KR20240042497A - 폴리펩타이드의 제작 방법, 태그, 발현 벡터, 폴리펩타이드의 평가 방법, 핵산 디스플레이 라이브러리의 제작 방법 및 스크리닝 방법 - Google Patents

폴리펩타이드의 제작 방법, 태그, 발현 벡터, 폴리펩타이드의 평가 방법, 핵산 디스플레이 라이브러리의 제작 방법 및 스크리닝 방법 Download PDF

Info

Publication number
KR20240042497A
KR20240042497A KR1020247007512A KR20247007512A KR20240042497A KR 20240042497 A KR20240042497 A KR 20240042497A KR 1020247007512 A KR1020247007512 A KR 1020247007512A KR 20247007512 A KR20247007512 A KR 20247007512A KR 20240042497 A KR20240042497 A KR 20240042497A
Authority
KR
South Korea
Prior art keywords
polypeptide
xaa
tag
lys
nucleic acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
KR1020247007512A
Other languages
English (en)
Korean (ko)
Inventor
겐 마츠이
조 도미나가
시오리 기하라
다카히로 호사카
다카요시 와타나베
Original Assignee
후지필름 가부시키가이샤
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 후지필름 가부시키가이샤 filed Critical 후지필름 가부시키가이샤
Publication of KR20240042497A publication Critical patent/KR20240042497A/ko
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K19/00—Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K5/00—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof
    • C07K5/04—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof containing only normal peptide links
    • C07K5/10—Tetrapeptides
    • C07K5/1002—Tetrapeptides with the first amino acid being neutral
    • C07K5/1005—Tetrapeptides with the first amino acid being neutral and aliphatic
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
    • C07K7/04—Linear peptides containing only normal peptide links
    • C07K7/06—Linear peptides containing only normal peptide links having 5 to 11 amino acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
    • C07K7/50—Cyclic peptides containing at least one abnormal peptide link
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/02—Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C40B40/08—Libraries containing RNA or DNA which encodes proteins, e.g. gene libraries
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/10—Libraries containing peptides or polypeptides, or derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Virology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
KR1020247007512A 2021-09-27 2022-09-26 폴리펩타이드의 제작 방법, 태그, 발현 벡터, 폴리펩타이드의 평가 방법, 핵산 디스플레이 라이브러리의 제작 방법 및 스크리닝 방법 Pending KR20240042497A (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JPJP-P-2021-157186 2021-09-27
JP2021157186 2021-09-27
PCT/JP2022/035787 WO2023048290A1 (ja) 2021-09-27 2022-09-26 ポリペプチドの作製方法、タグ、発現ベクター、ポリペプチドの評価方法、核酸ディスプレイライブラリの作製方法及びスクリーニング方法

Publications (1)

Publication Number Publication Date
KR20240042497A true KR20240042497A (ko) 2024-04-02

Family

ID=85720824

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020247007512A Pending KR20240042497A (ko) 2021-09-27 2022-09-26 폴리펩타이드의 제작 방법, 태그, 발현 벡터, 폴리펩타이드의 평가 방법, 핵산 디스플레이 라이브러리의 제작 방법 및 스크리닝 방법

Country Status (8)

Country Link
US (1) US20240247253A1 (https=)
EP (1) EP4410982A4 (https=)
JP (1) JPWO2023048290A1 (https=)
KR (1) KR20240042497A (https=)
CN (1) CN117858950A (https=)
AU (1) AU2022349179A1 (https=)
CA (1) CA3231403A1 (https=)
WO (1) WO2023048290A1 (https=)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPWO2024209857A1 (https=) * 2023-04-03 2024-10-10
WO2024214800A1 (ja) 2023-04-11 2024-10-17 富士フイルム株式会社 生理活性評価用のポリペプチド溶液を調製する方法、及びポリペプチドの生理活性を評価する方法
WO2025258399A1 (ja) * 2024-06-10 2025-12-18 富士フイルム株式会社 環状ペプチドのライブラリ、スクリーニング方法、及びペプチドの作製方法

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2004508801A (ja) * 1999-10-29 2004-03-25 カイロン　ソチエタ　ア　レスポンサビリタ　リミタータ ナイセリア抗原性ペプチド
WO2003089454A2 (en) * 2002-04-19 2003-10-30 California Institute Of Technology Unnatural amino acid containing display libraries
WO2008143679A2 (en) * 2006-06-01 2008-11-27 Verenium Corporation Nucleic acids and proteins and methods for making and using them
WO2010094288A1 (en) * 2009-02-20 2010-08-26 Aarhus Universitet Expressivity tag and use thereof
EP2797617B1 (en) * 2011-12-27 2020-01-22 Sorbonne Université Anti-tumor adjuvant therapy
JP6754997B2 (ja) * 2013-08-26 2020-09-16 国立大学法人 東京大学 大環状ペプチド、その製造方法、及び大環状ペプチドライブラリを用いるスクリーニング方法
US10141342B2 (en) 2014-09-26 2018-11-27 Semiconductor Energy Laboratory Co., Ltd. Semiconductor device and display device
WO2016156538A1 (en) * 2015-03-31 2016-10-06 Universite Pierre Et Marie Curie (Paris 6) Peptides that inhibit binding between set and caspase-9
JP6681625B2 (ja) 2015-06-16 2020-04-15 国立大学法人名古屋大学 タンパク質の発現方法

Also Published As

Publication number Publication date
EP4410982A4 (en) 2025-01-15
JPWO2023048290A1 (https=) 2023-03-30
US20240247253A1 (en) 2024-07-25
EP4410982A1 (en) 2024-08-07
AU2022349179A1 (en) 2024-03-07
CA3231403A1 (en) 2023-03-30
CN117858950A (zh) 2024-04-09
WO2023048290A1 (ja) 2023-03-30

Similar Documents

Publication Publication Date Title
JP6206943B2 (ja) ペプチドライブラリーの製造方法、ペプチドライブラリー、及びスクリーニング方法
KR20240042497A (ko) 폴리펩타이드의 제작 방법, 태그, 발현 벡터, 폴리펩타이드의 평가 방법, 핵산 디스플레이 라이브러리의 제작 방법 및 스크리닝 방법
JP6440055B2 (ja) ペプチドライブラリの製造方法、ペプチドライブラリ、及びスクリーニング方法
US20120208720A1 (en) Rapid display method in translational synthesis of peptide
US20180171321A1 (en) Platform for a non-natural amino acid incorporation into proteins
EP2952582A1 (en) Flexible display method
JP6357154B2 (ja) 荷電性非タンパク質性アミノ酸含有ペプチドの製造方法
US20240026343A1 (en) Method for screening dimerized cyclic peptide
US20220073908A1 (en) Methods of producing high diversity peptide libraries and promoting protein folding
WO2023048291A1 (ja) ｔＲＮＡ、アミノアシルｔＲＮＡ、ポリペプチド合成用試薬、非天然アミノ酸の導入方法、ポリペプチドの作製方法、核酸ディスプレイライブラリの作製方法、核酸－ポリペプチド連結体及びスクリーニング方法
US9783800B2 (en) Method for producing peptides having azole-derived skeleton
JP6332965B2 (ja) アゾリン化合物及びアゾール化合物のライブラリー、並びにその製造方法
WO2025258399A1 (ja) 環状ペプチドのライブラリ、スクリーニング方法、及びペプチドの作製方法
JP4897265B2 (ja) 新規な非天然アミノ酸のタンパク質への導入法
WO2024214800A1 (ja) 生理活性評価用のポリペプチド溶液を調製する方法、及びポリペプチドの生理活性を評価する方法
WO2024209857A1 (ja) 核酸ディスプレイライブラリの作製方法、スクリーニング方法、及びポリペプチドの作製方法
TW202613317A (zh) 環肽的庫、篩選方法及肽之製作方法
WO2012161227A1 (ja) 核酸構築物、核酸－蛋白質複合体、及びその利用
WO2006095474A1 (ja) 非天然アミノ酸及び／又はヒドロキシ酸含有化合物の製造方法

Legal Events

Date Code Title Description
PA0105 International application

St.27 status event code: A-0-1-A10-A15-nap-PA0105

PA0201 Request for examination

St.27 status event code: A-1-2-D10-D11-exm-PA0201

PG1501 Laying open of application

St.27 status event code: A-1-1-Q10-Q12-nap-PG1501

D13 Search requested

Free format text: ST27 STATUS EVENT CODE: A-1-2-D10-D13-SRH-X000 (AS PROVIDED BY THE NATIONAL OFFICE)

D13-X000 Search requested

St.27 status event code: A-1-2-D10-D13-srh-X000

PE0902 Notice of grounds for rejection

St.27 status event code: A-1-2-D10-D21-exm-PE0902