KR20230105423A - Anti-bacterial composition comprising Artemisia argyi H. extract - Google Patents

Abstract

The present invention relates to an antibacterial composition containing an Artemisia argyi extract as an active ingredient, and as a result of the cytotoxicity test of the Artemisia argyi extract, the efficacy of each extraction solvent, and comparative experiments with competing substances showing antibacterial effects, the safe and excellent antibacterial effect of the Artemisia argyi extract was confirmed, and thus, the composition of the present invention can be usefully used as the antibacterial composition that can be safely applied to the human body without side effects.

Description

Antibacterial composition containing Artemisia argyi H. extract as an active ingredient {Anti-bacterial composition comprising Artemisia argyi H. extract}

The present invention relates to an antibacterial composition containing an extract of Artemisia Artemisia as an active ingredient.

Infection refers to the process by which a pathogen capable of causing a disease infiltrates a host and proceeds with the proliferation of the pathogen. Symptoms of infection include fever and cough, which appear as defense mechanisms of the host when toxic substances are expressed by the pathogen in the infected host.

Infectious diseases occur when the immune system is weakened, when the pathogen is highly toxic, or when exposed to a large amount of pathogens, the body's immune system fails to function properly and symptoms of infection appear.

A woman's vagina is a passageway between the outside and the uterus, so it is easily exposed to bacteria and has high humidity and temperature, making it a good environment for bacteria to proliferate. Under normal conditions, many lactic acid bacteria live inside the vagina, keeping the inside of the vagina slightly acidic and suppressing the proliferation of pathogens. When the balance of the bacterial flora in the vagina is disturbed for such reasons, vaginitis may occur due to the proliferation of opportunistic pathogens, which are normally suppressed in the vagina under normal conditions.

Bacterial vaginosis, Candida vaginitis, Trichomonas vaginitis, and atrophic vaginitis have been reported for vaginitis.

The most common disease is bacterial vaginosis, caused by Gardnerella vaginalis. This vaginitis is caused by the overgrowth of anaerobic bacteria, which normally exist in less than 1%. When the lactic acid flora is lost for various reasons, regeneration of the normal vaginal flora becomes difficult, so bacterial vaginosis often recurs. In addition, women with bacterial vaginosis have an increased frequency of serious sequelae such as pelvic inflammatory disease (PID), PID after miscarriage, postoperative cuff cellulitis, and abnormal pap smears. Risks include preterm labor, premature birth, chorioamnionitis, and endometritis after caesarean section.

In order to prevent such female diseases, cleaners and detergents are often used. Commonly used cleaners to prevent vaginitis include fatty acids or distilled lauryl alcohol, emollients, thickeners, nutrients, foaming agents, organic disinfectants, etc. Synthetic products have been released. In addition, there are surfactants with detergency and povidone-iodine as pharmaceuticals as main components.

Povidone-iodine, a disinfectant produced by the chemical synthesis described above, is known to be effective in preventing and treating vaginal infections such as Candida vaginitis, Trichomonas vaginitis, and other vaginal infections. It is known that it can cause contact dermatitis, etc., and it can cause hypothyroidism by increasing the level of inorganic iodine in the vagina or total iodine in the blood.

As a conventional technique for solving the side effects caused by the use of a disinfectant as a medicine as described above, in the case of a healthy woman, the pH of the vagina is maintained at pH 3.2 by the secretion of lactic acid by beneficial microorganisms in the vagina, and at such a low acidity, most of the Based on the fact that harmful bacteria are suppressed or killed, a pH 3.5 buffer solution formulation with lactic acid as the main component and lactoserum extracted from milk has been proposed. It is an intravaginal titratable acidity maintainer that maintains normal vaginal acidity, and it is difficult to expect fundamental treatment and prevention.

Also, as a common treatment for vaginitis, polyene drugs include nyststin and amphotericinB. Azole drugs include imidazole drugs such as ketoconazole and miconazole and triazole drugs such as itraconazole and fluconazole. (Korean Publication No. 10-2007-0009008)

Even when appropriate antibacterial agents are used, there are cases in which resistant bacteria are generated due to the use of inappropriate dosage or usage, making treatment difficult. Side effects such as odor may occur.

Artemisia argyi H. is a local specialty of Namhae-gun and is a kind of Yellow Sea mugwort, and is registered as Varieties Protection No. Island mugwort has been used for food or medicine since ancient times, and it is known that there is a significant difference from Ganghwa medicinal mugwort, which is widely used as a cosmetic material, due to its different shape, fragrance, and ingredients.

On the other hand, for the treatment of vaginitis, a sanitary napkin application composition prepared using herbal medicines (Korean Patent Publication No. 10-2001-0014909), a detergent for local washing using agar (Korean Registered Patent No. 10-1979-0001672), and female local cleansing Regarding the composition for use, a feminine cleanser composition that is easy to clean and safe for mucous membranes (Korean Patent Application No. 10-2004-0072503), a feminine cleanser composition containing bamboo salt and composite herbal extracts (Korean Patent Application No. 10-20040085458) ), vaginal cleanser composition containing pomegranate peel extract and method for manufacturing the same (Korean Patent Application No. 10-2004-0085204), herbal composition for female local cleansing containing gosam, ae-yeop, peony and red ginseng (Korean Patent Application No. 10-2004). 2009-0023172) and the like are disclosed.

As described above, various drugs have been proposed as methods for vaginal washing and treatment of abnormal symptoms, but drugs containing non-irritating natural extracts have not yet been presented, considering that the site of use is the most sensitive mucous membrane to external substances. situation is insufficient. Therefore, it is a reality that requires the development of new technologies to meet the continuous demand for compositions containing natural extracts without irritation and side effects.

<R&D research project information related to this application>

Assignment number: 2020-DD-RD-0528

Specialized research management institution: R&D Special Zone Promotion Foundation

Research Project Name: 2020 R&D Special Zone Fostering (R&D)

Task name: Development of a feminine hygiene product based on the analysis of the antibiotic effect of domestic artemisia argyi H. extract and mandarin (Citrus unshiu Marc. var. miyagawa) essential oil

Organized by: Daejeon University Industry-University Cooperation Foundation

General manager: Lim Mi-hye

Research period: 2020. 10. 15. ~ 2021. 04. 14.

Korean Patent Application No. 10-2004-0085458 Republic of Korea Patent Application No. 10-2004-0085204

An object of the present invention to solve the above problems is to provide an antibacterial composition containing an extract of Artemisia Artemisia as an active ingredient, and to verify the antibacterial effect of the extract of Artemisia Artemisia by solvent, thereby preventing the bacteria causing vaginitis. It is to provide an antimicrobial composition effective in suppression.

The technical problem to be achieved by the present invention is not limited to the above-mentioned technical problem, and other technical problems not mentioned can be clearly understood by those skilled in the art from the description below. There will be.

One embodiment of the present invention for achieving the above object provides a naturally extracted antibacterial composition containing an extract of Artemisia Artemisia as an active ingredient.

In addition, the present invention provides a feminine cleanser composition containing an extract of wormwood as an active ingredient.

In addition, the present invention provides a pharmaceutical composition for preventing or treating vaginitis containing an extract of Artemisia Artemisia as an active ingredient.

The effect of the present invention according to the configuration as described above relates to an antibacterial composition containing an extract of Artemisia japonica as an active ingredient, and has an antibacterial effect such as G. vaginalis, which is a major causative agent of vaginitis.

In addition, the antibacterial composition containing the wormwood extract of the present invention as an active ingredient can be usefully used as a feminine cleanser composition or a pharmaceutical composition for preventing or treating vaginitis.

The effects of the present invention are not limited to the above effects, and should be understood to include all effects that can be inferred from the detailed description of the present invention or the configuration of the invention described in the claims.

Figure 1 shows the result of measuring the inhibition zone by performing the disc diffusion method on 5 strains in order to confirm the antibacterial effect of the hot water extract, essential oil, hydrosol and mandarin essential oil of Artemisia Artemisia, and the antibacterial activity of essential oil of Artemisia Artemisia. is the result of the experiment.
Figure 2 is an experiment result comparing the antibacterial activity of Chinese yellow sea wormwood essential oil of the same species and β-caryophyllene and 1,8-cineole components, which are the main components of yellow sea wormwood, by disc diffusion method in order to compare the antibacterial activity of wormwood essential oil. .
Figure 3 is an experimental result comparing the beneficial bacteria preservation effect of two types of marker substances: Artemisia wormwood essential oil, Chinese yellow sea wormwood essential oil and mugwort essential oil.
Figure 4 is an experimental result of measuring the MIC for P. aeruginosa, S. aureus, E. coli, G. vaginalis and C. albicans in order to quantitatively analyze the antibacterial activity of essential oil of Artemisia.

Hereinafter, the present invention will be described with reference to the accompanying drawings. However, the present invention can be implemented in many different forms, and therefore is not limited to the embodiments described herein. And in order to clearly explain the present invention in the drawings, parts irrelevant to the description are omitted, and similar reference numerals are attached to similar parts throughout the specification.

Throughout the specification, when a part is said to be "connected (connected, contacted, combined)" with another part, this is not only "directly connected", but also "indirectly connected" with another member in between. "Including cases where In addition, when a part "includes" a certain component, it means that it may further include other components without excluding other components unless otherwise stated.

Terms used in this specification are only used to describe specific embodiments, and are not intended to limit the present invention. Singular expressions include plural expressions unless the context clearly dictates otherwise. In this specification, terms such as "include" or "have" are intended to indicate that there is a feature, number, step, operation, component, part, or combination thereof described in the specification, but one or more other features It should be understood that the presence or addition of numbers, steps, operations, components, parts, or combinations thereof is not precluded.

Hereinafter, the present invention will be described in detail with reference to the accompanying drawings.

The present invention relates to an antibacterial composition containing an extract of island wormwood as an active ingredient.

A composition containing the island wormwood extract of the present invention as an active ingredient may be provided in the form of an antibacterial cosmetic composition. When used as cosmetics, it may be provided in the form of a general emulsified formulation or solubilized formulation, lotion such as softening lotion or nourishing lotion, emulsion such as facial lotion, body lotion, etc., cream such as nourishing cream, moisture cream, eye cream, etc. It may have formulations such as essence, cosmetic ointment, spray, gel, pack, sunscreen, makeup base, foundation, powder, cleansing cream, cleansing lotion, cleansing oil, cleansing foam, soap, cleanser such as body wash,

In addition, the cosmetics include fatty substances, organic solvents, solubilizers, thickeners and gelling agents, softeners, antioxidants, suspending agents, stabilizers, foaming agents, fragrances, surfactants, water, emulsifiers, fillers, chelating agents, preservatives, vitamins, It may additionally contain adjuvants commonly used in the field of cosmetology, such as occlusive agents, humectants, oils, dyes, pigments, chemical agents, lipid vesicles or any other ingredient commonly used in cosmetics.

The composition containing the island wormwood extract as an active ingredient of the present invention relates to antibacterial health food.

In addition, the 'health food' refers to a food prepared by adding wormwood extract to food materials such as beverages, teas, spices, gum, confectionery, etc., or encapsulated, powdered, suspended, etc., and when ingested, certain health effects However, unlike general drugs, it has the advantage of not having side effects that can occur when taking drugs for a long time using food as a raw material.

Since the health food of the present invention can be consumed on a daily basis, it is very useful because a continuous antibacterial effect can be expected without side effects.

The health food of the present invention may further include ingredients commonly added during food preparation, such as proteins, carbohydrates, fats, nutrients, seasonings, and flavors, as well as the extract of Artemisia.

Examples of such carbohydrates include monosaccharides such as glucose, fructose, and the like; disaccharides such as maltose, sucrose, oligosaccharides and the like; and polysaccharides such as conventional sugars such as dextrins and cyclodextrins and sugar alcohols such as xylitol, sorbitol and erythritol. As flavoring agents, natural flavoring agents [thaumatin, stevia extract (eg, rebaudioside A, glycyrrhizin, etc.]) and synthetic flavoring agents (saccharin, aspartame, etc.) can be used.

In addition, when the food composition is prepared as a drink, citric acid, liquid fructose, sugar, glucose, acetic acid, malic acid, fruit juice, Eucommia extract, jujube extract, licorice extract, etc. may be further included in addition to the wormwood extract of the present invention.

The composition containing the island wormwood extract of the present invention as an active ingredient can be used as a pharmaceutical composition, and in this case, it can be administered orally or parenterally, preferably parenteral administration, more preferably topical application or washing. It is applied by topical application.

The pharmaceutical composition of the present invention is prepared in unit dosage form or multi-dose by formulating using pharmaceutically acceptable carriers and/or excipients according to a method that can be easily performed by those skilled in the art. It can be prepared by incorporating into a container.

At this time, the formulation may be in the form of a solution, suspension, syrup or emulsion in an oil or aqueous medium, or in the form of an extractor, powder, powder, granule, tablet or capsule, and may additionally contain a dispersing agent or stabilizer.

Preparation Example 1. Sample extraction

Hot water extraction was performed by adding 1 liter of distilled water to 100 g of Artemisia Artemisia, reflux extraction was performed with 1 liter of 70% ethanol for 3 hours, and each extracted filtrate was concentrated under reduced pressure with a rotary vacuum evaporator, and the concentrated solution was freeze dryer. was freeze-dried. The hydrosol was prepared by distilling the hot water extract. Hot water extract (Artemisia argyi distilled water extract, below, AAD) was 11.77 g (yield: 11.77%), 70% ethanol extract (Artemisia argyi ethanol extract, below, AAE, essential oil) was 9.20 g (yield: 9.20%). While stored in a cryogenic freezer (-80 ° C), it was diluted in distilled water to the concentration required for the experiment and used.

Example 1. Cell culture

RAW264.7 cells were cultured in a cell culture medium maintained at 37°C and 5% CO2 using DMEM medium composed of 10% fetal bovine serum and 1% penicillin-streptomycin. proceeded.

For CCD-986Sk cells, DMEM medium containing 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin was used. All cells were cultured in a cell incubator maintained at 37°C and 5% CO2 conditions, and the experiments were conducted by subculturing at a cycle of 2-3 days.

Example 2. Determination of Heavy Metal Content of Artemisia Artemisia Extract

In order to confirm the safety of the hot water extract of Artemisia Artemisia, essential oil of Artemisia Artemisia, and Artemisia Artemisia hydrosol, a heavy metal test was performed, and all of the raw materials were confirmed to be non-detected. (See Table 1)

<Table 1>

Example 3. Measurement of cell viability

RAW264.7 cells were dispensed at 1×105 cells/well in a 24 well plate and cultured for 24 hours.

After 24 hours, AAD was treated at concentrations of 50, 100, 200, and 400 μg/ml, followed by incubation for another 24 hours, and 10 μl of EZ-Cytox solution was added per 100 μl of the cell culture medium, followed by reaction in a cell incubator for 30 minutes.

After the reaction, the change in absorbance at 450 nm was measured, and cell viability relative to the control group was expressed as a percentage.

CCD-986Sk cells were dispensed in a 24 well plate at 5×10 4 cells/well and cultured for 24 hours.

After 24 hours, AAD and AAE were treated at concentrations of 50, 100, 200, and 400 μg/ml and cultured for 48 hours.

After all the incubation was completed, 10 μl of EZ-cytox solution was added per 100 μl of the culture medium and reacted in a cell incubator for 40 minutes.

After the reaction, the change in absorbance was measured at 450 nm, and cell viability relative to the control group was expressed as a percentage.

MC/9 cells were dispensed in a 48 well plate at 1×105 cells/well and cultured for 24 hours.

After 24 hours, AAD was treated at concentrations of 25, 50, 100, and 200 μg/ml and cultured for another 12 hours.

After culturing, 10 μl of EZ-Cytox solution was added per 100 μl of the culture medium and reacted in a cell incubator for 30 minutes.

After the reaction, the change in absorbance at 450 nm was measured, and the cell viability for the control group was shown in percentage in Table 2.

<Table 2>

Example 4. GC-MSD of essential oils

Wormwood cultivars contain α-thujone, which has been reported to have neurotoxicity. Therefore, we tested whether Island Artemisia contains α-thujone.

As a result of analyzing the main component content including α-thujone component through GC analysis of Artemisia Artemisia essential oil, it was confirmed that the essential oil of Artemisia Artemisia was composed of about 60 components, and α-thujone was not detected.

As a result of quantitative analysis of the main components of the essential oil of Artemisia Artemisia, it was confirmed that 1,8-cineole contained 326.1 ppm and β-caryophyllene 261.0 ppm. (See Table 3)

<Table 3>

Example 5. Antibacterial efficacy of wormwood extract

As a result of the paper disc test, in order to confirm the antibacterial effect of the obtained hot-water extract, essential oil, hydrosol and mandarin essential oil of Artemisia Artemisia, the disc diffusion method was performed on 5 strains and the inhibition zone was measured. As a result, the antimicrobial activity of essential oil of Artemisia Artemisia confirmed to be excellent. Through this, the antibacterial effect was further analyzed by the essential oil of Artemisia. (FIG. 1)

Example 6. Comparison of antibacterial activity of two indicators of essential oil of Artemisia Artemisia, essential oil of Chinese Yellow Sea Wormwood and essential oil of Yellow Sea Artemisia

In order to compare the antibacterial activity of essential oil of Artemisia Artemisia, the antibacterial activity of β-caryophyllene and 1,8-cineole, which are the main components of Chinese Yellow Sea Wormwood essential oil and Yellow Sea Artemisia, were compared by disc diffusion method.

Artemisia essential oil showed a certain amount of antibacterial activity against E. coli, S. aureus, and C. albicans, and in the case of G. vaginalis, a vaginitis bacterium, it was found to have relatively high antibacterial activity compared to essential oil of Yellow Sea Artemisia. It was confirmed that there is an antibacterial activity comparable to caryophyllen, one of the main components of Yellow Sea Wormwood (Fig. 2).

Example 7. Preservation effect of beneficial bacteria of two kinds of indicator substances of Artemisia Artemisia, essential oil of Chinese Yellow Sea Artemisia and essential oil of Artemisia

Unlike the yellow sea mugwort essential oil that inhibited beneficial bacteria, it was confirmed that it had a protective effect against L. vaginalis, a beneficial bacteria in the vagina. (FIG. 3)

Example 8. Confirmation of Minimum Antibacterial Concentration of Artemisia Essential Oil and Essential Oil of Chinese Yellow Sea Wormwood

In order to quantitatively analyze the antibacterial activity of essential oil of Artemisia, the MIC against P. aeruginosa, S. aureus, E. coli, G. vaginalis and C. albicans was measured. It was confirmed that the essential oil of Artemisia Artemisia exhibits an antibacterial effect at a low concentration. (FIG. 4)

Example 9. Detergent formulation

Anti-inflammatory effect can be expected with 5% of Artemisia wormwood extract, and 0.5% of essential oil blending was used to include antibacterial effect, including essential oil of Artemisia Artemisia.

In addition, thyme (Thymus vulgaris), rosewood (Aniba rosaeodora), geranium (Pelargonium graveolens), and tea tree (Melareuca alternifolia) essential oils, which were shown to have high antibacterial efficacy as a result of screening for the antibacterial efficacy of 40 essential oils against G. vaginalis, were tested. included

The above description of the present invention is for illustrative purposes, and those skilled in the art can understand that it can be easily modified into other specific forms without changing the technical spirit or essential features of the present invention. will be. Therefore, the embodiments described above should be understood as illustrative in all respects and not limiting. For example, each component described as a single type may be implemented in a distributed manner, and similarly, components described as distributed may be implemented in a combined form.

The scope of the present invention is indicated by the following claims, and all changes or modifications derived from the meaning and scope of the claims and equivalent concepts thereof should be construed as being included in the scope of the present invention.

Claims (11)

An antimicrobial composition comprising an extract of island wormwood as an active ingredient.
According to claim 1,
The island wormwood extract is an antimicrobial composition, characterized in that the reflux extract using ethanol.
According to claim 2,
The ethanol extract is an antimicrobial composition, characterized in that 0.01 to 20 v / v%.
According to claim 3,
The ethanol extract is an antimicrobial composition, characterized in that 0.03 to 4 v / v%.
An antibacterial external skin preparation comprising an ethanol extract of Artemisia wormwood as an active ingredient.
According to claim 5,
The antibacterial skin external preparation is an antibacterial external skin preparation, characterized in that the formulation of any one of ointments, patches, gels, creams, sprays and detergents.
A feminine cleanser composition comprising an ethanol extract of Artemisia Artemisia as an active ingredient.
According to claim 7,
The feminine cleanser composition is a feminine cleanser composition, characterized in that the formulation of any one of lotion, essence, lotion, cream, pack, gel, powder, foundation and detergent.
An antibacterial health functional food comprising an ethanol extract of Artemisia Artemisia as an active ingredient.
A pharmaceutical composition for preventing or treating vaginitis, characterized in that it comprises an ethanol extract of Artemisia Artemisia as an active ingredient.
According to claim 10,
The pharmaceutical composition for preventing or treating vaginitis is a pharmaceutical composition for preventing or treating vaginitis, characterized in that the formulation of any one of ointments, patches, gels, creams, sprays and detergents.

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