KR20230136290A - extraction method of lignoceric acid from purslane using super critical method and manufacturing method of health food using thereof - Google Patents

Abstract

The present invention relates to a method for extracting lignoceric acid, which consists of a first step of mixing purslane powder and perilla seed powder to produce a purslane perilla mixture, a second step of extracting the purslane perilla mixture, and the extracted purslane perilla seed mixture. A third step of milking the mixture, a fourth step of removing the sediment of the milked purslane and perilla seed mixture to obtain a purslane and perilla mixed extract, and a fifth step of extracting lignoceric acid from the purslane and perilla mixed extract. It is characterized by

Description

Method of extracting lignoceric acid from purslane using super critical method and manufacturing method of health food using the same {extraction method of lignoceric acid from purslane using super critical method and manufacturing method of health food using the same}

The present invention relates to a method of extracting lignoceric acid from purslane using a supercritical method and a method of manufacturing health food using the same. More specifically, the present invention relates to a method of extracting lignoceric acid from purslane using a supercritical method and a method of producing health food using the same. It is possible to extract lignoceric acid and it relates to a method of extracting lignoceric acid from purslane using a supercritical method, which can be used in health foods, and a method of manufacturing health foods using the same.

Purslane is an annual herb of the Purslane family, and is also called as Five Elements, Jangmyeongchae, Portulaca and Portulaca, etc. It mainly grows on roadsides, empty lots, and field banks, grows about 30 cm in size, and blooms in summer. In general, purslane was mixed with seasoning and used as an edible or medicinal ingredient, but recently, as its many functional properties have become known, it has been developed and used as a treatment for hyperglycemia, a beverage with excellent anticancer and antibacterial effects, a nicotine and tar remover, a food preservative, and a health food for hair growth.

In general, purslane contains lignoceric acid, but purslane itself has poor flow properties and has problems such as the components being destroyed during extraction, which prevents it from being sufficiently extracted to be used in health foods.

Republic of Korea Patent Publication No. 10-2012-0124190

In order to solve the above-mentioned problems, the purpose of the present invention is to mix perilla seeds and use a supercritical carbon dioxide extraction method to extract high-quality lignoceric acid due to its good flowability, and to extract high-quality lignoceric acid from purslane, which can be used in health foods. It relates to a method of extracting lignoceric acid by the critical method and a method of manufacturing health food using it.

In order to achieve the above-described object, the present invention, a method of extracting lignoceric acid from purslane by a supercritical method and a method of manufacturing health food using the same, includes the first step of mixing purslane powder and perilla seed powder, the mixed A second step of extracting purslane powder and perilla seed powder using a supercritical carbon dioxide extraction method to produce a purslane perilla mixed extract, a third step of milking the purslane perilla mixed extract, and a fourth step of removing sediment from the milked purslane perilla mixed extract. It is characterized by comprising a fifth step of extracting lignoceric acid from the purslane and perilla mixed extract from which the precipitate has been removed.

The first step is characterized by mixing 1 part by weight of perilla seed powder with 1 part by weight of purslane powder.

The second stage extraction is performed at a temperature of 60°C or lower and a pressure of 300 to 500 bar, and CO ₂ is injected at a flow rate of 3L/min.

It is characterized in that health food is manufactured by using lignoceric acid extracted in the same manner as above and mixing the lignoceric acid with perilla oil.

A health food is prepared by mixing 95 to 105 parts by weight of perilla oil with 3 parts by weight of lignoceric acid.

The method of extracting lignoceric acid from purslane by a supercritical method according to the present invention and the method of manufacturing health food using the same have the following effects. By mixing perilla seeds and using the supercritical carbon dioxide extraction method, high-quality lignoceric acid can be extracted due to its good flowability, which has the advantage of being used in health foods.

Figure 1 is a flowchart of a method for extracting lignoceric acid from purslane by a supercritical method according to the present invention.
Figure 2 is a human test report of the purslane perilla mixed extract using lignoceric acid, the present invention.
Figure 3 is a human test report of the purslane perilla mixed extract using lignoceric acid, the present invention.
Figure 4 is a human test report of the purslane perilla mixed extract using lignoceric acid, the present invention.
Figure 5 is a human test report of the purslane perilla mixed extract using lignoceric acid, the present invention.
Figure 6 is a human test report of the purslane perilla mixed extract using lignoceric acid, the present invention.
Figure 7 is a human test report of the purslane perilla mixed extract using lignoceric acid, the present invention.
Figure 8 is a human test report of the purslane perilla mixed extract using lignoceric acid, the present invention.
Figure 9 is a human test report of the purslane perilla mixed extract using lignoceric acid, the present invention.
Figure 10 is a human test report of the purslane perilla mixed extract using lignoceric acid, the present invention.

Hereinafter, a preferred embodiment of the method of extracting lignoceric acid from purslane by a supercritical method according to the present invention and the method of manufacturing health food using the same will be described in detail with reference to the attached drawings.

First, the lignoceric acid extraction method of the present invention will be described in detail. The lignoceric acid extraction method of the present invention includes a first step (S1) of mixing purslane powder and perilla seed powder, and a second step of extracting the mixed purslane powder and perilla seed powder using a supercritical carbon dioxide extraction method to produce a purslane perilla mixed extract. Step (S2), the third step (S3) of milking the purslane and perilla mixed extract, the fourth step (S4) of removing the sediment of the milked purslane and perilla mixed extract, the league from the purslane and perilla mixed extract from which the sediment has been removed. It is characterized by comprising a fifth step (S5) of extracting noceric acid.

First, the lignoceric acid extraction method of the present invention includes a first step (S1) of mixing purslane powder and perilla seed powder.

Purslane is an annual herb belonging to the Purslane family and commonly grows in fields or along roadsides. It has strong vitality and can survive for a long time even if it is uprooted. As a herbal medicine, whole plant is used and it is called portulaca hyeon. It is said that skin nutrients such as skin lotion are also made from portulaca string. It contains a large amount of mineral salts such as calcium oxalate and dopamine, so it is used as a detoxifier and diuretic in Korea and China, and in the West it is used for dry stomach, asthma, and cystitis. It is non-poisonous and cold in nature. When eaten, only a lot of juice comes out and there is no taste, so it is usually cooked with soybean paste or red pepper paste and eaten as a vegetable. Even in the West, it is a vegetable that has its origins since Roman times and is often eaten as a salad or boiled. The juice has a slightly viscous consistency, and since ancient times, purslane has been pounded and used as a natural sunscreen or to treat sunburn.

Perilla is an annual plant or seed of the Lamiaceae family. The leaves are called perilla leaves, and the oil extracted from the seeds is called perilla oil. You can use perilla oil by squeezing it out, or fry the seeds without squeezing the oil and grind them into powder to use as a seasoning or garnish. Perilla oil contains vitamin F, which is said to soothe these conditions and create fair skin. In addition, perilla oil contains ingredients that promote hematopoiesis, which prevents blood from clumping in the blood vessels and helps it flow smoothly, creating an environment for good blood circulation. The efficacy of perilla oil also has a positive effect on growing teenagers. It contains DHA, which affects brain development, so it is good for growing children and good for preventing dementia in the elderly. It is also said that consuming perilla oil can suppress gray hair. It is said to supply good nutrients to the hair roots, making them stronger and preventing gray hair from growing. In addition, it is said that Omega 3 can be obtained through the powerful effects of perilla oil. It is said that among many cooking oils, perilla oil contains the largest amount of this ingredient. It is said that if consumed regularly, it dilates blood vessels, develops brain nerves, prevents dementia, and prevents the development of breast cancer. Also, perilla oil is said to contain a lot of unsaturated fatty acids, which are fats that are helpful to our body. Because it is used in seasoned vegetables that are good for your health, you can have healthy eating habits and it is also said to be good for preventing adult diseases that are common in Korea. It is said that absorption of vitamins is improved when eaten with wild greens. Lastly, it is said that perilla oil improves allergic constitution. This is because it contains a lot of alpha-linolenic acid. According to recent research results, it has shown good results in various allergic reactions. It is said that it can improve the inconveniences of various allergic diseases that arise as urban living increases.

Purslane and perilla seeds, which have the above-mentioned efficacy, are characterized by being prepared as dried powder. This may be purslane and perilla seeds that are harvested, washed and naturally dried, placed in a far-infrared dryer below 50°C, and dried for more than 24 hours. This is limited to this. It is possible to use it as long as it is a dry powder.

The purslane powder and perilla seed powder prepared as above are characterized by mixing 1 part by weight of the perilla powder with respect to 1 part by weight of the purslane powder. This is because, if one attempts to extract lignoceric acid using only the purslane powder, it is difficult to proceed with milking due to the sticky viscosity of the purslane powder. Therefore, the perilla powder is added to lower the viscosity and improve flowability to extract lignoceric acid. That is, if the perilla powder is included in less than 1 part by weight relative to 1 part by weight of the purslane powder, the viscosity is not sufficiently lowered, so the flowability is not good, so the subsequent milking step cannot proceed properly, and lignoceric acid cannot be sufficiently extracted. It may not be possible. If the perilla powder is included in excess of 1 part by weight based on 1 part by weight of the purslane powder, the perilla powder has a higher content than the purslane powder, and the extraction amount of lignoceric acid is reduced, thereby reducing efficiency.

Next, the second step (S2) is performed to produce a purslane and perilla mixed extract by extracting the mixed purslane powder and perilla seed powder using a supercritical carbon dioxide extraction method.

Here, the extraction in the second step (S2) uses a supercritical carbon dioxide extraction method, and is characterized in that CO ₂ is injected at a flow rate of 3 L/min. The supercritical carbon dioxide extraction method is performed at a temperature of 60°C or lower and is characterized in that it is performed under a pressure of 300 to 500 bar.

Generally, methods for separating essential oils from plants include steam distillation, direct distillation, compression, cold immersion, and organic solvent methods. Existing distillation extraction and solvent extraction methods affect highly volatile substances contained in essential oil by applying heat, and have the disadvantage of reducing the amount of extraction and active ingredients as the active ingredients volatilize during the process of separating the essential oil and solvent after extraction. In addition, there is a problem in that waste and organic solvents generated during the separation process remain, and there is a disadvantage in that the oil flavor and active ingredients are destroyed. Supercritical fluid extraction technology is an environmentally friendly alternative technology to overcome the problems of these existing methods. Supercritical fluid refers to the state of matter at a temperature and pressure above the critical point and refers to a fluid at which liquefaction and gas cannot be distinguished. The density of a supercritical fluid is close to that of a liquid, but its viscosity is low, making it similar to a gas. Because the supercritical fluid has a high density, low viscosity, and greater diffusion ability than liquid, the supercritical fluid extraction method has a variety of application methods and can be extracted efficiently compared to the general solvent extraction method. In the case of supercritical carbon dioxide extraction, it is inert, tasteless, odorless, and harmless, and can be safely used for food and medicine because it is operated at around room temperature. Additionally, when restored to atmospheric pressure, supercritical carbon dioxide can be easily removed from the extract, so there is no problem with residual solvent.

The present invention uses a supercritical carbon dioxide extraction method that has the advantages described above, and is characterized in that CO ₂ is injected at a flow rate of 3 L/min, and is performed at a temperature of 60°C or less and a pressure of 300 to 500 bar. Purslane and perilla seed mixture must be extracted under the above conditions to sufficiently extract 0.3 mg/g or more of lignoceric acid and use it as a health food.

Next, the third step (S3) of milking the purslane and perilla mixed extract is performed.

Here, the milking is performed using a juice-type milking machine and is performed at 18 to 23° C. for 15 minutes. By carrying out milking at the above temperature, milking can proceed sufficiently to extract high-quality lignoceric acid, and problems such as destruction of lignoceric acid do not occur.

Next, the fourth step (S4) is performed to remove sediment from the extracted purslane and perilla mixed extract.

The fourth step (S4) is a step of removing purslane or perilla residue generated in the milking step.

Next, the fifth step (S5) of extracting lignoceric acid from the purslane and perilla mixed extract from which the precipitate has been removed is performed, completing the lignoceric acid extraction method of the present invention.

Here, the method for extracting lignoceric acid is generally the same as the method for extracting lignoceric acid from peanuts, etc.

Next, the method for producing health food using lignoceric acid extracted in the same manner as above will be described in detail.

First, it is characterized in that health food is manufactured by mixing lignoceric acid and perilla oil extracted in the same manner as above.

Here, perilla oil may be the result of preparing perilla powder and milking it at 20°C for 15 minutes using a juice-type expeller, but it can be used without being limited thereto.

A health food is prepared by mixing 95 to 105 parts by weight of perilla oil with 3 parts by weight of lignoceric acid. If the perilla oil is less than 95 parts by weight relative to 3 parts by weight of lignoceric acid, it may not have sufficient flowability and may be difficult to consume as a health food, and if the perilla oil exceeds 105 parts by weight, the perilla oil is included in one intake. Efficiency may decrease due to low lignoceric acid content. In more detail, it is preferable that the perilla oil is included in 97 parts by weight based on 3 parts by weight of lignoceric acid.

Figures 2 to 10 show 3 parts by weight of lignoceric acid extracted by the method of extracting lignoceric acid of the present invention mixed with 97 parts by weight of perilla oil, which is the result of milking at 20°C for 15 minutes using a juice-pressing expeller. , it was named Purslane Perilla Mixed Extract and its effectiveness and safety were evaluated.

The following describes the lignoceric acid content test and sensory evaluation.

First, the lignoceric acid content experiment examines the lignoceric acid content of the following comparative examples and examples.

Next, in order to compare the quality characteristics of health foods, which are the comparative examples and examples below, the sensory evaluation was performed on graduate students of the Department of Food Engineering, whose suitability as an inspector was recognized, after receiving appropriate training for the purpose of this experiment.

Sensory evaluation items were conducted on a 9-point likert scale, with 9 points being very good and 1 point being very bad, based on the following comparative examples and examples of health food flavor, preference immediately after ingestion, and preference 20 minutes after ingestion. , a score of 5 points or less was judged to be bad, a score of more than 5 points but less than 7 points was judged to be average, and a score of 8 points or more was considered good.

(a) Lignoceric acid content test according to the process

[Comparative Example 1]

After harvesting the purslane, it was washed and naturally dried, placed in a far-infrared dryer below 50°C, and dried for more than 24 hours to check the lignoceric acid content.

[Comparative Example 2]

Using perilla seeds and purslane as raw materials, the perilla seeds were milked and pressed, then purslane was mixed and heated and extracted to confirm the lignoceric acid content.

[Example 1]

After harvesting the purslane, wash and naturally dry it, place it in a far-infrared dryer below 50℃ and dry it for more than 24 hours. Harvest 1 part by weight of purslane powder, wash and naturally dry it and place it in a far-infrared dryer below 50℃ for 24 hours. 1 part by weight of perilla powder dried for more than an hour is mixed and extracted using a supercritical carbon dioxide extraction method. The extraction conditions are set at 60°C and 400 bar pressure with CO ₂ flow rate injected at 3 L/min. Afterwards, they were milked at 20°C for 15 minutes using a milking machine, and the sediment was removed to check the lignoceric acid content.

[Example 2]

After harvesting the purslane, wash and naturally dry it, place it in a far-infrared dryer below 50℃ and dry it for more than 24 hours. Harvest 1 part by weight of purslane powder, wash and naturally dry it and place it in a far-infrared dryer below 50℃ for 24 hours. 1 part by weight of perilla powder dried for more than an hour is mixed and extracted using a supercritical carbon dioxide extraction method. The extraction conditions are set at 60°C and 400 bar pressure with CO ₂ flow rate injected at 3 L/min. Afterwards, they are milked at 20.5°C for 15 minutes using a milking machine, the sediment is removed, and the lignoceric acid is extracted. Afterwards, 3 parts by weight of the lignoceric acid was mixed with 97 parts by weight of perilla oil obtained by extracting the oil using a milking machine at 20°C for 15 minutes and removing the sediment to confirm the content of lignoceric acid.

division Perilla seeds present or not Lignoceric acid extraction method Mixture of lignoceric acid and perilla oil Comparative Example 1 × × × Comparative Example 2 ○ heated extraction × Example 1 ○ Supercritical carbon dioxide extraction method × Example 2 ○ Supercritical carbon dioxide extraction method ○

division Comparative Example 1 Comparative Example 2 Example 1 Example 2 Lignoceric acid
Content (mg/g) - 0.03 0.90 0.34

Through Tables 1 and 2, it can be seen that the results of Examples 1 and 2 are good. This is believed to be a result of process differences.

In the case of Comparative Example 1, only purslane was included, and it was determined that lignoceric acid was not extracted because the purslane was simply dried and used without extraction.

In the case of Comparative Example 2, it was determined that less lignoceric acid was extracted than in the present invention due to problems such as destruction of lignoceric acid due to the heat extraction method.

Therefore, by extracting lignoceric acid using a supercritical carbon dioxide extraction method as in Examples 1 and 2, mixing it with perilla oil and using it as a food, a sufficient amount of lignoceric acid is obtained to have a positive effect on the human body when ingested. May contain ceric acid.

(b) Lignoceric acid content test and sensory evaluation according to the content of purslane powder and perilla seed powder

[Comparative Example 3]

After harvesting the purslane, wash and naturally dry it, place it in a far-infrared dryer below 50℃ and dry it for more than 24 hours. Harvest 1 part by weight of purslane powder, wash and naturally dry it and place it in a far-infrared dryer below 50℃ for 24 hours. Mix 0.5 parts by weight of perilla powder dried for more than an hour, and extract them using a supercritical carbon dioxide extraction method. The extraction conditions are set at 60°C and 400 bar pressure with CO ₂ flow rate injected at 3 L/min. Afterwards, they are milked at 20.5°C for 15 minutes using a milking machine, the sediment is removed, and the lignoceric acid is extracted. Afterwards, 3 parts by weight of the lignoceric acid was mixed with 97 parts by weight of perilla oil obtained by extracting the oil using a milking machine at 20°C for 15 minutes and removing the sediment to confirm the content of lignoceric acid.

[Example 3]

After harvesting the purslane, wash and naturally dry it, place it in a far-infrared dryer below 50℃ and dry it for more than 24 hours. Harvest 1 part by weight of purslane powder, wash and naturally dry it and place it in a far-infrared dryer below 50℃ for 24 hours. 1 part by weight of perilla powder dried for more than an hour is mixed and extracted using a supercritical carbon dioxide extraction method. The extraction conditions are set at 60°C and 400 bar pressure with CO ₂ flow rate injected at 3 L/min. Afterwards, they are milked at 20.5°C for 15 minutes using a milking machine, the sediment is removed, and the lignoceric acid is extracted. Afterwards, 3 parts by weight of the lignoceric acid was mixed with 97 parts by weight of perilla oil obtained by extracting the oil using a milking machine at 20°C for 15 minutes and removing the sediment to confirm the content of lignoceric acid.

[Comparative Example 4]

After harvesting the purslane, wash and naturally dry it, place it in a far-infrared dryer below 50℃ and dry it for more than 24 hours. Harvest 1 part by weight of purslane powder, wash and naturally dry it and place it in a far-infrared dryer below 50℃ for 24 hours. Mix 1.5 parts by weight of perilla powder dried for more than an hour and extract them using a supercritical carbon dioxide extraction method. The extraction conditions are set at 60°C and 400 bar pressure with CO ₂ flow rate injected at 3 L/min. Afterwards, they are milked at 20.5°C for 15 minutes using a milking machine, the sediment is removed, and the lignoceric acid is extracted. Afterwards, 3 parts by weight of the lignoceric acid was mixed with 97 parts by weight of perilla oil obtained by extracting the oil using a milking machine at 20°C for 15 minutes and removing the sediment to confirm the content of lignoceric acid.

division 1 part by weight of perilla seeds to 1 part by weight of purslane Comparative Example 3 0.5 Example 3 One Comparative Example 4 1.5

division Lignoceric acid
Content (mg/g) Sensory evaluation incense Immediately after ingestion
preference 20 minutes after ingestion
preference Comparative Example 3 0.35 Good Good bad Example 3 0.34 good good good Comparative Example 4 0.18 good good good

As shown in Tables 3 and 4, it can be confirmed that the results of Example 3 are good. This is believed to be a result of the difference in parts by weight of perilla seeds relative to 1 part by weight of purslane.

First, in the case of Comparative Example 3, it was judged that the lignoceric acid content was not significant compared to Example 3 because it could not be sufficiently milked due to sticky viscosity. In addition, the fragrant aroma of perilla seeds is less expressed, and it is believed that preference decreases after ingestion due to its somewhat sticky form.

In the case of Comparative Example 4, the taste of perilla seeds? The sensory evaluation results are good due to the scent and non-sticky form, but the lignoceric acid content is judged to be lower due to the inclusion of more perilla seeds than purslane.

Therefore, as in Example 3, if lignoceric acid is extracted by adding 1 part by weight of perilla seeds to 1 part by weight of purslane and used as a health food, both the lignoceric acid content value and the sensory evaluation results are good. .

(c) Lignoceric acid content test according to supercritical carbon dioxide extraction method

[Comparative Example 5]

After harvesting the purslane, wash and naturally dry it, place it in a far-infrared dryer below 50℃ and dry it for more than 24 hours. Harvest 1 part by weight of purslane powder, wash and naturally dry it and place it in a far-infrared dryer below 50℃ for 24 hours. Mix 1 part by weight of perilla powder dried for more than an hour and extract them using a supercritical carbon dioxide extraction method. The extraction conditions are set at 60°C and 200 bar pressure with CO ₂ flow rate injected at 3 L/min. Afterwards, they are milked at 20.5°C for 15 minutes using a milking machine, the sediment is removed, and the lignoceric acid is extracted. Afterwards, 3 parts by weight of the lignoceric acid was mixed with 97 parts by weight of perilla oil obtained by extracting the oil using a milking machine at 20°C for 15 minutes and removing the sediment to confirm the content of lignoceric acid.

[Example 4]

After harvesting the purslane, wash and naturally dry it, put it in a far-infrared dryer below 50℃ and dry it for more than 24 hours, harvest 1 part by weight of purslane powder, wash and naturally dry it, and put it in a far-infrared dryer below 50℃ for 24 hours. 1 part by weight of perilla powder dried for more than an hour is mixed and extracted using a supercritical carbon dioxide extraction method. The extraction conditions are set at 60°C and 300 bar pressure with CO ₂ flow rate injected at 3 L/min. Afterwards, they are milked at 20.5°C for 15 minutes using a milking machine, the sediment is removed, and lignoceric acid is extracted. Afterwards, 3 parts by weight of the lignoceric acid was mixed with 97 parts by weight of perilla oil obtained by extracting the oil using a milking machine at 20°C for 15 minutes and removing the sediment to confirm the content of lignoceric acid.

[Example 5]

After harvesting the purslane, wash and naturally dry it, place it in a far-infrared dryer below 50℃ and dry it for more than 24 hours. Harvest 1 part by weight of purslane powder, wash and naturally dry it and place it in a far-infrared dryer below 50℃ for 24 hours. 1 part by weight of perilla powder dried for more than an hour is mixed and extracted using a supercritical carbon dioxide extraction method. The extraction conditions are set at 60°C and 400 bar pressure with CO ₂ flow rate injected at 3 L/min. Afterwards, they are milked at 20.5°C for 15 minutes using a milking machine, the sediment is removed, and the lignoceric acid is extracted. Afterwards, 3 parts by weight of the lignoceric acid was mixed with 97 parts by weight of perilla oil obtained by extracting the oil using a milking machine at 20°C for 15 minutes and removing the sediment to confirm the content of lignoceric acid.

[Example 6]

After harvesting the purslane, wash and naturally dry it, place it in a far-infrared dryer below 50℃ and dry it for more than 24 hours. Harvest 1 part by weight of purslane powder, wash and naturally dry it and place it in a far-infrared dryer below 50℃ for 24 hours. 1 part by weight of perilla powder dried for more than an hour is mixed and extracted using a supercritical carbon dioxide extraction method. The extraction conditions are set at 60°C and 500 bar pressure with CO ₂ flow rate injected at 3 L/min. Afterwards, they are milked at 20.5°C for 15 minutes using a milking machine, the sediment is removed, and the lignoceric acid is extracted. Afterwards, 3 parts by weight of the lignoceric acid was mixed with 97 parts by weight of perilla oil obtained by extracting the oil using a milking machine at 20°C for 15 minutes and removing the sediment to confirm the content of lignoceric acid.

[Comparative Example 6]

After harvesting the purslane, wash and naturally dry it, place it in a far-infrared dryer below 50℃ and dry it for more than 24 hours. Harvest 1 part by weight of purslane powder, wash and naturally dry it and place it in a far-infrared dryer below 50℃ for 24 hours. Mix 1 part by weight of perilla powder dried for more than an hour and extract them using a supercritical carbon dioxide extraction method. The extraction conditions are set at 60°C and 600 bar pressure with CO ₂ flow rate injected at 3 L/min. Afterwards, they are milked at 20.5°C for 15 minutes using a milking machine, the sediment is removed, and the lignoceric acid is extracted. Afterwards, 3 parts by weight of the lignoceric acid was mixed with 97 parts by weight of perilla oil obtained by extracting the oil using a milking machine at 20°C for 15 minutes and removing the sediment to confirm the content of lignoceric acid.

[Comparative Example 7]

After harvesting the purslane, wash and naturally dry it, place it in a far-infrared dryer below 50℃ and dry it for more than 24 hours. Harvest 1 part by weight of purslane powder, wash and naturally dry it and place it in a far-infrared dryer below 50℃ for 24 hours. 1 part by weight of perilla powder dried for more than an hour is mixed and extracted using a supercritical carbon dioxide extraction method. The extraction conditions are set at 70°C and 400 bar pressure with CO ₂ flow rate injected at 3 L/min. Afterwards, they are milked at 20.5°C for 15 minutes using a milking machine, the sediment is removed, and the lignoceric acid is extracted. Afterwards, 3 parts by weight of the lignoceric acid was mixed with 97 parts by weight of perilla oil obtained by extracting the oil using a milking machine at 20°C for 15 minutes and removing the sediment to confirm the content of lignoceric acid.

[Comparative Example 8]

After harvesting the purslane, wash and naturally dry it, place it in a far-infrared dryer below 50℃ and dry it for more than 24 hours. Harvest 1 part by weight of purslane powder, wash and naturally dry it and place it in a far-infrared dryer below 50℃ for 24 hours. Mix 1 part by weight of perilla powder dried for more than an hour and extract them using a supercritical carbon dioxide extraction method. The extraction conditions are set at 60°C and 400 bar pressure with CO ₂ flow rate injected at 2 L/min. Afterwards, they are milked at 20.5°C for 15 minutes using a milking machine, the sediment is removed, and the lignoceric acid is extracted. Afterwards, 3 parts by weight of the lignoceric acid was mixed with 97 parts by weight of perilla oil obtained by extracting the oil using a milking machine at 20°C for 15 minutes and removing the sediment to confirm the content of lignoceric acid.

[Comparative Example 9]

After harvesting the purslane, wash and naturally dry it, place it in a far-infrared dryer below 50℃ and dry it for more than 24 hours. Harvest 1 part by weight of purslane powder, wash and naturally dry it and place it in a far-infrared dryer below 50℃ for 24 hours. Mix 1 part by weight of perilla powder that has been dried for over an hour, and extract them using a supercritical carbon dioxide extraction method. The extraction conditions are set at 60°C and a pressure of 400 bar, with CO ₂ injected at a flow rate of 4 L/min. Afterwards, they are milked at 20.5°C for 15 minutes using a milking machine, the sediment is removed, and the lignoceric acid is extracted. Afterwards, 3 parts by weight of the lignoceric acid was mixed with 97 parts by weight of perilla oil obtained by extracting the oil using a milking machine at 20°C for 15 minutes and removing the sediment to confirm the content of lignoceric acid.

division pressure (bar) Temperature (℃) CO ₂ flow rate (L/min) Comparative Example 5 200 60 3 Example 4 300 60 3 Example 5 400 60 3 Example 6 500 60 3 Comparative Example 6 600 60 3 Comparative Example 7 400 70 3 Comparative Example 8 400 60 2 Comparative Example 9 400 60 4

division Lignoceric acid content (mg/g) Comparative Example 5 0.18 Example 4 0.33 Example 5 0.34 Example 6 0.30 Comparative Example 6 0.20 Comparative Example 7 0.25 Comparative Example 8 0.19 Comparative Example 9 0.25

As shown in Tables 5 and 6, it can be confirmed that the results of Examples 4 to 6 are good. This is believed to be a result of differences in pressure, temperature, and CO ₂ flow rate during supercritical carbon dioxide extraction.

First, in the case of Comparative Examples 5 and 8, it was determined that the supercritical carbon dioxide extraction conditions themselves were not conditions for sufficiently extracting lignoceric acid because the pressure was somewhat low or the CO ₂ flow rate was low.

In the case of Comparative Example 6, Comparative Example 7, and Comparative Example 9, it was determined that lignoceric acid was not sufficiently extracted due to problems such as destruction of nutrients due to high pressure, high temperature, or excessive CO ₂ flow rate.

Therefore, as in Examples 4 to 6, when using the supercritical carbon dioxide extraction method, in order to contain a high content of lignoceric acid, the CO ₂ flow rate was injected at 3 L/min, and the temperature was 60°C or lower and 300 to 500 bar. It is preferable to proceed with extraction under pressure. More specifically, it is desirable to inject CO ₂ at a flow rate of 3 L/min and perform extraction at a temperature of 60°C or lower and a pressure of 400 bar.

(D) Lignoceric acid content test according to milking

[Comparative Example 10]

After harvesting the purslane, wash and naturally dry it, place it in a far-infrared dryer below 50℃ and dry it for more than 24 hours. Harvest 1 part by weight of purslane powder, wash and naturally dry it and place it in a far-infrared dryer below 50℃ for 24 hours. 1 part by weight of perilla powder dried for more than an hour is mixed and extracted using a supercritical carbon dioxide extraction method. The extraction conditions are set at 60°C and 400 bar pressure with CO ₂ flow rate injected at 3 L/min. Afterwards, they are milked at 16.5°C for 15 minutes using a milking machine, the sediment is removed, and lignoceric acid is extracted. Afterwards, 3 parts by weight of the lignoceric acid was mixed with 97 parts by weight of perilla oil obtained by extracting the oil using a milking machine at 20°C for 15 minutes and removing the sediment to confirm the content of lignoceric acid.

[Example 7]

After harvesting the purslane, wash and naturally dry it, place it in a far-infrared dryer below 50℃ and dry it for more than 24 hours. Harvest 1 part by weight of purslane powder, wash and naturally dry it and place it in a far-infrared dryer below 50℃ for 24 hours. 1 part by weight of perilla powder dried for more than an hour is mixed and extracted using a supercritical carbon dioxide extraction method. The extraction conditions are set at 60°C and 400 bar pressure with CO ₂ flow rate injected at 3 L/min. Afterwards, they are milked at 18°C for 15 minutes using a milking machine, the sediment is removed, and the lignoceric acid is extracted. Afterwards, 3 parts by weight of the lignoceric acid was mixed with 97 parts by weight of perilla oil obtained by extracting the oil using a milking machine at 20°C for 15 minutes and removing the sediment to confirm the content of lignoceric acid.

[Example 8]

After harvesting the purslane, wash and naturally dry it, place it in a far-infrared dryer below 50℃ and dry it for more than 24 hours. Harvest 1 part by weight of purslane powder, wash and naturally dry it and place it in a far-infrared dryer below 50℃ for 24 hours. 1 part by weight of perilla powder dried for more than an hour is mixed and extracted using a supercritical carbon dioxide extraction method. The extraction conditions are set at 60°C and 400 bar pressure with CO ₂ flow rate injected at 3 L/min. Afterwards, they are milked at 20.5°C for 15 minutes using a milking machine, the sediment is removed, and the lignoceric acid is extracted. Afterwards, 3 parts by weight of the lignoceric acid was mixed with 97 parts by weight of perilla oil obtained by extracting the oil using a milking machine at 20°C for 15 minutes and removing the sediment to confirm the content of lignoceric acid.

[Example 9]

After harvesting the purslane, wash and naturally dry it, place it in a far-infrared dryer below 50℃ and dry it for more than 24 hours. Harvest 1 part by weight of purslane powder, wash and naturally dry it and place it in a far-infrared dryer below 50℃ for 24 hours. 1 part by weight of perilla powder dried for more than an hour is mixed and extracted using a supercritical carbon dioxide extraction method. The extraction conditions are set at 60°C and 400 bar pressure with CO ₂ flow rate injected at 3 L/min. Afterwards, they are milked at 23°C for 15 minutes using a milking machine, the sediment is removed, and the lignoceric acid is extracted. Afterwards, 3 parts by weight of the lignoceric acid was mixed with 97 parts by weight of perilla oil obtained by extracting the oil using a milking machine at 20°C for 15 minutes and removing the sediment to confirm the content of lignoceric acid.

[Comparative Example 11]

After harvesting the purslane, wash and naturally dry it, place it in a far-infrared dryer below 50℃ and dry it for more than 24 hours. Harvest 1 part by weight of purslane powder, wash and naturally dry it and place it in a far-infrared dryer below 50℃ for 24 hours. 1 part by weight of perilla powder dried for more than an hour is mixed and extracted using a supercritical carbon dioxide extraction method. The extraction conditions are set at 60°C and 400 bar pressure with CO ₂ flow rate injected at 3 L/min. Afterwards, they are milked at 25.5°C for 15 minutes using a milking machine, the sediment is removed, and the lignoceric acid is extracted. Afterwards, 3 parts by weight of the lignoceric acid was mixed with 97 parts by weight of perilla oil obtained by extracting the oil using a milking machine at 20°C for 15 minutes and removing the sediment to confirm the content of lignoceric acid.

division Milking temperature (℃) Comparative Example 10 16.5 Example 7 18.0 Example 8 20.5 Example 9 23.0 Comparative Example 11 25.5

division Lignoceric acid content (mg/g) Comparative Example 10 0.24 Example 7 0.33 Example 8 0.34 Example 9 0.32 Comparative Example 11 0.28

As shown in Tables 7 and 8, it can be confirmed that the results of Examples 7 to 9 are good. This is believed to be a result of the difference in milking temperature.

First, in the case of Comparative Example 10, it was determined that lignoceric acid was not sufficiently extracted due to low milking temperature and insufficient milking.

In the case of Comparative Example 11, it was determined that lignoceric acid was not sufficiently extracted due to problems such as destruction of nutrients due to high milking temperature.

Therefore, as in Examples 7 to 9, milking is preferably performed at 18 to 23°C for 15 minutes, and more specifically, is preferably performed at 20.5°C for 15 minutes.

As such, a person skilled in the art will understand that the technical configuration of the present invention described above can be implemented in other specific forms without changing the technical idea or essential features of the present invention.

Therefore, the embodiments described above should be understood in all respects as illustrative and not static, and the scope of the present invention is indicated by the claims described later rather than the detailed description above, and the meaning and scope of the claims and their Any changes or modifications derived from the equivalent concept should be construed as being included in the present invention.

S1: Mix of purslane powder and perilla seed powder
S2: Supercritical carbon dioxide extraction method extraction
S3: milking
S4: Sediment removal
S5: Lignoceric acid extraction

Claims (5)

The first step of mixing purslane powder and perilla seed powder;
A second step of producing a purslane and perilla mixed extract by extracting the mixed purslane powder and perilla seed powder using a supercritical carbon dioxide extraction method;
A third step of milking the purslane perilla mixed extract;
A fourth step of removing sediment from the extracted purslane and perilla mixed extract;
A lignoceric acid extraction method comprising a fifth step of extracting lignoceric acid from the purslane and perilla mixed extract from which the precipitate has been removed. According to claim 1,
The first step is,
A lignoceric acid extraction method characterized by mixing 1 part by weight of perilla seed powder with 1 part by weight of purslane powder. According to claim 1,
The second stage of extraction is,
A lignoceric acid extraction method that is carried out at a temperature of 60°C or lower and a pressure of 300 to 500 bar, and is injected at a CO ₂ flow rate of 3L/min. Using the lignoceric acid extracted according to item 1,
A method of manufacturing health food using a lignoceric acid extraction method, characterized in that it is produced by mixing the lignoceric acid and perilla oil. According to claim 4,
For 3 parts by weight of lignoceric acid,
A method of producing health food using a lignoceric acid extraction method, characterized in that 95 to 105 parts by weight of the perilla oil is mixed and used.