KR20240074085A - Cosmetic composition containing brewer's yeast exosomes for alleviating hair loss symptoms and improving scalp - Google Patents

Abstract

The present invention relates to a cosmetic composition for alleviating hair loss symptoms and improving the scalp containing brewer's yeast exosomes, and more specifically, brewer's yeast exosomes prepared from brewer's yeast (Saccharomyces cerevisiae) using an ultra-high pressure nano-homogenizer. It is characterized by containing brewer's yeast exosomes as an active ingredient.
The composition of the present invention promotes the proliferation of dermal papilla cells constituting hair follicles or increases the elasticity of hair, so it is excellent in alleviating hair loss symptoms and has a significant effect in improving scalp keratin, improving scalp sebum, and improving scalp moisturization. Accordingly, the composition of the present invention can be applied to various hair or scalp products such as hair tonic, hair shampoo, and hair conditioner.

Description

Cosmetic composition containing brewer's yeast exosomes for alleviating hair loss symptoms and improving scalp {Cosmetic composition containing brewer's yeast exosomes for alleviating hair loss symptoms and improving scalp}

The present invention relates to a cosmetic composition for alleviating hair loss symptoms and improving the scalp containing brewer's yeast exosomes, and more specifically, brewer's yeast exosomes prepared from brewer's yeast (Saccharomyces cerevisiae) using an ultra-high pressure nano-homogenizer. It is characterized by containing brewer's yeast exosomes as an active ingredient.

The composition of the present invention promotes the proliferation of dermal papilla cells constituting hair follicles or increases the elasticity of hair, so it is excellent in alleviating hair loss symptoms and has a significant effect in improving scalp keratin, improving scalp sebum, and improving scalp moisturization. Accordingly, the composition of the present invention can be applied to various hair or scalp products such as hair tonic, hair shampoo, and hair conditioner.

Hair acts as a buffer against external shocks, protects the human body from external stimuli such as sunlight, temperature changes, and friction, and absorbs heavy metals such as arsenic, mercury, and zinc that are unnecessary for the human body and excretes them out of the body. Nowadays, in addition to these basic functions, the cosmetic aspect of creating a new image and creating one's own individuality and confidence is becoming more important.

However, recently hair loss, which mainly occurred in middle-aged men, is also occurring frequently in early to middle-aged people. Causes of hair loss include hair roots, sebaceous glands, etc. being affected by male hormones, decreased blood flow to the hair follicle, excessive sebum secretion, production of peroxide, scalp abnormalities due to bacterial growth, and genetic factors.

Hair loss refers to the absence of hair in areas where hair should normally exist, and generally refers to the loss of hair (thick, black hair) on the scalp. Unlike vellus hair, which has no color and is thin, virgin hair can cause cosmetic problems if it falls out. Koreans, who have lower hair density than Westerners, have about 50,000 to 70,000 hairs, and it is normal for about 50 to 70 hairs to fall out per day. However, if more than 100 hairs fall out immediately after sleeping or when washing your hair, hair loss is progressing.

This type of hair loss usually starts at the parietal region and gradually progresses to the entire head. In men, receding hair lines on both sides of the head and hair loss predominates in the parietal region, and in women, the border of hair on the facial scalp is generally well defined. It is extremely rare for a preserved, complete bald spot to form.

As mentioned above, the number of hair loss patients is increasing day by day due to exposure to the rapidly changing surrounding environment. Therefore, not only medicines to prevent hair loss and promote hair growth, but also detergents such as shampoos and soaps, cosmetics, and health foods are being actively developed. There is a situation.

In cosmetics, many raw materials are developed using animal and plant materials. In particular, most of the scalp hair loss products developed domestically are manufactured using herbal medicines such as ginseng and licorice, or by mixing plants effective in preventing hair loss such as Houttuynia cordata, perilla leaves, and green tea, but they have not yet achieved satisfactory results.

Additionally, substances such as lactic acid bacteria or yeast may be used. In particular, brewer's yeast is a by-product produced during the beer brewing process and is known to be rich in various ingredients necessary for scalp and hair health. Thanks to this, it is said that if you consume brewer's yeast regularly, your hair will become thicker and stronger, preventing it from falling out easily.

Patent Document 1 discloses a shampoo composition containing brewer's yeast extract or Lactobacillus/rice fermentation product, and does not specifically limit the extraction method or manufacturing method from brewer's yeast. Patent Document 2 discloses a composition containing brewer's yeast powder obtained by fermenting malt, where the brewer's yeast powder is commonly used on the market and contains vitamin B and biotin in amounts above predetermined standards. Patent document 3 also relates to a hair cosmetic composition using brewer's yeast extract to prevent hair loss, but the content is very small, only 0.025% by weight. In addition, Patent Document 4 also discloses an anti-hair loss cosmetic composition prepared by mixing brewer's yeast with coffee beans, ginseng root, ginger, etc., heating the mixture, and then filtering the mixture. All of the above patent documents only use brewer's yeast extract extracted by conventional methods or commercially available brewer's yeast powder.

In general, the easiest way for the active ingredients of cosmetics to be absorbed into the skin is absorption through pores, and in this case, the size of the ingredient is very important. Very easy absorption can be achieved by using exosomes (50-150 nm), which are thousands of times smaller than the diameter of pores (20,000-50,000 nm). Exosome is a compound word of EXO and Some, which is an abbreviation of Extra + Cellular + Vesicle, meaning a spherical particle that comes out of the cell.

However, to date, the development of various exosomes for application to the cosmetics industry is still minimal, and the separation and purification process for obtaining exosomes originating from cells is very complicated and has a low yield.

In order to solve the above problem, the present inventor proposed using an ultra-high pressure nanohomogenizer as an innovative manufacturing method different from the existing method (Patent Application No. 10-2022-0102279), but still uses brewer's yeast exosomes to prevent hair loss. and has never been used as a cosmetic for scalp care.

Domestic Public Patent No. 10-2020-0091611 (publication date 2020.07.31.) Domestic Public Patent No. 10-2019-0056489 (publication date 2019.05.27.) Domestic Public Patent No. 10-2022-0126354 (publication date 2022.09.16.) Domestic Published Patent No. 10-2022-0058331 (publication date 2022.05.09.)

The purpose of the present invention is to provide a cosmetic composition for alleviating hair loss symptoms and improving the scalp using brewer's yeast exosomes, a natural substance.

The present invention provides a cosmetic composition for alleviating hair loss symptoms and improving the scalp containing brewer's yeast exosomes as an active ingredient.

The brewer's yeast exosomes include the following steps: 1) cultivating brewer's yeast at room temperature to 43°C for 15 to 60 minutes; 2) pulverizing the culture medium at 30,000 to 40,000 psi using a high pressure homogenizer; 3) centrifuging the pulverized liquid; and 4) ultrafiltration of the supernatant after centrifugation.

The composition is characterized in that the formulation is one selected from hair shampoo, hair pack, and hair tonic consisting of gel, cream, and liquid.

The cosmetic composition is characterized in that it contains 0.1 to 10.0% by weight of brewer's yeast exosomes based on the total weight of the cosmetic composition.

The composition is characterized by improving the scalp by reducing the scalp keratin index and the amount of scalp sebum and increasing the amount of scalp moisture.

According to the present invention, brewer's yeast exosomes using an ultra-high pressure nano-homogenizer were applied to cosmetics and a human application experiment was performed. As a result, hair loss symptoms were alleviated or improved, such as an increase in the number of hairs.

In addition, according to the present invention, the scalp keratin index was reduced, showing an improvement effect of 31.93%, and the amount of scalp sebum was also reduced, showing an improvement effect of 53.08%. And the scalp moisture content increased, showing an effect of increasing by 24.60%.

Figure 1 shows microscopic pictures of brewer's yeast exosomes according to the present invention confirmed with Olympus CX3 according to pressure size.
Figure 2 is a graph showing the results of a test to evaluate the hair loss alleviation efficacy of a hair tonic containing brewer's yeast exosomes according to the present invention, measured according to the test period.
Figure 3 is a clinical photograph and phototrichogram showing the results of a test to evaluate the hair loss alleviation efficacy of a hair tonic containing brewer's yeast exosomes according to the present invention.
Figure 4 is a graph showing the results of a test for evaluating the scalp keratin improvement efficacy of a hair tonic containing brewer's yeast exosomes according to the present invention, measured according to the test period.
Figure 5 is an image showing the results of a test to evaluate the efficacy of hair tonic containing brewer's yeast exosomes in improving scalp keratin according to the present invention.
Figure 6 is a graph showing the results of a test to evaluate the scalp sebum improvement efficacy of the hair tonic containing brewer's yeast exosomes according to the present invention, measured according to the test period.
Figure 7 is an image showing the results of a test to evaluate the scalp sebum improvement efficacy of a hair tonic containing brewer's yeast exosomes according to the present invention.
Figure 8 is a graph showing the results of a scalp moisture content evaluation test of a hair tonic containing brewer's yeast exosomes according to the present invention measured according to the test period.

Below, preferred embodiments and experimental examples of the present invention will be described in detail, but the purpose is to provide a detailed description so that a person skilled in the art can easily practice the invention. It does not mean that the technical idea and scope of the invention are limited.

The present invention provides a cosmetic composition for alleviating hair loss symptoms and improving the scalp, comprising brewer's yeast exosomes manufactured using an ultra-high pressure nanohomogenizer as an active ingredient.

The cosmetic composition of the present invention can be formulated in various forms as cosmetics known in the art, and additional ingredients can be arbitrarily selected and appropriately mixed depending on the formulation and purpose of use. For example, it may contain conventional ingredients such as thickeners, surfactants, humectants, abrasives, sweeteners, pH adjusters, preservatives, binders, wetting agents, foaming agents, stabilizers, solubilizers, vitamins, pigments and flavorings.

In addition, the present invention provides a hair or scalp product containing the composition for alleviating hair loss symptoms and improving the scalp described above.

The hair or scalp product of the present invention can be formulated in any form that can be applied to the skin, but is preferably formulated as an external preparation. For example, it can be manufactured in a formulation that can be applied to the skin, such as liquid, cream, paste, or solid, and can be used as a shampoo, tonic, hair conditioner, hair lotion, gel, pack, etc. to promote hair growth by adding common additives. It can be manufactured into compositions such as cream, essence, powder, spray, oil, soap, liquid detergent, and liquid hair growth type, and these formulations can be used in various aerosol-type forms, but are not limited to this.

When the formulation of the present invention is in liquid form, a solvent, solubilizing agent, or emulsifying agent is used as a carrier component, such as water, ethanol, glycerin, ethyl carbonate, ethyl acetate, propylene glycol, butylene glycol, 1,2- There are hexanediol, glycerol aliphatic ester, and sorbitan fatty acid ester.

When the formulation of the present invention is in the form of paste, cream, or gel, vegetable oil, wax, starch, cellulose, silicone, bentonite, silica, zinc oxide, etc. can be used as the carrier component, and if the formulation is powder, it can be used as the carrier component. Powders such as lactose, silica, aluminum hydroxide, and calcium silicate can be used.

When the formulation of the present invention is a suspension, the carrier ingredients include water, liquid diluents such as ethanol and propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester, and polyoxyethylene sorbitan ester, Microcrystalline cellulose, aluminum metahydroxide, bentonite, agar, etc. can be used.

In one embodiment, the composition of the present invention contains an effective amount of brewer's yeast exosomes, and thus may have the effect of promoting the proliferation of dermal papilla cells constituting hair follicles or increasing the elasticity of hair.

The effective amount refers to an amount sufficient to prevent, improve, and treat hair loss. This may change appropriately depending on the degree of disease symptoms, the patient's age, weight, health status, gender, administration route, and treatment period.

Accordingly, the beer yeast used in the present invention is a yeast of the genus Saccharomyces, a by-product of beer fermentation, and Saccharomyces cerevisiae may be selected, but is not limited thereto. In the process of making beer, the yeast is separated and dried.

Yeast of the genus Saccharomyces used in the present invention include Saccharomyces ceribisia, Saccharomyces uvarum, Saccharomyces ellipsoideus, Saccharomyces Karlsbergensis, Saccharomyces sake, Saccharomyces Any one or a mixture of two or more species selected from the group consisting of Coreanus, Saccharomyces lipolytica, Saccharomyces boulardii and Saccharomyces pastorianus can be used.

The brewer's yeast (Saccharomyces yeast) contains protein, vitamin B complex, and minerals such as chromium and selenium. In particular, the vitamin B complex rich in brewer's yeast helps maintain healthy skin. In particular, selenium has an antioxidant ability that is 1,970 times stronger than that of vitamin E, so it performs an excellent function in removing free radicals that cause hair loss.

Here, vitamin B complex helps cells replicate, and chromium helps skin cells obtain energy from fat, carbohydrates, and proteins. The amino acid structure of brewer's yeast is almost similar to that of hair, so its protein can be absorbed into the hair at a high rate. In particular, it is very effective in preventing hair loss as it contains biotin, a B vitamin essential for protein metabolism for hair production, and cystine and methionine, which make up hair protein. This brewer's yeast is a natural substance and has the characteristic of having no practical side effects.

In the present invention, exosomes were isolated from the beer yeast cells discarded after beer fermentation. Exosomes are so small that they are difficult to weigh, so their separation and purification are time-consuming, cumbersome, and expensive.

Accordingly, the present invention used an ultra-high pressure nano homogenizer to quickly and economically obtain a high yield and manufactured exosomes by combining centrifugation and ultrafiltration.

Specifically, the method for producing exosomes according to the present invention includes culturing brewer's yeast; Grinding the culture medium at 10,000 to 40,000 psi using a high pressure homogenizer; and centrifuging the pulverized liquid; and ultrafiltering the supernatant after centrifugation. At this time, the crushing step is most preferably performed at a pressure of 30,000 to 40,000 psi. In addition, the grinding step is repeated three or more times.

This was manufactured in the same manner as the manufacturing method disclosed in Patent Application No. 10-2022-0102279 previously filed by the present inventor.

Hereinafter, the present invention will be described in detail through examples.

Example 1. Exosome according to the present invention

Exosomes were isolated from beer fermented with Saccharomyces cerevisiae (Leibar company yeast). Specifically, the active ingredient was extracted at high concentration after culturing by shaking in synthetic complete medium at 43°C for 45 minutes.

The extracted culture solution was pulverized three or more times at 10,000 psi using an ultra-high pressure homogenizer (also called a nanogenizer). The experimental results for this were observed under a microscope (OLYMPUS, CX-33) to observe the particle state of the beer yeast (see Figure 1). After grinding, it was first centrifuged at 10,000 rpm for 30 minutes (Hanil Science Industrial, Continent 512R Plus), and then the supernatant was ultrafiltered to collect brewer's yeast exosomes. The brewer's yeast exosomes have a particle size of 100 to 150 nm in diameter.

Example 2. Exosomes according to the present invention

The same procedure as Example 1 was performed, except that the pressure of the ultra-high pressure nanohomogenizer in Example 1 was set to 15,000 psi three times.

Example 3. Exosomes according to the present invention

The same procedure as Example 1 was performed, except that the pressure of the ultra-high pressure nanohomogenizer in Example 1 was set to 20,000 psi three times.

Example 4. Exosomes according to the present invention

It was performed in the same manner as Example 1, except that the pressure of the ultra-high pressure nanohomogenizer in Example 1 was treated three times at 25,000 psi.

Example 5. Exosomes according to the present invention

The same procedure as Example 1 was performed, except that the pressure of the ultra-high pressure nanohomogenizer in Example 1 was set to 30,000 psi three times.

Example 6. Exosomes according to the present invention

The same procedure as Example 1 was performed, except that the pressure of the ultra-high pressure nanohomogenizer in Example 1 was set to 35,000 psi three times.

Looking at the micrograph in Figure 1, it can be seen that the cell walls of beer yeast are destroyed little by little at 15,000 psi, and that from 30,000 psi and above, many of the cell walls are destroyed compared to other pressures. In particular, it was confirmed that when treated three times at 35,000 psi, all beer yeast cells except one beer yeast were destroyed. This pressure condition shows the most desirable particle state.

That is, the shape of the brewer's yeast exosomes collected according to Example 6 (35,000 psi) is a transparent liquid, the pH is 6.60 ± 0.2, the average particle size is 101.5 nm, and the obtained concentration is 420,000,000,000 particles/ml, which is Example 2 The concentration of exosomes collected at (15,000 psi) is 24 times higher than the 17,400,000,000 parts/ml. The specific experimental results for this are in Table 1. The brewer's yeast exosomes of Example 6 are most preferred.

division High pressure range (psi) Average particle size (nm) Exosome concentration (particles/ml) Example 1 10,000 147.8 18,100,000,000 Example 2 15,000 119.8 17,400,000,000 Example 3 20,000 102.6 112,000,000,000 Example 4 25,000 107.0 226,000,000,000 Example 5 30,000 107.2 334,000,000,000 Example 6 35,000 101.5 420,000,000,000

The present invention prepared a cosmetic composition for hair or scalp containing brewer's yeast exosomes prepared by the above method as an active ingredient. The composition is characterized in that the formulation is one selected from hair shampoo, hair pack, and hair tonic consisting of gel, cream, and liquid. Among these, hair tonic is the most desirable.

In addition, the cosmetic composition preferably contains 0.1 to 10.0% by weight, preferably 1.0 to 5.0% by weight, of brewer's yeast exosomes based on the total weight.

Manufacturing example. Hair tonic containing brewer's yeast exosomes according to the present invention

A liquid hair tonic was prepared using the brewer's yeast exosomes of Example 6 in a conventional manner according to the formulation shown in Table 2 below. First, purified water, brewer's yeast extract, 1,2-hexanediol, butylene glycol, and disodium EDTA are dissolved with stirring at room temperature. Mix ethanol and menthol in a separate container and mix further. In another separate container, dissolved PEG-60 hydrogenated castor oil, fragrance, and ethylhexylglycerin are mixed into the above mixture. The hair tonic mixture prepared in this way is deaerated and stored in a container.

Formulation Ingredients content(%) ethanol 47.80 Purified water 45.00 Brewer's yeast exosomes 5.00 PEG-60 Hydrogenated Castor Oil 0.80 1,2-hexanediol 0.52 menthol 0.30 Spices 0.30 Butylene glycol 0.25 Ethylhexylglycerin 0.03 Sum 100

Hair tonic samples with the above composition were requested from the Korea Dermatological Research Institute and tested on human subjects on 11 test subjects with hair loss symptoms.

Experimental Example 1. Hair loss alleviation efficacy evaluation test

In order to compare hair tonics containing brewer's yeast exosomes of the present invention (Table 2) before and after (4 weeks and 8 weeks later), hair count analysis was performed using a Folliscope Phototrichogram. The measuring device is equipment that analyzes the characteristics of hair based on photos taken using a high-resolution digital macro camera. As a result, the number of hairs (N/cm2) in the test area where the sample was used increased from 108.64 to 112.0 from 4 weeks after the test compared to before the test (see Figure 2), and clinical photographs and phototrichograms (Figure 2) for 2 out of 11 test subjects 3), it was confirmed that hair loss symptoms were alleviated or improved 4 weeks after the test.

Experimental Example 2. Efficacy evaluation test for improving scalp keratin

As a result of a scalp keratin test on the hair tonic with the composition shown in Table 2, the scalp keratin index decreased after 4 weeks of testing compared to before the test. The test area was approximately 15 cm away from the eyebrows on an imaginary line connecting the eyebrows and the crown of the head, and the keratin index was evaluated using Comeofix and Visioscan. The scalp keratin index was 13.4 before the test, but after 4 weeks of the test it decreased to 9.58, showing an improvement rate of 27.71%, and after 8 weeks of the test, it decreased to 9.05, showing an improvement rate of 31.93% (see Figure 4). Image data of keratin improvement for 2 out of 11 test subjects can be seen in Figure 5, showing that keratin gradually decreases over time.

Experimental Example 3. Efficacy evaluation test for improving scalp sebum

As a result of a scalp sebum test on the hair tonic with the composition shown in Table 2, the amount of scalp sebum decreased after 4 weeks of testing compared to before the test. The test area was about 15cm away from the eyebrows on the imaginary line connecting the eyebrows and the crown of the head, and the amount of sebum was evaluated using Visioscan and Sebufix. The amount of scalp sebum (μg/cm2) was 2.80 before the test, but after 4 weeks of the test, it decreased to 1.56, showing an improvement rate of 38.78%, and after 8 weeks of the test, it decreased to 0.96, showing an improvement rate of 53.08% (Figure 6 reference). Image data showing improved scalp sebum for two of the 11 test subjects can be seen in Figure 7, showing that the amount of sebum gradually decreases over time.

Experimental Example 4. Scalp moisture content evaluation test using Hydration Pin

As a result of a scalp moisture content test on the hair tonic with the composition shown in Table 2, the scalp moisture content increased after 4 weeks of testing compared to before the test. The test site was approximately 15 cm away from the eyebrows on an imaginary line connecting the eyebrows and the crown of the head, and the moisture content was evaluated using a Hydration Pin (DermaLab). The moisture content (μS) was 35.73 before the test, but after 4 weeks of the test, it increased by 12.59% to 39.64, and after 8 weeks of the test, it increased to 43.91 by 24.60% (see Figure 8).

According to the above experimental examples, it was confirmed that the hair tonic containing brewer's yeast exosomes according to the present invention is significantly effective in alleviating hair loss symptoms, as well as improving scalp keratin, improving scalp sebum, and improving scalp moisturization.

This is because brewer's yeast exosomes, which were previously difficult to apply, were applied to cosmetic compositions. In other words, by using an ultra-high pressure nano homogenizer, both the exosomes inside the cells that have not yet been released from the cells and the exosomes that have already been released outside the cells are obtained, increasing productivity and yield, and having the effect of sterilizing all unnecessary bacteria, etc., reducing skin toxicity. Since it was possible to obtain exosomes with almost no hair loss symptoms, it was possible to manufacture a cosmetic composition that not only has the effect of alleviating hair loss symptoms but is also safe when applied to the human body.

In the above, the present invention has been described in detail focusing on the embodiments, but it is obvious to those skilled in the art that various changes and modifications are possible within the technical scope of the present invention, and it is natural that such changes and modifications fall within the scope of the appended patent claims.

Claims (5)

Cosmetic composition for relieving hair loss symptoms and improving scalp, containing brewer's yeast exosomes as an active ingredient In claim 1,
A cosmetic composition characterized in that the brewer's yeast exosomes are manufactured through the following steps.
1) Cultivating brewer's yeast at room temperature to 43°C for 15 to 60 minutes;
2) pulverizing the culture medium at 30,000 to 40,000 psi using a high pressure homogenizer;
3) centrifuging the pulverized liquid; and
4) Ultrafiltration of the supernatant after centrifugation In claim 1 or claim 2,
The composition is a cosmetic composition, characterized in that the formulation is one selected from hair shampoo, hair pack, and hair tonic consisting of gel, cream, and liquid. In claim 1 or claim 2,
A cosmetic composition containing 0.1 to 10.0% by weight of the brewer's yeast exosomes based on the total weight of the cosmetic composition. In claim 1 or claim 2,
The composition is a cosmetic composition characterized in that the scalp is improved by reducing the scalp keratin index and the amount of scalp sebum and increasing the amount of scalp moisture.