KR20240067539A - Composition for treating of dental caries comprising Magnolia flower extract - Google Patents

Abstract

The present invention relates to an oral composition and health food for the prevention or treatment of dental caries containing a kidney extract as an active ingredient. The kidney extract has an excellent antibacterial effect against dental caries bacteria, and is effective in inhibiting the growth and growth of dental caries bacteria. It can be usefully used as an oral composition and health functional food to prevent or treat dental caries by inhibiting the activity of glucosyltransferase, an enzyme released, and inhibiting the attachment of dental caries bacteria to teeth.

Description

Composition for treating dental caries comprising Magnolia flower extract}

The present invention relates to a composition for treating dental caries containing magnolia flower extract.

The oral cavity is an important organ in the human body and is directly exposed to the outside world, so it is exposed to many microorganisms. In addition, environmental conditions are suitable for bacteria to proliferate, so they are attacked by many bacteria. Oral bacteria can cause enamel demineralization under certain circumstances and trigger dental caries. Dental caries (cavities) is one of the oral diseases caused by oral streptococci, and the incidence rate is increasing not only in Korea but also around the world due to the recent increase in the use of sweeteners.

Dental caries is a disease in which the hard tissues of teeth are destroyed due to the action of organic acids produced locally through the metabolism of carbohydrates in food by microorganisms present in the supragingival and subgingival dental plaque. Development of prevention and treatment is needed. It is a topic of interest not only in our country but also globally. Dental caries is not directly related to human life, but it directly or indirectly affects diseases of the digestive system because the chewing function of food is reduced. The morbidity rate of dental caries is particularly high in children, and most people have experienced or are experiencing pain due to dental caries, reaching 85% of 5-year-old children.

There are various causes of dental caries, but in general, lactic acid, which is produced when carbohydrates such as sugar or starch in food residue attached to the teeth are broken down by the fermentation action of bacteria residing in the oral cavity, decomposes limestone from the hard tissues of the teeth, causing dental caries. It happens. Several species of pathogenic microorganisms that cause dental caries are known, but in particular, Streptococcus mutans and Streptococcus sobrinus act as the main pathogens in the occurrence of dental caries.

Streptococcus mutans secretes an enzyme called glucosyltransferase (GTase) outside the cell or on the surface of the cell to produce glucose and fructose using sucrose in food as a substrate, and at the same time insoluble glucan, a polymer of glucose. ) is formed on the tooth surface. This glucan causes other microorganisms in the oral cavity to attach to the tooth surface, forming a dental plaque. Within this plaque, lactic acid bacteria, including Streptococcus mutans, proliferate and produce lactic acid using fructose as a carbon source, and lactic acid is captured and concentrated in adhesive glucan. This concentrated, high-concentration acid dissolves Ca2+ in calcium phosphate, a component of teeth, and dissolves enamel, the outer layer of teeth, causing dental caries.

Methods used to suppress dental caries include methods using antibiotics such as sporamycin, vancomycin, and chlorhexidine, and methods for suppressing pathogenic bacteria using organic or inorganic fluorine. etc. are used (Non-patent Document 3). However, continued use of antibiotics not only has the disadvantage of causing microorganisms to become resistant or cause side effects such as diarrhea and vomiting, but also the overuse of antibiotics inhibits the growth of bacteria residing in the oral cavity. there is. Therefore, there is an urgent need for the development of effective and safe dental caries prevention and treatment agents.

Prior art regarding dental caries treatment includes Korean Patent No. 10-1544939, “Composition for preventing or treating dental caries containing extract of Jinkyo,” which states that Jinkyo extract has an excellent antibacterial effect against dental caries bacteria and dental caries extract. It is disclosed that it inhibits the activity of glucosyltransferase, an enzyme released during phagocytic growth and bacterial growth. In addition, Korean Patent No. 10-1846512, “Pharmaceutical composition for preventing and treating oral diseases containing light-activated extract of Zantoriza as an active ingredient,” contains bacteria that cause diseases such as dental caries, periodontal disease, or actinomycosis in the oral cavity. A pharmaceutical composition for preventing and treating oral diseases is disclosed, which contains as an active ingredient a light-activated extract of Zantoriza that can kill.

Magnolia flower refers to the bud of a magnolia flower belonging to the Magnoliaceae family. The flower bud has a slightly spicy taste, so it is called 辛夷. In Korea, dried flower buds of Magnolia denudata Desrousseaux or similar plants are used, and in Japan, white magnolia (M. denudata Desr.), M. flower Pamp. (望春花), and Nippon Shini (M. denudata Desr.) kobus DC., 日本辛夷), M. salicifolia Maxim.:香辛夷, and Mudang okran (M. sprengeri Pamp.: 武當玉蘭) are used, and in China, white magnolia (M. denudata Desr) and Mangchunhwa. (M. flower Pamp., 望春花) and Mudang jade orchid (M. sprengeri Pamp., 武當玉蘭) are used. In Oriental medicine, it is considered to have warm properties and is effective in treating headaches, back pain, and rhinitis. It is also reported to treat sinusitis and have analgesic properties.

While the present inventors were conducting research to derive a natural material that was effective in preventing and improving oral diseases such as dental caries, periodontal disease, toothache, sensitive teeth, and bad breath without concerns about side effects, the Streptococcus The present invention was completed by confirming the mutans growth inhibition effect, antibacterial activity, acid production inhibition effect, and the possibility of inhibiting the attachment of dental caries bacteria to the tooth surface.

The purpose of the present invention is to provide a composition for the treatment of dental caries containing a kidney extract as an active ingredient and its use for the treatment of dental caries.

In order to achieve the above purpose,

The present invention provides a pharmaceutical composition for preventing or treating dental caries containing a kidney extract as an active ingredient.

In addition, the present invention provides an oral composition for preventing or improving dental caries containing kidney extract as an active ingredient.

In addition, the present invention provides a health functional food composition for preventing or improving dental caries containing kidney extract as an active ingredient.

In addition, the present invention provides a feed additive composition for preventing or improving dental caries containing kidney extract as an active ingredient.

In addition, the present invention includes the steps of 1) adding a solvent to the kidney to obtain an extract;

2) Concentrating the extract to obtain a concentrate; and

3) drying the concentrate.

The Xixi extract of the present invention inhibits the growth of dental caries-causing bacteria and inhibits acid production, exhibits antibacterial activity against dental caries-causing bacteria, and exhibits activity to inhibit the adhesion of dental caries bacteria to the tooth surface. It can be used to treat and prevent dental caries.

Figure 1 is a graph showing the inhibitory effect of Streptococcus mutans growth of Shinyi extract.
Figure 2 is a graph showing the effect of Shinyi extract on inhibiting Streptococcus mutans adhesion.

Hereinafter, the present invention will be described in detail.

The present invention provides a pharmaceutical composition for preventing or treating dental caries containing a kidney extract as an active ingredient.

The flower of the present invention has the scientific name of Magnolia flower , which refers to the bud of a magnolia flower belonging to the Magnoliaceae family. In Oriental medicine, it is said to have warm properties and is effective for headaches, back pain, and rhinitis, and is known to treat sinusitis and have painkilling properties.

The dental caries may be caused by one or more bacteria selected from Streptococcus mutans and Streptococcus sobrinus.

The term "dental caries" of the present invention is an infectious disease caused by bacteria living on the surface of the teeth, also called cavities, and shows symptoms of erosion and loss of the hard tissue of the teeth. Specifically, the milky-white, translucent, hard substance that covers the surface of the head of the tooth and protects the dentin of the tooth is called tooth enamel or enamel. It is damaged by acids produced when sugar, starch, etc. are broken down by oral pathogens living in the mouth. This means that the enamel of the teeth is damaged, resulting in cavities.

The main cause of dental caries is dental plaque, which is a bacterial film formed on the surface of teeth. Saliva forms a thin, sticky film on the teeth, and Streptococcus sobrinus, a type of streptococcus, forms on top of it. Oral microorganisms such as Streptococcus sobrinus and Streptococcus mutans attach to form a biofilm, creating dental plaque, which is further thickened by Fusobacterium. .

Dental caries of the present invention includes all causative bacteria that cause dental caries regardless of their type, but specifically, Streptococcus mutans, Streptococcus sanguinis, and Streptococcus sobrinus. ), Streptococcus ratti, Streptococcus criceti, Streptococcus anginosus, and one or more species selected from the group consisting of lactic acid bacteria, and more specifically, Streptococcus mutans. You can.

The Streptococcus mutans is a type of streptococcus, a gram-positive bacterium, and is also called a caries bacteria. The Streptococcus mutans grows only on the surface of teeth, and deciduous teeth are not completed until around 30 months of age. Therefore, until this period, it is difficult for mutans bacteria to proliferate like after infancy. If a child is not infected with mutans bacteria from an adult's mouth during infancy and other oral bacteria take up residence in the mouth, it becomes difficult for new mutans bacteria to enter the already balanced oral ecosystem, so the probability of developing cavities after infancy and throughout life is significantly lower. You lose. If you have already been infected with the causative bacteria through the mouth of an adult, brushing your teeth frequently and keeping your mouth clean will help prevent cavities. However, once mutans bacteria have established themselves in the oral cavity, eradication of mutans bacteria is impossible.

Additionally, these bacteria adhere well to the tooth surface, produce greater amounts of acid from sugars than other bacterial types, survive more effectively than other bacteria in acidic environments, and produce extracellular polysaccharides from sucrose. If the proportion of Streptococcus mutans in plaque is high, the risk of dental caries increases.

The Xinyi extract can inhibit the growth of Streptococcus mutans.

The Shinyi extract can inhibit the production of organic acids by Streptococcus mutans.

The Xinyi extract may exhibit antibacterial activity against Streptococcus mutans.

The Shinyi extract can inhibit the attachment of Streptococcus mutans to teeth.

The pharmaceutical composition of the present invention may contain 10 to 95% by weight of the active ingredient, Siniga extract, based on the total weight of the composition. In addition, the pharmaceutical composition of the present invention may further include one or more active ingredients that exhibit the same or similar functions in addition to the above-mentioned effective ingredients.

The term "comprising an active ingredient" in the present invention means that the extract contains a sufficient amount to achieve the efficacy or activity of the extract.

The pharmaceutical composition of the present invention may further include pharmaceutically acceptable additives.

The additives may further include suitable carriers, excipients, and diluents commonly used in the preparation of pharmaceutical compositions.

The pharmaceutical composition of the present invention can be formulated and used in the form of oral dosage forms such as powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols, external preparations, suppositories, or sterile injection solutions according to conventional methods. You can. In detail, it can be prepared using commonly used diluents or excipients such as fillers, weighting agents, binders, wetting agents, disintegrants, and surfactants.

Suitable pharmaceutically acceptable carriers and formulations are described in detail in Remington's Pharmaceutical Sciences (19th ed, 1995).

The pharmaceutical composition of the present invention can be administered orally or parenterally, and in case of parenteral administration, it can be administered by intravenous injection, subcutaneous injection, intramuscular injection, intraperitoneal injection, transdermal administration, etc.

The appropriate dosage of the pharmaceutical composition of the present invention is determined by factors such as formulation method, administration method, patient's age, weight, sex, pathological condition, food, administration time, administration route, excretion rate and response sensitivity, and degree of bone loss of the patient. It varies depending on the dose, and can be appropriately selected by a person skilled in the art. Specifically, the amount is 0.001 mg/kg to 50 mg/kg, and can be administered once or several times a day as needed.

The “administration subject” of the present invention includes, but is not particularly limited to, humans, monkeys, cows, horses, pigs, sheep, chickens, cats, dogs, mice, rabbits, etc.

The present invention provides an oral composition for preventing or improving dental caries containing a kidney extract as an active ingredient.

The kidney extract may be an extract prepared by extraction with water, alcohol, or a mixture thereof, and preferably, kidney kidney extract may be an ethanol extract or a water extract. The ethanol extract may be an extract using 30 to 99% ethanol, 40 to 90% ethanol, 50 to 80% ethanol, or 60 to 75% ethanol, but is not limited thereto.

The formulation of the oral composition containing the extract of the present invention may be selected from the group consisting of toothpaste, mouthwash, mouthwash, gum, candy, oral spray, oral ointment, oral varnish, oral rinse, and gum massage cream. However, it is not limited to this.

The toothpaste may contain one or more ingredients such as abrasives, binders, moisturizers, foaming agents, sweeteners, and flavoring agents.

The abrasives include aluminum hydroxide, anhydrous silicic acid, aluminum silicate, dicalcium phosphate, dihydroxide and anhydride, tricalcium phosphate, calcium carbonate, calcium pyrophosphate, insoluble sodium metaphosphate, tribasic magnesium phosphate, magnesium carbonate, and sulfuric acid. One or two or more of calcium, polymethyl methacrylate, and other synthetic resins can be mixed and used in an amount of about 20 to 90% of the total composition (in the case of paste toothpaste, it is 20 to 60%).

The binders include garragynin, various cellulose derivatives for thickening, gums such as xanthan gum and tragacanth gum, polyvinyl alcohol, sodium polyacrylate, polyacrylic acid/maleic acid copolymer, carboxyvinyl polymer, etc. One or two or more types of organic binders such as polymer derivatives and inorganic binders such as silica and laponite can be mixed and used in an amount of about 03 to 5% of the total composition.

Among toothpastes, paste and liquid oral compositions contain one or two or more types of moisturizers such as sorbitol, glycerin, ethylene glycol, propylene glycol, polyether glycol, and polypropylene glycol in approximately 10 to 70% of the total composition during manufacture. It can be used in mixed quantities.

The oral composition of the present invention includes menthol, Anetol, Eugenol, Limonene, Ocimene, and n-Dodecy Alcohol. , Citronenol, α-Terpineol, Methyl salycilate, Methyl Acetate, Citronolyl Acetate, Cineol, Linalol Perfumes such as Linalool, Ethyl Linalool, Vanillin, Thymol, Spearmint Oil, Sage Oil, Rosemary Oil, Cinamon Oil, etc. It can be used alone or in combination in an amount of 01 to 10%, preferably 0.5 to 5%, of the total composition.

In addition, sweeteners such as sodium saccharin, stevioside, aspartame, D-Xylose, etc. can be mixed and used in an amount of 0 to 1%, preferably 0.01 to 0.5%, of the total composition.

In addition, the oral composition of the present invention includes cationic disinfectants such as triclosan, chlorhexidine, benzethonium chloride, benzalkonium chloride, and cetylpyridium chloride, and alkali metal monofluorocarbons such as sodium monofluorophosphate and potassium monofluorophosphate. Fluorides such as phosphate, sodium fluoride, and stannous fluoride, tranexamic acid, epsilon amino caproic acid, aluminum chlorohydroxy allantoin, dihydrocholestanol, clitylic acid, glycerophosphatate, sodium chloride, and water-soluble inorganic phosphoric acid compounds. , ascorbic acid (sodium), dl-a-tocopherol acetate, xylitol, pyridoxine hydrochloride, polyvinyl pyrrolidone, etc., one or two or more of various effective ingredients can be selected and used.

Additionally, the surfactant used as a foaming component in the oral composition of the present invention may include an anionic surfactant, a nonionic surfactant, and/or a cationic surfactant.

Here, the anionic surfactant includes sodium alkyl sulfate such as sodium lauryl sulfate, sodium myristyl sulfate, sodium N-Lauroyl sarcosinate, and sodium N-myristyl sarcosinate. Sodium N-acyl sarcosinate, etc. can be used, and nonionic surfactants include sugar-derived fatty acid esters such as sugar fatty acid ester, maltose fatty acid ester, and lactose fatty acid ester, and poly Oxyethylene hydrogenated castor oil, polyoxyethylene higher alcohol ether, polyoxyethylene polyoxypropylene copolymer, polyoxyethylene polypropylene fatty acid ester, etc. can be used.

Cationic surfactants include N-alkyl diaminoethylglycine such as N-lauryl diaminoethylglycine, N-myristyl diaminoethylglycine, N-alkyl-N-carboxymethyl ammonium betaine, and 2-alkyl-1. -Hydroxyethyl imidazoline betaine sodium, etc. can be used.

In this case, anionic surfactants are mainly used as foaming ingredients, and sodium alkyl sulfate such as sodium lauryl sulfate is most widely used. In addition, these surfactants are preferably used singly or in combination of two or more, and the mixing amount is usually 0.01 to 0.5%, more preferably 0.05 to 0.3%, of the total composition.

The kidney extract may be included in an amount of 0.01 to 50% by weight based on the total weight of the oral composition of the present invention, but the content can be appropriately adjusted depending on the absorption of the active ingredient, the patient's age, gender, condition, degree of dental caries, etc. You can.

The term "comprising an active ingredient" in the present invention means that the extract contains a sufficient amount to achieve the efficacy or activity of the extract.

The Shinyi extract kills Streptococcus mutans bacteria that cause dental caries, inhibits their growth, inhibits the production of organic acids by mutans bacteria, and inhibits attachment to the tooth surface, so it can be used to prevent and improve oral diseases. You can.

The present invention provides a health functional food composition for preventing or improving dental caries containing a kidney extract as an active ingredient.

The term "comprising an active ingredient" in the present invention means that the extract contains a sufficient amount to achieve the efficacy or activity of the extract.

For administration, the health functional food composition of the present invention may contain 0.1 to 99.9% by weight of the extract of the present invention as an active ingredient based on the total weight of the composition. In addition, the composition of the present invention may further include one or more active ingredients that exhibit the same or similar functions in addition to the above-mentioned effective ingredients.

The term "health functional food" in the present invention refers to a product manufactured using nutrients that are easily deficient in daily diet or raw materials or ingredients (functional raw materials) with useful functions for the human body, which maintain the normal function of the human body or activate physiological functions. It refers to food that maintains and improves health through, but is not limited to, food.

The health functional food of the present invention can be manufactured by adding kidney extract in an appropriate effective concentration range according to various manufacturing methods known in the art.

The health functional food of the present invention can be manufactured in the form of powder, granules, tablets, capsules, or liquid.

Since the health functional food composition of the present invention has the effect of preventing or improving oral diseases, it can prevent the oral diseases and improve oral diseases that have already occurred by continuously ingesting it in an individual for the purpose of preventing or improving oral diseases. there is.

The health functional food is not particularly limited as long as it is intended for the prevention or improvement of oral diseases, and can be applied to any entity. For example, it can be applied to any entity, including non-human animals such as monkeys, dogs, cats, rabbits, guinea pigs, rats, mice, cows, sheep, pigs, goats, birds, or fish.

The present invention provides a feed additive composition for preventing or improving dental caries containing kidney extract as an active ingredient.

Specifically, the oral disease of the present invention may be dental caries.

The dental caries can be caused by Streptococcus mutans.

The term "feed additive" in the present invention refers to substances added to feed for the purpose of various effects such as supplementing nutrients and preventing weight loss, improving the digestibility of fiber in feed, improving oil quality, preventing reproductive disorders and improving conception rates, and preventing high temperature stress in the summer. it means.

The feed additive of the present invention has the effect of preventing or improving oral diseases, so it can prevent oral cardiovascular diseases and improve oral diseases that have already occurred by continuously ingesting it in poultry, livestock, etc.

When supplying the above feed additive, it can be supplied alone to poultry and livestock, or mixed with feed.

In addition, the feed additive of the present invention can be manufactured by adding kidney extract in an appropriate effective concentration range according to various feed production methods known in the art.

The additive is not particularly limited as long as it is intended for the prevention or improvement of oral diseases, and can be applied to any object. For example, it can be applied to any entity, including non-human animals such as monkeys, dogs, cats, rabbits, guinea pigs, rats, mice, cows, sheep, pigs, goats, birds, or fish.

The present invention includes the steps of 1) adding a solvent to the kidney to obtain an extract;

2) Concentrating the extract to obtain a concentrate; and

3) drying the concentrate.

The soybeans of the present invention may be, but are not limited to, the roots, stems, leaves or flowers of the axils.

The kidney extract of the present invention can be prepared by extraction with water, alcohol, or a mixture thereof.

The alcohol may be a C _1-2 lower alcohol, preferably ethanol, but is not limited thereto.

The alcohol may be 30 to 99% ethanol, 40 to 90% ethanol, 50 to 80% ethanol, and 60 to 75% ethanol, but is not limited thereto.

The extraction in step 1) may be performed at 90 to 100°C for 1 to 3 hours.

The concentration in step 2) may be reduced pressure concentration, but is not limited thereto.

The drying in step 3) may be spray drying, but is not limited thereto.

In a specific example, the present inventors added the Shinni extract to Streptococcus mutans bacteria and measured the absorbance after culturing for 24 hours to confirm the growth inhibitory activity and acid production inhibitory effect of the Shinni extract on dental caries-causing bacteria. As a result, , it was confirmed that the Shiny extract significantly inhibited the growth of Mutans bacteria (Figure 1), and it was confirmed that the Shiny extract inhibited the production of organic acids by Mutans bacteria (Table 1). In addition, as a result of confirming the antibacterial activity of the Shinni extract using the disk diffusion method, it was confirmed that the Shinni extract has an antibacterial effect that strongly inhibits the growth of mutans bacteria (Table 2). In addition, by confirming the possibility of inhibiting the adhesion of mutans bacteria to the tooth surface through the adhesion inhibitory effect on hydroxypatite beads (Figure 2), it was proven that the Shinyi extract of the present invention can be used for the treatment and prevention of dental caries.

Hereinafter, the present invention will be described in detail through the following examples.

However, the following examples are only for illustrating the present invention, and the present invention is not limited thereto.

<Example 1> Preparation of Xingi extract

In the present invention, fresh flower buds were extracted at 95°C for 2 hours by adding water equivalent to 8 times its weight. Afterwards, the extract was concentrated using a reduced pressure concentrator, and then the concentrated extract was spray dried to obtain 5 kg of solid Xingar extract.

<Experimental Example 1> Growth inhibitory activity and acid production inhibition test on dental caries-causing bacteria of Shingi extract

The strain used in this experiment was Streptococcus mutans KCTC 3065. Brain heart infusion agar (difco Lab, Detroit, MI, USA) was used as a growth medium for S mutans strains. The strain was inoculated to 1X10^8 CFU/mL after adding kidney extract to BHI liquid medium. After culturing in a thermostat at 37°C for 24 hours, light absorption was performed at 550 nm using an ELISA reader (Molecular Devices Co, CF, USA), and the pH was measured using a pH meter (ORIONSA 720, USA) to determine the acid production inhibition effect. observed.

In order to observe the antibacterial activity of the S. mutans extract prepared in Example 1, the S. mutans extract was added to the BHI liquid medium at a concentration of 0.125, 0.25, 0.5, and 1 mg/mL, and then inoculated with S. mutans. As a result of measuring the absorbance after culturing in a thermostat at 37°C for 24 hours, the control group without the kidney extract showed an absorbance of 0.198 ± 0.03. However, at concentrations of 0.5 and 1 mg/mL, the extract showed absorbance of 0.108 ± 0.021 and 0.075 ± 0.019, respectively, showing a significant concentration-dependent difference compared to the control group, showing a growth inhibitory effect (Figure 1).

In addition, in order to determine the effect of inhibiting the production of organic acids by Smutans according to the treatment of Sinicis extract, Smutans were inoculated into samples at concentrations of 0.125, 0.25, 0.5, and 1 mg/mL, and the pH was measured with a pH meter after incubation for 24 hours. It is listed in Table 1.

In the control group without kidney extract, the pH was 5.12 ± 0.04, at a concentration of 0.125 mg/ml it was 5.26 ± 0.01, and at a concentration of 0.25 mg/ml it was 5.19 ± 0.07, which was not a significant difference compared to the control group, but at a concentration of 0.5 mg/ml. Acid production was significantly suppressed compared to the control group at a concentration of 0.5 mg/ml or higher, with 5.57±0.02 at a concentration of 1 mg/ml and 6.49±0.05 at a concentration of 1 mg/ml (p<005).

Xinyi Extract Concentration (MKE Conc.) (mg/ml) control group 5.12±0.04 0.125 5.26±0.01 0.25 5.19±0.07 0.5 5.57±0.02* One 6.49±0.05*

<Experimental Example 2> Measurement of antibacterial activity of Shingi extract against bacteria causing dental caries

Disc diffusion method was used to measure the antibacterial effect of the Siniculi extract prepared in Example 1. The S mutans strain was grown in 20 mL of growth medium for about 18 hours, adjusted to an absorbance value of 0.5 at 620 nm, and the bacteria were evenly smeared on the entire surface of the medium while rotating with a smear rod. A sample of the kidney extract was prepared by dissolving it in sterilized distilled water to 50 mg/mL in a sterilized Eppendorf tube. Samples were sterilized paper discs; After 1mg was absorbed into 8mm (Advantec, Tokyo Roshi Kaisha Ltd, Tokyo, Japan), the paper disc was adhered to the plate medium, cultured in a thermostat at 37°C for 24 hours, and a growth inhibition zone (inhibitory zone, clear) around the paper disc was added. The antibacterial activity was confirmed by measuring the diameter of the zone).

To determine the antibacterial effect of the kidney extract, Smutans bacteria were cultured, and the kidney extract was treated. As a result, the antibacterial activity was checked 42 hours later. As a result, a growth inhibition ring of 35 mm was formed in the area treated with the kidney extract, and the diameter was When measured, it was confirmed that a growth inhibition ring of 35 mm was formed, strongly suppressing the growth of dental caries bacteria. The results are listed in Table 2.

Test strain god extract Zone of inhibition (mm) S. mutans 35

The diameter of the clear zone including the disc diameter of 8.0 mm.

<Experimental Example 3> Evaluation of the effect of Xingi extract on inhibiting attachment of dental caries-causing bacteria

Saliva preparation

Saliva was collected from a healthy adult male by stimulation with paraffin wax and secreted in a cooled beaker. The collected saliva was then centrifuged (Mega 17R, Hanil, Korea, 12,000 rpm, 4°C, 15 minutes) and the supernatant was separated. After taking, it was treated at 60°C for 30 minutes to inactivate the degrading enzyme, and then stored at -20°C before use.

S-HA attachment inhibition experiment

30 mg of Hydropatite beads (Bio-Rad Lab., U.S.A.) were washed five times with neutral water to remove small particles and dried at 37°C before use. 30 mg of dried hydroxypatite beads were treated with 1 mL of saliva at 37°C for 60 minutes, and the saliva was coated on the beads. Afterwards, S-HA was washed three times with 0.1 M potasium phosphate buffer (KPB, pH 7.0), and then the Siniga extract prepared in Example 1 was added at various concentrations, and S. mutans was added to 1X10^7 CFU/mL. Next, it was attached to S-HA for 90 minutes in a shaking incubator at 37°C. Afterwards, it was washed three times with 0.1M KPB (pH 7.0) and then an ultrasonic device (50 W, 30 seconds) was used to dislodge the bacteria attached to S-HA. Next, the bacterial solution was diluted, spread on a Mitis salivarius agar plate (Difco Laboratories, U.S.A.), incubated in a 37°C thermostat for 24 hours, and the number of colonies was counted. The control group was conducted without adding kidney extract.

Experiment result

To determine whether the kidney extract had an inhibitory effect on S. mutans adhesion to S-HA, CFU was measured after 24 hours of culture. At a concentration of 0.25 mg/mL, 179 ± 10.58 ( It was confirmed that adhesion to S. mutans was significantly inhibited compared to the control group at concentrations of 0.25 mg/mL or more of the kidney extract in CFU/mL, and adhesion inhibition rates of 23%, 41%, and 60% were observed, respectively, compared to the control group at each concentration (Figure 2).

As mentioned above, the antibacterial activity was explored to confirm the value of the kidney extract as an antibacterial agent against oral bacteria and as a preventive and therapeutic agent for dental caries. As a result of observing the growth inhibition effect on Smutans, which has the greatest impact on dental caries, the growth rate of S mutans showed a significant growth inhibition effect in the groups treated with kidney extract 0.5 and 1 mg/mL compared to the control group, and also 0.5 and 1 mg/mL. It was confirmed that organic acid production was suppressed in the mg/mL treatment group. In addition, the treatment with the renal extract showed high antibacterial activity (observation of 35 mm of growth-inhibiting rings), and the effect of inhibiting S.mutans adhesion at a concentration of 0.25 mg/mL or higher was confirmed, making it possible for the renal extract to be used as a treatment material for oral cariogenic bacteria and caries in the future. It was confirmed that it can be used as a preventive and therapeutic agent.

Claims (15)

A pharmaceutical composition for preventing or treating dental caries containing kidney extract as an active ingredient.
According to paragraph 1,
A pharmaceutical composition, wherein the bacteria causing dental caries is Streptococcus mutans or Streptococcus sobrinus.
According to paragraph 1,
The composition is a pharmaceutical composition, characterized in that it inhibits the growth of bacteria that cause dental caries.
According to paragraph 1,
The composition is a pharmaceutical composition, characterized in that it inhibits the production of organic acids by bacteria that cause dental caries.
According to paragraph 1,
A pharmaceutical composition, characterized in that the composition has antibacterial activity against bacteria that cause dental caries.
According to paragraph 1,
A pharmaceutical composition, characterized in that the composition has the activity of inhibiting tooth attachment of bacteria that cause dental caries.
An oral composition for preventing or improving dental caries containing kidney extract as an active ingredient.
In clause 7,
The formulation of the oral composition is characterized in that it is one or more formulations selected from the group consisting of toothpaste, mouthwash, mouthwash, gum, candy, oral spray, oral ointment, oral varnish, oral rinse, and gum massage cream. , oral composition.
A health functional food composition for preventing or improving dental caries containing kidney extract as an active ingredient.
A feed additive composition for preventing or improving dental caries containing kidney extract as an active ingredient.
1) Obtaining an extract by adding a solvent to the kidney;
2) Concentrating the extract to obtain a concentrate; and
3) Drying the concentrate.
According to clause 11,
A method for producing an extract of Xinyi, characterized in that the Xinyi is the root, stem, leaf or flower of Xinyi.
According to clause 11,
A method for producing Shinyi extract, wherein the solvent in step 1) is water, C1 to C2 lower alcohol, or a mixture thereof.
According to clause 11,
A method for preparing a Shinyi extract, characterized in that the extraction in step 1) is performed at 90 to 100 ° C for 1 to 3 hours.
According to clause 11,
A method for producing Shinyi extract, characterized in that the drying in step 3) is spray drying.