ES3045482T3 - Adaptors and methods for high efficiency construction of genetic libraries and genetic analysis - Google Patents

Adaptors and methods for high efficiency construction of genetic libraries and genetic analysis

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Publication number
ES3045482T3
ES3045482T3 ES21867505T ES21867505T ES3045482T3 ES 3045482 T3 ES3045482 T3 ES 3045482T3 ES 21867505 T ES21867505 T ES 21867505T ES 21867505 T ES21867505 T ES 21867505T ES 3045482 T3 ES3045482 T3 ES 3045482T3
Authority
ES
Spain
Prior art keywords
dna
adapter
multifunctional
region
approximately
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
ES21867505T
Other languages
English (en)
Spanish (es)
Inventor
Jiannan Guo
Jennifer Hernandez
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Resolution Bioscience Inc
Original Assignee
Resolution Bioscience Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Resolution Bioscience Inc filed Critical Resolution Bioscience Inc
Application granted granted Critical
Publication of ES3045482T3 publication Critical patent/ES3045482T3/es
Active legal-status Critical Current
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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1065Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6853Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855Ligating adaptors

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
ES21867505T 2020-09-08 2021-09-08 Adaptors and methods for high efficiency construction of genetic libraries and genetic analysis Active ES3045482T3 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202063075543P 2020-09-08 2020-09-08
PCT/US2021/049448 WO2022055984A1 (en) 2020-09-08 2021-09-08 Adaptors and methods for high efficiency construction of genetic libraries and genetic analysis

Publications (1)

Publication Number Publication Date
ES3045482T3 true ES3045482T3 (en) 2025-11-28

Family

ID=80469537

Family Applications (1)

Application Number Title Priority Date Filing Date
ES21867505T Active ES3045482T3 (en) 2020-09-08 2021-09-08 Adaptors and methods for high efficiency construction of genetic libraries and genetic analysis

Country Status (13)

Country Link
US (1) US20220073906A1 (https=)
EP (1) EP4211246B1 (https=)
JP (1) JP2023540782A (https=)
KR (1) KR20230065295A (https=)
CN (1) CN116490613A (https=)
AU (1) AU2021340632A1 (https=)
CA (1) CA3189103A1 (https=)
DK (1) DK4211246T3 (https=)
ES (1) ES3045482T3 (https=)
HU (1) HUE072019T2 (https=)
PL (1) PL4211246T3 (https=)
PT (1) PT4211246T (https=)
WO (1) WO2022055984A1 (https=)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111254500B (zh) 2012-12-10 2024-01-23 分析生物科学有限公司 靶向基因组分析的方法
US20160053301A1 (en) 2014-08-22 2016-02-25 Clearfork Bioscience, Inc. Methods for quantitative genetic analysis of cell free dna
US11319594B2 (en) 2016-08-25 2022-05-03 Resolution Bioscience, Inc. Methods for the detection of genomic copy changes in DNA samples
CN115029413A (zh) * 2022-05-17 2022-09-09 翌圣生物科技(上海)股份有限公司 一种dna和/或rna快速建库的方法和试剂盒
WO2024049276A1 (ko) * 2022-09-01 2024-03-07 주식회사 키오믹스 다중 표적 dna의 선택적 증폭용 조성물 및 이를 이용한 증폭 방법

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115029342B (zh) * 2014-08-22 2025-04-08 分析生物科学有限公司 用于无细胞dna的定量遗传分析的方法
JP6913089B2 (ja) * 2015-11-11 2021-08-04 レゾリューション バイオサイエンス, インコーポレイテッド Dnaライブラリーの高効率構築
US11708574B2 (en) * 2016-06-10 2023-07-25 Myriad Women's Health, Inc. Nucleic acid sequencing adapters and uses thereof
GB201615486D0 (en) 2016-09-13 2016-10-26 Inivata Ltd Methods for labelling nucleic acids
US11268137B2 (en) * 2016-12-09 2022-03-08 Boreal Genomics, Inc. Linked ligation
WO2018119399A1 (en) * 2016-12-23 2018-06-28 Grail, Inc. Methods for high efficiency library preparation using double-stranded adapters
AU2018212756B2 (en) 2017-01-27 2021-02-25 Integrated Dna Technologies, Inc. Construction of next generation sequencing (NGS) libraries using competitive strand displacement
EP3885445B1 (en) * 2017-04-14 2023-08-23 Guardant Health, Inc. Methods of attaching adapters to sample nucleic acids
AU2019207900B2 (en) 2018-01-12 2025-07-10 Claret Bioscience, Llc Methods and compositions for analyzing nucleic acid
JP2022513124A (ja) * 2018-11-21 2022-02-07 アヴィダ バイオメッド, インコーポレイテッド 標的化された核酸ライブラリー形成のための方法
EP3899031B1 (en) 2018-12-19 2025-07-30 New England Biolabs, Inc. Methods for nucleic acid target enrichment

Also Published As

Publication number Publication date
EP4211246B1 (en) 2025-07-23
DK4211246T3 (da) 2025-08-18
US20220073906A1 (en) 2022-03-10
KR20230065295A (ko) 2023-05-11
WO2022055984A1 (en) 2022-03-17
EP4211246A1 (en) 2023-07-19
HUE072019T2 (hu) 2025-10-28
PL4211246T3 (pl) 2025-10-20
EP4211246A4 (en) 2024-07-24
PT4211246T (pt) 2025-07-30
CN116490613A (zh) 2023-07-25
JP2023540782A (ja) 2023-09-26
AU2021340632A1 (en) 2023-03-16
CA3189103A1 (en) 2022-03-17

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