KR20240072538A - Health functional composition using Hwangchil tree liquid - Google Patents

Abstract

It relates to a composition for preventing or treating neuroinflammatory diseases containing an extract of Dendropanax Morbifera, and more specifically, to a composition for preventing or treating neuroinflammatory diseases by reducing the production of neurotoxic factors or inflammatory cytokines generated in microglia. It relates to a composition containing Hwangchil tree extract as an active ingredient that can more effectively prevent or treat diseases. The Hwangchil radish extract of the present invention not only does not show toxicity to cells, but also effectively inhibits the activity of microglial cells related to neuroinflammation. Therefore, it can be useful as a health functional food for the prevention or treatment of neuritis diseases such as Alzheimer's disease, Parkinson's disease, Huntington's disease, Lou Gehrig's disease, Creutzfeldt-Jakob disease, multiple sclerosis, etc., or for the prevention or improvement of neuroinflammatory diseases.

Description

Health functional composition using Hwangchil tree liquid}

It relates to a composition for preventing or treating neuroinflammatory diseases containing an extract of Dendropanax Morbifera, and more specifically, to a composition for preventing or treating neuroinflammatory diseases by reducing the production of neurotoxic factors or inflammatory cytokines generated in microglia. It relates to a composition containing Hwangchil tree extract as an active ingredient, which can prevent or treat diseases more effectively.

Inflammatory reactions occur when tissue (cells) are damaged or infected with external infectious agents (bacteria, mold, viruses, various types of allergens), and various inflammatory mediators and immune cells in local blood vessels and body fluids are involved, resulting in enzyme activation and inflammation mediation. It shows a series of complex physiological reactions such as substance secretion, fluid infiltration, cell movement, and tissue destruction, as well as external symptoms such as erythema, edema, fever, and pain. In normal cases, the inflammatory response removes external infectious agents and regenerates damaged tissues to restore the function of the organism, but if the inflammatory response occurs excessively or continuously due to antigens not being removed or internal substances as the cause, it actually promotes mucosal damage, resulting in In some cases, it leads to diseases such as cancer. Recently, it has been revealed that inflammatory response is one of the main mechanisms causing neurodegeneration. Microglia are the primary immune system in the Central Nervous System (CNS). As a cell that performs a function, it maintains the shape of long, thin branches and a thin cell body. When toxins introduced from outside or generated internally are present, the cells form thick and short branches and a round shape to protect the nerve cells from these toxins. It changes into an activated shape with a cell body. Unlike normal microglia, activated microglia actively engage in phagocytosis, proliferate, and release cytokines such as TNF-α, IL-1β, and IL-6, chemokines, iNOS (induciblenitricoxide synthase), and COX. Genes such as -2 (cyclooxygenase-2) are expressed to produce inflammatory mediators. Activation of microglial cells removes damaged cells and protects nerve cells from invading bacteria or viruses. However, nitric oxide produced by excessively expressed iNOS and prostaglandins produced by COX-2, Since TNFα and other substances are also toxic to nerve cells, activation of microglial cells ultimately worsens damage to nerve cells. In addition, since substances released by dying nerve cells trigger the activity of microglial cells again, neurodegeneration falls into a continuous vicious cycle. In fact, it has been reported that the activity of microglial cells is related to various neurodegenerative diseases such as Alzheimer's disease, Parkinson's disease, Huntington's disease, Lou Gehrig's disease, Creutzfelt-Jakob Disease (CJD), and multiple sclerosis. Activating substances include lipopolysaccharide (LPS), a bacterial endotoxin, interferon-γ, beta-amyloid, and ganglioside. The signal transduction system involved in the activation of microglial cells is MAPK. These include PKC, ROS, and NF-kB. The mitogen-activated protein (MAP) kinase family is a key protein as an intracellular signal transduction mediator and is activated in response to various extracellular signals such as inflammatory response, death, cell differentiation, and growth in the human body, and acts as a transcription factor. Activates and regulates the transcription of necessary genes. There are three known MAPkinase families: Erk (extracellular signal regulated kinase), JNK (c-jun N-terminal kinase), and p38 MAPkinase. Erk is closely related to cell growth and is regulated by many cell growth factors. JNK and p38 MAP kinase are closely related to most inflammatory responses, cell stress signals, and cell death signals.

The promoters of the iNOS, TNF-α, and COX-2 genes expressed in activated microglial cells have a common region where NF-kB binds, and the expression of these genes is regulated by activation of NF-kB. It is known that beta-amyloid and LPS activate NF-kB in microglial cells, and gangliosides and thrombin also activate NFkB in microglial cells. Activation of NF-κB by these activators occurs within 15 minutes and promotes the production of inflammatory cytokines. Although the relationship between microglial activation and neurodegenerative diseases has not yet been fully elucidated, the activity of microglial cells is generally It is accepted that it is involved in the onset and progression of this neurodegenerative disease. Therefore, suppressing the activity of microglial cells will be an effective treatment that can alleviate the progression of degenerative neurological diseases. Meanwhile, Dendropanax morbifera Lev. is a plant belonging to the Araliaceae family and is only found in the world in Haenam, Korea, and Haenam, Korea. It is an evergreen broad-leaved tree that grows only in parts of the southern coast such as Wando and Jeju Island. When the bark of the Hwangchil tree is wounded, a yellow resin comes out, which is called Hwangchil (黃漆). In the herbal medicine class, Hwangchil tree is known to be effective in removing fever and treating eye problems and burns. Recently, it has been reported that the leaves of Hwangchil tree are effective in anti-cancer, and also have antioxidant and nerve stabilizing effects. It is known that Hwangchil tree extract helps whiten skin by inactivating tyrosinase, which produces melanin pigment in the skin. Hwangchil tree consists of 66.7% non-volatile components, 10.8% aromatic components, 8.1% moisture, and 14.4% solid content. In particular, the aromatic components are mainly β-cubebene, γ-selinene, and δ-cardio. It is reported to be composed of cadiene.

Recently, natural compounds isolated from medicinal plants have been studied for various human treatments. Since plant products generally have lower side effects and toxicity than chemically synthesized medicines, plant-derived substances are attracting attention as biochemical active agents in the treatment of many diseases, including neuroinflammation. Accordingly, the present inventors effectively activated microglial cells. As a result of diligent efforts to select natural substances that inhibit Hwangchil tree extract, not only is it not toxic to cells, but it also causes tumor necrosis in microglial cells activated by LPS.

Significantly suppresses the increase in inflammatory cytokines such as factor-α (TNF-α; tumor necrosis factor-alpha), nitric oxide (NO), and interleukin-6 (IL-6), and increases MAPK It was confirmed that neuroinflammation caused by microglial cells was effectively reduced by reducing the phosphorylation of mitogen-activated proteinkinases and the activity of NF-κB (nuclear factor-kappaB), and the present invention was completed.

The first problem to be solved by the present invention is to provide a pharmaceutical composition for preventing or treating neuroinflammatory diseases containing an extract of Dendropanax Morbifera as an active ingredient. The second problem to be solved by the present invention is to provide a health functional food for preventing or improving neuroinflammatory diseases containing Dendropanax Morbifera extract as an active ingredient.

Dendropanax Morbifera extract is used as an active ingredient.

It includes a pharmaceutical composition for preventing or treating neuroinflammatory diseases or a health functional food composition for preventing or improving neuroinflammatory diseases. According to a preferred embodiment of the present invention, the Hwangchil tree extract is extracted from Hwangchil tree leaves. It could be.

According to another preferred embodiment of the present invention, the Hwangchil tree extract may be extracted with one or more solvents selected from the group consisting of water, alcohol, ethyl acetate, chloroform, butanol, and hexane. Another preferred embodiment of the present invention According to, the Hwangchil tree extract may be included in the total composition at a concentration of 1 to 100 ㎍/㎖. According to another preferred embodiment of the present invention, the Hwangchil tree extract may be used in microglia, which are immune cells of the nervous system. It can inhibit the activity of or exert a protective effect against damage caused by activated microglial cells to nerve cells.

According to another preferred embodiment of the present invention, the Hwangchil tree extract is selected from the group consisting of tumor necrosis factor-α (TNF-α; tumornecrosis factor-alpha) and interleukin-6 (IL-6; interleukin-6). It can inhibit the production of one or more inflammatory cytokines or nitric oxide (NO). According to another preferred embodiment of the present invention, the Hwangchil tree extract contains Erk (extracellular signal regulated kinase), JNK ( Inhibits the phosphorylation of one or more MAPKs (mitogen-activated protein kinases) selected from the group consisting of c-jun N-terminal kinase) and p38 (p38 MAP kinase), or inhibits the activity of NF-κB (nuclear factor-kappaB). can be reduced.

According to another preferred embodiment of the present invention, the neuroinflammatory disease may be any one selected from the group consisting of Alzheimer's disease, Parkinson's disease, Huntington's disease, Lou Gehrig's disease, Creutzfeldt-Jakob disease, and multiple sclerosis.

Not only is the Hwangchil tree extract not toxic to cells, but it also inhibits tumor necrosis factor-α (TNF-α; tumor necrosis factor-alpha), nitric oxide (NO), and interleukin in microglial cells activated by LPS. Significantly suppresses the increase in inflammatory cytokines such as IL-6 (interleukin-6) and reduces the phosphorylation of MAPK (mitogen-activated protein kinases) and the activity of NF-κB (nuclear factor-kappaB), thereby reducing neuroinflammation. Since it effectively inhibits the activity of microglial cells related to the disease, it has health functions for preventing or treating neuroinflammatory diseases such as Alzheimer's disease, Parkinson's disease, Huntington's disease, Lou Gehrig's disease, Creutzfeldt-Jakob disease, multiple sclerosis, etc., or for preventing or improving neuroinflammatory diseases.

Can be useful as food

Figure 1 shows the survival rate of BV2 cells, which are microglial cells, by extracts containing hexane-soluble extract (DMLH; A and D), saturated butanol-soluble extracts (DMLB; B and E), and chloroform-soluble extracts (DMLC; C and F). (A, B, and C) This is data confirming the difference and the change in nitric oxide (NO) production (D, E, and F). Figure 2 shows the monooxidation of BV2 cells, which are microglial cells, by the ethyl acetate soluble extract of Hwangchil tree of the present invention. Data confirming the change in nitrogen (NO) production (A) and cell survival rate (B). Figure 3 shows TNF-α (A) and IL-6 (B) of BV2 cells, which are microglial cells, produced by the Hwangchil tree extract of the present invention. ) This is data confirming the change in production amount.
Figure 4 shows data confirming the degree of phosphorylation of p38 (A and B), JNK (C and D), and Erk (E and F) over time when BV2 cells, which are microglial cells, were stimulated with LPS.
Figure 5 shows data confirming the inhibitory effect on phosphorylation of p38 (A and B), JNK (C and D), and Erk (E and F) in BV2 cells, which are microglial cells, by the Hwangchil tree extract of the present invention.
Figure 6 is data confirming the inhibitory effect of NF-κB activity of BV2 cells, which are microglial cells, by the Hwangchil tree extract of the present invention.

Unlike normal microglia, activated microglia increase the production of cytokines such as TNF-α, IL-1β, and IL-6 and inflammatory mediators such as nitric oxide and mitogen activated proteins. protein, MAP) and NF-κB, which are activated to worsen damage to nerve cells, causing various neuroinflammatory diseases. The present invention seeks to solve the above-mentioned problems by providing a pharmaceutical composition for preventing or treating neuroinflammatory diseases or a food composition for preventing or improving neuroinflammatory diseases containing an extract of Dendropanax Morbifera as an active ingredient. Through this, the production of inflammatory mediators occurring in activated microglial cells is significantly suppressed, and the activity of mitogen-activated protein (MAP) and NF-kB is significantly suppressed to more effectively prevent neuroinflammatory diseases. It has a preventive or therapeutic effect. Therefore, the present invention includes a pharmaceutical composition for preventing or treating neuroinflammatory diseases containing an extract of Dendropanax Morbifera as an active ingredient. In the present invention, the extract of Dendropanax Morbifera may be extracted from leaves of Dendropanax Morbifera. there is. All parts of the Hwangchil tree, including leaves, stems, roots, and seeds, can be used, but parts other than the Hwangchil tree leaves may lack the effect of suppressing nerve inflammation, so it is most desirable to use the leaves of the Hwangchil tree. The Hwangchil tree extract is used alone or in a mixed form such as water, alcohol, ethyl acetate, chloroform, butanol, or hexane.

It can be extracted with a phosphorus solvent, and more preferably, ethyl acetate can be used. It can be extracted by applying a general solvent extraction method using the above solvent, and can also be a fraction purified using column chromatography. The method for producing the extract according to the present invention can apply all plant extraction methods known to those skilled in the art, such as extraction with water, alcohol or mixed solvent, extraction with double boiling or room temperature, etc. Including, but not limited to.

In one embodiment of the present invention, hexan, butanol, chloroform, and ethyl acetate were used as solvents to extract Hwangchil tree leaf extract, and when extracted using ethyl acetate, butanol, It was confirmed that the neuroinflammatory activity of activated microglial cells was more effectively inhibited compared to other solvents such as chloroform or hexane. In one embodiment of the present invention, in order to determine the neuroinflammatory inhibitory effect of each extract, rat-derived microglia were used. After pre-treating BV2 cells, which are glial cells, with Hwangchil tree extract, the BV2 cells were stimulated with LPS to observe the efficacy of inhibiting the production of inflammatory mediators and cytotoxicity caused by LPS stimulation. LPS (well-known as an endotoxin) lipopolysaccharide) is the most well-known external factor involved in the activation of macrophages, especially tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL6), and IL-1β in macrophages such as RAW 264.7 cells and monocytes. It is known that pro-inflammatory cytokines such as (interleukin-1 beta) are secreted and increased in inflamed areas. Additionally, when LPS stimulates macrophages, iNOS (inducible

Nitric oxide (NO) is produced in the process of converting L-arginine into L-citrulline by an enzyme called nitric oxide synthase, thereby producing NO from macrophages. In mammals, NO is synthesized by three types of nitric oxidesynthase (NOS): neuronal NOS (nNOS), endothelial NOS (eNOS), and inducible NOS (iNOS). Among these

NO produced by nNOS and eNOS is produced for normal biological functions, and its concentration in tissues is maintained at a low level. However, NO produced by iNOS is excessively produced and exhibits harmful effects on the living body such as pathological vasodilatation, cytotoxicity, and tissue damage. As shown in Figures 1A and 1C, sulfur hexane soluble extract (DMLH), Cytotoxicity was observed in samples treated with more than 50㎍/㎖ of Chlorophyll chloroform-soluble extract (DMLC), and as shown in Figures 1B and 2A, ethyl acetate-soluble extract (DMLE), Butanol-soluble extract (DMLB), or LPS No cytotoxicity was observed. In addition, as shown in Figures 1D to 1F and Figure 2A, it was confirmed that when four types of Hwangchil tree leaf extracts were treated regardless of the extraction solvent, the production of nitrogen monoxide was reduced in a treatment concentration-dependent manner, and Hwangchil tree leaves extract 1

When treated at a concentration of 100 to 100 ㎍/㎖, it was confirmed that the production of nitric oxide was reduced by about 10 to 85% compared to the control group stimulated with LPS. Among them, it was confirmed that the nitrogen monoxide-reducing effect of D. dwarf ethyl acetate soluble extract (DMLE) was most noticeable. The pharmaceutical composition for preventing or treating neuroinflammatory diseases of the present invention contains the D. D. Chil. ethyl acetate soluble extract (DMLE) at a concentration of 1 to 100 μg/ml. It can be included in concentration. The solvent of the composition is not particularly limited as long as it does not interfere with the neuroinflammation inhibitory effect of the Hwangchil tree extract, but more preferably, it may be water, ethanol, propyl alcohol, DMSO, etc. If the concentration of the Hwangchil tree extract composition is less than 1 ㎍/㎖, the effect of inhibiting the production of inflammatory mediators in microglial cells may be insufficient, and the efficacy of suppressing neuroinflammation may be reduced. In addition, although a higher amount of Hwangchil tree extract than 100 ㎍/㎖ may be included, sufficient neuroinflammation inhibitory activity can be confirmed even within the above range, and if it exceeds 100 ㎍/㎖, the efficacy is improved due to an increase in concentration. There may be problems with low efficiency. The concentration of the Hwangchil tree extract composition, which exhibits excellent neuroinflammation inhibition effect without cytotoxic effect, may be more preferably 10 to 100 ㎍/㎖. In the present invention, the Hwangchil tree extract of the present invention can inhibit the activity of microglia, which are immune cells of nerve cells, and can exhibit a protective effect against damage caused by activated microglia to nerve cells. there is. The Hwangchil tree extract increases the inflammatory cytokines of tumor necrosis factor-α (TNF-α; tumor necrosis factor-alpha) or interleukin-6 (IL-6; interleukin-6) and nitric oxide (NO). can be suppressed,

One or more mitogen-activated protein kinases (MAPKs) or nuclear factor kappaB (NF-κB) selected from the group consisting of extracellular signal regulated kinase (Erk), c-jun N-terminal kinase (JNK), and p38 MAP kinase (p38) As shown in Figure 2A, when the Hwangchil tree leaf extract was pretreated at different concentrations, the concentration of nitric oxide produced in BV2 cells stimulated by LPS was measured. As a result, the Hwangchil tree leaf extract treatment It was confirmed that the production of nitric oxide decreased in a concentration-dependent manner, and when the Hwangchil tree leaf extract was treated at a concentration of 1 to 100 ㎍/㎖, the production of nitric oxide was reduced by about 10 to 85% compared to the LPS-stimulated control group. confirmed. In addition, as a result of confirming the cytotoxicity of the Hwangchil tree leaf extract under the above conditions, no cytotoxicity due to the Hwangchil tree leaf extract or LPS was observed, as shown in Figure 2B.

When the Hwangchil tree leaf extract was pretreated at different concentrations, the concentrations of TNF-α and IL-6 produced in BV2 cells stimulated by LPS were measured. As shown in Figure 3, the Hwangchil tree leaf extract treatment concentration depended. It was confirmed that the production of TNF-α and IL-6 was suppressed. When treated with Hwangchil tree leaf extract at a concentration of 10 to 100 ㎍/㎖, it was confirmed that the production of TNF-α and IL-6 was reduced by about 60 to 85% and about 30 to 85%, respectively, compared to the LPS-stimulated control group. .In addition, in one embodiment of the present invention, the inhibitory effect of MAPK activity of BV2 cells stimulated with LPS by Hwangchil tree extract was observed in the same manner as Example 3. MAPK (mitogen-activated protein kinase) is a key protein as a signal transduction mediator within cells. It is activated in response to extracellular signals such as various inflammatory reactions, death, cell differentiation, and growth in the human body, and is required to activate transcription factors.

There are three known genes that regulate the transcription of genes, including Erk (extracellular signal regulated kinase), JNK (c-jun N-terminalkinase), and p38 MAP kinase. Erk is closely related to cell growth and is regulated by many cell growth factors. JNK and p38 MAP kinases regulate most inflammatory responses, cellular stress signals, and cell

It is closely related to death signals, etc. First, to determine whether changes in MAPK (mitogen-activated protein kinases) activity occur in BV2 cells due to LPS stimulation, p38 (p38 MAP kinase), JNK (c) changes over time after LPS treatment. The degree of phosphorylation of -jun N-terminal kinase) and Erk (extracellular signal regulated kinase) was measured. As shown in Figure 4, LPS stimulation increased the phosphorylation of p38 (Figures 4A and B), JNK (Figures 4C and D), and Erk (Figures 4E and F) by 5-, 10-, and 2.5-fold, respectively, in BV2 cells. It was confirmed that However, as shown in Figure 5, when BV2 was pretreated with Hwangchil tree leaf extract and then stimulated with LPS, it was confirmed that the phosphorylation of p38, JNK, and Erk decreased compared to the LPS-stimulated control group, especially p38 and Phosphorylation of JNK is present

It was confirmed that p38 and JNK were suppressed by about 40 to 50% (Figures 5A and B) and about 80 to 90% (Figure 5C), respectively, compared to the LPS-stimulated control group when treated with Hwangchil tree leaf extract. and D) It was confirmed that phosphorylation was reduced, and in the case of Erk, when treated with Hwangchil tree leaf extract, phosphorylation was confirmed to be reduced by about 20 to 25% compared to the LPS-stimulated control group (Figure 5E and F) .In addition, in one embodiment of the present invention, the inhibitory effect of NF-κB activity in BV2 cells stimulated with LPS by Hwangchil tree extract was observed in the same manner as in Example 4. NF-κB is an important transcription factor in inflammation. , NF-κB is known to regulate the expression of proteins including iNOS, TNF-α, COX-2, and MARK signaling mechanisms in activated microglia.

As shown in Figure 6, when BV2 was treated with only LPS, NF-κB activity increased by about 4-fold or more, whereas when pretreated with Hwangchil tree leaf extract, NF-κB activity decreased by about 40 to 70% compared to the LPS-stimulated control group. It was confirmed that κB activity was decreased. Therefore, the Hwangchil tree extract of the present invention not only does not show toxicity to cells, but also significantly reduces inflammatory mediators generated in activated microglial cells, phosphorylation of MAPK and NF. It was confirmed that it has the effect of significantly reducing the activity of -κB, which means that the Hwangchil tree extract of the present invention can be seen as effectively suppressing neuroinflammation caused by microglial cells. Therefore, the Hwangchil tree extract of the present invention effectively inhibits the activity of microglial cells related to neuroinflammation, and is therefore used for the prevention or treatment of neuroinflammatory diseases such as Alzheimer's disease, Parkinson's disease, Huntington's disease, Lou Gehrig's disease, Creutzfeldt-Jakob disease, multiple sclerosis, etc. As a health functional food for preventing or improving neuroinflammatory diseases

It can be usefully used. The pharmaceutical composition for preventing and treating neuroinflammatory diseases containing the extract of Hwangchil tree according to the present invention as an active ingredient may further include other natural substances or compounds that inhibit or reduce inflammation of the nerves. The pharmaceutical composition of the present invention can be administered to mammals such as rats, mice, livestock, and humans through various routes including orally, transdermally, subcutaneously, intravenously, or intramuscularly. In addition, the pharmaceutical composition for preventing or treating neuroinflammatory diseases containing the extract of Hwangchil tree according to the present invention as an active ingredient can be formulated into various dosage forms. When formulating, it can be formulated using diluents or excipients such as commonly used fillers, extenders, binders, wetting agents, disintegrants, and surfactants. Solid preparations for oral administration include tablets, pills, powders, granules, and capsules. These solid preparations include Hwangchil tree extract and at least one or more excipients (e.g., starch, sucrose, lactose, and gelatin). These can be mixed and prepared. Additionally, lubricants other than simple excipients may also be used. Liquid preparations for oral administration include suspensions, oral solutions, emulsions, and syrups. In addition to the commonly used simple diluents such as water and liquid paraffin, various excipients such as wetting agents, sweeteners, fragrances, and preservatives are included. May be included. Formulations for parenteral administration may include sterile aqueous solutions, non-aqueous solutions, suspensions, emulsions, lyophilized formulations, and suppositories. Non-aqueous solvents and suspensions may include propylene glycol, polyethylene glycol, vegetable oil such as olive oil, and injectable ester such as ethyl oleate. Glycerol and gelatin can be used as a base for suppositories.

The dosage of the pharmaceutical composition may vary depending on the subject's age, gender, condition, absorption, inactivation rate, and excretion rate of the active ingredient in the body, and concomitant drugs.

When administering to the human body a pharmaceutical composition for the prevention or treatment of neuroinflammatory diseases containing the extract of Hwangchil tree of the present invention as an active ingredient, it can be used with confidence as there is less risk of side effects compared to other synthetic medicines since it is a natural extract. .

In addition, the present invention provides a food composition for preventing or improving neuroinflammatory diseases containing the Hwangchil tree extract as an active ingredient. The types of health functional foods containing the food composition for preventing or improving neuroinflammatory diseases containing the Hwangchil tree extract of the present invention as an active ingredient are not particularly limited, and include, for example, meat, sausages, bread, chocolate, candies, and snacks. , confectionery, pizza, ramen, other noodles, gums, dairy products including ice cream, various soups, beverages, teas, drinks, alcoholic beverages, and vitamin complexes. The health foods include foods or foods other than the Hwangchil tree extract. It is used with additives and can be used appropriately according to conventional methods. For example, the drink for preventing neuroinflammatory diseases containing the Hwangchil tree extract as an active ingredient is mixed with calcium, acanthophyllum concentrate, high fructose corn syrup, purified water, etc. in addition to the Hwangchil tree extract as an active ingredient and placed in a drink bottle. A beverage can be produced by filling and sterilizing the product and then cooling it to room temperature. In addition, the health supplement for preventing neuroinflammatory diseases containing the above Hwangchil tree extract as an active ingredient contains Hwangchil tree extract, nutritional supplements (vitamins B1, B2, B5, B6, E, acetic acid ester, nicotinic acid amide), oligosaccharides, 50% Ethanol and purified water are added and mixed to form granules and dried in a vacuum dryer. Then, pass through a 12 to 14 mesh to produce uniform granules. Extrude and mold in appropriate amounts to form tablets or powder or fill into hard capsules. It can be manufactured as a hard capsule product.

The effective dose of the Hwangchil tree extract contained in the health food can be used in accordance with the effective dose of the pharmaceutical composition, and the mixing amount of the active ingredients can be appropriately determined depending on the purpose of use, such as preventive or therapeutic treatment. In the case of long-term intake for health and hygiene purposes or health control, it may be below the above range.

Claims (16)

A pharmaceutical composition for preventing or treating neuroinflammatory diseases containing an extract of Dendropanax Morbifera as an active ingredient. The Hwangchil tree extract is a pharmaceutical composition for preventing or treating neuroinflammatory diseases, characterized in that it is extracted from Hwangchil tree leaves. A pharmaceutical composition for the prevention or treatment of neuroinflammatory diseases, wherein the Hwangchil tree extract is extracted with one or more solvents selected from the group consisting of water, alcohol, ethyl acetate, chloroform, butanol, and hexane. A pharmaceutical composition for preventing or treating neuroinflammatory diseases, wherein the Hwangchil tree extract is contained in a concentration of 10 to 100 μg/ml in the total composition. The Hwangchil tree extract increases the activity of microglia, which are immune cells of nerve cells.
A pharmaceutical composition for the prevention or treatment of neuroinflammatory diseases, characterized in that it exhibits a protective effect against damage caused to nerve cells by suppressed or activated microglial cells. The Hwangchil tree extract contains one or more inflammatory cytokines or nitric oxide selected from the group consisting of tumor necrosis factor-α (TNF-α; tumor necrosis factor-alpha) and interleukin-6 (IL-6; interleukin-6). A pharmaceutical composition for preventing or treating neuroinflammatory diseases, characterized in that it inhibits the production of (NO; nitric oxide). The Hwangchil tree extract inhibits the phosphorylation of one or more MAPKs (mitogen-activated proteinkinases) selected from the group consisting of Erk (extracellular signal regulated kinase), JNK (c-jun N-terminalkinase), and p38 (p38 MAP kinase). , A pharmaceutical composition for preventing or treating neuroinflammatory diseases, characterized in that it reduces the activity of NF-κB (nuclear factor-kappaB). A pharmaceutical composition for preventing or treating neuroinflammatory diseases, wherein the neuroinflammatory diseases are at least one selected from the group consisting of Alzheimer's disease, Parkinson's disease, Huntington's disease, Lou Gehrig's disease, Creutzfeldt-Jakob disease, and multiple sclerosis. A health functional food composition for preventing or improving neuroinflammatory diseases containing extract of Dendropanax Morbifera as an active ingredient. The Hwangchil tree extract is a health functional food composition for preventing or improving neuroinflammatory diseases, characterized in that it is extracted from Hwangchil tree leaves. A health functional food composition for preventing or improving neuroinflammatory diseases, wherein the Hwangchil tree extract is extracted with one or more solvents selected from the group consisting of water, alcohol, ethyl acetate, chloroform and hexane. A health functional food composition for preventing or improving neuroinflammatory diseases, characterized in that the Hwangchil tree extract is included in the total composition at a concentration of 1 to 100 ㎍/㎖. The Hwangchil tree extract increases the activity of microglia, which are immune cells of nerve cells.
A health functional food composition for preventing or improving neuroinflammatory diseases, characterized in that it exhibits a protective effect against damage caused to nerve cells by suppressed or activated microglial cells. The Hwangchil tree extract contains one or more inflammatory cytokines or nitric oxide selected from the group consisting of tumor necrosis factor-α (TNF-α; tumor necrosis factor-alpha) and interleukin-6 (IL-6; interleukin-6). A health functional food composition for preventing or improving neuroinflammatory diseases, characterized in that it inhibits the production of (NO; nitric oxide). The Hwangchil tree extract inhibits the phosphorylation of one or more MAPKs (mitogen-activated proteinkinases) selected from the group consisting of Erk (extracellular signal regulated kinase), JNK (c-jun N-terminalkinase), and p38 (p38 MAP kinase). , A health functional food composition for preventing or improving neuroinflammatory diseases, characterized in that it reduces the activity of NF-κB (nuclear factor-kappaB). A health functional food composition for preventing or improving neuroinflammatory diseases, wherein the neuroinflammatory diseases are at least one selected from the group consisting of Alzheimer's disease, Parkinson's disease, Huntington's disease, Lou Gehrig's disease, Creutzfeldt-Jakob disease, and multiple sclerosis.