KR20230065295A - 유전자 라이브러리 및 유전자 분석의 고효율성 구축을 위한 어댑터 및 방법 - Google Patents

유전자 라이브러리 및 유전자 분석의 고효율성 구축을 위한 어댑터 및 방법 Download PDF

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KR20230065295A
KR20230065295A KR1020237011639A KR20237011639A KR20230065295A KR 20230065295 A KR20230065295 A KR 20230065295A KR 1020237011639 A KR1020237011639 A KR 1020237011639A KR 20237011639 A KR20237011639 A KR 20237011639A KR 20230065295 A KR20230065295 A KR 20230065295A
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dna
adapter
multifunctional
nucleotides
region
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Korean (ko)
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지안난 구오
제니퍼 헤르난데즈
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레졸루션 바이오사이언스, 인크.
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
KR1020237011639A 2020-09-08 2021-09-08 유전자 라이브러리 및 유전자 분석의 고효율성 구축을 위한 어댑터 및 방법 Pending KR20230065295A (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202063075543P 2020-09-08 2020-09-08
US63/075,543 2020-09-08
PCT/US2021/049448 WO2022055984A1 (en) 2020-09-08 2021-09-08 Adaptors and methods for high efficiency construction of genetic libraries and genetic analysis

Publications (1)

Publication Number Publication Date
KR20230065295A true KR20230065295A (ko) 2023-05-11

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KR1020237011639A Pending KR20230065295A (ko) 2020-09-08 2021-09-08 유전자 라이브러리 및 유전자 분석의 고효율성 구축을 위한 어댑터 및 방법

Country Status (13)

Country Link
US (1) US20220073906A1 (https=)
EP (1) EP4211246B1 (https=)
JP (1) JP2023540782A (https=)
KR (1) KR20230065295A (https=)
CN (1) CN116490613A (https=)
AU (1) AU2021340632A1 (https=)
CA (1) CA3189103A1 (https=)
DK (1) DK4211246T3 (https=)
ES (1) ES3045482T3 (https=)
HU (1) HUE072019T2 (https=)
PL (1) PL4211246T3 (https=)
PT (1) PT4211246T (https=)
WO (1) WO2022055984A1 (https=)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111254500B (zh) 2012-12-10 2024-01-23 分析生物科学有限公司 靶向基因组分析的方法
US20160053301A1 (en) 2014-08-22 2016-02-25 Clearfork Bioscience, Inc. Methods for quantitative genetic analysis of cell free dna
US11319594B2 (en) 2016-08-25 2022-05-03 Resolution Bioscience, Inc. Methods for the detection of genomic copy changes in DNA samples
CN115029413A (zh) * 2022-05-17 2022-09-09 翌圣生物科技(上海)股份有限公司 一种dna和/或rna快速建库的方法和试剂盒
WO2024049276A1 (ko) * 2022-09-01 2024-03-07 주식회사 키오믹스 다중 표적 dna의 선택적 증폭용 조성물 및 이를 이용한 증폭 방법

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115029342B (zh) * 2014-08-22 2025-04-08 分析生物科学有限公司 用于无细胞dna的定量遗传分析的方法
JP6913089B2 (ja) * 2015-11-11 2021-08-04 レゾリューション バイオサイエンス， インコーポレイテッド Ｄｎａライブラリーの高効率構築
US11708574B2 (en) * 2016-06-10 2023-07-25 Myriad Women's Health, Inc. Nucleic acid sequencing adapters and uses thereof
GB201615486D0 (en) 2016-09-13 2016-10-26 Inivata Ltd Methods for labelling nucleic acids
US11268137B2 (en) * 2016-12-09 2022-03-08 Boreal Genomics, Inc. Linked ligation
WO2018119399A1 (en) * 2016-12-23 2018-06-28 Grail, Inc. Methods for high efficiency library preparation using double-stranded adapters
AU2018212756B2 (en) 2017-01-27 2021-02-25 Integrated Dna Technologies, Inc. Construction of next generation sequencing (NGS) libraries using competitive strand displacement
EP3885445B1 (en) * 2017-04-14 2023-08-23 Guardant Health, Inc. Methods of attaching adapters to sample nucleic acids
AU2019207900B2 (en) 2018-01-12 2025-07-10 Claret Bioscience, Llc Methods and compositions for analyzing nucleic acid
JP2022513124A (ja) * 2018-11-21 2022-02-07 アヴィダ バイオメッド， インコーポレイテッド 標的化された核酸ライブラリー形成のための方法
EP3899031B1 (en) 2018-12-19 2025-07-30 New England Biolabs, Inc. Methods for nucleic acid target enrichment

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Publication number Publication date
EP4211246B1 (en) 2025-07-23
DK4211246T3 (da) 2025-08-18
US20220073906A1 (en) 2022-03-10
WO2022055984A1 (en) 2022-03-17
EP4211246A1 (en) 2023-07-19
HUE072019T2 (hu) 2025-10-28
PL4211246T3 (pl) 2025-10-20
EP4211246A4 (en) 2024-07-24
PT4211246T (pt) 2025-07-30
CN116490613A (zh) 2023-07-25
ES3045482T3 (en) 2025-11-28
JP2023540782A (ja) 2023-09-26
AU2021340632A1 (en) 2023-03-16
CA3189103A1 (en) 2022-03-17

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