KR20240011489A - Pharmaceutical composition for preventing or treating diabetes by DPP4 hyperexpression comprising Glycyrrhiza uralensis extracts as an active ingredient - Google Patents
Pharmaceutical composition for preventing or treating diabetes by DPP4 hyperexpression comprising Glycyrrhiza uralensis extracts as an active ingredient Download PDFInfo
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- KR20240011489A KR20240011489A KR1020220088983A KR20220088983A KR20240011489A KR 20240011489 A KR20240011489 A KR 20240011489A KR 1020220088983 A KR1020220088983 A KR 1020220088983A KR 20220088983 A KR20220088983 A KR 20220088983A KR 20240011489 A KR20240011489 A KR 20240011489A
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- dpp4
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Classifications
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
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- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
- A61K36/484—Glycyrrhiza (licorice)
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- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
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- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
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- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
- A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
- A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2200/00—Function of food ingredients
- A23V2200/30—Foods, ingredients or supplements having a functional effect on health
- A23V2200/328—Foods, ingredients or supplements having a functional effect on health having effect on glycaemic control and diabetes
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Abstract
본 발명은 감초(Glycyrrhiza uralensis) 추출물을 유효성분으로 포함하는 DPP4(dipeptidyl peptidase 4) 과발현 당뇨병 예방 또는 치료용 약학 조성물에 관한 것으로, 감초 추출물이 DPP4 억제 효과를 나타내는 것을 확인함으로써, DPP4 과발현 당뇨병 예방, 치료 또는 개선용 조성물로서 유용하게 활용될 수 있다.The present invention is licorice ( Glycyrrhiza uralensis ) extract as an active ingredient for the prevention or treatment of diabetes overexpressing DPP4 (dipeptidyl peptidase 4). By confirming that the licorice extract exhibits a DPP4 inhibitory effect, the composition was used to prevent, treat or improve diabetes overexpressing DPP4. It can be useful.
Description
본 발명은 감초(Glycyrrhiza uralensis) 추출물을 유효성분으로 포함하는 DPP4(dipeptidyl peptidase 4) 과발현 당뇨병 예방 또는 치료용 약학 조성물에 관한 것이다.The present invention is licorice ( Glycyrrhiza uralensis ) extract as an active ingredient and relates to a pharmaceutical composition for preventing or treating diabetes overexpressing DPP4 (dipeptidyl peptidase 4).
당뇨병은 세계적으로 사람들에게 미치는 영향이 빠르게 증가하는 만성 대사 질환이다. 당뇨병은 2019년 전 세계 4억 6300만 명에게 영향을 미쳤고, 2030년에는 5억 7800만 명, 2045년에는 7억 명으로 증가할 것으로 예상된다. 게다가, 당뇨병은 전 세계 사망자의 약 10%를 차지하는 것으로 추정된다. 그 중 제2형 당뇨병(T2DM)이 가장 흔하며, 환자의 약 90%를 차지하고 있다. 당뇨병은 말초 조직에서 췌장 인슐린 생산 및/또는 인슐린 저항성이 불충분한 것이 특징이고, 당뇨병과 관련된 만성 고혈당은 눈, 신장, 신경, 심장 등 중요한 장기의 장기 손상과 관련이 있다.Diabetes is a chronic metabolic disease with a rapidly increasing impact on people worldwide. Diabetes affected 463 million people worldwide in 2019, and is expected to increase to 578 million in 2030 and 700 million in 2045. Additionally, diabetes is estimated to account for approximately 10% of deaths worldwide. Among them, type 2 diabetes mellitus (T2DM) is the most common, accounting for approximately 90% of patients. Diabetes is characterized by insufficient pancreatic insulin production and/or insulin resistance in peripheral tissues, and chronic hyperglycemia associated with diabetes is associated with long-term damage to vital organs such as the eyes, kidneys, nerves, and heart.
“인크레틴(incretin)”은 GLP-1(glucagon-like peptide-1) 및 GIP(glucose-dependent insulinotropic polypeptide)를 포함하는 호르몬 그룹을 의미하고, 음식을 섭취 시, 인슐린 생산을 자극하기 위해 췌장 β세포에 작용하여 내장에서 생산된다. DPP4(dipeptidyl peptidase 4)는 1분 이내에 순환 활성 GLP-1 및 GIP를 감소시키고, DPP4 억제제는 DPP4를 억제하고 활성 GLP-1 및 GIP 수준을 향상시켜 이들의 작용을 촉진하는 약물이다. 따라서, DPP4 억제제는 제2형 당뇨병의 치료를 위한 새로운 치료법으로 등장했다. 그러나 현재 DPP4 억제제는 실제 적용을 제한하는 몇 가지 부작용을 가지고 있다. 따라서, 약초 및 식물과 같은 천연자원으로부터 파생된 새로운 DPP4 억제제에 대한 치료적 필요성이 대두되고 있다.“Incretin” refers to a group of hormones that include glucagon-like peptide-1 (GLP-1) and glucose-dependent insulinotropic polypeptide (GIP), which, upon ingestion, stimulate the production of insulin by the pancreas. It acts on cells and is produced in the intestines. DPP4 (dipeptidyl peptidase 4) reduces circulating active GLP-1 and GIP within 1 minute, and DPP4 inhibitors are drugs that promote their action by inhibiting DPP4 and enhancing active GLP-1 and GIP levels. Therefore, DPP4 inhibitors have emerged as a new therapy for the treatment of type 2 diabetes. However, current DPP4 inhibitors have several side effects that limit their practical application. Therefore, there is an emerging therapeutic need for new DPP4 inhibitors derived from natural resources such as herbs and plants.
감초(Glycyrrhiza uralensis)는 콩과(Leguminosae)에 속하는 약용식물로 잘 알려져 있다. 현재까지 감초로부터 다양한 생물학적 활성 성분이 확인되었고, 주요 구성 요소로는 트리테르펜 사포닌(triterpene saponin), 플라보노이드(flavonoid) 등이 있다. 감초 및 감초의 활성 성분의 다양한 약리작용들이 보고되고 있으나, 감초 및 감초의 활성 성분의 DPP4 억제를 통한 항당뇨 효과에 대해서는 아직까지 보고된 바 없다.Licorice ( Glycyrrhiza) uralensis ) is well known as a medicinal plant belonging to the leguminosae family. To date, various biologically active ingredients have been identified from licorice, and the main components include triterpene saponin and flavonoid. Various pharmacological effects of licorice and its active ingredients have been reported, but the anti-diabetic effect of licorice and its active ingredients through DPP4 inhibition has not yet been reported.
본 발명의 목적은 감초(Glycyrrhiza uralensis) 추출물을 유효성분으로 포함하는 DPP4(dipeptidyl peptidase 4) 과발현 당뇨병 예방 또는 치료용 약학 조성물을 제공하는 것이다.The object of the present invention is licorice ( Glycyrrhiza uralensis ) extract as an active ingredient to provide a pharmaceutical composition for preventing or treating DPP4 (dipeptidyl peptidase 4) overexpression diabetes.
본 발명의 다른 목적은 감초(Glycyrrhiza uralensis) 추출물을 유효성분으로 포함하는 DPP4(dipeptidyl peptidase 4) 과발현 당뇨병 예방 또는 개선용 건강기능식품 조성물을 제공하는 것이다.Another object of the present invention is licorice ( Glycyrrhiza uralensis ) extract as an active ingredient to provide a health functional food composition for preventing or improving DPP4 (dipeptidyl peptidase 4) overexpression diabetes.
상기 목적을 달성하기 위해, 본 발명은 감초(Glycyrrhiza uralensis) 추출물을 유효성분으로 포함하는 DPP4(dipeptidyl peptidase 4) 과발현 당뇨병 예방 또는 치료용 약학 조성물을 제공한다. In order to achieve the above object, the present invention is licorice ( Glycyrrhiza Provided is a pharmaceutical composition for preventing or treating diabetes overexpressing DPP4 (dipeptidyl peptidase 4), which contains an extract of DPP4 (dipeptidyl peptidase 4) as an active ingredient.
또한, 본 발명은 감초(Glycyrrhiza uralensis) 추출물을 유효성분으로 포함하는 DPP4(dipeptidyl peptidase 4) 과발현 당뇨병 예방 또는 개선용 건강기능식품 조성물을 제공한다.In addition, the present invention is licorice ( Glycyrrhiza Provides a health functional food composition for preventing or improving diabetes overexpressing DPP4 (dipeptidyl peptidase 4), which contains an extract of uralensis ) as an active ingredient.
본 발명에 따르면, 감초(Glycyrrhiza uralensis) 추출물이 DPP4(dipeptidyl peptidase 4) 억제 효과를 나타내는 것을 확인함으로써, DPP4 과발현 당뇨병 예방, 치료 또는 개선용 조성물로서 유용하게 활용될 수 있다.According to the present invention, licorice ( Glycyrrhiza uralensis ) extract has an inhibitory effect on DPP4 (dipeptidyl peptidase 4), so it can be usefully used as a composition for preventing, treating or improving DPP4 overexpression diabetes.
도 1은 감초(Glycyrrhiza uralensis) 열수 추출물(crude water extract; CWE)의 농도에 따른 DPP4(dipeptidyl peptidase 4)의 상대적 억제율을 분석한 결과이다. Figure 1 shows licorice ( Glycyrrhiza uralensis ) This is the result of analyzing the relative inhibition rate of DPP4 (dipeptidyl peptidase 4) according to the concentration of crude water extract (CWE).
이하, 본 발명을 보다 상세하게 설명한다.Hereinafter, the present invention will be described in more detail.
본 발명은 감초(Glycyrrhiza uralensis) 추출물을 유효성분으로 포함하는 DPP4(dipeptidyl peptidase 4) 과발현 당뇨병 예방 또는 치료용 약학 조성물을 제공한다. The present invention is licorice ( Glycyrrhiza Provided is a pharmaceutical composition for preventing or treating diabetes overexpressing DPP4 (dipeptidyl peptidase 4), which contains an extract of DPP4 (dipeptidyl peptidase 4) as an active ingredient.
상기 추출물은 물, (C1-C4) 알코올 및 이의 혼합물로 이루어진 군에서 선택된 용매로 추출할 수 있으나, 이에 한정되는 것은 아니다.The extract may be extracted with a solvent selected from the group consisting of water, (C1-C4) alcohol, and mixtures thereof, but is not limited thereto.
또한, 상기 추출물은 DPP4 활성을 억제할 수 있다.Additionally, the extract can inhibit DPP4 activity.
본 발명의 약학 조성물은 당해 발명이 속하는 기술 분야에서 통상의 지식을 가진 자가 용이하게 실시할 수 있는 방법에 따라, 약제학적으로 허용되는 담체를 이용하여 제제화함으로써 단위 용량 형태로 제조되거나 또는 다용량 용기 내에 내입시켜 제조될 수 있다.The pharmaceutical composition of the present invention is prepared in unit dose form or in a multi-dose container by formulating it using a pharmaceutically acceptable carrier according to a method that can be easily performed by a person skilled in the art. It can be manufactured by internalizing it.
상기 약제학적으로 허용되는 담체는 제제시에 통상적으로 이용되는 것으로서, 락토스, 덱스트로스, 수크로스, 솔비톨, 만니톨, 전분, 아카시아 고무, 인산 칼슘, 알기네이트, 젤라틴, 규산 칼슘, 미세결정성 셀룰로스, 폴리비닐피롤리돈, 셀룰로스, 물, 시럽, 메틸 셀룰로스, 메틸 히드록시벤조에이트, 프로필히드록시벤조에이트, 활석, 스테아르산 마그네슘, 미네랄 오일 등을 포함하나, 이에 한정되는 것은 아니다. 본 발명의 약학 조성물은 상기 성분들 이외에 윤활제, 습윤제, 감미제, 향미제, 유화제, 현탁제, 보존제 등을 추가로 포함할 수 있다.The pharmaceutically acceptable carriers are those commonly used in preparation, and include lactose, dextrose, sucrose, sorbitol, mannitol, starch, gum acacia, calcium phosphate, alginate, gelatin, calcium silicate, microcrystalline cellulose, Includes, but is not limited to, polyvinylpyrrolidone, cellulose, water, syrup, methyl cellulose, methyl hydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate, mineral oil, etc. In addition to the above components, the pharmaceutical composition of the present invention may further include lubricants, wetting agents, sweeteners, flavoring agents, emulsifiers, suspending agents, preservatives, etc.
본 발명에 있어서, 상기 약학 조성물에 포함되는 첨가제의 함량은 특별히 한정되는 것은 아니며 통상의 제제화에 사용되는 함량 범위 내에서 적절하게 조절될 수 있다.In the present invention, the content of additives included in the pharmaceutical composition is not particularly limited and can be appropriately adjusted within the content range used in conventional formulations.
상기 약학 조성물은 수용액, 현탁액, 유탁액 등과 같은 주사용 제형, 환약, 캡슐, 과립, 정제, 크림, 젤, 패취, 분무제, 연고제, 경고제, 로션제, 리니멘트제, 파스타제 및 카타플라스마제로 이루어진 군에서 선택된 하나 이상의 피부 외용제 형태로 제형화될 수 있으나, 이에 한정되는 것은 아니다.The pharmaceutical compositions include injectable formulations such as aqueous solutions, suspensions, and emulsions, pills, capsules, granules, tablets, creams, gels, patches, sprays, ointments, warning agents, lotions, liniment agents, paste agents, and cataplasmase agents. It may be formulated in the form of one or more external skin preparations selected from the group consisting of, but is not limited to this.
본 발명의 약학 조성물은 제형화를 위해 추가로 있는 약학적으로 허용 가능한 담체 및 희석제를 포함할 수 있다. 상기 약학적으로 허용 가능한 담체 및 희석제는 전분, 당 및 만니톨과 같은 부형제, 칼슘 포스페이트 등과 같은 충전제 및 증량제, 카르복시메틸셀룰로오스, 히드록시프로필셀룰로오스 등과 같은 셀룰로오스 유도체, 젤라틴, 알긴산염, 폴리비닐 피롤리돈 등과 같은 결합제, 활석, 스테아린산 칼슘, 수소화 피마자유 및 폴리에틸렌글리콜과 같은 윤활제, 포비돈 및 크로스포비돈과 같은 붕해제, 폴리소르베이트, 세틸알코올, 글리세롤 등과 같은 계면활성제를 포함하나, 이에 한정되지 않는다. 상기 약학적으로 허용 가능한 담체 및 희석제는 대상체에게 생물학적 및 생리학적으로 친화적인 것일 수 있다. 희석제의 예로는 염수, 수용성 완충액, 용매 및/또는 분산제(dispersion media)를 들 수 있으나, 이에 제한되는 것은 아니다.The pharmaceutical composition of the present invention may additionally contain pharmaceutically acceptable carriers and diluents for formulation. The pharmaceutically acceptable carriers and diluents include excipients such as starch, sugar and mannitol, fillers and extenders such as calcium phosphate, cellulose derivatives such as carboxymethylcellulose, hydroxypropylcellulose, gelatin, alginate, polyvinyl pyrrolidone. It includes, but is not limited to, binders such as talc, calcium stearate, lubricants such as hydrogenated castor oil and polyethylene glycol, disintegrants such as povidone and crospovidone, and surfactants such as polysorbate, cetyl alcohol, glycerol, etc. The pharmaceutically acceptable carrier and diluent may be biologically and physiologically friendly to the subject. Examples of diluents include, but are not limited to, saline, aqueous buffers, solvents, and/or dispersion media.
본 발명의 약학 조성물은 목적하는 방법에 따라 경구 투여하거나 비경구 투여(예를 들어, 정맥 내, 피하, 복강 내 또는 국소에 적용)할 수 있다. 경구 투여일 경우, 정제, 트로키제(troches), 로젠지(lozenge), 수용성 현탁액, 유성 현탁액, 조제 분말, 과립, 에멀젼, 하드 캡슐, 소프트 캡슐, 시럽, 엘릭시르제 등으로 제형화될 수 있다. 비경구 투여일 경우, 주사액, 좌제, 호흡기 흡입용 분말, 스프레이용 에어로졸제, 연고, 도포용 파우더, 오일, 크림 등으로 제형화 될 수 있다.The pharmaceutical composition of the present invention can be administered orally or parenterally (for example, intravenously, subcutaneously, intraperitoneally, or topically) depending on the desired method. For oral administration, it can be formulated as tablets, troches, lozenges, aqueous suspensions, oily suspensions, powders, granules, emulsions, hard capsules, soft capsules, syrups, elixirs, etc. In the case of parenteral administration, it can be formulated as an injection, suppository, powder for respiratory inhalation, aerosol for spray, ointment, powder for application, oil, cream, etc.
본 발명의 약학 조성물의 투여량은 환자의 상태, 체중, 연령, 성별, 건강상태, 식이 체질 특이성, 제제의 성질, 질병의 정도, 조성물의 투여시간, 투여방법, 투여기간 또는 간격, 배설율 및 약물 형태에 따라 그 범위가 다양할 수 있으며, 이 분야 통상의 기술자에 의해 적절하게 선택될 수 있다. 예컨대, 약 0.1 내지 10,000mg/kg의 범위일 수 있으나 이제 제한되지 않으며, 하루 일회 내지 수회에 나누어 투여될 수 있다.The dosage of the pharmaceutical composition of the present invention is determined by the patient's condition, weight, age, gender, health, dietary constitution specificity, nature of the preparation, degree of disease, administration time of the composition, administration method, administration period or interval, excretion rate, and The range may vary depending on the drug form and can be appropriately selected by a person skilled in the art. For example, it may range from about 0.1 to 10,000 mg/kg, but is not limited and may be administered once to several times a day.
상기 약학 조성물은 목적하는 방법에 따라 경구 투여되거나 비경구 투여(예를 들면, 정맥 내, 피하 내, 복강 내 또는 국소에 적용)될 수 있다. 본 발명의 약학 조성물의 약학적 유효량 및 유효 투여량은 약학 조성물의 제제화 방법, 투여 방식, 투여 시간, 투여 경로 등에 의해 다양해질 수 있으며, 당해 기술 분야에서 통상의 지식을 가진 자는 목적하는 치료에 효과적인 투여량을 용이하게 결정하고 처방할 수 있다. 본 발명의 약학 조성물의 투여는 하루에 1회 투여될 수 있고, 수회에 나누어 투여될 수도 있다.The pharmaceutical composition may be administered orally or parenterally (eg, intravenously, subcutaneously, intraperitoneally, or topically applied) depending on the desired method. The pharmaceutically effective amount and effective dosage of the pharmaceutical composition of the present invention may vary depending on the formulation method, administration method, administration time, administration route, etc. of the pharmaceutical composition, and those skilled in the art will know that it is effective for the desired treatment. Dosage can be easily determined and prescribed. The pharmaceutical composition of the present invention may be administered once a day, or may be administered in several divided doses.
또한, 본 발명은 감초(Glycyrrhiza uralensis) 추출물을 유효성분으로 포함하는 DPP4(dipeptidyl peptidase 4) 과발현 당뇨병 예방 또는 개선용 건강기능식품 조성물을 제공한다.In addition, the present invention is licorice ( Glycyrrhiza Provides a health functional food composition for preventing or improving diabetes overexpressing DPP4 (dipeptidyl peptidase 4), which contains an extract of uralensis ) as an active ingredient.
본 발명은 통상적으로 이용되는 식품으로써 일반적으로 사용될 수 있다.The present invention can be generally used with commonly used foods.
본 발명의 식품 조성물은 건강기능식품으로서 사용될 수 있다. 상기 “건강기능식품”이라 함은 건강기능 식품에 관한 법률에 따른 인체에 유용한 기능성을 가진 원료나 성분을 사용하여 제조 및 가공한 식품을 의미하며, “기능성”이라 함은 인체의 구조 및 기능에 대하여 영양소를 조절하거나 생리학적 작용 등과 같은 보건용도에 유용한 효과를 얻을 목적으로 섭취하는 것을 의미한다.The food composition of the present invention can be used as a health functional food. The term “health functional food” refers to food manufactured and processed using raw materials or ingredients with functionality useful to the human body in accordance with the Health Functional Food Act, and “functionality” refers to food that is related to the structure and function of the human body. It means ingestion for the purpose of controlling nutrients or obtaining useful health effects such as physiological effects.
상기 건강기능식품 조성물은 통상의 식품 첨가물을 포함할 수 있으며, 상기 “식품 첨가물”로서의 적합 여부는 다른 규정이 없는 한, 식품의약품안전처에 승인된 식품 첨가물 공전의 총칙 및 일반시험법 등에 따라 해당 품목에 관한 규격 및 기준에 의하여 판정한다.The health functional food composition may contain common food additives, and its suitability as a “food additive” is determined in accordance with the general provisions and general test methods of the food additive code approved by the Ministry of Food and Drug Safety, unless otherwise specified. The decision is made based on the specifications and standards for the item.
상기 “식품 첨가물 공전”에 수재된 품목으로는 예를 들어, 케톤류, 글리신, 구연산칼륨, 니코틴산, 계피산 등의 화학적 합성물, 감색소, 감초추출물, 결정셀룰로오스, 고량색소, 구아검 등의 천연첨가물, L-글루타민산나트륨 제제, 면류첨가알칼리제, 보존료제제, 타르색소제제 등의 혼합제제류들을 들 수 있다.Items listed in the “Food Additives Code” include, for example, chemical compounds such as ketones, glycine, potassium citrate, nicotinic acid, and cinnamic acid; natural additives such as subchromic pigment, licorice extract, crystalline cellulose, high-liquid pigment, and guar gum; Examples include mixed preparations such as sodium L-glutamate preparations, noodle additive alkaline preparations, preservative preparations, and tar coloring preparations.
본 발명의 식품 조성물은 정제, 캡슐, 분말, 과립, 액상, 환 등의 형태로 제조 및 가공할 수 있다. 예를 들어, 캡슐 형태의 건강기능 식품 중 경질 캡슐제는 통상의 경질 캡슐에 본 발명에 따른 조성물을 부형제 등의 첨가제와 혼합 및 충진 하여 제조할 수 있으며, 연질 캡슐제는 본 발명에 따른 조성물을 부형제 등의 첨가제와 혼합하고 젤라틴 등 캡슐기제에 충진하여 제조할 수 있다. 상기 연질 캡슐제 는 필요에 따라 글리세린 또는 소르비톨 등의 가소제, 착색제, 보존제 등을 함유할 수 있다.The food composition of the present invention can be manufactured and processed in the form of tablets, capsules, powders, granules, liquids, pills, etc. For example, among health functional foods in the form of capsules, hard capsules can be manufactured by mixing and filling the composition according to the present invention with additives such as excipients in a regular hard capsule, and soft capsules can be manufactured by mixing and filling the composition according to the present invention. It can be manufactured by mixing with additives such as excipients and filling it with a capsule base such as gelatin. The soft capsule may contain plasticizers such as glycerin or sorbitol, colorants, preservatives, etc., if necessary.
상기 부형제, 결합제, 붕해제, 활택제, 교미제, 착향제 등에 대한 용어 정의는 당업계에 공지된 문헌에 기재된 것으로 그 기능 등이 동일 내지 유사한 것들을 포함한다. 상기 식품의 종류에는 특별한 제한이 없으며, 통상적인 의미에서의 건강 기능식품을 모두 포함한다.Definitions of terms such as excipients, binders, disintegrants, lubricants, coagulants, flavoring agents, etc. are described in literature known in the art and include those with the same or similar functions. There is no particular limitation on the type of food, and it includes all health functional foods in the conventional sense.
본 발명에서 용어 “예방”은 본 발명에 따른 조성물의 투여로 DPP4 과발현 당뇨병 질환을 억제 또는 지연시키는 모든 행위를 말한다. In the present invention, the term “prevention” refers to all actions that inhibit or delay DPP4 overexpression diabetic disease by administering the composition according to the present invention.
본 발명에서 용어 “치료”는 본 발명에 따른 조성물의 투여로 DPP4 과발현 당뇨병 질환의 증세를 호전시키거나 이롭게 변경하는 모든 행위를 말한다. In the present invention, the term “treatment” refers to any action that improves or beneficially changes the symptoms of DPP4 overexpression diabetic disease by administering the composition according to the present invention.
본 발명에서 용어 “개선”은 본 발명에 따른 조성물의 투여로 DPP4 과발현 당뇨병 질환의 나쁜 상태를 좋게 하는 모든 행위를 말한다.In the present invention, the term “improvement” refers to any action that improves the bad condition of DPP4 overexpression diabetic disease by administering the composition according to the present invention.
이하, 본 발명의 이해를 돕기 위하여 실시예를 들어 상세하게 설명하기로 한다. 다만 하기의 실시예는 본 발명의 내용을 예시하는 것일 뿐 본 발명의 범위가 하기 실시예에 한정되는 것은 아니다. 본 발명의 실시예는 당업계에서 평균적인 지식을 가진 자에게 본 발명을 보다 완전하게 설명하기 위해 제공되는 것이다.Hereinafter, the present invention will be described in detail through examples to aid understanding. However, the following examples only illustrate the content of the present invention and the scope of the present invention is not limited to the following examples. Examples of the present invention are provided to more completely explain the present invention to those skilled in the art.
[실시예 1] DPP4 효소 활성 분석([Example 1] DPP4 enzyme activity analysis ( in vitroin vitro ))
감초(Glycyrrhiza uralensis) 열수 추출물(crude water extract; CWE)의 Dipeptidyl peptidase 4(이하 DPP4라 함) 억제 활성을 확인하기 위해, DPP4 억제제 스크리닝 키트(Sigma Aldrich, St. Louis, MO, USA)를 사용하였다. 마이크로웰 플레이트 리더를 사용하여 웰(well) 내 존재하는 효소 활성에 비례하는 형광 생성물(fluorescent product, μex = 360nm, μem = 460nm)의 분열(cleavage)을 측정하였다. 형광 방출은 30분 동안 검은 웰 바닥의 96 웰 플레이트에서 시간대별 활성을 (Kinetic mode) 측정하였고, 상대적 억제 비율을 계산하기 위해, 하기 수학식 1을 사용하였다. ΔF/ΔT는 주어진 시간 간격 동안의 형광의 변화를 나타내고, 그래프는 구글 시트(Google Sheets)로 작성하였다.Licorice ( Glycyrrhiza) uralensis crude water extract (CWE), a DPP4 inhibitor screening kit (Sigma Aldrich, St. Louis, MO, USA) was used to confirm the Dipeptidyl peptidase 4 (hereinafter referred to as DPP4) inhibitory activity. Cleavage of the fluorescent product (μex = 360nm, μem = 460nm), which was proportional to the enzyme activity present in the well, was measured using a microwell plate reader. Fluorescence emission was measured over time (kinetic mode) in a 96-well plate with a black well bottom for 30 minutes, and the following equation 1 was used to calculate the relative inhibition ratio. ΔF/ΔT represents the change in fluorescence during a given time interval, and the graph was prepared using Google Sheets.
[수학식 1][Equation 1]
그 결과, 도 1에 나타난 바와 같이, 감초 열수 추출물이 다양한 농도에서 DPP4 억제 효과를 나타냈고, 구체적으로, 800μg/ml 농도에서 감초 열수 추출물이 DPP4의 활성을 21.6%까지 억제하는 것을 확인하였다.As a result, as shown in Figure 1, the licorice hot water extract showed an inhibitory effect on DPP4 at various concentrations. Specifically, it was confirmed that the licorice hot water extract inhibited the activity of DPP4 by 21.6% at a concentration of 800 μg/ml.
이상으로 본 발명 내용의 특정한 부분을 상세히 기술하였는바, 당업계의 통상의 지식을 가진 자에게 있어서, 이러한 구체적 기술은 단지 바람직한 실시양태일 뿐이며, 이에 의해 본 발명의 범위가 제한되는 것이 아닌 점은 명백하다. 즉, 본 발명의 실질적인 범위는 첨부된 청구항들과 그것들의 등가물에 의하여 정의된다.As the specific parts of the present invention have been described in detail above, it is clear to those skilled in the art that these specific techniques are merely preferred embodiments and do not limit the scope of the present invention. do. That is, the practical scope of the present invention is defined by the appended claims and their equivalents.
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