KR20230101298A - Antibacterial composition containing Salvia miltiorrhiza extract - Google Patents
Antibacterial composition containing Salvia miltiorrhiza extract Download PDFInfo
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- KR20230101298A KR20230101298A KR1020210191284A KR20210191284A KR20230101298A KR 20230101298 A KR20230101298 A KR 20230101298A KR 1020210191284 A KR1020210191284 A KR 1020210191284A KR 20210191284 A KR20210191284 A KR 20210191284A KR 20230101298 A KR20230101298 A KR 20230101298A
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- antibacterial
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- danshen
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- 239000000284 extract Substances 0.000 title claims abstract description 48
- 239000000203 mixture Substances 0.000 title claims abstract description 35
- 230000000844 anti-bacterial effect Effects 0.000 title claims abstract description 29
- 241000304195 Salvia miltiorrhiza Species 0.000 title description 2
- 235000011135 Salvia miltiorrhiza Nutrition 0.000 title description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 79
- 244000132619 red sage Species 0.000 claims abstract description 32
- WTPPRJKFRFIQKT-UHFFFAOYSA-N 1,6-dimethyl-8,9-dihydronaphtho[1,2-g][1]benzofuran-10,11-dione;1-methyl-6-methylidene-8,9-dihydro-7h-naphtho[1,2-g][1]benzofuran-10,11-dione Chemical compound O=C1C(=O)C2=C3CCCC(=C)C3=CC=C2C2=C1C(C)=CO2.O=C1C(=O)C2=C3CCC=C(C)C3=CC=C2C2=C1C(C)=CO2 WTPPRJKFRFIQKT-UHFFFAOYSA-N 0.000 claims abstract description 31
- 238000000034 method Methods 0.000 claims abstract description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 10
- 241000588724 Escherichia coli Species 0.000 claims abstract description 8
- 241000589517 Pseudomonas aeruginosa Species 0.000 claims abstract description 8
- 241000191967 Staphylococcus aureus Species 0.000 claims abstract description 8
- 238000001256 steam distillation Methods 0.000 claims abstract description 5
- 241000196324 Embryophyta Species 0.000 claims abstract description 3
- 239000007864 aqueous solution Substances 0.000 claims description 17
- 239000000243 solution Substances 0.000 claims description 15
- BSAIUMLZVGUGKX-UHFFFAOYSA-N non-2-enal Chemical compound CCCCCCC=CC=O BSAIUMLZVGUGKX-UHFFFAOYSA-N 0.000 claims description 10
- 239000007791 liquid phase Substances 0.000 claims description 8
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 claims description 6
- 239000012153 distilled water Substances 0.000 claims description 6
- 238000004519 manufacturing process Methods 0.000 claims description 5
- 241000894006 Bacteria Species 0.000 claims description 4
- 239000012141 concentrate Substances 0.000 claims description 4
- 150000001299 aldehydes Chemical class 0.000 claims description 3
- 230000001877 deodorizing effect Effects 0.000 claims description 3
- 230000000845 anti-microbial effect Effects 0.000 claims 1
- 239000004599 antimicrobial Substances 0.000 claims 1
- 235000019645 odor Nutrition 0.000 abstract description 6
- 239000000126 substance Substances 0.000 abstract description 5
- 239000002994 raw material Substances 0.000 abstract description 2
- 125000002485 formyl group Chemical class [H]C(*)=O 0.000 abstract 1
- 230000000052 comparative effect Effects 0.000 description 55
- BSAIUMLZVGUGKX-BQYQJAHWSA-N (E)-non-2-enal Chemical compound CCCCCC\C=C\C=O BSAIUMLZVGUGKX-BQYQJAHWSA-N 0.000 description 14
- VZSRBBMJRBPUNF-UHFFFAOYSA-N 2-(2,3-dihydro-1H-inden-2-ylamino)-N-[3-oxo-3-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)propyl]pyrimidine-5-carboxamide Chemical compound C1C(CC2=CC=CC=C12)NC1=NC=C(C=N1)C(=O)NCCC(N1CC2=C(CC1)NN=N2)=O VZSRBBMJRBPUNF-UHFFFAOYSA-N 0.000 description 11
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 8
- MKYBYDHXWVHEJW-UHFFFAOYSA-N N-[1-oxo-1-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)propan-2-yl]-2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidine-5-carboxamide Chemical compound O=C(C(C)NC(=O)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F)N1CC2=C(CC1)NN=N2 MKYBYDHXWVHEJW-UHFFFAOYSA-N 0.000 description 8
- AFCARXCZXQIEQB-UHFFFAOYSA-N N-[3-oxo-3-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)propyl]-2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidine-5-carboxamide Chemical compound O=C(CCNC(=O)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F)N1CC2=C(CC1)NN=N2 AFCARXCZXQIEQB-UHFFFAOYSA-N 0.000 description 8
- 239000006916 nutrient agar Substances 0.000 description 8
- NIPNSKYNPDTRPC-UHFFFAOYSA-N N-[2-oxo-2-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)ethyl]-2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidine-5-carboxamide Chemical compound O=C(CNC(=O)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F)N1CC2=C(CC1)NN=N2 NIPNSKYNPDTRPC-UHFFFAOYSA-N 0.000 description 7
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 6
- 238000004821 distillation Methods 0.000 description 6
- 238000002360 preparation method Methods 0.000 description 5
- JQMFQLVAJGZSQS-UHFFFAOYSA-N 2-[4-[2-(2,3-dihydro-1H-inden-2-ylamino)pyrimidin-5-yl]piperazin-1-yl]-N-(2-oxo-3H-1,3-benzoxazol-6-yl)acetamide Chemical compound C1C(CC2=CC=CC=C12)NC1=NC=C(C=N1)N1CCN(CC1)CC(=O)NC1=CC2=C(NC(O2)=O)C=C1 JQMFQLVAJGZSQS-UHFFFAOYSA-N 0.000 description 4
- CONKBQPVFMXDOV-QHCPKHFHSA-N 6-[(5S)-5-[[4-[2-(2,3-dihydro-1H-inden-2-ylamino)pyrimidin-5-yl]piperazin-1-yl]methyl]-2-oxo-1,3-oxazolidin-3-yl]-3H-1,3-benzoxazol-2-one Chemical compound C1C(CC2=CC=CC=C12)NC1=NC=C(C=N1)N1CCN(CC1)C[C@H]1CN(C(O1)=O)C1=CC2=C(NC(O2)=O)C=C1 CONKBQPVFMXDOV-QHCPKHFHSA-N 0.000 description 4
- 239000006142 Luria-Bertani Agar Substances 0.000 description 4
- REFJWTPEDVJJIY-UHFFFAOYSA-N Quercetin Chemical compound C=1C(O)=CC(O)=C(C(C=2O)=O)C=1OC=2C1=CC=C(O)C(O)=C1 REFJWTPEDVJJIY-UHFFFAOYSA-N 0.000 description 4
- JAWMENYCRQKKJY-UHFFFAOYSA-N [3-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-ylmethyl)-1-oxa-2,8-diazaspiro[4.5]dec-2-en-8-yl]-[2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidin-5-yl]methanone Chemical compound N1N=NC=2CN(CCC=21)CC1=NOC2(C1)CCN(CC2)C(=O)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F JAWMENYCRQKKJY-UHFFFAOYSA-N 0.000 description 4
- 238000006243 chemical reaction Methods 0.000 description 4
- HHEAADYXPMHMCT-UHFFFAOYSA-N dpph Chemical compound [O-][N+](=O)C1=CC([N+](=O)[O-])=CC([N+]([O-])=O)=C1[N]N(C=1C=CC=CC=1)C1=CC=CC=C1 HHEAADYXPMHMCT-UHFFFAOYSA-N 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 238000007654 immersion Methods 0.000 description 4
- 235000008331 Pinus X rigitaeda Nutrition 0.000 description 3
- 235000011613 Pinus brutia Nutrition 0.000 description 3
- 241000018646 Pinus brutia Species 0.000 description 3
- 230000002292 Radical scavenging effect Effects 0.000 description 3
- 238000012790 confirmation Methods 0.000 description 3
- GVJHHUAWPYXKBD-UHFFFAOYSA-N (±)-α-Tocopherol Chemical compound OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 description 2
- 241000208340 Araliaceae Species 0.000 description 2
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 2
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 description 2
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 description 2
- 235000003140 Panax quinquefolius Nutrition 0.000 description 2
- ZVOLCUVKHLEPEV-UHFFFAOYSA-N Quercetagetin Natural products C1=C(O)C(O)=CC=C1C1=C(O)C(=O)C2=C(O)C(O)=C(O)C=C2O1 ZVOLCUVKHLEPEV-UHFFFAOYSA-N 0.000 description 2
- HWTZYBCRDDUBJY-UHFFFAOYSA-N Rhynchosin Natural products C1=C(O)C(O)=CC=C1C1=C(O)C(=O)C2=CC(O)=C(O)C=C2O1 HWTZYBCRDDUBJY-UHFFFAOYSA-N 0.000 description 2
- 238000002835 absorbance Methods 0.000 description 2
- 239000000645 desinfectant Substances 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 238000009472 formulation Methods 0.000 description 2
- 235000008434 ginseng Nutrition 0.000 description 2
- 210000004209 hair Anatomy 0.000 description 2
- ZXEKIIBDNHEJCQ-UHFFFAOYSA-N isobutanol Chemical compound CC(C)CO ZXEKIIBDNHEJCQ-UHFFFAOYSA-N 0.000 description 2
- MWDZOUNAPSSOEL-UHFFFAOYSA-N kaempferol Natural products OC1=C(C(=O)c2cc(O)cc(O)c2O1)c3ccc(O)cc3 MWDZOUNAPSSOEL-UHFFFAOYSA-N 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 239000000419 plant extract Substances 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 229960001285 quercetin Drugs 0.000 description 2
- 235000005875 quercetin Nutrition 0.000 description 2
- 239000002904 solvent Substances 0.000 description 2
- 238000000527 sonication Methods 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 1
- 244000144927 Aloe barbadensis Species 0.000 description 1
- 235000002961 Aloe barbadensis Nutrition 0.000 description 1
- 240000001949 Taraxacum officinale Species 0.000 description 1
- 235000005187 Taraxacum officinale ssp. officinale Nutrition 0.000 description 1
- 229930003427 Vitamin E Natural products 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 235000011399 aloe vera Nutrition 0.000 description 1
- 230000000843 anti-fungal effect Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000006866 deterioration Effects 0.000 description 1
- MGJZITXUQXWAKY-UHFFFAOYSA-N diphenyl-(2,4,6-trinitrophenyl)iminoazanium Chemical compound [O-][N+](=O)C1=CC([N+](=O)[O-])=CC([N+]([O-])=O)=C1N=[N+](C=1C=CC=CC=1)C1=CC=CC=C1 MGJZITXUQXWAKY-UHFFFAOYSA-N 0.000 description 1
- 238000007598 dipping method Methods 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- WIGCFUFOHFEKBI-UHFFFAOYSA-N gamma-tocopherol Natural products CC(C)CCCC(C)CCCC(C)CCCC1CCC2C(C)C(O)C(C)C(C)C2O1 WIGCFUFOHFEKBI-UHFFFAOYSA-N 0.000 description 1
- 241000411851 herbal medicine Species 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000003020 moisturizing effect Effects 0.000 description 1
- 239000013641 positive control Substances 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- BDERNNFJNOPAEC-UHFFFAOYSA-N propan-1-ol Chemical compound CCCO BDERNNFJNOPAEC-UHFFFAOYSA-N 0.000 description 1
- 230000037380 skin damage Effects 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 239000011550 stock solution Substances 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 238000005292 vacuum distillation Methods 0.000 description 1
- 235000019165 vitamin E Nutrition 0.000 description 1
- 229940046009 vitamin E Drugs 0.000 description 1
- 239000011709 vitamin E Substances 0.000 description 1
- 239000000341 volatile oil Substances 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
- A61Q17/005—Antimicrobial preparations
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Mycology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Dermatology (AREA)
- Epidemiology (AREA)
- Botany (AREA)
- Birds (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Agronomy & Crop Science (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
본 발명은 단삼 추출물을 함유하는 항균 조성물에 관한 것이다. 상기 단삼 추출물은 알코올 수용액에 식물 원료를 첨가하여 침지한 시료를 저온 수증기 증류법을 이용하여 유용성 물질을 추출하는 방법을 통해 얻은 것이다. 상기 방법으로 얻은 단삼 추출물은 기존의 알코올 추출물이나 물 추출물, 초음파 처리 추출물 등에 비해 녹농균, 대장균, 및 황색포도상구균에 대한 항균능이 우수하고, 노네날 알데하이드로 유발되는 냄새의 제거 효능이 뛰어난 것으로 확인된다. The present invention relates to an antibacterial composition containing a Danshen extract. The Danshen extract is obtained by extracting useful substances from a sample immersed by adding a plant raw material to an aqueous alcohol solution using a low-temperature steam distillation method. Danshen extract obtained by the above method has excellent antibacterial activity against Pseudomonas aeruginosa, Escherichia coli, and Staphylococcus aureus compared to conventional alcohol extracts, water extracts, sonicated extracts, etc., and is confirmed to be excellent in removing odors caused by nonnal aldehyde. .
Description
본 발명은 단삼 추출물을 함유하는 항균 조성물에 관한 것이다. The present invention relates to an antibacterial composition containing a Danshen extract.
코로나 19가 심각해지며 소독 및 항균에 대한 관심이 늘어나고 있다. 이와 같이 손소독제가 일상 필수품이 되었지만, 손소독제의 사용이 보편화 되면서 소독제 안의 알코올 등과 같은 강한 화학성분으로 인하여 피부손상이 종종 발생한다. 현재 개인 위생용품으로 판매되고 있는 손소독제는 이와 같은 알코올을 기반으로 하여 제조되고 있으며 보습성을 증진시키기 위하여 알로에 베라를 첨가하여 제조된다. 알코올은 보통 60% 이상으로 제조되는데, 이 경우 우리나라의 소방법에 저촉되므로 알콜 농도를 60% 이하로 조정하여 손소독제를 제조할 필요성이 있고, 알콜 농도 저하에 따른 항균력을 보완 및 증진시키기 위한 방법이 필요하다. 또한 손소독제에 함유된 다량의 알코올의 자극적인 향 및 냄새는 소비자의 기호도를 저하시키는 근본적인 원인이 되므로 이를 상쇄할 소취활성의 효과를 가지는 우수한 소재를 선발할 필요성이 있다.As Corona 19 becomes serious, interest in disinfection and antibacterial is increasing. As such, hand sanitizers have become a daily necessity, but as the use of hand sanitizers has become common, skin damage often occurs due to strong chemical components such as alcohol in the disinfectant. Hand sanitizers currently sold as personal hygiene products are manufactured based on such alcohol, and are manufactured by adding aloe vera to enhance moisturizing properties. Alcohol is usually manufactured at 60% or more, but in this case, it is necessary to adjust the alcohol concentration to 60% or less to manufacture hand sanitizer because it is in violation of the Fire Protection Act in Korea, and a method to supplement and enhance the antibacterial power according to the decrease in alcohol concentration is needed. need. In addition, since the irritating scent and smell of a large amount of alcohol contained in hand sanitizer is a fundamental cause of deterioration of consumer's preference, there is a need to select an excellent material having a deodorizing effect to offset it.
단삼은 인삼의 형태를 닮고 빛깔이 붉어서 단삼이라고 하였다. 학명은 Salvia miltiorrhiza BUNGE이다. 높이는 40∼80㎝이고 전체에 털이 많다. 잎은 난형 또는 피침형(披針形)으로 마주 난다. 뒷면에는 털이 밀생하고 둔한 톱니가 있다. 꽃은 자주색으로 5∼6월에 피는데 층층으로 달린다. 뿌리는 한약재로 쓰인다. 탄신논과 비타민 E가 함유되어 있다. Dansam resembles ginseng in shape and is red in color, so it was called Dansam. The scientific name is Salvia miltiorrhiza BUNGE. It is 40 to 80 cm tall and has many hairs throughout. Leaves are ovate or lanceolate. The back side has dense hairs and dull sawtooth. The flowers are purple and bloom in May-June and run in layers. The root is used as herbal medicine. It contains tancinnon and vitamin E.
이에 본 발명자들은 다양한 식물 추출물로부터 항균 소재들을 찾아내어 이들 추출물의 최적의 추출방법을 적용해냄으로써 우수한 항균 활성을 갖는 조성물을 제조하여 본 발명을 완성하게 되었다. Accordingly, the present inventors have completed the present invention by preparing a composition having excellent antibacterial activity by finding antibacterial materials from various plant extracts and applying the optimal extraction method of these extracts.
본 발명의 목적은 단삼 추출물을 함유하는 항균 조성물을 제공하는 데에 있다. An object of the present invention is to provide an antibacterial composition containing a Danshen extract.
본 발명은 단삼 추출물을 함유하는 항균용 조성물의 제조방법에 관한 것으로서, The present invention relates to a method for preparing an antibacterial composition containing a Danshen extract,
바람직하게는 (제1단계) 단삼을 알코올 수용액에 침지하는 단계; Preferably (first step) immersing Danshen in an aqueous alcohol solution;
(제2단계) 알코올 수용액에 침지된 단삼을 저압 수증기 증류법으로 증류하는 단계;(Second step) distilling Danshen immersed in an aqueous alcohol solution by low pressure steam distillation;
(제3단계) 증류된 증기를 수집하고 응축하여 액상을 얻는 단계; 및,(Third Step) Collecting and condensing the distilled vapor to obtain a liquid phase; and,
(제4단계) 상기 액상을 감압증류하여 농축물을 얻는 단계;(Fourth step) distilling the liquid phase under reduced pressure to obtain a concentrate;
를 포함할 수 있다.can include
상기 단삼은 단삼의 뿌리, 잎, 줄기 등의 어떤 것이라도 사용가능하며, 생것이나 건조된 것 어떤 것이든 사용가능하다. Any of the roots, leaves, stems, etc. of Danshen can be used for the Danshen, and either raw or dried can be used.
상기 제1단계에서 60~80(v/v)%의 알코올 수용액을 사용할 수 있다. 또한 상기 알코올은 C1~C4 알코올일 수 있고, 메탄올, 에탄올, 프로판올, 이소프로판올, 부탄올 및 이소부탄올로 이루어진 군에서 선택될 수 있다. 가장 바람직하게는 에탄올을 사용할 수 있다. In the first step, an aqueous alcohol solution of 60 to 80 (v/v)% may be used. In addition, the alcohol may be a C1 to C4 alcohol, and may be selected from the group consisting of methanol, ethanol, propanol, isopropanol, butanol, and isobutanol. Most preferably, ethanol can be used.
상기 제1단계에서 단삼의 중량 대비 알코올 수용액이 5~20배 중량이 첨가되어 침지될 수 있다. In the first step, 5 to 20 times the weight of the alcohol solution compared to the weight of Danshen may be added and immersed.
상기 제2단계에서 저압 수증기 증류는 85~95℃에서 0.05~0.07Mpa, 3~5시간 동안 수행되는 것이 좋다. 이 때 특히 압력이 0.05Mpa보다 미만이거나 0.07Mpa를 초과할 경우, 항균 물질의 추출이 잘 되지 않을 수 있다. In the second step, the low-pressure steam distillation is preferably performed at 85 to 95 ° C. at 0.05 to 0.07 Mpa for 3 to 5 hours. At this time, in particular, when the pressure is less than 0.05Mpa or greater than 0.07Mpa, the extraction of the antibacterial substance may not be performed well.
본 발명은 상기 제조방법으로 제조된 단삼 추출물을 함유하는 항균 조성물에 관한 것이다. 상기 추출물은 솔잎 추출물 또는 단삼 추출물일 수 있다. The present invention relates to an antibacterial composition containing the Danshen extract prepared by the above production method. The extract may be pine needle extract or Danshen extract.
상기 단삼 추출물은 3.38㎍/㎖의 농도에서 50% 이상의 DPPH( 2,2-diphenyl-1-picrylhydrazyl) 라디컬 소거 활성이 있는 것일 수 있다. The Danshen extract may have a DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging activity of 50% or more at a concentration of 3.38 μg/ml.
상기 항균 조성물은 녹농균, 대장균 및 황색포도상구균으로 이루어진 군 중에서 선택되는 세균에 대한 항균 효능이 있는 것을 특징으로 한다.The antibacterial composition is characterized in that it has antibacterial efficacy against bacteria selected from the group consisting of Pseudomonas aeruginosa, Escherichia coli and Staphylococcus aureus.
또 다른 양태에서 상기 조성물은 알데하이드류인 노네날로 인해 유발되는 노인성 냄새의 소취 효능이 있는 것을 특징으로 한다. In another aspect, the composition is characterized in that it has an effect of deodorizing the senile odor caused by nonenal, an aldehyde.
상기 조성물은 손소독제 조성물일 수 있다. 상기 손소독제 조성물에는 에탄올 55~75 중량%, 2~4(w/v)% 과산화수소수 0.1~0.2 중량%, 증류수 10~30 중량% 및 식물 추출물 0.005~15 중량%가 포함될 수 있고, 상기 식물 추출물은 본 발명의 방법으로 제조한 것으로서, 10~106㎍/㎖인 것을 사용할 수 있지만, 항균 효과를 발휘할 수 있는 적절한 비용 면에서 상기 손소독제 조성물의 원료로 사용하는 추출물은 그 농도가 10~1000 ㎍/㎖인 것이 바람직하다. The composition may be a hand sanitizer composition. The hand sanitizer composition may include 55 to 75% by weight of ethanol, 0.1 to 0.2% by weight of 2 to 4 (w / v)% hydrogen peroxide, 10 to 30% by weight of distilled water, and 0.005 to 15% by weight of a plant extract, the plant The extract is prepared by the method of the present invention, and 10 to 10 6 μg / ml can be used, but the extract used as a raw material for the hand sanitizer composition has a concentration of 10 to 10 It is preferably 1000 μg/ml.
본 발명은 단삼 추출물을 함유하는 항균 조성물에 관한 것이다. 상기 단삼추출물은 알코올 수용액에 단삼을 첨가하여 침지한 후 저온 수증기 증류법을 이용하여 유용성 물질을 추출하는 방법을 통해 얻은 것이다. 상기 방법으로 얻은 단삼 추출물은 기존의 알코올 추출물이나 물 추출물, 초음파 처리 추출물 등에 비해 녹농균, 대장균 및 황색포도상구균에 대한 항균능이 우수하고, 노네날 알데하이드로 유발되는 냄새의 제거 효능이 뛰어난 것으로 확인된다. The present invention relates to an antibacterial composition containing a Danshen extract. The Danshen extract is obtained by adding and immersing Danshen in an aqueous alcohol solution, and then extracting useful substances using a low-temperature steam distillation method. It is confirmed that the Danshen extract obtained by the above method has excellent antibacterial activity against Pseudomonas aeruginosa, Escherichia coli and Staphylococcus aureus, and is excellent in removing odors caused by nonenalaldehyde, compared to conventional alcohol extracts, water extracts, ultrasonically treated extracts, etc.
이하 본 발명의 바람직한 실시예를 상세히 설명하기로 한다. 그러나, 본 발명은 여기서 설명되는 실시예에 한정되지 않고 다른 형태로 구체화될 수도 있다. 오히려, 여기서 소개되는 내용이 철저하고 완전해지도록, 당업자에게 본 발명의 사상을 충분히 전달하기 위해 제공하는 것이다. Hereinafter, preferred embodiments of the present invention will be described in detail. However, the present invention is not limited to the embodiments described herein and may be embodied in other forms. Rather, it is provided so that the present disclosure will be thorough and complete, and will fully convey the spirit of the invention to those skilled in the art.
<실시예 1. 단삼의 에탄올 수용액 침지 및 증류법에 따른 추출물 제조><Example 1. Preparation of extract by immersion and distillation of Danshen in ethanol solution>
하기 표 1의 조건으로, 건조 단삼 각 30g을 농도별 에탄올 수용액 300g에 침지한 후 이를 증류기에 넣고 80℃로 저압 증류하였다. 증류하여 얻은 응축물은 감압증류기를 이용하여 농축하였고, 실험에 사용할 시료들은 10, 100㎎/㎖의 농도로 소분하여 최종 실험 농도가 10, 100㎍/㎖이 될 수 있도록 DMSO(dimethyl sulfoxide)에 용해하여 -70℃에 얼려두었다. Under the conditions shown in Table 1 below, 30 g of each of dried ginseng was immersed in 300 g of an aqueous ethanol solution for each concentration, and then put into a distiller and distilled under low pressure at 80 ° C. The condensate obtained by distillation was concentrated using a vacuum distillation machine, and the samples to be used in the experiment were subdivided into concentrations of 10 and 100 mg/ml and mixed in dimethyl sulfoxide (DMSO) so that the final experimental concentrations were 10 and 100 μg/ml. It was dissolved and frozen at -70 °C.
<< 비교예comparative example 1. One. 침지용for dipping 에탄올 수용액 농도별, 증류 조건별 추출물 제조> Preparation of extracts by concentration of ethanol solution and distillation conditions>
하기 표 2의 조건으로 비교 대상 추출물을 제조하였다. Comparative extracts were prepared under the conditions shown in Table 2 below.
<비교예 2. 단삼의 물 추출물 제조><Comparative Example 2. Preparation of water extract of Danshen>
건조단삼 각 30g을 증류수 300g에 첨가한 후 80℃에서 48시간 동안 반응시킨 후 액상을 동결건조하여 단삼 물 추출물 분말을 얻었다. After each 30g of dried Dandelion was added to 300g of distilled water and reacted at 80 ° C for 48 hours, the liquid phase was lyophilized to obtain a water extract powder of Dansam.
<비교예 3. 단삼의 일반 에탄올 수용액 추출물 제조> <Comparative Example 3. Preparation of general ethanol aqueous solution extract of Danshen>
건조단삼 30g을 70%(v/v) 에탄올 수용액 300g에 첨가한 후 50℃에서 48시간 동안 반응시킨 후 액상을 감압농축하여 단삼 에탄올 수용액 추출물 농축물을 얻었다. After adding 30g of dry Danshen to 300g of 70% (v/v) aqueous ethanol solution, reacting at 50° C. for 48 hours, the liquid phase was concentrated under reduced pressure to obtain a concentrate of an aqueous solution of Danshen Danshen extract.
<비교예 4. 단삼의 저압 증류 추출물> <Comparative Example 4. Low-pressure distillation extract of Danshen>
실시예 1-1의 과정에서 에탄올 수용액 침지 조건만 생략하고 그대로 단삼을 증류기에 넣고 저압증류하여 에센셜 오일 상태의 추출물을 얻었다. In the process of Example 1-1, only the immersion condition in the aqueous ethanol solution was omitted, and Danshen was put into a still and distilled under low pressure to obtain an extract in an essential oil state.
<비교예 5. 단삼의 초음파 처리 추출물><Comparative Example 5. Danshen sonicated extract>
비교예 5-1. 에탄올 반응 후 초음파 처리Comparative Example 5-1. Sonication after ethanol reaction
실시예 1-1의 과정에서 단삼의 에탄올 수용액 침지 상태를 48시간 동안 유지한 후 초음파 분쇄기(LabaX, MA, USA)로 230 jule 30분간 처리하여 액상만을 수득하고 이를 감압증류하였고 최종 농축물을 얻었다. In the process of Example 1-1, Danshen was immersed in an ethanol aqueous solution for 48 hours, and then treated with an ultrasonic mill (LabaX, MA, USA) for 30 minutes at 230 jule to obtain only the liquid phase, which was distilled under reduced pressure to obtain the final concentrate. .
비교예 5-2. 증류수 반응 후 초음파 처리Comparative Example 5-2. Sonication after distilled water reaction
건조 단삼 30g을 증류수 300g에 첨가한 후 40℃에서 48시간 동안 반응시킨 후 초음파 분쇄기(LabaX, MA, USA)로 230 jule로 30분간 처리하여 액상만을 수득하고 이를 동결건조하여 분말을 얻었다. After adding 30 g of dried Danshen to 300 g of distilled water, reacted at 40 ° C. for 48 hours, and then treated with an ultrasonic grinder (LabaX, MA, USA) for 30 minutes at 230 jule to obtain only a liquid phase, which was lyophilized to obtain a powder.
<실험예 1. 항균 효능 확인><Experimental Example 1. Confirmation of antibacterial efficacy>
항균 효능을 확인하기 위해, 녹농균(Pseudomonas aeruginosa)은 NA(nutrient agar) plate, 대장균(Escherichia coli)은 LA(Luria-Bertani agar) plate, 황색포도상구균(Staphylococcus aureus)은 NA(nutrient agar) plate에 준비하여 배양하였다.To confirm the antibacterial efficacy, Pseudomonas aeruginosa was placed on a nutrient agar (NA) plate, Escherichia coli was placed on a Luria-Bertani agar (LA) plate, and Staphylococcus aureus was placed on a nutrient agar (NA) plate. prepared and cultured.
각 세균 plate의 중앙에 6㎜ paper disc를 올린 후, 10㎍/㎖로 희석된 각 추출물을 10㎍씩 점적하였다. After placing a 6 mm paper disc on the center of each bacterial plate, 10 μg of each extract diluted to 10 μg/ml was added dropwise.
이 후 37℃에서 3일간 배양한 후 항균환의 지름을 확인하였다. After incubation at 37 ° C. for 3 days, the diameter of the antibacterial ring was confirmed.
그 결과 실시예 1의 단삼 추출물의 항균 효능의 우수성을 확인할 수 있었다. As a result, it was confirmed that the antibacterial efficacy of the Danshen extract of Example 1 was excellent.
<실험예 2. 노네날 제거 효능 확인> <Experimental Example 2. Confirmation of Nonenal Removal Efficacy>
노인성 냄새의 주원인이 되는 알데하이드 물질인 노네날 제거 효과를 평가하기 위해 다음과 같이 실험을 진행하였다. 구체적으로, 25㎕의 트랜스-2-노네날(trans-2-nonenal)에 각 추출물을 475㎕ 처리해 준 뒤 3시간 교반시키고, 에탄올을 5ml 처리한 후 펠링솔루션I 150㎕, 펠링솔루션II 150 ㎕(동량 혼합)에 첨가하여 60℃의 조건으로 15분 반응시킨 뒤에 흡광도를 측정하였다. 상기 추출물들은 농도별로 실험하여 실험의 정확성을 확인하였다. In order to evaluate the effect of removing nonenal, an aldehyde substance that is the main cause of senile odor, the following experiment was conducted. Specifically, 475 μl of each extract was treated with 25 μl of trans-2-nonenal, stirred for 3 hours, treated with 5 ml of ethanol, and then 150 μl of Pelling Solution I and 150 μl of Pelling Solution II (Equal amount mixing) and reacted at 60 ° C for 15 minutes, and then the absorbance was measured. The extracts were tested for each concentration to confirm the accuracy of the experiment.
표 4는 트랜스-2-노네날에 각 추출물을 처리하여 펠링반응을 한 결과이다. 각 결과는 오직 트랜스-2-노네날만 처리하여 펠링반응을 한 결과 대비 흡광 정도를 계산하여 트랜스-2-노네날의 농도(trans-2-nonenal activity, %)를 나타냈다. 이에 표 4의 수치값이 적을수록 노네날로 인해 유발되는 냄새가 줄어들었음을 확인할 수 있다. Table 4 shows the results of the Pelling reaction by treating each extract with trans-2-nonenal. Each result showed the concentration of trans-2-nonenal (trans-2-nonenal activity, %) by calculating the degree of absorption compared to the result of the Felling reaction by treating only trans-2-nonenal. Accordingly, it can be confirmed that the smaller the numerical value in Table 4, the less the odor caused by nonenal.
측정 결과, 표 4에 나타난 바와 같이, 실시예 1의 추출물을 처리했을 때 트랜스-2-노네날의 농도가 줄어든 것으로 나타나, 노인성 냄새를 효과적으로 제거할 수 있음을 확인하였다.As a result of the measurement, as shown in Table 4, when the extract of Example 1 was treated, the concentration of trans-2-nonenal was reduced, confirming that the senile odor could be effectively removed.
<실험예 3. DPPH 라디컬 소거능 확인><Experimental Example 3. Confirmation of DPPH radical scavenging ability>
DPPH(1,1-Diphenyl-2-picrylhydrazyl) 라디칼 소거능은 Cho 등(2011)의 방법을 이용하여 측정하였다. 먼저 동결건조된 시료를 DMSO(dimethyl sulfoxide)에 녹여 100,000㎍/㎖으로 stock solution을 제작한 후, 최종 농도에 맞추어 에탄올에 용해하여 사용하였다. 시료 10㎕과 150μM DPPH 용액 190㎕를 혼합한 뒤, 30분 동안 차광조건에서 반응한 후 Microplate reader(Tecan, Switzerland)의 517 nm 조건에서 흡광도 값을 측정하였다. 실험에 사용한 시료의 최종 농도는 1~100㎍/㎖이 되게 하였고, 양성대조군으로는 퀘르세틴(Quercetin 10 μM, QC10)을 사용하였다. 결과값은 IC50으로 나타내었고, 이를 표 5에 기재하였다. DPPH (1,1-Diphenyl-2-picrylhydrazyl) radical scavenging ability was measured using the method of Cho et al. (2011). First, the lyophilized sample was dissolved in dimethyl sulfoxide (DMSO) to prepare a stock solution at 100,000 μg/ml, and then dissolved in ethanol according to the final concentration. After mixing 10 μl of the sample and 190 μl of a 150 μM DPPH solution, reacting under light-shielding conditions for 30 minutes, the absorbance value was measured at 517 nm using a microplate reader (Tecan, Switzerland). The final concentration of the sample used in the experiment was 1 to 100 μg/ml, and quercetin (Quercetin 10 μM, QC10) was used as a positive control. The resultant values were expressed as IC 50 , which are listed in Table 5.
그 결과 실시예 1 추출물의 항산화 효능이 매우 우수함을 확인할 수 있다. As a result, it can be confirmed that the antioxidant efficacy of the extract of Example 1 is very good.
<< 제형예formulation example 1. One. 손소독제Hand sanitizer 제조> manufacturing>
순도 99% 에탄올 55g, 3(w/v)% 과산화수소수 0.2g, 1000㎍/㎖ 실시예 1-1 솔잎 추출물 10g을 혼합하고, 증류수를 넣어 총 100g이 되게 하고 분무기에 담아 스프레이용 손소독제 조성물을 제조하였다. Mix 55 g of pure 99% ethanol, 0.2 g of 3 (w/v)% hydrogen peroxide, and 10 g of 1000 μg/ml Example 1-1 pine needle extract, add distilled water to make a total of 100 g, and put in a sprayer to make a hand sanitizer composition for spraying was manufactured.
<실험예 4. 손소독제 조성물의 항균 효과><Experimental Example 4. Antibacterial effect of hand sanitizer composition>
제형예 1의 손소독제 제조 후, 녹농균(Pseudomonas aeruginosa)이 배양된 NA(nutrient agar) plate, 대장균(Escherichia coli)이 배양된 LA(Luria-Bertani agar) plate, 황색포도상구균(Staphylococcus aureus)이 배양된 NA(nutrient agar) plate에 상기 손소독제 조성물을 스프레이 하고 24시간 후 세균을 스크레치하여 새로운 agar plate에 옮겨담았다. After preparing the hand sanitizer of Formulation Example 1, Pseudomonas aeruginosa ( Pseudomonas aeruginosa ) cultured NA (nutrient agar) plate, Escherichia coli ( Escherichia coli ) cultured LA (Luria-Bertani agar) plate, Staphylococcus aureus ( Staphylococcus aureus ) cultured The hand sanitizer composition was sprayed on a nutrient agar (NA) plate, and after 24 hours, the bacteria were scratched and transferred to a new agar plate.
그 결과, 모든 plate에서 세균이 자라지 않았고, 이를 통해 손소독제 조성물의 뛰어난 항균 효과를 알 수 있었다. 상기 손소독제 조성물은 에탄올의 함량이 기존 소독제 조성물보다 적지만 본 발명에서 제조된 솔잎 추출물이 포함되어 항균효과가 증강된 것임을 확인할 수 있다. As a result, bacteria did not grow on all plates, and through this, the excellent antibacterial effect of the hand sanitizer composition was found. It can be seen that the hand sanitizer composition has a lower ethanol content than the conventional disinfectant composition, but contains the pine needle extract prepared in the present invention, thereby enhancing the antibacterial effect.
Claims (7)
(제2단계) 알코올 수용액에 침지된 식물을 저압 수증기 증류법으로 증류하는 단계;
(제3단계) 증류된 증기를 수집하고 응축하여 액상을 얻는 단계; 및,
(제4단계) 상기 액상을 감압증류하여 농축물을 얻는 단계;
를 포함하는 것을 특징으로 하는 단삼 추출물을 함유하는 항균용 조성물의 제조방법.(First step) immersing Danshen in an aqueous alcohol solution;
(Second step) distilling the plant immersed in an aqueous alcohol solution by low pressure steam distillation;
(Third Step) Collecting and condensing the distilled vapor to obtain a liquid phase; and,
(Fourth step) distilling the liquid phase under reduced pressure to obtain a concentrate;
Method for producing an antibacterial composition containing a Danshen extract, characterized in that it comprises a.
상기 제1단계에서 60~80(v/v)%의 알코올 수용액을 사용하는 것을 특징으로 하는 항균용 조성물의 제조방법.According to claim 1,
Method for producing an antimicrobial composition, characterized in that using an aqueous solution of 60 ~ 80 (v / v)% alcohol in the first step.
상기 조성물은 녹농균, 대장균 및 황색포도상구균으로 이루어진 군 중에서 선택되는 세균에 대한 항균 효능이 있는 것을 특징으로 하는 항균 조성물.According to claim 3,
The composition is an antibacterial composition, characterized in that there is an antibacterial effect against bacteria selected from the group consisting of Pseudomonas aeruginosa, Escherichia coli and Staphylococcus aureus.
상기 조성물은 알데하이드류인 노네날로 인해 유발되는 노인성 냄새의 소취 효능이 있는 것을 특징으로 하는 항균 조성물.According to claim 3,
The antibacterial composition, characterized in that the composition has a deodorizing effect of the senile odor caused by nonenal, an aldehyde.
에탄올 55~75 중량%, 2~4(w/v)% 과산화수소수 0.1~0.2 중량%, 증류수 10~30 중량% 및 제3항의 조성물 0.005~15 중량%가 포함된 것을 특징으로 하는 손소독제 조성물.According to claim 6,
Hand sanitizer composition comprising 55 to 75% by weight of ethanol, 0.1 to 0.2% by weight of 2 to 4 (w / v)% hydrogen peroxide, 10 to 30% by weight of distilled water and 0.005 to 15% by weight of the composition of claim 3 .
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| KR101613294B1 (en) * | 2015-07-02 | 2016-04-19 | 이엠생활환경 주식회사 | An antibiotic deodorization composition comprising EM fermentation metabolites and salvia miltiorrhiza extracts |
| KR20170021573A (en) * | 2015-08-18 | 2017-02-28 | 허진희 | External use sanitizers containing hinoki cypress leaf extract and lemon leaf extract and manufacturing method of the same |
| KR102120835B1 (en) * | 2018-12-24 | 2020-06-09 | 재단법인 경남한방항노화연구원 | Natural deodorant composition using polyphenol |
| KR102269881B1 (en) | 2018-03-14 | 2021-06-29 | 정연옥 | Composition with antimicrobial or antifungal activity comprising dryopteris crassirhizoma nakai extract, salvia miltiorrhiza bunge extract and scutellaria baicalensis extract and method for preaparing the same |
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| KR101613294B1 (en) * | 2015-07-02 | 2016-04-19 | 이엠생활환경 주식회사 | An antibiotic deodorization composition comprising EM fermentation metabolites and salvia miltiorrhiza extracts |
| KR20170021573A (en) * | 2015-08-18 | 2017-02-28 | 허진희 | External use sanitizers containing hinoki cypress leaf extract and lemon leaf extract and manufacturing method of the same |
| KR102269881B1 (en) | 2018-03-14 | 2021-06-29 | 정연옥 | Composition with antimicrobial or antifungal activity comprising dryopteris crassirhizoma nakai extract, salvia miltiorrhiza bunge extract and scutellaria baicalensis extract and method for preaparing the same |
| KR102120835B1 (en) * | 2018-12-24 | 2020-06-09 | 재단법인 경남한방항노화연구원 | Natural deodorant composition using polyphenol |
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