KR20230078978A - Developing nutrient solution for growing coastal glehnia in plant factories with artificial lighting - Google Patents

Abstract

The present invention relates to a culture medium exclusively for the cultivation of sea breeze in a plant factory using artificial lighting,
In one aspect of the present invention, the composition of the culture solution for promoting the growth of sea breeze (scientific name: Glehnia littoralis ) is similar to the ratio of inorganic ions preferred by sea breeze and adjusted to a specific electrical conductivity level, so that the conventional Hoagland nutrient solution In contrast, it has the effect of inducing relatively excellent growth promotion of sea breeze.

Description

Development of exclusive culture solution for cultivation of sea breeze in plant factories using artificial lighting {Developing nutrient solution for growing coastal glehnia in plant factories with artificial lighting}

The present invention relates to a culture solution exclusively for the cultivation of sea breeze in a plant factory using artificial lighting.

Plant factories can produce high-quality crops in large quantities throughout the year through precise environmental control such as temperature, humidity, light (light), carbon dioxide, and nutrients, but have high initial investment costs and maintenance costs. In recent years, the need for securing raw materials and maintaining quality of plant resources used in health functional foods or pharmaceutical industries has increased, and interest in growing medicinal crops through environmental control in plant factories has increased.

Gaetbangpung ( Glehnia littoralis Fr. schmidt ex Miq. ) is a perennial herb belonging to the family Umbrella and is distributed in coastal sand dunes in Korea. It is a medicinal crop with various medicinal effects such as antipyretic, expectorant, anti-inflammatory, antioxidant, etc. Glehnia: A Systematic Review, PMID: 31915441).

Cultivation methods for most medicinal crops, including Gaetbangpung, are not known, and moreover, cultivation techniques for mass production of medicinal crops in plant factories have not been developed. In order to establish a system for mass production of medicinal crops using plant factories, it is necessary to develop optimal cultivation technologies for the relevant crops.

For the production of medicinal crops in plant factories, hydroponic cultivation is required first, and since each crop has a different preference for a specific nutrient, it is necessary to develop a culture medium exclusively for the target crop. In addition, since a low culture medium concentration induces poor growth, and a high culture medium concentration restricts nutrient absorption due to osmotic stress, selection of an appropriate concentration is also an important factor.

While the present applicant was trying to develop a culture medium exclusively for the cultivation of sea breeze in a plant factory using artificial light, the composition of the culture solution composition for promoting the growth of sea breeze provided in one aspect of the present invention is The present invention was completed by proving that the ratio of preferred inorganic ions was similar to that of the conventional Hoagland nutrient solution, and that relatively excellent growth promotion of sea breeze could be induced.

Evid Based Complement Alternat Med. 2019; 2019: 1253493, 33 pages, Ethnopharmacology, Phytochemistry, and Pharmacology of the Genus Glehnia: A Systematic Review, PMID: 31915441.

An object of one aspect of the present invention is,

The composition of the culture solution composition is similar to the ratio of inorganic ions preferred by the sea breeze and is adjusted to a specific electrical conductivity level, compared to the conventional Hoagland nutrient solution. ) To provide a culture composition for promoting growth.

Another object of the present invention is,

It is to provide a method of cultivating a seabreak (scientific name: Glehnia littoralis ) plant using the above-mentioned seabreak (scientific name: Glehnia littoralis ) growth-promoting culture solution composition.

In order to achieve the above purpose,

One aspect of the present invention,

Culture medium A containing 62 to 70 parts by weight of calcium nitrate (Ca(NO ₃ ) ₂ .4H ₂ O) and 0.5 to 1.7 parts by weight of iron (Fe-EDTA) based on 42.925 parts by weight of potassium nitrate (KNO ₃ ); and

Based on 42.925 parts by weight of potassium nitrate (KNO ₃ ), 3.0 to 6.0 parts by weight of ammonium phosphate (NH ₄ H ₂ PO ₄ ), 4.0 to 7.0 parts by weight of potassium phosphate (KH ₂ PO ₄ ), magnesium sulfate (MgSO ₄ ) 7H ₂ O) 30 to 35 parts by weight, copper sulfate (CuSO ₄ 5H ₂ O) 0.001 to 0.03 parts by weight, boron (H ₃ BO ₃ ) 0.05 to 0.35 parts by weight, manganese sulfate (MnSO ₄ 5H ₂ O) 0.1 to Culture medium B containing 0.4 parts by weight, zinc sulfate (ZnSO ₄ 7H ₂ O) 0.01 to 0.3 parts by weight, sodium molybdate (Na ₂ MoO ₄ 2H ₂ O) 0.001 to 0.03 parts by weight; , A sea breeze (scientific name: Glehnia littoralis ) provides a culture composition for promoting growth.

In addition, another aspect of the present invention,

Provided is a method of cultivating a seabreak (scientific name: Glehnia littoralis ) plant using the growth-promoting culture composition of the seabreak wind (scientific name: Glehnia littoralis ).

In one aspect of the present invention, the composition of the culture solution for promoting the growth of sea breeze (scientific name: Glehnia littoralis ) is similar to the ratio of inorganic ions preferred by sea breeze and adjusted to a specific electrical conductivity level, so that the conventional Hoagland nutrient solution In contrast, it has the effect of inducing relatively excellent growth promotion of sea breeze.

), H2(

), N1(

) 그리고 N2(

)의 기존 설정한 pH 5.8에서 변화되는 pH 값을 절대값으로 표현한 결과로, 검은 점선은 H1과 H2, 노란 점선은 N1과 N2의 18주간 주 3회 측정된 pH 변화 절대값의 평균을 표현한 것이다.Figure 1 shows the cations K , Ca, Mg and anions N, P, It is a drawing showing the result of comparing the ratio of S.
Figure 2 shows leaf live weight (Fig. 2. A), leaf dry weight (Fig. 2. B), leaf area (Fig. 2. C) and above-ground area (Fig. 2. D) is the result.
Figure 3 is the average leaf live weight (Fig. 3. A), leaf dry weight (Fig. 3. B), leaf area (Fig. 3. C) and aboveground area (Fig. 3 .D) is the result.
Figure 4 accumulates the results of the 1st, 2nd and 3rd harvests, and leaf live weight (Fig. 4. A), leaf dry weight (Fig. 4. B), leaf area (Fig. 4. C) and above-ground area (Fig. 4. D ) is the result showing.
Figure 5 shows H1 during the 18-week cultivation period (

), H2(

), N1(

) and N2(

) is the result of expressing the pH value changing from the previously set pH 5.8 as an absolute value, the black dotted line represents the average of the absolute value of the pH change measured three times a week for 18 weeks in H1 and H2, and the yellow dotted line is N1 and N2 .

Hereinafter, the present invention will be described in detail.

Meanwhile, the embodiments of the present invention may be modified in various forms, and the scope of the present invention is not limited to the embodiments described below. In addition, the embodiments of the present invention are provided to more completely explain the present invention to those skilled in the art.

Furthermore, "include" a certain component throughout the specification means that other components may be further included without excluding other components unless otherwise stated.

One aspect of the present invention,

Culture medium A containing 62 to 70 parts by weight of calcium nitrate (Ca(NO ₃ ) ₂ .4H ₂ O) and 0.5 to 1.7 parts by weight of iron (Fe-EDTA) based on 42.925 parts by weight of potassium nitrate (KNO ₃ ); and

Based on 42.925 parts by weight of potassium nitrate (KNO ₃ ), 3.0 to 6.0 parts by weight of ammonium phosphate (NH ₄ H ₂ PO ₄ ), 4.0 to 7.0 parts by weight of potassium phosphate (KH ₂ PO ₄ ), magnesium sulfate (MgSO ₄ ) 7H ₂ O) 30 to 35 parts by weight, copper sulfate (CuSO ₄ 5H ₂ O) 0.001 to 0.03 parts by weight, boron (H ₃ BO ₃ ) 0.05 to 0.35 parts by weight, manganese sulfate (MnSO ₄ 5H ₂ O) 0.1 to Culture medium B containing 0.4 parts by weight, zinc sulfate (ZnSO ₄ 7H ₂ O) 0.01 to 0.3 parts by weight, sodium molybdate (Na ₂ MoO ₄ 2H ₂ O) 0.001 to 0.03 parts by weight; , A sea breeze (scientific name: Glehnia littoralis ) provides a culture composition for promoting growth.

more preferably,

The culture composition,

Culture medium A containing 64 to 68 parts by weight of calcium nitrate (Ca(NO ₃ ) ₂ .4H ₂ O) and 0.7 to 1.5 parts by weight of iron (Fe-EDTA) based on 42.925 parts by weight of potassium nitrate (KNO ₃ ); and

Based on 42.925 parts by weight of potassium nitrate (KNO ₃ ), 3.5 to 5.5 parts by weight of ammonium phosphate (NH ₄ H ₂ PO ₄ ), 4.5 to 6.5 parts by weight of potassium phosphate (KH ₂ PO ₄ ), magnesium sulfate (MgSO ₄ . 7H ₂ O) 31 to 34 parts by weight, copper sulfate (CuSO ₄ 5H ₂ O) 0.005 to 0.025 parts by weight, boron (H ₃ BO ₃ ) 0.1 to 0.3 parts by weight, manganese sulfate (MnSO ₄ 5H ₂ O) 0.15 to 0.15 parts by weight 0.35 parts by weight, zinc sulfate (ZnSO ₄ 7H ₂ O) 0.05 to 0.25 parts by weight, sodium molybdate (Na ₂ MoO ₄ 2H ₂ O) 0.005 to 0.025 parts by weight of culture medium B containing; may include.

Even more preferably,

The culture composition,

Culture medium A containing 65 to 67 parts by weight of calcium nitrate (Ca(NO ₃ ) ₂ .4H ₂ O) and 0.9 to 1.3 parts by weight of iron (Fe-EDTA) based on 42.925 parts by weight of potassium nitrate (KNO ₃ ); and

Based on 42.925 parts by weight of potassium nitrate (KNO ₃ ), 4.0 to 5.0 parts by weight of ammonium phosphate (NH ₄ H ₂ PO ₄ ), 5.0 to 6.0 parts by weight of potassium phosphate (KH ₂ PO ₄ ), magnesium sulfate (MgSO ₄ ) 7H ₂ O) 32 to 33 parts by weight, copper sulfate (CuSO ₄ 5H ₂ O) 0.01 to 0.02 parts by weight, boron (H ₃ BO ₃ ) 0.15 to 0.25 parts by weight, manganese sulfate (MnSO ₄ 5H ₂ O) 0.2 to 0.25 parts by weight 0.3 parts by weight, zinc sulfate (ZnSO ₄ · 7H ₂ O) 0.1 to 0.2 parts by weight, sodium molybdate (Na ₂ MoO ₄ · 2H ₂ O) 0.01 to 0.02 parts by weight of culture medium B containing; may include.

most preferably,

The culture composition,

Culture medium A containing 66.08 parts by weight of calcium nitrate (Ca(NO ₃ ) ₂ .4H ₂ O) and 1.15 parts by weight of iron (Fe-EDTA) based on 42.925 parts by weight of potassium nitrate (KNO ₃ ); and

Based on potassium nitrate (KNO ₃ ) 42.925 parts by weight, ammonium phosphate monobasic (NH ₄ H ₂ PO ₄ ) 4.6 parts by weight, potassium phosphate (KH ₂ PO ₄ ) 5.44 parts by weight, magnesium sulfate (MgSO ₄ 7H ₂ O) 32.472 parts by weight, copper sulfate (CuSO ₄ 5H ₂ O) 0.01768 parts by weight, boron (H ₃ BO ₃ ) 0.20009 parts by weight, manganese sulfate (MnSO ₄ 5H ₂ O) 0.24492 parts by weight, zinc sulfate (ZnSO ₄ 7H ₂ O) 0.15391 parts by weight, culture medium B containing 0.01261 parts by weight of sodium molybdate (Na ₂ MoO ₄ 2H ₂ O);

If each component in the most preferred culture medium composition is expressed in g per 1L volume,

The culture composition,

Culture medium A containing 66.08 g of Ca(NO ₃ ) ₂ ·4H ₂ O, 42.925 g of KNO ₃ , and 1.15 g of Fe-EDTA per 1 L of volume; and

KNO ₃ 42.925g, NH ₄ H ₂ PO ₄ 4.6g, KH ₂ PO ₄ 5.44g, MgSO ₄ 7H ₂ O 32.472g, CuSO ₄ 5H ₂ O 0.01768g, H ₃ BO ₃ 0.20009g, MnSO per 1L volume ₄ · 5H ₂ O 0.24492g, ZnSO ₄ · 7H ₂ O 0.15391g and Na ₂ MoO ₄ · 2H ₂ O 0.01261g containing culture medium B; may include.

When indicated as a culture medium in which the culture medium A and the culture medium B are mixed,

The culture composition,

Ca(NO ₃ ) ₂ 4H ₂ O 66.08mg, KNO ₃ 85.85mg, Fe-EDTA 1.15mg, NH ₄ H ₂ PO ₄ 4.6mg, KH ₂ PO ₄ 5.44mg, MgSO ₄ 7H ₂ O 32.472 per 1L volume mg, CuSO ₄ 5H ₂ O 0.01768 mg, H ₃ BO ₃ 0.20009 mg, MnSO ₄ 5H ₂ O 0.24492 mg, ZnSO ₄ 7H ₂ O 0.15391 mg, and Na ₂ MoO ₄ 2H ₂ O 0.01261 mg. there is.

Next, the electrical conductivity (EC, Electrical Conductivity) of the growth-promoting culture solution composition for sea breeze (scientific name: Glehnia littoralis ) provided in one aspect of the present invention may be 0.5 to 5.5 dS·m ^-1 . It may be more preferably from 0.5 to 1.5 dS·m ^-1 , even more preferably from 0.8 to 1.2 dS·m -1 , more preferably from 0.9 to 1.1 dS·m ^-1 , and most preferably from 0.9 to 1.1 dS·m ^-1 . It may be preferably 1.0 dS·m ^-1 .

In the experimental example of the present invention,

When indicated as a culture medium in which culture medium A and culture medium B are mixed,

Ca(NO ₃ ) ₂ 4H ₂ O 66.08mg, KNO ₃ 85.85mg, Fe-EDTA 1.15mg, NH ₄ H ₂ PO ₄ 4.6mg, KH ₂ PO ₄ 5.44mg, MgSO ₄ 7H ₂ O 32.472 per 1L volume mg, CuSO ₄ 5H ₂ O 0.01768 mg, H ₃ BO ₃ 0.20009 mg, MnSO ₄ 5H ₂ O 0.24492 mg, ZnSO ₄ 7H ₂ O 0.15391 mg and Na ₂ MoO ₄ 2H ₂ O 0.01261 mg; At the same time, the electrical conductivity EC (dS / m) is 1.0 dS · m ^-1 condition (level), N1 proves that the most optimal sea breeze-only culture medium (see Figs. 2 to 5 of Experimental Example 2).

Another aspect of the present invention is,

Provides a method of growing a sea breeze (scientific name: Glehnia littoralis ) plant using the above culture composition.

At this time, the cultivation method of the sea breeze plant,

A planting step of planting seedlings of sea breeze plants in hydroponic cultivation facilities;

The sea breeze plant is supplied with carbon dioxide in the range of 400 to 1000 ppm under a light intensity in the range of 100 to 500 μmol m ^-2 s ^-1 , maintaining a temperature of 15 to 25 ° C, and growing by applying the culture solution composition growth stage; and

It may include; harvesting the sea breeze plants that have undergone the growth step.

more preferably,

In the growth step, the light intensity may be in the range of 200 to 400 μmol m ⁻² s ⁻¹ , the carbon dioxide in the range of 400 to 700 ppm, and the temperature in the range of 18 to 22 ° C.

In the growth step, the light intensity may be in the range of 250 to 350 μmol·m ^-2 ·s ^-1 , carbon dioxide may be in the range of 500 to 600 ppm, and the temperature may be in the range of 19 to 21 °C , but is not particularly limited thereto.

The planting step is a step of planting seedlings of sea breeze plants in hydroponic cultivation facilities. The facility for hydroponic cultivation may be a facility for growing indoor crops called a plant factory. The plant factory can grow plants efficiently indoors by adjusting the temperature, humidity, nutrient components of the medium, and the like, such as light source and carbon dioxide in the facility. The hydroponic cultivation facility may be a plant factory thin film hydroponic (NFT: Nutrient film technique) or DFT: Deep flow technique (DFT) system, but is not particularly limited thereto. Since the thin film hydroponic system has a simple structure and is lightweight, high work efficiency is high because it is easy to grow crops. In addition, since the nutrient solution flows according to the inclination of the bed, it is easy to supply oxygen to the roots and has good responsiveness in controlling the environment of the root zone. The bile hydroponic system is a method in which the roots are contained in a deep culture medium, and the amount of culture medium in the bed is large, so there is little variation in the root environment, and the culture medium concentration, nutrient ion balance, and pH control are maintained uniformly, and the culture medium can be supplied. .

The growth step is a step of growing a sea breeze plant while adjusting the light intensity, carbon dioxide, ambient temperature, etc. by applying the culture medium composition for promoting growth of sea breeze (scientific name: Glehnia littoralis ) provided in one aspect of the present invention.

In the growth step, the light intensity may be in the range of 100 to 500 μmol m ^-2 s ^-1 . When the light intensity is maintained in the range, the growth of sea breeze plants can be promoted, and the light intensity is 100 μmol m A low light intensity that is less than ^-2 · s ^-1 inhibits photosynthesis and may result in overgrowth or insufficient growth of sea breeze plants, and an excessive light intensity of more than 500 μmol · m ^-2 · s ^-1 The amount of light wastes electrical energy and partially destroys the chlorophyll of the plant or inactivates conditions in the body to inhibit photosynthesis, and rather causes a problem in that plant growth cannot be induced excellently.

In addition, the carbon dioxide in the growth step may be in the range of 400 to 1000 ppm, but the carbon dioxide in the atmosphere is 400 ppm, and if the carbon dioxide is less than 400 ppm in the facility, it becomes a limiting factor in photosynthesis, so that the sea breeze plants cannot grow sufficiently When supplied in excess of 1000 ppm, the photosynthetic efficiency of plants may decrease compared to the supplied carbon dioxide, and carbon dioxide may be wasted.

Furthermore, in the growth step, the cultivation temperature can be maintained at 15 to 25 ° C., and when the temperature range is maintained, the growth of windbreak plants can be efficiently induced. If the cultivation temperature is less than 15 ° C, low-temperature stress becomes a limiting factor in photosynthesis, and the growth of sea breeze plants may be slowed down. can do.

In order to efficiently grow seawater plants in the hydroponic cultivation facility, it is preferable to cultivate them using a nutrient solution exclusively for seawater plants. It is possible to increase the cultivation efficiency of functional crops that can be used as pharmaceutical-based raw materials by maximizing the content of functional ingredients contained therein.

The culture solution used in the method of cultivating a seabreak plant according to one aspect of the present invention is potassium nitrate (KNO ₃ ) based on 42.925 parts by weight, calcium nitrate (Ca (NO ₃ ) ₂ 4H ₂ O) 62 to 70 parts by weight A culture medium A containing 0.5 to 1.7 parts by weight of iron (Fe-EDTA); and

Based on 42.925 parts by weight of potassium nitrate (KNO ₃ ), 3.0 to 6.0 parts by weight of ammonium phosphate (NH ₄ H ₂ PO ₄ ), 4.0 to 7.0 parts by weight of potassium phosphate (KH ₂ PO ₄ ), magnesium sulfate (MgSO ₄ ) 7H ₂ O) 30 to 35 parts by weight, copper sulfate (CuSO ₄ 5H ₂ O) 0.001 to 0.03 parts by weight, boron (H ₃ BO ₃ ) 0.05 to 0.35 parts by weight, manganese sulfate (MnSO ₄ 5H ₂ O) 0.1 to 0.4 parts by weight, zinc sulfate (ZnSO ₄ 7H ₂ O) 0.01 to 0.3 parts by weight, sodium molybdate (Na ₂ MoO ₄ 2H ₂ O) 0.001 to 0.03 parts by weight of culture medium B containing; : Glehnia littoralis ) It may be a culture medium composition for promoting growth.

more preferably,

The culture composition,

Culture medium A containing 64 to 68 parts by weight of calcium nitrate (Ca(NO ₃ ) ₂ .4H ₂ O) and 0.7 to 1.5 parts by weight of iron (Fe-EDTA) based on 42.925 parts by weight of potassium nitrate (KNO ₃ ); and

Based on 42.925 parts by weight of potassium nitrate (KNO ₃ ), 3.5 to 5.5 parts by weight of ammonium phosphate (NH ₄ H ₂ PO ₄ ), 4.5 to 6.5 parts by weight of potassium phosphate (KH ₂ PO ₄ ), magnesium sulfate (MgSO ₄ . 7H ₂ O) 31 to 34 parts by weight, copper sulfate (CuSO ₄ 5H ₂ O) 0.005 to 0.025 parts by weight, boron (H ₃ BO ₃ ) 0.1 to 0.3 parts by weight, manganese sulfate (MnSO ₄ 5H ₂ O) 0.15 to 0.15 parts by weight 0.35 parts by weight, zinc sulfate (ZnSO ₄ 7H ₂ O) 0.05 to 0.25 parts by weight, sodium molybdate (Na ₂ MoO ₄ 2H ₂ O) 0.005 to 0.025 parts by weight of culture medium B containing; may include.

Even more preferably,

The culture composition,

Culture medium A containing 65 to 67 parts by weight of calcium nitrate (Ca(NO ₃ ) ₂ .4H ₂ O) and 0.9 to 1.3 parts by weight of iron (Fe-EDTA) based on 42.925 parts by weight of potassium nitrate (KNO ₃ ); and

Based on 42.925 parts by weight of potassium nitrate (KNO ₃ ), 4.0 to 5.0 parts by weight of ammonium phosphate (NH ₄ H ₂ PO ₄ ), 5.0 to 6.0 parts by weight of potassium phosphate (KH ₂ PO ₄ ), magnesium sulfate (MgSO ₄ ) 7H ₂ O) 32 to 33 parts by weight, copper sulfate (CuSO ₄ 5H ₂ O) 0.01 to 0.02 parts by weight, boron (H ₃ BO ₃ ) 0.15 to 0.25 parts by weight, manganese sulfate (MnSO ₄ 5H ₂ O) 0.2 to 0.25 parts by weight 0.3 parts by weight, zinc sulfate (ZnSO ₄ · 7H ₂ O) 0.1 to 0.2 parts by weight, sodium molybdate (Na ₂ MoO ₄ · 2H ₂ O) 0.01 to 0.02 parts by weight of culture medium B containing; may include.

most preferably,

The culture composition,

Culture medium A containing 66.08 parts by weight of calcium nitrate (Ca(NO ₃ ) ₂ .4H ₂ O) and 1.15 parts by weight of iron (Fe-EDTA) based on 42.925 parts by weight of potassium nitrate (KNO ₃ ); and

Based on potassium nitrate (KNO ₃ ) 42.925 parts by weight, ammonium phosphate monobasic (NH ₄ H ₂ PO ₄ ) 4.6 parts by weight, potassium phosphate (KH ₂ PO ₄ ) 5.44 parts by weight, magnesium sulfate (MgSO ₄ 7H ₂ O) 32.472 parts by weight, copper sulfate (CuSO ₄ 5H ₂ O) 0.01768 parts by weight, boron (H ₃ BO ₃ ) 0.20009 parts by weight, manganese sulfate (MnSO ₄ 5H ₂ O) 0.24492 parts by weight, zinc sulfate (ZnSO ₄ 7H ₂ O) 0.15391 parts by weight, culture medium B containing 0.01261 parts by weight of sodium molybdate (Na ₂ MoO ₄ 2H ₂ O);

If each component in the most preferred culture medium composition is expressed in g per 1L volume,

The culture composition,

Culture medium A containing 66.08 g of Ca(NO ₃ ) ₂ ·4H ₂ O, 42.925 g of KNO ₃ , and 1.15 g of Fe-EDTA per 1 L of volume; and

KNO ₃ 42.925g, NH ₄ H ₂ PO ₄ 4.6g, KH ₂ PO ₄ 5.44g, MgSO ₄ 7H ₂ O 32.472g, CuSO ₄ 5H ₂ O 0.01768g, H ₃ BO ₃ 0.20009g, MnSO per 1L volume ₄ · 5H ₂ O 0.24492g, ZnSO ₄ · 7H ₂ O 0.15391g and Na ₂ MoO ₄ · 2H ₂ O 0.01261g containing culture medium B; may include.

The culture medium A and the culture medium B contained in the culture medium composition for promoting the growth of sea breeze can be prepared before use and stored in a refrigerator, mixing and diluting if necessary.

When indicated as a culture medium in which the culture medium A and the culture medium B are mixed,

The culture composition,

Ca(NO ₃ ) ₂ 4H ₂ O 66.08mg, KNO ₃ 85.85mg, Fe-EDTA 1.15mg, NH ₄ H ₂ PO ₄ 4.6mg, KH ₂ PO ₄ 5.44mg, MgSO ₄ 7H ₂ O 32.472 per 1L volume mg, CuSO ₄ 5H ₂ O 0.01768 mg, H ₃ BO ₃ 0.20009 mg, MnSO ₄ 5H ₂ O 0.24492 mg, ZnSO ₄ 7H ₂ O 0.15391 mg, and Na ₂ MoO ₄ 2H ₂ O 0.01261 mg. there is.

In the case of applying the composition of the growth-promoting culture solution of the sea breeze (scientific name: Glehnia littoralis ) as described above, while efficiently growing the sea breeze plants in the hydroponic cultivation facility, the content of components that become indicator components when used as a pharmaceutical-based raw material can be maximized, and it is especially advantageous for windbreak cultivation.

The sea breeze (scientific name: Glehnia littoralis ) The electrical conductivity (EC, Electrical Conductivity) of the culture medium composition for promoting growth may be 0.5 to 5.5 dS·m ^-1 . It may be more preferably from 0.5 to 1.5 dS·m ^-1 , even more preferably from 0.8 to 1.2 dS·m -1 , more preferably from 0.9 to 1.1 dS·m ^-1 , and most preferably from 0.9 to 1.1 dS·m ^-1 . It may be preferably 1.0 dS·m ^-1 .

If the electrical conductivity of the growth-promoting culture medium composition of the sea breeze (scientific name: Glehnia littoralis ) is less than 0.5 dS m ^-1 , it does not provide sufficient inorganic nutrients to the plant, so the growth of the sea breeze plant is not smooth, so the number of leaves or The area may not be sufficient and mineral deficiency may appear in the plant. In the case of a high concentration in which the electrical conductivity of the culture medium composition for promoting the growth of the sea breeze exceeds 5.5 dS m ^-1 , excessive waste of inorganic nutrients and osmotic stress may occur in the plant, which rather inhibits the growth of sea breeze plants. Occurs.

The gaetbangpung plant cultivated in this way is harvested by a conventional harvesting method and can be used as a raw material for functional foods or pharmaceuticals.

In one aspect of the present invention, the composition of the culture solution for promoting the growth of sea breeze (scientific name: Glehnia littoralis ) is similar to the ratio of inorganic ions preferred by sea breeze and adjusted to a specific electrical conductivity level, so that the conventional Hoagland nutrient solution In contrast, there is an effect of inducing relatively excellent growth promotion of sea breeze, which is directly supported by Examples, Comparative Examples, and Experimental Examples to be described later.

Hereinafter, the present invention will be described in detail through Examples and Experimental Examples.

However, the Examples and Experimental Examples to be described below are only to specifically illustrate the present invention in one aspect, but the present invention is not limited thereto.

<Example> Manufacture of nutrient solution (NS) exclusively for sea breeze

The culture solution for sea breeze is prepared by mixing the following A solution and B solution.

Based on 100 times the volume, solution A per volume 1L contains 66.08 g of calcium nitrate (Ca(NO ₃ ) ₂ 4H ₂ O), 42.925 g of potassium nitrate (KNO ₃ ), and 1.15 g of iron (Fe-EDTA). ,

Solution B is potassium nitrate (KNO ₃ ) 42.925g, monobasic ammonium phosphate (NH ₄ H ₂ PO ₄ ) 4.6g, potassium phosphate (KH ₂ PO ₄ ) 5.44g, magnesium sulfate (MgSO ₄ 7H ₂ O) 32.472g , copper sulfate (CuSO ₄ 5H ₂ O) 0.01768g, boron (H ₃ BO ₃ ) 0.20009g, manganese sulfate (MnSO ₄ 5H ₂ O) 0.24492g, zinc sulfate (ZnSO ₄ 7H ₂ O) 0.15391g, molybdenum It contains 0.01261 g of sodium acid (Na ₂ MoO ₄ 2H ₂ O).

Table 1 below shows the composition of A solution and B solution.

[Table 1]

Nutrient solution exclusively for windbreak (NS) refers to a culture solution for exclusive use of windbreak prepared by mixing Solution A and Solution B, and nutrient solution (NS) for exclusive use of windbreak by taking 1ml of each of the above A and B solutions and diluting them in 1L of raw water Per 1 L of volume, Ca(NO ₃ ) ₂ 4H ₂ O 66.08mg, KNO ₃ 85.85mg, Fe-EDTA 1.15mg, NH ₄ H ₂ PO ₄ 4.6mg, KH ₂ PO ₄ 5.44mg, MgSO ₄ 7H ₂ O 32.472 mg, CuSO ₄ 5H ₂ O 0.01768 mg, H ₃ BO ₃ 0.20009 mg, MnSO ₄ 5H ₂ O 0.24492 mg, ZnSO ₄ 7H ₂ O 0.15391 mg and Na ₂ MoO ₄ 2H ₂ O 0.01261 mg .

<Comparative Example> Hoagland nutrient solution (HL) preparation

A stock solution was prepared to prepare Hoagland's nutrient solution (HL) used as a conventional culture medium. Hoagland's culture medium was proposed in a paper by Hoagland and Arnon in 1938, and is the most commonly used culture medium for leafy vegetables and has high versatility. Hoagland's storage solution A contained 236.15 g of Ca(NO ₃ ) ₂ 4H ₂ O, 75.825 g of KNO ₃ , and 10.5275 g of Fe-EDTA per 1 L, and storage solution B contained 75.825 g of KNO ₃ and NH ₄ H ₂ per 1 L. PO ₄ 28.765 g, MgSO ₄ 7H ₂ O 123.24 g, CuSO ₄ 5H ₂ O 0.02 g, H ₃ BO ₃ 0.715 g, MnCl ₂ 4H ₂ O 0.4525 g, ZnSO ₄ 7H ₂ O 0.055 g and Na ₂ 0.005 g of MoO ₄ ·2H ₂ O. H1 was prepared by adding 2 ml of Hoagland stock solutions A and B per 1 L, and H2 was prepared by adding 4 ml of Hoagland stock solutions A and B per 1 L.

<Experimental Example 1> Comparison of cation and anion ratios in nutrient solution and sea breeze plants

The composition of the nutrient solution is determined according to the mineral absorption rate of the crop. Supplying nutrient solution at a rate similar to the nutrient absorption rate of crops can efficiently use fertilizer salts, reduce indiscriminate use of salts, and promote crop growth to increase yields. In this experiment, considering the average cation and anion nutrient absorption rate of the sea breeze, a nutrient solution dedicated to the sea breeze was developed, an appropriate concentration was selected, and the growth enhancement effect compared to the Hoagland nutrient solution generally used for leafy vegetable cultivation was confirmed.

Figure 1 and Tables 2 and 3 below show the nutrient solution (NS) prepared in Examples, the Hoagland nutrient solution (HL) prepared in Comparative Example, and the cations K, Ca, Mg and anion N in the seabreak plants, The result of comparing the ratio of P and S is shown.

[Table 2] Cation comparison results

[Table 3] Anion comparison results

As shown in Figure 1 and Tables 2 and 3, the nutrient solution (NS) dedicated to the sea breeze provided in one aspect of the present invention is very similar to the ratio of cations and anions in the sea breeze plant ( G. littoralis ) You can check.

On the other hand, in the case of the Hoagland nutrient solution (HL) prepared in Comparative Example, compared to the sea breeze plants, K in the cation was 18% lower, Mg was 6% higher, and Ca was 12% higher, so there is a big difference in composition there is In addition, Hoagland's nutrient solution (HL) in negative ions has a low ratio of N and a rather high ratio of S compared to seabreak plants, so there is a big difference in composition.

From this, the nutrient solution (NS) dedicated to sea breeze provided in one aspect of the present invention is very similar to the ratio of cations and anions in sea breeze plants ( G. littoralis ), and consists of a ratio of inorganic ions preferred by sea breeze It can be confirmed, and it can be seen that, compared to the conventional Hoagland nutrient solution, the nutrient solution (NS) dedicated to the sea breeze of the present invention can induce relatively excellent growth promotion of the sea breeze.

<Experimental Example 2> Evaluation of induction activity for promoting growth of sea breeze

Comparative evaluation of the nutrient solution (NS) prepared in Example and Hoagland nutrient solution (HL) prepared in Comparative Example for the growth promotion induction activity of the sea breeze, and evaluation of activity according to electrical conductivity EC (dS / m) For this purpose, the following experiments were performed.

More specifically, this experiment was carried out on 12/12 at a temperature of 20 ° C, relative humidity of 60%, carbon dioxide concentration of 550 ppm, light quality and luminous intensity of White LEDs + Red of 300 μmol m ^-2 s ^-1 PPFD (Photosynthetic Photon Flux Density). It was conducted for 18 weeks in a plant factory set to city (light/dark). Seabream seedlings were planted in a bile hydroponic system when there were two true leaves, and the culture medium treatment conditions were Hoagland nutrient solution (HL) EC 1.0 dS m ^-1 and 2.0 dS m ^-1 and the developed dedicated culture medium (NS) EC Each level (1.0, 2.0, 3.0, 4.0, 5.0 dS·m ⁻¹ ) was treated and cultivated, and the pH was maintained at 5.8±0.2 by correcting three times a week. Nutrient solutions were replaced every 2 weeks for all culture solutions. For a total of 18 weeks, the first leaves were harvested 8 weeks after planting, the second after 5 weeks, and the third after an additional 5 weeks.

The results are shown in Figures 2 to 5.

Figure 2 shows leaf live weight (Fig. 2. A), leaf dry weight (Fig. 2. B), leaf area (Fig. 2. C) and above-ground area (Fig. 2. D) is the result. Figure 3 is the average leaf live weight (Fig. 3. A), leaf dry weight (Fig. 3. B), leaf area (Fig. 3. C) and aboveground area (Fig. 3 .D) is the result. Figure 4 accumulates the results of the 1st, 2nd and 3rd harvests, and leaf live weight (Fig. 4. A), leaf dry weight (Fig. 4. B), leaf area (Fig. 4. C) and above-ground area (Fig. 4. D ) is the result showing. 2, 3, and 4, H1 and H2 are Hoagland EC 1.0 dS m ^-1 and 2.0 dS m ^-1 and N1, N2, N3, N4, and N5 are EC 1.0, 2.0, 3.0, 4.0, 5.0 dS·m ^-1 means.

), H2(

), N1(

) 그리고 N2(

)의 기존 설정한 pH 5.8에서 변화되는 pH 값을 절대값으로 표현한 결과이다. 검은 점선은 H1과 H2, 노란 점선은 N1과 N2의 18주간 주 3회 측정된 pH 변화 절대값의 평균을 표현한 것이다.Figure 5 shows H1 during the 18-week cultivation period (

), H2(

), N1(

) and N2(

) is the result of expressing the pH value that changes from the previously set pH 5.8 as an absolute value. The black dotted line represents the average of absolute values of pH change measured three times a week for 18 weeks in H1 and H2, and the yellow dotted line represents N1 and N2.

Referring to FIG. 2, as a result of the first leaf harvest, the dedicated nutrient solution EC 1.0 dS m ^-1 (N1) compared to Hoagland EC 1.0 dS m ^-1 (H1), leaf live weight, leaf area, and aboveground area were all 1.2 times, Leaf dry weight was 1.3 times higher, and in the case of secondary leaf harvest, N1 had 1.3 times, 1.2 times, 1.1 times, and 1.4 times higher leaf dry weight than H1 in leaf fresh weight, leaf area, aboveground area, and leaf dry weight, respectively. In the case of the 3rd leaf harvest, N1 also showed 1.6 times, 1.5 times, 1.4 times, and 1.5 times higher leaf fresh weight, leaf area, above-ground area, and leaf dry weight than H1, respectively. In the case of the culture medium for sea breeze by concentration, the growth of the 1st, 2nd, and 3rd harvests tended to decrease as the EC level increased. In particular, in the first harvest, compared to N5 (EC 5.0 dS·m ^-1 ), N1 was 2.1 times higher in aerial fresh weight, leaf area and above ground area, and 1.6 times higher in leaf dry weight. Compared to , leaf fresh weight, leaf area and leaf dry weight were all 2.2 times higher, and aerial part area was 2.1 times higher. As a result of the third harvest, N1 showed 1.9 and 1.7 times higher leaf fresh weight and leaf area, respectively, and 1.6 times higher aboveground area and leaf dry weight than N5.

In addition, referring to FIG. 3, as in FIG. 2, N1 showed 1.4 times higher leaf fresh weight and leaf dry weight, and 1.2 times higher leaf area and aerial part area than H1. In addition, N1 was 1.6 times higher in leaf area and leaf dry weight, and 1.7 times and 1.5 times higher in leaf fresh weight and aboveground area, respectively, than those of other dedicated nutrient solutions (N2, N3, N4, N5). Compared to this N5, it showed a statistically significant 2.1 times higher leaf live weight.

Furthermore, referring to FIG. 4, N1 showed the highest leaf fresh weight value at 33.8 g, which was 1.4 times higher than that of H1. In the case of leaf area, aerial part area, and leaf dry weight, N1 showed 1.3 times, 1.2 times, and 1.4 times higher results than H1, respectively.

Next, the pH of the nutrient solution directly affects the effectiveness of nutrients in relation to changes in the form or solubility of inorganic ions. When the pH rises, phosphoric acid and calcium combine to become insolubilized and the stability of iron decreases. Conversely, when the pH is lowered, excessive symptoms of specific ions appear due to an increase in the amount of absorption. Therefore, maintaining the proper pH can increase the shelf life of the culture medium for nutrient supply and is related to the effectiveness and ratio stability of inorganic ions in the culture medium. Figure 5 is the result of expressing the pH changes of H1, H2, N1 and N2 as absolute values during the 18-week cultivation period. The absolute value of pH change in N1 and N2 was 0.5, lower than the absolute value of 0.7 in H1 and H2. This means that the dedicated nutrient solution had less pH change in the culture medium compared to the Hoagland nutrient solution. Since the nutrient solution exclusively for seabreaking was developed based on the ratio of inorganic ions in the nutrient solution for seabreaking plants, it is judged that the ratio of inorganic ions in the nutrient solution will be more stable than Hoagland's nutrient solution. It is desirable to use a nutrient solution exclusively for sea breeze with a stable ratio of inorganic ions in the long term for sea breeze cultivation, where multiple leaf harvests are possible.

In conclusion, through three harvests during 18 weeks of cultivation, it was confirmed that the nutrient solution exclusively for the nutrient solution for the nutrient solution produced superior growth compared to the nutrient solution for Hoagland, and the nutrient solution exclusively for the nutrient solution for the nutrient solution developed was effective in maintaining the ratio of inorganic ions. , and in particular, it is suggested that EC 1.0 dS·m ^-1 (N1) is the optimal concentration of a nutrient solution dedicated to the production of sea breeze.

In summary, the nutrient solution (NS) for sea breeze provided in one aspect of the present invention,

Ca(NO ₃ ) ₂ 4H ₂ O 66.08mg, KNO ₃ 85.85mg, Fe-EDTA 1.15mg, NH ₄ H ₂ PO ₄ 4.6mg, KH ₂ PO ₄ 5.44mg, MgSO ₄ 7H ₂ O 32.472 per 1L volume mg, CuSO ₄ 5H ₂ O 0.01768 mg, H ₃ BO ₃ 0.20009 mg, MnSO ₄ 5H ₂ O 0.24492 mg, ZnSO ₄ 7H ₂ O 0.15391 mg and Na ₂ MoO ₄ 2H ₂ O 0.01261 mg; At the same time, the electrical conductivity EC (dS / m) of 1.0 dS · m ^-1 condition (level) is the most optimal sea breeze-only culture solution, which was derived after a high degree of inventive effort.

Claims (9)

Culture medium A containing 65 to 67 parts by weight of calcium nitrate (Ca(NO ₃ ) ₂ .4H ₂ O) and 0.9 to 1.3 parts by weight of iron (Fe-EDTA) based on 42.925 parts by weight of potassium nitrate (KNO ₃ ); and
Based on 42.925 parts by weight of potassium nitrate (KNO ₃ ), 4.0 to 5.0 parts by weight of ammonium phosphate (NH ₄ H ₂ PO ₄ ), 5.0 to 6.0 parts by weight of potassium phosphate (KH ₂ PO ₄ ), magnesium sulfate (MgSO ₄ ) 7H ₂ O) 32 to 33 parts by weight, copper sulfate (CuSO ₄ 5H ₂ O) 0.01 to 0.02 parts by weight, boron (H ₃ BO ₃ ) 0.15 to 0.25 parts by weight, manganese sulfate (MnSO ₄ 5H ₂ O) 0.2 to 0.25 parts by weight 0.3 parts by weight, zinc sulfate (ZnSO ₄ 7H ₂ O) 0.1 to 0.2 parts by weight, sodium molybdate (Na ₂ MoO ₄ 2H ₂ O) 0.01 to 0.02 parts by weight of culture medium B containing; , Sea breeze (scientific name: Glehnia littoralis ) Culture composition for promoting growth. According to claim 1,
The culture composition,
Based on potassium nitrate (KNO ₃ ) 42.925 parts by weight, calcium nitrate (Ca(NO ₃ ) ₂ 4H ₂ O) 66.08
Culture medium A containing 1.15 parts by weight of iron (Fe-EDTA); and
Based on potassium nitrate (KNO ₃ ) 42.925 parts by weight, ammonium phosphate monobasic (NH ₄ H ₂ PO ₄ ) 4.6 parts by weight, potassium phosphate (KH ₂ PO ₄ ) 5.44 parts by weight, magnesium sulfate (MgSO ₄ 7H ₂ O) 32.472 parts by weight, copper sulfate (CuSO ₄ 5H ₂ O) 0.01768 parts by weight, boron (H ₃ BO ₃ ) 0.20009 parts by weight, manganese sulfate (MnSO ₄ 5H ₂ O) 0.24492 parts by weight, zinc sulfate (ZnSO ₄ 7H ₂ O) 0.15391 parts by weight, culture medium B containing 0.01261 parts by weight of sodium molybdate (Na ₂ MoO ₄ 2H ₂ O). Ca(NO ₃ ) ₂ 4H ₂ O 66.08g, KNO ₃ 42.925g, Fe-EDTA 1.15g per 1L volume
Culture A; and
42.925 g of KNO ₃ , 4.6 g of NH ₄ H ₂ PO ₄ , 5.44 g of KH ₂ PO ₄ , 32.472 g of MgSO ₄ 7H ₂ O,
_culture medium B containing 0.01768 g of CuSO ₄ 5H ₂ O, 0.20009 g of H ₃ BO ₃ , 0.24492 g of MnSO 4 5H ₂ O, 0.15391 g of ZnSO ₄ 7H ₂ O and 0.01261 g of Na ₂ MoO ₄ 2H ₂ O; Characterized in that it comprises, the sea breeze (scientific name: Glehnia littoralis) growth promoting culture medium composition.
Ca(NO ₃ ) ₂ 4H ₂ O 66.08mg, KNO ₃ 85.85mg, Fe-EDTA 1.15mg, NH ₄ H ₂ PO ₄ 4.6mg, KH ₂ PO ₄ 5.44mg, MgSO ₄ 7H ₂ O 32.472 per 1L volume mg, CuSO ₄ 5H ₂ O 0.01768 mg, H ₃ BO ₃ 0.20009 mg, MnSO ₄ 5H ₂ O 0.24492 mg, ZnSO ₄ 7H ₂ O 0.15391 mg, and Na ₂ MoO ₄ 2H ₂ O 0.01261 mg Characterized in, sea breeze (scientific name: Glehnia littoralis ) Culture composition for promoting growth. According to claim 4,
Electrical conductivity (EC, Electrical Conductivity) of the culture medium composition is 0.8 to 1.2 dS · m ^-1 Characterized in that, the windbreak (scientific name: Glehnia littoralis ) Culture medium composition for promoting growth. According to claim 5,
Electrical conductivity (EC, Electrical Conductivity) of the culture solution composition is 0.9 to 1.1 dS · m ^-1 , characterized in that, the windbreak (scientific name: Glehnia littoralis ) growth promotion culture solution composition.
Using the culture composition of claim 4, a method of cultivating sea breeze (scientific name: Glehnia littoralis ) plants.
According to claim 7,
The cultivation method of the sea breeze plant,
A planting step of planting seedlings of sea breeze plants in hydroponic cultivation facilities;
Supplying carbon dioxide in the range of 400 to 1000 ppm to the sea breeze plant under a light intensity in the range of 100 to 500 μmol m ^-2 s ^-1 , maintaining a temperature of 15 to 25 ° C, and applying the culture solution composition of claim 4 A growth step of growing by doing; and
A method of cultivating a sea breeze (scientific name: Glehnia littoralis ) plant, comprising: harvesting a sea breeze plant that has undergone the growth step.
According to claim 8,
In the growth stage, the light intensity is in the range of 250 to 350 μmol m ^-2 s ^-1 , carbon dioxide is in the range of 500 to 600 ppm, and the temperature is in the range of 19 to 21 ° C. cultivation method.

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