KR20230171329A - Cosmetic composition for anti-inflammation, anti-oxidation and skin protection against UV comprising biorenovation extract of black tea as effective component - Google Patents

Abstract

The present invention relates to a cosmetic composition for anti-inflammatory, antioxidant and skin protection against ultraviolet rays containing black tea bioconversion extract as an active ingredient. Specifically, the black tea bioconversion extract prepared through the bioconversion method is non-cytotoxic, and the black tea bioconversion extract is non-cytotoxic. Compared to extracts, it has superior skin protection and antioxidant activity against ultraviolet rays, and has the effect of significantly suppressing the production of NO (nitric oxide), PGE ₂ (prostaglandin E ₂ ), and pro-inflammatory cytokines induced by LPS. , the skin protection, antioxidant and anti-inflammatory composition of the present invention can be usefully applied to cosmetics.

Description

Cosmetic composition for anti-inflammation, anti-oxidation and skin protection against UV comprising biorenovation extract of black tea as effective component}

The present invention relates to a cosmetic composition for anti-inflammatory, antioxidant and skin protection against ultraviolet rays containing black tea bioconversion extract as an active ingredient.

Inflammation is a type of normal body's defense mechanism that occurs when a physical wound or infection occurs with microorganisms. Through this inflammation, pathogens are neutralized or removed and damaged tissues are repaired. It ensures normal structure and function. Most diseases accompanied by inflammation result in a decrease in quality of life such as tissue damage, pain, and itching, and chronic inflammatory conditions include arthritis, asthma, multiple sclerosis of the brain and spinal cord, inflammatory bowel disease, and arteriosclerosis. causes Macrophages are the main cells responsible for innate immunity. They are activated by numerous factors such as cytokines and bacterial lipopolysaccharide (LPS), and activated macrophages produce nitric oxide (NO) and prostaglandin E2 ( It produces inflammatory factors such as prostaglandin E2 and PGE2) as well as pro-inflammatory cytokines such as tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and interleukin-1 (IL-1). NO is a type of free radical and is synthesized by nitric oxide synthase (NOS), and is composed of three isoforms: endothelial NOS (eNOS), neuronal NOS (nNOS), and inducible NOS (iNOS). isoform) exists. Pro-inflammatory cytokines such as IL-1, TNF-α, and interferon-gamma can induce iNOS expression and cause NO production. Many malignant cancers, such as brain cancer, breast cancer, lung cancer, prostate cancer, pancreatic cancer, and melanoma, are associated with iNOS expression, and excessive production of NO can cause epithelial cell carcinoma, mutations, and DNA structural damage.

Biorenovation refers to a method of producing or manufacturing a desired product from precursor materials using the enzymatic function of microorganisms, and is a method of inducing structural changes in materials through biological methods such as microbial fermentation or enzyme treatment. collectively referred to as It is newly known that due to the above changes, the content of active ingredients is increased, absorption rate is improved, and new functional ingredients are created, and the development of materials using these techniques is attracting attention around the world. This bioconversion method is widely used in the production of pharmaceutical raw materials including antibiotics and steroids, as well as useful substances such as amino acids and vitamins. In developed countries, many products targeting high added value are being sold due to the launch of products using biotechnology techniques, and these new biotechnology-based materials have been recognized as safe by consumers, and have recently become a variety of products that systematically combine various biotechnology technologies. Functional materials are emerging.

Meanwhile, black tea is a type of tea that has gone through an oxidation process, unlike white tea, yellow tea, and green tea. Therefore, it has a stronger flavor and contains more caffeine. In the East, the color of the tea is red, so it is called black tea, but in the West, it is called 'black tea' because of the black color of the tea leaves. Black tea is a type of dicotyledonous plant called Camellia sinensis , and is obtained through the processing of leaves obtained from trees that grow mainly in tropical or temperate regions. During the processing process, black tea undergoes a lot of oxidation. Oxidase is a protein component, and when tea leaves are just harvested and put in a cauldron and then heated and rolled, they are unable to catalyze the oxidation process and the leaves remain green. Black tea leaves are harvested and dried, then the crunchy leaves are rubbed or rolled to damage the leaves and destroy the cells. Then, the polyphenol enzymes in the leaves flow out and undergo an oxidation reaction with oxygen in the air, turning brown. This is called black tea. Black tea contains a large amount of theaflamin and polyphenols due to oxidation reactions.

As anti-inflammatory related prior art, Korean Patent No. 1485896 discloses ‘Cosmetic composition for improving antioxidant, anti-inflammatory and atopic dermatitis and manufacturing method thereof using fermented black tea extract as an active ingredient,’ and Korean Patent No. 1412446 discloses ‘Cosmetic composition for improving antioxidant, anti-inflammatory and atopic dermatitis and its manufacturing method. A method for producing an anti-inflammatory cosmetic composition containing green tea callus extract is disclosed. However, the 'cosmetic composition for anti-inflammatory, antioxidant and skin protection against ultraviolet rays containing black tea bioconversion extract as an active ingredient' of the present invention has not yet been disclosed.

The present invention was developed in response to the above-mentioned needs, and provides a cosmetic composition for anti-inflammatory, antioxidant and skin protection against ultraviolet rays containing black tea bioconversion extract as an active ingredient, and provides a black tea bioconversion extract produced through a bioconversion method. It has no cytotoxicity, has superior skin protection activity against ultraviolet rays and antioxidant activity compared to black tea extract, and inhibits the production of NO (nitric oxide), PGE ₂ (prostaglandin E ₂ ), and pro-inflammatory cytokines induced by LPS. The present invention was completed by confirming that it was significantly suppressed.

In order to solve the above problems, the present invention provides a method for preventing or improving skin inflammation containing black tea bioconversion extract as an active ingredient; Skin protection against ultraviolet rays; and a cosmetic composition for antioxidant use.

In addition, the present invention includes the steps of (1) extracting black tea by adding an extraction solvent; (2) mixing the black tea extract obtained in step (1) with a Baillus sp. strain to induce a bioconversion reaction; And (3) centrifuging the bioconversion reactant obtained in step (2) and then obtaining a supernatant; preventing or improving skin inflammation, skin protection against ultraviolet rays, and antioxidant effect. It provides a method for producing a black tea bioconversion extract having a.

The present invention relates to a cosmetic composition for anti-inflammatory, antioxidant and skin protection against ultraviolet rays containing a black tea bioconversion extract as an active ingredient. Specifically, the black tea bioconversion extract prepared through the bioconversion method is non-cytotoxic, and the black tea bioconversion extract is non-cytotoxic. Compared to extracts, it has excellent skin protection and antioxidant activity against ultraviolet rays, and has the effect of significantly suppressing the production of NO (nitric oxide), PGE ₂ (prostaglandin E ₂ ), and pro-inflammatory cytokines induced by LPS. .

Figure 1 shows the results of HPLC analysis of black tea extract and black tea bioconversion extract according to an embodiment of the present invention. NC is a suspension of Bacillus sp. strain JD3-7.
Figure 2 shows the skin cell protection effect of black tea extract and black tea bioconversion extract against ultraviolet rays according to an embodiment of the present invention. * indicates a statistically significant increase in the skin cell protection effect of black tea bioconversion extract compared to black tea extract, p<0.05.
Figure 3 shows the DPPH radical scavenging activity of black tea extract and black tea bioconversion extract according to an embodiment of the present invention. PC is 1% ascorbic acid, a positive control, and ** indicates a statistically significant increase in the DPPH radical scavenging activity of the black tea bioconversion extract compared to the black tea extract, p<0.01.
Figure 4 shows the superoxide anion radical scavenging activity of black tea extract and black tea bioconversion extract according to an embodiment of the present invention. PC is 1% ascorbic acid, a positive control, ** and *** indicate a statistically significant increase in superoxide anion radical scavenging activity of black tea bioconversion extract compared to black tea extract, ** is p <0.01, and *** is p<0.001.
Figure 5 shows the cell survival rate of macrophages according to treatment with black tea extract and black tea bioconversion extract according to an embodiment of the present invention.
Figure 6 shows the effect of inhibiting NO (nitric oxide) production of black tea extract and black tea bioconversion extract according to an embodiment of the present invention. ** and *** indicate a statistically significant increase in the NO production inhibition effect of the black tea bioconversion extract compared to the black tea extract, ** is p < 0.01, and *** is p < 0.001.
Figure 7 shows the inhibitory effect of black tea extract and black tea bioconversion extract on PGE ₂ (prostaglandin E ₂ ) production according to an embodiment of the present invention. * indicates a statistically significant increase in the inhibition effect of PGE ₂ production of black tea bioconversion extract compared to black tea extract, p<0.05.
Figure 8 shows the inhibitory effect of black tea extract and black tea biotransformation extract on IL-6 production according to an embodiment of the present invention. * indicates a statistically significant increase in the IL-6 production inhibition effect of black tea bioconversion extract compared to black tea extract at a treatment concentration of 0.25 mg/ml, p<0.05.

In order to achieve the purpose of the present invention, the present invention provides a method for preventing or improving skin inflammation containing black tea bioconversion extract as an active ingredient; Skin protection against ultraviolet rays; and a cosmetic composition for antioxidant use.

The term "bioconversion" or "bioconversion method" used in the present invention refers to a method of producing and manufacturing a compound that induces structural changes such as phosphorylation and glycosylation from a substrate or precursor using microorganisms. means. The phosphorylation means binding to the substrate by replacing a phosphoric acid group (HOPO(OH)O-) with the hydrogen atom of the substrate by the action of a phosphorylation enzyme, and the glycosylation involves binding to the substrate as a monosaccharide, This means that glucosides such as polysaccharides are added. The black tea bioconversion extract can increase the content of active ingredients present in black tea by bioconversion by culturing the black tea extract with the strain, and the functional groups of useful compounds contained in black tea are replaced to create new compounds that did not previously exist. can induce the creation of .

The black tea bioconversion extract can be prepared by a method comprising the following steps, but is not limited to this:

(1) Extracting black tea by adding an extraction solvent;

(2) mixing the black tea extract obtained in step (1) with microorganisms to induce a bioconversion reaction; and

(3) Centrifuging the bioconversion reactant obtained in step (2) and obtaining a supernatant.

In step (1), the extraction solvent is preferably selected from water, lower alcohols of C ₁ to C ₄ , or mixtures thereof, and more preferably ethanol, but is not limited thereto. In step (2), the microorganism may preferably be a Bacillus sp. strain, more preferably a Bacillus sp. JD3-7 strain, but is not limited thereto.

In addition, the black tea bioconversion extract of the present invention is characterized by improved antioxidant activity, skin protection activity against ultraviolet rays, and anti-inflammatory activity compared to black tea extract.

In the cosmetic composition according to one embodiment of the present invention, the black tea bioconversion extract may be included in an amount of 0.01 to 20% by weight, preferably, and 0.5 to 10% by weight, based on the total weight of the cosmetic composition, and is most preferably included. It may be included at 1 to 5% by weight, but is not limited thereto.

The cosmetic composition of the present invention includes ointment for external use, cream, softening lotion, nourishing lotion, pack, essence, hair tonic, shampoo, rinse, hair conditioner, hair treatment, gel, skin lotion, skin softener, skin toner, astringent, milk. A group consisting of lotion, moisture lotion, nutritional lotion, massage cream, nutritional cream, eye cream, moisture cream, hand cream, foundation, nutritional essence, sunscreen, soap, cleansing foam, cleansing lotion, cleansing cream, body lotion and body cleanser. It is preferably prepared in any one formulation selected from. A cosmetic composition composed of each of these formulations may contain various bases and additives necessary and appropriate for the formulation of the formulation, and the types and amounts of these components can be easily selected by a person skilled in the art.

When the formulation of the cosmetic composition of the present invention is a paste, cream, or gel, animal fiber, plant fiber, wax, paraffin, starch, tragacanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, or zinc oxide are used as carrier ingredients. etc. can be used.

When the formulation of the cosmetic composition of the present invention is powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, or polyamide powder can be used as the carrier ingredient, and especially in the case of spray, chlorofluoro It may contain propellants such as hydrocarbon, propane-butane or dimethyl ether.

When the formulation of the cosmetic composition of the present invention is a solution or emulsion, a solvent, solvating agent or emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene. These include fatty acid esters of glycol, 1,3-butylglycol oil, glycerol aliphatic esters, polyethylene glycol or sorbitan.

When the formulation of the cosmetic composition of the present invention is a suspension, the carrier component includes water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester, and polyoxyethylene sorbitan ester, Microcrystalline cellulose, aluminum metahydroxide, bentonite, agar, or tracant may be used.

When the formulation of the cosmetic composition of the present invention is a surfactant-containing cleansing agent, the carrier ingredients include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, acethionate, imidazolinium derivative, methyl taurate, and sarcosinate. , fatty acid amide ether sulfate, alkylamidobetaine, fatty alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative, or ethoxylated glycerol fatty acid ester can be used.

The cosmetic composition of the present invention may further contain excipients including fluorescent substances, fungicides, hydrotropes-inducing substances, fragrances, fragrance carriers, proteins, solubilizers, sugar derivatives, sunscreens, vitamins, plant extracts, etc.

In addition, the present invention

(1) Extracting black tea by adding an extraction solvent;

(2) mixing the black tea extract obtained in step (1) with a Baillus sp. strain to induce a bioconversion reaction; and

(3) centrifuging the bioconversion reactant obtained in step (2) and then obtaining a supernatant; preventing or improving skin inflammation, protecting the skin against ultraviolet rays, and providing antioxidant effects. A method for producing a black tea bioconversion extract is provided.

In the method for producing a black tea bioconversion extract of the present invention, the extraction solvent is preferably selected from water, C ₁ -C ₄ lower alcohol, or a mixture thereof, more preferably ethanol, but is not limited thereto. In step (2), the Bacillus sp. strain may preferably be a Bacillus sp. JD3-7 strain, but is not limited thereto.

Hereinafter, the present invention will be described in detail through examples. However, the following examples only illustrate the present invention, and the content of the present invention is not limited to the following examples.

Preparation Example 1. Preparation of black tea extract

Add 1 liter of 70% (v/v) ethanol to 150 g of dried black tea and extract at room temperature for 24 hours to recover the primary extract. Add 1 liter of 70% (v/v) ethanol and extract at room temperature for 24 hours. After extracting and recovering the secondary extract, the recovered primary and secondary extracts were mixed and filtered through a filter (TOYO, Japan) with a pore size of 5.0㎛ to obtain a black tea extract. The filtered extract was prepared by vacuum concentration to 5 Brix or more.

Preparation Example 2. Preparation of black tea bioconversion extract

The microorganism for biorenovation was the Bacillus JD3-7 ( Bacillus sp. JD3-7, KACC 92346P) strain isolated from traditional soybean sauce from Jeju Island, and cultured at 30°C using NB (Nutrient broth) medium. was cultured for 20 hours. Afterwards, the cells recovered by centrifugation at 5,000 rpm for 10 minutes were washed twice with PG buffer (50mM Phosphate buffer pH 7.2, 2% Glycerin) and suspended in 5 ml of PG buffer. The black tea of Preparation Example 1 was added to the flask. The bioconversion reaction was performed under the same conditions at 30°C for 72 hours by adding the extract and suspension of the Bacillus JD3-7 strain. After completion of the reaction, the supernatant was collected by centrifugation, concentrated under reduced pressure, and dissolved in water to prepare a black tea bioconversion extract.

Example 1. HPLC analysis

The black tea extract and black tea bioconversion extract prepared in Preparation Examples 1 and 2 were tested for production of bioconversion compounds through high performance liquid chromatography (HPLC) analysis.

Specifically, a Prominence HPLC System (Shimadzu, Japan) device was used, with LC-2030/2040 PDA Detector as a detector and Shim-pack GIS as a column. 0.1% TFA and acetonitrile were used as solvents, and analysis conditions were 10% to 100% acetonitrile for 30 minutes. The wavelength was set to 254 nm, the flow rate was set to 1 mL/min, and the injection volume was 10 μl.

As a result, as shown in Figure 1, it was confirmed that the black tea bioconversion extract contained a number of peaks that did not exist in the black tea extract, and that the intensity of some peaks became stronger.

Example 2. Skin cell protection effect

The black tea extract and black tea bioconversion extract prepared in Preparation Examples 1 and 2 were used at a concentration of 0.25 and 0.5 mg/ml, and the cells were CCD-1064sk human fibroblast cell line and RAW purchased from ATCC. 264.7 cells were used. IMDM (Iscove's modified Dulbeco's medium) supplemented with 10% FBS (fetal bovine serum) and 1% penicillin-streptomycin was used as a culture medium for CD-1064sk human fibroblasts, and RAW 264.7 cells were cultured. As a medium, DMEM (Dulbecco's Modified Eagle Medium) supplemented with 10% FBS (fetal bovine serum) and 1% penicillin-streptomycin was used. Each of the cells was cultured in a 10-cm cell culture dish with 10 ml of medium in an incubator at 37°C and 5% CO ₂ conditions until the cells reached 70-80% confluency. The medium was changed twice a week, and cells that reached confluence were trypsinized using trypsin-EDTA and maintained by subculture.

To measure the skin cell protection effect, the medium was removed from the cell culture, washed twice with PBS buffer, and then measured with a UVA/UVB meter 850009 (Sper scientific, Scottsdale, AZ, USA) to prevent the cells from drying out during ultraviolet irradiation. 1 ml of PBS was dispensed into the culture vessel, and then UVB was irradiated at doses of 50, 100, 200, and 300 mJ/cm2. Afterwards, black tea extract and black tea bioconversion extract were treated for 1 hour at concentrations of 0.25 and 0.5 mg/ml under the optimized conditions of UVB 200mJ/cm2 and then irradiated with UVB 200mJ/cm2. After washing once with 1 ml of PBS buffer, the black tea extract and black tea biotransformation extract were exchanged for culture medium added at concentrations of 0.25 and 0.5 mg/ml. Cells were irradiated with 200 mJ/cm2 of UVB and 4 hours later, cell viability was measured through MTT analysis. A 5 mg/ml MTT solution dissolved in PBS buffer was diluted 10-fold in new medium, and 100 μl of this solution was added to each cell well and incubated at 37°C for 4 hours. The culture supernatant was removed, 200㎕ of DMSO (dimethyl sulfoxide) was added to each well to dissolve the MTT-formazan crystals generated in the cells, and then measured at 540㎚ using an absorption spectrophotometer (UVmax, Molecular Device, USA). Absorbance was measured. The average absorbance for each sample group was measured, and cytotoxicity was evaluated by comparing it with the absorbance measurement of the control group.

As a result, when cell viability was measured after irradiation with UVB (200 mJ/㎠) and treatment with black tea extract and black tea bioconversion extract for 1 hour as shown in Figure 2, cells in the group treated with UVB alone compared to the untreated control group were Survival rate was significantly reduced. On the other hand, the cell survival rate increased in a concentration-dependent manner in both the black tea extract and black tea bioconversion extract treatment groups, and the cell survival rate in the black tea bioconversion extract treatment group was statistically significantly increased compared to the black tea extract.

Example 3. Antioxidant activity

(1) DPPH (1,1-diphenyl-2 picrylhydrazyl) radical scavenging activity

DPPH (D9132, SIGMA) was dissolved in a mixed solvent of methanol and distilled water 6:4 (v/v) to prepare a DPPH solution, and then tested by adjusting the absorbance value to about 0.6 at 513 nm. 2 ml of DPPH solution was mixed with each of the black tea extract and black tea bioconversion extract prepared in Preparation Examples 1 and 2, reacted at room temperature for 1 hour, and then measured for absorbance at 513 nm using a spectrophotometer (UVmax, Molecular Device, USA). was measured. 1% ascorbic acid was used as a positive control.

As a result, as shown in Figure 3, the DPPH radical scavenging activity of the black tea extract and the black tea bioconversion extract increased in a concentration-dependent manner, and the DPPH radical scavenging activity of the black tea bioconversion extract increased statistically significantly compared to the black tea extract.

(2) Superoxide anion radical scavenging activity

Superoxide anion radical scavenging activity was measured by the NBT (nitro blue tetrazolium) reduction method. Prepare a substrate/reaction solution by adding 1.6 ml of Triton did. An enzyme solution was prepared by dissolving 12㎕ of xanthine oxidase in 10㎖ of 300mM PBS buffer (pH 7.8). 1 ml of buffer was placed in the cell of the spectrophotometer, and then 0.5 ml of the substrate/reaction solution prepared above was added. Afterwards, the black tea extract and the black tea bioconversion extract prepared in Preparation Examples 1 and 2 were prepared at various concentrations and treated at 100 ㎕ each. 0.5 mL of the enzyme solution prepared above was added and reacted at 37°C for 30 minutes. Absorbance was measured at 540 nm.

As a result, as shown in Figure 4, the superoxide anion radical scavenging activity of the black tea extract and the black tea bioconversion extract increased in a concentration-dependent manner, and the superoxide anion radical scavenging activity of the black tea bioconversion extract was statistically significant compared to the black tea extract. It increased further.

Example 4. Cytotoxicity

The cytotoxicity of black tea extract and black tea biotransformation extract against RAW264.7 cells was measured through cell viability analysis using the MTT assay. Using DMEM (Dulbecco's Modified Eagle Medium) supplemented with 10% FBS (fetal bovine serum), the cells were distributed at a concentration of ^8.0 The bioconversion extract and LPS (1㎍/㎖) were treated simultaneously and reacted for 24 hours. Afterwards, MTT (Thiazolyl Blue Tetrazolium Bromide) reagent was added to each well and reacted at 37°C for 4 hours. After removing the supernatant, DMSO was added to dissolve the formed Formazan blue, and then ELISA reader was used. Absorbance was measured at 570 nm using

As a result, as shown in Figure 5, black tea extract and black tea bioconversion extract showed a cell viability of more than 95% at all treatment concentrations.

Example 5. Anti-inflammatory effect

(1) Inhibition of NO (Nitric oxide) production

RAW 264.7 cells were seeded at a concentration ^of 8.0 Pre-cultured for 18 hours in an incubator under ₂ conditions. Afterwards, black tea extract or black tea bioconversion extract and LPS (1 μg/ml) were simultaneously treated and cultured for 24 hours. The culture was then centrifuged at 10,000 rpm for 3 minutes to remove precipitates, and the supernatant was recovered. 100 ㎕ of the recovered supernatant and 100 ㎕ of Greek reagent [1% (w/v) sulfanilamide, 0.1% (w/v) naphylethylenediamine in 2.5% (v/v) phosphoric acid] were mixed in a 96-well plate for 10 minutes. After dark reaction, absorbance was measured at 540 nm.

As a result, as shown in Figure 6, NO production was significantly increased in the LPS-only treated group compared to the untreated control group. On the other hand, NO production was reduced in a concentration-dependent manner in the black tea extract treatment group and the black tea bioconversion extract treatment group compared to the LPS-only treatment group, and the black tea bioconversion extract significantly reduced NO production compared to the existing black tea extract.

(2) PGE ₂ (Prostaglandin E ₂ ) and inhibition of production of pro-inflammatory cytokines.

Using DMEM (Dulbecco's Modified Eagle Medium) supplemented with 10% FBS (fetal bovine serum), RAW ^264.7 cells were distributed at _a concentration of 8.0 After pre-culturing in an incubator for 18 hours, black tea extract or black tea biotransformation extract and LPS (1 μg/ml) were simultaneously treated and cultured for 24 hours. Afterwards, the culture was centrifuged at 12,000 rpm for 3 minutes to remove precipitates, and the supernatant was recovered. PGE ₂ in the recovered supernatant The content was measured using an ELISA kit (Mouse PGE ₂ , R&D Systems, USA), and the content of proinflammatory cytokine IL-6 (Interleukin-6) was measured using a mouse IL-6 ELISA kit (San diego, USA). Measured.

As a result, as shown in Figures 7 and 8, the production of PGE ₂ and IL-6 was significantly increased in the LPS-only treated group compared to the untreated control group. On the other hand, in the black tea extract treatment group and the black tea bioconversion extract treatment group, the production of PGE ₂ and IL-6 was suppressed in a concentration-dependent manner compared to the LPS-only treatment group, and the black tea bioconversion extract produced PGE ₂ and IL-6 compared to the existing black tea extract. -6 production was significantly reduced.

Formulation Example 1-3. Soft lotion (skin)

According to the composition disclosed in Table 1 below, the mixture was sufficiently dispersed, moistened and mixed to form a uniform gel, then neutralized by adding a neutralizer, and then added with cosmetic ingredients and stirred to solubilize, and then stirred to ensure uniform distribution. Soft lotion (skin) containing the black tea bioconversion extract of the present invention was prepared in a container.

Soft lotion (skin) ingredients ingredient Formulation Example 1 Formulation Example 2 Formulation Example 3 Content (% by weight) Content (% by weight) Content (% by weight) Black tea bioconversion extract 5.00 2.50 1.00 Butylene glycol 2.00 2.00 2.00 glycerin 3.00 3.00 3.00 Oleyl alcohol 2.00 2.00 2.00 Polysorbate 20 1.00 1.00 1.00 ethanol 6.00 6.00 6.00 antiseptic 0.5 0.5 0.5 incense 0.5 0.5 0.5 Purified water remaining amount remaining amount remaining amount Sum 100.00 100.00 100.00

Formulation Example 4-6. Nutritional emulsion (milk lotion)

According to the composition disclosed in Table 2 below, the mixture was sufficiently dispersed, moistened and mixed to form a uniform gel, then neutralized by adding a neutralizer, and then added with cosmetic ingredients and stirred to solubilize, and then stirred to ensure uniform distribution. A nutritional emulsion (milk lotion) containing the black tea bioconversion extract of the present invention was prepared in a container.

Nutritional emulsion (milk lotion) ingredients ingredient Formulation Example 4 Formulation Example 5 Formulation Example 6 Content (% by weight) Content (% by weight) Content (% by weight) Black tea bioconversion extract 5.00 2.50 1.00 Butylene glycol 4.00 4.00 4.00 glycerin 3.00 3.00 3.00 Oleyl alcohol 1.00 1.00 1.00 Polysorbate 20 1.00 1.00 1.00 antiseptic 0.5 0.5 0.5 incense 0.5 0.5 0.5 Purified water remaining amount remaining amount remaining amount Sum 100.00 100.00 100.00

Formulation Example 7-9. cream

According to the composition disclosed in Table 3 below, the mixture was sufficiently dispersed, moistened and mixed to form a uniform gel, then neutralized by adding a neutralizer, and then added with cosmetic ingredients and stirred to solubilize, and then stirred to ensure uniform distribution. A cream containing the black tea bioconversion extract of the present invention was prepared in a container.

Cream formulation ingredients ingredient Formulation Example 7 Formulation Example 8 Formulation Example 9 Content (% by weight) Content (% by weight) Content (% by weight) Black tea bioconversion extract 5.00 2.50 1.00 Butylene glycol 2.00 2.00 2.00 glycerin 4.00 4.00 4.00 Potassium Hydroxide 0.10 0.10 0.10 tocopheryl acetate 1.0 1.0 1.0 liquid paraffin 5.0 5.0 5.0 squalane 5.0 5.0 5.0 Macadimia nut oil 10.0 10.0 10.0 Polysorbate 60 1.0 1.0 1.0 Sorbitan sesquioleate 1.0 1.0 1.0 carbomer 0.3 0.3 0.3 antiseptic 0.5 0.5 0.5 incense 0.5 0.5 0.5 Purified water remaining amount remaining amount remaining amount Sum 100.00 100.00 100.00

Claims (8)

A cosmetic composition containing black tea bioconversion extract as an active ingredient for preventing or improving skin inflammation, protecting skin against ultraviolet rays, and providing antioxidant properties. The method of claim 1, wherein the black tea bioconversion extract is
(1) Extracting black tea by adding an extraction solvent;
(2) mixing the black tea extract obtained in step (1) with microorganisms to induce a bioconversion reaction; and
(3) centrifuging the bioconversion reactant obtained in step (2) and obtaining a supernatant; a cosmetic for preventing or improving skin inflammation, protecting the skin against ultraviolet rays, and providing antioxidant properties, comprising: Composition. The cosmetic of claim 2, wherein the extraction solvent in step (1) is water, a lower alcohol of C ₁ to C ₄ , or a mixture thereof, for preventing or improving skin inflammation, protecting skin against ultraviolet rays, and for antioxidant purposes. Composition. The cosmetic composition according to claim 2, wherein the microorganism in step (2) is a strain of the genus Bacillus ( Baillus sp.), preventing or improving skin inflammation, protecting the skin against ultraviolet rays, and providing antioxidant properties. The method of claim 1, wherein the black tea biotransformation extract has improved antioxidant activity, skin protection activity against ultraviolet rays, and anti-inflammatory activity compared to the black tea extract, preventing or improving skin inflammation, skin protection against ultraviolet rays, and antioxidant activity. Cosmetic composition for use. The cosmetic composition according to claim 1, wherein the black tea bioconversion extract is contained in an amount of 0.01 to 20% by weight based on the total weight of the cosmetic composition. The method of claim 1, wherein the composition includes an ointment for external use, cream, softening lotion, nourishing lotion, pack, essence, hair tonic, shampoo, rinse, hair conditioner, hair treatment, gel, skin lotion, skin softener, skin toner, Astringent, milk lotion, moisture lotion, nutritional lotion, massage cream, nutritional cream, eye cream, moisture cream, hand cream, foundation, nutritional essence, sunscreen, soap, cleansing foam, cleansing lotion, cleansing cream, body lotion and body cleanser. A cosmetic composition for preventing or improving skin inflammation, protecting skin against ultraviolet rays, and antioxidant, characterized in that it is prepared in any one formulation selected from the group consisting of. (1) Extracting black tea by adding an extraction solvent;
(2) mixing the black tea extract obtained in step (1) with a Baillus sp. strain to induce a bioconversion reaction; and
(3) centrifuging the bioconversion reactant obtained in step (2) and then obtaining a supernatant; preventing or improving skin inflammation, protecting the skin against ultraviolet rays, and providing antioxidant effects. Method for producing black tea bioconversion extract.