 70:5 to 30; and
Adding and mixing the composition in an amount of 0.01% to 10% by weight based on the total weight of livestock feed; containing
Method for manufacturing livestock feed.