KR20230134035A - Composition for antioxidant or antiobesity comprising enzyme treated fruit and method for manufacturing the same - Google Patents
Composition for antioxidant or antiobesity comprising enzyme treated fruit and method for manufacturing the same Download PDFInfo
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- KR20230134035A KR20230134035A KR1020220030496A KR20220030496A KR20230134035A KR 20230134035 A KR20230134035 A KR 20230134035A KR 1020220030496 A KR1020220030496 A KR 1020220030496A KR 20220030496 A KR20220030496 A KR 20220030496A KR 20230134035 A KR20230134035 A KR 20230134035A
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- fruit
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
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- A—HUMAN NECESSITIES
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- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
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- A61K8/9783—Angiosperms [Magnoliophyta]
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- A—HUMAN NECESSITIES
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A61K2236/10—Preparation or pretreatment of starting material
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Abstract
본 발명은 효소 처리된 과일을 포함하는 항비만용 또는 항산화용 조성물과, 이의 제조 방법에 관한 것이다. 본 발명의 일 측면인 효소 처리된 과일을 포함하는 조성물은 클로로겐산과 같은 페놀류 등을 과량 함유하고 있어, 항산화 효과가 우수하다. 또한, 본 발명에 따른 조성물은 환원당 함량이 높고, 투명도 및 색도가 높아 기호성이 우수하다. 또한, 본 발명에 따른 조성물은 지방세포 분화 억제능이 우수하여 항비만· 체중 감량 효과가 우수하다. 또한, 본 발명의 일 측면인 효소 처리 과일 조성물의 제조 방법은 과일로부터 생리활성성분을 다량 효과적으로 추출할 수 있어 경제적이다.The present invention relates to an anti-obesity or antioxidant composition containing enzyme-treated fruits and a method for producing the same. The composition containing enzyme-treated fruit, which is one aspect of the present invention, contains an excessive amount of phenols such as chlorogenic acid, and has excellent antioxidant effect. In addition, the composition according to the present invention has a high reducing sugar content and excellent palatability due to high transparency and chromaticity. In addition, the composition according to the present invention has excellent anti-obesity and weight loss effects due to its excellent ability to inhibit adipocyte differentiation. In addition, the method for producing an enzyme-treated fruit composition, which is an aspect of the present invention, is economical because it can effectively extract a large amount of bioactive ingredients from fruit.
Description
본 발명은 효소 처리된 과일을 포함하는 항비만용 또는 항산화용 조성물과, 이의 제조 방법에 관한 것이다. The present invention relates to an anti-obesity or antioxidant composition containing enzyme-treated fruits and a method for producing the same.
과일은 각종 비타민, 미네랄, 유기산, 아미노산 등의 생리활성성분을 풍부하게 함유하고 있어, 최고의 자연 건강식품으로 알려져 있다. Fruits are known to be the best natural health food as they are rich in bioactive ingredients such as various vitamins, minerals, organic acids, and amino acids.
그러나, 최근들어 과일에 포함된 당이 비만 등의 성인병을 유발할 수 있다는 인식이 강해지면서, 일반인들조차도 과일의 섭취를 꺼려하는 성향이 짙어지고 있다.However, recently, as awareness has grown that the sugar contained in fruits can cause adult diseases such as obesity, even the general public has become increasingly reluctant to consume fruits.
또한, 과일은 개체간의 맛, 당도 등의 품질 차이가 크고, 저장성이 좋지 않아서, 주로, 주스 잼, 소스, 차(tea) 등으로 가공되어 유통되는 경우가 많다. 이러한 가공식품들은 가공과정 중에 과일에 함유된 생리활성성분들이 파괴되고, 첨가된 인공 감미료가 인체에 해로운 영향을 끼친다는 문제가 지적되고 있다. In addition, fruits have large differences in quality such as taste and sweetness between individuals, and have poor storage properties, so they are often processed and distributed into juice, jam, sauce, tea, etc. The problem with these processed foods is that the biologically active ingredients contained in the fruit are destroyed during the processing process, and the added artificial sweeteners have a harmful effect on the human body.
이에, 과일에 포함된 생리활성성분을 온전히 소비자에게 전달할 수 있으면서, 성인병 유발 이슈로부터 자유로운 과일 가공법에 대한 연구가 활발히 진행되고 있으나, 아직까지 만족할 만한 결과를 얻은 결과는 보고된 바 없다.Accordingly, research is being actively conducted on fruit processing methods that can fully deliver the bioactive ingredients contained in fruits to consumers and are free from issues causing adult diseases, but no satisfactory results have been reported yet.
일 측면에서 본 발명의 목적은 인체에 무해하며, 맛·외관 등의 기호성이 향상된 과일 가공물을 제공하는 것이다.In one aspect, the purpose of the present invention is to provide processed fruit products that are harmless to the human body and have improved palatability such as taste and appearance.
일 측면에서 본 발명의 목적은 항산화 효과가 우수한, 과일 함유 조성물을 제공하는 것이다.In one aspect, an object of the present invention is to provide a fruit-containing composition with excellent antioxidant effect.
일 측면에서 본 발명의 목적은 지방세포 분화 억제능이 우수한, 과일 함유 조성물을 제공하는 것이다.In one aspect, an object of the present invention is to provide a fruit-containing composition that has excellent ability to inhibit adipocyte differentiation.
일 측면에서 본 발명의 목적은 비만 예방 및 개선 효과가 우수한, 과일 함유 조성물을 제공하는 것이다.In one aspect, the purpose of the present invention is to provide a fruit-containing composition that is excellent in preventing and improving obesity.
일 측면에서 본 발명의 목적은 저장성이 우수한 과일 추출물을 제공하는 것이다.In one aspect, the purpose of the present invention is to provide a fruit extract with excellent storage properties.
일 측면에서 본 발명의 목적은 과일에 함유된 생리활성물질을 효과적으로 추출하는 방법을 제공하는 것이다.In one aspect, the purpose of the present invention is to provide a method for effectively extracting bioactive substances contained in fruits.
일 측면에서 본 발명의 목적은 추출수율이 우수한 과일 추출법을 제공하는 것이다.In one aspect, the purpose of the present invention is to provide a fruit extraction method with excellent extraction yield.
일 측면에서 본 발명의 목적은 클로로젠산 추출효율이 우수한 과일 추출법을 제공하는 것이다.In one aspect, the purpose of the present invention is to provide a fruit extraction method with excellent chlorogenic acid extraction efficiency.
상기 목적을 달성하기 위하여, 본 발명은 일 측면에서 효소 처리된 과일을 포함하는 조성물로서, 상기 효소는 셀루클라스트(Celluclast) 및 비스코자임(Viscozyme)을 포함하며, 상기 비스코자임은, 상기 셀루클라스트를 100 중량부로할 때, 0.1~10 중량부로 포함되는, 효소 처리된 과일을 포함하는 조성물을 제공한다.In order to achieve the above object, the present invention is, in one aspect, a composition comprising enzyme-treated fruit, wherein the enzyme includes Celluclast and Viscozyme, and the Viscozyme includes the Celluclast and Viscozyme. A composition comprising enzyme-treated fruit is provided in an amount of 0.1 to 10 parts by weight based on 100 parts by weight of fruit.
상기 목적을 달성하기 위하여, 본 발명은 일 측면에서 상기 효소 처리된 과일을 포함하는 조성물은, 비스코자임을 처리한 후 셀루클라스트를 처리한 과일을 포함하는, 효소 처리된 과일을 포함하는 조성물을 제공한다.In order to achieve the above object, the present invention, in one aspect, is a composition containing the enzyme-treated fruit, which includes fruit treated with viscozyme and then treated with celluclast. to provide.
상기 목적을 달성하기 위하여, 본 발명은 일 측면에서 상기 과일은 사과를 포함하는, 효소 처리된 과일을 포함하는 조성물을 제공한다.In order to achieve the above object, the present invention provides a composition comprising enzyme-treated fruits, in one aspect the fruits include apples.
상기 목적을 달성하기 위하여, 본 발명은 일 측면에서 상기 효소 처리된 과일을 포함하는 조성물은, 항산화용 조성물을 포함하는, 효소 처리된 과일을 포함하는 조성물을 제공한다.In order to achieve the above object, the present invention provides, in one aspect, a composition containing enzyme-treated fruit, wherein the composition containing the enzyme-treated fruit includes an antioxidant composition.
상기 목적을 달성하기 위하여, 본 발명은 일 측면에서 상기 효소 처리된 과일을 포함하는 조성물은, 비만 예방용 또는 개선용 조성물을 포함하는, 효소 처리된 과일을 포함하는 조성물을 제공한다.In order to achieve the above object, the present invention provides, in one aspect, a composition containing enzyme-treated fruit, wherein the composition containing the enzyme-treated fruit includes a composition for preventing or improving obesity.
상기 목적을 달성하기 위하여, 본 발명은 일 측면에서 상기 효소 처리된 과일을 포함하는 조성물은, 식품 조성물, 약학적 조성물 또는 화장료 조성물을 포함하는, 효소 처리된 과일을 포함하는 조성물을 제공한다.In order to achieve the above object, the present invention provides, in one aspect, a composition containing enzyme-treated fruit, wherein the composition containing the enzyme-treated fruit includes a food composition, pharmaceutical composition, or cosmetic composition.
상기 목적을 달성하기 위하여, 본 발명은 일 측면에서 효소 처리된 과일을 포함하는 조성물의 제조 방법으로서, 상기 방법은, 과일에 셀루클라스트(Celluclast) 및 비스코자임(Viscozyme)을 처리하는 단계를 포함하는, 효소 처리된 과일을 포함하는 조성물의 제조 방법을 제공한다.In order to achieve the above object, the present invention is, in one aspect, a method for producing a composition containing enzyme-treated fruit, the method comprising the step of treating the fruit with Celluclast and Viscozyme. Provided is a method for producing a composition comprising enzyme-treated fruit.
상기 목적을 달성하기 위하여, 본 발명은 일 측면에서 상기 방법은, 과일에 비스코자임을 1~10시간 동안 처리한 후, 비스코자임을 처리한 과일에 셀루클라스트를 0.5~2시간 동안 처리하는 것을 포함하는, 효소 처리된 과일을 포함하는 조성물의 제조 방법을 제공한다.In order to achieve the above object, the present invention in one aspect includes treating the fruit with Viscozyme for 1 to 10 hours and then treating the Viscozyme-treated fruit with Celluclast for 0.5 to 2 hours. It provides a method for producing a composition comprising enzyme-treated fruit.
본 발명의 일 측면인 효소 처리된 과일을 포함하는 조성물은 클로로겐산과 같은 페놀류 등을 과량 함유하고 있어, 항산화 효과가 우수하다. 또한, 본 발명에 따른 조성물은 환원당 함량이 높고, 투명도 및 색도가 높아 기호성이 우수하다. 또한, 본 발명에 따른 조성물은 지방세포 분화 억제능이 우수하여 항비만· 체중 감량 효과가 우수하다. 또한, 본 발명의 일 측면인 효소 처리 과일 조성물의 제조 방법은 과일로부터 생리활성성분을 다량 효과적으로 추출할 수 있어 경제적이다.The composition containing enzyme-treated fruit, which is one aspect of the present invention, contains an excessive amount of phenols such as chlorogenic acid, and has excellent antioxidant effect. In addition, the composition according to the present invention has a high reducing sugar content and excellent palatability due to high transparency and chromaticity. In addition, the composition according to the present invention has excellent anti-obesity and weight loss effects due to its excellent ability to inhibit adipocyte differentiation. In addition, the method for producing an enzyme-treated fruit composition, which is an aspect of the present invention, is economical because it can effectively extract a large amount of bioactive ingredients from fruit.
이하, 본 발명을 상세히 설명한다.Hereinafter, the present invention will be described in detail.
이하의 설명은 하나의 예시에 불과할 뿐, 본 발명의 권리범위가 다음 내용에 의해 제한되지 아니한다.The following description is only an example, and the scope of the present invention is not limited by the following.
또한, 본 발명의 명세서 및 청구범위에 사용된 용어 또는 단어는 통상적이거나 사전적인 의미로 한정 해석되지 아니하며, 발명자는 그 자신의 발명을 가장 최선의 방법으로 설명하기 위해 용어의 개념을 적절하게 정의할 수 있다는 원칙에 입각하여 본 발명의 기술적 사상에 부합하는 의미와 개념으로 해석되어야만 한다.In addition, the terms or words used in the specification and claims of the present invention are not to be construed as limited to their ordinary or dictionary meanings, and the inventor may appropriately define the concept of terms in order to explain his/her invention in the best way. It must be interpreted with meaning and concept consistent with the technical idea of the present invention based on the principle that it can be done.
본 발명의 명세서 전체에 있어서, 어떤 부분이 어떤 구성 요소를 "포함" 한다고 할 때, 이는 특별히 반대되는 기재가 없는 한 다른 구성 요소를 제외하는 것이 아니라 다른 구성 요소를 더 포함할 수 있는 것을 의미한다.Throughout the specification of the present invention, when a part is said to "include" a certain component, this means that it does not exclude other components but may further include other components, unless specifically stated to the contrary. .
일 측면에서, 본 발명은 효소 처리된 과일을 포함하는 조성물로서, 상기 효소는 셀루클라스트(Celluclast) 및 비스코자임(Viscozyme)을 포함하며, 상기 비스코자임은, 상기 셀루클라스트를 100 중량부로 할 때, 0.1~10 중량부로 포함되는, 효소 처리된 과일을 포함하는 조성물이다.In one aspect, the present invention is a composition comprising enzyme-treated fruit, wherein the enzyme includes Celluclast and Viscozyme, and the Viscozyme is 100 parts by weight of the Celluclast. It is a composition containing enzyme-treated fruit in an amount of 0.1 to 10 parts by weight.
본 발명의 셀루클라스트(CELLUCLASTTM, NOVOZYME社)는 셀룰라아제의 일종이며, 상기 비스코자임(ViscozymeTM, NOVOZYME社)은, 아라바나아제(arabanase), 셀룰라아제(cellulase), 베타-글루카나아제(β-glucanase), 헤미셀룰라아제(hemicellulase), 및 크실라나아제(xylanase)의 복합물을 의미할 수 있다.Celluclast (CELLUCLAST ™ , NOVOZYME) of the present invention is a type of cellulase, and Viscozyme (Viscozyme ™ (NOVOZYME)) includes arabanase, cellulase, and beta-glucanase (β). -glucanase), hemicellulase, and xylanase.
일 측면에서, 상기 비스코자임은, 상기 셀루클라스트를 100 중량부로 할 때, 0.1~10 중량부, 또는 0.1~5 중량부, 또는 0.1~3 중량부, 또는 0.5~3 중량부, 또는 0.5~2 중량부, 또는 0.8~1.2 중량부로 포함될 수 있다.In one aspect, the viscozyme is 0.1 to 10 parts by weight, or 0.1 to 5 parts by weight, or 0.1 to 3 parts by weight, or 0.5 to 3 parts by weight, or 0.5 to 100 parts by weight of the celluclast. It may be included in 2 parts by weight, or 0.8 to 1.2 parts by weight.
일 측면에서, 상기 효소 처리된 과일을 포함하는 조성물은 과일 100 중량부를 기준으로, 효소를 0.1~10 중량부, 또는 0.1~5 중량부, 또는 0.1~3 중량부로 처리한 것을 포함할 수 있다.In one aspect, the composition containing the enzyme-treated fruit may include treatment of the enzyme at 0.1 to 10 parts by weight, or 0.1 to 5 parts by weight, or 0.1 to 3 parts by weight, based on 100 parts by weight of fruit.
또한, 일 측면에서, 상기 효소 처리된 과일을 포함하는 조성물은, 비스코자임을 처리한 후 셀루클라스트를 처리한 과일을 포함할 수 있다. Additionally, in one aspect, the composition containing the enzyme-treated fruit may include fruit treated with viscozyme and then with celluclast.
일 측면에서, 상기 비스코자임과 셀루클라스트는 동시에 처리할 수도 있으나, 셀루클라스트를 처리한 후 비스코자임을 처리하는 것이 더 바람직할 수 있고, 비스코자임을 처리한 후 셀루클라스트를 처리하는 것이 가장 바람직할 수 있다.In one aspect, the Viscozyme and Celluclast may be treated at the same time, but it may be more preferable to treat Viscozyme after treating Celluclast, and it is best to treat Celluclast after treating Viscozyme. It may be desirable.
일 측면에서, 상기 과일은 사과·배·비파 등을 포함하는 인과류, 감·감귤류를 포함하는 준인과류, 복숭아·매실·살구 등을 포함하는 핵과류, 포도·바난·파인애플 등을 포함하는 장과류, 밤·호두·잣 등을 포함하는 견과류 등을 포함할 수 있으며, 바람직하게는 사과를 포함할 수 있다.In one aspect, the fruits include human fruits including apples, pears, loquats, etc., quasi-phosphorus fruits including tangerines and citrus fruits, stone fruits including peaches, plums, apricots, etc., and berries including grapes, bananas, pineapples, etc. , may include nuts including chestnuts, walnuts, pine nuts, etc., and may preferably include apples.
일 측면에서, 상기 효소 처리된 과일을 포함하는 조성물은, 항산화용 조성물을 포함하는, 효소 처리된 과일을 포함하는 조성물이다.In one aspect, the composition containing the enzyme-treated fruit is a composition containing the enzyme-treated fruit, which includes an antioxidant composition.
상기 조성물은 클로로겐산, 프로시아니딘, 갈릭산, 카페익산, 에피카테친, p-쿠마린산, 페룰산, 프로토카테츄산 등의 다양한 페놀류 등을 고함량으로 포함할 수 있다. 예컨대, 본 발명에 따른 조성물 내에 함유된 클로로겐산은 약 3.06mg/100g 이상일 수 있으며, 효소처리하지 않은 대조군 대비 최소 19배 이상 과량의 클로로겐산이 함유될 수 있다.The composition may contain a high content of various phenols such as chlorogenic acid, procyanidin, gallic acid, caffeic acid, epicatechin, p-coumaric acid, ferulic acid, and protocatechuic acid. For example, the chlorogenic acid contained in the composition according to the present invention may be about 3.06 mg/100g or more, and may contain at least 19 times an excess amount of chlorogenic acid compared to the control group that was not treated with enzymes.
일 측면에서, 본 발명의 조성물은 활성라디컬 소거능이 우수하며, 이에 우수한 항산화 효과를 나타낼 수 있다.In one aspect, the composition of the present invention has excellent active radical scavenging ability and thus can exhibit excellent antioxidant effect.
또한, 일 측면에서, 상기 효소 처리된 과일을 포함하는 조성물은, 비만 예방 또는비만 개선용 조성물을 포함할 수 있다.Additionally, in one aspect, the composition containing the enzyme-treated fruit may include a composition for preventing or improving obesity.
예컨대, 본 발명의 조성물은 지방세포의 분화를 유도하는 것으로 알려진 인자인 PAPRγ, C/EBPα 및 Prx2의 발현 억제능이 우수하여, 지방세포 분화를 효과적으로 억제할 수 있다.For example, the composition of the present invention has an excellent ability to suppress the expression of PAPRγ, C/EBPα, and Prx2, which are factors known to induce adipocyte differentiation, and can effectively inhibit adipocyte differentiation.
일 측면에서, 본 발명의 상기 효소 처리된 과일을 포함하는 조성물은 식품 조성물, 약학적 조성물 또는 화장료 조성물을 포함할 수 있다.In one aspect, the composition containing the enzyme-treated fruit of the present invention may include a food composition, pharmaceutical composition, or cosmetic composition.
상기 식품 조성물은 건강기능식품 조성물일 수 있으며, 차(茶)류, 음료류 등을 포함할 수 있다.The food composition may be a health functional food composition and may include tea, beverages, etc.
본 발명의 일 측면에 따른 식품 조성물의 제형은 특별히 한정되지 않으나, 예를 들어, 정제, 과립제, 분말제, 드링크제와 같은 액제, 캐러멜, 겔, 바 등으로 제형화될 수 있다. 각 제형의 식품 조성물은 유효 성분 이외에 해당 분야에서 통상적으로 사용되는 성분들을 제형 또는 사용 목적에 따라 당업자가 어려움 없이 적의 선정하여 배합할 수 있으며, 다른 원료와 동시에 적용할 경우 상승 효과가 일어날 수 있다.The formulation of the food composition according to one aspect of the present invention is not particularly limited, but may be formulated into, for example, tablets, granules, powders, liquids such as drinks, caramels, gels, bars, etc. In addition to the active ingredients, the food composition of each formulation can be appropriately selected and mixed by a person skilled in the art according to the formulation or purpose of use with ingredients commonly used in the field, and when applied simultaneously with other raw materials, a synergistic effect can occur.
본 발명의 일 관점에 따른 식품 조성물에 있어서, 상기 유효 성분의 투여량 결정은 당업자의 수준 내에 있으며, 투여하고자 하는 대상의 연령, 건강 상태, 합병증 등의 다양한 요인에 따라 달라질 수 있다.In the food composition according to one aspect of the present invention, the determination of the dosage of the active ingredient is within the level of those skilled in the art, and may vary depending on various factors such as the age, health condition, and complications of the subject to be administered.
상기 외에 본 발명의 일 관점에 있어서, 식품 조성물은 여러 가지 영양제, 비타민, 광물(전해질), 합성 풍미제 및 천연 풍미제 등의 풍미제, 착색제 및 증진제(치즈, 초콜릿 등), 펙트산 및 그의 염, 알긴산 및 그의 염, 유기산, 보호성 콜로이드 증점제, pH 조절제, 안정화제, 방부제, 글리세린, 알콜, 탄산 음료에 사용되는 탄산화제 등을 포함할 수 있다. 그 밖에 본 발명의 일 관점에 있어서, 기능성 식품 조성물들은 천연 과일 쥬스 및 과일 쥬스 음료 및 야채 음료의 제조를 위한 과육을 포함할 수 있다. 이러한 첨가제의 비율은 그렇게 중요하진 않지만 본 발명의 일 관점에 있어서, 조성물 100 중량부 당 0 내지 약 20 중량부의 범위에서 포함되는 것이 일반적이다.In addition to the above, in one aspect of the present invention, the food composition contains various nutrients, vitamins, minerals (electrolytes), flavoring agents such as synthetic and natural flavors, colorants and enhancers (cheese, chocolate, etc.), pectic acid, and the like. It may include salts, alginic acid and its salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohol, carbonating agents used in carbonated beverages, etc. In addition, in one aspect of the present invention, functional food compositions may include natural fruit juice and pulp for the production of fruit juice beverages and vegetable beverages. The proportion of these additives is not that critical, but in one aspect of the present invention, they are generally included in the range of 0 to about 20 parts by weight per 100 parts by weight of the composition.
본 발명의 일 측면에 따른 화장료 조성물은 화장품학적으로 허용가능한 매질 또는 기제를 함유할 수 있다. 이는 국소적용에 적합한 모든 제형으로, 예를 들면 용액, 겔, 고체 또는 반죽 무수 생성물, 수상에 유상을 분산시켜 얻은 에멀젼, 현탁액, 마이크로에멀젼, 마이크로캡슐, 미세과립구 또는 이온형(리포좀) 및/또는 비이온형의 소낭 분산제의 형태로, 또는 크림, 스킨, 로션, 파우더, 연고, 스프레이 또는 콘실 스틱의 형태로 제공될 수 있다. 이들 조성물은 당해 분야의 통상적 방법에 따라 제조될 수 있다.The cosmetic composition according to one aspect of the present invention may contain a cosmetically acceptable medium or base. These are all formulations suitable for topical application, such as solutions, gels, solid or pasty anhydrous products, emulsions obtained by dispersing the oil phase in the water phase, suspensions, microemulsions, microcapsules, microgranules or ionic forms (liposomes) and/or It may be provided in the form of a non-ionic vesicular dispersion, or in the form of a cream, skin, lotion, powder, ointment, spray or concealer stick. These compositions can be prepared according to conventional methods in the art.
본 발명의 제형이 용액 또는 유탁액인 경우에는 담체 성분으로서 용매, 용해화제 또는 유탁화제가 이용되고, 예컨대 물, 에탄올, 이소프로판올, 에틸 카보네이트, 에틸 아세테이트, 벤질 알코올, 벤질 벤조에이트, 프로필렌 글리콜, 1,3-부틸글리콜 오일, 글리세롤 지방족 에스테르, 폴리에틸렌 글리콜 또는 소르비탄의 지방산 에스테르가 있다.When the formulation of the present invention is a solution or emulsion, a solvent, solubilizing agent or emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 , 3-butyl glycol oil, glycerol aliphatic esters, polyethylene glycol or fatty acid esters of sorbitan.
본 발명의 제형이 현탁액인 경우에는 담체 성분으로서 물, 에탄올 또는 프로필렌 글리콜과 같은 액상의 희석제, 에톡실화 이소스테아릴 알코올, 폴리옥시에틸렌 소르비톨 에스테르 및 폴리옥시에틸렌 소르비탄 에스테르와 같은 현탁제, 미소결정성 셀룰로오스, 알루미늄 메타히드록시드, 벤토나이트, 아가 또는 트라칸트 등이 이용될 수 있다.When the formulation of the present invention is a suspension, the carrier ingredients include water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester, and polyoxyethylene sorbitan ester, and microcrystals. Cellulose, aluminum metahydroxide, bentonite, agar, or tracant may be used.
본 발명의 제형이 페이스트, 크림 또는 겔인 경우에는 담체 성분으로서 동물성유, 식물성유, 왁스, 파라핀, 전분, 트라칸트, 셀룰로오스 유도체, 폴리에틸렌 글리콜, 실리콘, 벤토나이트, 실리카, 탈크 또는 산화아연 등이 이용될 수 있다.When the formulation of the present invention is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier ingredient. You can.
본 발명의 제형이 파우더 또는 스프레이인 경우에는 담체 성분으로서 락토스, 탈크, 실리카, 알루미늄 히드록시드, 칼슘 실리케이트 또는 폴리아미드 파우더가 이용될 수 있고, 특히 스프레이인 경우에는 추가적으로 클로로플루오로히드로카본, 프로판/부탄 또는 디메틸 에테르와 같은 추진체를 포함할 수 있다. When the formulation of the present invention is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, or polyamide powder can be used as the carrier ingredient. In particular, when the formulation is a spray, chlorofluorohydrocarbon and propane may be used as carrier ingredients. /May contain propellants such as butane or dimethyl ether.
상기 조성물은 추가적으로 점증제를 함유할 수 있다. 본 발명의 조성물에 포함되는 점증제는 예컨대, 메틸 셀룰로스, 카르복시 메틸 셀룰로스, 카르복시 메틸 하이드록시 구아닌, 하이드록시 메틸 셀룰로스, 하이드록시에틸셀룰로스, 카르복시 비닐 폴리머, 폴리쿼터늄, 세테아릴 알콜, 스테아릭산, 카라기난 등을 사용할 수 있다. The composition may additionally contain a thickening agent. Thickening agents included in the composition of the present invention include, for example, methyl cellulose, carboxy methyl cellulose, carboxy methyl hydroxy guanine, hydroxy methyl cellulose, hydroxyethyl cellulose, carboxy vinyl polymer, polyquaternium, cetearyl alcohol, stearic acid. , carrageenan, etc. can be used.
또한, 상기 조성물은 필요에 따라 적절한 각종의 기제와 첨가제를 함유할 수 있으며, 이들 성분의 종류와 양은 발명자에 의해 용이하게 선정될 수 있다. 필요에 따라 허용 가능한 첨가제를 함유할 수 있으며, 예를 들면, 당업계에 통상적인 방부제, 색소 등의 성분을 추가로 포함할 수 있다.In addition, the composition may contain various appropriate bases and additives as needed, and the types and amounts of these components can be easily selected by the inventor. If necessary, it may contain acceptable additives, for example, it may additionally contain ingredients such as preservatives and colorants that are common in the art.
또한, 이외에 첨가해도 되는 배합 성분은 이에 한정되는 것은 아니며, 또, 상기 어느 성분도 본 발명의 목적 및 효과를 손상시키지 않는 범위 내에서 배합 가능하다.Additionally, the ingredients that may be added are not limited to this, and any of the above ingredients can be blended within the range that does not impair the purpose and effect of the present invention.
일 측면에서, 상기 조성물은 앰플, 스킨소프너, 토너, 아스트린젠트, 로션, 마사지크림, 영양크림, 모이스처크림, 핸드크림, 파운데이션, 에센스, 영양화장수, 팩, 비누, 클렌징폼, 클렌징로션, 클렌징크림, 바디로션 및 바디클린저로 이루어진 군으로부터 선택된 어느 하나 이상일 수 있다.In one aspect, the composition includes an ampoule, skin softener, toner, astringent, lotion, massage cream, nutritional cream, moisture cream, hand cream, foundation, essence, nutritional lotion, pack, soap, cleansing foam, cleansing lotion, cleansing cream, It may be any one or more selected from the group consisting of body lotion and body cleanser.
일 측면에서, 상기 조성물의 제형은 W/S, W/O, P/W, W/O/W, 또는 O/W/O일 수 있으나, 이에 제한되는 것은 아니다.In one aspect, the formulation of the composition may be W/S, W/O, P/W, W/O/W, or O/W/O, but is not limited thereto.
본 발명의 일 관점에 따른 약학 조성물은 경구 또는 비경구의 여러 가지 제형일 수 있다. 제제화할 경우에는 보통 사용하는 충진제, 증량제, 결합제, 습윤제, 붕해제, 계면활성제 등의 희석제 또는 부형제를 사용하여 조제된다. 경구투여를 위한 고형제제에는 정제, 환제, 산제, 과립제, 연질 또는 경질 캡슐제 등이 포함되며, 이러한 고형제제는 하나 이상의 화합물에 적어도 하나 이상의 부형제 예를 들면, 전분, 탄산칼슘, 수크로오스(sucrose) 또는 락토오스(lactose), 젤라틴 등을 섞어 조제된다. 또한 단순한 부형제 이외에 스테아린산 마그네슘, 탈크 등과 같은 윤활제들도 사용된다. 경구투여를 위한 액상제제로는 현탁제, 내용액제, 유제, 시럽제 등이 해당되는데 흔히 사용되는 단순 희석제인 물, 리퀴드 파라핀 이외에 여러 가지 부형제, 예를 들면 습윤제, 감미제, 방향제, 보존제 등이 포함될 수 있다. 비경구투여를 위한 제제에는 멸균된 수용액, 비수성용제, 현탁제, 유제, 동결건조제제, 좌제가 포함된다. 비수성용제, 현탁용제로는 프로필렌글리콜(propylene glycol), 폴리에틸렌 글리콜, 올리브 오일과 같은 식물성 기름, 에틸올레이트와 같은 주사 가능한 에스테르 등이 사용될 수 있다. 좌제의 기제로는 위텝솔(witepsol), 마크로골, 트윈(tween) 61, 카카오지, 라우린지, 글리세로젤라틴 등이 사용될 수 있다.The pharmaceutical composition according to one aspect of the present invention may be in various oral or parenteral dosage forms. When formulated, it is prepared using diluents or excipients such as commonly used fillers, extenders, binders, wetting agents, disintegrants, and surfactants. Solid preparations for oral administration include tablets, pills, powders, granules, soft or hard capsules, etc. These solid preparations contain one or more compounds and at least one excipient, such as starch, calcium carbonate, or sucrose. Alternatively, it is prepared by mixing lactose, gelatin, etc. In addition to simple excipients, lubricants such as magnesium stearate and talc are also used. Liquid preparations for oral administration include suspensions, oral solutions, emulsions, and syrups. In addition to the commonly used simple diluents such as water and liquid paraffin, various excipients such as wetting agents, sweeteners, fragrances, and preservatives may be included. there is. Preparations for parenteral administration include sterilized aqueous solutions, non-aqueous solutions, suspensions, emulsions, freeze-dried preparations, and suppositories. Non-aqueous solvents and suspensions may include propylene glycol, polyethylene glycol, vegetable oil such as olive oil, and injectable ester such as ethyl oleate. As a base for suppositories, witepsol, macrogol, tween 61, cacao, laurel, glycerogelatin, etc. can be used.
본 발명의 일 관점에 있어서, 조성물의 약학적 투여 형태는 이들의 약학적으로 허용 가능한 염의 형태로도 사용될 수 있고, 또한 단독으로 또는 타 약학적 활성 화합물과 결합뿐만 아니라 적당한 집합으로 사용될 수 있다. 상기 염으로는 약학적으로 허용되는 것이면 특별히 한정되지 않으며, 예를 들어 염산, 황산, 질산, 인산, 불화수소산, 브롬화수소산, 포름산 아세트산, 타르타르산, 젖산, 시트르산, 푸마르산, 말레산, 숙신산, 메탄술폰산, 벤젠술폰산, 톨루엔술폰산, 나프탈렌술폰산 등을 사용할 수 있다. In one aspect of the present invention, the pharmaceutical dosage form of the composition may be used in the form of a pharmaceutically acceptable salt thereof, and may be used alone or in combination with other pharmaceutically active compounds, as well as in an appropriate collection. The salt is not particularly limited as long as it is pharmaceutically acceptable, and examples include hydrochloric acid, sulfuric acid, nitric acid, phosphoric acid, hydrofluoric acid, hydrobromic acid, formic acid, acetic acid, tartaric acid, lactic acid, citric acid, fumaric acid, maleic acid, succinic acid, and methanesulfonic acid. , benzenesulfonic acid, toluenesulfonic acid, naphthalenesulfonic acid, etc. can be used.
본 발명의 일 관점에 따른 조성물은, 쥐, 생쥐, 가축, 인간 등의 포유동물에 비경구, 경구 등의 다양한 경로로 투여될 수 있다.The composition according to one aspect of the present invention can be administered to mammals such as rats, mice, livestock, and humans through various routes such as parenteral and oral routes.
일 측면에서, 본 발명은 과일에 셀루클라스트(Celluclast) 및 비스코자임(Viscozyme)을 처리하는 단계를 포함하는, 효소 처리된 과일을 포함하는 조성물의 제조 방법이다.In one aspect, the present invention is a method for producing a composition containing enzyme-treated fruit, comprising the step of treating the fruit with Celluclast and Viscozyme.
또한, 본 발명은 일 측면에서 과일에 셀루클라스트(Celluclast) 및 비스코자임(Viscozyme)을 처리하는 단계를 포함하는, 과일 추출물의 제조 방법이다.In addition, in one aspect, the present invention is a method for producing fruit extract, comprising the step of treating fruit with Celluclast and Viscozyme.
일 측면에서, 상기 방법은 과일에 비스코자임을 1~10시간 동안 처리한 후, 비스코자임을 처리한 과일에 셀루클라스트를 1~10시간 동안 처리하는 것을 포함할 수 있다.In one aspect, the method may include treating the fruit with Viscozyme for 1 to 10 hours and then treating the Viscozyme-treated fruit with Celluclast for 1 to 10 hours.
일 측면에서, 상기 비스코자임의 처리 시간은 2~8시간, 또는 3~7시간, 또는 4~6시간일 수 있으나, 이에 제한되는 것은 아니다.In one aspect, the processing time of the viscozyme may be 2 to 8 hours, or 3 to 7 hours, or 4 to 6 hours, but is not limited thereto.
또한, 상기 셀루클라스트의 처리시간은 2시간 이상, 3시간 이상, 4시간 이상, 5시간 이상, 또는 6시간 이상일 수 있으며, 2~10시간, 또는 3~10시간, 또는 4~10시간,또는 5~10 시간, 또는 6~10 시간일 수 있으나, 이에 제한되는 것은 아니다.In addition, the processing time of the celluclast may be 2 hours or more, 3 hours or more, 4 hours or more, 5 hours or more, or 6 hours or more, 2 to 10 hours, or 3 to 10 hours, or 4 to 10 hours, Alternatively, it may be 5 to 10 hours, or 6 to 10 hours, but is not limited thereto.
이하 제조예 및 실시예를 통하여 본 발명에 대해 구체적으로 설명하기로 한다. 이들 예는 단지 본 발명을 예시하기 위한 것이므로, 본 발명의 범위가 이들 예에 의해 제한되는 것은 아니다.Hereinafter, the present invention will be described in detail through preparation examples and examples. These examples are merely for illustrating the present invention, and the scope of the present invention is not limited by these examples.
[제조예] 효소 처리 과일 추출물의 제조[Preparation example] Preparation of enzyme-treated fruit extract
아래 공정에 의해 사과의 효소 처리 과일 추출물을 제조하였다. 구체적으로 사과를 수세하고, 슬라이스로 제조하였다. 그 후, 약 18~22도씨(℃)에서 효소를 처리했다. 효소 처리는 과일 100 중량부에 대하여 비스코자임 0.1 중량부와 셀루클라스트 1중량부를 처리하였다. 이 때 비스코자임을 먼저 약 5시간 처리한 후, 셀루클라스트를 약 1시간 동안 처리하여 효소 처리 과일 추출물을 제조하였다.Enzyme-treated apple fruit extract was prepared by the process below. Specifically, apples were washed and sliced. Afterwards, the enzyme was treated at about 18 to 22 degrees Celsius. Enzyme treatment was performed with 0.1 part by weight of Viscozyme and 1 part by weight of Celluclast per 100 parts by weight of fruit. At this time, viscozyme was first treated for about 5 hours, and then celluclast was treated for about 1 hour to prepare an enzyme-treated fruit extract.
[비교 제조예] [Comparative manufacturing example]
아래 표에 표시한 바와 같이, 조건을 변경하여 비교 제조예들을 제조하였다. 하기 표에 표시된 내용 외에는 제조예와 동일한 조건하에서 추출물을 제조하였다.As shown in the table below, comparative preparation examples were prepared by changing the conditions. The extract was prepared under the same conditions as in the preparation example except for the information indicated in the table below.
[비교 제조예 A] 효소 종류를 변경한 제조예[Comparative Preparation Example A] Preparation example in which the type of enzyme was changed
비스코자임을 처리하지 않은 군(비교예 1), 셀루클라스트를 처리하지 않은 군(비교예 2), 비스코자임 대신 쉐어자임을 처리한 군(비교예 3), 셀루클라스트 대신 쉐어자임(ShearzymeTM, NOVOZYME社)을 처리한 군(비교예 4)을 제조하였다.Group not treated with Viscozyme (Comparative Example 1), group not treated with Celluclast (Comparative Example 2), group treated with Shearzyme instead of Viscozyme (Comparative Example 3), Shearzyme instead of Celluclast A group treated with TM (NOVOZYME) (Comparative Example 4) was prepared.
[비교 제조예 B] 효소 처리 순서를 변경한 제조예[Comparative Preparation Example B] Preparation example in which the enzyme treatment sequence was changed
셀루클라스트와 비스코자임을 동시에 처리하고, 약 6시간 동안 처리한 군(비교군 5), 셀루클라스트를 처리한 후 비스코자임을 처리한 군(비교군 6)을 제조하였다.A group treated with Celluclast and Viscozyme simultaneously and treated for about 6 hours (comparative group 5), and a group treated with Celluclast and then treated with Viscozyme (comparative group 6) were prepared.
[비교 제조예 C] 효소 처리량을 변경한 제조예[Comparative Preparation Example C] Preparation example in which the enzyme treatment amount was changed
아래 표와 같이 효소 처리량 조건을 달리하여 추출물을 제조하였다. Extracts were prepared under different enzyme treatment conditions as shown in the table below.
※ 과일 총 중량 기준 함량※ Content based on total weight of fruit
[비교 제조예 D] 효소 처리 시간을 변경한 제조예[Comparative Preparation Example D] Preparation example in which the enzyme treatment time was changed
아래 표와 같이 효소 시간 조건을 달리하여 추출물을 제조하였다. Extracts were prepared under different enzyme time conditions as shown in the table below.
[실험예] [Experimental example]
[실험예 1] 사과 추출물 수율[Experimental Example 1] Apple extract yield
제조예와 비교예들에 대하여 사과 추출물 수율을 측정하였다. 그 결과는 아래 표 3에 표시된 바와 같다.Apple extract yields were measured for preparation examples and comparative examples. The results are as shown in Table 3 below.
[실험예 2] 환원당 함량 및 색도 [Experimental Example 2] Reducing sugar content and chromaticity
제조예와 비교예의 사과 추출물에 대하여 환원당의 함량을 측정하였다. 그 결과는 아래 표 4에 표시된 바와 같다. 즉 본 발명에 따른 추출물의 경우 단맛을 부여하는 환원당 함량이 높아 기호도가 우수함을 알 수 있다. The content of reducing sugar was measured for the apple extracts of Preparation Examples and Comparative Examples. The results are as shown in Table 4 below. In other words, it can be seen that the extract according to the present invention has a high content of reducing sugar, which imparts sweetness, and has excellent preference.
[실험예 3] 추출물의 안정성 측정 [Experimental Example 3] Measurement of stability of extract
제조예와 비교예의 추출물에 대하여 상온에서 24시간 동안 방치한 후, 갈변 여부를 육안으로 확인하였다.The extracts of Preparation Examples and Comparative Examples were left at room temperature for 24 hours and then visually checked for browning.
그 결과, 제조예, 비교예 10, 비교예 11, 비교예 15, 비교예 16, 비교예 19, 비교예 20, 비교예 23 및 비교예 24의 추출물은 추출 직후과 비교하여 갈변현상이 거의 일어나지 않았으나, 나머지는 갈변현상이 심하게 나타남을 확인하였다.As a result, the extracts of Preparation Example, Comparative Example 10, Comparative Example 11, Comparative Example 15, Comparative Example 16, Comparative Example 19, Comparative Example 20, Comparative Example 23, and Comparative Example 24 showed little browning compared to immediately after extraction. , it was confirmed that the rest showed severe browning.
[실험예 4] 총 폴리페놀 함량 및 클로로젠산의 함량 측정[Experimental Example 4] Measurement of total polyphenol content and chlorogenic acid content
제조예와 비교예의 추출물에 대하여 총 폴리페놀 함량과 클로로젠산의 함량을 측정하였다. 그 결과는 아래 표 5에 표시된 바와 같다. 즉, 본 발명에 따른 추출물은 폴리페놀 및 클로로젠산 등의 페놀 화합물이 다량 포함되어 항산화 효과가 우수함을 알 수 있다.The total polyphenol content and chlorogenic acid content of the extracts of Preparation Examples and Comparative Examples were measured. The results are as shown in Table 5 below. That is, it can be seen that the extract according to the present invention contains a large amount of phenolic compounds such as polyphenol and chlorogenic acid, and thus has excellent antioxidant effect.
[실험예 5] 활성 라디칼 소거 활성[Experimental Example 5] Active radical scavenging activity
제조예와 비교예의 추출물에 대하여 DPPH 라디칼 소거 활성을 측정하였다. 추출물은 8mg/mL 처리하여 실험을 수행하였다. 그 결과는 아래 표 6에 표시된 바와 같다. 즉, 본 발명에 따른 추출물은 활성 라디칼 소거능이 우수하여 항산화 효과가 우수함을 알 수 있다.DPPH radical scavenging activity was measured for the extracts of Preparation Examples and Comparative Examples. The extract was treated with 8 mg/mL and the experiment was performed. The results are as shown in Table 6 below. In other words, it can be seen that the extract according to the present invention has excellent active radical scavenging ability and thus has excellent antioxidant effect.
(mg AAE/100g FW)dpph radical scavenging activity
(mg AAE/100g FW)
※AAE: ascorbic acid equivalent, FW: fresh weight※AAE: ascorbic acid equivalent, FW: fresh weight
[실험예 6] 항비만 효능 테스트[Experimental Example 6] Anti-obesity efficacy test
제조예와 비교예의 추출물에 대하여 비만세포 분화와 연관된 인자들(PPARγ, C/EBPα,PRX2)의 발현 여부를 확인하였다.The expression of factors associated with mast cell differentiation (PPARγ, C/EBPα, and PRX2) was confirmed for the extracts of the preparation examples and comparative examples.
지방세포에 제조예와 비교예 각각을 50 ng/ml 농도로 처리하여 4일 동안 배양하였다. Fat cells were treated with each of Preparation Examples and Comparative Examples at a concentration of 50 ng/ml and cultured for 4 days.
마우스 유래 3T3-L1 전 지방세포는 100mm, 35mm culture dish 와 96 well multi plast 에 각각 seeding 한 후 growth medium (high glucose DMEM+ 1% P/S + 10% FBS)에서 100% confluence 될 때 까지 배양하였다. 이후, 2일 후 지방세포 분화 유도 물질 (1 ug/mL insulin, 1uM Dex, 0.5 mM IBMX)를 함유한 growth medium을 사용하여 전지방세포를 지방세포로 분화 유도하였다.Mouse-derived 3T3-L1 pre-adipocytes were seeded in 100mm and 35mm culture dishes and 96 well multi plast, respectively, and then cultured in growth medium (high glucose DMEM + 1% P/S + 10% FBS) until 100% confluence. Then, after 2 days, differentiation of preadipocytes into adipocytes was induced using growth medium containing adipocyte differentiation-inducing substances (1 ug/mL insulin, 1uM Dex, 0.5mM IBMX).
배양한 세포는 dPBS로 세척하여 tri-solution을 이용하여 3T3-L1 세포에서 총 RNA를 추출 및 분리하였다. 5 ug RNA를 TOP scriptTMRT DRY MIX를 이용하여 complementary DNA (cDNA)를 합성하였다. Ampi GeneTM qPCR Green Mix Hi-ROX와 primer가 포함된 혼합물 9 uL와 cDNA 1 ul를 polymerase chain reaction (PCR) 사이클을 30회 사용하였다. PCR 반응 조건은 95℃ 에서 10분, 95℃에서 30초, annealing 온도에서 30초, 72℃에서 30초 후 다시 95℃에서 30초로 돌아가는 사이클을 반복하고 72℃에서 5분을 수행하였다. 중합효소 반응에 사용된 primer 정보는 표 7에 나타내었다.Cultured cells were washed with dPBS, and total RNA was extracted and isolated from 3T3-L1 cells using tri-solution. Complementary DNA (cDNA) was synthesized from 5 ug RNA using TOP scriptTMRT DRY MIX. Ampi GeneTM qPCR Green Mix 9 μL of a mixture containing Hi-ROX and primers and 1 μL of cDNA were used for 30 polymerase chain reaction (PCR) cycles. PCR reaction conditions were 95°C for 10 minutes, 30 seconds at 95°C, 30 seconds at annealing temperature, 30 seconds at 72°C, and then repeating the cycle of 30 seconds at 95°C and 5 minutes at 72°C. Information on the primers used in the polymerase reaction is shown in Table 7.
그 결과, 제조예, 비교예 10, 비교예 11, 비교예 15, 비교예 16, 비교예 19, 비교예 20, 비교예 23 및 비교예 24의 추출물을 처리한 군에서는 PPARγ, C/EBPα, 및PRX2의 발현이 억제되었고, 나머지 처리군들에서는 상기 유전자들이 발현됨을 확인할 수 있었다.As a result, in the group treated with the extracts of Preparation Example, Comparative Example 10, Comparative Example 11, Comparative Example 15, Comparative Example 16, Comparative Example 19, Comparative Example 20, Comparative Example 23, and Comparative Example 24, PPARγ, C/EBPα, and PRX2 expression was suppressed, and it was confirmed that the above genes were expressed in the remaining treatment groups.
[실험예 7] 과일 종류에 따른 효능 테스트[Experimental Example 7] Efficacy test according to fruit type
배, 귤, 포도, 딸기 각각에 대하여 상기 제조예 및 비교예와 동일한 방법으로 추출물을 제조한 후, 실험예 1 내지 6과 동일한 실험을 수행하였다.Extracts were prepared from each of pears, tangerines, grapes, and strawberries in the same manner as in the Preparation Examples and Comparative Examples, and then the same experiments as in Experimental Examples 1 to 6 were performed.
그 결과, 각각의 과일에 함유된 성분들의 함량에 따라, 측정된 수치는 상이하였으나, 전체적인 경향성은 사과 추출물과 유사하게 나타났다. As a result, the measured values were different depending on the content of ingredients contained in each fruit, but the overall trend was similar to that of apple extract.
다만, 추출 효율에 있어서는, 제조예와 같이 효소 처리하였을 때, 사과 추출물이 가장 극적인 효과를 얻을 수 있었고, 배, 귤, 포도, 및 딸기의 경우는 효소 처리를 하더라도 효소처리를 하지 않은 대조군 대비 효율면에서 큰 개선점은 없었다.However, in terms of extraction efficiency, apple extract achieved the most dramatic effect when treated with enzyme as in the production example, and in the case of pears, tangerines, grapes, and strawberries, even with enzyme treatment, the efficiency was higher than that of the control group without enzyme treatment. There was no significant improvement in this respect.
[실험예 8] 독성 테스트[Experimental Example 8] Toxicity test
제조예와 비교예에 의한 세포 독성을 확인하기 위해 MTT 분석법을 이용하여 3T3-L1세포에서 확인하였다. 2일 동안 다양한 농도(10ng/ml, 50ng/ml, 100ng/ml)에서 세포 독성 여부를 알아본 결과 모든 추출물에 대하여 독성이 나타나지 않았다. To confirm the cytotoxicity of the preparation examples and comparative examples, it was confirmed in 3T3-L1 cells using the MTT assay. As a result of examining cytotoxicity at various concentrations (10ng/ml, 50ng/ml, 100ng/ml) for 2 days, no toxicity was found for all extracts.
Claims (8)
상기 효소는 셀루클라스트(Celluclast) 및 비스코자임(Viscozyme)을 포함하며,
상기 비스코자임은, 상기 셀루클라스트를 100 중량부로 할 때, 0.1~10 중량부로 포함되는, 효소 처리된 과일을 포함하는 조성물.A composition comprising enzyme-treated fruit,
The enzymes include Celluclast and Viscozyme,
The viscozyme is contained in an amount of 0.1 to 10 parts by weight based on 100 parts by weight of the celluclast. A composition containing enzyme-treated fruit.
상기 효소 처리된 과일을 포함하는 조성물은,
비스코자임을 처리한 후 셀루클라스트를 처리한 과일을 포함하는, 효소 처리된 과일을 포함하는 조성물.According to paragraph 1,
A composition containing the enzyme-treated fruit,
A composition comprising enzyme-treated fruit, comprising fruit treated with viscozyme followed by celluclast treatment.
상기 과일은 사과를 포함하는, 효소 처리된 과일을 포함하는 조성물.According to paragraph 1,
A composition comprising enzyme-treated fruit, wherein the fruit includes apples.
상기 효소 처리된 과일을 포함하는 조성물은,
항산화용 조성물을 포함하는, 효소 처리된 과일을 포함하는 조성물.According to paragraph 1,
A composition containing the enzyme-treated fruit,
A composition comprising enzyme-treated fruit, comprising an antioxidant composition.
상기 효소 처리된 과일을 포함하는 조성물은,
비만 예방용 또는 개선용 조성물을 포함하는, 효소 처리된 과일을 포함하는 조성물.According to paragraph 1,
A composition containing the enzyme-treated fruit,
A composition comprising enzyme-treated fruit, comprising a composition for preventing or improving obesity.
상기 효소 처리된 과일을 포함하는 조성물은,
식품 조성물, 약학적 조성물 또는 화장료 조성물을 포함하는, 효소 처리된 과일을 포함하는 조성물.According to paragraph 1,
A composition containing the enzyme-treated fruit,
Compositions comprising enzyme-treated fruit, including food compositions, pharmaceutical compositions or cosmetic compositions.
상기 방법은,
과일에 셀루클라스트(Celluclast) 및 비스코자임(Viscozyme)을 처리하는 단계를 포함하는, 효소 처리된 과일을 포함하는 조성물의 제조 방법.A method for producing a composition comprising the enzyme-treated fruit of any one of claims 1 and 6, comprising:
The above method is,
A method for producing a composition comprising enzyme-treated fruit, comprising treating the fruit with Celluclast and Viscozyme.
상기 방법은,
과일에 비스코자임을 1~10시간 동안 처리한 후,
비스코자임을 처리한 과일에 셀루클라스트를 1~10시간 동안 처리하는 것을 포함하는, 효소 처리된 과일을 포함하는 조성물의 제조 방법.In clause 7,
The above method is,
After treating the fruit with Viscozyme for 1 to 10 hours,
A method for producing a composition comprising enzyme-treated fruit, comprising treating the viscozyme-treated fruit with celluclast for 1 to 10 hours.
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| KR20150127726A (en) | 2013-03-15 | 2015-11-17 | 아크조 노벨 케미칼즈 인터내셔널 비.브이. | Bioactive compositions from fruit and methods for their production |
-
2022
- 2022-03-11 KR KR1020220030496A patent/KR20230134035A/en unknown
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20150127726A (en) | 2013-03-15 | 2015-11-17 | 아크조 노벨 케미칼즈 인터내셔널 비.브이. | Bioactive compositions from fruit and methods for their production |
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