KR20230074505A - 원하는 특이성을 갖는 이펙터 세포의 제조 방법 - Google Patents
원하는 특이성을 갖는 이펙터 세포의 제조 방법 Download PDFInfo
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| WO2014165707A2 (en) | 2013-04-03 | 2014-10-09 | Memorial Sloan-Kettering Cancer Center | Effective generation of tumor-targeted t-cells derived from pluripotent stem cells |
| WO2016010155A1 (ja) | 2014-07-18 | 2016-01-21 | 宏 河本 | 抗原特異的ｔ細胞受容体遺伝子を有する多能性幹細胞の製造方法 |
| WO2016010153A1 (ja) | 2014-07-18 | 2016-01-21 | 宏 河本 | 免疫細胞療法用ｔ細胞の誘導方法 |
| WO2016010154A1 (ja) | 2014-07-18 | 2016-01-21 | 宏 河本 | 抗原特異的ｔ細胞受容体遺伝子を有する多能性幹細胞の製造方法 |
| WO2017179720A1 (ja) | 2016-04-15 | 2017-10-19 | 国立大学法人京都大学 | Ｃｄ８陽性ｔ細胞を誘導する方法 |
| WO2020022512A1 (ja) | 2018-07-26 | 2020-01-30 | 国立大学法人京都大学 | 外来抗原レセプター遺伝子導入細胞の製造方法 |
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| CN105316362B (zh) | 2015-08-19 | 2020-03-17 | 暨南大学 | 一种Dual-RMCE介导的TCR基因置换系统及其方法 |
| US20200109364A1 (en) | 2018-05-31 | 2020-04-09 | Washington University | Methods for genome-editing and activation of cells |
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Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2014165707A2 (en) | 2013-04-03 | 2014-10-09 | Memorial Sloan-Kettering Cancer Center | Effective generation of tumor-targeted t-cells derived from pluripotent stem cells |
| WO2016010155A1 (ja) | 2014-07-18 | 2016-01-21 | 宏 河本 | 抗原特異的ｔ細胞受容体遺伝子を有する多能性幹細胞の製造方法 |
| WO2016010153A1 (ja) | 2014-07-18 | 2016-01-21 | 宏 河本 | 免疫細胞療法用ｔ細胞の誘導方法 |
| WO2016010154A1 (ja) | 2014-07-18 | 2016-01-21 | 宏 河本 | 抗原特異的ｔ細胞受容体遺伝子を有する多能性幹細胞の製造方法 |
| WO2017179720A1 (ja) | 2016-04-15 | 2017-10-19 | 国立大学法人京都大学 | Ｃｄ８陽性ｔ細胞を誘導する方法 |
| WO2020022512A1 (ja) | 2018-07-26 | 2020-01-30 | 国立大学法人京都大学 | 外来抗原レセプター遺伝子導入細胞の製造方法 |
Non-Patent Citations (2)
| Title |
|---|
| Gong Ying et al., Journal of Cell Biology (2015) 1481-1489 |
| Themeli et al., Nat Biotechnol. (2013) 928-33 |
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| EP4219707A1 (en) | 2023-08-02 |
| CN116406423A (zh) | 2023-07-07 |
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| US20230357715A1 (en) | 2023-11-09 |
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