KR20230120646A - 멀티시스트론성 rna 백신 및 이의 용도 - Google Patents

멀티시스트론성 rna 백신 및 이의 용도 Download PDF

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KR20230120646A
KR20230120646A KR1020237021041A KR20237021041A KR20230120646A KR 20230120646 A KR20230120646 A KR 20230120646A KR 1020237021041 A KR1020237021041 A KR 1020237021041A KR 20237021041 A KR20237021041 A KR 20237021041A KR 20230120646 A KR20230120646 A KR 20230120646A
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nucleotide sequence
sequence encoding
operably linked
antigen
promoter
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피라다 알렌
소자 이브나 드
잉샤 웬
수크마니 베디
쳉 창
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세퀴러스 인코포레이티드
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KR1020237021041A 2020-12-02 2021-12-02 멀티시스트론성 rna 백신 및 이의 용도 Pending KR20230120646A (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202063120362P 2020-12-02 2020-12-02
US63/120,362 2020-12-02
PCT/IB2021/061203 WO2022118226A1 (en) 2020-12-02 2021-12-02 Multicistronic rna vaccines and uses thereof

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KR20230120646A true KR20230120646A (ko) 2023-08-17

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US (1) US20240024455A1 (https=)
EP (1) EP4255446A4 (https=)
JP (1) JP2023551982A (https=)
KR (1) KR20230120646A (https=)
CN (1) CN117222415A (https=)
AU (1) AU2021392077A1 (https=)
CA (1) CA3199996A1 (https=)
IL (1) IL303315A (https=)
WO (1) WO2022118226A1 (https=)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL296752A (en) * 2020-04-03 2022-11-01 Gritstone Bio Inc Infectious disease antigens and vaccines
US20240009296A1 (en) * 2022-07-10 2024-01-11 Pfizer Inc. Self-amplifying rna encoding an influenza virus antigen
JP2025528112A (ja) * 2022-08-08 2025-08-26 セキラス インコーポレイテッド Rnaコードウイルス様粒子及びその使用
TW202438672A (zh) * 2023-01-31 2024-10-01 美商亞克圖羅斯醫療公司 四價流感疫苗之方法及組成物
KR20250140103A (ko) * 2023-02-09 2025-09-24 화이자 인코포레이티드 핵산 및 이의 용도
AU2024271830A1 (en) * 2023-05-12 2025-11-27 Seqirus Inc. Vaccine adjuvants
CN119101681A (zh) * 2023-06-09 2024-12-10 仁景(苏州)生物科技有限公司 表达可调控的工程化rna分子
EP4727580A1 (en) * 2023-06-16 2026-04-22 Seqirus Inc. Virus-like particles for the treatment of sars-cov2
WO2025003979A2 (en) * 2023-06-30 2025-01-02 Seqirus Inc. Combination rna vaccine
CN121752291A (zh) * 2023-06-30 2026-03-27 思齐乐私人有限公司 SARS-CoV-2 RNA疫苗及其用途
WO2025177220A1 (en) * 2024-02-21 2025-08-28 Seqirus Inc. Alternative self-amplifying rna
WO2025217136A2 (en) * 2024-04-08 2025-10-16 Amplitude Therapeutics, Inc. Trans-amplifying rnas encoding immunomodulators
WO2025215618A1 (en) * 2024-04-12 2025-10-16 Seqirus Inc. Methods and reagents for treating or preventing ebv
WO2025230868A1 (en) * 2024-04-29 2025-11-06 Trustees Of Boston University Self-amplifying rna and methods
WO2025257691A1 (en) * 2024-06-10 2025-12-18 Seqirus Inc. Potency assay

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030232324A1 (en) * 2001-05-31 2003-12-18 Chiron Corporation Chimeric alphavirus replicon particles
EP2848692B1 (en) * 2004-05-21 2017-08-16 Novartis Vaccines and Diagnostics, Inc. Alphavirus vectors for influenza virus vaccines
CA2766907A1 (en) * 2009-07-06 2011-01-13 Novartis Ag Self replicating rna molecules and uses thereof
MX350258B (es) * 2011-07-06 2017-08-31 Novartis Ag Emulsiones cationicas de aceite en agua.
RU2014118727A (ru) * 2011-10-11 2015-11-20 Новартис Аг Рекомбинантные самореплицирующиеся полицистронные молекулы рнк
EP3015475A1 (en) * 2014-10-31 2016-05-04 Novartis AG Mammalian cells expressing cytomegalovirus antigens
BE1024796B9 (fr) * 2016-06-02 2019-01-08 Glaxosmithkline Biologicals Sa Constructions antigeniques du virus zika
WO2020028612A1 (en) * 2018-08-01 2020-02-06 Massachusetts Institute Of Technology Rna-based regulatory technologies for mirna sensors
US12083174B2 (en) * 2018-08-17 2024-09-10 Glaxosmithkline Biologicals Sa Immunogenic compositions and uses thereof

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JP2023551982A (ja) 2023-12-13
AU2021392077A9 (en) 2024-05-23
EP4255446A4 (en) 2025-05-14
CN117222415A (zh) 2023-12-12
AU2021392077A1 (en) 2023-07-13
US20240024455A1 (en) 2024-01-25
CA3199996A1 (en) 2022-06-09
EP4255446A1 (en) 2023-10-11
WO2022118226A1 (en) 2022-06-09
IL303315A (en) 2023-07-01

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