KR102055324B1 - Polyphenol-enriched plant extracts and producing method thereof - Google Patents
Polyphenol-enriched plant extracts and producing method thereof Download PDFInfo
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- KR102055324B1 KR102055324B1 KR1020180017553A KR20180017553A KR102055324B1 KR 102055324 B1 KR102055324 B1 KR 102055324B1 KR 1020180017553 A KR1020180017553 A KR 1020180017553A KR 20180017553 A KR20180017553 A KR 20180017553A KR 102055324 B1 KR102055324 B1 KR 102055324B1
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/10—General methods of cooking foods, e.g. by roasting or frying
- A23L5/15—General methods of cooking foods, e.g. by roasting or frying using wave energy, irradiation, electrical means or magnetic fields, e.g. oven cooking or roasting using radiant dry heat
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2250/00—Food ingredients
- A23V2250/20—Natural extracts
- A23V2250/21—Plant extracts
- A23V2250/2132—Other phenolic compounds, polyphenols
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2300/00—Processes
- A23V2300/14—Extraction
Abstract
과제: 폴리페놀 함량이 증가된 식물 추출물 및 그 제조방법을 제공하고, 폴리페놀 함량이 증가된 분말, 과립, 음료 등 다양한 형태의 식품을 제공하려는 것.
해결수단: 본 발명은 폴리페놀 함량이 증가된 식물 추출물 및 그 제조방법에 관한 것으로서, 좀 더 자세히는 식물을 식물성 기름을 이용하여 볶는 1차 볶음 공정, 수분을 첨가하여 볶는 2차 볶음 공정을 포함하는 폴리페놀 함량이 증가된 식물 추출물 및 그 제조방법에 관한 것이다. 본 발명의 폴리페놀 함량이 증가된 식물 추출물은 일반 건조 분말, 건조 볶음의 방법으로 추출된 식물 추출물과 비교할 때 폴리페놀 함량이 증가하였다. 또한, 본 발명의 폴리페놀 함량이 증가된 식물 추출물은 기름으로 볶는 공정으로 식물들의 비린 맛 또는 특유의 이취가 제거되어 고소한 맛으로 소비자들이 쉽게 섭취할 수 있다. 또한, 본 발명의 폴리페놀 함량이 증가된 식물 추출물은 유해한 용매를 포함하지 않아 인체에 안전하며, 다양한 식품 또는 약학 조성물에 효과적으로 적용할 수 있다.Challenge: To provide plant extracts with increased polyphenol content and methods for their preparation, and to provide various types of foods such as powders, granules and beverages with increased polyphenol content.
Solution: The present invention relates to a plant extract having an increased polyphenol content and a method for producing the same, and more particularly, a first roasting step of roasting a plant using vegetable oil and a second roasting step of adding water. The present invention relates to a plant extract having an increased polyphenol content and a preparation method thereof. The plant extract with increased polyphenol content of the present invention has an increased polyphenol content when compared to the plant extract extracted by the method of dry powder and dry roasting. In addition, the plant extract with increased polyphenol content of the present invention can be easily consumed by consumers in a savory taste by removing the fishy taste or unique off-flavor of the plants by the roasting process with oil. In addition, the plant extract with increased polyphenol content of the present invention does not contain harmful solvents and is safe for humans, and can be effectively applied to various food or pharmaceutical compositions.
Description
본 발명은 폴리페놀 함량이 증가한 식물 추출물 및 그 제조방법에 관한 것으로서, 좀 더 자세히는 식물을 식물성 기름을 이용하여 볶는 1차 볶음 공정, 수분을 첨가하여 볶는 2차 볶음 공정을 포함하는 폴리페놀 함량이 증가된 식물 추출물 및 그 제조방법에 관한 것이다.The present invention relates to a plant extract having an increased polyphenol content and a method of manufacturing the same, and more particularly, to a polyphenol content including a first roasting step of roasting a plant using vegetable oil and a second roasting step of adding water. The present invention relates to an increased plant extract and a preparation method thereof.
최근 현대인의 생활양식 변화로 인한 운동부족, 과로, 스트레스, 질병 및 체질 약화 등을 해소하기 위하여 기능성을 갖는 자연 건강식품에 대한 수요가 급격히 증가하고 있다. 식물로부터 기능성 성분을 추출하여 이 추출물을 식품 또는 의약품에 이용하는 것은 오래전부터 활발히 이루어지고 있다.Recently, the demand for natural health foods with functionalities is rapidly increasing in order to solve the lack of exercise, overwork, stress, disease and constitution caused by modern lifestyle changes. The extraction of functional ingredients from plants and the use of these extracts in foods or medicines have been active for a long time.
식물 추출 방법으로는 용매 추출, 감압 추출, 증기 추출, 압착 추출, 초음파 추출, 초임계 추출 등이 있다. 가장 일반적으로 사용되는 것은 물, 에틸아세테이트, 메탄올, 주정 등의 용매를 이용하여 추출하는 용매 추출법이다. 이 추출법은 용질에 따라 단일 용매 또는 비율을 조절한 혼합 용매를 사용한다. Plant extraction methods include solvent extraction, reduced pressure extraction, steam extraction, compression extraction, ultrasonic extraction, supercritical extraction. The most commonly used is a solvent extraction method using a solvent such as water, ethyl acetate, methanol, alcohol. This extraction method uses a single solvent or a mixed solvent in which the ratio is adjusted depending on the solute.
식물로부터 추출되는 기능성 성분은 추출 방법, 추출 용매에 따라 다양한 수율로 추출되며, 기능성 성분의 함량도 크게 달라진다. 따라서, 모든 성분을 추출하거나 원하는 성분만을 추출하거나 제거할 수 있는 용매 및 추출방법을 찾아 내는 것이 매우 중요하다.Functional components extracted from plants are extracted in various yields depending on the extraction method and the extraction solvent, the content of the functional components also vary greatly. Therefore, it is very important to find a solvent and an extraction method capable of extracting all components or extracting or removing only desired components.
폴리페놀은 식물이 자외선·활성산소·포식자로부터 스스로 보호하기 위해 만들어낸 일종의 방어물질을 통칭한다. 폴리페놀은 항산화 물질(Antioxidant)의 하나로서 인간의 건강유지와 노화방지, 질병 예방에 효과가 있다고 알려졌다. 항산화 물질은 몸 안의 활성산소를 해가 없는 물질로 바꿔 산화를 방지하는 물질이다. 활성산소는 체내 물질대사 과정에서 생성되는 산소 화합물이다. 반응성이 강해 DNA나 세포를 손상하고 그로 인해 노화 촉진이나 암, 동맥경화와 같은 질병을 유발하는 해로운 측면이 있다. 단, 적당히 있으면 세포의 성장과 분화를 도우며 세균이나 바이러스를 죽이는 역할을 하기도 한다. 프랑스인들이 육류 섭취가 많음에도 불구하고 심혈관 질환이 미국인의 3분의 1 수준이었던 이유는 프랑스인이 자주 마시는 레드 와인 속에 들어 있는 폴리페놀 성분 때문이라고 한다. Polyphenols are a type of defense that plants create to protect themselves from UV rays, free radicals, and predators. Polyphenols are known to be effective in maintaining human health, preventing aging and preventing diseases as one of antioxidants. Antioxidants prevent oxidation by converting free radicals into harmless substances. Free radicals are oxygen compounds that are produced during metabolism in the body. Its responsiveness damages DNA and cells, and has the harmful side effect of promoting aging and causing diseases such as cancer and arteriosclerosis. However, if moderately helps the growth and differentiation of cells, and also plays a role in killing bacteria or viruses. Despite the high intake of meat by the French, cardiovascular disease was one-third of Americans because of the polyphenols found in the red wines they often drink.
폴리페놀의 종류로는 카테킨(Catechin), 안토시아닌(Anthocyanin), 레스베라트롤(Resveratrol), 이소플라본(Isoflavone), 헤스페리딘(Hesperidin), 탄닌(Tannin), 커큐민(Curcumin) 등이 있다. Types of polyphenols include catechin, anthocyanin, resveratrol, isoflavone, hesperidin, tannin, and curcumin.
식물 추출방법에 대한 선행기술은 특허공개 제2018-0013140호 "식물 추출물의 제조방법"이 게재되어 있다. 이 선행기술은 식물과 제 1 수용성 용매를 혼합하여 제 1 수용성 추출물을 수득하는 제 1 단계; 상기 제 1 수용성 추출물과 식용 오일을 혼합하여 오일 추출물을 수득하는 제 2 단계; 상기 오일 추출물과 제 2 수용성 용매를 혼합하여 제 2 수용성 추출물을 수득하는 제 3 단계; 및 상기 제 2 수용성 추출물에서 제 2 수용성 용매를 제거하는 제 4 단계를 포함하는 식물 추출물의 제조방법에 대한 것으로서, 이 방법은 특정 성분의 제거 및/또는 기능성 물질의 농축을 위해 오일-물 분배계수 또는 옥탄올-물 분배계수의 차이를 이용하는 것으로서, 글리시리진을 감소시키는 효과를 거두었다. 그러나 이 방법은 실시예 1에 나타난 바와 같이, 에탄올 추출물을 얻는 단계; 오일을 가하여 오일층을 얻는 단계; 에탄올을 가하여 에탄올층을 얻는 단계; 에탄올을 다시 가하여 에탄올층을 얻는 단계; 및 에탄올을 다시 가하여 에탄올층을 얻는 단계; 진공농축단계;와 같이 여러 번의 추출단계를 거쳐야 하므로 시간 및 비용이 많이 소요되어 비경제적이다.Prior art for the method of extracting plants is published in Japanese Patent Publication No. 2018-0013140 "Method for producing a plant extract". This prior art comprises a first step of mixing a plant with a first water soluble solvent to obtain a first water soluble extract; A second step of obtaining an oil extract by mixing the first water soluble extract with an edible oil; A third step of mixing the oil extract with a second water soluble solvent to obtain a second water soluble extract; And a fourth step of removing the second water soluble solvent from the second water soluble extract, wherein the method comprises the oil-water partition coefficient for removal of specific components and / or concentration of functional substances. Or by using the difference of the octanol-water partition coefficient, which has the effect of reducing glycyrizine. However, this method comprises the steps of obtaining an ethanol extract, as shown in Example 1; Adding oil to obtain an oil layer; Adding ethanol to obtain an ethanol layer; Adding ethanol again to obtain an ethanol layer; And adding ethanol again to obtain an ethanol layer; It is uneconomical because it takes a lot of time and money because it has to go through several extraction steps, such as vacuum concentration step.
본 발명은 폴리페놀 함량이 증가한 식물 추출물을 제공하고, 이를 이용하여 폴리페놀 함량이 증가한 식물 추출물 분말, 과립, 음료 등 다양한 형태의 식품을 제공하려는 것을 목적으로 한다.An object of the present invention is to provide a plant extract with an increased polyphenol content, and to provide various types of foods such as plant extract powder, granules, beverages with an increased polyphenol content.
상기 목적을 달성하기 위하여 본 발명자는 식물을 식물성 기름을 이용하여 볶는 1차 볶음 공정 후, 수분을 첨가하여 2차 볶음 공정을 포함하여 제조하였다. 볶음 공정은 팬이나 넓은 용기를 가열한 후 기름을 넣고 식물을 넣어 익히거나 기름 대신 물과 같이 수분을 가하고 식물을 넣어 익히는 통상의 조리방법을 말한다.In order to achieve the above object, the present inventors have prepared a first roasting process of roasting plants using vegetable oil, and then adding water to prepare a second roasting process. The stir-frying process refers to a conventional cooking method in which a pan or a wide container is heated and then oil is added to cook the plants, or water is added instead of oil and water is cooked.
본 발명은 폴리페놀 함량이 증가된 식물 추출물 및 그 제조방법을 제공한다. The present invention provides a plant extract with an increased polyphenol content and a method for producing the same.
본 발명은The present invention
(가) 식물 100중량% 중, 식물 3~5중량%를 식물성 기름을 이용하여 볶는 1차 볶음 공정;(A) the primary roasting step of roasting 3 to 5% by weight of the plant using vegetable oil in 100% by weight of the plant;
(나) 상기 1차 볶음 공정 후 식물성 기름을 분리하는 공정;(B) separating vegetable oil after the first roasting step;
(다) 상기 식물성 기름 분리공정 후 식물성 기름에 용해된 지용성 유효성분을 추출하는 공정;(C) extracting the fat-soluble active ingredient dissolved in vegetable oil after the vegetable oil separation step;
(라) 상기 (나) 공정에서 식물성 기름 분리 후 남은 식물에 식물 95~97중량%를 더 넣고 총 식물 중량 100중량부에 대하여 수분 10~20중량부를 첨가하고 가열하여 볶는 2차 볶음 공정;(D) a second roasting step of adding 95 to 97% by weight of the plant to the remaining plant after separating the vegetable oil in step (B), adding 10 to 20 parts by weight of moisture to 100 parts by weight of the total plant weight, and roasting by heating;
(마) 상기 2차 볶음 공정 후 수분을 증발시키는 공정;(E) evaporating water after the second roasting step;
(바) 상기 수분 증발공정 후 잔여물을 발효하여 발효물을 얻는 공정; 및(F) fermenting the residue after the water evaporation step to obtain fermented products; And
(사) 상기 발효물 및 상기 추출된 지용성 유효성분을 혼합하여 식물 추출물을 얻는 공정;을 포함하는 폴리페놀 함량이 증가한 식물 추출물 제조방법에 관한 것이다.(G) a process for obtaining a plant extract by mixing the fermented product and the extracted fat-soluble active ingredient; and relates to a method for producing a plant extract with an increased polyphenol content.
삭제delete
또한, 본 발명은 상기 (사) 공정에서 얻은 식물 추출물이 액상, 반고체상, 과립상, 분말상 또는 환 형태임을 특징으로 하는 폴리페놀 함량이 증가한 식물 추출물 제조방법에 관한 것이다.In addition, the present invention relates to a method for producing a plant extract with increased polyphenol content, characterized in that the plant extract obtained in the step (g) is liquid, semi-solid, granular, powder or cyclic form.
또한, 본 발명은 상기 식물성 기름으로서 참기름, 들기름, 콩기름, 포도씨유, 해바라기씨유, 올리브유, 야자유, 코코넛유 중 선택되는 1종 이상을 사용하는 것을 특징으로 하는 폴리페놀 함량이 증가한 식물 추출물 제조방법에 관한 것이다.In addition, the present invention is a method for producing a plant extract with increased polyphenol content, characterized in that using at least one selected from sesame oil, perilla oil, soybean oil, grape seed oil, sunflower seed oil, olive oil, palm oil, coconut oil as the vegetable oil. It is about.
상기 방법으로 얻은 식물 추출물은 건강기능식품으로 섭취하거나, 음료 또는 조미용으로 이용할 수 있다.The plant extract obtained by the above method may be consumed as a dietary supplement, or used for beverage or seasoning.
본 발명의 폴리페놀 함량이 증가된 식물 추출물 및 그 제조방법의 추출대상이 되는 식물은 제한하지 않으며, 기능성 식물로 알려져 있거나, 기능성 물질을 함유하고 있을 것으로 예상되는 모든 종류의 식물이 추출 대상이 될 수 있다. The plant extract with increased polyphenol content of the present invention and the plant to be extracted are not limited, and all kinds of plants which are known as functional plants or are expected to contain a functional substance are to be extracted. Can be.
다래순은 목자·등리·미후리라고 불리며, 속명은 미후토이고, 산의 음지 계곡 주변에 분포되며, 개화시기는 5~6월이다. 다년목으로 잎은 호생 넓은 난형이며 가장자리에 가는 톱니가 있으며 덩굴 나무이다. 봄에 어린잎을 삶아서 말려두고 묵나물로 먹는다. 열매는 과일 또는 술로 담는다. 어린순은 4월 중순에서 5월 초순에 채취하며, 열매는 9월에 채취한다. 종류로는 다래·개다래·쥐다래가 있다. 여러가지 약리 작용을 하는데 열을 내리고 갈증을 멈추게 하며 이뇨작용도 있다. 만성 간염이나 간경화증으로 황달이 나타날 때, 구토나 소화불량에도 약효가 있으며, 비타민 C와 탄닌이 풍부해 피로를 풀어주고 불면증, 괴혈병에도 좋다. 다래는 비타민과 유기산, 당분, 단백질, 인, 나트륨, 칼륨, 마그네슘, 칼슘, 철분, 카로틴 등이 풍부하고, 특히 비타민 C가 풍부하여 항암 식물로 인정 받고 있다. 특히 위암을 예방하고 개선하는데 좋다.Daraesoon is called Shepherd, Deung-ri and Mihu-ri. Its name is Mi-futo, and it is distributed around the valley of valleys in the mountains. The flowering period is from May to June. It is a perennial tree and leaves are broad-leaved, ovate with fine teeth on the edges, and is a vine tree. Boil young leaves in spring, dry them, and eat them as mukmul. Fruits are served with fruit or alcohol. Young shoots are harvested from mid-April to early May, and fruit is harvested in September. There are varieties of keratin, dog antler, and rattan. There are various pharmacological actions that lower the fever and stop thirst and diuretic. When jaundice appears in chronic hepatitis or cirrhosis, it is effective in vomiting or indigestion. It is also rich in vitamin C and tannins, which are good for relieving fatigue and insomnia and scurvy. Tadasu is rich in vitamins, organic acids, sugars, proteins, phosphorus, sodium, potassium, magnesium, calcium, iron, carotene, etc., and especially rich in vitamin C, and is recognized as an anticancer plant. It is especially good for preventing and improving gastric cancer.
곤드레는 구멍이·도깨비엉겅퀴·고려가시나물·고려엉컹귀라고도 한다. 곤드레는 산과 들에서 자라며 높이 약 1m이며, 뿌리가 곧으며 가지가 사방으로 퍼진다. 곤드레 뿌리에 달린 입과 밑부분의 잎은 꽃이 필 때 시들며 줄기에 달린 잎은 타원 모양 바소꼴 또는 달걀 모양으로 밑쪽 잎은 잎자루가 길고 위쪽 잎은 잎자루가 짧은 특징이 있다. 잎의 앞면은 녹색에 털이 약간 나며, 뒷면은 흰색에 털이 없고 가장자리가 밋밋하거나 가시 같은 톱니가 있는 것이 특징이다. 곤드레는 7~10월에 지름 3~4cm의 붉은 자줏빛 관상화(管狀花)가 원줄기와 가지 끝에 한 송이씩 핀다. 총포는 둥근 종 모양으로 길이 약 2cm이고, 털이 빽빽하게 나며 화관은 자줏빛이고 길이 15~19mm이다. 열매는 수과로 길이 3.5~4mm의 킨 타원형이며 11월에 익으며 관모는 갈색이다. 곤드레는 어린 잎을 먹으며 한국 특산종으로 전국에 분포한다. 곤드레 나물은 혈액을 식혀주고 지혈 작용이 있으며, 어혈을 풀어주는 효과가 있다. 또한, 간세포의 기능을 활발하게 하는 기능이 있어서 간질환 즉 황달, 간경화로 복수가 찬 증상과 담석증을 치료하고, 이뇨 작용으로 부종이나 수종을 치료하는데 효과가 있다. 또한, 독극물, 인공 화학물질, 알콜 등을 중화 시키거나 해독하는 효능도 있다. Gondres are also known as perforations, goblin thistles, Korean herbaceous plants, and Korean burlesque. A gondre grows in mountains and fields, is about 1m high, and its roots are straight and its branches spread everywhere. The leaves on the mouth and bottom of the Gondre root wither when the flowers bloom. The leaves on the stem are oval-shaped or oval shaped, and the lower leaves have long petioles and the upper leaves have short petioles. The front side of the leaf is green with a little hair, and the back side is white with no hair and the edge is flat or thorny. Gondre blooms red and purple ornamentals, 3-4 cm in diameter, from July to October. The gun is round bell shaped, about 2cm long, dense hairs, corolla is purple, 15 ~ 19mm long. Fruits are achenes, long, 3.5 ~ 4mm long, oval, ripe in November, tubular, brown. Gondres eat young leaves and are native to Korea. Gondre herbs cool the blood, hemostatic action, and has the effect of releasing the blood. In addition, the function of activating the function of hepatocytes is effective in the treatment of hepatic disease, ie jaundice, hepatic cirrhosis, ascites and cholelithiasis, and diuretic edema or edema. It also has the effect of neutralizing or detoxifying poisons, artificial chemicals, alcohols, and the like.
삼지구엽초는 선령비(仙靈脾)·음양곽이라고도 한다. 뿌리줄기가 옆으로 꾸불꾸불 뻗으면서 잔뿌리가 많이 달린다. 줄기는 한 포기에서 여러 대가 뭉쳐나와 곧추 자라는데 가늘고 털이 없으며 밑 부분은 비늘 같은 잎으로 둘러싸여 있다. 줄기에서 가지가 3개로 갈라지고 그 가지 끝에 각각 3개씩 모두 9개의 잎이 달려 삼지구엽초라고 한다. 어릴 때는 잎이 꿩의다리·승마·깽깽이풀과 비슷하여 혼동하기 쉬우나 삼지구엽초의 잎은 심장 모양(하트)으로 생겼고 가장자리에 톱니처럼 패어들어간 자리가 있어 쉽게 구분할 수 있다. 관상용·약용으로 이용된다. 한방에서는 온포기를 음양곽이라 하여 약재로 쓴다. 잎과 줄기를 여름에 채취하여 술에 하룻밤 담갔다가 불에 말려 쓴다. 주침(酒浸)한 약술을 선령비주(仙靈脾酒) 또는 영패주(靈牌酒)라 한다. 약으로 쓸 때는 탕으로 하여 사용하며, 술을 담가서도 쓴다. 삼지구엽초는 주로 건강 생활을 돕고, 비뇨기·신경계 질환을 다스린다. 강장보호, 강정제, 갱년기장애, 건망증, 관절냉기, 근골위약, 냉병, 노망, 마비, 보신·보익, 불감증, 불임증, 빈뇨증, 사지동통, 설사, 야뇨증, 양기부족, 오로, 요슬산통, 우울증, 원기부족, 유정증, 음위, 이뇨, 자궁내막염, 자양강장, 저혈압, 정양, 중풍, 창종, 치조농루, 탈모증, 풍, 풍비, 풍습, 허랭에 효과가 있고, 소아질환으로 소아마비에도 효과가 있다고 알려져 있다.Samji-guyeokcho is also known as Seonryeongbi (仙靈 脾) and Eumyanggok. Root stem stretches sideways, and the roots run a lot. The stem grows upright by gathering several generations from one abandoned area. It is thin and hairless and the bottom is surrounded by scale-like leaves. Branches are divided into three branches on the stem, and each of the three leaves at the end of the branch is called trichophyte. When young, the leaves are similar to the pheasant's legs, horseback riding, and poultry grass, but the leaves of trilobites are shaped like a heart (heart), and the edges of the sawtooth are perforated so that they can be easily distinguished. It is used for ornamental and medicinal purposes. In Oriental medicine, Onpogi is used as a medicine called Yinyanggwa. Collect leaves and stems in summer, soak in alcohol overnight, and dry them on fire. Acupuncture medicines are called saengjubiju (仙靈 脾 酒) or Yeongapju (靈 牌 酒). When used as medicine, use it as a bath and soak alcohol. Trilobite vinegar mainly helps healthy life and manages urinary and nervous system diseases. Tonic protection, jeongjeongje, menopausal disorders, forgetfulness, joint cold, musculoskeletal, cold sickness, senility, paralysis, insured and injured, insensitivity, infertility, anuria, limb pain, diarrhea, nocturia, deprivation It is known to be effective in deficiency, wellbeing, vulgaris, diuresis, endometritis, nourishment tonic, hypotension, yangyang, para stroke, swelling, alveolar fever, alopecia, wind, abundance, customs, and squeeze. .
곰취는 웅소(熊蔬)라고도 한다. 곰취는 맛난 산나물로, 4월 말부터 6월 초순까지가 제철이다. 흔히 곰이 좋아한다고 해서 곰취라고 하는데, 무쳐 먹고 데쳐 먹고 쌈으로도 싸 먹을 수 있는 나물이라서 아주 인기가 좋다. 요즘은 강원도 인제와 태백 등지에서 인공재배도 많이 하는데, 떡도 해 먹고 말린 뒤 가루로 만들어 냉면에 섞어 먹기도 하며, 찐빵으로도 만들어 먹는다. 깊은 산에서 자생하는 여러해살이풀로, 산의 습한 지역에서 주로 자라며, 키는 1~2m이다. 잎은 심장형이며 길이는 약 30~35㎝, 폭은 40㎝가량 된다. 잎 가장자리에는 규칙적인 톱니가 나 있고 표면은 녹색이며 뒷면은 엷은 녹색을 띠고 있다. 꽃은 7~9월에 노란색으로 피는데, 지름은 약 4~5㎝ 정도이다. 잎 가운데 줄기에서 자주색을 띤 꽃대가 올라오고, 줄기에는 3~4장의 잎이 달려 있다. 10월경에 원통형 열매가 달리고, 종자에는 갈색 혹은 갈자색의 갓털이 있다. 웰빙 시대에 빠질 수 없는 식물 가운데 하나이지만 사람들이 너무 많이 채취하는 바람에 지금은 자생지 보호가 절실한 식물군 중의 하나가 되었다. 재배도 하지만 산에서 직접 채취하는 경우가 많기 때문이다. 약으로 쓸 때는 주로 탕으로 하여 사용한다. 주로 운동계의 통증과 순환계·호흡기 질환을 다스린다. 관절통, 담, 보신·보익, 양궐, 요통, 진정, 진통, 천식, 타박상, 해수, 행혈에 효과가 있고 소아질환으로 백일해에 효과가 있다. Bear tail is also called ungso (熊 蔬). The bear scent is a delicious wild vegetable, which is in season from late April to early June. It's often said that bears like bears, but they are very popular because they can be eaten, boiled, wrapped, and wrapped. Nowadays, there are also a lot of artificial cultivation in Inje and Taebaek, Gangwon-do, and after making rice cakes, they are dried and then made into powder and mixed with cold noodles. This is a perennial plant that grows in deep mountains. It grows mainly in the wet areas of the mountain and is 1 ~ 2m in height. The leaves are heart-shaped, about 30-35cm long, and 40cm wide. The leaf edge has regular sawtooth, the surface is green and the back is pale green. Flowers bloom yellow in July-September, about 4-5cm in diameter. A purple flowerbed comes up from the stem in the middle of the leaf, and the stem has three to four leaves. Cylindrical fruit runs around October, and seeds have brown or brown purple hairs. It is one of the indispensable plants in the well-being era, but it is now one of the plants in need of native habitat protection due to the excessive collection of people. It is also grown, but it is often harvested directly from the mountains. When used as medicine, mainly used as a bath. It primarily deals with pain in the motor system and circulatory and respiratory diseases. It is effective in joint pain, phlegm, bosin and bowel, arch, low back pain, soothing, analgesic, asthma, bruises, sea water, and bleeding.
본 발명의 식물 추출물 제조방법을 좀 더 자세히 설명한다. 먼저, 식물을 준비하여 세척하고 건조한다. 건조한 식물 100중량% 중, 식물 3~5중량%를 식물성 기름을 이용하여 볶는 1차 볶음 공정을 실시한다. 상기 1차 볶음 공정 후 기름을 분리하며, 분리된 기름에서 지용성 유효성분을 추출한다. 상기 기름을 분리하는 공정 후 볶은 식물에 식물 95~97중량%를 더 넣고 건조식물 총중량 100중량부 기준으로 수분 10~20중량부를 첨가하여 볶는 2차 볶음 공정을 실시한다. 상기 2차 볶음 공정 후 수분을 증발시킨다. 상기 수분을 증발시킨 잔여물을 발효하는 공정을 통해 발효물을 얻고, 이를 상기 지용성 유효성분과 혼합하여 폴리페놀 함량이 증가된 식물 추출물을 얻게 된다.The plant extract production method of the present invention will be described in more detail. First, the plants are prepared, washed and dried. In 100% by weight of dry plants, 3 to 5% by weight of plants using a vegetable oil to carry out the primary roasting step. The oil is separated after the first roasting process, and the fat-soluble active ingredient is extracted from the separated oil. After the process of separating the oil is added to 95 ~ 97% by weight of the plant to the roasted plants to add 10 to 20 parts by weight of water based on 100 parts by weight of the total weight of the dry plant to carry out the second roasting process. The water is evaporated after the second roasting process. A fermentation product is obtained through a process of fermenting the residue after evaporation of water, and the mixture is mixed with the fat-soluble active ingredient to obtain a plant extract having an increased polyphenol content.
상기 식물성 기름의 종류는 특별히 제한되지 않으나, 바람직하게는 참기름, 들기름, 콩기름, 해바라기씨유, 올리브유, 야자유, 코코넛유 중 선택되는 1종 이상인 것을 특징으로 하고, 더욱 바람직하게는 코코넛유를 사용할 수 있다. The type of vegetable oil is not particularly limited, but is preferably one or more selected from sesame oil, perilla oil, soybean oil, sunflower seed oil, olive oil, palm oil, coconut oil, more preferably coconut oil can be used. have.
상기 지용성 유효성분 추출 방법은 특별히 제한되지 않으나, 바람직하게는 2500 내지 3500 rpm으로 원심분리하는 것을 특징으로 하고, 3000 rpm으로 원심분리하는 것이 좀 더 바람직하다. 또는 지용성 유효성분 추출시 유기용제의 혼합물 또는 유기용제와 물의 혼합물을 가하여 혼합한 후 층 분리하여 추출하는 방법도 이용 가능하다.The fat-soluble effective ingredient extraction method is not particularly limited, but is preferably centrifuged at 2500 to 3500 rpm, more preferably centrifuged at 3000 rpm. Alternatively, a method of adding a mixture of an organic solvent or a mixture of an organic solvent and water when adding a fat-soluble effective ingredient and then mixing and extracting the layers may be used.
상기 잔여물을 발효하는 공정은 특별히 제한되지 않으나, 종균으로 순수 배양한 유산균 락토바실러스 플란타룸 (Lactobacillius plantarum)을 107cell/ml로 정제한 잔여물 내부에 직접 3%(w/w)를 접종하고 접종된 부위는 멸균 테이프로 봉합한 후, 온도 35℃ 조건에서 3일 동안 정치 배양하는 것을 특징으로 한다. 또한, 상기 발효된 잔여물을 파쇄 및 압착하여 즙으로 짜내어 10,000 × g에서 20분간 원심분리하여 침전물과 유산균체가 제거된 발효물을 얻는 것을 특징으로 한다. 잔여물 발효 공정은 위와 같이 순수 배양한 유산균 등을 이용하는 방법 외에 가정이나 농가에서 널리 사용하는 방법과 같이, 당을 가하여 배양하는 발효방법을 이용할 수도 있다. The process of fermenting the residue is not particularly limited, but 3% (w / w) directly into the residue purified from lactic acid bacterium Lactobacillius plantarum purely cultured to 10 7 cell / ml Inoculated and the inoculated site is sealed with sterile tape, it is characterized in that the stationary culture for 3 days at a temperature of 35 ℃. In addition, the fermented residue is crushed and squeezed with juice and centrifuged for 20 minutes at 10,000 × g, characterized in that the precipitate and lactic acid bacteria to remove the fermentation is obtained. The residue fermentation process may be a fermentation method in which sugar is added and cultured, such as a method widely used in homes or farms, in addition to a method of using lactic acid bacteria, which is purely cultured as described above.
본 발명으로 얻어진 폴리페놀 함량이 증가된 식물 추출물은 액상의 경우 바로 마실 수 있고, 냉수에 희석하여 차갑게 음료로 마시거나, 온수에 희석하여 따뜻한 차로 음용할 수 있다. 또한, 상기 액상의 식물 추출물은 건조하고 곱게 분쇄하여 분말 형태로 이용할 수도 있다. 또한, 상기 액상 식물 추출물과 상기 분말을 2:8 중량비로 교반기 또는 용기에 투입하여 균일하게 혼합하고 과립으로 제조하여 사용할 수 있고, 반고체상 또는 환의 형태로 제조할 수도 있다. Plant extracts with increased polyphenol content obtained by the present invention can be directly drinkable in the case of liquid, can be diluted with cold water to drink as a cold drink, or diluted with hot water to drink as a warm tea. In addition, the liquid plant extract may be dried and finely ground to be used in powder form. In addition, the liquid plant extract and the powder may be added to a stirrer or a container in a 2: 8 weight ratio to be uniformly mixed and used as granules, or may be prepared in the form of a semi-solid or cyclic form.
아울러, 상기 액상의 추출물, 분말, 과립, 반고체 또는 환은 건강기능식품, 건강보조식품, 일반 기호식품, 기능성 음료, 각종 음료수 등에 응용하여 사용할 수 있고, 예를 들어 캔디류, 초콜릿류, 젤리류, 스낵류, 과자류, 라면, 기타 면류, 아이스크림류, 껌류, 차, 드링크제, 음료수, 알콜 음료 등을 예로 들 수 있으나, 본 발명이 이에 제한되는 것은 아니다. In addition, the liquid extract, powder, granules, semi-solid or pill can be applied to health functional foods, health supplements, general taste foods, functional drinks, various beverages, and the like, for example, candy, chocolate, jelly, snacks , Confectionery, ramen, other noodles, ice cream, gum, tea, drinks, beverages, alcoholic beverages, etc., but the present invention is not limited thereto.
아울러, 상기 액상의 추출물은 파우치 형태로 밀봉 포장할 수도 있다. In addition, the liquid extract may be sealed packaging in the form of a pouch.
본 발명으로 얻어진 폴리페놀 함량이 증가된 식물 추출물은 각 원료의 영양학적 및 기능적 성분을 지속적으로 섭취할 수 있어 인체에 매우 유익하고 보관상 용이하고 운반이 쉬워 쉽고 간편하게 섭취할 수 있다. 또한, 소비자의 기호에 맞게 차로 마실 수 있으며, 우유, 요구르트 등에 첨가하여 음용하기에도 적당하다. 이뿐만 아니라, 각종 국, 반찬 등 요리에 감칠맛을 내게 하는 조미료로서 사용하는 것도 가능하다.Plant extracts with increased polyphenol content obtained by the present invention can be continuously ingested nutritional and functional components of each raw material is very beneficial to the human body, easy to store and easy to transport and can be easily consumed. In addition, it can be drunk as tea according to the consumer's preference, it is also suitable for drinking by adding milk, yogurt, and the like. Not only this, but also various seasonings, side dishes, etc. can be used as a seasoning to give a rich taste.
본 발명의 폴리페놀 함량이 증가된 식물 추출물은 일반 건조 분말, 건조 볶음의 방법으로 추출된 식물 추출물과 비교할 때 폴리페놀 함량이 증가하여 항산화 활성이 우수하다. The plant extract with increased polyphenol content of the present invention is excellent in antioxidant activity due to an increase in polyphenol content when compared to a plant extract extracted by a general dry powder or dry roasting method.
또한, 본 발명의 폴리페놀 함량이 증가된 식물 추출물은 기름으로 볶는 공정을 거치므로 지용성 성분 추출에 좋으며, 기름으로 볶은 후 물을 가하여 다시 추출하므로 일반 열수추출 또는 일반 오일 추출에서 추출하기 어려운 성분의 수율을 높이는 효과가 있을 것으로 예상된다. In addition, the plant extract with increased polyphenol content of the present invention is good for extracting fat-soluble components because it goes through the process of roasting with oil, it is extracted again by adding water after roasting with oil of the components that are difficult to extract in general hot water extraction or general oil extraction It is expected to increase the yield.
이뿐만 아니라, 본 발명의 추출방법은 식물들의 비린 맛 또는 특유의 이취를 제거하여 고소한 맛과 향취를 증대하여 소비자들이 쉽게 섭취할 수 있다.In addition, the extraction method of the present invention can be easily consumed by consumers by removing the fishy taste or peculiar odor of plants to increase the taste and flavor.
또한, 본 발명의 폴리페놀 함량이 증가된 식물 추출물은 유해한 용매를 포함하지 않아 인체에 안전하며, 다양한 식품 또는 약학 조성물에 효과적으로 응용할 수 있다.In addition, the plant extract with increased polyphenol content of the present invention does not contain harmful solvents and is safe for humans, and can be effectively applied to various food or pharmaceutical compositions.
또한, 본 발명의 폴리페놀 함량이 증가된 식물 추출물의 제조방법은 그 제조 공정이 간단하여 복잡하지 않은 설비 시설로 대량 생산이 가능하다. In addition, the method of producing a plant extract with increased polyphenol content of the present invention is a simple production process is possible to mass production in a complex facility.
이하, 본 발명의 구체적인 실시예를 들어 본 발명의 구성을 좀 더 자세히 설명한다. 그러나 본 발명의 범위는 실시예의 기재에만 한정되는 것이 아니며, 본 발명의 기술사상을 해하지 않는 범위 내에서 통상의 기술자가 변형 실시할 수 있으며, 이러한 변형 실시가 본 발명의 범위에 속함은 통상의 기술자에게 자명하다.Hereinafter, the configuration of the present invention will be described in more detail with reference to specific embodiments of the present invention. However, the scope of the present invention is not limited only to the description of the embodiments, it can be modified by those of ordinary skill in the art without departing from the technical spirit of the present invention, and such modifications are within the scope of the present invention. Self-explanatory
실시예Example 1: 폴리페놀 함량이 1: polyphenol content 증가된Increased 다래순Ascending 추출물 extract
건조한 다래순 100중량% 중 3~5중량%를 코코넛유를 이용하여 볶는 1차 볶음 공정을 실시하였다. 1차 볶음 공정 후 기름을 분리하고 기름에 용해되어있는 유효성분을 추출하였다. 식물의 지용성 유효성분을 추출한 후, 1차로 볶은 다래순에 나머지 다래순 95~97중량%를 넣고 다래순 총 건조중량 100중량부에 대하여 수분 10~20중량부를 첨가하고 가열하여 2차 볶음 공정을 실시하였다. 2차 볶음 공정 후 수분을 증발하였고, 잔여물에 설탕을 가하고 발효하여 발효물을 얻고, 상기 추출한 지용성 유효성분과 혼합하여 폴리페놀 함량이 증가된 식물 추출물을 제조하였다. The primary stir fry process was performed in which 3 to 5% by weight of 100% by weight of dried rattan sprouts were roasted using coconut oil. After the first roasting process, the oil was separated and the active ingredient dissolved in the oil was extracted. After extracting the fat-soluble active ingredient of the plant, add 95 to 97% by weight of the remaining taupe stalks to the first roasted taupe sprouts, add 10 to 20 parts by weight of water to 100 parts by weight of the total dry weight of the stalks and heat the second roasting process. Was carried out. Water was evaporated after the second roasting process, sugar was added to the residue, and the fermentation product was obtained by fermentation, and then mixed with the extracted fat-soluble active ingredient to prepare a plant extract having an increased polyphenol content.
실시예Example 2: 폴리페놀 함량이 2: polyphenol content 증가된Increased 곤드레 추출물 Gondre extract
건조한 곤드레 100중량% 중 3~5중량%를 코코넛유를 이용하여 볶는 1차 볶음 공정을 실시하였다. 1차 볶음 공정 후 기름을 분리하고, 기름에 용해되어 있는 유효성분을 추출하였다. 지용성 유효성분을 추출한 후, 1차로 볶은 곤드레에 나머지 곤드레 95~97중량%를 넣고 곤드레 총 건조중량 100중량부에 대하여 수분 10~20중량부를 첨가하고 가열하여 2차 볶음 공정을 실시하였다. 2차 볶음 공정 후 수분을 증발하였고, 증발 후 잔여물에 설탕을 가하고 발효하여 발효물을 얻고, 상기 추출한 지용성 유효성분과 혼합하여 폴리페놀 함량이 증가된 식물 추출물을 제조하였다. In the dry Gondre 100% by weight of 3 to 5% by weight of the first roasting process was carried out using coconut oil. After the first roasting process, the oil was separated and the active ingredient dissolved in the oil was extracted. After extracting the fat-soluble active ingredient, the remaining gondre was put in the first gondre 95 ~ 97% by weight, added 10 to 20 parts by weight of water with respect to 100 parts by weight of the total dry weight of the gondle was subjected to the second roasting process. After the second roasting process, water was evaporated, and after the evaporation, sugar was added to the residue, followed by fermentation to obtain fermented products, and mixed with the extracted fat-soluble active ingredient to prepare a plant extract having an increased polyphenol content.
실시예Example 3: 폴리페놀 함량이 3: polyphenol content 증가된Increased 삼지구엽초 추출물 Trilobite Leaf Extract
건조한 삼지구엽초 100중량% 중 3~5중량%를 코코넛유를 이용하여 볶는 1차 볶음 공정을 실시하였다. 1차 볶음 공정 후 기름을 분리하고, 기름에 용해되어 있는 유효성분을 추출하였다. 지용성 유효성분을 추출한 후, 1차로 볶은 삼지구엽초에 나머지 삼지구엽초 95~97중량%를 넣고 삼지구엽초 총 건조중량 100중량부에 대하여 수분 10~20중량부를 첨가하고 가열하여 2차 볶음 공정을 실시하였다. 2차 볶음 공정 후 수분을 증발하였고, 증발 후 잔여물에 설탕을 가하고 발효하여 발효물을 얻고, 상기 추출한 지용성 유효성분과 혼합하여 폴리페놀 함량이 증가된 식물 추출물을 제조하였다. A primary stir fry process was performed in which 3 to 5% by weight of 100% by weight of dried trilobite vinegar was roasted using coconut oil. After the first roasting process, the oil was separated and the active ingredient dissolved in the oil was extracted. After extracting the fat-soluble active ingredient, the remaining trigeminal vinegar 95-97% by weight was added to the first roasted trigeminal vinegar, 10-20 parts by weight of water was added to 100 parts by weight of the total dry weight of trigeminal vinegar, and the second roasting process was performed. . After the second roasting process, water was evaporated, and after the evaporation, sugar was added to the residue, followed by fermentation to obtain fermented products, and mixed with the extracted fat-soluble active ingredient to prepare a plant extract having an increased polyphenol content.
실시예Example 4: 폴리페놀 함량이 4: polyphenol content 증가된Increased 곰취 추출물 Bear Extract
건조한 곰취 100중량% 중 3~5중량%를 코코넛유를 이용하여 볶는 1차 볶음 공정을 실시하였다. 1차 볶음 공정 후 기름을 분리하고, 기름에 용해되어 있는 유효성분을 추출하였다. 지용성 유효성분을 추출한 후, 1차로 볶은 곰취에 나머지 곰취 95~97중량%를 넣고 곰취 총 건조중량 100중량부에 대하여 수분 10~20중량부를 첨가하고 가열하여 2차 볶음 공정을 실시하였다. 2차 볶음 공정 후 수분을 증발하였고, 증발 후 잔여물에 설탕을 가하고 발효하여 발효물을 얻고, 상기 추출한 지용성 유효성분과 혼합하여 폴리페놀 함량이 증가된 식물 추출물을 제조하였다. Three to five percent by weight of 100% by weight of dried bear odor was subjected to a first roasting process using roasted coconut oil. After the first roasting process, the oil was separated and the active ingredient dissolved in the oil was extracted. After extracting the fat-soluble active ingredient, the remaining Goji odor 95-97% by weight was added to the first roasted Goji odor, 10-20 parts by weight of water to 100 parts by weight of the total dry weight of Goji odor was carried out by a second roasting process. After the second roasting process, water was evaporated, and after the evaporation, sugar was added to the residue, followed by fermentation to obtain fermented products, and mixed with the extracted fat-soluble active ingredient to prepare a plant extract having an increased polyphenol content.
비교예Comparative example 1 : One : 다래순을Slap 이용한 추출물 (수분 첨가) Extract Used (Moisture Added)
건조한 다래순 100중량부에 수분 10~20중량부를 첨가하여 볶음 공정을 실시하고, 수분을 증발하고, 잔여물에 설탕을 가하고 발효하여 추출물을 얻었다.10 to 20 parts by weight of water was added to 100 parts by weight of dried rattan sprouts, followed by a roasting process, water was evaporated, sugar was added to the residue, followed by fermentation to obtain an extract.
비교예Comparative example 2 : 2 : 곤드레를Gondre 이용한 추출물 (수분 첨가) Extract Used (Moisture Added)
건조한 곤드레 100중량부에 수분 10~20중량부를 첨가하여 볶음 공정을 실시하고, 수분을 증발하고, 잔여물에 설탕을 가하고 발효하여 추출물을 얻었다. 10 to 20 parts by weight of water was added to 100 parts of dry gondre, followed by a roasting process, water was evaporated, sugar was added to the residue, followed by fermentation to obtain an extract.
비교예Comparative example 3 : 삼지구엽초를 이용한 추출물 (수분 첨가) 3: extract using trifoliar vinegar (moisture added)
건조한 삼지구엽초 100중량부에 수분 10~20중량부를 첨가하여 볶음 공정을 실시하고, 수분을 증발하고, 잔여물에 설탕을 가하고 발효하여 추출물을 얻었다.10 to 20 parts by weight of water was added to 100 parts by weight of dried trigeminal vinegar to carry out a roasting process, water was evaporated, sugar was added to the residue, and the resultant was fermented to obtain an extract.
비교예Comparative example 4 : 곰취를 이용한 추출물 (수분 첨가) 4: extract using bear odor (moisture added)
건조한 곰취 100중량부에 수분 10~20중량부를 첨가하여 볶음 공정을 실시하고, 수분을 증발하고, 잔여물에 설탕을 가하고 발효하여 추출물을 얻었다.10 to 20 parts by weight of moisture was added to 100 parts by weight of dried bear odor, followed by a roasting process. The water was evaporated, sugar was added to the residue, followed by fermentation to obtain an extract.
비교예Comparative example 5: 5: 다래순을Slap 이용한 추출물 (고온 볶음 + 수분 첨가) Extract Used (High Temperature Stirring + Water Added)
건조한 다래순 100중량부에 아무것도 첨가하지 않은 채 100℃ 이상으로 볶는 1차 볶음 공정을 실시하였다. 1차로 볶은 다래순에 다래순 건조중량기준으로 수분 10~20중량부를 첨가하여 2차 볶음 공정을 실시하였다. 2차 볶음 공정 후 수분을 증발시켰고, 잔여물에 설탕을 가하고 발효하여 추출물을 얻었다.A primary roasting step was performed to stir at 100 ° C. or more without adding anything to 100 parts by weight of dry rattan sprout. The second roasting process was carried out by adding 10 to 20 parts by weight of moisture on the basis of dry weight on the basis of the first roasted rattan. Water was evaporated after the second roasting process, and sugar was added to the residue, followed by fermentation to obtain an extract.
비교예Comparative example 6: 6: 곤드레를Gondre 이용한 추출물 (고온 볶음 + 수분 첨가) Extract Used (High Temperature Stirring + Water Added)
건조한 곤드레 100중량부에 아무것도 첨가하지 않은 채 100℃ 이상으로 볶는 1차 볶음 공정을 실시하였다. 1차로 볶은 곤드레에 곤드레 건조중량 기준으로 수분 10~20중량부를 첨가하여 2차 볶음 공정을 실시하였다. 2차 볶음 공정 후 수분을 증발시키고, 잔여물에 설탕을 가하고 발효하여 추출물을 얻었다. The primary roasting process was performed to stir at 100 ° C. or more without adding anything to 100 parts by weight of dry gondre. 10 to 20 parts by weight of moisture was added to the gondre roasted first, based on the dry weight of the gondle, and the second roasting process was performed. Water was evaporated after the second roasting process, sugar was added to the residue and the mixture was fermented to obtain an extract.
비교예Comparative example 7: 삼지구엽초를 이용한 추출물 (고온 볶음 + 수분 첨가) 7: Extract using Trifolium vinegar (hot stir + water added)
건조한 삼지구엽초 100중량부에 아무것도 첨가하지 않은 채 100℃ 이상으로 볶는 1차 볶음 공정을 실시하였다. 1차로 볶은 삼지구엽초에 삼지구엽초 건조중량 기준으로 수분 10~20중량부를 첨가하여 2차 볶음 공정을 실시하였다. 2차 볶음 공정 후 수분을 증발시키고, 잔여물에 설탕을 가하고 발효하여 추출물을 얻었다. The primary roasting process was carried out to stir at 100 ° C or more without adding anything to 100 parts by weight of dry trigeminal vinegar. The first roasted trigeminal vinegar was added to 10-20 parts by weight of moisture based on the dry weight of trigeminal vinegar to carry out the second roasting process. Water was evaporated after the second roasting process, sugar was added to the residue, followed by fermentation to obtain an extract.
비교예Comparative example 8: 곰취를 이용한 추출물 (고온 볶음 + 수분 첨가) 8: Extract using bear odor (hot fry + water added)
건조한 곰취 100중량부에 아무것도 첨가하지 않은 채 100℃ 이상으로 볶는 1차 볶음 공정을 실시하였다. 1차로 볶은 곰취에 곰취 건조중량 기준으로 수분 10~20중량부를 첨가하여 2차 볶음 공정을 실시하였다. 2차 볶음 공정 후 수분을 증발시키고 잔여물에 설탕을 가하고 발효하여 추출물을 얻었다. The primary roasting process was carried out to stir at 100 ° C or more without adding anything to 100 parts by weight of dry bear odor. The first roasted bear odor was added to 10 to 20 parts by weight based on the dry weight of the bear odor to carry out the second roasting process. Water was evaporated after the second roasting process, sugar was added to the residue, followed by fermentation to obtain an extract.
실험예Experimental Example 1: 폴리페놀 함량 측정 1: Polyphenol Content Determination
실시예 1~4의 폴리페놀 함량이 증가된 식물 추출물 및 비교예 1~8의 추출물의 폴리페놀 함량을 측정하였다. 측정 결과는 표 1과 같다. The polyphenol contents of the plant extracts having increased polyphenol contents of Examples 1 to 4 and the extracts of Comparative Examples 1 to 8 were measured. The measurement results are shown in Table 1.
(mg/100g)Polyphenols
(mg / 100g)
(mg/100g)Polyphenols
(mg / 100g)
(mg/100g)Polyphenols
(mg / 100g)
상기 표 1에서 보는 바와 같이, 본 발명의 제조방법으로 제조된 실시예 1의 다래순 추출물은 건조 다래순을 추출한 비교예 1 및 건조 다래순을 열에 볶아서 추출한 비교예 5와 비교하였을 때 폴리페놀 함량이 증가한 것을 알 수 있었다. 다래순뿐만 아니라, 실시예 2의 곤드레 추출물 또한, 건조 곤드레를 추출한 비교예 2와 건조 곤드레를 열에 볶아서 추출한 비교예 6과 비교하였을 때, 폴리페놀 함량이 증가하였다. 또한, 실시예 3의 삼지구엽초 추출물도 삼지구엽초를 추출한 비교예 3과 건조 삼지구엽초를 열에 볶아서 추출한 비교예 7과 비교하였을 때, 폴리페놀 함량이 월등히 증가함을 알 수 있었다. 또한, 실시예 4의 곰취 추출물도 건조 곰취를 추출한 비교예 4와 건조 곰취를 열에 볶아서 추출한 비교예 8과 비교하였을 때, 폴리페놀 함량이 월등히 증가함을 알 수 있었다.As shown in Table 1, the pleural sprout extract of Example 1 prepared by the manufacturing method of the present invention is polyphenol content when compared with Comparative Example 1 extracted dry snail order and Comparative Example 5 extracted by roasting dried snail order This increased. In addition to the order of the order, the Gondre extract of Example 2 also increased the polyphenol content when compared with Comparative Example 2, which was extracted with dried Gondre, and Comparative Example 6, which was obtained by roasting dry Gondre with heat. In addition, it can be seen that the polyphenol content was significantly increased when comparing the Trigeminal vinegar extract of Example 3 with Comparative Example 3, which was extracted with Trigeminal vinegar, and Comparative Example 7, which was extracted by roasting dried Trigeminal vinegar with heat. In addition, it was found that the polyphenol content was significantly increased as compared with Comparative Example 4, which extracts dried Bear odor and Comparative Example 8, which was extracted by roasting dried Bear odor in heat.
상기 결과로부터 본 발명의 방법에 의해 제조된 식물 추출물은 폴리페놀 함량이 증가하였음을 알 수 있다. It can be seen from the above results that the plant extract prepared by the method of the present invention has an increased polyphenol content.
실험예Experimental Example 2: 2: 관능 검사Sensory evaluation 1 ( One ( 실시예Example 1, One, 비교예Comparative example 1, One, 비교예Comparative example 5) 5)
실시예 1의 폴리페놀 함량이 증가된 다래순 추출물, 비교예 1 및 비교예 5에 대하여 20~60대 일반 성인 남녀 각각 20명을 선발하여 향미, 맛, 복용 용이성 및 종합 기호도에 대하여 관능검사를 실시하여 표 2에 나타내었다. Twenty female adults of 20 to 60 years of age were selected for the extract of Rassau L. extract, Comparative Example 1 and Comparative Example 5, which were increased in Example 1, and the sensory tests were conducted on flavor, taste, ease of taking, and overall preference. It carried out and is shown in Table 2.
(1: 매우 나쁨, 2: 나쁨, 3: 약간 나쁨, 4: 보통, 5: 약간 좋음, 6: 좋음, 7: 아주 좋음)(1: Very bad, 2: Bad, 3: Slightly bad, 4: Normal, 5: Slightly good, 6: Good, 7: Very good)
실험예Experimental Example 3: 3: 관능 검사Sensory evaluation 2 ( 2 ( 실시예Example 2, 2, 비교예Comparative example 2, 2, 비교예Comparative example 6) 6)
실시예 2의 폴리페놀 함량이 증가된 곤드레 추출물, 비교예 2및 비교예 6에 대하여 20~60대 일반 성인 남녀 각각 20명을 선발하여 향미, 맛, 복용 용이성 및 종합 기호도에 대하여 관능검사를 실시하여 표 3에 나타내었다. In the Gondre extract with increased polyphenol content of Example 2, Comparative Example 2 and Comparative Example 6, 20 general adult men and women in their 20's and 60's were selected, and sensory tests were conducted on flavor, taste, ease of taking, and overall preference. It is shown in Table 3.
(1: 매우 나쁨, 2: 나쁨, 3: 약간 나쁨, 4: 보통, 5: 약간 좋음, 6: 좋음, 7: 아주 좋음)(1: Very bad, 2: Bad, 3: Slightly bad, 4: Normal, 5: Slightly good, 6: Good, 7: Very good)
실험예Experimental Example 4: 4: 관능 검사Sensory evaluation 3 ( 3 ( 실시예Example 3, 3, 비교예Comparative example 3, 3, 비교예Comparative example 7) 7)
실시예 3의 폴리페놀 함량이 증가된 삼지구엽초 추출물, 비교예 3 및 비교예 7에 대하여 20~60대 일반 성인 남녀 각각 20명을 선발하여 향미, 맛, 복용 용이성 및 종합 기호도에 대하여 관능검사를 실시하여 표 4에 나타내었다. Twenty female adults and twenties in their 20's and 60's were selected for the trifoliate vinegar extract, Comparative Example 3 and Comparative Example 7, which increased the polyphenol content of Example 3, and the sensory test was conducted for flavor, taste, ease of taking, and overall preference. It carried out and is shown in Table 4.
(1: 매우 나쁨, 2: 나쁨, 3: 약간 나쁨, 4: 보통, 5: 약간 좋음, 6: 좋음, 7: 아주 좋음)(1: Very bad, 2: Bad, 3: Slightly bad, 4: Normal, 5: Slightly good, 6: Good, 7: Very good)
실험예Experimental Example 5: 5: 관능 검사Sensory evaluation 4 ( 4 ( 실시예Example 4, 4, 비교예Comparative example 4, 4, 비교예Comparative example 8) 8)
실시예 4의 폴리페놀 함량이 증가된 곰취 추출물, 비교예 4 및 비교예 8에 대하여 20~60대 일반 성인 남녀 각각 20명을 선발하여 향미, 맛, 복용 용이성 및 종합 기호도에 대하여 관능검사를 실시하여 표 5에 나타내었다.20 adult males and females in their 20's and 60's were selected for the extract of Bear extract with increased polyphenol content of Example 4, Comparative Examples 4 and 8, and sensory tests were conducted for flavor, taste, ease of taking, and general preference. It is shown in Table 5.
(1: 매우 나쁨, 2: 나쁨, 3: 약간 나쁨, 4: 보통, 5: 약간 좋음, 6: 좋음, 7: 아주 좋음)(1: Very bad, 2: Bad, 3: Slightly bad, 4: Normal, 5: Slightly good, 6: Good, 7: Very good)
본 발명의 실시예 1~4의 폴리페놀 함량이 증가된 식물 추출물과 비교예 1~8의 추출물에 대해, 향미, 맛, 복용 용이성 및 종합 기호도를 평가한 결과, 본 발명의 실시예 1~4의 폴리페놀 함량이 증가된 식물 추출물이 향미, 맛, 복용 용이성, 종합 기호도에서 월등히 높은 평가를 받았다. 본 발명은 기름으로 볶는 공정을 포함하여 식물의 비린 맛 또는 특유의 이취가 제거되었고, 고소한 맛을 더하여 소비자들이 쉽게 음용할 수 있게 되었다.As a result of evaluating flavor, taste, ease of taking, and overall preference of the plant extract with increased polyphenol content of Examples 1 to 4 of the present invention and the extracts of Comparative Examples 1 to 8, Examples 1 to 4 of the present invention Plant extracts with increased polyphenol content were highly rated in flavor, taste, ease of use and general preference. The present invention removes the fishy taste or characteristic off-flavor of the plant, including the process of roasting with oil, and can be easily consumed by consumers by adding a savory taste.
Claims (5)
(나) 상기 1차 볶음 공정 후 식물성 기름을 분리하는 공정;
(다) 상기 식물성 기름 분리공정 후 식물성 기름에 용해된 지용성 유효성분을 추출하는 공정;
(라) 상기 (나) 공정에서 식물성 기름 분리 후 남은 식물에 식물 95~97중량%를 더 넣고 총 식물 중량 100중량부에 대하여 수분 10~20중량부를 첨가하고 가열하여 볶는 2차 볶음 공정;
(마) 상기 2차 볶음 공정 후 수분을 증발시키는 공정;
(바) 상기 수분 증발공정 후 잔여물에 유산균 또는 당을 가하고 배양하여 발효물을 얻는 공정; 및
(사) 상기 발효물 및 상기 추출된 지용성 유효성분을 혼합하여 식물 추출물을 얻는 공정;을 포함하는 폴리페놀 함량이 증가한 식물 추출물 제조방법.
(A) the primary roasting step of roasting 3 to 5% by weight of the plant using vegetable oil in 100% by weight of the polyphenol-containing plant;
(B) separating vegetable oil after the first roasting step;
(C) extracting the fat-soluble active ingredient dissolved in vegetable oil after the vegetable oil separation step;
(D) a second roasting step of adding 95 to 97% by weight of plants to the remaining plant after separation of vegetable oil in step (b), adding 10 to 20 parts by weight of water to 100 parts by weight of the total plant weight, and heating and roasting;
(E) evaporating water after the second roasting step;
(F) adding lactic acid bacteria or sugar to the residue after the water evaporation step to obtain a fermented product; And
(G) a process for obtaining a plant extract by mixing the fermented product and the extracted fat-soluble active ingredient; plant extract manufacturing method with increased polyphenol content comprising a.
상기 (사) 공정에서 얻은 식물 추출물은 액상, 반고체상, 과립상, 분말상 또는 환 형태임을 특징으로 하는 폴리페놀 함량이 증가한 식물 추출물 제조방법.
The method according to claim 1,
Plant extract obtained in the step (g) is a method of producing a plant extract with increased polyphenol content, characterized in that the liquid, semi-solid, granular, powder or cyclic form.
상기 식물성 기름은 참기름, 들기름, 콩기름, 포도씨유, 해바라기씨유, 올리브유, 야자유, 코코넛유 중 선택되는 1종 이상인 것을 특징으로 하는 폴리페놀 함량이 증가한 식물 추출물 제조방법.
The method according to claim 1,
The vegetable oil is a method of producing a plant extract with increased polyphenol content, characterized in that at least one selected from sesame oil, perilla oil, soybean oil, grape seed oil, sunflower seed oil, olive oil, palm oil, coconut oil.
상기 식물 추출물은 음료 또는 조미용으로 이용함을 특징으로 하는 폴리페놀 함량이 증가한 식물 추출물 제조방법.The method according to claim 1,
The plant extract is a method for producing a plant extract with increased polyphenol content, characterized in that it is used for beverages or seasonings.
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