KR20240012703A - Composition for moisturizing, cell regeneration and improving skin wrinkle including extract of centella asiatica and extract of cynanchum atratum - Google Patents

Abstract

Centella asiatica extract; And a cosmetic composition for improving skin wrinkles containing white rice flower extract is provided.

Description

Composition for moisturizing, cell regeneration and improving skin wrinkles containing centella asiatica extract and white rice flower extract

The present invention relates to a composition for improving skin wrinkles. In detail, it relates to a composition for moisturizing, cell regeneration, and improving skin wrinkles containing centella asiatica extract and white rice flower extract.

Skin is an epithelial tissue that covers and protects the muscles and organs of the body and can be largely divided into the epidermis layer, dermis layer, and subcutaneous fat layer. The skin protects the body's internal organs from the external environment, maintains body temperature, and allows sensation. In addition, the skin contains a sufficient amount of moisture to maintain elasticity and moisture, and plays an important role in maintaining homeostasis, such as regulating body temperature.

When skin elasticity decreases, it appears as wrinkles. There are many reasons why skin elasticity decreases, including degeneration or atrophy of fibrous tissue due to aging and skin dryness due to a decrease in moisture content. Therefore, in order to improve wrinkles caused by the above reasons, it is necessary to ensure that the skin maintains a sufficient amount of moisture. For this purpose, various cosmetic compositions with moisturizing functions are being researched and developed.

In particular, recently, as the elderly population is rapidly increasing, interest in and demand for functional cosmetics such as anti-aging and wrinkle improvement are increasing.

In relation to this, the following patent documents disclose a cosmetic composition containing centella asiatica extract or centella asiatica leaf extract as an active ingredient. Centella asiatica is a perennial plant belonging to the Umbrella Apiaceae family distributed in Korea, Japan, and China. It is reported to be effective in improving skin wrinkles and is used as an active ingredient in various cosmetic compositions.

KR 10-2022-0091242 A KR 10-2022-0087834 A KR 10-2373899 B1 KR 10-2292975 B1 KR 10-2021-0144270 A

The problem to be solved by the present invention is to provide a cosmetic composition that contains centella asiatica extract, white rice flower extract, and seaweed extract as active ingredients, and has excellent effects on skin moisturization, skin cell regeneration, and skin wrinkle improvement. At the same time, the aim is to provide a cosmetic composition with excellent dispersion stability and formulation stability.

The problems of the present invention are not limited to the technical problems mentioned above, and other technical problems not mentioned will be clearly understood by those skilled in the art from the description below.

A composition for moisturizing skin, regenerating skin cells, and/or improving skin wrinkles according to an embodiment of the present invention for solving the above problems includes centella asiatica extract; White rice flower ( cynanchum atratum ) extract; and hololeion maximowiczii extract.

The Centella asiatica extract is a centella asiatica leaf extract and contains 0.01 wt% to 10 wt% of the total weight, the white rice flower extract is a white rice petal extract and contains 0.001 wt% to 5.0 wt% of the total weight, and the seaweed extract is a sea chinensis root extract and contains the total weight. It may contain 0.001wt% to 5.0wt% of the weight.

Centella asiatica is a perennial plant belonging to the Umbrella Apiaceae family distributed in Korea, Japan, and China. It is reported to be effective in improving skin wrinkles and is used as an active ingredient in various cosmetic compositions. Centella asiatica leaf extract can be used as the centella asiatica extract.

White rice flower ( cynanchum atratum ) is a perennial herb belonging to the Gentian family, and the inventor of the present invention completed the present invention by confirming that white rice flower is effective in improving skin wrinkles by using white rice flower as an active ingredient. White rice flower extract can be used as white rice petal extract. The extraction method is not particularly limited, but for example, extraction with an organic solvent such as ethanol, hot water extraction, supercritical extraction, or ultrasonic extraction can be used.

Seaweed ( hololeion maximowiczii ) is a herb belonging to the Campanula Asteraceae family. The inventor of the present invention confirmed that seaweed was effective in improving skin wrinkles by using it as an active ingredient and completed the present invention. As the seaweed extract, seaweed root extract, seaweed grass extract, or seaweed petal extract can be used. Preferably, seaweed root extract is used.

In addition, as a result of various experiments conducted by the present inventor, it was confirmed that homogenization was difficult or floating matter or turbidity occurred depending on the extraction method. In other words, cauliflower root can affect formulation stability depending on the extraction method. This will be explained along with an experimental example.

In some embodiments, the composition may further include oils, such as lavender oil. Lavender oil functions as a skin moisturizer and can inhibit phase separation of the crab extract, which can be somewhat unstable. The crab extract contains some fat content, and an appropriate amount of oil may be added to suppress phase separation with other components. In an exemplary embodiment, the content of lavender oil may be higher than that of white rice flower extract. Additionally, the content of lavender oil may be higher than the content of seaweed extract.

In some further embodiments, the cosmetic composition may further include additional additives to increase formulation stability.

For example, the composition may further include goji berry extract as an active ingredient. In addition, the skin moisturizer may further include one selected from glycerin, butylene glycol, propanediol, adenosine, sodium hyaluronate, and β-glucan, or a combination thereof. Additionally, the composition may use xanthan gum as a viscosity modifier. Additionally, the composition may use trometaine as a pH adjuster. As other solvents, known components such as dipropylene glycol, 1,2-hexanediol, or purified water can be used.

In addition, one or more oil or fat ingredients may be further included. Examples of oil and fat components include ester-based fats and oils, hydrocarbon-based fats and oils, silicone-based fats and oils, fluorine-based fats and oils, animal fats and oils, and vegetable fats and oils. Examples of the ester-based oils include glyceryl tri2-ethylhexanoate, cetyl 2-ethylhexanoate, isopropyl myristate, butyl myristate, isopropyl palmitate, ethyl stearate, octyl palmitate, isocetyl isostearate, and stear. Butyl acid, ethyl linoleate, isopropyl linoleate, ethyl oleate, isocetyl myristate, isostearyl myristate, isostearyl palmitate, octyldodecyl myristate, isocetyl isostearate, diethyl sebacate, adimethylsiloxane Diisopropyl pinate, isoalkyl neopentanoate, tri(caprylic, capric acid)glyceryl, trimethylolpropane tri2-ethylhexanoate, trimethylolpropane triisostearate, pentaelisley tetra2-ethylhexanoate Tol, cetyl caprylate, decyl laurate, hexyl laurate, decyl myristate, myristyl myristate, cetyl myristate, stearyl stearate, decyl oleate, cetyl ricinooleate, isose laurate. Tearyl, isotridecyl myristate, isocetyl palmitate, octyl stearate, isocetyl stearate, isodecyl oleate, octyldodecyl oleate, octyldodecyl linoleate, isopropyl isostearate, 2-ethylhexanoate. Stearyl, 2-ethylhexanoate stearyl, hexyl isostearate, ethylene glycol dioctanoate, ethylene glycol dioleate, propylene glycol dicapric acid, di(capryl, capric acid) propylene glycol, propylene glycol dicaprylate, Neopentyl glycol dicapric acid, neopentyl glycol dioctanate, glyceryl tricaprylate, glyceryl triundecylate, glyceryl triisopalmitate, glyceryl triisostearate, octyldodecyl neopentanoate, isostear octanoate. Reyl, octyl isononanoate, hexyldecyl neodecanoate, octyldodecyl neodecanoate, isocetyl isostearate, isostearyl isostearate, octyldecyl isostearate, polyglycerol oleic acid ester, polyglycerol isostearic acid ester. , Triisocetyl citrate, Triisoalkyl citrate, Triisooctyl citrate, Lauryl lactate, Myristyl lactate, Cetyl lactate, Octyldecyl lactate, Triethyl citrate, Acetyltriethyl citrate, Acetyltributyl citrate, Trioctyl citrate, Mal. Diisostearyl acid, 2-ethylhexyl hydroxystearate, di2-ethylhexyl succinate, diisobutyl adipate, diisopropyl sebacate, dioctyl sebacate, cholesteryl stearate, cholesteryl isostearate. , cholesteryl hydroxystearate, cholesteryl oleate, dihydrocholesteryl oleate, phytsteryl isostearate, phytsteryl oleate, 12-stealoyl isocetyl hydroxystearate, 12-stealoyl Ester systems such as stearyl hydroxystearate and isostearyl 12-stealoylhydroxystearate can be mentioned. Examples of the hydrocarbon oil include squalene, liquid paraffin, alpha-olefin oligomer, isoparaffin, ceresin, paraffin, liquid isoparaffin, polybudene, microcrystalline wax, and petroleum jelly. Examples of the silicone oil include polymethyl silicone, methylphenyl silicone, methylcyclopolysiloxane, octamethylpolysiloxane, decamethylpolysiloxane, dodecamethylcyclosiloxane, dimethylsiloxane/methylcetyloxysiloxane copolymer, dimethylsiloxane/methylstealoxysiloxane copolymer, Alkyl-modified silicone oil, amino-modified silicone oil, etc. can be mentioned. Examples of the fluorine-based fats and oils include perfluoropolyether. The animal or plant oils include avocado oil, almond oil, olive oil, sesame oil, rice bran oil, birdflower oil, soybean oil, corn oil, rapeseed oil, apricot oil, palm kernel oil, palm oil, castor oil, sunflower oil, and grape. Seed oil, cottonseed oil, palm oil, kukuinut oil, wheat germ oil, rice germ oil, shea butter, moonshine colostrum, marker damia nut oil, meadow oil, egg yolk oil, beef tallow, horse oil, mink oil, orange rape oil, jojoba Examples include animal or plant oils such as oil, candelier wax, carnaba wax, liquid lanolin, and hydrogenated castor oil.

In some further embodiments, the composition for moisturizing skin, regenerating skin cells, and/or improving skin wrinkles may further include a fermented extract of Quercus root in addition to the Quercus root extract. Fermented crab cakes may be more beneficial in terms of antioxidant and anti-inflammatory properties compared to crab root extract. Microorganisms used for fermentation include Lactobacillus salivarius, Lactobacillus acidophilus, Lactobacillus brevis, Lactobacillus rhamnosus, and Lactobacillus plantarum. plantarum), Lactobacillus helveticus, Lactobacillus fermentum, Lactobacillus paracasei, Lactobacillus casei, Lactobacillus delbrueckii, Lactobacillus One or more Lactobacillus bacteria among Bacillus reuteri, Lactobacillus buchneri, Lactobacillus gasseri, Lactobacillus johonsonii, and Lactobacillus kefir, or one or more of Lactococcus lactis, Lactococcus plantarum, Lactococcus raffinolactis, or bifidobacterium animals. ), bifidobacterium bifidum, bifidobacterium breve, bifidobacterium infantis, bifidobacterium longum, bifidobacterium capital One or more bifidobacteria may include bifidobacterium pseudolongum, bifidobacterium themophilum, and bifidobacterium adolescentis. Preferably, Lactobacillus casei can be used.

The composition may be formulated and provided as various types of cosmetic compositions. For example, the composition may be formulated in the form of a lotion, liquid, cream, or gel. In this specification, lotion refers to a product made by adding an emulsifier, etc. to homogenize oil-based ingredients and water-based ingredients and make it into a mucus form. In addition, liquid refers to a product made into a liquid state by dissolving it in a solvent, etc. Cream refers to a product made into a semi-solid form by homogenizing oil-based ingredients and water-based ingredients by adding emulsifiers. Gel refers to a semi-solid composition made by infiltrating the composition into large organic molecules.

The method for producing a composition for moisturizing skin, regenerating skin cells, and/or improving skin wrinkles according to an embodiment of the present invention to solve the above problem includes preparing a centella asiatica extract, preparing a white rice flower extract, and preparing a seaweed extract. Including mixing them.

Here, preparing the seaweed extract may include steaming the seaweed root at a temperature of 70°C to 100°C, drying the steamed product with hot air, pulverizing it, and extracting the ground product with an alcohol solvent, filtering, and concentrating the product. .

As a result of various experiments by the present inventor, it was confirmed that homogenization was difficult or floating matter or turbidity occurred depending on the extraction method. In other words, cauliflower root can affect formulation stability depending on the extraction method. This will be explained along with an experimental example.

It is obvious that a step of mixing the various ingredients described above may be further included.

Specific details of other embodiments are included in the detailed description.

According to embodiments of the present invention, it is possible to improve skin moisturization and improve skin wrinkles by including centella asiatica extract, white rice flower extract, and chinensis extract.

Effects according to embodiments of the present invention are not limited to the contents exemplified above, and further various effects are included in the present specification.

The advantages and features of the present invention and methods for achieving them will become clear by referring to the embodiments described in detail below along with the accompanying drawings. However, the present invention is not limited to the embodiments disclosed below and will be implemented in various different forms, and only the embodiments serve to ensure that the disclosure of the present invention is complete, and those skilled in the art It is provided to fully inform the person of the scope of the invention, and the present invention is only defined by the scope of the claims.

Unless otherwise defined, all terms (including technical and scientific terms) used in this specification may be used with meanings that can be commonly understood by those skilled in the art to which the present invention pertains. Additionally, terms defined in commonly used dictionaries are not interpreted ideally or excessively unless clearly specifically defined.

The terminology used herein is for describing embodiments and is not intended to limit the invention. As used herein, 'and/or' includes each and every combination of one or more of the mentioned items. Additionally, the singular form also includes the plural form unless specifically stated in the phrase. As used in the specification, 'comprises' and/or 'comprising' does not exclude the presence or addition of one or more other components in addition to the mentioned components.

The numerical range indicated using the term 'to' used in this specification indicates a numerical range including the values written before and after it as the lower limit and upper limit, respectively. In addition, 'about' or 'approximately' means a value or numerical range within 20% of the value or numerical range stated thereafter.

Hereinafter, the present invention will be described in detail through examples.

<Preparation Example 1: Preparation of Centella asiatica extract>

Centella asiatica leaves were hot-air dried at 55°C for 30 minutes and ground. Then, the dried powder was extracted at room temperature using 70% ethanol. Then, the ethanol extract was obtained by filtration and concentration under reduced pressure.

<Preparation Example 2: Preparation of white rice flower extract>

White rice flower leaves were hot-air dried at 55°C for 30 minutes and ground. Then, the dried powder was extracted at room temperature using 70% ethanol. Then, the ethanol extract was obtained by filtration and concentration under reduced pressure.

<Preparation Example 3-1: Preparation of seaweed extract (1)>

Sesame roots were hot-air dried at 55°C for 2 hours and ground. Then, the dried powder was extracted at room temperature using 70% ethanol. Then, the ethanol extract was obtained by filtration and concentration under reduced pressure.

<Preparation Example 3-2: Preparation of seaweed extract (2)>

Sesame roots were steamed at 90°C for 30 minutes, then dried with hot air at 55°C for 2 hours and ground. Then, the dried powder was extracted at room temperature using 70% ethanol. Then, the ethanol extract was obtained by filtration and concentration under reduced pressure.

<Preparation Example 4: Preparation of fermented crab cake>

Through several experiments, Lactobacillus casei was selected as the fermentation strain. 1 mg of the extract according to Preparation Example 3-2 was mixed with 10 ml of purified water, inoculated with Lactobacillus casei, and cultured in an incubator at 55°C for 36 hours. Then, the culture was centrifuged to obtain the supernatant and concentrated.

<Experimental Example 1>

MTT assay (Sigma-Aldrich) was performed to confirm the cytotoxicity of the products obtained in Preparation Example 1, Preparation Example 2, Preparation Example 3-1, Preparation Example 3-2, and Preparation Example 3-3. Human Keratinocyte cells (HaCaT) were inoculated into a 96-well plate at a concentration of 1.0 × 10 ⁵ cell/ml and cultured in a 5% CO ₂ humidified incubator at 37°C for 18 hours.

After incubation, the cells were washed with PBS buffer, each composition was diluted according to concentration, and the cells were further cultured for 24 hours. After treating with 0.5mg/ml MTT reagent and reacting for 1 hour, the formed formazan was dissolved in 200㎕ of DMSO (dimethyl sulfoxide) and the absorbance was measured at 590nm using a spectrophotometer.

And the results are shown in Table 1 below.

Cell viability
(% of control) Manufacturing Example 1 10ppm 101.24±2.57 100 ppm 102.68±4.69 Production example 2 10ppm 103.45±3.54 100ppm 99.57±3.99 Manufacturing Example 3-1 10 ppm 98.45±4.78 100 ppm 100.25±6.91 Manufacturing Example 3-2 10ppm 99.25±7.32 100ppm 97.57±5.36 Production example 4 10ppm 99.36±4.12 100ppm 101.35±6.22

<Preparation Example 5: Preparation of mixed composition>

A composition was prepared as shown in Table 2 below.

Example One 2 3 4 5 Manufacturing Example 1 0.5 0.5 0.5 0.5 0.5 Production example 2 0.5 0.5 0.5 0.5 Manufacturing Example 3-1 1.0 Manufacturing Example 3-2 1.0 0.5 Production example 4 0.5 chrysanthemum extract 1.0 glycerin 9.5 Butylene glycol 6.5 Propanediol 1.0 Adenosine 0.1 Sodium Hyaluronate 0.1 glucan 0.1 xanthan gum 0.5 Trometaine 0.15 Dipropylene glycol 10 Purified water to 100

<Experimental Example 2>

An experiment was conducted to confirm the effect of the above examples on improving skin wrinkles. Human dermal fibroblast (HDF) was treated with the composition according to the examples at 100 ppm. Then, the produced procollagen was quantified and the results compared to the control group (untreated) are shown in Table 3 below.

Relative production
(% of control) Example 1 127.51±5.31 Example 2 144.25±5.69 Example 3 150.14±3.42 Example 4 155.37±6.17 Example 5 125.19±4.99

Referring to Table 3, it can be seen that Examples 2 to 5 exhibit significantly higher procollagen production characteristics compared to Example 1. It can be seen that Example 5, used as a comparative example, does not show any significant difference from Example 1.

<Experimental Example 3>

An experiment was conducted to confirm the skin moisturizing effect of the above examples. HaCaT cells were cultured in the same manner as Experimental Example 1. Then, the composition according to each example was treated at a concentration of 100 μM and further cultured for 24 hours. The culture was placed in a tube, treated with 0.2 ml of chloroform, and centrifuged at 13,000 rpm for 10 minutes after 10 minutes. Then, the supernatant was put back into a new tube, treated with 0.3 ml of isopropyl alcohol, and centrifuged at 13,000 rpm for 10 minutes after 10 minutes. Then, the supernatant was put back into a new tube, treated with 0.3 ml of 75% ethanol, and centrifuged at 13,000 rpm for 5 minutes after 10 minutes. After removing the supernatant, it was dried, RNA was quantified, and cDNA was synthesized. And the expression level of target proteins (hyaluronic acid synthase-3 (HAS-3), aquaporin-3 (AQP-3)) was evaluated through real-time polymerase chain reaction. The results compared to the control group are shown in Table 4 below. The primer sequences used are listed in Table 5 below.

Relative mRNA expression
(% of control) Example 1 114.39±4.11 Example 2 137.21±5.78 Example 3 139.13±6.54 Example 4 146.82±6.77 Example 5 109.49±6.58

primer direction order sequence number HAS-3-F forward 5’-CTTAAGGGTTGCTTGCTTGC-3’ One HAS-3-R reverse 5’-GTTCGTGGGAGATGAAGGAA-3’ 2 AQP-3-F forward 5'-AGACAGCCCCTTCAGGATTT-3' 3 AQP-3-R reverse 5'-TCCCTTGCCCTGAATATCTG-3' 4

Referring to Table 4, it can be seen that Examples 2 to 5 show significantly higher expression compared to Example 1.

<Production Example 6>

A composition was prepared as shown in Table 6 below.

Example 6 7 Manufacturing Example 1 0.5 0.5 Production example 2 0.5 0.5 Manufacturing Example 3-2 1.0 0.5 Production example 4 0.5 lavender oil 1.25 1.25 glycerin 9.0 Butylene glycol 6.0 Propanediol 1.0 Adenosine 0.1 Sodium Hyaluronate 0.1 glucan 0.1 xanthan gum 0.5 Trometaine 0.15 Dipropylene glycol 10 Purified water to 100

<Experimental Example 4>

Formulation stability evaluation was conducted using the compositions according to Examples 1 to 4, Example 6, and Example 7. The composition was stored at 40°C and the change in turbidity over 9 weeks was confirmed, and the results are shown in Table 7 below. In Table 7 below, △ means that a slight change was observed compared to the initial time, and × means that a complete change in an unfavorable direction was observed.

Example One 2 3 4 6 7 1 week - - - - - - 3 weeks - △ - - - - 5 weeks - △ △ △ - - 7 weeks - × △ △ - - 9 weeks △ × × △ △ △

Referring to Table 7 above, it can be seen that Example 1 shows very stable formulation properties even when stored at high temperature for a long time. On the other hand, it can be seen that Examples 2 to 4 are somewhat unstable over time. Comparing Example 2 and Example 3, it can be seen that compared to Example 2, which used a simple hot air-dried extract of crab root, Example 3, which used an extract of steamed crab root and then hot-air dried it, showed relatively more stable properties. Furthermore, it can be confirmed that Examples 6 and 7, in which lavender oil was mixed in a sufficient amount, had stability at the level of Example 1.

In the above, the description has been made focusing on the embodiments of the present invention, but this is only an example and does not limit the present invention, and those skilled in the art will understand the present invention without departing from the essential characteristics of the embodiments of the present invention. It will be apparent that various modifications and applications not illustrated above are possible.

Therefore, the scope of the present invention should be understood to include changes, equivalents, or substitutes of the technical ideas exemplified above. For example, each component specifically shown in the embodiments of the present invention can be modified and implemented. And these variations and differences in application should be construed as being included in the scope of the present invention as defined in the appended claims.

Claims (7)

Centella asiatica extract;
White rice flower ( cynanchum atratum ) extract; and
A composition for improving wrinkles, comprising a hololeion maximowiczii extract. According to paragraph 1,
It further comprises lavender oil with a high content of each of the white rice flower extract and the crab extract,
The Centella asiatica extract is a Centella asiatica leaf extract and contains 0.01 wt% to 10 wt% of the total weight,
The white rice flower extract is a white rice petal extract and contains 0.001 wt% to 5.0 wt% of the total weight,
The seaweed extract is a seaweed root extract and is included in an amount of 0.001 wt% to 5.0 wt% of the total weight of the composition for improving wrinkles. According to paragraph 2,
glycerin; butylene glycol; Propanediol; adenosine; sodium hyaluronate; β-glucan; xanthan gum; trometaine; Goji berry extract; dipropylene glycol; 1,2-hexanediol; and purified water. According to paragraph 1,
A composition further comprising a fermented extract of Lactobacillus casei. According to paragraph 1,
The composition is formulated in the form of a lotion, liquid, cream, or gel. Prepare Centella asiatica extract,
Prepare white rice flower extract,
Including preparing seaweed extract and mixing them,
Preparing the crab extract is,
The roots of the crab root are steamed at a temperature of 70°C to 100°C,
The steamed product is dried with hot air and then pulverized.
A method for producing a composition for improving wrinkles, comprising extracting, filtering, and concentrating the pulverized material with an alcohol solvent. According to clause 6,
A method comprising further mixing the fermented crab extract with Lactobacillus casei.