KR20230041494A - Method of controlling Thrips using Beauveria bassiana KNU-101 strain-derived microbial agent - Google Patents

Abstract

According to the present invention, provided is a method for controlling thrips, wherein the method comprises: a soil spraying step of spraying a microbial agent for killing thrips derived from a Beauveria bassiana KNU-101 strain (accession number KCTC 13660BP) on soil where crops will be planted in an amount of 100 to 300 g/10a based on an active ingredient; and a drench treatment step of treating the soil with the microbial agent diluted in water in an amount of 100 to 300 g/10a based on the active ingredient while the crop is in a planted state. Therefore, high control efficiency against thrips can be obtained.

Description

Method of controlling thrips using Beauveria bassiana KNU-101 strain-derived microbial agent {Method of controlling Thrips using Beauveria bassiana KNU-101 strain-derived microbial agent}

The present invention relates to a control method for thrips, and more particularly, to a control method for thrips that can effectively control thrips using a microbial agent derived from Boveria bassiana KNU-101 strain having a control effect against thrips It is about prevention methods.

Chemical insecticides, which have been used in large quantities to control insects harmful to agricultural crops, such as pests, eradicate non-target microorganisms such as beneficial insects or parasites of pests due to their non-specificity in insecticidal ability, and pests caused by repeated use of chemical insecticides Disadvantages such as resistance acquisition and harmfulness to the human body have been pointed out as problems.

On the other hand, Beauveria bassiana is known as a representative insect pathogenic fungus, and its spores decompose the wax layer of insects, leading to death by dehydrating insects and feeding their dead bodies to the next generation. It is known. For this reason, it is known that both oral and contact toxicities to insects are effective and that various insect hosts can be infected. Solid culture for producing conidia is mainly used for mass cultivation of spores of fungi for controlling pests.

However, unlike bacteria, fungi have insufficient spore production despite a long-term cultivation period of at least 7 days, so the commercialization of fungi for pest control is delayed. It is known to be at a low level for use as a Therefore, for industrialization, it is a priority task to select a fungus that has both requirements for producing a large amount of spores while exhibiting excellent insecticidal activity.

Korean Patent Registration No. 10-2251508 discloses Beauveria bassiana, a new insect pathogenic fungus that meets the above requirements. bassiana ) KNU-101 strain (accession number KCTC 13660BP) and a microbial agent for pest control using the same are disclosed.

The new strain has a thrips lethal effect and remarkable spore-producing ability, and can be mass-produced, so that an insecticidal microbial preparation including active ingredients is prepared from the culture of the strain, and treated with soil or plants to treat pests. In particular, it is known that it can be used for insecticide against thrips insects such as cucumber thrips and yellow flower thrips.

On the other hand, Thrips, a representative soil pest, lay eggs on soft tissues such as young leaves, stems, and petals, and begin to appear as the temperature rises, damaging the surface of stems and leaves, and damaging flower buds during the flowering period. It seriously reduces crop quality. Damaged crops do not straighten out their leaves and show deformed lesions or contraction of leaf veins. Thrips have a wide range of hosts, such as cucumber, lettuce, tomato, eggplant, and chicory, and have a very short generation period of at least 12 days to a maximum of 26 days depending on temperature conditions, and are difficult to control because they have strong fertility.

In particular, thrips like yellow and white colors, so when flowers such as cucumbers and peppers bloom, they attack the flowers and damage the young fruits. Therefore, the damaged fruit becomes a deformed fruit as it grows, or many gray or brown messy scars are left on the skin of the fruit, resulting in poor marketability.

Cucumber thrips (Thrips palmi Karrny), yellow flower thrips (Frankliniella occidentalis (Pergande)), and Taiwanese thrips (Frankliniella intonsa (Trybom)) are representative types of thrips that damage cucumbers and peppers.

On the other hand, adult thrips spawn mainly in the leaf veins on the back of the leaves, so the hatched nymphs attack along the leaf veins, and the damaged area initially becomes white, but gradually turns brown, and in severe cases, the entire leaf dries up and dies. When exposed to the sunlight, the damaged area has a white luster. When the fruit is damaged, the damaged area turns brown and a cork layer is formed, and the surface turns brown and becomes rough. In particular, in cucumbers, a lot of cereals and deformed fruits are formed, which makes them of no commercial value.

The adult lifespan is about 37 days at 20℃ and 28 days at 25℃, and it reproduces quickly because it has both bisexual and parthenogenesis, and each development form coexists in multiple areas. Adults lay eggs one by one in tissues such as fruit stems, calyxes, veins, petioles, and mesophyll of plants, and one adult lays about 100 eggs. The egg period is about 4-5 days. When the hatched larva passes the second instar and becomes a homeless larva, it falls to the ground and becomes the 1st and 2nd pupae in the soil at a depth of 2-3cm in the ground, under fallen leaves, or under vinyl, etc., and becomes an adult. becomes It takes about 14-18 days at 25℃ to pass one generation, and it does not develop below 11℃ and above 35℃.

As such, since thrips have a short intergenerational interval and survive in various forms on plants and in the ground, even if an insecticidal microbial agent having an insecticidal effect on thrips is used, it is impossible to eradicate thrips existing in each form at once. There is a need to develop a control method that can obtain a high control effect.

In addition, adults of thrips can fly and move, and homeless larvae and pupae fall to the ground as described above and exist in shallow surface soil at a depth of 2-3 cm, and the control solution is diluted in water and injected In the case of this, there is a problem that the control efficiency is lowered because it does not stay on the surface and penetrates deep into the ground.

The present invention has been made to improve the problems in controlling the above-mentioned thrips, and the present invention is effective against the Boveria bassiana KNU-101 strain, its culture, etc. known to have an insecticidal effect on cucumber thrips. It is an object of the present invention to provide an effective control method capable of obtaining a high control effect against thrips throughout the growing period of crops such as cucumbers, while controlling thrips using a microbial agent for killing thrips.

The thrips control method according to the present invention for solving the above problems is,

Beauveria Basiana bassiana ) KNU-101 strain (accession number KCTC 13660BP), a culture of the strain, a concentrate of the culture, and a microbial agent for killing thrips containing at least one selected from the group consisting of dried products of the culture as an active ingredient In the method for controlling thrips using,

A soil spraying step of spraying the microorganism preparation in an amount of 100 ~ 300g / 10a based on the active ingredient in the soil to plant the crop;

It is characterized in that it comprises a drench treatment step of drenching the soil in an amount of 100 to 300 g / 10a based on the active ingredient of the microorganism preparation diluted in water in a state where the crop is planted.

Preferably, a moisturizing soil formation step of spraying 1 to 3 kg/10a of aloe composite dry powder on the soil is performed 1 to 10 days before the soil spreading step,

In addition, preferably, 100 to 500 g / 10a of sugar is mixed with the microbial agent and sprayed together in the soil spraying step or the drenching step,

In addition, in the soil spreading step, 1 to 3 kg/10a of aloe complex dry powder is sprayed together with the microbial agent, or 100 to 500 g/10a of sodium bisethylhexylsulfosuccinate of the microbial agent in the irrigation step. (Sodium bis (2-ethylhexyl) sulfosuccinate) or sodium benzoate (sodium benzoate) is preferably mixed and sprayed together.

According to the control method according to the present invention, since the generation cycle is short and it is possible to continuously kill thrips pests that are difficult to control because they exist in various forms in plants and the ground, high control efficiency against thrips pests can be obtained.

1 is a graph showing the control effect according to the control method in the early stage of crop growth;
Figure 2 is a graph showing the control effect according to the control method in the middle of the growth of crops.

Hereinafter, the method for controlling thrips according to the present invention will be described in detail.

The microbial agent used in the method for controlling thrips pests of the present invention is Beauveria bassiana ( Beauveria bassiana ) KNU-101 strain (accession number KCTC 13660BP), a culture of the strain, a concentrate of the culture, and a dried product of the culture, and at least one selected from the group consisting of an active ingredient.

Beauveria Basiana bassiana ) KNU-101 strain (Accession No. KCTC 13660BP) selects strains that have an insecticidal effect on thrips from thrips, crops and soil where thrips are inhabited and killed by infection with insect pathogenic fungi, and insect Killed insects infected with pathogenic fungi and the surrounding soil were collected to isolate strains having an insecticidal effect on thrips, and strains exhibiting an excellent insecticidal effect on thrips were selected and identified, with a mortality rate of 100% against thrips. It is an insect pathogenic strain with.

In the microbial preparation used in the present invention, additives such as surfactants, weighting agents, nutrients, etc. may be further added to the Beauveria bassiana KNU-101 strain, etc., in the form of granules, hydration agents or encapsulated formulations. It may be manufactured, and in the case of granules, surface active agent, inert carrier, preservative, wetting agent, supply accelerator, attractant, encapsulant binder, emulsifier, dye, UV protection agent, buffer and flow agent, low-flying powder, fine powder, sleep development It may be prepared by including one or more selected from the formulation group consisting of zero.

Beauveria Basiana used in the control method of the present invention ( Beauveria bassiana ) KNU-101 strain (Accession No. KCTC 13660BP) derived from thrips insecticidal microbial preparations and more detailed information on the properties are in accordance with the contents described in Patent Registration No. 10-2251508.

The method for controlling thrips using a microbial preparation derived from the Boveria bassiana KNU-101 strain according to the present invention uses the microbial preparation for killing thrips, and cucumber thrips (thrips) that damage cucumbers or peppers, etc. palmi Karrny), yellow flower thrips (Frankliniella occidentalis (Pergande)), and Taiwan thrips (Frankliniella intonsa (Trybom)).

According to the method of the present invention, the soil spraying step of spraying the microorganism preparation in an amount of 100 ~ 300g / 10a based on the active ingredient in the soil to plant crops; A drench treatment step of drenching the soil in an amount of 100 to 300 g / 10a based on the active ingredient of the microorganism preparation diluted in water in a state where the crop is planted.

In the soil spraying step, before planting crops such as cucumbers, 100 to 300 g/10a of a microbial agent based on active ingredients is sprayed in advance, so that the old larvae or pupae of the caterpillars present in the soil are Boveria bassiana KNU. -101 strain is inoculated so that insecticide is achieved.

Because crops such as cucumbers are mulched with mulching vinyl, etc. before planting, it is often difficult to spread the soil. Therefore, in order to uniformly spray a sufficient amount of the microbial agent, it is necessary to spray the microbial agent to the soil before planting crops. A control effect of more than 50% can be obtained by such soil spraying.

If the amount of the microbial agent used is less than 100g / 10a, the control effect is low, making it difficult to obtain sufficient control effect, and economical efficiency is poor if it is 300g / 10a or more.

At this time, when 100 to 500 g/10a of sugar is mixed with the microbial preparation and sprayed together, it can be a nutrient for the Boveria bassiana KNU-101 strain until it is inoculated and multiplied by thrips.

Preferably, when 1 to 3 kg/10 a of aloe composite dry powder is mixed with a microbial agent and sprayed, soil humidity can be maintained suitable for the growth of the Boveria bassiana KNU-101 strain.

For the aloe complex dried powder according to this embodiment, the collected raw aloe is cut into pieces, and the cut raw aloe is freeze-dried for 36 to 40 hours in a freeze-drying room maintained at a temperature of -20 to -28 ° C. It is made by crushing.

More preferably, the aloe powder that has undergone the primary treatment process including freeze drying and primary crushing is put into a drying oven at 90°C to 95°C for 3 hours and 5 hours, and dried at a high temperature to maintain a moisture content of 3% to 5%. Then, the dried aloe powder is secondarily pulverized to form an aloe complex dry powder having finer particles.

In this way, the aloe composite dry powder, which has completed the first treatment process including freeze-drying and primary grinding and the secondary treatment process including high-temperature drying and secondary crushing, is mixed with sugar and microbial agent and clogged the nozzle when irrigation spraying. In addition to preventing the occurrence, sterilization of bacteria that may exist in raw aloe is achieved, and further improved moisturizing ability is obtained by increasing the surface area by atomization through secondary grinding.

If the amount of the complex dry powder of sugar and aloe used is less than the above range, the effect is insignificant, and if it exceeds the above range, economic feasibility deteriorates. In this way, by using sugar and aloe powder together with a microbial agent, the control effect can be improved by 5 to 10%, respectively.

The pupae of thrips exist in the soil at a shallow depth of 2-3 cm, and since the surface is easy to dry, it is not possible to create an environment suitable for the growth of Boveria bassiana KNU-101 strain, a pest-pathogenic fungus.

In order to solve this problem, in the present embodiment, a moisturizing soil layer forming step of spraying 1 to 3 kg/10a of sugar and aloe complex dry powder to the soil is performed 1 to 10 days before the soil spreading step.

Preferably, when the soil is sprayed, the aloe complex dry powder and sugar are mixed in a ratio of 2:1 and sprayed, and then mulching is performed. It absorbs and forms a moist soil layer at a depth of 2 cm to 3 cm from the soil epidermis, thereby creating an environment suitable for the growth of the Boveria bassiana KNU-101 strain to be drenched later.

Here, the sugar mixed with the aloe complex dry powder and sprayed on the soil is dissolved in a gel state by the moisture absorbed through the aloe complex dry powder, and the moisture-absorbed aloe complex dry powder is deposited at a depth of 2 cm to 3 cm from the soil epidermis. form a moist soil layer.

That is, since it dries easily before covering the mulch vinyl, if you spray the eco-friendly aloe complex dry powder that can absorb and maintain moisture in advance, the soil will not only not dry out, but will also absorb the moisture of the soil overlaid with mulching, making the soil Loss of the Boveria bassiana KNU-101 strain can be prevented by forming a moisture-retaining soil layer with high humidity on the epidermis.

In particular, as a result of the test, it was shown that the soil containing the pupae of the thrips can be formed in a good state for the growth of fungi by spraying the aloe composite dry powder in advance before the soil spraying of the microbial preparation, thereby showing an improved control effect.

In this state, when the microbial agent is sprayed on the soil, it becomes an environment in which the Boveria bassiana KNU-101 strain is easy to grow in the soil where appropriate humidity is formed, and it can exert an insecticidal effect on the homeless larvae or pupae of thrips in the soil. There is an effect that can maintain the improved control effect throughout the growing season of crops.

By going through such a pre-moisturized soil formation step, the control effect has an effect of improving by 10% or more.

In this way, after mulching the ridges of the soil where the microbial agent is sprayed, cucumber crops and the like are planted. This process may be performed according to a conventional method of cultivating crops.

It is necessary to control thrips that alternately exist in the ground and in the above-ground plants by spraying additional microbial agents during the growing season after the crops are planted.

Through the soil spraying step, control against old larvae and pupae of thrips existing in the soil can be achieved, but thrips derived from the outside propagate while changing generations in a cycle of only 14-18 days, and by soil spraying Since it is difficult for the microbial agent supplied to the soil to exert the same control effect throughout the growing season of the crop, additional control is required for homeless larvae and pupae that have fallen to the ground.

Therefore, in the present invention, one or more irrigation treatment steps are performed after the mulching and planting steps.

In the drenching treatment step, the microbial agent diluted in water in the state where the crop is planted is supplied in an amount of 100 to 300 g/10a through a water pipe or the like installed in the soil.

In addition, preferably, 100 to 500 g / 10a of sodium bis (2-ethylhexyl) sulfosuccinate or sodium benzoate in the soil spraying step or drenching treatment step is used as the microbial agent It is sprayed together to increase the control effect of microbial agents.

The sodium bis(2-ethylhexyl) sulfosuccinate and sodium benzoate are added to improve soil moisture retention, are in the form of light yellow powder, and are resistant to water. Because of its good solubility, it can be added to microbial preparations and processed together, especially during irrigation.

Example

Example 1 and comparative example 1 to 3 : Control effect according to treatment method

(1)In the early stage of growth (January to April) control effect

Beauveria Basiana bassiana ) KNU-101 strain (accession number KCTC 13660BP) was used as an active ingredient, and the control effect against thrips was tested by varying the treatment method as shown in Table 1 below.

As for the microbial preparation used, granules with an active ingredient content of 5% were used in the case of soil treatment, and granules with an active ingredient content of 50% were used in the case of irrigation treatment.

The control effect is shown in Table 1 by examining the incidence of thrips in comparison with Comparative Example 1, which is an untreated group, using yellow sticky traps, and the change in the number of thrips captured per actual trap over time is shown in the graph of FIG. 1 .

division processing method processing time Throughput Prevention (%) Comparative Example 1 untreated - - 0 Comparative Example 2 soil spreading january 3kg/10a 50 Comparative Example 3 Soil spraying (agrochemical) + drench treatment January + March 3kg/10a+300g/10a 74.2 Example 1 Soil spray + drench treatment January + March 3kg/10a+300g/10a 71.7

As shown in Table 1, in the case of Example 1 in which soil spraying and drenching treatment were combined according to the method of the present invention, the control value was over 70%, and significantly improved control compared to the case of Comparative Example 2 in which only soil spraying was performed. , which is similar to Comparative Example 3 in which chemical pesticides are sprayed on the soil.

Therefore, it can be seen that even if only microbial agents are sprayed without spraying pesticides on the soil, a high control effect can be obtained by performing additional drenching treatment.

(2) Of the middle and late stages of growth (May-July) control effect

In addition to the treatment of each Example and Comparative Example described in Table 1, additional drenching treatment as shown in Table 2 below and pesticide spraying on crops in the above-ground part were added to test the control effect against thrips in the middle stage of growth. did The treatment contents prior to additional drenching treatment or pesticide spraying are the same as those shown in Table 1 above.

The microbial preparations used are the same as those described above, and in the case of pesticides and pesticides, a commercially available emamectin benzoate emulsion was diluted with water at a magnification of 2,000 times and sprayed at an amount of 300 L/10a based on the diluted amount.

In order to confirm the control effect, the occurrence rate of thrips was investigated in comparison with Comparative Example 1, which is an untreated group, using similarly yellow sticky traps, and the control value is shown in Table 1, and the actual number of thrips captured per trap is changed over time It is shown in the graph of Figure 2.

division processing contents processing time Throughput Prevention (%) Comparative Example 1 No treatment + above-ground pesticides 5/20 - Comparative Example 2 Soil spray + above-ground pesticide 5/20 55 Comparative Example 3 Soil spraying (agrochemical) + drench treatment
+above pesticide 5/20 (drench + pesticide)
6/20 (crossover) 300g/10a+Pesticide
300g/10a 74.6 Example 1 Soil spray + drench treatment
+above pesticide 5/20 (drench + pesticide)
6/20 (crossover) 300g/10a+Pesticide
300g/10a 70.4

As shown in Table 2, in the case of the control method by the microbial preparation according to the method of the present invention of Example 1, it can be confirmed that the control effect is maintained the same in the early stage of crop growth as well as in the middle stage.

Example 2~ 4: processing method control effect ( Early growth (January to April) control effect)

As in Example 1, the control effect was tested by adding additional steps or additives as shown in Table 3 to the control method according to the method of the present invention in which soil spraying and drenching treatment are combined.

The amount of the microbial agent used was the same as in Example 1, and in Table 3, the amount of treatment was indicated only for the additional treatment.

For the control effect, the incidence of thrips was investigated in comparison with the untreated comparative example 1 using the same yellow sticky trap, and the results are shown in Table 3 together.

division processing method When to process Throughput Prevention (%) Comparative Example 1 untreated - - 0 Example 2 formation of moist soil
+Soil spraying + Drench treatment January + January + March Aloe: 1.5kg/10a
Sugar: 750g/10a 85.3 Example 3 Soil spraying (+ sugar)
+ cross-referencing January + March Sugar: 300g/10a 75.3 Example 4 soil spraying+
Drench treatment (+ sugar) January + March Sugar: 300g/10a 78.5 Example 5 Soil spraying (+ aloe complex dry powder irrigation treatment January + March Aloe: 1.5kg/10a 79.3 Example 6 Soil spray + drench treatment (+ sodium bisethylhexylsulfosuccinate) January + March Sodium bisethylhexylsulfosuccinate: 300g/10a 77.8

As shown in Table 3, when spraying with the addition of sugar, aloe complex dry powder, and sodium bisethylhexylsulfosuccinate in the soil spraying step or the drenching treatment step, the effect of increasing the control effect by 5% or more was shown.

In particular, as in Example 2, by pre-forming the moist soil prior to soil spraying, the environment in which the microbial agent can act is improved, and it was found that an improved control effect of about 20% can be obtained.

Claims (3)

Beauveria Basiana bassiana ) KNU-101 strain (accession number KCTC 13660BP), a culture of the strain, a concentrate of the culture, and a microbial agent for killing thrips containing at least one selected from the group consisting of dried products of the culture as an active ingredient In the method for controlling thrips using,
A soil spraying step of spraying the microorganism preparation in an amount of 100 ~ 300g / 10a based on the active ingredient in the soil to plant the crop;
Boveria bassiana KNU-101 strain derived from a drench treatment step of drenching the soil in an amount of 100 to 300 g / 10a based on the active ingredient with the microorganism preparation diluted in water in the state where the crop is planted Thrips control method using microbial preparations of. The method of claim 1, wherein 100 to 500 g / 10a of sugar is mixed with the microbial agent and sprayed together in the soil spraying step or the drenching step. Thrips control method. The method of claim 1, wherein in the irrigation treatment step, sodium bis(2-ethylhexyl) sulfosuccinate or sodium benzoate is added in an amount of 100 to 500 g/10a. A method for controlling thrips using a microbial preparation derived from the Boveria bassiana KNU-101 strain.

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