KR20240055117A - 연속식 배양에서의 세포-제어된 관류 - Google Patents

연속식 배양에서의 세포-제어된 관류 Download PDF

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KR20240055117A
KR20240055117A KR1020247012127A KR20247012127A KR20240055117A KR 20240055117 A KR20240055117 A KR 20240055117A KR 1020247012127 A KR1020247012127 A KR 1020247012127A KR 20247012127 A KR20247012127 A KR 20247012127A KR 20240055117 A KR20240055117 A KR 20240055117A
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perfusion
bioreactor
glucose
medium
culture
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Korean (ko)
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그레고리 월터 힐러
매튜 폴 객논
애나 마리아 오발
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베링거 인겔하임 인터내셔날 게엠베하
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Publication of KR20240055117A publication Critical patent/KR20240055117A/ko
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0062—General methods for three-dimensional culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/10—Perfusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02—Stirrer or mobile mixing elements
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/26—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pH
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/60—Buffer, e.g. pH regulation, osmotic pressure
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2511/00—Cells for large scale production

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Sustainable Development (AREA)
  • Cell Biology (AREA)
  • Analytical Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
KR1020247012127A 2015-05-29 2016-05-27 연속식 배양에서의 세포-제어된 관류 Pending KR20240055117A (ko)

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
US201562168297P 2015-05-29 2015-05-29
US62/168,297 2015-05-29
US201562199388P 2015-07-31 2015-07-31
US62/199,388 2015-07-31
US201562246774P 2015-10-27 2015-10-27
US62/246,774 2015-10-27
PCT/US2016/034570 WO2016196261A1 (en) 2015-05-29 2016-05-27 Cell-controlled perfusion in continuous culture
KR1020177034457A KR102658955B1 (ko) 2015-05-29 2016-05-27 연속식 배양에서의 세포-제어된 관류

Related Parent Applications (1)

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KR1020177034457A Division KR102658955B1 (ko) 2015-05-29 2016-05-27 연속식 배양에서의 세포-제어된 관류

Publications (1)

Publication Number Publication Date
KR20240055117A true KR20240055117A (ko) 2024-04-26

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KR1020247012127A Pending KR20240055117A (ko) 2015-05-29 2016-05-27 연속식 배양에서의 세포-제어된 관류
KR1020177034457A Active KR102658955B1 (ko) 2015-05-29 2016-05-27 연속식 배양에서의 세포-제어된 관류

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Country Status (8)

Country Link
US (3) US11597903B2 (https=)
EP (2) EP3303558B1 (https=)
JP (2) JP7340324B2 (https=)
KR (2) KR20240055117A (https=)
CN (1) CN107922919A (https=)
AU (2) AU2016271607C1 (https=)
CA (1) CA2985702A1 (https=)
WO (1) WO2016196261A1 (https=)

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US10704021B2 (en) 2012-03-15 2020-07-07 Flodesign Sonics, Inc. Acoustic perfusion devices
US9950282B2 (en) 2012-03-15 2018-04-24 Flodesign Sonics, Inc. Electronic configuration and control for acoustic standing wave generation
US10967298B2 (en) 2012-03-15 2021-04-06 Flodesign Sonics, Inc. Driver and control for variable impedence load
US9458450B2 (en) 2012-03-15 2016-10-04 Flodesign Sonics, Inc. Acoustophoretic separation technology using multi-dimensional standing waves
WO2015105955A1 (en) 2014-01-08 2015-07-16 Flodesign Sonics, Inc. Acoustophoresis device with dual acoustophoretic chamber
US11377651B2 (en) 2016-10-19 2022-07-05 Flodesign Sonics, Inc. Cell therapy processes utilizing acoustophoresis
US11021699B2 (en) 2015-04-29 2021-06-01 FioDesign Sonics, Inc. Separation using angled acoustic waves
US11708572B2 (en) 2015-04-29 2023-07-25 Flodesign Sonics, Inc. Acoustic cell separation techniques and processes
US11459540B2 (en) 2015-07-28 2022-10-04 Flodesign Sonics, Inc. Expanded bed affinity selection
US11474085B2 (en) 2015-07-28 2022-10-18 Flodesign Sonics, Inc. Expanded bed affinity selection
KR20240173213A (ko) 2016-01-26 2024-12-10 베링거 인겔하임 인터내셔날 게엠베하 연결된 관류 대 연속-유동식 교반-탱크 반응기 세포 배양 시스템
US11085035B2 (en) 2016-05-03 2021-08-10 Flodesign Sonics, Inc. Therapeutic cell washing, concentration, and separation utilizing acoustophoresis
US11214789B2 (en) 2016-05-03 2022-01-04 Flodesign Sonics, Inc. Concentration and washing of particles with acoustics
WO2018075830A1 (en) 2016-10-19 2018-04-26 Flodesign Sonics, Inc. Affinity cell extraction by acoustics
US11609120B2 (en) 2017-10-06 2023-03-21 Lonza Ltd Automated control of cell culture using Raman spectroscopy
EP4039786A1 (en) 2017-10-16 2022-08-10 Regeneron Pharmaceuticals, Inc. Perfusion bioreactor and related methods of use
KR102439221B1 (ko) 2017-12-14 2022-09-01 프로디자인 소닉스, 인크. 음향 트랜스듀서 구동기 및 제어기
IL258738B (en) * 2018-04-16 2020-04-30 Pluristem Ltd Methods and compositions for formulating and dispensing pharmaceutical formulations
JP7231355B2 (ja) 2018-08-22 2023-03-01 日機装株式会社 細胞培養方法および細胞培養装置
WO2020086408A1 (en) 2018-10-26 2020-04-30 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services A high-yield perfusion-based transient gene expression bioprocess
CN110117527A (zh) * 2019-05-15 2019-08-13 刘宝全 一种干细胞代谢废物的强化排出方法
CN113924355B (zh) * 2019-05-28 2024-04-02 上海药明生物技术有限公司 用于监测和自动控制灌流细胞培养的拉曼光谱集成灌流细胞培养系统
MX2021014644A (es) * 2019-06-13 2022-04-06 Amgen Inc Control de perfusion basado en biomasa automatizado en la fabricacion de productos biologicos.
WO2021097281A1 (en) * 2019-11-15 2021-05-20 Lonza Ltd Process and system for producing an inoculum
US20240101955A1 (en) * 2020-12-04 2024-03-28 National University Corporation Tokai National Higher Education And Research System Continuous production method of microorganisms that do not require sterilization and system therefor
EP4026892A1 (en) * 2021-01-12 2022-07-13 Sartorius Stedim Biotech GmbH Device assembly and method for controlling an integrated continuous pharmaceutical or biopharmaceutical manufacturing process
JPWO2023190829A1 (https=) * 2022-03-31 2023-10-05
WO2025124527A1 (zh) * 2023-12-15 2025-06-19 南京蓬勃生物科技有限公司 细胞稳态灌流培养的方法和系统

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Publication number Publication date
KR20180010202A (ko) 2018-01-30
WO2016196261A1 (en) 2016-12-08
JP7340324B2 (ja) 2023-09-07
EP3303558A1 (en) 2018-04-11
US20250059486A1 (en) 2025-02-20
AU2016271607A1 (en) 2017-11-30
US20180171279A1 (en) 2018-06-21
EP4148121A2 (en) 2023-03-15
EP4148121A3 (en) 2023-06-07
CA2985702A1 (en) 2016-12-08
KR102658955B1 (ko) 2024-04-22
AU2022259788A1 (en) 2022-12-01
US12173267B2 (en) 2024-12-24
AU2016271607B2 (en) 2022-07-28
US20230242857A1 (en) 2023-08-03
JP2022050540A (ja) 2022-03-30
US11597903B2 (en) 2023-03-07
CN107922919A (zh) 2018-04-17
EP3303558B1 (en) 2022-05-25
JP7579289B2 (ja) 2024-11-07
JP2018516079A (ja) 2018-06-21
AU2016271607C1 (en) 2023-06-29

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