KR20240042828A - A method of manufacturing complex distilled extracts of natural substances as active ingredients - Google Patents

Abstract

The present invention relates to a method for producing a complex distilled extract of active ingredients from natural materials, and more specifically, the steps of producing a hot water extract of Cudrania leaf/stem by extracting Cudrania leaf/stem with hot water; Preparing a distilled extract of the Cudrania tree using a distillation extraction facility that extracts a liquid extract from the Cudrania tree by distillation; Preparing a Cudrania tree distillation-electrolysis extract by electrolyzing a distillation extract of the Cudrania tree obtained using a distillation extraction equipment for extracting a liquid extract from the Cudrania tree by distillation; And mixing 1 part by weight of the Cudrania leaf/stem hot water extract prepared in the above step, 2.5 to 5.5 parts by weight of the distilled Cudrania tree extract prepared in the above step, and 0.1 to 0.5 parts by weight of the distilled-electrolyzed extract of the Cudrania tree prepared in the above step. Including the step of manufacturing a complex distilled extract of active ingredients from natural materials,
The step of preparing the distilled extract of the Cudrania tree involves heating a container containing the Cudrania tree, including leaves, outposts, and soft woody branches of the Cudrania tree with stems with an average diameter of 7 cm or less, at 420 to 420 degrees Celsius. Heating at a temperature of 500°C for 1 to 2 hours, indirectly heating at a temperature of 60 to 80°C for 3 to 4 hours to obtain water vapor escaping from the Cudrania tree and evaporating, and cooling the water vapor to a temperature of 3 to 10°C. Step 1 of producing an extract by condensing; Step 2 of producing a redistilled extract by concentrating the extract at 0.7 to 0.9 atm and 65 to 75°C for 1 to 2 hours to obtain evaporated water vapor and cooling and condensing the extract to a temperature of 3 to 10°C; And a third step of preparing a Cudrania tree distillation extract by repeating step 2 of the re-distilled extract 2 to 5 times,
The step of preparing the Cudrania tree distillation-electrolysis extract involves using a heating container containing the Cudrania tree leaves, outposts, and soft woody Cudrania tree branches with stems with an average diameter of 7 cm or less. Heating at a temperature of 420 to 500 ℃ for 1 to 2 hours, and indirectly heating at a temperature of 60 to 80 ℃ for 3 to 4 hours to obtain water vapor that escapes from the Cudrania tree and evaporates, and heats the vapor to a temperature of 3 to 10 ℃. Step 1 of producing an extract by cooling and condensing to temperature; Step 2 of preparing an electrolyzed extract by adding the cooled and condensed extract to an electrolyte solution with a concentration of 0.25 to 0.5 M and electrolyzing it at a glow discharge voltage of 10 to 280 V for 10 to 90 minutes; And a third step of indirectly heating the electrolyzed extract at a temperature of 90 to 100 ° C. for 30 minutes to 1 hour to obtain evaporating water vapor and cooling and condensing it to a temperature of 3 to 10 ° C. to prepare a distillation-electrolyzed extract. including,
Using a distillation extraction facility that extracts liquid extracts using a distillation method, complex distilled extracts of active ingredients from natural materials are concentrated with high purity. However, they are non-toxic and can be used without burdening the scent, and can be used to treat various respiratory diseases such as asthma and bronchitis. This relates to a method of manufacturing a complex distilled extract of active ingredients from natural materials that has an improving effect on skin diseases such as atopy.

Description

{A method of manufacturing complex distilled extracts of natural substances as active ingredients}

The present invention contains a complex distilled extract of active ingredients from natural materials manufactured using a distillation extraction facility that extracts liquid extracts by distillation, and can be used without burden of skin irritation or scent, and improves respiratory diseases and atopic dermatitis. This relates to a method of manufacturing a highly effective complex distilled extract of natural ingredients and active ingredients.

Atopy or atopic syndrome refers to an allergic reaction in which the body becomes extremely sensitive to allergens without direct contact. The cause is not specifically known, and factors such as family history, gender, body mass index, region of residence, etc. It is known to be a multifactorial disease caused by complex factors such as pets, age of birth, and socioeconomic factors.

Looking at the mechanism of atopic allergy, when an allergen invades through the stratum corneum and reaches the epidermal cells, macrophages, a type of white blood cell, phagocytose it and break it down into pieces. If the amount of invading allergen is too large and the macrophage cannot handle it all, information about the presence of the allergen that has not yet been processed is sent to T cells, a type of white blood cell (which has the function of regulating immunity promotion and suppression). The cells then transfer the information to eosinophils (eosinophilic white blood cells), a type of white blood cell, and gather the eosinophils widely distributed in the blood to the site where the allergen has invaded. At this time, eosinophils accumulated in the affected area release excessive chemical transmitters (active oxygen species), causing inflammation, which is the cause of allergic reactions.

The main symptoms of atopy include severe itching, dry skin, rash, oozing, scabs, and scaly skin (scaly skin). Above all, it is characterized by severe itching. Since the atopic constitution cannot be fundamentally cured, atopic dermatitis can be said to be a disease that is controlled by avoiding triggers and receiving appropriate treatment rather than aiming for a cure.

These atopic dermatitis treatment methods include a method using a therapeutic agent and a method of supplying skin moisture using a moisturizer; Pressure method to relieve atopic dermatitis by removing inhaled antigens such as house dust mites, pollen, cockroaches, and animal hair; How to avoid foods that cause major food antigens such as milk, eggs, and peanuts; Emotional stability, etc., and in case of severe atopic dermatitis and pruritus, ultraviolet light therapy, steroids, immunosuppressants, interferon, immunoglobulin, etc. are used.

However, steroids, immunosuppressants, and antihistamines used as treatments have side effects such as arrhythmia, hypertension, nephrotoxicity, and drug interactions, and recently developed immunosuppressants such as calcineurin inhibitors tacrolimus, FK506, and pimecrolimus are used in pediatric patients. It is reported to increase the incidence of leukemia, making treatment difficult. To reduce these side effects, functional natural cosmetics with excellent antibacterial, moisturizing, and anti-inflammatory effects, no side effects, and low cost are emerging as a new alternative.

Meanwhile, with the recent development of industrial economy, environmental pollution and changes in dietary habits, respiratory diseases such as asthma, acute and chronic bronchitis, and allergic rhinitis are rapidly increasing. There are some differences in the causes and symptoms of these respiratory diseases, and although the development of new drugs is progressing according to respiratory diseases, the effects are only temporary and the symptoms are worsening day by day, increasing the problem.

As mentioned above, in order to eliminate the side effects and resistance of synthetic chemical new drugs, efforts are being made to develop compositions to improve the treatment of respiratory diseases using medicinal plants to solve these problems with 100% natural ingredients, but the effectiveness is significantly lower than that of actual synthetic chemical new drugs. There was a problem.

Republic of Korea Patent Registration No. 10-1127927 Republic of Korea Patent Registration No. 10-2117520

The present invention was devised to solve the above-mentioned problems, and it uses extracts of natural ingredients manufactured using a distillation extraction facility that extracts liquid extracts by distillation, so that it can be used without the burden of skin irritation and scent, while preventing asthma and other diseases. The aim is to provide a method for manufacturing a complex distilled extract of active ingredients from natural materials, which has excellent improvement effects on various respiratory diseases such as bronchitis and atopic dermatitis.

The various problems to be solved by the present invention are not limited to the problems mentioned above, and other problems not mentioned will be clearly understood by those skilled in the art from the description below.

One embodiment of the present invention includes the steps of extracting Cudrania leaves/stem with hot water to produce a Cudrania leaf/stem hot water extract; Preparing a distilled extract of the Cudrania tree using a distillation extraction facility that extracts a liquid extract from the Cudrania tree by distillation; Preparing a Cudrania tree distillation-electrolysis extract by electrolyzing a distillation extract of the Cudrania tree obtained using a distillation extraction equipment for extracting a liquid extract from the Cudrania tree by distillation; And mixing 1 part by weight of the Cudrania leaf/stem hot water extract prepared in the above step, 2.5 to 5.5 parts by weight of the distilled Cudrania tree extract prepared in the above step, and 0.1 to 0.5 parts by weight of the distilled-electrolyzed extract of the Cudrania tree prepared in the above step. Including the step of manufacturing a complex distilled extract of active ingredients from natural materials,

The step of preparing the distilled extract of the Cudrania tree involves heating a container containing the Cudrania tree, including leaves, outposts, and soft woody branches of the Cudrania tree with stems with an average diameter of 7 cm or less, at 420 to 420 degrees Celsius. Heating at a temperature of 500 ℃ for 1 to 2 hours, indirectly heating at a temperature of 60 to 80 ℃ for 3 to 4 hours to obtain water vapor that escapes from the Cudrania tree and evaporate, and cooling the vapor to a temperature of 3 to 10 ℃. Step 1 of producing an extract by condensing; Step 2 of producing a redistilled extract by concentrating the extract at 0.7 to 0.9 atm and 65 to 75°C for 1 to 2 hours to obtain evaporated water vapor and cooling and condensing the extract to a temperature of 3 to 10°C; And a third step of preparing a Cudrania tree distillation extract by repeating step 2 of the re-distilled extract 2 to 5 times,

The step of preparing the Cudrania tree distillation-electrolysis extract involves using a heating container containing the Cudrania tree leaves, outposts, and soft woody Cudrania tree branches with stems with an average diameter of 7 cm or less. Heating at a temperature of 420 to 500 ℃ for 1 to 2 hours, and indirectly heating at a temperature of 60 to 80 ℃ for 3 to 4 hours to obtain water vapor that escapes from the Cudrania tree and evaporates, and heats the vapor to a temperature of 3 to 10 ℃. Step 1 of producing an extract by cooling and condensing to temperature; Step 2 of preparing an electrolyzed extract by adding the cooled and condensed extract to an electrolyte solution with a concentration of 0.25 to 0.5 M and electrolyzing it at a glow discharge voltage of 10 to 280 V for 10 to 90 minutes; And a third step of indirectly heating the electrolyzed extract at a temperature of 90 to 100 ° C. for 30 minutes to 1 hour to obtain evaporating water vapor and cooling and condensing it to a temperature of 3 to 10 ° C. to prepare a distillation-electrolyzed extract. Provides a method for manufacturing a complex distilled extract containing active ingredients from natural materials.

The step of preparing the Cudrania leaf/stem hot water extract includes hot air drying the washed Cudrania leaf/stem at a temperature of 55 to 65°C; Add 10 to 25 times the weight of water and 0.1 to 0.5 times the weight of the clitoria fermentation extract compared to the weight of the dried kkujippong leaves/stem, heat at a temperature of 100 to 120 ° C. for 0.5 to 1 hour, and then heat at 65 to 75 ° C. Preparing an aqueous extract by extracting at temperature for 3 to 5 hours; And it may include the step of heating the extract to 75 to 90 ° C and keeping it warm for 15 to 30 minutes to deactivate the enzyme.

The clitoria fermented extract is prepared by adding 8 to 18 times the weight of water compared to the weight of the clitoria flower and extracting the clitoria water extract for 1.5 to 3 hours at a pressure of 0.7 to 0.8 atm and a temperature of 85 to 105 ° C. It can be prepared by a method comprising the step of preparing a clitoria fermented extract by inoculating the prepared clitoria water extract with Lactobacillus delbrueckii.

The electrolyte solution of the distilled-electrolyzed extract of Cudrania mulberry tree is an aqueous solution of at least one organic acid selected from the group consisting of citric acid (C ₆ H ₈ O ₇ ), formic acid (HCOOH), acetic acid (CH ₃ COOH), and mixtures thereof. It is mixed with Thoria fermentation extract at a weight ratio of 1:0.05 to 0.3;

The clitoria fermented extract is prepared by adding 8 to 18 times the weight of water compared to the weight of the clitoria flower and extracting the clitoria water extract for 1.5 to 3 hours at a pressure of 0.7 to 0.8 atm and a temperature of 85 to 105 ° C. It can be prepared by a method comprising the step of preparing a clitoria fermented extract by inoculating the prepared clitoria water extract with Bifidobacterium longum.

The distillation extraction facility is a place where materials such as kkujippong trees, stems, and leaves are placed, heated, and distilled. The still body (13) is installed on a frame (12) and surrounded by a heater (10) and a fire brick (11). and a still (17), which is equipped with an evaporation vapor outlet (14) at the upper end and is composed of a still cover (16) that closes and opens the still body (13) in a structure that can be opened and closed by an actuator (15); The evaporated vapor inlet 19 is connected to the evaporated vapor outlet 14 on the side of the distiller 17 by the pipe piping 18, and the liquid extract inlet 21 of the tank 20 is connected to the piping 18. A condenser (23) equipped with a connected liquid extract outlet (22) and cooling the evaporated vapor to liquefy it using circulating water flowing therein; As a place to finally collect the liquid extract, the liquid extract inlet 21 at the upper part is connected to the liquid extract outlet 22 of the condenser 23 by a pipe piping 18, and the drain at the lower part for discharging the liquid extract. It includes a tank (20) equipped with (24) and a valve (41),

At the bottom of the still body 13, there is a means for recovering the liquid extract as well as the remaining evaporated vapor collected at the bottom of the still body 13 in addition to the evaporated vapor that mostly escapes through the top of the still 17. An outlet 42 is formed in the lower part of (13), and a liquid extract sub-container 46 is connected to the outlet 42 by a screw fastening structure, and is attached to the outer wall of the liquid extract sub-container 46. As the cooling water jacket 43 for cooling water circulation is installed, moisture and vapor discharged from the outlet 42 of the distiller body 13 enter the liquid extract sub-container 46, flowing through the cooling water jacket 43. As condensation occurs due to the temperature difference due to heat exchange with the cooling water, the liquid extract can collect inside the liquid extract sub-container 46, and at the bottom of the liquid extract sub-container 46, there is a sub outlet for recovery of the liquid extract ( 44) and a sub valve 45 are installed to recover the liquid extract, and a strainer 47 is installed at the extraction port 42 to prevent impurities from flowing into the liquid extract sub container 46. It could be.

The method for producing a complex distilled extract of active ingredients from natural materials according to an embodiment of the present invention is used by modern people who are exposed to the risk of various respiratory diseases such as asthma and bronchitis due to environmental pollution (fine dust, etc.), irregular lifestyle, and stress. It can be useful for.

In addition, the method for producing a complex distilled extract of natural active ingredients according to an embodiment of the present invention significantly reduces the inflammatory response and itching in patients with atopic dermatitis and has an excellent skin moisturizing effect, which can improve skin diseases such as atopic dermatitis. There is an advantage.

In addition, the method for producing a complex distilled extract of active ingredients from natural materials according to an embodiment of the present invention uses a complex distilled extract of active ingredients from natural materials concentrated to high purity using a distillation extraction facility that extracts a liquid extract by distillation. Since it is non-toxic, it has fewer side effects such as skin irritation and has a light scent, so it has the advantage of being easily applicable to various fields such as cosmetics and food.

1 is a schematic diagram showing the overall layout of a distillation extraction facility according to an embodiment of the present invention.
Figure 2 is a cross-sectional view showing a distiller in a distillation extraction facility according to an embodiment of the present invention.
Figure 3 is a cross-sectional view showing a condenser in a distillation extraction facility according to an embodiment of the present invention.
Figure 4 shows the results of measuring the ear thickness of a mouse according to the present invention.
Figure 5 shows the results of observing an ear tissue section stained according to the present invention using an optical microscope.
Figure 6 shows the results of inflammatory cytokine inhibition activity in atopic dermatitis-inducing area (ear tissue) according to the present invention.

Hereinafter, embodiments of the present invention will be described in detail. However, this is presented as an example, and the present invention is not limited thereby, and the present invention is only defined by the scope of the claims to be described later.

One embodiment of the present invention includes the steps of extracting Cudrania leaves/stem with hot water to prepare a Cudrania leaf/stem hot water extract; Preparing a distilled extract of the Cudrania tree using a distillation extraction facility that extracts a liquid extract from the Cudrania tree by distillation; Preparing a Cudrania tree distillation-electrolysis extract by electrolyzing a distillation extract of the Cudrania tree obtained using a distillation extraction equipment for extracting a liquid extract from the Cudrania tree by distillation; And mixing 1 part by weight of the Cudrania leaf/stem hot water extract prepared in the above step, 2.5 to 5.5 parts by weight of the distilled Cudrania tree extract prepared in the above step, and 0.1 to 0.5 parts by weight of the distilled-electrolyzed extract of the Cudrania tree prepared in the above step. It includes the step of producing a complex distilled extract of active ingredients from natural materials.

Hereinafter, each step of manufacturing the complex distilled extract of natural active ingredients according to the present invention will be described in more detail as follows.

The step of preparing a hot water extract of Cudrania leaf/stem according to an embodiment of the present invention includes hot air drying the washed Cudrania leaf/stem at a temperature of 55 to 65°C; Preparing a water extract by adding 10 to 25 times the weight of water compared to the dried Cudrania leaf/stem and extracting for 6 to 10 hours at 0.7 to 0.8 atmosphere pressure and temperature of 75 to 80 ° C.; and heating the extract to 75 to 90° C. and keeping it warm for 15 to 30 minutes to deactivate the enzyme.

The hot water extraction is a method commonly used in the art, but the present invention is a Cudrania leaf/stem hot water extract obtained under the above temperature and time conditions in order to increase the atopic dermatitis improvement effect and reduce the toxicity of the Cudrania leaf/stem hot water extract. It is desirable to use . In particular, it is desirable to maintain the stability of the hot water extract of Cudrania leaf/stem through an enzyme deactivation step as described above. The water used during the hot water extraction is preferably purified water.

In addition, in the step of preparing the Cudrania leaf/stem hot water extract, it is preferable to mix the clitoria fermentation extract with water as an extraction solvent at a certain ratio. Specifically, hot air drying the washed kkujippong leaves/stem at a temperature of 55 to 65°C; Add 10 to 25 times the weight of water and 0.1 to 0.5 times the weight of the clitoria fermentation extract compared to the weight of the dried Cudrania leaf/stem, heat at a temperature of 100 to 120 ° C. for 0.5 to 1 hour, and then heat at 65 to 75 ° C. Preparing an aqueous extract by extracting at temperature for 3 to 5 hours; And it may include the step of heating the extract to 75 to 90 ° C and keeping it warm for 15 to 30 minutes to deactivate the enzyme.

The clitoria serves to relieve inflammation and increase the healing rate of wounds. However, since the clitoria extract has a unique color and has limitations in processing when used in fields such as food and cosmetics, the present invention uses a fermented extract obtained by fermenting the clitoria extract using specific bacteria. The fermented extract reduces color expression compared to the solvent extract while simultaneously improving skin moisturizing power as well as antibacterial and anti-inflammatory effects.

The clitoria fermented extract is prepared by adding 8 to 18 times the weight of water compared to the weight of the clitoria flower and extracting the clitoria water extract for 1.5 to 3 hours at a pressure of 0.7 to 0.8 atm and a temperature of 85 to 105 ° C. It can be prepared by a method comprising the step of preparing a clitoria fermented extract by inoculating the prepared clitoria water extract with Lactobacillus delbrueckii.

The clitoria is a plant of the legume genus Clitoria of the bean (blister) order and its scientific name is Clitoria ternatea. It is native to Southeast Asia and is distributed in tropical and subtropical regions. In the present invention, the extract of clitoria can be used as a part of the plant, such as clitoria flowers, fruits, leaves, roots, etc., or extracts from whole plants. Considering extraction efficiency, etc., clitoria flowers are preferable. Additionally, it is okay to use commercially available products.

Lactobacillus delbrueckii used in the present invention is not particularly limited, but may be a bacterium used in public institutions or a mutant strain thereof, a bacterium isolated from nature, or a mutant strain thereof. The mutant strain may be a strain that has the ability to produce a fermented product showing the effect of the present invention.

At this time, the hot water treatment temperature and time, enzyme deactivation temperature and time, and fermentation treatment temperature and time are each in the optimal range for maximizing the yield of the hot water extract and the activity of the fermenting bacteria.

The clitoria fermented extract is preferably contained in a weight ratio of 0.1 to 0.5 times the weight of dried Cudrania leaf/stem. If the content is less than 0.1 times, the effect may be minimal, and if it exceeds 0.5 times, skin irritation increases. It can be applied to foods, cosmetics, etc. due to its color development and burdensome aroma and taste, which may limit its application to food and cosmetics.

In the step of producing a distilled extract of Cudrania tree according to an embodiment of the present invention, it is preferable to use the distillation extraction equipment shown in Figures 1 to 3 below, which extracts the liquid extract by distillation.

Specifically, FIG. 1 is a schematic diagram showing the overall layout of a distillation extraction facility according to an embodiment of the present invention, FIG. 2 is a cross-sectional view showing a distiller in a distillation extraction facility according to an embodiment of the present invention, and FIG. 3 is a schematic diagram showing the overall layout of a distillation extraction facility according to an embodiment of the present invention. This is a cross-sectional view showing a condenser in a distillation extraction facility according to an embodiment of.

As shown in Figures 1 to 3, the distillation extraction equipment extracts trees and leaves of natural materials such as Cudrania tree, Quercus tree, Hwangchil tree, Hovenia tree, Vitamin tree, bamboo, Ginkgo tree, Loquat tree, etc. It includes a distiller (17) as a means of distilling by adding it with water and heating it. It is preferable that the present invention is a natural material derived from the Cudrania tree.

The still 17 includes a still body 13 surrounded by a heater 10 and a firebrick 11 and supported on a frame 12, and a pipe pipe extending toward the condenser 23 at the upper end ( It consists of a still cover (16) equipped with an evaporation vapor outlet (14) to which 18) is connected.

Here, a portion of the pipe piping 18 connected to the evaporation vapor outlet 17 is made of a flexible pipe and can be deformed together when the still cover 16 is opened and closed, and thus the still cover 16 It is possible to exclude interference due to pipe piping during the opening and closing operation of .

The still body 13 is in the form of a circular container and may have a double body structure in which two stainless steel sheets with different thicknesses overlap to form an inner wall and an outer wall.

The red clay wall 34 may be applied to the outer wall of the still body 13 to a thickness of approximately 5 to 20 cm.

At this time, in order to allow the far-infrared rays generated from the red clay wall 34 to be irradiated to the wooden material (e.g., Cudrania mulberry) contained inside the container, a plurality of far-infrared ray irradiation holes (not shown) are penetrated through the inner and outer walls of the container. It is desirable to form

The still cover 16 serves to seal the still body 13 containing wood-like material, and is installed on the top of the still body 13 in a structure that can be opened and closed by rotating by a hinge portion 48. .

For this purpose, an actuator 15, for example, a cylinder, is installed on the side of the still body 13, and the rod of the cylinder installed in this way is connected to a cover arm 35 extending on the side of the still cover 16. .

Accordingly, when the actuator 15 is operated, the still cover 16 can be rotated about 90° using the hinge portion 48 to open or close the still body 13.

In addition, the still cover 16 is equipped with a safety valve 36 that opens when the internal temperature of the still body 13 is above a certain temperature, as well as a temperature sensor (not shown) that detects the internal temperature of the still body 13. do.

Accordingly, when the internal temperature of the still body 13 exceeds the preset temperature, for example, 400 to 450° C., the safety valve 36 opens, thereby preventing the internal pressure of the still body from becoming excessively high.

In this regard, it is preferable that the internal temperature of the still body 13 is maintained at a maximum of about 350 to 400 ° C.

In addition, the refractory bricks 11 are built in a structure that surrounds the wall and floor of the still body 13, and a space is formed between the refractory wall 11 and the still body 13. 37) is created.

In addition, a heater 10 is installed along the inner surface of the refractory brick 11, and at this time, the heater 10 is a heater that generates heat up to about 900 ℃ so that the internal temperature of the still body 13 is 350 to 400 C. It is desirable to maintain the temperature around ℃.

Here, the heater 10 can be controlled by a control box 38 that receives a signal from a temperature sensor and outputs a control signal.

In a preferred embodiment, in addition to the evaporated vapor that mostly escapes through the top of the still 17, the remaining evaporated vapor that collects inside the still 17, that is, at the bottom of the still 13, is stored at the bottom of the still 13. Of course, a liquid extract sub-container 46 is installed as a means of recovering the liquid extract, and the liquid extract sub-container 46 installed in this way also contains a liquid extract, for example, a liquid extract of the active ingredient such as sap of a Cudrania tree. can be obtained.

For this purpose, an outlet 42 is formed in the lower part of the still body 13, and a liquid extract sub-vessel 46 is connected to the outlet 42 formed in this way using a screw fastening structure.

In addition, a cooling water jacket 43 for circulation of cooling water is installed on the outer wall of the liquid extract sub-vessel 46, and accordingly, moisture and/or water vapor discharged from the outlet 42 of the distiller main body 13 is used in the liquid extract. When it enters the sub-container 46, condensation occurs due to the temperature difference due to the heat exchange effect with the coolant flowing through the coolant jacket 43, and eventually the liquid extract (sap) can also collect inside the liquid extract sub-container 46. do.

In addition, a sub outlet 44 and a sub valve 45 for recovery of the liquid extract are installed at the bottom of the liquid extract sub container 46, through which the liquid extract (sap) can be recovered.

Here, the unexplained symbol 47 represents a filter installed at the extract port 42 to prevent impurities from flowing into the liquid extract sub-container 46.

In addition, the distillation extraction equipment includes a condenser 23 as a means of cooling the evaporated vapor generated in the distiller 17 into a liquid extract.

At the lower end of the condenser 23, there is an evaporated vapor inlet 19 connected to the evaporated vapor outlet 14 on the side of the distiller 17 and a pipe piping 18, as well as a liquid extract inlet 21 of the tank 20. A liquid extract outlet (22) is formed connected to the side by a pipe (18).

Here, it is recommended to use at least one condenser 23, preferably two connected in series.

These condensers 23 are isolated from each other and cool the evaporated vapor using heat exchange between the evaporated vapor and circulating water (e.g., cooling water) flowing inside, and eventually obtain a liquid extract from the evaporated vapor cooled by heat exchange. It becomes possible.

For this purpose, the condenser 23 includes a condenser body 31, a tube 32, and a plurality of perforated plates 33, and the condenser body 31 has an evaporation vapor inlet 19 and a liquid extract outlet at the bottom. (22), and a circulating water inlet 29 and a circulating water outlet 30 at the upper and lower ends, respectively, and maintain an inclined position at a certain angle, and the tube 32 is connected to the condenser body 31. It is installed in a structure connected to the evaporation vapor inlet 19 and the liquid extract outlet 22 from the inside to provide a flow passage for evaporation vapor, and the perforated diaphragm 33 is located inside the condenser body 31 along the length of the condenser body. It is installed in a zigzag form while maintaining regular intervals along the direction, leading to a zigzag flow of circulating water.

Here, the evaporated vapor that enters the lower space of the condenser 23 through the evaporated vapor inlet 19 flows upward along the tube 32, is sent to the upper space of the condenser 23, and then continues along the tube 32. It is induced and exits through the liquid extract outlet (22).

At this time, while the evaporated vapor flows along the inside of the tube 32, heat exchange occurs with the circulating water, allowing it to cool and condense into a liquid state.

In addition, the circulating water, for example, cooling water, entering through the circulating water inlet 29 flows upward along the inside of the condenser 23 and exits through the circulating water outlet 30 after completing heat exchange with the evaporated vapor. .

At this time, the circulating water flowing from the bottom to the top inside the condenser 23 can show a zigzag flow depending on the arrangement characteristics of each perforated diaphragm 33, and ultimately the residence time of the circulating water flowing inside the condenser 23 can be secured for a long time.

In addition, the distillation extraction equipment includes a tank 20 as a means of finally collecting the liquid extract (e.g., active ingredient liquid extract such as sap of Cudrania mulberry tree material) provided from the condenser 23.

The tank 20 is a tank disposed toward the rear end of the condenser 23, and the tank 20 has a liquid extract at the upper end connected to a pipe 18 extending from the liquid extract outlet 22 of the condenser 23. An inlet 21 and a drain 24 at the lower end through which the liquid extract stored inside the tank 20 is discharged are formed.

In addition, a valve 41 is installed on the side of the tank 20 to discharge the liquid extract stored in the tank, and the valve 41 at this time is located at a certain height from the bottom of the tank 20 to It serves to discharge the liquid extract at the top, which has a high water level, among the liquid extracts.

In particular, the area where the liquid extract is finally obtained, that is, the area where the tank 20 belongs, includes a final collection bin 40 that collects the liquid extract at the higher level among the liquid extracts filled in the tank, and the liquid extract filled in the tank. A preliminary collection tank 39 is provided to collect liquid extract at a lower level, for example, liquid extract containing impurities such as residues during primary distillation.

Here, the final collection tank 40 can be placed on the side where the valve 41 is located, and the preliminary collection tank 39 can be placed on the side where the drain 24 is located, and the liquid extract collected in the final collection tank 40 The liquid extract (e.g., liquid extract filtered several times using a strainer) collected in the preliminary collection container 39 can be diluted at a predetermined ratio to make a final product.

In addition, the distillation extraction equipment inserts a material container 27 containing materials such as trees and leaves into the still body 13, or inserts a material container 27 containing carbon dioxide into the still body 13. A container lifting device (28) is included as a means of removing from the interior.

The vessel lifting device 28 consists of a hoist 25 installed on the top of the still 17, and a hoist post 26 installed on the frame 12 to support the hoist 25.

In addition, the material container 27 is made of a mesh-shaped container made of metal, etc., and can be hung on the hoist side through a traction medium such as a wire, hook, or chain.

Accordingly, when inputting materials such as trees and leaves or discharging carbon dioxide, the wooden container 27, which carries a large load, can be easily lifted up or lowered using the container lifting device 28, making the work safe and convenient. You can do it.

Accordingly, the process of extracting a liquid extract, for example, a liquid extract of an active ingredient such as sap of Cudrania tree material using the distillation extraction equipment configured as described above, is as follows.

First, open the still cover 16 using the actuator 15, then cut the wood of the material to be extracted into the inside of the still body 13, and then add a certain amount of water along with the stems and leaves.

Next, after closing the still cover 16, the control box 38 is operated to start the distillation extraction operation.

Next, the heat of the heater 10 generated by the operation of the operation button (not shown) of the control box 38 is conducted to the wall of the still body 13 through the red clay wall 34 to extract distillation inside the still body. The material being heated is heated.

At this time, the heat generated from the heater 10 is conducted through the space 37, so that the material in the still body 13 is carbonized by indirect heat.

Here, the material inside the still body 13, where moisture is evaporated and carbonized by being heated by the heat of the heater 10, is not transmitted directly to the still body 13, but is indirectly transmitted through the space portion 37. Therefore, the material inside the still body 13 is carbonized gradually rather than rapidly, making it possible to obtain high-quality carbide (charcoal).

Next, when the heat of the heater 10 is applied to the material contained inside the still body 13, moisture comes out of the wood, stems, leaves, etc. of the material and evaporates.

Next, the evaporated vapor collected inside the still body 13 escapes through the evaporated vapor outlet 14 under its own pressure, then flows along the pipe 18 and is sent to the condenser 23.

Next, the evaporated vapor entering the inside of the condenser 23 is condensed by heat exchange with the cooling water and changed into a liquid extract, and this liquid extract is sent to the tank 20 along the pipe 18.

Next, the liquid extract collected in the tank 20 is collected in the final collection container 39 and the preliminary collection container 40, and is continuously diluted at an appropriate ratio and then placed in a container such as a bottle or barrel and aged at low temperature for about 6 months or more. After final purification, it is packaged in containers such as bottles to make the final product.

In addition, residual vapor or moisture that fails to escape through the evaporation vapor outlet 14 of the still cover 16 and falls toward the bottom of the still body 13 is discharged through the outlet 42 and is discharged into the liquid extract sub-container. It is collected inside (46) and cooled at the same time to make a liquid extract. The liquid extract thus produced is recovered by opening the sub-valve (45) and then filtered several times to be collected in the preliminary collection tank (40). It is made into a product by diluting it with the extract in an appropriate ratio.

In addition, the carbide (charcoal) remaining inside the still body (13) can be collected by opening the still cover (16) and then shipped and sold as charcoal used for grilling meat or as an addition to animal feed through unit packaging. It becomes possible.

Specifically, the step of producing a distillation extract of the Cudrania tree using the distillation and extraction equipment according to the present invention involves using the leaves, outposts, and branches of the Cudrania tree with soft wood having stems with an average diameter of 7 cm or less. A heating container containing a Cudrania tree is heated at a temperature of 420 to 500 ° C. for 1 to 2 hours, and indirectly heated at a temperature of 60 to 80 ° C. for 3 to 4 hours to cause water vapor to escape from the Cudrania tree and evaporate. A first step of preparing an extract by cooling and condensing the water vapor to a temperature of 3 to 10° C.; Step 2 of producing a redistilled extract by concentrating the extract at 0.7 to 0.9 atm and a temperature of 65 to 75°C for 1 to 2 hours to obtain evaporated water vapor and cooling and condensing the extract to a temperature of 3 to 10°C; And step 3 of producing a distilled extract of Cudrania tree by repeating step 2 of the re-distilled extract 2 to 5 times.

The Cudrania tree used in the present invention can be extracted not only from leaves, but also from whole plants, stems, etc. In particular, the Kujimul tree stored in the heating container may include soft woody Kujimul tree branches having stems with an average diameter of 7 cm or less. These soft woody branches of the Cudrania mulberry tree may include not only the leaves of the Cudrania tree, but also the outposts and stems. As a result, not only can the Cudrania tree, which has soft wood, be actively used, but it can also be more effective in improving respiratory diseases and atopic dermatitis.

In addition, the present invention can produce a concentrated extract with high purity by performing a 2-step process of re-distilling the extract that has undergone the 1-step distillation process and a 3-step process of repeatedly re-distilling the re-distilled extract. It not only increases the effectiveness of improving respiratory diseases and atopic dermatitis, but also reduces the toxicity of Cudrania tree and at the same time reduces its unique scent, making it easier to apply to various fields such as cosmetics and food.

The step of producing a distillation-electrolysis extract of Cudrania tree according to an embodiment of the present invention is performed using the distillation extraction equipment of FIGS. 1 to 3 described above, and the method of using the distillation extraction equipment is the same as above. The explanation for this is omitted.

Specifically, the step of preparing the Cudrania tree distillation-electrolysis extract involves storing Cudrania tree leaves, outposts, and soft woody Cudrania tree branches with stems with an average diameter of 7 cm or less. The heating container is heated at a temperature of 420 to 500 ° C. for 1 to 2 hours, and indirectly heated at a temperature of 60 to 80 ° C. for 3 to 4 hours to obtain water vapor escaping from the Cudrania tree and evaporating, and the water vapor is heated at a temperature of 3 to 10 Step 1 of producing an extract by cooling and condensing to a temperature of ℃; Step 2 of preparing an electrolyzed extract by adding the cooled and condensed extract to an electrolyte solution with a concentration of 0.25 to 0.5 M and electrolyzing it at a glow discharge voltage of 10 to 280 V for 10 to 90 minutes; And a third step of indirectly heating the electrolyzed extract at a temperature of 90 to 100 ° C. for 30 minutes to 1 hour to obtain evaporating water vapor and cooling and condensing it to a temperature of 3 to 10 ° C. to prepare a distillation-electrolyzed extract. Includes.

As described above, the distillation-electrolysis extract of Cudrania tree according to the present invention is a two-step process of electrolyzing the extract distilled in the first step and a three-step process of re-distilling the electrolyzed extract to produce a distillation-electrolyzed extract. It is carried out as The electrolysis process decomposes the first-stage distilled extract into low-molecular-weight substances and dramatically reduces viscosity, thereby dramatically improving absorbency.

The electrolyte solution is not particularly limited to those commonly used in the art, but is specifically selected from the group consisting of citric acid (C ₆ H ₈ O ₇ ), formic acid (HCOOH), acetic acid (CH ₃ COOH), and mixtures thereof. An aqueous solution of one or more organic acids can be used.

In addition, the present invention can be used by mixing the clitoria fermentation extract with the organic acid aqueous solution at a weight ratio of 1: 0.05 to 0.3 to improve skin moisturization as well as antibacterial and anti-inflammatory properties. If the amount of the clitoria fermented extract used is less than 0.05 weight ratio, the effect may be minimal, and if it exceeds 0.3 weight ratio, skin irritation may increase and color may develop, which may limit its use as cosmetics and foods.

The clitoria fermented extract is prepared by adding 8 to 18 times the weight of water compared to the weight of the clitoria flower and extracting the clitoria water extract for 1.5 to 3 hours at a pressure of 0.7 to 0.8 atmosphere and a temperature of 85 to 105 ° C. It can be prepared by a method comprising the step of preparing a clitoria fermented extract by inoculating the prepared clitoria water extract with Bifidobacterium longum.

Bifidobacterium longum used in the present invention is not particularly limited, but may be a bacterium used in public institutions or a mutant strain thereof, a bacterium isolated from nature, or a mutant strain thereof. The mutant strain may be a strain that has the ability to produce a fermented product showing the effect of the present invention.

At this time, the hot water treatment temperature and time and the fermentation treatment temperature and time are each in the optimal range for maximizing the yield of the hot water extract and the activity of the fermenting bacteria.

In the step of preparing the complex distilled extract of the active ingredient of the natural material according to the present invention, each of the Cudrania leaf/stem hot water extract, Cudrania tree distilled extract, and Cudrania tree distilled-electrolyzed extract prepared in the above step are mixed at a specific ratio.

The distilled extract of Cudrania tree preferably contains 2.5 to 5.5 parts by weight based on 1 part by weight of Cudrania leaf/stem hot water extract. If the content is less than 2.5 parts by weight, the effect of improving atopy may be minimal, and the content is 5.5 parts by weight. If it is exceeded, there is a problem that the toxicity and scent reduction effects may be reduced. In addition, the distillation-electrolysis extract of Cudrania tree preferably contains 0.1 to 0.5 parts by weight based on 1 part by weight of Cudrania leaf/stem hot water extract, and if the content is less than 0.1 part by weight, the effects such as improvement in viscosity and water absorption are achieved. The improvement may be minimal, and if the content exceeds 0.5 parts by weight, there is a problem that processability may be reduced due to an increase in components with reduced viscosity.

The complex distilled extract of natural active ingredients prepared according to one embodiment of the present invention contains 1 to 10% by weight based on the total weight when used in foods, and 1 to 50% by weight based on the total weight when used in cosmetics. It is desirable to do so. If the content of the natural material active ingredient complex distillation extract is less than the above range, the effect may be minimal, and if it exceeds the above range, there is a problem in that the above-described improvement effect is no longer expressed, which may lower economic feasibility.

The method for producing a complex distilled extract of active ingredients from natural materials according to an embodiment of the present invention is used by modern people who are exposed to the risk of various respiratory diseases such as asthma and bronchitis due to environmental pollution (fine dust, etc.), irregular lifestyle, and stress. It can be useful for. In addition, it has the advantage of significantly reducing the inflammatory response and itching in atopic dermatitis patients and has an excellent skin moisturizing effect, thereby improving atopic dermatitis. In addition, using a distillation extraction facility that extracts liquid extracts through distillation, a highly pure and concentrated complex distilled extract of active ingredients from natural materials is used. It is non-toxic, has fewer side effects such as skin irritation, and has a light fragrance, making it suitable for use in cosmetics and cosmetics. It has the advantage of being easily applicable to various fields such as food.

Above, the present invention has been described in detail with preferred embodiments, but the present invention is not limited to the above embodiments, and various modifications may be made by those skilled in the art within the scope of the technical idea of the present invention. This is possible.

<Preparation Example 1> Preparation of Cudrania leaf/stem hot water extract

The washed Cudrania leaves/stem were dried with hot air at a temperature of 60°C, 20 times the weight of water compared to the weight of the dried Cudrania leaves/stem was added, heated at a temperature of 100℃ for 1 hour, and then heated at a temperature of 70℃ for 4 hours. An aqueous extract was prepared by extraction.

The prepared extract was heated to 80°C and kept warm for 30 minutes to deactivate the enzyme to prepare a hot water extract of Cudrania leaf/stem.

<Preparation Example 2> Preparation of Cudrania leaf/stem hot water extract (containing clitoria fermentation extract)

Preparation Example 1 was carried out in the same manner as above, but water extract was prepared by adding 20 times the weight of water and 0.3 times the weight of the clitoria fermentation extract compared to the weight of the dried Cudrania leaf/stem, and enzyme deactivation was performed to produce a hydrothermal extract of Cudrania leaf/stem. was manufactured.

At this time, the fermented clitoria extract was prepared by mixing 10 g of clitoria flowers with 100 g of water, treating the mixture at 0.7 atm and 95°C for 2 hours, and then cooling to 30°C. Afterwards, Lactobacillus delbrueckii NBRC3202 was inoculated to about 5×10 ⁶ cfu/g and fermented at 30° C. for 48 hours.

<Preparation Example 3> Preparation of Cudrania tree distillation extract

A Kujimul tree was prepared by mixing 100 parts by weight of logs with an average diameter of about 10 cm or more, 25 parts by weight of Kujimul tree leaves, and 25 parts by weight of soft woody Kujimul tree branches with stems with an average diameter of 7 cm or less. .

Using the distillation extraction equipment shown in Figures 1 to 3 below, a distillation extract of Cudrania tree was extracted. More specifically, washing the prepared Cudrania tree and placing it in the distiller 17 of the distillation extraction equipment, heating the internal temperature of the distiller 17 at a temperature of 480 ° C. for 1 hour and at a temperature of about 70 ° C. for 4 hours. After heating for 3 hours using a pulse-type indirect heating method in which the heating step was repeated, and then cooling with cooling water at 5° C., a distilled Cudrania tree extract was obtained.

The Cudrania tree is removed from the distiller (17), the obtained Cudrania tree extract is placed, and the inside of the distiller (17) is evaporated by low-temperature and low-pressure concentration at 0.8 atm, 70 ° C. for 1 hour to obtain water vapor evaporated at 5 ° C. By cooling with cooling water, a redistilled Cudrania tree extract was obtained.

The process of obtaining the re-distilled liquefied extract of Cudrania mulberry tree was repeated twice to obtain a distilled extract of Cudrania communis.

<Preparation Example 4> Preparation of Cudrania tree distillation extract

The same procedure as in Preparation Example 3 was carried out, but the process of obtaining the re-distilled liquefied extract of Cudrania mulberry was repeated 5 times to obtain a distilled extract of Cudrania communis.

<Preparation Example 5> Preparation of Cudrania tree distillation-electrolysis extract

A Kujimul tree was prepared by mixing 100 parts by weight of logs with an average diameter of about 10 cm or more, 25 parts by weight of Kujimul tree leaves, and 25 parts by weight of soft woody Kujimul tree branches with stems with an average diameter of 7 cm or less. .

Cudrania tree extract was extracted using the distillation extraction equipment shown in Figures 1 to 3 below. More specifically, washing the prepared Cudrania tree and placing it in the distiller 17 of the distillation extraction equipment, heating the internal temperature of the distiller 17 at a temperature of 480 ° C. for 1 hour and at a temperature of about 70 ° C. for 4 hours. After heating for 3 hours using a pulse-type indirect heating method in which the heating step was repeated, and then cooling with cooling water at 5° C., a distilled Cudrania tree extract was obtained.

Afterwards, two electrodes and a thermometer were fixed to a 500ml polypropylene beaker, and an electrolyzer consisting of a silicone stopper with a tube through which the generated gas could leak was inserted. At this time, a platinum wire with a diameter of 50 μm was used as an anode, and stainless steel with a thickness of 1 mm and an area of 10 × 30 mm was used as a cathode. 200 ml of the cooled and condensed extract was added to the electrolytic cell, citric acid (C ₆ H ₈ O ₇ ) was added to make the concentration 0.5 M, and the mixture was stirred with a magnetic bar. The two electrodes were connected to a direct current power supply, 150 V was applied, and the distilled Cudrania tree extract was electrolyzed to finally obtain a Cudrania chinensis distilled-electrolyzed extract.

<Preparation Example 6> Preparation of Cudrania tree distillation-electrolysis extract (containing clitoria fermentation extract)

Preparation Example 5 was carried out in the same manner, except that 200 ml of the cooled and condensed extract was added, and a mixture of citric acid (C ₆ H ₈ O ₇ ) at a weight ratio of 1 and clitoria fermentation extract at a weight ratio of 0.1 was added to obtain a concentration of 0.5 M. This was done and electrolyzed to finally obtain a distilled-electrolyzed extract of Cudrania tree.

At this time, the fermented clitoria extract was prepared by mixing 10 g of clitoria flowers with 100 g of water, treating the mixture at 0.7 atm and 95°C for 2 hours, and then cooling to 30°C. Afterwards, Bifidobacterium longum was inoculated to about 5×10 ⁶ cfu/g and fermented at 30° C. for 48 hours.

<Preparation Example 7> Preparation of Cudrania tree distillation-electrolysis extract (containing clitoria fermentation extract)

Preparation Example 5 was carried out in the same manner as above, except that 200 ml of the cooled and condensed extract was added, and an organic acid aqueous solution containing citric acid (C ₆ H ₈ O ₇ ) and acetic acid (CH ₃ COOH) mixed in equal weight ratios was added at a weight ratio of 1 and A mixture of the Thoria fermented extract at a weight ratio of 0.2 was added to a concentration of 0.5 M and subjected to electrolysis to finally obtain a distilled-electrolyzed extract of Cudrania tree.

At this time, the fermented clitoria extract was prepared by mixing 10 g of clitoria flowers with 100 g of water, treating the mixture at 0.7 atm and 95°C for 2 hours, and then cooling to 30°C. Afterwards, Bifidobacterium longum was inoculated to about 5×10 ⁶ cfu/g and fermented at 30° C. for 48 hours.

<Examples 1 to 3> and <Comparative Examples 1 to 3>

By stirring and mixing the ingredients and contents as shown in Table 1 below, a complex distilled extract of active ingredients from natural materials and a comparative extract were prepared.

Classification (part by weight) Example 1 Example 2 Example 3 Comparative Example 1 Comparative example 2 Comparative example 3 Cudrania complex distilled extract Cudrania leaf/stem thermal water extract Manufacturing Example 1
(One) Manufacturing example 2
(One) Manufacturing example 2
(One) Manufacturing Example 1
(One) - Manufacturing example 1
(One) Cudrania tree distilled extract Manufacturing Example 3
(3) Manufacturing example 3
(3) Manufacturing example 4
(5) - Manufacturing example 3
(3) Manufacturing example 3
(3) Cudrania tree distillation-electrolysis extract Manufacturing example 5
(0.3) Manufacturing example 6
(0.3) Manufacturing example 7
(0.3) - - -

<Experimental Example 1> Sensory test

Sensory evaluations such as taste and overall preference were measured for the complex distilled extracts of active ingredients from natural materials in Examples 1 to 3 and the comparative extracts in Comparative Examples 1 to 3, and the results are shown in Table 2 below. The above sensory evaluation was measured by having 40 adult men and women in their 30s to 50s (20 men and women each) with sensory discrimination ability and with more than 3 years of experience in the food-related field evaluate them.

[Assessment Methods]

1 point: The taste is very dry and bitter, has a lot of the unique taste and off-flavor of kkujippong, and the overall preference is not very good.

3 points: The taste is strong and bitter, has a unique taste and smell of kkujippong, and the overall preference is not good.

5 points: The taste is average, there is a slight taste and off-flavor unique to kkujippong, and the overall preference is average.

7 points: The taste is excellent, there is almost no taste or off-flavor characteristic of kkujippong, and the overall preference is good.

9 points: The taste is very excellent, there is no taste or off-flavor characteristic of kkujippong, and the overall preference is very good.

division taste already Off-flavor overall preference Example 1 9.5 9.2 9.3 9.3 Example 2 9.4 9.5 9.4 9.4 Example 3 9.6 9.8 9.6 9.7 Comparative Example 1 4.9 5.5 5.0 5.2 Comparative Example 2 5.7 5.6 5.1 5.5 Comparative Example 3 6.2 8.1 7.3 7.2

As shown in Table 2 above, in terms of taste, taste and off-flavor, and overall preference in the sensory test, the complex distilled extract of the natural material active ingredient of Examples 1 to 3 according to an embodiment of the present invention was compared to the comparative extract of Comparative Examples 1 to 3. It was confirmed that it was significantly superior compared to .

<Experimental Example 2> Bronchial inflammation inhibition test

In order to confirm whether the complex distilled extract of the natural material active ingredient of Examples 1 to 3 and the comparative extract of Comparative Examples 1 to 3 have an effect of suppressing pulmonary bronchial inflammation in an asthma and chronic obstructive respiratory disease model, antigen was administered to sensitized mice. An increase in leukocytes in the lung bronchioles caused by exposure was confirmed (Pharmacological Research, 2010, 61, 288~297; Biochemical Pharmacology, 2010, 79,888~896).

For this purpose, 0.2 ml of a 1:1 mixture of 10 ㎍ ovalbumin (Ovalbumin, OVA, Sigma) and aluminum hydroxide (4 mg, aluminum hydroxide, Alum, Pierce) was administered to BALB/c female mice (65 weeks old) at 0, 7, and 0. Sensitization was achieved by intraperitoneal administration on day 14. Eight and ten days after the final sensitization date, 10% ovalbumin was made into an aerosol using high-pressure compressed air and sprayed for 50 minutes to induce airway inflammation. Each test substance (400 mg/kg of freeze-dried product of each composition, bid) was administered orally twice a day in the morning and afternoon from the 21st to the 23rd after the first sensitization. 24 hours after the last oral administration, bronchoalveolar lavage was performed using 15 ml of phosphate buffer solution (pH 7.2), and bronchoalveolar lavage fluid was collected. The number of white blood cells in the washing liquid was counted using a hematology analyzer (Drew Scientific Inc, HEMAVET HV950FS, M-950HV). The results are shown in Table 3 below by calculating the lung bronchial inflammation inhibition rate (%) based on the white blood cell count of the negative control group (1% CMC [carboxymethyl cellulose] administered group).

division Pulmonary bronchial inflammation inhibition rate (%) Example 1 37 Example 2 39 Example 3 42 Comparative Example 1 11 Comparative Example 2 21 Comparative Example 3 29

As shown in Table 3 above, it was confirmed that the pulmonary bronchial inflammation inhibition rate of the complex distilled extract of natural material active ingredients of Examples 1 to 3 according to an embodiment of the present invention was significantly improved compared to the comparative extract of Comparative Examples 1 to 3. .

<Experimental Example 3> Sensory experiment for each item through survey

The complex distilled extracts of active ingredients from natural materials of Examples 1 to 3 and the comparative extracts of Comparative Examples 1 to 3 were tested on a total of 150 people (50 each for allergic rhinitis patients, smokers, asthma and bronchial diseases), 2 per day. One sachet was consumed 3 times a day for 3 months, and the effect of improving rhinitis, phlegm, asthma, and bronchial function (coughing frequency) was evaluated according to the degree felt by each person using the 9-point scoring method below. The results are shown in Table 4 below. indicated.

[Evaluation method: 9-point scoring method]

9: It has an effect close to a complete cure.

7: Significant improvement is good

5: There is some improvement

3: Little improvement effect

1: No improvement effect

division Rhinitis sputum asthma bronchial function
(coughing frequency) Example 1 7.1 7.3 7.2 7.4 Example 2 7.3 7.5 7.4 7.5 Example 3 7.5 7.6 7.5 7.8 Comparative Example 1 5.8 5.9 5.8 6.0 Comparative Example 2 6.0 6.5 6.3 6.3 Comparative Example 3 6.0 6.2 6.3 6.5

As shown in Table 4, in terms of improvement in rhinitis, phlegm, asthma, and bronchial function (coughing frequency), the complex distilled extracts of active ingredients from natural materials of Examples 1 to 3 were better than the comparative extracts of Comparative Examples 1 to 3. It was confirmed that satisfaction was excellent.

<Experimental Example 4>

1. Construction of TMA-induced atopy animal model

Experimental animals were purchased as 6-week-old female BALB/c mice, acclimatized for a week, and then randomly mixed into 6 groups of 8 each: TMA negative and positive control groups, Examples 1 to 3, and dexamethasone-administered group, which is the positive control group for distilled Cudrania extract. Atopic animal experiments were conducted separately for 4 weeks. For TMA sensitization and challenge, remove the hair on the back of the mouse, mix a solution of acetone and isopropyl myricate in a 4:1 volume to make the final concentration of TMA 5%, and then apply 60 μL of the TMA mixture to the back to apply TMA. Skin sensitization was performed. A TMA challenge was performed by applying 20 μL of 2% TMA to the right ear every three days from the 3rd to the 27th day after sensitization, and the change in ear thickness was measured using a digital gauge the day after ear application. For drug treatment, in the case of the TMA negative and positive control groups, 200 μL of sterilized saline was orally administered, and Examples 1 to 3 were administered orally at 200 μL each, and the positive control dexamethasone group was administered 3 mg/kg of dexamethasone in 200 μL of sterilized water. It was dissolved in physiological saline and administered orally. The growth conditions in the animal room were maintained at a temperature of 23 ± 2 ℃ and humidity of 50 ± 10%, and the light and dark cycles were adjusted at 12-hour intervals. During the experiment, food and water were consumed without restrictions, and animal experiments were approved by the ethics committee (KFRI-M-18030) and performed in accordance with our standard operating instructions.

2. Measurement of ear thickness using a digital dial gauge

The mouse ear thickness was measured using a digital dial gauge (HD647-301) to minimize experimenter's measurement error. Ear thickness was measured using a blind test 24 hours after TMA treatment, and the measured values were tested for statistical significance using a 2-way-ANOVA using the turkey test.

Ear thickness was measured using a digital dial gauge on the 4th, 7th, 10th, 13th, 16th, 19th, 22nd, 25th, and 28th day after applying TMA to the right ear, respectively, and compared with the TMA-induced group. Thus, it was confirmed that the increase in ear thickness was significantly suppressed in a concentration-dependent manner in the administration groups of Examples 1 to 3, and the results are shown in Figure 4. As shown in Figure 4, as a result of confirming the clinical symptoms of atopic dermatitis in ear tissue, no clinical symptoms were observed in the non-treated control group and the dexamethasone administered group, which is the positive control group of the Cudrania chinensis distilled extract, but when TMA was repeatedly applied, the number of applications and elapsed time Accordingly, severe symptoms of erythema and keratin were confirmed, and in the groups administered Examples 1 to 3, it was confirmed that clinical symptoms were suppressed in proportion to the administered concentration.

3. Observation of ear tissue sections

Ear tissue sections were fixed in 4% paraformaldehyde solution, sectioned, and stained with hematoxylin and eosin, and observed under an optical microscope. The results are shown in Figure 5. As a result of Figure 5, in the non-treated control group and the dexamethasone positive control group, the degree of infiltration and edema of immune cells that increased specifically for the disease could not be confirmed, and in the TMA-induced group, the thickness of the skin epidermis and dermis layer increased due to edema and keratinization and a large number of Immune cell infiltration and eosinophilic sponging of inflammatory tissue were confirmed. On the other hand, in the groups administered in Examples 1 and 2, the increase in the thickness of the skin tissue layer was reduced and the infiltration of immune cells was also suppressed compared to the TMA group. also. As a result of observing toluidine blue-stained tissue with an optical microscope, mast cells and mast cells in which degranulation had progressed could not be confirmed in the untreated control group, and normal mast cells were confirmed in the dexamethasone positive control group. In the TMA-induced group, an increase in mast cells and mast cells in which degranulation occurred as the disease progressed were confirmed, while in the Examples 1 and 2 administration groups, both mast cells and degranulation of mast cells were suppressed compared to the TMA group.

4. Inflammatory cytokine inhibition activity in atopic dermatitis-causing area (ear tissue)

To analyze the immunomodulatory effect of distilled extract of Kujippong in a TMA-induced atopic dermatitis model, RNA was extracted from ear tissue where lesions occurred and genes such as inflammatory markers such as IFNγ, IL6, IL1β, and CCL2, which are related to the infiltration and activity of inflammatory cells. The expression pattern was analyzed using a real-time gene amplification system, and the results are shown in Figure 6. It was confirmed that gene expression of inflammatory cytokines was significantly increased in the TMA-induced group compared to the non-treated control group and the dexamethasone-treated group, which was the positive control group in Example 1. Looking at the increase in the expression level of each gene, it was confirmed that IFNγ increased by about 1000 times, IL6 by about 60 times, and IL1β and CCL by about 170 times and 60 times, respectively, compared to the untreated control group. Example 1 administration group (Figure 4) The highest anti-inflammatory activity was confirmed (indicated by ☆).

Experimental Example 5: Evaluation of efficacy against atopic dermatitis

Sensory evaluation was performed on 16 healthy people (8 men, 8 women) aged 25 to 35 years old who were all concerned about dry skin but had normal skin rather than atopic dermatitis. Steroids, other antibiotics, and other moisturizing products were to be applied more than twice a day for 3 days while avoiding the use. Scores were given according to the following criteria, and qualitative evaluation was performed using the evaluation table below. The results are shown in Table 5 below.

<Evaluation criteria: Choose 1 from worsened/no difference/improved>

division Itching relief Improvement of skin tightness skin flexibility Example 1 worsened - - - no difference - 3 One improved 16 13 15 Example 2 worsened - - - no difference - One - improved 16 15 16 Example 3 worsened - - - no difference - - - improved 16 16 16 Comparative Example 1 worsened - - - no difference 16 16 13 improved - - 3 Comparative example 2 worsened - - - no difference 11 12 9 improved 5 4 7 Comparative Example 3 worsened - - - no difference 6 7 4 improved 10 9 12

As shown in Table 5 above, it was confirmed that many people felt that the comparative extracts of Comparative Examples 1 to 3 had no difference in reducing itchiness, improving skin tightness, or improving skin flexibility. On the other hand, most of the evaluators felt that the complex distilled extracts of active ingredients from natural materials in Examples 1 to 3 were improved in terms of reducing itching, improving skin tightness, or improving skin flexibility, and all of the evaluators felt that they had improved, especially in relieving itchiness. It was confirmed that this was done.

As described above, a person skilled in the art to which the present invention pertains will understand that the present invention can be implemented in other specific forms without changing its technical idea or essential features. Therefore, all embodiments described above should be understood as illustrative and not restrictive. The scope of the present invention should be construed as including all changes or modified forms derived from the meaning and scope of the claims described below and their equivalent concepts rather than the detailed description above.

10: heating container 11: body
12: outer wall 12a: inner wall
13: Red clay wall 14: Far infrared irradiation hole
15: mulberry oil extractor 16: strainer
17: storage part 18, 21: flange
19: fixing pin 19a: tightening nut
20: Lid 21a: Fixing groove
22: fundus cock 23: internal temperature sensing rod
30: mulberry oil storage container 31: coolant pump
32: Cooling water circulation space 33: Outlet
34: discharge valve 40: gas
41: Plate 42: Fireproof insulation brick
43: Space 44: Heater
45: High heat temperature sensing rod 46: Stainless steel cover
50: Control box 60: Carbon packing

Claims (5)

Preparing a Cudrania leaf/stem hot water extract by hot water extracting Cudrania leaves/stem;
Preparing a distilled extract of the Cudrania tree using a distillation extraction facility that extracts a liquid extract from the Cudrania tree by distillation;
Preparing a Cudrania tree distillation-electrolysis extract by electrolyzing a distillation extract of the Cudrania tree obtained using a distillation extraction equipment for extracting a liquid extract from the Cudrania tree by distillation; and
Natural product was obtained by mixing 1 part by weight of the Cudrania leaf/stem hot water extract prepared in the above step, 2.5 to 5.5 parts by weight of the distilled Cudrania tree extract prepared in the above step, and 0.1 to 0.5 parts by weight of the distilled-electrolyzed extract of the Cudrania tree prepared in the above step. Including the step of manufacturing a composite distilled extract of the active ingredient,
The step of preparing the Cudrania tree distillation extract is
A heating container containing the leaves, outposts, and soft woody branches of the Cudrania tree with stems with an average diameter of 7 cm or less is stored at a temperature of 420 to 500 ° C. for 1 to 2 hours. A first step of preparing an extract by heating and indirectly heating at a temperature of 60 to 80° C. for 3 to 4 hours to obtain water vapor evaporating from the Cudrania tree and cooling and condensing the water vapor to a temperature of 3 to 10° C.;
Step 2 of producing a redistilled extract by concentrating the extract at 0.7 to 0.9 atm and 65 to 75°C for 1 to 2 hours to obtain evaporated water vapor and cooling and condensing the extract to a temperature of 3 to 10°C; and
Step 3 of producing a distilled extract of Cudrania tree by repeating step 2 of the re-distilled extract 2 to 5 times;
The step of preparing the Cudrania tree distillation-electrolysis extract is
A heating container containing the leaves, outposts, and soft woody branches of the Cudrania tree with stems with an average diameter of 7 cm or less is stored at a temperature of 420 to 500 ° C. for 1 to 2 hours. A first step of preparing an extract by heating and indirectly heating at a temperature of 60 to 80° C. for 3 to 4 hours to obtain water vapor evaporating from the Cudrania tree and cooling and condensing the water vapor to a temperature of 3 to 10° C.;
Step 2 of preparing an electrolyzed extract by adding the cooled and condensed extract to an electrolyte solution with a concentration of 0.25 to 0.5 M and electrolyzing it at a glow discharge voltage of 10 to 280 V for 10 to 90 minutes; and
Step 3 of producing a distillation-electrolyzed extract by indirectly heating the electrolyzed extract at a temperature of 90 to 100 ° C. for 30 minutes to 1 hour to obtain evaporating water vapor and cooling and condensing it to a temperature of 3 to 10 ° C. A method for producing a complex distilled extract of active ingredients from natural materials, characterized in that:
According to paragraph 1,
The step of preparing the Cudrania leaf/stem hot water extract is
Hot air drying the washed kkujippong leaves/stem at a temperature of 55 to 65°C;
Add 10 to 25 times the weight of water and 0.1 to 0.5 times the weight of the clitoria fermentation extract compared to the weight of the dried Cudrania leaf/stem, heat at a temperature of 100 to 120 ° C. for 0.5 to 1 hour, and then heat at 65 to 75 ° C. Preparing an aqueous extract by extracting at temperature for 3 to 5 hours; and
A method for producing a complex distilled extract of an active ingredient based on natural materials, comprising the step of heating the extract to 75 to 90 ° C and keeping it warm for 15 to 30 minutes to deactivate the enzyme.
According to paragraph 2,
The clitoria fermentation extract is
Preparing a clitoria water extract by adding 8 to 18 times the weight of water compared to the weight of the clitoria flower and extracting the extract for 1.5 to 3 hours at a pressure of 0.7 to 0.8 atmosphere and a temperature of 85 to 105 ° C.
A method for producing a complex distilled extract of an active ingredient based on a natural product, characterized in that it is produced by a method comprising the step of producing a clitoria fermented extract by inoculating the prepared clitoria water extract with Lactobacillus delbrueckii.
According to paragraph 1,
The electrolyte solution of the distilled-electrolyzed extract of the Cudrania tree is
An aqueous solution of at least one organic acid selected from the group consisting of citric acid (C ₆ H ₈ O ₇ ), formic acid (HCOOH), acetic acid (CH ₃ COOH), and mixtures thereof is mixed with the clitoria fermentation extract in a weight ratio of 1:0.05 to 0.3. It is a mixture;
The clitoria fermentation extract is
Preparing a clitoria water extract by adding 8 to 18 times the weight of water compared to the weight of the clitoria flower and extracting the extract for 1.5 to 3 hours at a pressure of 0.7 to 0.8 atmosphere and a temperature of 85 to 105 ° C.
A method for producing a complex distilled extract of an active ingredient based on a natural product, characterized in that it is produced by a method comprising the step of producing a clitoria fermented extract by inoculating the prepared clitoria water extract with Bifidobacterium longum.
According to paragraph 1,
The distillation extraction equipment is
This is a place where materials such as kkujippong trees, stems, and leaves are placed, heated, and distilled. The distiller body 13 is installed on a frame 12 and surrounded by a heater 10 and a fire brick 11, and evaporates at the upper end. A still (17) equipped with a steam outlet (14) and composed of a still cover (16) that closes and opens the still body (13) in a structure that can be opened and closed by an actuator (15);
The evaporated vapor inlet 19 is connected to the evaporated vapor outlet 14 on the side of the distiller 17 by the pipe piping 18, and the liquid extract inlet 21 of the tank 20 is connected to the piping 18. A condenser (23) equipped with a connected liquid extract outlet (22) and cooling the evaporated vapor to liquefy it using circulating water flowing therein;
As a place to finally collect the liquid extract, the liquid extract inlet 21 at the upper part is connected to the liquid extract outlet 22 of the condenser 23 by a pipe piping 18, and the drain at the lower part for discharging the liquid extract. It includes a tank (20) equipped with (24) and a valve (41),
At the bottom of the still body 13, there is a means for recovering the liquid extract as well as the remaining evaporated vapor collected at the bottom of the still body 13 in addition to the evaporated vapor that mostly escapes through the top of the still 17. An outlet 42 is formed in the lower part of (13), and a liquid extract sub-container 46 is connected to the outlet 42 by a screw fastening structure, and is attached to the outer wall of the liquid extract sub-container 46. As the cooling water jacket 43 for cooling water circulation is installed, moisture and water vapor discharged from the outlet 42 of the distiller body 13 enters the liquid extract sub-container 46, flowing through the cooling water jacket 43. As condensation occurs due to the temperature difference due to heat exchange with the cooling water, the liquid extract can collect inside the liquid extract sub-container 46, and at the bottom of the liquid extract sub-container 46, there is a sub outlet for recovery of the liquid extract ( 44) and a sub valve 45 are installed to recover the liquid extract, and a strainer 47 is installed at the extraction port 42 to prevent impurities from flowing into the liquid extract sub container 46. A method for producing a complex distilled extract of active ingredients from natural materials.