KR20220169243A - Microorganism immobilized capsule for aquaponics and aquaponics apparatus comprising the same - Google Patents

Abstract

Aquaponics is a system which grows farmed fish or supplies the excrement generated therefrom to crops and uses the same as fertilizer, thereby purifying water quality. However, it is difficult to use pesticides when crops are infected with pests and diseases, and contamination of crops or farmed fish can affect each other. To address these shortcomings, Rhodobacter sphaeroides or Chlorella vulgaris, which are effective for water purification and crop growth, are used in capsules and cultured to purify crops and water quality. As a result of the experiment, Rhodobacter sphaeroides and Chlorella vulgaris were well cultured in the SA-R, C capsules in which the two microorganisms were mixed, ammonia nitrogen in a goldfish excrement solution was reduced, and nitrate nitrogen was decomposed to convert to nitrite nitrogen. As a result of treating cabbage with a goldfish solution mixed with each capsule, when the capsules were processed, the stems and leaves of the cabbage were longer and in good condition compared to the cabbage in the control group. Therefore, it is thought that the efficiency of the system will be improved when SA-R and S, alginate capsules made from R. sphaeroides and C. vulgaris, are applied to the aquaponics.

Description

Microbial immobilization capsule for aquaponics and aquaponics device including the same

The present invention relates to a microbe-immobilized capsule for aquaponics and an aquaponics device including the same, and specifically, aquaponics containing at least one microorganism of Rhodobacter sphaeroides or Chlorella vulgaris It relates to a capsule for immobilizing microorganisms and an aquaponics device including the same.

For the reduction of farmland and the convenience of crop cultivation, a plant factory that grows crops indoors using a plant growth bed and a hydroponic method that can be used in areas with unfavorable geographical conditions because it can grow crops while minimizing soil are used. are doing Recently, the aquaponics system that can grow crops and fish at the same time raises fish on one side and uses the fish excrement as fertilizer for plants, while at the same time the plants purify the water quality and return it to the farmed fish. . This system saves water and fertilizers, allows you to grow crops in the organic farming method favored by consumers, and reduces the amount of soil and waste, which are the advantages of hydroponics.

However, the disadvantages are that it does not completely satisfy the nutrients required for fish, and it is difficult to use pesticides because it can affect fish when crops are plagued by pests, and when crop growth deteriorates, water quality deteriorates together.

Republic of Korea Registration Notice No. 10-2143415

In the present invention, as a method of improving the disadvantages of aquaponics, it was decided to apply microorganisms that can help improve water quality and grow crops and help grow fish. Microalgae, Chlorella vulgaris , and photosynthetic bacteria, Rhodobacter sphaeroides , among microorganisms, are microorganisms that can help water purification, plant growth, and fish farming.

First, Chlorella vulgaris has the effect of removing sludge and heavy metals, and can help aquaculture fish by improving dissolved oxygen in water quality through photosynthesis. In addition, when treated with crops, it contains amino acids and plant growth hormones, so it can help germination and growth of crops such as red pepper. Rhodobacter sphaeroides was observed to decrease COD, total nitrogen, and total phosphorus when treated in farms, and contains amino acids, nucleic acids, and vitamins. When inoculated into cucumbers, the content of Ca, K, and Mg in cucumbers increased. there is

Therefore, the present inventors thought that if the two microorganisms having these advantages were applied to aquaponics, various effects such as crop growth, disease and pest prevention, continuous carbon dioxide reduction, and water quality purification could be obtained. As a method of application, Chlorella vulgaris and Rhodobacter sphaeroides are mixed with each other to make a capsule, and as shown in Fig. 1, the water quality is purified in the first step by placing it in the middle of the rising fish excrement solution, and at the same time, Rhodobacter sphaeroides and Chlorella vulgaris partially come out and crop It was supplied to the plant to see the effect of increasing the growth of crops.

The present invention provides a microbe-immobilized capsule for aquaponics containing at least one microbe selected from Rhodobacter sphaeroides and Chlorella vulgaris .

The microbe-immobilized capsule may be a calcium alginate capsule, but is not limited thereto.

The microbial immobilized capsule may be prepared by mixing a microbial culture medium and a sodium alginate solution and then dropping the mixed solution into a calcium chloride solution, but is not limited thereto.

The sodium alginate solution may be 0.5 to 1% (w/v), but is not limited thereto.

The microbe-immobilized capsule is characterized in that it reduces ammonia nitrogen or nitrate nitrogen and exhibits antibacterial activity.

The microorganism immobilized capsule is characterized in that it is cultured in BG11 medium or NB medium.

According to another embodiment of the present invention, an aquaponics device including the microbe-immobilized capsule is provided.

The aquaponics device may include, but is not limited to, a water tank in which aquatic organisms live, a water filter including the microbe-immobilized capsule, and a plant cultivation unit.

According to another embodiment of the present invention, the step of mixing one or more microorganisms of Rhodobacter sphaeroides or Chlorella vulgaris into a sodium alginate solution; Provided is a method for manufacturing a microbe-immobilized capsule for aquaponics comprising dropping the mixed solution into a calcium chloride solution.

The manufacturing method may further include culturing the microorganism-immobilized capsule in a culture medium.

The medium may be BG11 medium or NB medium, but is not limited thereto.

The microbe-immobilized capsule according to the present invention purifies water contaminated by excretions of aquatic organisms by removing ammonia nitrogen or nitrate nitrogen, and can reduce contamination by exhibiting an antibacterial effect, so that it can be used in aquaponics systems. In this case, it has the effect of increasing the growth rate of crops together with the effect of water purification.

1 is a diagram schematically illustrating the R.sphaeroides and C.vulgaris capsule treatment and water purification process; 1) Fecal transport 2) Primary purified water/crop nutrient supply 3) Secondary purified water supply.
Figure 2 is a photograph showing the appearance of capsules made using C. vulgaris and R. sphaeroides .
Figure 3 is a picture of microorganisms centrifuged after culturing R.sphaeroides and C.vulgaris together and microscopic observation pictures thereof.
Figure 4 is a view showing the results of measuring the growth and chlorophyll absorbance of Chinese cabbage treated with a goldfish excrement solution.
5 is a photograph of SA-R and SA-C capsules after treatment with 0.6% and 0.9% SA-R and SA-C in a goldfish excrement solution for 3 days and a photograph of the capsules observed under a microscope.
Figure 6 is the result of observing the growth length of Chinese cabbage in a solution treated with 0.6% and 0.9% SA-R, SA-C in goldfish excrement solution for 3 days, and Figure 7 is the measurement of absorbance and chlorophyll a / b of the Chinese cabbage This is the graph showing the result.
Figure 8 is a photograph showing the reaction result of nitrate nitrogen in the solution in which the capsule was contained; 1) Appearance of capsules 2) Color change after reagent treatment 3) Nitrate Nitrogen Color Comparison Chart 4) Nitrate Nitrogen Concentration.
Figure 9 is a photograph of observing the nitrate nitrogen color change after each capsule was treated with a goldfish excrement solution.
Figure 10 is a photograph of observing the color change of the capsule after each capsule was treated with a goldfish excrement solution.
11 is a result of measuring ammonia nitrogen after treating a goldfish excrement solution with each capsule; 1) color change 2) ammonia nitrogen color card 3) absorbance change.
12 is a result showing the measurement results of a) nitrate nitrogen and b) nitrite nitrogen after each capsule was treated with a goldfish excrement solution.
13 is a graph showing pH change and COD change after each capsule was treated with a goldfish excreta solution.
14 is a result of observing the growth change of Chinese cabbage in the treated solution after treating the goldfish excrement solution with each capsule.
15 is a) a picture of a cultured fungus after treatment of a goldfish excrement solution with each capsule, and b) a chromatographic picture of a solution extracted from each capsule.

In this specification, when a member is said to be located “on” another member, this includes not only the case where a member is in contact with another member, but also the case where another member exists between the two members.

In this specification, when a part is said to "include" a certain component, it means that it may further include other components without excluding other components unless otherwise stated.

The present invention relates to a microbe-immobilized capsule for aquaponics, and specifically, the capsule may contain at least one microorganism selected from Rhodobacter sphaeroides and Chlorella vulgaris .

The microbe-immobilized capsule is characterized in that it is a calcium alginate capsule, and can be prepared by mixing the microbial culture medium and sodium alginate solution and then dropping the mixed solution into a calcium chloride solution, and then the prepared capsule It can also be cultured in a microbial growth medium.

The microbial culture medium is preferably used for subculture, and the culture medium is a culture medium generally used for culturing microorganisms.

The sodium alginate solution is characterized in that 0.5 ~ 1% (w / v), the sodium alginate solution and the microbial culture is preferably 2: 1 ~ 1: 2 (v / v), the calcium chloride solution is 1 It is preferably ~ 10% (w/v).

The microbe-immobilized capsule is characterized by reducing ammonia nitrogen or nitrate nitrogen. According to the experiment of the present inventors, it was confirmed that ammonia nitrogen was reduced as a result of treating the capsule in a goldfish excretion solution, and furthermore, nitrate nitrogen was decomposed and nitrite nitrogen was increased.

The microorganism immobilized capsule is characterized by exhibiting antibacterial activity, and in aquaponics, when crops are infected with pests, it is difficult to use pesticides and crop growth deteriorates. In the aphonics system, it has the effect of increasing the growth rate of crops and having the ability to continuously purify water quality.

The microorganism immobilized capsule may be cultured in BG11 medium or NB medium, but is not limited thereto.

According to another embodiment of the present invention, an aquaponics device including the microbe-immobilized capsule is provided.

The aquaponics device may include, but is not limited to, a water tank in which aquatic organisms live, a water filter including the microbe-immobilized capsule, and a plant cultivation unit.

1 shows a schematic diagram of an aquaponics device, according to one embodiment of the present invention.

The plant cultivation unit may be disposed above the water tank, but is not limited thereto, and the plant cultivation unit may be composed of a single plant cultivation unit or a plurality of plant cultivation units provided in multiple layers.

The plant cultivation unit is preferably a hydroponic cultivation method.

The plant cultivation unit may include a lighting device for plant cultivation capable of providing light to plants, and the lighting device for plant cultivation is preferably installed above the plant cultivation unit to provide light to plants in the plant cultivation unit.

The water tank is filled with a nutrient solution so that aquatic organisms can inhabit it, and the nutrient solution refers to water that circulates through the tank, a water filter, and a plant cultivation unit to maintain the survival of aquatic organisms and contains nutrients necessary for plant growth. It is the same as the culture medium of meaning.

The water tank, the water filter, and the plant growing unit may be connected through a passage, preferably a hose, to allow the nutrient solution to move, and the aquaponics device may further include a submersible pump capable of circulating the nutrient solution.

The water filter may include the microbe-immobilized capsule of the present invention. The microbe-immobilized capsule may be added at a concentration of 0.01 to 100 g/ml with respect to the nutrient solution, but is not limited thereto.

According to another embodiment of the present invention, the steps of mixing one or more microorganisms of Rhodobacter sphaeroides or Chlorella vulgaris into a sodium alginate solution and dropping the mixed solution into a calcium chloride solution It provides a method for manufacturing a microbiological immobilized capsule for aquaponics comprising.

The manufacturing method may further include culturing the microorganism-immobilized capsule in a medium, but is not limited thereto.

The medium is preferably BG11 medium or NB medium, but is not limited thereto.

The sodium alginate solution is characterized in that 0.5 ~ 1% (w / v), the sodium alginate solution and the microbial culture is preferably 2: 1 ~ 1: 2 (v / v), the calcium chloride solution is 1 It is preferably ~ 10% (w/v).

Hereinafter, the present invention will be described in more detail by examples. These Examples and Experimental Examples are only for explaining the present invention in detail, and it will be apparent to those skilled in the art that the scope of the present invention is not limited to these Examples.

<Experiment method>

One. Rhodobacter sphaeroides Wow Chlorella vulgaris Manufacturing of capsules containing

Sodium alginate (SA) 0.6% 0.9% was put into a 1mL syringe and dropped into a 5% CaCl ₂ solution to make capsules, and Rhodobacter sphaeroides and Chlorella vulgaris were mixed. Thereafter, the capsule made by mixing the two microorganisms was marked as follows (FIG. 2).

i) SA + R.sphaeroides capsules: 0.6% SA-R, 0.9% SA-R

0.6% and 0.9% SA (sodium alginate) and R.sphaeroides culture medium were mixed at a ratio of 1:1 (v/v), and the mixture was put into a syringe and dropped into a 0.5% CaCl ₂ solution to prepare a capsule. It was used after washing with distilled water.

ii) SA + C. vulgaris capsules: 0.6% SA-C. 0.9% SA-C

0.6% and 0.9% SA (sodium alginate) and C.vulgaris culture medium were mixed at a ratio of 1:1 (v/v), and the mixture was put into a syringe and dropped into a 0.5% CaCl ₂ solution to prepare a capsule. It was used after washing with distilled water.

iii) SA + R.sphaeroides + C.vulgaris capsule: 0.9% SA-R,C

0.9% SA, R.spharoides culture medium, and C.vulgaris culture medium were mixed at a ratio of 2:1:1 (v/v), respectively, and the mixture was put into a syringe and dropped into a 0.5% CaCl ₂ solution to prepare a capsule. Washed with distilled water and used.

iv) (SA + R.sphaeroides capsule) + (SA + C.vulgaris capsule): 0.9% SA-R+C

The 0.9% SA-R and 0.9% SA-C capsules prepared in i) and ii) were mixed 1:1 (w/w).

SA-R and SA-C were placed in a petri dish containing goldfish feces and cabbage seeds were placed in it. After one week of growth length and chlorophyll extraction, absorbance was measured at 663 nm and 645 nm. The goldfish excrement solution was used after cutting off the food supply and collecting it from a goldfish tank raised for a week.

In addition, in order to culture the two microorganisms together, goldfish feces were treated with capsules or SA-RC and SA-R+C mixed with the two microorganisms, respectively, and changes in nitrate nitrogen were observed. And based on this result, the experiment was conducted as follows.

2. Water quality change of goldfish excrement solution

The capsules to be placed in the goldfish excreta solution were cultured for 3 days as shown in Table 1 below using BG11 medium in which C. vulgaris grows well and NB medium in which R. sphaeroides grows well, as shown in the table below.

SA-C SA-R,C SA-R,C SA-R SA-R+C culture medium BG11 BG11 NB NB -

The capsules cultured for 3 days in each medium were added to each of 0.4 g in 5mL of the feces solution made by goldfish for 14 days and the feces solution made by goldfish for 21 days. After processing the capsules for 7 days, each solution was collected, and ammonia nitrogen (NH ₄ ⁺ ), nitrate nitrogen (NO ₃ ^- ), and nitrite nitrogen (NO ₂ ^- ) were measured using a water quality measurement kit (Ecosaver). . And the pH of each solution was measured with a pH meter (ISTEC model 735P).

3. Chinese cabbage growth

If each capsule exists, it was confirmed that it could have an effect on crops grown hydroponically by preventing water purification and contamination. I made a groove with a diameter of 1 cm inside the Styrofoam with a thickness of 1 cm and a diameter of 90 mm. After hardening by putting 0.7% Agar into the groove, a total of 14 cabbage seeds were placed, 2 each in each groove. Then, each capsule was placed in a petri dish containing the solution after 9 days of treatment, and the growth of cabbage was observed.

4. Fungal Contamination

While observing the growth of cabbage, mold began to develop on the side where the water quality deteriorated, and it was confirmed how much mold contamination was reduced when each capsule was contained. After 16 days of germination of the cabbage, the Styrofoam put in the Petri dish was taken out, and a portion was collected with tweezers and placed on PDA (Potato Dextrose Agar) to observe the growth of mold.

5. In each capsule Rhodobacter sphaeroides Wow Chlorella vulgaris observation of the growth of

The capsules that had been in the goldfish excreta solution for 9 days were taken out and put in 99% alcohol to extract the pigments of R.sphaeroides and C.vulgaris contained in each capsule. After separation, it was confirmed that R.sphaeroides and C.vulgaris were well cultured in SA-R,C by UV treatment.

<Experiment result>

One. Rhodobacter sphaeroides Wow Chlorella vulgaris growth of

When R.sphaeroides and C.vulgaris were put together in a Petri dish, it was confirmed that they cultured well. The cultured solution for each 3 weeks was centrifuged and observed under a microscope.

Referring to FIG. 3, as a result of centrifugation, small R.sphaeroides with a size of 0.5 to 1 μm was spread widely on the upper side, and C. vulgaris with a relatively large size of 3 to 8 μm was separated on the lower side, and each color was distinctly Observed.

As a result of observation under a microscope, it was confirmed that the cultured side of R. sphaeroides was observed in a pale red color and that the cultured C. vulgaris was in a green color.

2. Growth of Chinese cabbage according to the amount of goldfish excrement solution

Goldfish feces were treated with Chinese cabbage to confirm whether the water actually drawn from the fish could be used as a plant fertilizer.

Referring to FIG. 4, the absorbance of chlorophyll a (663 nm) and chlorophyll b (645 nm) of the cabbage treated with 300 μL of the goldfish excrement solution was higher than that of the control group or the cabbage treated with 100 μL.

Therefore, when C. vulgaris and R. sphaeroides capsules were inserted using goldfish excretory solution, we decided to observe the change in water quality, growth of cabbage, and capsule.

3. Changes in capsules and growth of Chinese cabbage

(1) Changes in capsules according to SA concentration

After treating 0.6%, 0.9% SA-R and 0.6%, 0.9% SA-C in goldfish excretion solution for 3 days, cabbage was treated with each solution and the growth change was observed. First, the change of the capsule according to the SA concentration was observed.

Referring to FIG. 5, R. sphaeroides was darker in color and slightly smaller in size when the concentration of SA was 0.9% than 0.6%. C. vulgaris appeared slightly dark green when the SA concentration was 0.6%, and the size was slightly smaller at 0.9%, similar to R. sphaeroides . As a result of observation under a microscope, it was confirmed that C. vulgaris was well cultured overall and had a green color throughout.

(2) Growth change of Chinese cabbage according to capsule

The growth length of Chinese cabbage treated with the solution containing this capsule was 3 cm and 3.43 cm, respectively, in the control group and SA-only treatment, and in the case of Chinese cabbage treated with SA-C and SA-R solutions, both growth lengths increased (Fig. 6). ).

Absorbance of chlorophyll of Chinese cabbage was measured to be higher in 0.6% SA-C and 0.9% SA-R than in the control group, and the rest were slightly higher or similar to the control group (FIG. 7).

And compared to 3 of chlorophyll a/b in the case of normal plants, 0.9% SA-R was 2.728, which was close to the normal ratio. The growth length, absorbance, and chlorophyll a/b ratio of Chinese cabbage were generally high in the solution containing C. vulgaris and R. sphaeroides .

For this reason, it was thought that C. vulgaris and R. sphaeroides partially escaped from the capsule and helped the growth of Chinese cabbage.

In the reaction of nitrate nitrogen in the solution in which each capsule was contained, the darker the yellow solution, the more nitrate nitrogen was contained. As a result of the experiment, referring to FIG. 8, it was confirmed that all solutions except for the goldfish excrement solution were transparent. Therefore, it was thought that the content of nitrate nitrogen was low because C. vulgaris decomposed and absorbed fecal nitrate nitrogen with nitrogen reductase, and R. sphaeroides also used nitrogen as a nutrient source.

4. Rhodobacter sphaeroides Wow Chlorella vulgaris Water purification of mixed capsules

SA-R,C, which is a capsule mixed with R.sphaeroides and C.vulgaris , and SA-R+C, which are separately made and mixed, are treated with a goldfish excrement solution, and after 3 days, the change in nitrate nitrogen is confirmed to purify water quality to see if it helps.

Referring to Figure 9, it was observed that nitrate nitrogen was reduced than the control group, as if each capsule was treated with a goldfish excrement solution.

Referring to FIG. 10, as a result of culturing each capsule in a petri dish, the color of each capsule gradually darkens, C. vulgaris is dark green, R. sphaeroides is dark red, and SA-R, C is light pink. Incubation was also observed. From these results, it was confirmed that C. vulgaris and R. sphaeroides were cultured well in each capsule, as in the previous result, and nitrate nitrogen was also reduced when cultured.

5. Rhodobacter sphaeroides Wow Chlorella vulgaris Changes in water quality and growth of Chinese cabbage according to capsule treatment

Water was collected on the 14th and 21st days, when the goldfish made feces, respectively, and then the capsules were processed to see if it was helpful in purifying the water quality, and when each capsule was treated, whether it helped the growth of cabbage or not, and whether it could reduce the contamination of crops. confirmed that it exists.

(1) Ammonia nitrogen, nitrate nitrogen, nitrite nitrogen

The concentration of ammonia nitrogen is higher as the color changes to green. In the experiment, most of the light turned green, and the absorbance was measured using a spectrophotometer at 500 m, which is the wavelength at which green is absorbed.

Referring to FIG. 11, as a result of the experiment, the control group was measured to have more ammonia nitrogen on the 21st day than on the 14th day, and this result seems to be due to more goldfish excretion. The decrease in ammonia nitrogen on day 14 was shown in SA-R,C cultured in BG11, SA-R+C mixed with each capsule, and SA-R containing only R.sphaeroides . On day 21, ammonia nitrogen decreased in all but BG11 SA-R,C. In both periods, the decrease in ammonia nitrogen was NB SA-R,C, SA-R+C, and SA-R.

Referring to FIG. 12, it was found that there was little difference in nitrate nitrogen when the collection time was 14 days and 21 days, and when each capsule was inserted.

As a result, the amount of nitrate nitrogen increased as ammonia nitrogen began to decompose over time, and the amount was so large that the degree of decomposition was not observed when each capsule was inserted.

Nitrite nitrogen was pale pink and light orange in both the water collected on the 14th and the water collected on the 21st in the control group, and the color of the BG11 SA-R,C, NB SA-R,C, SA-R+C Considering that the color was relatively dark, it was thought that the three types of capsules decomposed more nitrate nitrogen and increased nitrite nitrogen.

(2) pH change and COD change

Referring to FIG. 13, the pH of the solution collected on the 21st rather than the 14th was gradually lowered overall, and the pH of the solution on the capsule-treated side was higher than that of the control group. The lower pH on day 21 than on day 14 seems to be due to an increase in nitrate nitrogen or nitrite nitrogen as excretion is decomposed. It seems that .sphaeroides used nitrogen salt as a nutrient source.

COD should show a tendency to decrease when R.sphaeroides is included, but COD was measured to be high when R.sphaeroides was included. The cause of the increase in COD is the activation of decomposition of microorganisms or the increase of non-degradable organic matter.

In the experiment, it was considered that the decomposition of microorganisms was activated because there was no non-degradable organic matter. High COD was found in NB SA-R,C and SA-R in which capsules were cultured in NB medium, a condition in which R.sphaeroides grows well, and SA-R+C in which SA-R was mixed with SA-C. Degradation was considered active in this capsule.

(3) Changes in Chinese cabbage growth and mold growth in the capsule-treated solution

The experiment was conducted by making the solution whose water quality change was confirmed above in a form similar to hydroponic culture. Goldfish feces were supplied with solutions collected on the 14th and 21st days.

Referring to FIG. 14, a little fungus was observed around the Chinese cabbage after 7 days, and the growth length and leaves of the control group with a lot of mold propagation were the smallest. Next, the growth of the SA-C capsule was reduced, and the rest of the growth length and leaf length grew normally, and mold growth was reduced. After 10 days, SA-R+C withered the least in the cabbages treated with the solution on day 14, and BG11 SA-R,C, NB SA-R,C, and SA-R among cabbages treated with solution on day 21. It was the least withered. From these results, it appears that crops can tolerate fungal contamination well when R. sphaeroides is contained in capsules, while C. vuglaris is only effective in water purification.

After collecting each Styrofoam with tweezers and incubating it in PDA medium, the Styrofoam treated with the 14-day solution had a wide spread of mold in the order of control, >SA-R>SA-R+C, and the 21-day solution The treated Styrofoam had mold spread in the order of control>SA-R+C>SA-C (FIG. 15a)

SA-R,C cultured in BG11 and NB media showed less mold growth, and when C. vulgaris and R. sphaeroides were included together, fungal contamination was found to be more effective than those made with capsules respectively.

(4) Paper chromatography change of each capsule

When R. sphaeroides and C. vulgaris capsules were mixed with each other or mixed with each other to make capsules and put into goldfish excrement solution, growth was confirmed. After each capsule was extracted with alcohol, developed on paper chromatography and taken to a UV lamp, all of NB SA-R,C, BG11 SA-R,C, SA-R+C, and SA-C containing C.vulgaris A band of chlorophyll responding to ultraviolet light was observed. In addition, red bands were commonly observed in capsules containing R.sphaeroides , and fewer red bands were observed in BG11 SA-R,C, a capsule made by culturing in BG11, and SA-R,C mixed with each capsule (Fig. 15b).

<Conclusion>

Aquaponics has disadvantages in that it is difficult to use pesticides when crops are affected by pests, deterioration of crop growth, and deterioration of water quality. For this reason, it was decided to use C. vulgaris and R. sphaeroides capsules as a way to supplement the disadvantages of aquaponics .

First, both C. vulgaris and R. sphaeroides were cultured well in the capsule (SA-R,C) made by mixing C. vulgaris and R. sphaeroides, and as a result of treating this capsule with a goldfish excrement solution, ammonia nitrogen It was decreased in SA-R,C cultured in NB medium, SA-R+C mixed with each capsule, and SA-C treated only with C. vuglaris than in the control group. Since the concentration of nitrite nitrogen was higher in the treated capsules, it could be expected that nitrate nitrogen was decomposed by R. sphaeroides and C. vulgaris .

When the cabbage was treated with the solution treated with each capsule, the cabbage germinated in the control group, but the growth was inhibited as mold grew around it. However, SA-R, SA-RC, and SA-R were less contaminated with mold and grew better than the control group. When the Styrofoam pieces from each Chinese cabbage were placed in the PDA medium, less mold was also cultured in SA-R,C. Therefore, it was found that contamination can be reduced if R.sphaeroides is included.

Therefore, if SA-R,C is added to the current aquaponics system to supplement it, it is thought that it will be a system that can increase the growth rate of crops and continuously purify water quality.

Claims (12)

A microbe-immobilized capsule for aquaponics comprising one or more microorganisms of Rhodobacter sphaeroides or Chlorella vulgaris .
According to claim 1,
The microorganism immobilized capsule for aquaponics, characterized in that the calcium alginate capsule.
According to claim 1,
The microbial immobilization capsule is prepared by mixing the microbial culture medium and the sodium alginate solution and then dropping the mixed solution into the calcium chloride solution.
According to claim 3,
Microbial immobilization capsules for aquaponics, characterized in that the sodium alginate solution is 0.5 to 1% (w / v).
According to claim 1,
The microorganism immobilization capsule for aquaponics, characterized in that for reducing ammonia nitrogen or nitrate nitrogen.
According to claim 1,
The microorganism immobilized capsule for aquaponics, characterized in that exhibits antibacterial activity.
According to claim 1,
The microorganism immobilized capsule for aquaponics, characterized in that cultured in BG11 medium or NB medium.
An aquaponics device comprising the microbe-immobilized capsule of any one of claims 1 to 7.
According to claim 8,
The aquaponics device comprises a water tank in which aquatic organisms live, a water filter including the microbe-immobilized capsule, and a plant cultivation unit.
Rhodobacter sphaeroides or Chlorella vulgaris Mixing one or more microorganisms of sodium alginate solution;
A method for manufacturing a microbial immobilized capsule for aquaponics comprising the step of dropping the mixed solution into a calcium chloride solution.
According to claim 10,
A method for manufacturing a microbial immobilized capsule for aquaponics, further comprising culturing the microbial immobilized capsule in a medium.
According to claim 11,
The medium is a microbial immobilized capsule manufacturing method for aquaponics, characterized in that BG11 medium or NB medium.

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