KR20240019077A - 나노규모 반응 챔버 및 이를 사용하는 방법 - Google Patents

나노규모 반응 챔버 및 이를 사용하는 방법 Download PDF

Info

Publication number
KR20240019077A
KR20240019077A KR1020237038411A KR20237038411A KR20240019077A KR 20240019077 A KR20240019077 A KR 20240019077A KR 1020237038411 A KR1020237038411 A KR 1020237038411A KR 20237038411 A KR20237038411 A KR 20237038411A KR 20240019077 A KR20240019077 A KR 20240019077A
Authority
KR
South Korea
Prior art keywords
nanovial
cell
cells
cavity
interest
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
KR1020237038411A
Other languages
English (en)
Korean (ko)
Inventor
루테 조셉 데
쉘던 주
웨이-잉 쿠오
Original Assignee
파틸리온 바이오사이언스 코포레이션
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 파틸리온 바이오사이언스 코포레이션 filed Critical 파틸리온 바이오사이언스 코포레이션
Publication of KR20240019077A publication Critical patent/KR20240019077A/ko
Pending legal-status Critical Current

Links

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6854Immunoglobulins
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/585Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with a particulate label, e.g. coloured latex
    • G01N33/587Nanoparticles
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508Rigid containers without fluid transport within
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1075Isolating an individual clone by screening libraries by coupling phenotype to genotype, not provided for in other groups of this subclass
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804Nucleic acid analysis using immunogens
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • G01N21/645Specially adapted constructive features of fluorimeters
    • G01N21/6456Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458Fluorescence microscopy
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • G01N21/6486Measuring fluorescence of biological material, e.g. DNA, RNA, cells
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5047Cells of the immune system
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5302Apparatus specially adapted for immunological test procedures
    • G01N33/5304Reaction vessels, e.g. agglutination plates
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/554Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being a biological cell or cell fragment, e.g. bacteria, yeast cells
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966Animal cells
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B82NANOTECHNOLOGY
    • B82YSPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y15/00Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B82NANOTECHNOLOGY
    • B82YSPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y5/00Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/705Assays involving receptors, cell surface antigens or cell surface determinants
    • G01N2333/70503Immunoglobulin superfamily, e.g. VCAMs, PECAM, LFA-3
    • G01N2333/7051T-cell receptor (TcR)-CD3 complex
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2458/00Labels used in chemical analysis of biological material
    • G01N2458/10Oligonucleotides as tagging agents for labelling antibodies
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2570/00Omics, e.g. proteomics, glycomics or lipidomics; Methods of analysis focusing on the entire complement of classes of biological molecules or subsets thereof, i.e. focusing on proteomes, glycomes or lipidomes

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Hematology (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Wood Science & Technology (AREA)
  • Urology & Nephrology (AREA)
  • Pathology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Cell Biology (AREA)
  • General Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Nanotechnology (AREA)
  • Clinical Laboratory Science (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Dispersion Chemistry (AREA)
  • Toxicology (AREA)
  • Mycology (AREA)
  • Virology (AREA)
  • Medical Informatics (AREA)
  • Plant Pathology (AREA)
KR1020237038411A 2021-04-14 2022-04-13 나노규모 반응 챔버 및 이를 사용하는 방법 Pending KR20240019077A (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202163174847P 2021-04-14 2021-04-14
US63/174,847 2021-04-14
PCT/US2022/024589 WO2022221391A1 (en) 2021-04-14 2022-04-13 Nanoscale reaction chambers and methods of using the same

Publications (1)

Publication Number Publication Date
KR20240019077A true KR20240019077A (ko) 2024-02-14

Family

ID=83640692

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020237038411A Pending KR20240019077A (ko) 2021-04-14 2022-04-13 나노규모 반응 챔버 및 이를 사용하는 방법

Country Status (8)

Country Link
US (3) US20250264477A1 (https=)
EP (1) EP4323542A4 (https=)
JP (1) JP2024517624A (https=)
KR (1) KR20240019077A (https=)
CN (1) CN117480260A (https=)
AU (1) AU2022258444A1 (https=)
CA (1) CA3215070A1 (https=)
WO (1) WO2022221391A1 (https=)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3838268B1 (en) 2017-02-24 2023-05-10 The Regents of the University of California Particle-drop structures and methods for making and using the same
WO2022221391A1 (en) 2021-04-14 2022-10-20 Partillion Bioscience Corporation Nanoscale reaction chambers and methods of using the same
EP4532711A4 (en) * 2022-05-31 2026-04-29 Bio Rad Laboratories Inc HYDROFEL PROCESSES AND COMPOSITIONS FOR SEPARING BIOLOGICAL SAMPLES

Family Cites Families (99)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5583162A (en) 1994-06-06 1996-12-10 Biopore Corporation Polymeric microbeads and method of preparation
US6190702B1 (en) 1996-03-28 2001-02-20 Takeda Chemical Industries, Ltd. Sustained-released material prepared by dispersing a lyophilized polypeptide in an oil phase
KR100541753B1 (ko) 1999-07-27 2006-01-10 가부시키가이샤 시세이도 마이크로캡슐 및 그 제조방법
ATE410680T1 (de) 2001-05-26 2008-10-15 One Cell Systems Inc Sekretion von proteinen durch verkapselte zellen
GB0127564D0 (en) 2001-11-16 2002-01-09 Medical Res Council Emulsion compositions
EP2283917B1 (en) 2002-05-09 2021-12-15 The University of Chicago Device for pressure-driven plug transport and reaction
US7901939B2 (en) 2002-05-09 2011-03-08 University Of Chicago Method for performing crystallization and reactions in pressure-driven fluid plugs
GB2399084B (en) 2002-07-30 2007-01-31 Univ Liverpool Porous beads and method of production thereof
CA2513899C (en) 2003-01-29 2013-03-26 454 Corporation Methods of amplifying and sequencing nucleic acids
GB0311664D0 (en) 2003-05-21 2003-06-25 Univ Manchester Polymeric hollow nanospheres
WO2006078841A1 (en) 2005-01-21 2006-07-27 President And Fellows Of Harvard College Systems and methods for forming fluidic droplets encapsulated in particles such as colloidal particles
US7709544B2 (en) 2005-10-25 2010-05-04 Massachusetts Institute Of Technology Microstructure synthesis by flow lithography and polymerization
KR100762361B1 (ko) 2006-12-06 2007-10-02 (주)이엠엘에스아이 디지털 이미지 테스트 부재의 광원 밝기 균일화 장치 및방법과 이를 이용한 디지털 이미지 색상 테스트 장치 및방법
US9029085B2 (en) 2007-03-07 2015-05-12 President And Fellows Of Harvard College Assays and other reactions involving droplets
US20110033547A1 (en) 2007-07-06 2011-02-10 Aarhus Universitet Dehydrated chitosan nanoparticles
EP2195372A2 (en) 2007-09-05 2010-06-16 Sunstorm Research Corporation Highly porous, large polymeric particles and methods of preparation and use
US8252517B2 (en) 2008-07-18 2012-08-28 Massachusetts Institute Of Technology Stop flow interference lithography system
WO2010042943A1 (en) 2008-10-10 2010-04-15 Massachusetts Institute Of Technology Tunable hydrogel microparticles
ES2781572T3 (es) 2009-04-14 2020-09-03 Univ California Procedimiento de creación de materiales coloreados fijando estructuras ordenadas de nanopartículas de magnetita dentro de un medio sólido
US8703058B1 (en) 2009-09-09 2014-04-22 Sandia Corporation Microfluidic devices and methods including porous polymer monoliths
WO2011044179A1 (en) 2009-10-05 2011-04-14 The Regents Of The University Of California Extracranial implantable devices, systems and methods for the treatment of neuropsychiatric disorders
WO2011049965A1 (en) 2009-10-20 2011-04-28 The Regents Of The University Of California Incoherent lensfree cell holography and microscopy on a chip
US9057702B2 (en) 2010-12-21 2015-06-16 The Regents Of The University Of California Compact wide-field fluorescent imaging on a mobile device
CN103492062A (zh) 2011-02-25 2014-01-01 美利肯公司 胶囊及包含其的组合物
CN103608285B (zh) 2011-04-14 2016-07-06 加利福尼亚大学董事会 多功能纳米颗粒设计和应用
US8658430B2 (en) 2011-07-20 2014-02-25 Raindance Technologies, Inc. Manipulating droplet size
WO2013041983A1 (en) 2011-09-19 2013-03-28 Centre National De La Recherche Scientifique Microfluidic system
US20140230909A1 (en) 2011-09-30 2014-08-21 The Regents Of The University Of California Devices and methods for programming fluid flow using sequenced microstructures
AU2013302756C1 (en) 2012-08-14 2018-05-17 10X Genomics, Inc. Microcapsule compositions and methods
FR2996545B1 (fr) 2012-10-08 2016-03-25 Ecole Polytech Procede microfluidique de traitement et d'analyse d'une solution contenant un materiel biologique, et circuit microfluidique correspondant.
US20140127290A1 (en) 2012-11-08 2014-05-08 Ohio State Innovation Foundation Microcapsules Encapsulating Living Cells
CN105492627A (zh) 2013-03-15 2016-04-13 伯乐生命医学产品有限公司 关联靶的数字测定
WO2014186440A2 (en) 2013-05-14 2014-11-20 President And Fellows Of Harvard College Rapid production of droplets
KR101660376B1 (ko) 2013-07-15 2016-11-09 주식회사 싸이토젠 시료의 예비평가 및 세포 포획용 칩
KR102758333B1 (ko) 2013-08-28 2025-01-23 벡톤 디킨슨 앤드 컴퍼니 대량의 동시 단일 세포 분석
EP3049067A4 (en) 2013-09-24 2017-05-03 The Regents of the University of California Encapsulated sensors and sensing systems for bioassays and diagnostics and methods for making and using them
GB201317301D0 (en) 2013-09-30 2013-11-13 Linnarsson Sten Method for capturing and encoding nucleic acid from a plurality of single cells
CN113215221A (zh) 2014-04-08 2021-08-06 华盛顿大学商业中心 用于使用多分散小滴执行数字检定的方法和设备
US20150298091A1 (en) 2014-04-21 2015-10-22 President And Fellows Of Harvard College Systems and methods for barcoding nucleic acids
AU2015250034B2 (en) 2014-04-21 2019-06-20 President And Fellows Of Harvard College Systems and methods for barcoding nucleic acids
WO2015200541A1 (en) 2014-06-24 2015-12-30 Bio-Rad Laboratories, Inc. Digital pcr barcoding
CN106714854B (zh) 2014-07-17 2020-09-04 加利福尼亚大学董事会 用于生物医学应用的可控的自退火微凝胶颗粒
WO2016018678A1 (en) 2014-07-30 2016-02-04 The Regents Of The University Of California Methods for bacteriophage detection
WO2016100234A1 (en) 2014-12-15 2016-06-23 The Regents Of The University Of California Method and device for separation of particles and cells using gradient magnetic ratcheting
EP3253479B1 (en) 2015-02-04 2022-09-21 The Regents of The University of California Sequencing of nucleic acids via barcoding in discrete entities
US20160289769A1 (en) * 2015-03-30 2016-10-06 Verily Life Sciences Llc Methods for Combining Single Cell Profiling with Combinatorial Nanoparticle Conjugate Library Screening and In Vivo Diagnostic System
WO2016160844A2 (en) 2015-03-30 2016-10-06 Cellular Research, Inc. Methods and compositions for combinatorial barcoding
WO2017027838A1 (en) 2015-08-13 2017-02-16 President And Fellows Of Harvard College Microfluidic devices and systems for cell culture and/or assay
WO2017059367A1 (en) 2015-10-02 2017-04-06 The Regents Of The University Of California System and method for optical transient liquid molding of microparticles and uses for the same
WO2017087693A2 (en) 2015-11-18 2017-05-26 Massachusetts Institute Of Technology Customizable hydrogel microwell array
US10011872B1 (en) 2016-12-22 2018-07-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
EP4029939B1 (en) 2017-01-30 2023-06-28 10X Genomics, Inc. Methods and systems for droplet-based single cell barcoding
US12264411B2 (en) * 2017-01-30 2025-04-01 10X Genomics, Inc. Methods and systems for analysis
EP3838268B1 (en) 2017-02-24 2023-05-10 The Regents of the University of California Particle-drop structures and methods for making and using the same
US10329557B2 (en) * 2017-03-13 2019-06-25 Gigagen, Inc. Systems and methods for massively parallel combinatorial analysis of single cells
WO2019139650A2 (en) 2017-09-29 2019-07-18 The Regents Of The University Of California Method of generating monodisperse emulsions
US12221720B2 (en) * 2017-11-13 2025-02-11 The Broad Institute, Inc. Methods for determining spatial and temporal gene expression dynamics during adult neurogenesis in single cells
JP2021505157A (ja) * 2017-12-07 2021-02-18 マサチューセッツ インスティテュート オブ テクノロジー 単一細胞分析
CA3005385A1 (en) 2018-05-18 2019-11-18 Stoyan K. Smoukov A method for the preparation of particles with controlled shape and/or size
AU2019321593B2 (en) * 2018-08-17 2025-01-23 The Regents Of The University Of California Particle-containing droplet systems with monodisperse fluid volumes
US20210340596A1 (en) 2018-09-28 2021-11-04 Fluent Biosciences Inc. Target capture and barcoding in monodisperse droplets
US12384890B2 (en) 2018-10-18 2025-08-12 The Regents Of The University Of California Methods for fabricating modular hydrogels from macromolecules with orthogonal physico-chemical responsivity
EP3670667A1 (en) * 2018-12-19 2020-06-24 Paris Sciences et Lettres - Quartier Latin Identification of cognate pairs of ligands and receptors
US11845983B1 (en) * 2019-01-09 2023-12-19 10X Genomics, Inc. Methods and systems for multiplexing of droplet based assays
US20220233413A1 (en) 2019-05-07 2022-07-28 The Regents Of The University Of California Monodisperse emulsions templated by three-dimensional structured microparticles and methods of making the same
US20210215591A1 (en) 2020-01-13 2021-07-15 Fluent Biosciences Inc. Devices for generating monodisperse droplets from a bulk liquid
JP7730449B2 (ja) 2020-01-13 2025-08-28 イルミナ インコーポレイテッド 単一細胞遺伝子プロファイリングのための方法およびシステム
WO2021146187A1 (en) 2020-01-13 2021-07-22 Fluent Biosciences Inc. Emulsion based drug screening
WO2021146183A1 (en) 2020-01-13 2021-07-22 Fluent Biosciences Inc. Reverse transcription during template emulsification
WO2021146168A1 (en) 2020-01-13 2021-07-22 Fluent Biosciences Inc. Methods and systems for amplifying low concentrations of nucleic acids
CN115698282A (zh) 2020-01-13 2023-02-03 福路伦特生物科学公司 单细胞测序
WO2021155057A1 (en) * 2020-01-29 2021-08-05 Becton, Dickinson And Company Barcoded wells for spatial mapping of single cells through sequencing
US11866782B2 (en) 2020-03-16 2024-01-09 Fluent Biosciences Inc. Multi-omic analysis in monodisperse droplets
EP4142927A4 (en) 2020-04-30 2024-04-10 The Regents of the University of California SYSTEMS AND METHODS FOR GENERATING MULTIPHASIC DROPLETS TO FORM SHAPED PARTICLES AND USES THEREOF
US11851700B1 (en) * 2020-05-13 2023-12-26 10X Genomics, Inc. Methods, kits, and compositions for processing extracellular molecules
EP4182477A4 (en) 2020-07-15 2024-09-11 Fluent Biosciences Inc. MULTI-LEVEL LIGATION OLIGOS
CN116348613A (zh) * 2020-07-31 2023-06-27 新格元生物科技有限公司 固定细胞的高通量分析
US20230257732A1 (en) 2020-08-05 2023-08-17 The Regents Of The University Of California Degradable hollow shell particles for high-throughput screening and sorting of cells
LT4211260T (lt) * 2020-09-11 2025-06-25 New England Biolabs, Inc. Imobilizuotų fermentų taikymas nanoporų bibliotekos konstravimui
EP4240868A4 (en) 2020-11-03 2024-11-13 Illumina, Inc. METHODS AND SYSTEMS FOR DETECTING PATHOGENIC MICROBES IN A PATIENT
ES3063619T3 (en) 2020-11-03 2026-04-17 Illumina Inc Systems and methods for making sequencing libraries
CA3209578A1 (en) 2021-01-25 2022-07-28 Fluent Biosciences Inc. Emulsification with magnetic hydrogels
WO2022221391A1 (en) 2021-04-14 2022-10-20 Partillion Bioscience Corporation Nanoscale reaction chambers and methods of using the same
CA3220479A1 (en) 2021-05-18 2022-11-24 Fluent Biosciences Inc. Template particles with micropores and nanopores
CA3220477A1 (en) 2021-05-18 2022-11-24 Fluent Biosciences Inc. Perturbed genomic expression in pretemplated instant partitions
CA3220457A1 (en) 2021-05-18 2022-11-24 Fluent Biosciences Inc. Multi-omic analysis of extracellular vesicles in monodisperse droplets
EP4341398A4 (en) 2021-05-18 2025-02-19 Fluent Biosciences Inc. PRE-TEMPLATE INSTANT PARTITIONS FOR SCREENING
EP4341428A4 (en) 2021-05-18 2025-05-21 Illumina, Inc. PROCEDURE FOR PERFORMING DIGITAL PCR
WO2022251445A1 (en) 2021-05-28 2022-12-01 Fluent Biosciences Inc. Spatial transcriptomics in pips
US20230002807A1 (en) 2021-06-30 2023-01-05 Fluent Biosciences Inc. Methods and compositions for nucleic acid analysis
WO2023283408A1 (en) 2021-07-09 2023-01-12 Fluent Biosciences Inc. Devices for generating pre-templated instant partitions
JP2024524662A (ja) 2021-07-15 2024-07-05 フルーエント バイオサイエンシーズ インコーポレイテッド 単一細胞分析のための分散型ワークフロー
JP2024525724A (ja) 2021-07-15 2024-07-12 フルーエント バイオサイエンシーズ インコーポレイテッド Dnaコード化ライブラリーの予め鋳型化された即時パーティション化
WO2023091376A1 (en) * 2021-11-16 2023-05-25 Bio-Rad Laboratories, Inc. Methods and compositions for genotyping and phenotyping cells
EP4532711A4 (en) * 2022-05-31 2026-04-29 Bio Rad Laboratories Inc HYDROFEL PROCESSES AND COMPOSITIONS FOR SEPARING BIOLOGICAL SAMPLES
EP4544550A2 (en) 2022-06-23 2025-04-30 Illumina Inc. Scrnaseq analysis systems
US12534721B2 (en) 2022-10-06 2026-01-27 Illumina, Inc. Quantitative detection and analysis of moleculesip
US11976325B2 (en) 2022-10-06 2024-05-07 Fluent Biosciences Inc. Quantitative detection and analysis of molecules
WO2024137573A1 (en) 2022-12-20 2024-06-27 Fluent Biosciences Inc. Cell type annotation

Also Published As

Publication number Publication date
US20260103743A1 (en) 2026-04-16
EP4323542A4 (en) 2025-03-05
AU2022258444A1 (en) 2023-11-23
CN117480260A (zh) 2024-01-30
US20250264477A1 (en) 2025-08-21
CA3215070A1 (en) 2022-10-20
EP4323542A1 (en) 2024-02-21
JP2024517624A (ja) 2024-04-23
WO2022221391A1 (en) 2022-10-20
US12503723B1 (en) 2025-12-23

Similar Documents

Publication Publication Date Title
US12503723B1 (en) Nanoscale reaction chambers and methods of using the same
JP6169111B2 (ja) マイクロ流体工学を利用した複数の単一細胞の捕捉及び処理の方法、システム、並びにデバイス
JP5241678B2 (ja) 微小流体粒子分析システム
CN101939649B (zh) 从复杂样品基质中对细胞进行分离和计数
CA2717070C (en) Gel microdrop composition and method of using the same
CN107110854B (zh) 分析液滴内容物的方法及相关装置
Van Lent et al. Miniaturized single-cell technologies for monoclonal antibody discovery
US20230323433A1 (en) Methods of Assaying a Biological Cell
JP7749687B2 (ja) 細胞を選択する方法
De Rutte et al. Massively parallel encapsulation of single cells with structured microparticles and secretion-based flow sorting
Koo et al. Sorting single T cells based on secreted cytokines and surface markers using hydrogel nanovials
US20230173449A1 (en) Systems and methods for multiphase droplet generation for generating shaped particles and uses thereof
CN110621782A (zh) 监测细胞或来源于其的物质的动态变化的方法及使用其的细胞分类方法
US20250290844A1 (en) Methods of using shaped particles in flow cytometers for assays on b cells and t cells
Dimatteo Democratized Microdroplet Technologies for the Analysis of Single Immune Cell Secretions
De Rutte Probing the Ultimate Limits of Biology: Developing Microparticle Platforms for High-Throughput Single-Cell Assays
CN116615229A (zh) 测定生物细胞的方法
Wang et al. Microfluidic single‐cell technologies for immunotherapeutic target discovery: From design concepts to preclinical applications
WO2025059192A1 (en) Methods of using nanovials to associate secretions and transcriptomes of single cells

Legal Events

Date Code Title Description
PA0105 International application

St.27 status event code: A-0-1-A10-A15-nap-PA0105

P11-X000 Amendment of application requested

St.27 status event code: A-2-2-P10-P11-nap-X000

P13-X000 Application amended

St.27 status event code: A-2-2-P10-P13-nap-X000

PG1501 Laying open of application

St.27 status event code: A-1-1-Q10-Q12-nap-PG1501

A201 Request for examination
E13-X000 Pre-grant limitation requested

St.27 status event code: A-2-3-E10-E13-lim-X000

P11-X000 Amendment of application requested

St.27 status event code: A-2-2-P10-P11-nap-X000

P13-X000 Application amended

St.27 status event code: A-2-2-P10-P13-nap-X000

D13 Search requested

Free format text: ST27 STATUS EVENT CODE: A-1-2-D10-D13-SRH-X000 (AS PROVIDED BY THE NATIONAL OFFICE)

D13-X000 Search requested

St.27 status event code: A-1-2-D10-D13-srh-X000