KR20230059207A - Composition for inner beauty cosmetics from broccoli leaves fraction extract and manufacturing method thereof - Google Patents
Composition for inner beauty cosmetics from broccoli leaves fraction extract and manufacturing method thereof Download PDFInfo
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- KR20230059207A KR20230059207A KR1020210143139A KR20210143139A KR20230059207A KR 20230059207 A KR20230059207 A KR 20230059207A KR 1020210143139 A KR1020210143139 A KR 1020210143139A KR 20210143139 A KR20210143139 A KR 20210143139A KR 20230059207 A KR20230059207 A KR 20230059207A
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- South Korea
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- broccoli
- uniformly mixed
- main tank
- inner beauty
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- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/52—Stabilizers
- A61K2800/522—Antioxidants; Radical scavengers
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Abstract
Description
본 발명은 먹는 화장품인 이너뷰티 화장품에 관한 것으로서, 특히 브로콜리의 비가식 부위인 잎을 활용하여 효능과 안정성을 갖는 기능성 화장품을 제공하기 위한 브로콜리 잎 분획물을 이용한 이너뷰티 조성물 및 그 제조방법에 관한 것이다.The present invention relates to inner beauty cosmetics, which are edible cosmetics, and more particularly, to an inner beauty composition using a broccoli leaf fraction and a manufacturing method thereof to provide functional cosmetics having efficacy and stability by utilizing leaves, which are non-edible parts of broccoli. .
최근 화장품 소비자의 제품을 선택하는 주요 요인은 과거의 '브랜드 인지도'에서 '목적성·기능성'으로 옮겨지고 있는 경향을 보이고 있으며, 환경호르몬이나 유전자변형에 따른 화장품 성분에 대한 문제점이 심각하게 대두되면서 '천연성'을 선호하는 소비자 심리가 반영되어 천연물을 중심으로 한 기능성 화장품의 수요가 증가하고 있는 실정이다.Recently, the main factor in choosing cosmetics consumers' products tends to be shifted from 'brand awareness' in the past to 'purpose/functionality'. Consumer psychology that prefers 'naturalness' is reflected, and the demand for functional cosmetics centered on natural products is increasing.
생명공학기술의 발달로 피부세포의 분화, 노화현상의 규명, 인공세포의 개발 등 피부과학에 대한 연구가 활발해짐에 따라 이러한 소비트렌드에 발맞추어 다양한 기능성 천연 화장품이 개발되었으며, 미백, 보습, 자외선 차단 및 흡수, 유해산소 제거, 콜라겐 합성, 피부주름방지 등의 다양한 효능을 가진 천연 소재에 대한 관심도 증대되고 있다.As research on skin science, such as differentiation of skin cells, identification of aging phenomena, and development of artificial cells, has become active with the development of biotechnology, various functional natural cosmetics have been developed in line with these consumption trends, and whitening, moisturizing, and ultraviolet rays have been developed. Interest in natural materials with various effects such as blocking and absorbing, removing free radicals, synthesizing collagen, and preventing skin wrinkles is also increasing.
최근 천연 소재의 기능성 화장품에 있어서 소비자의 주요 니즈는, 복합 기능성 소재 요구도 증가와, 유기농 화장품 수요 증가 및 화장품 용도 외의 고기능성 부여(이너뷰티)의 세 가지로 분류할 수 있다.Recently, the main needs of consumers for functional cosmetics made of natural materials can be classified into three categories: increasing demand for complex functional materials, increasing demand for organic cosmetics, and imparting high functionality other than cosmetic use (inner beauty).
첫 번째 소비자 니즈인 복합 기능성 소재 요구도 증가는, 최근 여러 기능을 한 번에 해결할 수 있는 다양한 콘셉트의 '올인원(All-in-One)' 제품에 대한 소비자의 관심이 증대되고 있는 것을 말한다. The first consumer need, the increase in demand for composite functional materials, refers to the recent increase in consumer interest in 'All-in-One' products with various concepts that can solve multiple functions at once.
두 번째 소비자 니즈인 유기농 화장품 수요 증가는, 최근 들어 천연소재, 인체에 무해한 소재 등에 대한 소비자의 큰 관심과, 이에 따라 유기농 재배를 통해 추출된 천연물을 활용한 유기농 화장품의 수요도 증가되고 있는 것을 말한다.The second consumer need, the increase in demand for organic cosmetics, refers to the recent high consumer interest in natural materials and materials harmless to the human body, and accordingly, the demand for organic cosmetics using natural products extracted through organic cultivation is also increasing. .
소비자 니즈의 마지막 세 번째인 화장품 용도 외의 고기능성 부여(이너뷰티)는, 코스메슈티컬(Cosmeceutical: cosmetics와 pharmaceutical의 합성어)이라는 단어가 등장할 만큼 단순한 화장품을 뛰어넘는 효능을 소비자들이 바라고 있으며, 이에 대한 소재 개발도 활발히 진행되고 있음을 나타낸다.As for the third and final consumer need, imparting high functionality (inner beauty) other than cosmetic use, consumers want efficacy that goes beyond simple cosmetics to the extent that the word cosmeceutical (cosmeceutical: a compound word of cosmetics and pharmaceutical) appears. This indicates that material development is also actively progressing.
이에 따라 신체 건강과 피부 건강을 동시에 충족시킬 수 있는, 즉 섭취가 가능한 화장품(이너뷰티, Inner Beauty) 시장이 급부상 하고 있다. 기존의 영양제나 비타민과 달리 이너뷰티 화장품은 유효한 성분들로 집중 구성되어 피부에 직접적인 효과를 부여할 뿐만 아니라 휴대성이 간편한 스틱이나 젤리 타입 등으로 제조되어 언제 어디서든지 간편히 섭취가 가능하기 때문에 소비자들에게 큰 호응을 받고 있다.Accordingly, the market for ingestible cosmetics (Inner Beauty), which can satisfy both physical health and skin health, is rapidly emerging. Unlike existing nutritional supplements or vitamins, inner beauty cosmetics are composed of concentrated active ingredients that not only give direct effects to the skin, but are also manufactured in a portable stick or jelly type, so they can be easily consumed anytime, anywhere, so consumers is receiving great acclaim.
건강한 아름다움을 중시하는 트렌드가 확산되면서 건강기능식품을 통해 뷰티 고민을 해결하고자 하는 니즈가 증가하고 있으며, 이와 같은 변화에 따라 피부미용, 다이어트 등 2030 여성들의 니즈에 맞는 다양한 제품들이 잇따라 출시되면서 시장이 한층 다양화되고 있다. As the trend that emphasizes healthy beauty is spreading, the need to solve beauty concerns through health functional foods is increasing. In accordance with this change, various products such as skin care and diet that meet the needs of women in their 20s and 30s are being released one after another, and the market is expanding. becoming more diversified.
이너뷰티(미용음료 포함) 시장은 최근 미용과 건강에 대한 관심이 증가함에 따라 미국과 유럽 등 서구에서도 복용형태로 자외선 차단제품, 주름개선, 피부보습, 피부영양 개선 등 미용 목적의 제품개발이 연구되어 코스메슈티컬 제품들과 로레알, P&G 등 거대 화장품 기어의 참여로 시장에서 제품의 신뢰도 증가와 함께 시장은 크게 성장하고 있다.In the inner beauty (including beauty beverage) market, as interest in beauty and health has recently increased, Western countries such as the U.S. and Europe are also researching the development of products for beauty purposes such as sunscreen products in dosage form, wrinkle improvement, skin moisturizing, and skin nutrition improvement. With the participation of cosmeceutical products and giant cosmetics gear such as L'Oreal and P&G, the market is growing significantly with the increase in reliability of products in the market.
이너뷰티 화장품은 일반 식품과 달리 인체 안전성과 기능성을 중시하고 있으므로 건강기능식품과 동일시할 만큼 소비자의 선택에 있어 주요 원료에 대한 중요성이 확대되고 있다. 따라서 이너뷰티 화장품은 소재의 안전성과 기능성이 중요한 선택의 척도가 되고 있으며, 이너뷰티 제품이 건강기능식품의 규제를 직접 받는 것은 아니지만 소비자의 눈높이를 감안할 때 정부의 건강기능식품 품질관리 규정을 고려하여 제품을 개발할 필요가 있다. Inner beauty cosmetics, unlike general foods, place importance on human safety and functionality, so the importance of major raw materials in consumer choice is expanding to the extent that they are equated with health functional foods. Therefore, for inner beauty cosmetics, the safety and functionality of the material is an important selection criterion. Although inner beauty products are not directly subject to health functional food regulations, considering the level of consumers' eyes, You need to develop a product.
특히, 현재 이너뷰티 화장품의 소재로는 고가의 해외 수입 원료가 대부분을 차지하고 있기 때문에 제품 제조에 고비용이 발생하고 이는 소비자에게 높은 가격으로 제공되고 있으므로, 이를 해결하기 위하여 다양한 천연물 소재의 개발이 요구되고 있으며, 고부가가치 식품 산업의 발전과 이너뷰티 제품을 통한 또 다른 케이뷰티(K-Beauty), 케이푸드(K-Food)의 열풍을 이어받아 세계 시장에서 국격을 높일 수 있는 기술개발이 시급하다. In particular, since most of the expensive imported raw materials are currently used in inner beauty cosmetics, high costs are incurred in manufacturing products, which are provided to consumers at high prices. To solve this problem, development of various natural materials is required. In addition, it is urgent to develop technology that can enhance national prestige in the global market by inheriting the craze of another K-Beauty and K-Food through the development of the high value-added food industry and inner beauty products.
이에 본 발명자는 상술한 제반 사항을 종합적으로 고려하여 이너뷰티 화장품의 소재로 활용할 수 있는 천연물 소재를 개발하던 중, 건강식품으로 잘 알려져 있는 브로콜리가 가식부위의 주요 생리활성물질과 그 효능이 잘 알려진 반면에 비가식 부위인 잎에 대해서는 장류 발효 개발에 관한 보고만 있을 뿐 기능적 생리활성을 다른 연구가 미비함에 따라, 브로콜리의 비가식 부위인 잎을 생리활성 성분과 함량 분석 및 피부 생리활성에 대한 효능 검증을 통해 기능성 소재로 개발하고자 각고의 노력을 기울여 부단히 연구하던 중 그 결과로써 본 발명을 창안하게 되었다.Accordingly, while the present inventors were developing natural materials that can be used as materials for inner beauty cosmetics by comprehensively considering the above-mentioned matters, broccoli, which is well known as a health food, is a major physiologically active substance in edible parts and its efficacy is well known. On the other hand, for leaves, which are non-edible parts, there are only reports on the development of fermented soybean paste fermentation, but as other studies on functional physiological activity are lacking, the leaves, which are non-edible parts of broccoli, are analyzed for their physiologically active components and contents, and their efficacy on skin physiological activity To develop it as a functional material through verification, the present invention was created as a result of continuous research with painstaking efforts.
따라서 본 발명이 해결하고자 하는 기술적 과제 및 목적은 브로콜리의 비가식 부위인 잎을 용매별로 물과 에탄올 추출물을 분획하여 검증한 생리활성 성분과 함량 분석 및 피부 생리활성 효능에 적합한 제형으로 구현할 수 있는 브로콜리 잎 분획물을 이용한 이너뷰티 조성물 및 그 제조방법을 제공하는 데 있는 것이다.Therefore, the technical problem and object to be solved by the present invention is to separate the leaves, which are non-edible parts of broccoli, into water and ethanol extracts for each solvent, analyze the verified bioactive components and contents, and broccoli that can be implemented as a formulation suitable for skin physiological activity efficacy It is to provide an inner beauty composition using a leaf fraction and a manufacturing method thereof.
또한, 본 발명이 해결하고자 하는 또 다른 기술적 과제 및 목적은 분획물의 생리활성 성분의 안정성과 효능을 지속시킬 수 있으며, 피부 친화력과 침투력을 증진시킬 수 있는 제형을 적용한 브로콜리 잎 분획물을 이용한 이너뷰티 조성물 및 그 제조방법을 제공하는 데 있는 것이다.In addition, another technical problem and object to be solved by the present invention is an inner beauty composition using a broccoli leaf fraction to which a formulation capable of maintaining the stability and efficacy of the bioactive components of the fraction and enhancing skin affinity and penetration is applied And it is to provide a manufacturing method thereof.
여기서 본 발명이 해결하고자 하는 기술적 과제 및 목적은 이상에서 언급한 기술적 과제 및 목적으로 국한하지 않으며, 언급하지 않은 또 다른 기술적 과제 및 목적들은 아래의 기재로부터 당업자가 명확하게 이해할 수 있을 것이다.Here, the technical problems and objects to be solved by the present invention are not limited to the technical problems and objects mentioned above, and other technical problems and objects not mentioned will be clearly understood by those skilled in the art from the following description.
상술한 목적을 달성하기 위한 본 발명에 따른 브로콜리 잎 분획물을 이용한 이너뷰티 조성물은, 전체 100 중량%에 대하여, 정제수 40.0-50.0 중량%, 폴리리신 5.0-10.0 중량%, 글리세린 1.0-5.0 중량%, 펜틸렌글라이콜 1.0-5.0 중량%, 하이드록시프로필메틸셀룰로오스 1.0-5.0 중량%, 나이아신아마이드 1.0-5.0 중량%, 아데노신 0.01-0.05 중량%, 다이소듐이디티에이 0.01-0.05 중량%, 프로필렌글라이콜다이카프릴레이트/다이카프레이트 10.0-15.0 중량%, 사이클로펜타실록세인 1.0-5.0 중량%, 사이클로헥사실록세인 1.0-5.0 중량%, 세테아릴올리베이트 1.0-5.0 중량%, 솔비탄올리베이트 1.0-5.0 중량%, 카프릴릭/카프릭트라이글리세라이드 1.0-5.0 중량%, 티타늄디옥사이드 0.5-1.0 중량%, 알루미늄하이드록사이드 0.1-0.5 중량%, 트라이에톡시카프릴릴실레인 0.1-0.5 중량%, 다이메티콘 0.1-0.5 중량%, 비닐다이메티콘크로스폴리머 0.5-1.0 중량%, 하이드록시에틸아크릴레이트/소듐아크릴로일다이메틸타우레이트코폴리머 0.5-1.0 중량%, 카보머 0.1-0.5 중량%, 토코페릴아세테이트 0.1-0.5 중량%, 클로록실레놀 0.1-0.5 중량%, 1,2-헥산다이올 0.1-0.5 중량%, 클로르페네신 0.1-0.5 중량%, 브로콜리잎/뿌리추출물 0.05-0.1 중량%, 병풀추출물 0.05-0.1 중량%, 하이드롤라이즈드글라이코사미노글리칸 0.1-0.5 중량%, 소듐하이알루로네이트 0.1-0.5 중량%, 소듐하이알루로네이트크로스폴리머 0.1-0.5 중량%, 하이드롤라이즈드하이알루로닉애씨드 0.1-0.5 중량%, 하이드록시프로필트라이모늄하이알루로네이트 0.1-0.5 중량%, 벤질글라이콜>세라마이드엔피 0.1-0.5 중량%, 소듐아세틸레이티드하이알루로네이트 0.1-0.5 중량%, 다이포타슘글리시리제이트 0.05-0.1 중량%, 에틸헥실글리세린>폴리옥시에칠렌글리세릴모노스테아레이트 0.1-0.5 중량%, 시트릭애씨드 0.1-0.5 중량% 및 향료 0.05-0.1 중량%을 함유하는 것을 특징으로 한다. The inner beauty composition using the broccoli leaf fraction according to the present invention for achieving the above object is 40.0-50.0% by weight of purified water, 5.0-10.0% by weight of polylysine, glycerin, based on 100% by weight of the total 1.0-5.0 wt%, pentylene glycol 1.0-5.0 wt%, hydroxypropylmethylcellulose 1.0-5.0 wt%, niacinamide 1.0-5.0 wt%, adenosine 0.01-0.05 wt%, disodium EDTA 0.01-0.05 wt%, propylene glycol dicaprylate/dicaprate 10.0-15.0 wt%, cyclopentasiloxane 1.0-5.0 wt%, cyclohexasiloxane 1.0 -5.0 wt%, cetearyl olivate 1.0-5.0 wt%, sorbitan olivate 1.0-5.0 wt%, caprylic/capric triglyceride 1.0-5.0 wt%, titanium dioxide 0.5-1.0 wt%, aluminum hydride 0.1-0.5% by weight of oxide, 0.1-0.5% by weight of triethoxycaprylylsilane, 0.1-0.5% by weight of dimethicone, 0.5-1.0% by weight of vinyldimethicone crosspolymer, hydroxyethyl acrylate/sodium Acryloyldimethyltaurate copolymer 0.5-1.0% by weight, Carbomer 0.1-0.5% by weight, Tocopheryl acetate 0.1-0.5% by weight, Chloroxylenol 0.1-0.5% by weight, 1,2-hexanediol 0.1 -0.5% by weight, chlorphenesin 0.1-0.5% by weight, broccoli leaf/root extract 0.05-0.1% by weight, centella asiatica extract 0.05-0.1% by weight, hydrolyzed glycosaminoglycan 0.1-0.5% by weight, high sodium 0.1-0.5% by weight of alluronate, 0.1-0.5% by weight of sodium hyaluronate crosspolymer, 0.1-0.5% by weight of hydrolyzed hyaluronic acid, 0.1-0.5% by weight of hydroxypropyltrimonium hyaluronate %, benzyl glycol>ceramide NP 0.1-0.5% by weight, sodium acetylated hyaluronate 0.1-0.5% by weight, dipotassium glycyrrhizate 0.05-0.1% by weight, ethylhexylglycerin>polyoxyethylene glyceryl It is characterized in that it contains 0.1-0.5% by weight of monostearate, 0.1-0.5% by weight of citric acid and 0.05-0.1% by weight of fragrance.
또한, 상기 본 발명에 따른 브로콜리 잎 분획물을 이용한 이너뷰티 조성물을 포함하는 크림의 제형으로 제공되는 것을 특징으로 한다.In addition, it is characterized in that it is provided in the formulation of a cream containing the inner beauty composition using the broccoli leaf fraction according to the present invention.
또한, 상술한 목적을 달성하기 위한 본 발명에 따른 브로콜리 잎 분획물을 이용한 이너뷰티 조성물 제조방법은, 최종 조성물 100중량%에 대하여, A로 구분된 정제수 40.0-50.0 중량%, 폴리리신 5.0-10.0 중량%, 글리세린 1.0-5.0 중량%, 펜틸렌글라이콜 1.0-5.0 중량%, 하이드록시프로필메틸셀룰로오스 1.0-5.0 중량%, 나이아신아마이드 1.0-5.0 중량%, 아데노신 0.01-0.05 중량%, 다이소듐이디티에이 0.01-0.05 중량%를 정량하여 메인탱크에 투입하고 용해시키면서 교반시키는 단계; B로 구분된 프로필렌글라이콜다이카프릴레이트/다이카프레이트 10.0-15.0 중량%, 사이클로펜타실록세인 1.0-5.0 중량%, 사이클로헥사실록세인 1.0-5.0 중량%, 세테아릴올리베이트 1.0-5.0 중량%, 솔비탄올리베이트 1.0-5.0 중량%, 카프릴릭/카프릭트라이글리세라이드 1.0-5.0 중량%, 티타늄디옥사이드 0.5-1.0 중량%, 알루미늄하이드록사이드 0.1-0.5 중량%, 트라이에톡시카프릴릴실레인 0.1-0.5 중량%, 다이메티콘 0.1-0.5 중량%, 비닐다이메티콘크로스폴리머 0.5-1.0 중량%, 하이드록시에틸아크릴레이트/소듐아크릴로일다이메틸타우레이트코폴리머 0.5-1.0 중량%, 카보머 0.1-0.5 중량%을 메인탱크에 투입하여 균일하게 혼합된 상기 A와 균일하게 혼합되도록 교반시키는 단계; C로 구분된 토코페릴아세테이트 0.1-0.5 중량%, 클로록실레놀 0.1-0.5 중량%, 1,2-헥산다이올 0.1-0.5 중량%, 클로르페네신 0.1-0.5 중량%을 메인탱크에 투입하여 균일하게 혼합된 상기 A 및 B와 균일하게 혼합되도록 교반시키는 단계; 및 D 및 E로 구분된 브로콜리잎/뿌리추출물 0.05-0.1 중량%, 병풀추출물 0.05-0.1 중량%, 하이드롤라이즈드글라이코사미노글리칸 0.1-0.5 중량%, 소듐하이알루로네이트 0.1-0.5 중량%, 소듐하이알루로네이트크로스폴리머 0.1-0.5 중량%, 하이드롤라이즈드하이알루로닉애씨드 0.1-0.5 중량%, 하이드록시프로필트라이모늄하이알루로네이트 0.1-0.5 중량%, 벤질글라이콜>세라마이드엔피 0.1-0.5 중량%, 소듐아세틸레이티드하이알루로네이트 0.1-0.5 중량%, 다이포타슘글리시리제이트 0.05-0.1 중량%, 에틸헥실글리세린>폴리옥시에칠렌글리세릴모노스테아레이트 0.1-0.5 중량%, 시트릭애씨드 0.1-0.5 중량% 및 향료 0.05-0.1 중량%을 메인탱크에 투입하여 균일하게 혼합된 상기 A, B, C와 균일하게 혼합되도록 교반하는 단계;로 이루어지는 것을 특징으로 한다. In addition, the inner beauty composition manufacturing method using the broccoli leaf fraction according to the present invention for achieving the above object is 40.0-50.0% by weight of purified water classified as A, 5.0-10.0% by weight of polylysine, based on 100% by weight of the final composition %, glycerin 1.0-5.0 wt%, pentylene glycol 1.0-5.0 wt%, hydroxypropylmethylcellulose 1.0-5.0 wt%, niacinamide 1.0-5.0 wt%, adenosine 0.01-0.05% by weight and 0.01-0.05% by weight of disodium EDTA were quantified and introduced into the main tank, and stirring while dissolving; 10.0-15.0% by weight of propylene glycol dicaprylate/dicaprate, 1.0-5.0% by weight of cyclopentasiloxane, 1.0-5.0% by weight of cyclohexasiloxane, 1.0-5.0% by weight of cetearyl olive, classified as B % by weight, sorbitan olivate 1.0-5.0% by weight, caprylic/capric triglyceride 1.0-5.0% by weight, titanium dioxide 0.5-1.0% by weight, aluminum hydroxide 0.1-0.5% by weight, triethoxycapryl Relsilane 0.1-0.5% by weight, dimethicone 0.1-0.5% by weight, vinyl dimethicone crosspolymer 0.5-1.0% by weight, hydroxyethyl acrylate / sodium acryloyldimethyltaurate copolymer 0.5-1.0% by weight %, adding 0.1-0.5% by weight of carbomer to the main tank and stirring to uniformly mix with the uniformly mixed A; 0.1-0.5 wt% of tocopheryl acetate classified as C, 0.1-0.5 wt% of chloroxylenol, 0.1-0.5 wt% of 1,2-hexanediol, and 0.1-0.5 wt% of chlorphenesin were added to the main tank. Stirring to be uniformly mixed with the uniformly mixed A and B; And 0.05-0.1% by weight of broccoli leaf / root extract classified into D and E, 0.05-0.1% by weight of centella asiatica extract, 0.1-0.5% by weight of hydrolyzed glycosaminoglycan, 0.1-0.5% by weight of sodium hyaluronate %, sodium hyaluronate crosspolymer 0.1-0.5% by weight, hydrolyzed hyaluronic acid 0.1-0.5% by weight, hydroxypropyltrimonium hyaluronate 0.1-0.5% by weight, benzyl glycol> Ceramide NP 0.1-0.5% by weight, sodium acetylated hyaluronate 0.1-0.5% by weight, dipotassium glycyrrhizate 0.05-0.1% by weight, ethylhexylglycerin > polyoxyethylene glyceryl monostearate 0.1-0.5% by weight %, adding 0.1-0.5% by weight of citric acid and 0.05-0.1% by weight of perfume to the main tank and stirring them to be uniformly mixed with the uniformly mixed A, B, and C; characterized in that it consists of.
또한, 본 발명에 따른 브로콜리 잎 분획물을 이용한 이너뷰티 조성물 제조방법은, 상기 B를 메인탱크에 투입하여 균일하게 혼합된 상기 A와 균일하게 혼합되도록 교반시키기 전에, 80℃에서 교반기로 균일하게 분산시킨 후 상기 메인탱크에 투입시키는 것을 특징으로 한다. In addition, the method for producing an inner beauty composition using the broccoli leaf fraction according to the present invention is uniformly dispersed with a stirrer at 80 ° C. After that, it is characterized in that it is introduced into the main tank.
또한, 본 발명에 따른 브로콜리 잎 분획물을 이용한 이너뷰티 조성물 제조방법은, 상기 C를 메인탱크에 투입하여 균일하게 혼합된 상기 A 및 B와 균일하게 혼합되도록 교반시키기 전에, 80℃에서 교반기로 균일하게 분산시킨 후 상기 메인탱크에 투입시키는 것을 특징으로 한다.In addition, in the method for producing an inner beauty composition using broccoli leaf fractions according to the present invention, the C is added to the main tank and stirred to be uniformly mixed with the uniformly mixed A and B, uniformly with a stirrer at 80 ° C. After dispersing, it is characterized in that it is introduced into the main tank.
또한, 본 발명에 따른 브로콜리 잎 분획물을 이용한 이너뷰티 조성물 제조방법은, 균일하게 혼합된 상기 A 내지 E의 원료들을 냉각과 동시에 탈포시키고, 이를 1일 숙성시키는 것을 특징으로 한다.In addition, the method for producing an inner beauty composition using a broccoli leaf fraction according to the present invention is characterized in that the uniformly mixed raw materials of A to E are degassed simultaneously with cooling, and aged for one day.
전술한 바와 같이, 본 발명은 항산화 효과 및 산화질소(NO) 생성 억제능을 갖는 브로콜리 잎 분획물을 이용함으로써 미백 및 주름개선에 효과적인 이너뷰티 조성물을 제공하는 효과가 있다.As described above, the present invention has an effect of providing an inner beauty composition effective for whitening and wrinkle improvement by using a broccoli leaf fraction having an antioxidant effect and an ability to inhibit production of nitric oxide (NO).
또한, 본 발명은 브로콜리 잎을 활용한 기능성 소재를 원료화함으로서 농축산물의 비가식 부위를 활용하는 기술개발 연구가 확대될 것으로 기대되며, 이로 인해 버려지는 자원을 활용함으로써 폐기물 감소에 따른 환경개선과 함께 새로운 이익을 창출할 수 있다.In addition, the present invention is expected to expand research on technology development using non-edible parts of agricultural and livestock products by using functional materials using broccoli leaves as raw materials, thereby utilizing discarded resources to improve the environment along with waste reduction. New profits can be created.
또한, 본 발명은 고부가가치를 창출할 수 있는 맞춤형 기능성 화장품산업 육성을 통한 국가 경쟁력 강화와 트렌드에 맞는 맞춤형 화장품의 사업화로 인한 중소기업의 매출 증대 및 고용 증가에 기여할 수 있다. In addition, the present invention can contribute to the increase in sales and employment of small and medium-sized enterprises due to the commercialization of customized cosmetics that meet the trend and strengthen national competitiveness through the development of the customized functional cosmetics industry that can create high added value.
도 1 및 도 2는 브로콜리 잎을 물과 에탄올을 이용하여 추출한 추출물의 항산화 효과 및 산화질소(NO) 생성 억제능을 각각 나타낸 그래프,
도 3은 본 발명에 따른 브로콜리 잎 분획물을 이용한 이너뷰티 조성물의 제조과정을 도식화한 도면,
도 4 내지 도 6은 본 발명에 따른 브로콜리 잎 분획물을 이용한 이너뷰티 조성물의 파라벤 검출시험, 총호기성세균수 검출시험 및 산성도 측정시험 성적서를 각각 나타낸 도면.1 and 2 are graphs showing the antioxidant effect and nitric oxide (NO) production inhibitory ability of the extract obtained by extracting broccoli leaves with water and ethanol, respectively;
Figure 3 is a schematic view of the manufacturing process of the inner beauty composition using the broccoli leaf fraction according to the present invention,
4 to 6 are views showing the results of a paraben detection test, a total aerobic bacterial count detection test, and an acidity measurement test of the inner beauty composition using the broccoli leaf fraction according to the present invention, respectively.
이하에서는 본 발명에 따른 브로콜리 잎 분획물을 이용한 이너뷰티 조성물 및 그 제조방법에 대한 실시 예를 도면을 참조하여 상세히 설명한다. 이하에서 설명되는 실시 예는 통상의 지식을 가진 자에게 발명의 범주를 완전하게 알려주기 위하여 제공되는 것으로, 본 발명은 이하에서 개시되는 실시 예에 한정되지 않고 다양한 형태로 구현될 수 있다. Hereinafter, an embodiment of an inner beauty composition using a broccoli leaf fraction according to the present invention and a manufacturing method thereof will be described in detail with reference to the drawings. The embodiments described below are provided to fully inform those skilled in the art of the scope of the invention, and the present invention may be implemented in various forms without being limited to the embodiments disclosed below.
도면들 중 동일한 구성들은 가능한 한 어느 곳에서든지 동일한 부호들을 나타낸다. 하기의 설명에서 구체적인 특정 사항들이 나타나고 있는데, 이는 본 발명의 보다 전반적인 이해를 돕기 위해 제공된 것일 뿐, 본 발명을 특정한 실시 형태에 대해 한정하려는 것이 아니며, 본 발명의 사상 및 기술 범위에 포함되는 모든 변경, 균등물 내지 대체물을 포함하는 것으로 이해되어야 한다. 그리고 본 발명을 설명함에 있어, 관련된 공지 기능 혹은 구성에 대한 구체적인 설명이 본 발명의 요지를 불필요하게 흐릴 수 있다고 판단되는 경우 그 상세한 설명을 생략한다. Like elements in the drawings indicate like reference numerals wherever possible. Specific details have been shown in the following description, which are only provided to help a more general understanding of the present invention, and are not intended to limit the present invention to specific embodiments, and all changes included in the spirit and technical scope of the present invention , it should be understood to include equivalents or substitutes. In the description of the present invention, if it is determined that a detailed description of a related known function or configuration may unnecessarily obscure the subject matter of the present invention, the detailed description thereof will be omitted.
단수의 표현은 문맥상 명백하게 다르게 뜻하지 않는 한, 복수의 표현을 포함한다. 본 출원에서, "포함하다" 또는 "가지다" 등의 용어는 명세서상에 기재된 특징, 숫자, 단계, 동작, 구성요소, 부품 또는 이들을 조합한 것이 존재함을 지정하려는 것이지, 하나 또는 그 이상의 다른 특징들이나 숫자, 단계, 동작, 구성요소, 부품 또는 이들을 조합한 것들의 존재 또는 부가 가능성을 미리 배제하지 않는 것으로 이해되어야 한다.Singular expressions include plural expressions unless the context clearly dictates otherwise. In this application, the terms "include" or "have" are intended to designate that there is a feature, number, step, operation, component, part, or combination thereof described in the specification, but one or more other features It should be understood that the presence or addition of numbers, steps, operations, components, parts, or combinations thereof is not precluded.
다르게 정의되지 않는 한, 기술적이거나 과학적인 용어를 포함해서 여기서 사용되는 모든 용어들은 본 발명이 속하는 기술 분야에서 통상의 지식을 가진 자에 의해 일반적으로 이해되는 것과 동일한 의미를 가지고 있다. 일반적으로 사용되는 사전에 정의되어 있는 것과 같은 용어들은 본 발명에서의 기능을 고려하여 정의된 것으로서, 이는 본 발명의 기술적 사상에 부합되는 개념과 당해 기술분야에서 통용 또는 통상적으로 인식되는 의미로 해석되어야 하며, 본 출원에서 명백하게 정의하지 않는 한, 이상적이거나 과도하게 형식적인 의미로 해석되지 않는다.Unless defined otherwise, all terms used herein, including technical or scientific terms, have the same meaning as commonly understood by one of ordinary skill in the art to which the present invention belongs. Terms such as those defined in commonly used dictionaries are defined in consideration of functions in the present invention, which should be interpreted as concepts consistent with the technical idea of the present invention and meanings commonly used or commonly recognized in the art. and, unless explicitly defined in this application, are not to be construed in an ideal or overly formal sense.
유럽과 북미의 화장품 시장 특징을 천연물 유래 원료로 고객의 안심감을 강화하고 대표 식물을 육성한 것이라고 한다면, 아시아권의 화장품 시장 특징은 국가별로 약재를 통한 전통 의학과 사상이 결합된 형태로 대내외적인 조화를 이루며 개발되고 있다. 이에 따라, 화장품 원료는 국내의 경우 한의학에 기반을 둠에 따라 동의보감을 토대로 한 한약재를 주로 사용하고, 유럽이나 북미지역의 경우는 허브나 약용 식물을 주로 사용하고 있다.If the characteristics of the cosmetics market in Europe and North America are to strengthen customers’ sense of security and foster representative plants with ingredients derived from natural substances, the characteristics of the cosmetics market in Asia are developed in harmony with internal and external harmony in the form of a combination of traditional medicine and ideology through each country. It is becoming. Accordingly, in Korea, herbal medicines based on Donguibogam are mainly used as raw materials for cosmetics based on oriental medicine, and herbs or medicinal plants are mainly used in Europe and North America.
천연물 유래 제품의 성공을 위해서는 관련 소재의 차별화로 효능 성분을 강화하고 부위별 및 시기별로 신기술 도입을 차별화해 다양한 방법으로 소재를 개발하고, 핵심 성분에 대한 다양한 피부의 효능을 지속적으로 발굴할 수 있는 효능적 차별화와 친환경적인 원료의 재배(생산)를 통한 차별화 전략이 필요하며, 이외에도 환경보전과, 공정무역(fair trade), 그리고 지속가능경영 활동을 통한 사회적 가치를 제고하는 노력이 지속적으로 요구된다. For the success of natural product-derived products, it is necessary to strengthen effective ingredients by differentiating related materials, develop materials in various ways by differentiating the introduction of new technologies by part and time, and continuously discover various skin effects of key ingredients. A differentiation strategy through effective differentiation and the cultivation (production) of eco-friendly raw materials is required, and in addition, continuous efforts are required to enhance social values through environmental preservation, fair trade, and sustainable management activities. .
브로콜리(Broccoli)는 미국 시사주간지 '타임'에서 10대 건강식품으로 선정될 만큼 영양소가 풍부한 채소로서 국내에서의 수요가 급증하면서 재배면적이 증가하는 추세를 나타내고 있다. 국내 주요 재배지역은 제주도와 강원도, 충청북도 순이고, 충청북도에서는 제천과 괴산을 중심으로 재배되고 있고 주로 꽃만 식용하고 잎과 줄기를 버리고 있는 실정이다. Broccoli is a vegetable rich in nutrients enough to be selected as one of the top 10 health foods in the US weekly magazine 'Time'. The major domestic cultivation regions are Jeju-do, Gangwon-do, and Chungcheongbuk-do in order, and in Chungcheongbuk-do, it is cultivated mainly in Jecheon and Goesan.
브로콜리 가식부위의 주요 생리활성물질은 아스코르브산(ascorbic acid), 베타-카로틴(β-carotene), 토코페롤(tocopherol), 루틴(rutin), 셀레늄(selenium), 글루타티온(glutathione), 퀘르세틴(quercetin), 글루코시놀레이츠(glucosinolates) 등이 함유되어 있어 항암, 돌연변이 억제작용, 해독효소의 유도효과, 항산화 활성 등의 기능성이 우수한 것으로 알려져 있다.The main physiologically active substances in broccoli edible parts are ascorbic acid, β-carotene, tocopherol, rutin, selenium, glutathione, quercetin, It contains glucosinolates, etc., and is known to have excellent functions such as anti-cancer, mutation suppression, detoxification enzyme induction, and antioxidant activity.
그러나 잎과 뿌리 등 브로콜리의 비가식 부위에 대해서는 이를 이용한 장류 발효 개발에 관한 보고만 있을 뿐 기능적 생리활성을 다룬 다양한 연구가 미비한 실정이다. However, for non-edible parts of broccoli such as leaves and roots, there are only reports on the development of fermented soy sauce using them, and various studies dealing with functional physiological activity are insufficient.
따라서 본 발명에서는 합동연구를 수행한 연구자(김미경)가 최근 연구에서 브로콜리 잎을 활용한 물과 에탄올 추출물의 피부생리활성 효과를 입증한 결과를 활용함으로써, 충북지역에서 재배되고 있는 농산물인 브로콜리의 비가식 부위인 잎을 용매별로 분획하여 생리활성 성분과 함량 분석 및 피부 생리활성을 효능 검증을 통해 기능성 소재로 개발하였다.Therefore, in the present invention, a researcher (Kim Mi-kyung) who conducted a joint study utilized the results of demonstrating the skin physiological activity effect of water and ethanol extract using broccoli leaves in a recent study, thereby increasing the ratio of broccoli, an agricultural product grown in the Chungbuk region. The leaf, which is the planting site, was fractionated for each solvent, and was developed as a functional material through analysis of physiologically active components and contents and verification of skin physiological activity.
또한, 브로콜리 잎 분획물의 생리활성 효능에 적합한 제형 적용을 통해 기능성 화장품 및 이너뷰티 화장품 개발함에 있어, 분획물의 생리활성 성분의 안정성과 효능을 지속시킬 수 있고, 피부 친화력과 침투력을 증진시킬 수 있는 제형을 적용한 제품을 개발하였다.In addition, in the development of functional cosmetics and inner beauty cosmetics through the application of formulations suitable for the physiological activity of broccoli leaf fractions, formulations capable of maintaining the stability and efficacy of the bioactive components of the fractions and enhancing skin affinity and penetration developed a product using
도 1 및 도 2는 종래기술에 따른 브로콜리 잎을 물과 에탄올을 이용하여 추출한 추출물의 항산화 효과 및 산화질소(NO) 생성 억제능을 각각 나타낸 그래프로서, 도 1의 항산화 효과 그래프에 나타난 바와 같이 IC50 값을 비교하였을 때 물 추출물은 2136.97μg/mL, 에탄올 추출물은 541.33μg/mL로, 브로콜리 잎 에탄올 추출물이 더 높은 소거능을 확인할 수 있고, 도 2의 산화질소 생성 억제능 그래프에 나타난 바와 같이 에탄올 추출물은 농도 500μg/mL에서 36.7%의 산화질소 생성 저해능을 나타내는 것을 확인하였다.1 and 2 are graphs showing the antioxidant effect and nitric oxide (NO) production inhibitory ability of the extract obtained by extracting broccoli leaves using water and ethanol according to the prior art, respectively. As shown in the antioxidant effect graph of FIG. 1, IC 50 When the values were compared, the water extract was 2136.97 μg / mL and the ethanol extract was 541.33 μg / mL, and the higher scavenging ability of the broccoli leaf ethanol extract was confirmed. It was confirmed that at a concentration of 500 μg/mL, 36.7% of nitric oxide production inhibition was exhibited.
하기의 표 1은 브로콜리 잎에 대한 물과 에탄올 추출물의 피부상재균에 대한 항균활성을 대조하여 정리한 표이다. Table 1 below is a table compiled by comparing the antibacterial activities of water and ethanol extracts of broccoli leaves against skin flora.
브로콜리 잎의 용매 분획 제조 및 소재는, 브로콜리 잎 에탄올 추출물을 이용한 용매 분획 방법을 확립하고, 소재의 안정성을 평가한다.For the preparation of the solvent fraction of broccoli leaves and the material, the solvent fractionation method using the ethanol extract of the broccoli leaves was established, and the stability of the material was evaluated.
브로콜리 잎 용매 분획물의 유효성 평가는 전자공여능 및 ABTS 라디컬 소거능 측정을 통해 항산화 활성을 검증하고, 세포 독성 평가, NO 생성 억제능, 염증관련 단백질 발현, 염증반응 지표(TNF-α, IL-4, IL-6, IL-1β) 측정, iNOS와 COX-2 단백질 발현 억제, MAPKs 인산화 억제, NF-κBG활성 억제 측정을 통해 항염증 효능을 평가한다.The efficacy evaluation of the broccoli leaf solvent fraction verifies the antioxidant activity by measuring the electron donating ability and ABTS radical scavenging ability, and the evaluation of cytotoxicity, NO production inhibition ability, expression of inflammation-related proteins, and inflammatory response indicators (TNF-α, IL-4, IL -6, IL-1β) measurement, inhibition of iNOS and COX-2 protein expression, inhibition of MAPKs phosphorylation, and inhibition of NF-κBG activity to evaluate anti-inflammatory efficacy.
또한, 티로시나아제(tyrosinase) 활성 저해와, 세포 독성 평가 및 멜라닌 생성을 평가하여 미백 활성을 평가하고, 세포독성 평가, 콜라주(collage) 생성, MMP-1 생성 평가를 통해 주름 개선 효능을 평가한다.In addition, whitening activity is evaluated by tyrosinase activity inhibition, cytotoxicity evaluation and melanin production evaluation, and wrinkle improvement efficacy is evaluated through cytotoxicity evaluation, collage generation, and MMP-1 production evaluation. .
하기의 표 2는 브로콜리 잎 EA(에틸아세테이트, ethylacetate) 분획물의 인체 피부 자극 정도를 평가하고 이를 통해 피부 안전성 정도를 예측하기 위한 피부 일차 자극 안전성 인체적용시험의 판독 결과를 정리한 표이다.Table 2 below is a table summarizing the reading results of the primary skin irritation safety human application test for evaluating the degree of human skin irritation of the broccoli leaf EA (ethylacetate) fraction and predicting the degree of skin safety therethrough.
표 2에 나타낸 바와 같이, 인체적용시험 결과 브로콜리 잎 EA 분획물의 경우 패치 제거 후 30분, 24시간, 48시간 모두에서 자극이 관찰되지 않았다(패치 제거 30분 후에서 ±가 0명, +이 0명, ++이 0명, +++이 0명 관찰되었으며, 패치 제거 24시간 후에 ±가 0명, +가 0명, ++가 0명, +++가 0명 관찰되었고, 패치 제거 48시간 후에는 ±가 0명, +가 0명, ++가 0명, +++가 0명 관찰되었다). 평균 피부반응도는 0.00으로 피부 첩포시험(Patch Test)의 판정기준에 따라 본 발명에 따른 브로콜리 잎 EA 분획물은 무자극으로 판정되었다. As shown in Table 2, in the case of the broccoli leaf EA fraction as a result of the human application test, no irritation was observed at 30 minutes, 24 hours, and 48 hours after patch removal (30 minutes after patch removal, ± 0 people, + 0 , ++ was observed in 0 persons, +++ was observed in 0 persons, 24 hours after patch removal, ± was observed in 0 persons, + was observed in 0 persons, ++ was observed in 0 persons, +++ was observed in 0 persons, and patch removal 48 After time, ± was observed in 0 patients, + in 0 patients, ++ in 0 patients, and +++ in 0 patients). The average skin reactivity was 0.00, and according to the criterion of the skin patch test, the broccoli leaf EA fraction according to the present invention was determined to be non-irritating.
또한, 브로콜리 잎 EA 분획물의 경우 제품의 특성상 피부 일차 자극 시험에 사용되는 폐쇄첩포시험을 실시할 경우 3가지 최종 판정 기준에서 적합한 것으로 나타났기 때문에 자극 유발 가능성이 낮은 안전한 제품군으로 분류된다.In addition, in the case of the broccoli leaf EA fraction, due to the nature of the product, when the closed patch test used for the primary skin irritation test was performed, it was found to be suitable in the three final criteria, so it is classified as a safe product group with low potential for irritation.
특히, 현재 실시되고 있는 인체 폐쇄첩포시험의 경우, 가혹 조건에서 제품의 안전성을 예측하는 평가 기법이므로, 평가 기준을 모든 화장품에 엄격하게 적용하는 것이 아니라 제품 자체의 사용행태, 특성 등을 고려한 기준에서 평가하는 것이 합당하며, 따라서 본 발명에 따른 브로콜리 잎 EA 분획물의 경우 무자극 제품군으로 판단된다.In particular, in the case of the human body closed patch test currently being conducted, it is an evaluation technique that predicts the safety of products under harsh conditions, so the evaluation criteria are not strictly applied to all cosmetics, but based on the criteria that consider the usage behavior and characteristics of the product itself. It is reasonable to evaluate, and therefore, in the case of the broccoli leaf EA fraction according to the present invention, it is judged as a non-irritant product group.
다음으로, 본 발명에 따른 브로콜리 잎 분획물을 이용한 이너뷰티 조성물의 제조방법을 상세히 설명하고, 이 제조방법에 의해 제조되어 생리활성 성분과 함량 분석 및 피부 생리활성 효능에 적합하고, 피부 친화력과 침투력을 증진시킬 수 있는 제형을 갖는 브로콜리 잎 분획물을 이용한 이너뷰티 조성물을 상세히 설명한다.Next, the manufacturing method of the inner beauty composition using the broccoli leaf fraction according to the present invention will be described in detail, prepared by this manufacturing method, suitable for analysis of physiologically active ingredients and content, and skin physiological activity efficacy, skin affinity and penetration An inner beauty composition using a broccoli leaf fraction having a formulation that can be enhanced will be described in detail.
도 3은 본 발명에 따른 브로콜리 잎 분획물을 이용한 이너뷰티 조성물의 제조과정을 도식화한 도면으로서, 도 3을 참조하여 본 발명에 따른 브로콜리 잎 분획물을 이용한 이너뷰티 조성물의 제조방법을 상세히 설명한다.Figure 3 is a diagram illustrating the manufacturing process of the inner beauty composition using the broccoli leaf fraction according to the present invention, referring to Figure 3 will be described in detail the manufacturing method of the inner beauty composition using the broccoli leaf fraction according to the present invention.
도 3에 도시된 바와 같이 본 발명에 따른 브로콜리 잎 분획물을 이용한 이너뷰티 조성물은 전체 조성물 100중량%에 대하여 A로 구분된 정제수 40.0-50.0 중량%, 폴리리신 5.0-10.0 중량%, 글리세린 1.0-5.0 중량%, 펜틸렌글라이콜 1.0-5.0 중량%, 하이드록시프로필메틸셀룰로오스 1.0-5.0 중량%, 나이아신아마이드 1.0-5.0 중량%, 아데노신 0.01-0.05 중량%, 다이소듐이디티에이 0.01-0.05 중량%를 정량하여 메인탱크에 투입하고 용해시키면서(10) 교반시킨다(11).As shown in FIG. 3, the inner beauty composition using the broccoli leaf fraction according to the present invention contains 40.0-50.0% by weight of purified water classified as A, 5.0-10.0% by weight of polylysine, glycerin with respect to 100% by weight of the total composition. 1.0-5.0 wt%, pentylene glycol 1.0-5.0 wt%, hydroxypropylmethylcellulose 1.0-5.0 wt%, niacinamide 1.0-5.0 wt%, adenosine 0.01-0.05% by weight and 0.01-0.05% by weight of disodium EDTA are quantified and put into the main tank and stirred while dissolving (10) (11).
다음으로, B로 구분된 프로필렌글라이콜다이카프릴레이트/다이카프레이트 10.0-15.0 중량%, 사이클로펜타실록세인 1.0-5.0 중량%, 사이클로헥사실록세인 1.0-5.0 중량%, 세테아릴올리베이트 1.0-5.0 중량%, 솔비탄올리베이트 1.0-5.0 중량%, 카프릴릭/카프릭트라이글리세라이드 1.0-5.0 중량%, 티타늄디옥사이드 0.5-1.0 중량%, 알루미늄하이드록사이드 0.1-0.5 중량%, 트라이에톡시카프릴릴실레인 0.1-0.5 중량%, 다이메티콘 0.1-0.5 중량%, 비닐다이메티콘크로스폴리머 0.5-1.0 중량%, 하이드록시에틸아크릴레이트/소듐아크릴로일다이메틸타우레이트코폴리머 0.5-1.0 중량%, 카보머 0.1-0.5 중량%을 80℃에서 교반기(Stirrer Mixer)로 균일하게 분산시킨 후(20), 메인탱크에 투입하여 균일하게 혼합된 A와 균일하게 혼합되도록 교반한다(21).Next, 10.0-15.0% by weight of propylene glycol dicaprylate/dicaprate, 1.0-5.0% by weight of cyclopentasiloxane, 1.0-5.0% by weight of cyclohexasiloxane, and cetearyl olivate, classified as B 1.0-5.0 wt%, sorbitan olivate 1.0-5.0 wt%, caprylic/capric triglyceride 1.0-5.0 wt%, titanium dioxide 0.5-1.0 wt%, aluminum hydroxide 0.1-0.5 wt%, trier 0.1-0.5% by weight of toxycaprylylsilane, 0.1-0.5% by weight of dimethicone, 0.5-1.0% by weight of vinyl dimethicone crosspolymer, 0.5% by weight of hydroxyethyl acrylate/sodium acryloyldimethyltaurate copolymer After uniformly dispersing -1.0% by weight and 0.1-0.5% by weight of carbomer with a stirrer mixer at 80 ° C (20), it is put into the main tank and stirred so that it is uniformly mixed with the uniformly mixed A (21). ).
다음으로, C로 구분된 토코페릴아세테이트 0.1-0.5 중량%, 클로록실레놀 0.1-0.5 중량%, 1,2-헥산다이올 0.1-0.5 중량%, 클로르페네신 0.1-0.5 중량%을 80℃에서 교반기(Stirrer Mixer)로 균일하게 분산시킨 후(30), 메인탱크에 투입하여 균일하게 혼합된 A 및 B와 균일하게 혼합되도록 교반한다(31).Next, 0.1-0.5% by weight of C-separated tocopheryl acetate, 0.1-0.5% by weight of chloroxylenol, 0.1-0.5% by weight of 1,2-hexanediol, and 0.1-0.5% by weight of chlorphenesin at 80 ° C. After uniformly dispersing with a stirrer mixer (30), it is put into the main tank and stirred to be uniformly mixed with the uniformly mixed A and B (31).
마지막으로, D 및 E로 구분된 브로콜리잎/뿌리추출물 0.05-0.1 중량%, 병풀추출물 0.05-0.1 중량%, 하이드롤라이즈드글라이코사미노글리칸 0.1-0.5 중량%, 소듐하이알루로네이트 0.1-0.5 중량%, 소듐하이알루로네이트크로스폴리머 0.1-0.5 중량%, 하이드롤라이즈드하이알루로닉애씨드 0.1-0.5 중량%, 하이드록시프로필트라이모늄하이알루로네이트 0.1-0.5 중량%, 벤질글라이콜>세라마이드엔피 0.1-0.5 중량%, 소듐아세틸레이티드하이알루로네이트 0.1-0.5 중량%, 다이포타슘글리시리제이트 0.05-0.1 중량%, 에틸헥실글리세린>폴리옥시에칠렌글리세릴모노스테아레이트 0.1-0.5 중량%, 시트릭애씨드 0.1-0.5 중량% 및 향료 0.05-0.1 중량%을 메인탱크에 투입하여 균일하게 혼합된 A, B, C와 균일하게 혼합되도록 교반한다(40).Finally, 0.05-0.1% by weight of broccoli leaf/root extract classified as D and E, 0.05-0.1% by weight of centella asiatica extract, 0.1-0.5% by weight of hydrolyzed glycosaminoglycan, 0.1-0.5% by weight of sodium hyaluronate 0.5% by weight, 0.1-0.5% by weight of sodium hyaluronate crosspolymer, 0.1-0.5% by weight of hydrolyzed hyaluronic acid, 0.1-0.5% by weight of hydroxypropyltrimonium hyaluronate, benzyl glycol Col> Ceramide NP 0.1-0.5% by weight, sodium acetylated hyaluronate 0.1-0.5% by weight, dipotassium glycyrrhizate 0.05-0.1% by weight, ethylhexylglycerin> polyoxyethylene glyceryl monostearate 0.1- 0.5% by weight, 0.1-0.5% by weight of citric acid, and 0.05-0.1% by weight of perfume were added to the main tank and stirred to evenly mix with the uniformly mixed A, B, and C (40).
이후, 상기 40단계에서 균일하게 혼합된 A 내지 E의 원료들을 냉각과 동시에 탈포시키고(50), 이를 1일 숙성시켜(60), 물성치를 측정하여 합격되면 충전(70)하고, 포장 및 보관한다(80). 상기 물성치 측정은 점도, 산도(pH), 비중, 향취, 색상, 함량시험, 확인시험 등을 포함한다. Thereafter, the raw materials of A to E uniformly mixed in
본 발명에 따른 브로콜리 잎 분획물을 이용한 이너뷰티 조성물은 다양한 형태의 제형으로 제조될 수 있으며, 일반 화장품과 달리 식품을 원료로 하여 장기 사용 시 발생할 수 있는 부작용 등이 없고 얼굴을 밝게 톤업시켜 주며 미백 및 주름개선에 효능이 있다. The inner beauty composition using the broccoli leaf fraction according to the present invention can be prepared in various types of formulations, and unlike general cosmetics, it uses food as a raw material and has no side effects that may occur during long-term use, brightens the face, and brightens and brightens the skin. It is effective in improving wrinkles.
본 발명에 따른 브로콜리 잎 분획물을 이용한 이너뷰티 조성물의 처방률 설계를 위해 제형 적합성 시험을 실시하였다. 제형 적합성 시험은 45℃에서 상분리, 변색 및 변취의 안정도를 관찰하였고, 제형 안정도는 4주 동안의 안정도를 관찰하여 상분리, 변색 및 변취에 이상이 없음을 확인하였다. A formulation suitability test was conducted to design the prescription rate of the inner beauty composition using the broccoli leaf fraction according to the present invention. In the formulation suitability test, the stability of phase separation, discoloration, and odor change was observed at 45° C., and the stability of the formulation was observed for 4 weeks to confirm that there was no abnormality in phase separation, discoloration, and odor change.
또한, 본 발명에 따른 브로콜리 잎 분획물을 이용한 이너뷰티 조성물의 시제품 제작 전 보존력(방부력) 시험을 수행하였다. In addition, a preservative force (preservative force) test was performed before manufacturing a prototype of the inner beauty composition using the broccoli leaf fraction according to the present invention.
시험균주는 Guideline에서 제시하는 E. coli (Gram negative), S. aureus (Gram positive), P. aeruginosa (Gram negative), C. albicans (Yeast) 및 A. brasiliensis (Mould)를 사용하였다. 시료에 접종균액을 접종하고 균일하게 혼합한 후 차광상태의 실온에서 보관하고, 보관 후 7일, 14일, 21일, 28일째에 생균수를 측정하였다. 하기의 표 3에 보존력 시험결과를 정리하였다.Test strains are E. coli (Gram negative), S. aureus (Gram positive), P. aeruginosa (Gram negative), C. albicans presented in the Guideline. (Yeast) and A. brasiliensis (Mould) were used. Samples were inoculated with the inoculum solution, mixed uniformly, stored at room temperature in a shaded state, and viable cell counts were measured on
보존력 시험결과, 본 발명에 따른 브로콜리 잎 분획물을 이용한 이너뷰티 조성물은 E. coli , S. aureus, P. aeruginosa , C. albicans, A. brasiliensis 에 대하여 7일, 14일, 21일, 28일 후 모두 99.9 % 이상의 감소율을 나타내었으므로, 보존력이 매우 우수한 것으로 판단된다.As a result of the retention test, the inner beauty composition using the broccoli leaf fraction according to the present invention was effective against E. coli, S. aureus, P. aeruginosa, C. albicans, and A. brasiliensis after 7 days, 14 days, 21 days, and 28 days. Since all of them showed a reduction rate of 99.9% or more, it is judged that the preservation ability is very excellent.
도 4 내지 도 6은 본 발명에 따른 브로콜리 잎 분획물을 이용한 이너뷰티 조성물의 파라벤 검출시험, 총호기성세균수 검출시험 및 산성도 측정시험 성적서를 각각 나타낸 도면이다. 4 to 6 are views showing the results of a paraben detection test, a total aerobic bacterial count detection test, and an acidity measurement test of the inner beauty composition using the broccoli leaf fraction according to the present invention, respectively.
먼저, 본 발명에 따른 브로콜리 잎 분획물을 이용한 이너뷰티 조성물은 화장품이나 의약품의 방부제로 주로 사용되는 파라하이드록시벤조산의 에스터로서 유해성 논란이 있는 파라벤(Paraben)의 검출시험을 실시하였고, 도 4에 나타난 바와 같이 같이 모든 파라벤이 불검출되었다.First, the inner beauty composition using the broccoli leaf fraction according to the present invention was subjected to a detection test of paraben, which is controversial in harmfulness as an ester of parahydroxybenzoic acid, which is mainly used as a preservative for cosmetics or pharmaceuticals, and shown in FIG. As shown, all parabens were not detected.
다음으로, 본 발명에 따른 브로콜리 잎 분획물을 이용한 이너뷰티 조성물은 도 5의 총호기성세균수 검출시험에서 20미만의 세균수와 도 6의 산성도 측정시험에서 pH6.8 산도로, 식약의약품안전처 고시에 적합한 결과를 나타내었다.Next, the inner beauty composition using the broccoli leaf fraction according to the present invention has a bacterial count of less than 20 in the total aerobic bacterial count detection test of FIG. 5 and pH 6.8 acidity in the acidity measurement test of FIG. showed suitable results.
하기의 표 4는 브로콜리 잎 분획물을 이용한 이너뷰티 조성물의 인체 피부 자극 정도를 평가하고 이를 통해 피부 안전성 정도를 예측하기 위한 피부 일차 자극 안전성 인체적용시험의 판독 결과를 정리한 표이다.Table 4 below is a table summarizing the reading results of the primary skin irritation safety human application test to evaluate the degree of human skin irritation of the inner beauty composition using the broccoli leaf fraction and predict the degree of skin safety through this.
화장품의 일차 피부 자극 반응을 평가하는 단회첩포시험은 연구자의 성향이나 시험물질의 특성 등에 따라 24시간 혹은 48시간 첩포 중에서 선택하여 시험할 수 있다. 본 인체적용시험의 경우 24시간 패치 이후 3차에 걸친 판독과정으로 자극 반응을 평가하였다. 24시간 패치의 경우 일반적으로 2회 판독을 실시하나 본 시험의 경우 반응의 강도 추이를 반복적이고 지속적으로 관찰하여 Acute & delayed type의 자극과 감작을 평가함으로 단회첩포시험이 가지는 기술적 한계를 극복하기 위해 총 3회 판독을 선택하여 평가하였다. 본 시험에 사용된 방법은 단회밀폐첩포시험으로 피부 자극 반응을 유발하는 패치부착단계와 피부 자극 및 감작을 전문가에 의한 육안 평가 단계로 구분하여 시험을 수행하였다.The single patch test, which evaluates the primary skin irritation response of cosmetics, can be tested by selecting either 24-hour or 48-hour patch depending on the researcher's tendency or the characteristics of the test substance. In the case of this human application test, the stimulation response was evaluated through three readings after the 24-hour patch. In the case of a 24-hour patch, reading is generally performed twice, but in this test, the change in response intensity is repeatedly and continuously observed to evaluate stimulation and sensitization of the acute & delayed type to overcome the technical limitations of the single-dose patch test. A total of three readings were selected for evaluation. The method used in this test was a single-time closed patch test, and the test was conducted by dividing the patch attachment step that causes skin irritation reaction and the visual evaluation step by experts for skin irritation and sensitization.
표 4에 나타낸 바와 같이, 인체적용시험 결과 브로콜리 잎 분획물을 이용한 이너뷰티 조성물의 경우 패치 제거 후 30분, 24시간, 48시간 모두에서 자극이 관찰되지 않았다(패치 제거 30분 후에서 ±가 0명, +이 0명, ++이 0명, +++이 0명 관찰되었으며, 패치 제거 24시간 후에 ±가 0명, +가 0명, ++가 0명, +++가 0명 관찰되었고, 패치 제거 48시간 후에는 ±가 0명, +가 0명, ++가 0명, +++가 0명 관찰되었다). 평균 피부반응도는 0.00으로 피부 첩포시험(Patch Test)의 판정기준에 따라 브로콜리 잎 분획물을 이용한 이너뷰티 조성물은 무자극으로 판정되었다.As shown in Table 4, in the case of the inner beauty composition using the broccoli leaf fraction as a result of the human application test, no irritation was observed at 30 minutes, 24 hours, and 48 hours after patch removal (± 0
또한, 본 발명에 따른 브로콜리 잎 분획물을 이용한 이너뷰티 조성물의 경우 제품의 특성상 피부 일차 자극 시험에 사용되는 폐쇄첩포시험을 실시할 경우 3가지 최종 판정 기준에서 적합한 것으로 나타났기 때문에 자극 유발 가능성이 낮은 안전한 제품군으로 분류할 수 있다.In addition, in the case of the inner beauty composition using the broccoli leaf fraction according to the present invention, when the closed patch test used for the primary skin irritation test was performed due to the nature of the product, it was found to be suitable in the three final criteria, so it is a safe product group with low possibility of irritation can be classified as
현재 실시되고 있는 인체 폐쇄첩포시험의 경우, 가혹 조건에서 제품의 안전성을 예측하는 평가 기법이므로, 평가 기준을 모든 화장품에 엄격하게 적용하는 것이 아니라 제품 자체의 사용행태, 특성 등을 고려한 기준에서 평가하는 것이 합당하며, 이에 따라 본 발명에 따른 브로콜리 잎 분획물을 이용한 이너뷰티 조성물은 무자극 제품군으로 인정된다. In the case of the human body closed patch test currently being conducted, it is an evaluation technique that predicts the safety of products under harsh conditions, so the evaluation criteria are not strictly applied to all cosmetics, but evaluated based on the criteria that consider the usage behavior and characteristics of the product itself. It is reasonable, and accordingly, the inner beauty composition using the broccoli leaf fraction according to the present invention is recognized as a non-irritant product group.
전술한 바와 같이, 본 발명에 따른 브로콜리 잎 분획물을 이용한 이너뷰티 조성물 및 그 제조방법은 브로콜리 잎을 활용한 기능성 소재를 원료화함으로서 농축산물의 비가식 부위를 활용하는 기술개발 연구가 확대될 것으로 기대되며, 이로 인해 버려지는 자원을 활용함으로써 폐기물 감소에 따른 환경개선과 함께 새로운 이익을 창출할 수 있고, 고부가가치를 창출할 수 있는 맞춤형 기능성 화장품산업 육성을 통한 국가 경쟁력 강화와 트렌드에 맞는 맞춤형 화장품의 사업화로 인한 중소기업의 매출 증대 및 고용 증가에 기여할 수 있다. As described above, the inner beauty composition and method of manufacturing the inner beauty composition using the broccoli leaf fraction according to the present invention is expected to expand technology development research utilizing non-edible parts of agricultural and livestock products by using functional materials using broccoli leaves as raw materials. , By using discarded resources, new profits can be created along with environmental improvement due to waste reduction, and commercialization of customized cosmetics that meet trends and strengthen national competitiveness through fostering of the customized functional cosmetics industry that can create high added value. As a result, it can contribute to the increase in sales and employment of SMEs.
한편, 본 발명의 상세한 설명에서는 첨부된 도면에 의해 참조되는 바람직한 실시 예를 중심으로 구체적으로 기술되었으나, 본 발명의 범위에서 벗어나지 않는 한도 내에서 여러 가지 변형이 가능함은 물론이다. 그러므로 본 발명의 범위는 설명된 실시 예에 국한되어 정해져서는 안되며 후술하는 특허청구의 범위뿐 아니라 이 특허청구의 범위와 균등한 것들에 의해서 정해져야 한다.Meanwhile, in the detailed description of the present invention, although it has been specifically described with reference to the preferred embodiments referenced by the accompanying drawings, various modifications are possible without departing from the scope of the present invention. Therefore, the scope of the present invention should not be limited to the described embodiments and should not be defined, but should be defined by not only the scope of the claims to be described later, but also those equivalent to the scope of these claims.
Claims (5)
Based on the total 100% by weight, 40.0-50.0% by weight of purified water, 5.0-10.0% by weight of polylysine, 1.0-5.0% by weight of glycerin, 1.0-5.0% by weight of pentylene glycol, 1.0-5.0% by weight of hydroxypropylmethylcellulose, Niacinamide 1.0-5.0% by weight, adenosine 0.01-0.05% by weight, disodium EDTA 0.01-0.05% by weight, propylene glycol dicaprylate/dicaprate 10.0-15.0% by weight, cyclopentasiloxane 1.0- 5.0 wt%, cyclohexasiloxane 1.0-5.0 wt%, cetearyl olive 1.0-5.0 wt%, sorbitan olivate 1.0-5.0 wt%, caprylic/capric triglyceride 1.0-5.0 wt%, titanium Dioxide 0.5-1.0% by weight, aluminum hydroxide 0.1-0.5% by weight, triethoxycaprylylsilane 0.1-0.5% by weight, dimethicone 0.1-0.5% by weight, vinyldimethicone crosspolymer 0.5-1.0% by weight %, hydroxyethyl acrylate/sodium acryloyldimethyltaurate copolymer 0.5-1.0% by weight, carbomer 0.1-0.5% by weight, tocopheryl acetate 0.1-0.5% by weight, chloroxylenol 0.1-0.5% by weight , 0.1-0.5% by weight of 1,2-hexanediol, 0.1-0.5% by weight of chlorphenesin, 0.05-0.1% by weight of broccoli leaf/root extract, 0.05-0.1% by weight of centella asiatica extract, hydrolyzed glycosaminoglyc Khan 0.1-0.5% by weight, sodium hyaluronate 0.1-0.5% by weight, sodium hyaluronate crosspolymer 0.1-0.5% by weight, hydrolyzed hyaluronic acid 0.1-0.5% by weight, hydroxypropyltri 0.1-0.5% by weight of monium hyaluronate, 0.1-0.5% by weight of benzyl glycol>ceramide NP, 0.1-0.5% by weight of sodium acetylated hyaluronate, 0.05-0.1% by weight of dipotassium glycyrrhizate, An inner beauty composition using a broccoli leaf fraction containing 0.1-0.5% by weight of ethylhexylglycerin> polyoxyethylene glyceryl monostearate, 0.1-0.5% by weight of citric acid, and 0.05-0.1% by weight of fragrance.
A cream containing an inner beauty composition using the broccoli leaf fraction of claim 1.
B로 구분된 프로필렌글라이콜다이카프릴레이트/다이카프레이트 10.0-15.0 중량%, 사이클로펜타실록세인 1.0-5.0 중량%, 사이클로헥사실록세인 1.0-5.0 중량%, 세테아릴올리베이트 1.0-5.0 중량%, 솔비탄올리베이트 1.0-5.0 중량%, 카프릴릭/카프릭트라이글리세라이드 1.0-5.0 중량%, 티타늄디옥사이드 0.5-1.0 중량%, 알루미늄하이드록사이드 0.1-0.5 중량%, 트라이에톡시카프릴릴실레인 0.1-0.5 중량%, 다이메티콘 0.1-0.5 중량%, 비닐다이메티콘크로스폴리머 0.5-1.0 중량%, 하이드록시에틸아크릴레이트/소듐아크릴로일다이메틸타우레이트코폴리머 0.5-1.0 중량%, 카보머 0.1-0.5 중량%을 메인탱크에 투입하여 균일하게 혼합된 상기 A와 균일하게 혼합되도록 교반시키는 단계;
C로 구분된 토코페릴아세테이트 0.1-0.5 중량%, 클로록실레놀 0.1-0.5 중량%, 1,2-헥산다이올 0.1-0.5 중량%, 클로르페네신 0.1-0.5 중량%을 메인탱크에 투입하여 균일하게 혼합된 상기 A 및 B와 균일하게 혼합되도록 교반시키는 단계; 및
D 및 E로 구분된 브로콜리잎/뿌리추출물 0.05-0.1 중량%, 병풀추출물 0.05-0.1 중량%, 하이드롤라이즈드글라이코사미노글리칸 0.1-0.5 중량%, 소듐하이알루로네이트 0.1-0.5 중량%, 소듐하이알루로네이트크로스폴리머 0.1-0.5 중량%, 하이드롤라이즈드하이알루로닉애씨드 0.1-0.5 중량%, 하이드록시프로필트라이모늄하이알루로네이트 0.1-0.5 중량%, 벤질글라이콜>세라마이드엔피 0.1-0.5 중량%, 소듐아세틸레이티드하이알루로네이트 0.1-0.5 중량%, 다이포타슘글리시리제이트 0.05-0.1 중량%, 에틸헥실글리세린>폴리옥시에칠렌글리세릴모노스테아레이트 0.1-0.5 중량%, 시트릭애씨드 0.1-0.5 중량% 및 향료 0.05-0.1 중량%을 메인탱크에 투입하여 균일하게 혼합된 상기 A, B, C와 균일하게 혼합되도록 교반하는 단계;로 이루어지는 것을 특징으로 하는 브로콜리 잎 분획물을 이용한 이너뷰티 조성물 제조방법.
Based on 100% by weight of the final composition, 40.0-50.0% by weight of purified water classified as A, 5.0-10.0% by weight of polylysine, 1.0-5.0% by weight of glycerin, 1.0-5.0% by weight of pentylene glycol, 1.0% by weight of hydroxypropylmethylcellulose -5.0% by weight, niacinamide 1.0-5.0% by weight, adenosine 0.01-0.05% by weight, disodium EDTA 0.01-0.05% by weight were weighed and put into the main tank and stirred while dissolving;
10.0-15.0% by weight of propylene glycol dicaprylate/dicaprate, 1.0-5.0% by weight of cyclopentasiloxane, 1.0-5.0% by weight of cyclohexasiloxane, 1.0-5.0% by weight of cetearyl olive, classified as B % by weight, sorbitan olivate 1.0-5.0% by weight, caprylic/capric triglyceride 1.0-5.0% by weight, titanium dioxide 0.5-1.0% by weight, aluminum hydroxide 0.1-0.5% by weight, triethoxycapryl Relsilane 0.1-0.5% by weight, dimethicone 0.1-0.5% by weight, vinyl dimethicone crosspolymer 0.5-1.0% by weight, hydroxyethyl acrylate / sodium acryloyldimethyltaurate copolymer 0.5-1.0% by weight %, adding 0.1-0.5% by weight of carbomer to the main tank and stirring to uniformly mix with the uniformly mixed A;
0.1-0.5 wt% of tocopheryl acetate classified as C, 0.1-0.5 wt% of chloroxylenol, 0.1-0.5 wt% of 1,2-hexanediol, and 0.1-0.5 wt% of chlorphenesin were added to the main tank. Stirring to be uniformly mixed with the uniformly mixed A and B; and
0.05-0.1% by weight of broccoli leaf/root extract classified as D and E, 0.05-0.1% by weight of centella asiatica extract, 0.1-0.5% by weight of hydrolyzed glycosaminoglycan, 0.1-0.5% by weight of sodium hyaluronate , sodium hyaluronate crosspolymer 0.1-0.5% by weight, hydrolyzed hyaluronic acid 0.1-0.5% by weight, hydroxypropyltrimonium hyaluronate 0.1-0.5% by weight, benzyl glycol>ceramide NP 0.1-0.5% by weight, sodium acetylated hyaluronate 0.1-0.5% by weight, dipotassium glycyrrhizate 0.05-0.1% by weight, ethylhexylglycerin > polyoxyethylene glyceryl monostearate 0.1-0.5% by weight , Adding 0.1-0.5% by weight of citric acid and 0.05-0.1% by weight of flavorings to the main tank and stirring them to be uniformly mixed with the uniformly mixed A, B, and C; Broccoli leaf fraction, characterized in that it consists of Inner beauty composition manufacturing method using.
상기 B는,
메인탱크에 투입하여 균일하게 혼합된 상기 A와 균일하게 혼합되도록 교반시키기 전에 80℃에서 교반기로 균일하게 분산시킨 후 상기 메인탱크에 투입시키고,
상기 C는,
메인탱크에 투입하여 균일하게 혼합된 상기 A 및 B와 균일하게 혼합되도록 교반시키기 전에 80℃에서 교반기로 균일하게 분산시킨 후 상기 메인탱크에 투입시키는 것을 특징으로 하는 브로콜리 잎 분획물을 이용한 이너뷰티 조성물 제조방법.
According to claim 3,
The B is,
Before being put into the main tank and stirred to be uniformly mixed with the uniformly mixed A, uniformly dispersed with a stirrer at 80 ° C, and then put into the main tank,
The C is,
Inner beauty composition using broccoli leaf fraction, characterized in that it is added to the main tank and uniformly dispersed with a stirrer at 80 ° C. before stirring to uniformly mix with the uniformly mixed A and B, and then introduced into the main tank. method.
균일하게 혼합된 상기 A 내지 E의 원료들을 냉각과 동시에 탈포시키고, 이를 1일 숙성시키는 것을 특징으로 하는 브로콜리 잎 분획물을 이용한 이너뷰티 조성물 제조방법.According to claim 3 or 4,
Inner beauty composition manufacturing method using broccoli leaf fraction, characterized in that the uniformly mixed raw materials of A to E are cooled and degassed at the same time, and aged for one day.
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KR101729913B1 (en) | 2014-04-14 | 2017-04-25 | 주식회사 비케이바이오 | Preparation method for broccoli with an increased sulforaphane content and the use of it |
KR101725150B1 (en) | 2015-08-24 | 2017-04-10 | 경상대학교산학협력단 | Composition for improvement of learning and memory function comprising broccoli leaf extract or its fraction as effective component |
KR102119795B1 (en) | 2018-07-24 | 2020-06-05 | 서원대학교산학협력단 | Anti-inflammatory Composition Comprising Broccoli Extract |
KR20200106296A (en) | 2019-03-04 | 2020-09-14 | 주식회사 비케이바이오 | Composition for improvement of memory or cognitive ability comprising broccoli sprouts |
KR20210012394A (en) | 2019-07-25 | 2021-02-03 | 주식회사 비케이바이오 | A composition for enhancing immunity comprising broccoli leaves |
KR102301757B1 (en) | 2020-12-18 | 2021-09-13 | 서울대학교산학협력단 | Natural composition suppressing a stress-induced memory loss and improving learning ability |
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