KR20220160869A - Method for preparing leaf tea of Gynostemma pentaphyllum and method for preparing Gynostemma pentaphyllum extracts using the leaf tea prepared thereby - Google Patents

Abstract

The present invention relates to a method for preparing Gynostemma pentaphyllum leaf tea containing a high content of low glycoside saponin and reduced benzopyrene and a method for preparing a Gynostemma pentaphyllum leaf tea extract using the Gynostemma pentaphyllum leaf tea prepared thereby. The Gynostemma pentaphyllum leaf tea prepared according to the present invention contains high glycoside saponin in a state of having been already converted into low glycoside so that the Gynostemma pentaphyllum leaf tea itself can be used in various ways, also allows the high content of low-molecular effective saponin to be extracted even by a general method without high-pressure and/or high-temperature treatment and has the low content of benzopyrene, thereby being useful. In addition, the present invention provides the method for preparing the Gynostemma pentaphyllum leaf tea extract, which is useful by obtaining the Gynostemma pentaphyllum leaf tea extract with the high content of low-molecular effective saponin such as ginsenoside Rg3, zipenoside L, zipenoside LI, etc., an enhanced extraction yield rate, and the low content of benzopyrene even without high-pressure and/or high-temperature treatment.

Description

Method for preparing leaf tea of Gynostemma pentaphyllum and method for preparing Gynostemma pentaphyllum extracts using the leaf tea prepared thereby}

The present invention relates to a method for preparing periwinkle leaf tea containing a high content of low glycoside saponin and reduced benzopyrene, and a method for producing an edema leaf tea extract using the edema leaf tea prepared according to the method.

Doloe leaves contain a large amount of saponin glycosides, so they have been widely used as a substitute for ginseng, drinking in the form of tea, or as a raw material for nourishing tonics.

It is well known through previous studies that the saponins, jiphenoside and ginsenosides, contained in ginseng leaf, show the effect of reducing body fat through the activation of AMPK, lowering blood sugar, improving or treating hyperlipidemia, and improving exercise performance. .

By the way, the saponins contained in the leaves are mainly glycosides, and the high glycosides saponins have a high molecular weight and strong polarity, making it difficult to penetrate biological membranes, resulting in low bioavailability. Specifically, when raw leaves are used as they are, bioavailability is very low because the precursors of low-molecular-weight effective saponins present in various ways in the raw leaves are not sufficiently activated. There have been reports of saponins that have not been converted into hypoglycosides converted into hypoglycosidic saponins by intestinal microorganisms. It is emphasized that the process of converting glycoside saponin is necessary.

On the other hand, doloe leaves have been mainly used in the form of doloeip tea prepared by drying after roasting. The roasting process in the process of manufacturing ginguli leaf tea is to separate active ingredients bound to plant tissues from tissues through a high-temperature heat treatment process, and to prevent enzymes present in plant leaves from being activated and decomposing active ingredients after harvesting. . In this regard, Prior Document 1 (Japanese Patent Laid-open Publication No. 1983-170447; processing method of periwinkle) also discloses a method for processing dandelion leaves.

However, since the conventional roasting treatment method is for enzyme inactivation, sterilization of microorganisms, and rapid drying, etc., it is treated for a relatively short time, so there is insufficient time for high glycoside saponin to be sufficiently converted into low molecular weight saponin. In addition, since the main purpose of the roasting process itself is drying, there is a problem in that sufficient desugarization and hydrolysis do not occur due to loss of moisture during the roasting process. Accordingly, there is a limit to the fact that the conventional sedum leaf tea cannot be consumed in a form containing a high content of low-molecular effective saponin.

Meanwhile, in the case of the roasting process, it is well known that benzopyrene may be generated due to high temperature treatment. Since benzopyrene is known as a carcinogen, and 10 ppb or less of benzopyrene is set as an acceptable standard in Europe, a plan to reduce benzopyrene is required in manufacturing tea.

Currently, various methods have been disclosed for increasing the low-molecular effective saponin content of the extract of the ginguli leaf extract, but there is a lack of research on the method for increasing the effective low-molecular-weight saponin content of the leaf tea, which is in greater demand.

Therefore, in the process of manufacturing ginseng leaf tea, the content of dammarin-type low-molecular-weight effective saponins such as ginsenoside Rg3, zipenoside L, zipenoside LI, damulin A, and B, which are major active physiologically active ingredients, is increased while reducing the amount of benzopyrene. A new method of reducing the content is needed.

JP 1983-170447 A KR 10-1986-0000020 A

An object of the present invention is to provide a method for producing ginseng leaf tea in which the high glycoside saponin of ginseng leaf is converted to a low glycoside, and the benzopyrene content is reduced.

Another object of the present invention is to increase the content of effective low-molecular-weight saponins such as ginsenoside Rg3, diphenoside L and diphenoside LI, damulin A and damulin B without high pressure and/or high temperature treatment by using the above-mentioned peony leaf tea. It is an object of the present invention to provide a method for preparing an extract of ginseng leaf tea, in which the extraction yield of low-molecular-weight effective saponin is improved and the content of benzopyrene is low.

In order to achieve the above object, the present invention provides (1) putting dried ginseng leaves in a reaction container and supplying 0.5 to 13 parts by weight of water based on 1 part by weight of the dried ginseng leaves; (2) raising the temperature of the reaction container to 112 to 150 ° C. at a rate of 0.2 to 10 ° C./min to swell the leaves; (3) heating the reaction vessel at 112 to 150° C. for 10 minutes to 24 hours; and (4) releasing the pressure in the reaction vessel to dry the periwinkle leaves.

According to one embodiment of the present invention, the dried periwinkle leaves in the step (1) may be dried after roasting or steaming fresh periwinkle leaves.

According to one embodiment of the present invention, the roasting or steaming may be performed at 90 to 300 ° C for 5 minutes to 120 hours.

According to one embodiment of the present invention, the dried ginkgo biloba leaf in step (1) may have a moisture content of 0.01 to 70% by weight.

According to one embodiment of the present invention, the water content of the outer leaf after step (2) and before step (3) may be 60 to 95% by weight.

According to one embodiment of the present invention, (5) drying the leaf tea; may be further included.

According to one embodiment of the present invention, the ginseng leaf tea contains 0.1 to 2.0 mg/g of ginsenoside Rg3, 0.3 to 8 mg/g of zipenoside L, and 0.3 to 8 mg/g of zipenoside LI based on the weight of the solid content. mg/g, 0.2 to 7 mg/g of damulin A and 0.1 to 6 mg/g of damulin B, and 10 ppb or less of benzopyrene.

In addition, the present invention is prepared according to the above method, 0.1 to 2.0 mg/g of ginsenoside Rg3, 0.3 to 8 mg/g of zipenoside L, and 0.3 to 8 mg of zipenoside LI based on the solid weight. / g, 0.2 to 7 mg/g of damulin A and 0.1 to 6 mg/g of damulin B, and 10 ppb or less of benzopyrene.

In addition, the present invention includes the steps of (1) putting dried rhododendron leaves in a reaction container and supplying 0.5 to 13 parts by weight of water based on 1 part by weight of the dried rhododendron leaves; (2) raising the temperature of the reaction container to 112 to 150 ° C. at a rate of 0.2 to 10 ° C./min to swell the leaves; (3) heating the reaction vessel at 112 to 150° C. for 10 minutes to 24 hours; (4) releasing the pressure of the reaction container to dry the periwinkle leaves; and (5) extracting the dried ginseng leaf tea at 40 to 100 ° C. under atmospheric pressure with hot water or ethanol extraction.

According to one embodiment of the present invention, the ginseng leaf tea extract contains 0.5 to 7.0 mg/g of ginsenoside Rg3, 1.5 to 30 mg/g of zipenoside L, 1.5 to 30 mg/g of zipenoside LI, and damulin A. 2.0 to 20 mg/g and 2.0 to 18 mg/g of damulin B, and benzopyrene may be included at 10 ppb or less.

The leaf tea prepared according to the present invention contains the high glycoside saponin in a state in which it has already been converted into a low glycoside, so that the leaf tea itself can be used in various ways, and can be used in a general way without high pressure and/or high temperature treatment. Even after extraction, a high content of low-molecular-weight effective saponin is extracted, and the content of benzopyrene is low, so it is very useful.

In addition, in the method for preparing an extract of genus japonica leaf tea of the present invention, the extraction yield is also improved while the content of low-molecular effective saponins such as ginsenoside Rg3, zipenoside L, and zipenoside LI is high without high pressure and/or high temperature treatment. It is useful because it is possible to obtain an extract of ginseng leaf tea with a low content of benzopyrene.

1 is a process chart showing a method for manufacturing a peony leaf tea extract according to an embodiment of the present invention.
2 is a graph showing the results of an accelerated stability test of Oesophila leaf tea and Oesophila leaf tea extract according to an embodiment of the present invention.

Hereinafter, the present invention will be described in detail.

Gynostemma pentaphyllum is a perennial vine belonging to the Cucurbitaceae family. It grows naturally in the forests of mountains or fields, and the rhizome extends to the side, has white hairs at the nodes, and grows tangled, but climbs up with tendrils. As a habitat, it grows naturally in the mountains of the southern region, Jeju Island, and Ulleung Island in Korea, and is widely distributed in regions such as China, Japan, and Southeast Asia. Most of them grow in places with high humidity, such as coastal and riversides.

In addition to stones, there are several species depending on the country and region where they grow naturally. Among them, pentaphyllum species are widely distributed, and studies on species, components, and efficacy have been widely conducted. In addition to stones, various saponins are contained, and they are named gynosaponin or gypenoside. When looking at the chemical structure of the diphenoside, only the difference is in which part the OH group is bonded, and the structure has a similar structure to the ginsenoside system of ginseng. Due to the high saponin content of doloe, doloe leaves have been widely drunk as a nutrient tonic substitute for ginseng since ancient times.

Like other teas, the leaves of the leaves have been processed, stored, distributed, and consumed as teas that have gone through a roasting process to prevent deterioration, inactivate the remaining decomposition enzymes, and dry quickly. In recent years, extracts obtained by extracting this doloeip tea and concentrating the saponin content have been used as food and health food.

In the general roasting process, mature fresh leaves are collected, washed in water, placed on a sieve to remove moisture, and dried in a cool, well-ventilated place for 1-2 days to prevent leachate during roasting, or In a state where it is slightly withered by steaming for a short time, put the sedum leaves in a cauldron heated with a high-temperature wood fire (recently, a tumbler with an electric heater can be used) and heat it while stirring continuously. At this time, the moisture inside the outer leaf is rapidly lost, the enzyme is inactivated by the high temperature, and the remaining microorganisms are sterilized. If it is roasted until it is dry at once, the leaves may be burned and leachate may occur, so after the first roasting, they are taken out and rubbed by hand to form a tea shape. Then, by repeating the roasting and rubbing again, a tea shape in which the leaves are clumped together is formed, and when some moisture is removed without leachate, it is common to leave it to cool, cool, and then dry it.

Meanwhile, the roasting process is known to generate benzopyrene. A large amount of benzopyrene is contained in green tea, green tea, red ginseng, coffee, etc., which have been subjected to roasting treatment, and many methods such as steam treatment have been devised to remove it. However, the methods devised for removing the benzopyrene are inconvenient to additionally perform a process other than the grinding process.

The doloe saponin is known to exhibit effects such as lipid metabolism improving action, cardiovascular disease protection action, blood sugar lowering action, central nervous system action, anticancer action, platelet aggregation inhibitory action, and tonic action. In addition, in addition to gypenoside, which is a saponin, doloes contain primeveoside, sophoroside, bisdesmoside, gentiobioside, rutinoside, etc. It contains glycosides, steroids, sugars and pigments.

Most of the saponin compounds contained in the leaves are in the form of glycosides, including sugars, and are high glycoside saponins with low absorption in vivo. Even saponins that are difficult to absorb can sometimes be converted into effective saponins that can be absorbed with low molecular weight. For example, in the case of red ginseng, which contains saponin similar to that of ginseng leaves, there is a report that the bioavailability is increased by converting the high molecular saponin component into low molecular weight (Korean Patent No. 10-1200571). Therefore, conversion of gypenoside glycosides of ginseng leaves into absorbable and effective gifenosides (non-glycosides or low-molecular-weight saponins with small sugar modifications) is a very important factor in increasing bioavailability and thereby increasing effectiveness.

In the present invention, the effective low molecular weight saponin is also referred to as 'low glycoside saponin' and 'low molecular weight saponin'.

The present inventors sufficiently swelled the dry ginseng leaf by supplying water, and sufficiently converted the high glycoside saponin into a low glycoside by heat-treating at a high temperature for a certain period of time in a wet state. In addition, when the swollen ginguli leaves are heat-treated by the method of the present invention, the amount of conversion of proteins, carbohydrates, and lipid components contained in ginseng leaves to benzopyrene is reduced, and Rg3, zipenoside L, zipenoside LI, and The present invention was completed by confirming that the content of low molecular weight effective saponins such as Mullin A and Damulin B increased. In particular, according to the present invention, since benzopyrene formed in the process of roasting, steaming, or swelling the leaves of the genus gyrus and then subjecting them to high-temperature heat treatment is evaporated together with water vapor by releasing the pressure (seal) of the reaction container, benzopyrene content is reduced. can do.

In addition, it was confirmed that the low-molecular effective saponin was sufficiently extracted from the sedum leaf tea prepared in this way even when extracted with general room temperature water or alcohol, and the yield and low-molecular effective saponin content were significantly higher than that of general sedum leaf tea prepared by roasting and drying. In addition, as a result of the accelerated stability test of the leaf tea prepared according to an embodiment of the present invention, it was confirmed that the effective low-molecular saponin was preserved much more stably.

Specifically, the present invention provides a high content of low-molecular effective saponins, such as ginsenoside Rg3, zipenoside L, zipenoside LI, damulin A and damulin B, which are major active physiologically active ingredients, and particularly, a high content of benzopyrene. In order to provide a reduced sedum leaf tea, the present invention includes the steps of (1) putting dried sedum leaf in a reaction container and supplying 0.5 to 13 parts by weight of water with respect to 1 part by weight of the dried sedum leaf; (2) raising the temperature of the reaction container to 112 to 150 ° C. at a rate of 0.2 to 10 ° C./min to swell the leaves; (3) heating the reaction vessel at 112 to 150° C. for 10 minutes to 24 hours; and (4) releasing the pressure in the reaction vessel to dry the periwinkle leaves.

First, in step (1), dry rhizome leaves are put in a reaction container, and 0.5 to 13 parts by weight of water is supplied with respect to 1 part by weight of the dried rhododendron leaves. The reaction vessel may be a pressurized airtight vessel. The present invention is carried out by supplying dried rhododendron leaves and water to the reaction vessel and then sealing it.

The dried eugene leaves mean dried eufolia leaves, and include naturally dried eufolia leaves, semi-dried eufolium leaves, and dried eufolia leaves after roasting or steaming.

Specifically, the dried periwinkle leaves may be dried after roasting or steaming fresh periwinkle leaves at 90 to 300 ° C. for 5 minutes to 120 hours.

Since most of the precursors of the active substances in the outer leaf are glycosides, in principle, when high temperature is applied, the bond with the sugar or lipid components contained in the outer leaf is broken, and it is expected that the content of the active ingredient can be increased. In addition, by applying a high temperature such as roasting, it is possible to inactivate the decomposing enzyme contained in the leaves, thereby suppressing the change of components over time as much as possible.

In general, roasting is a heat treatment of raw leaves at a certain temperature or higher in an open container to sterilize harmful bacteria to prevent deterioration, and to stop decomposition enzyme activity remaining in raw leaves to increase shelf life, aroma and flavor. A process generally performed to be. In roasting, benzopyrene produced due to high temperature or the rate at which active ingredient precursors are converted into active ingredients is not constant due to uneven temperature, not only excessive benzopyrene is produced, but also proceeds in a short time and the conversion rate to low-molecular effective saponin is reduced due to loss of moisture. it gets lower

Therefore, in the present invention, the roasting or steaming is performed at 90 to 300 ° C, preferably 90 to 200 ° C, more preferably 90 to 150 ° C for 20 minutes to 120 hours, preferably 30 minutes to 30 hours, more preferably may be performed for 1 to 10 hours. When roasting or steaming in the above temperature range, it is advantageous to reduce the generation of benzopyrene while preventing the loss of active ingredients contained in the leaves.

In addition, the heat treatment such as roasting or steaming is more preferably a roasting treatment in terms of reducing the benzopyrene content and increasing the active substance content, and the roasting is not particularly limited, but roasting by wood fire, roasting by gas fire, electricity There may be burnt by the heater. The roasting by the wood fire is in a pot heated by a wood fire, the roasting by a gas fire is in a pot heated by a medium gas fire, and the roasting by an electric heater is a rotary electric heater. it could be Among the above methods, roasting by an electric heater is more preferable in order to increase the content of active substances while reducing the content of benzopyrene.

On the other hand, when only natural drying of the leaves without roasting, conversion of high glycoside saponins into effective low molecular weight saponins may hardly be achieved.

The present inventors have found that in order to maximize the content of low molecular weight effective saponin in sedum leaf tea, heat treatment such as roasting should be performed in the raw leaf state. In addition, based on the relationship between saponin precursors and low-molecular-weight effective saponin components (Chen et al., J. Chromatogr. B Analyt Technol Biomed life Sci, 969: pp. 42-52), saponin can It was assumed that the total amount of saponin could be maintained by removing the activity of the degrading enzyme, and that it would be converted into effective low-molecular-weight saponin when subjected to high-temperature heat treatment.

The moisture content of the dried perilla leaf may be 0.01 to 70% by weight, preferably 0.02 to 50% by weight, more preferably 0.03 to 15% by weight.

In the present invention, 0.5 to 13 parts by weight of water, preferably 1 to 10 parts by weight, more preferably 2 to 8 parts by weight, and more preferably 3 to 5 parts by weight, can be supplied based on 1 part by weight of the dried rhododendron leaves. . On the other hand, the amount of water added may be reduced if the non-dried exoskeleton leaves are in a less dry state after being roasted.

As described above, the reason for supplying water to the dried perilla leaves is to sufficiently swell the persimmon leaves by increasing the water content of the persimmon leaves. That is, in the present invention, supplying water to the leaves is only for "swelling" the leaves, and the effectiveness of the leaves is "immersed" or sufficiently supplied with a solvent so that the leaves are completely immersed in the solvent. It is completely different from "extraction", which allows the components to elute into a solvent. When the outer leaf is sufficiently swollen, the conversion rate of the high glycoside saponin to the effective low molecular weight saponin is remarkably increased in the subsequent heat treatment step.

Therefore, in the present invention, the amount of water supplied to the outer leaf is very important. The amount of water to be supplied should be an amount sufficient for the leaves to absorb moisture and "swell" sufficiently. don't

If the amount of water is less than the lower limit, the ginseng leaf may not be sufficiently swollen, and the conversion to low molecular weight effective saponin may not be sufficient in the subsequent heat treatment process. The content of low-molecular-weight effective saponin contained in the leaf tea may rather decrease.

In step (2), the temperature of the reaction vessel is 0.2 to 10 °C/min, preferably 0.2 to 5 °C/min, more preferably 0.2 to 2.5 °C/min, more preferably 0.5 to 2.5 °C/min, still more preferably Preferably, the temperature is raised to 112 to 150 °C, preferably 115 to 130 °C, and more preferably 120 to 130 °C at a rate of 0.5 to 1.5 °C/min. This step is a step for sufficiently swelling the dried perilla leaves.

If the temperature increase rate is less than the lower limit, the time for raising the temperature to the target temperature becomes too long, and if it exceeds the upper limit, the outer leaf may not be sufficiently swollen. In addition, when the temperature is not raised under the above conditions, the dried perilla leaves may not be sufficiently swollen.

Due to the above temperature increase, the outer leaves are sufficiently swollen, and the water content in the outer leaves reaches 60 to 95%, preferably 70 to 90%, and more preferably 75% to 85%. Accordingly, the conversion of the high glycoside saponin into the effective low molecular weight saponin is better achieved in the subsequent heating step.

If the water content in the leaves is less than the lower limit, the swelling of the leaves is not sufficient, so that the conversion of the high glycoside saponin to the low molecular weight effective saponin is not performed better in the subsequent heating step, and if it exceeds the upper limit, It is not economical because the effort to increase the water content in the outer leaf or the cost effect is insignificant.

Next, in step (3), the reaction vessel is heated at 112 to 150 ° C. for 10 minutes to 24 hours. By the heat treatment, in step (2), the low-molecular-weight effective saponin precursors in the leaves swollen by absorbing moisture are deglycosylated and hydrolyzed to be converted into effective low-molecular-weight saponins. In addition, benzopyrene contained in the outer leaf of the stone is evaporated.

The heating temperature may be 112 to 150 °C, preferably 115 to 135 °C, more preferably 120 to 135 °C, and more preferably 120 to 130 °C. When the heating temperature is less than the above lower limit, the conversion rate of the low molecular weight effective saponin precursors into low molecular weight effective saponin decreases and the conversion time becomes longer. When the heating temperature exceeds the upper limit, energy efficiency and safety problems may arise, and decomposition, may cause an increase in benzopyrene.

In addition, the heating time is 10 minutes to 24 hours, preferably 10 minutes to 10 hours, more preferably 20 minutes to 8 hours, more preferably 30 minutes to 8 hours, still more preferably 1 to 4 hours can

When the heating time is less than the above lower limit, the conversion rate of the low molecular weight effective saponin precursors into low molecular weight effective saponin is lowered and benzopyrene evaporation is less likely to occur. , the benzopyrene content will increase.

A specific example of the heat treatment, when the heating temperature is 120 ℃, the heating time may be 1 to 12 hours, preferably 4 to 8 hours, preferably 2 to 4 hours, the heating temperature When the temperature is 130 ° C, the heating time may be 30 minutes to 4 hours, preferably 1 to 3 hours, and when the heating temperature is 140 ° C, the heating time is 20 minutes to 3 hours, preferably 30 minutes to 2 hours. It may be, and when the heating temperature is 150 ℃, the heating time is 10 minutes to 4 hours, preferably 10 minutes to 1 hour, more preferably 20 minutes to 1 hour, more preferably 30 minutes to 1 hour can

Next, in step (4), the pressure of the reaction container is released to dry the periwinkle leaves. Since the reaction vessel is in a state where the temperature and pressure in the reaction vessel are increased due to the heating in step (3), when the pressure in the reaction vessel is released, the water vapor in the reaction vessel rapidly evaporates and the leaves are dried under reduced pressure. Occurs. At the same time, benzopyrene is also evaporated and discharged. Since the specific surface area of sedum leaf tea is much higher than that of sedum leaf tea extract, the benzopyrene removed from the low-temperature vacuum concentration of the extract is insignificant, while the evaporation efficiency of benzopyrene in high-temperature sedum leaf tea is remarkably high.

The outer leaves of Ginseng are in a semi-dry state with a moisture content of less than 80% by weight, preferably 30 to 75% by weight, more preferably 30 to 50% by weight.

The method for producing ginguli leaf tea according to the present invention may be carried out by adding (4-1) additionally drying the dried periwinkle leaves after the step (4).

The drying is not limited in any way, such as hot air drying, freeze drying, vacuum drying, reduced pressure drying, or natural drying.

The drying may be carried out until the moisture content of the leaves is less than 30% by weight, preferably 0.1 to 15% by weight.

Ginsenoside leaf tea of the present invention prepared as described above contains 0.1 to 2.0 mg/g of ginsenoside Rg3, 0.3 to 8 mg/g of zipenoside L, and 0.3 to 8 mg/g of zipenoside LI based on the weight of solid content. g, contains 0.2 to 7 mg/g of damulin A and 0.1 to 6 mg/g of damulin B, and contains 10 ppb or less of benzopyrene.

In addition, ginsenoside Rg3, zipenoside L, zipenoside LI, and other It is very useful because low-molecular effective saponins such as Mulin A and Damulin B are steeped in high content, the extraction yield is increased, and the benzopyrene content is low.

In addition, since the sedum leaf tea prepared according to the present invention is obtained by maximally converting the low-molecular-weight effective saponin precursor in the sedum leaf to the low-molecular-weight saponin, in the case of using the same amount of sedum leaf tea, one or more conditions selected from among solvent, pressure, and temperature It was confirmed through specific experiments that there was no significant difference in the content of the extracted low-molecular-weight effective saponin, even if the extraction was performed differently.

In addition, the present invention includes the steps of (1) putting dried rhododendron leaves in a reaction container and supplying 0.5 to 13 parts by weight of water based on 1 part by weight of the dried rhododendron leaves; (2) raising the temperature of the reaction container to 112 to 150 ° C. at a rate of 0.2 to 10 ° C./min to swell the leaves; (3) heating the reaction vessel at 112 to 150° C. for 10 minutes to 24 hours; (4) releasing the pressure of the reaction container to dry the periwinkle leaves; and (5) extracting the dried ginseng leaf tea at 40 to 100 ° C. under atmospheric pressure with hot water or ethanol extraction.

The method from step (1) to step (4) is as described above.

In step (5), hot water extraction of the leaves recovered in step (4) is carried out under atmospheric pressure at 40 to 100 ° C, preferably 50 to 100 ° C, more preferably 70 to 100 ° C, and still more preferably 80 to 100 ° C. , or ethanol extraction. If the extraction temperature is less than the lower limit, the extraction efficiency of the low molecular weight effective saponin component may decrease, and if it exceeds the upper limit, the extraction efficiency is low compared to the effort and / or cost for increasing the temperature, so it is not economical.

Preferably, the extraction of step (5) is repeated twice, but the first extraction is performed by hot water extraction at 40 to 100 ° C., and the second extraction is performed by 40 to 80% (v / v) ethanol extraction. It is possible to increase the yield and increase the content of the saponin component in the extract of the leaf tea extract. In order to increase the saponin content of the ginseng leaf tea extract, an ethanol aqueous solution may be used as an extraction solvent for both the primary extraction and the secondary extraction, and the number of extractions may exceed two times, but is not preferable in terms of recovery rate and cost.

The content of low-molecular-weight effective saponins such as ginsenoside Rg3, zipenoside L, zipenoside LI, damulin A and damulin B without high-pressure treatment or high-temperature treatment is It is very useful because it has a high extraction yield and a low content of benzopyrene.

In addition, the ginseng leaf tea extract contains 0.5 to 7.0 mg/g of ginsenoside Rg3, 1.5 to 30 mg/g of zipenoside L, 1.5 to 30 mg/g of zipenoside LI, 2.0 to 20 mg/g of damulin A, and Damulin B is included at 2.0 to 18 mg/g, and benzopyrene is included at 10 ppb or less (eg, within the range of 0.01 to 10 ppb).

More specifically, the ginseng leaf tea extract contains ginsenoside Rg3 at 0.5 to 7.0 mg/g, preferably at 0.7 to 5.0 mg/g, more preferably at 0.9 to 4.0 mg/g, and even more preferably at 1.0 to 3.5 mg. You can include it with /g. In addition, 1.5 to 30 mg/g of zipenoside L, preferably 5.0 to 30 mg/g, more preferably 15 to 30 mg/g, and more preferably 20 to 30 mg/g may be included. . In addition, 1.5 to 30 mg/g of zipenoside LI, preferably 5.0 to 20 mg/g, and more preferably 10 to 20 mg/g may be included. In addition, damulin A may be included in an amount of 2.0 to 20 mg/g, preferably 5.0 to 18 mg/g, and more preferably 10 to 16 mg/g. In addition, damulin B may be included in an amount of 2.0 to 18 mg/g, preferably 5.0 to 18 mg/g, and more preferably 8.0 to 16 mg/g.

The extract of the wild leaf tea may be prepared in a concentrated liquid or dry powder state. Specifically, the filtered ginseng leaf tea extract may be used after being concentrated to 60 to 70 brix. Alternatively, the above extract of ginseng leaf tea may be prepared in a powder state that has undergone an additional process such as vacuum drying, freeze drying, or spray drying.

The periwinkle leaf tea or the periwinkle leaf tea extract of the present invention has low benzopyrene content and is less harmful, and has an increased content of low-molecular saponins such as Rg3, zipenoside L, and zipenoside LI, thereby preventing, improving or treating diabetes or obesity. is excellent, and the effect of reducing muscle cell loss and improving exercise performance is excellent.

The present invention also provides a composition comprising the Oedophila leaf tea or the extract of the Oedophila leaf tea of the present invention as an active ingredient. In particular, the composition of the present invention can be used for preventing, improving, or treating obesity, diabetes, or muscle loss, and can be used to improve athletic performance. Depending on the method and purpose of use, the composition of the present invention may be a pharmaceutical composition, food composition, health food composition, quasi-drug composition, etc., but is not limited thereto.

In detail, the pharmaceutical composition of the present invention includes Oesophore leaf tea or an Oedea leaf tea extract as an active ingredient, and may further include a pharmaceutically acceptable carrier. The pharmaceutically acceptable carrier is one commonly used in formulation and includes, but is not limited to, saline solution, sterile water, Ringer's solution, buffered saline solution, cyclodextrin, dextrose solution, maltodextrin solution, glycerol, ethanol, liposome, etc. It is not, and if necessary, other conventional additives such as antioxidants and buffers may be further included. In addition, diluents, dispersants, surfactants, binders, lubricants, etc. may be additionally added to suitably form aqueous solutions, suspensions, fluids, and formulations according to each component. The pharmaceutical composition of the present invention is not particularly limited in formulation, but may be formulated as an injection, oral administration, external skin preparation, and the like.

The pharmaceutical composition may be administered orally or parenterally (for example, intravenously, subcutaneously, intraperitoneally or topically applied) depending on the desired method, and the dosage is the condition and weight of the patient, the severity of the disease, the drug It varies depending on the form, administration route and time, but can be appropriately selected by those skilled in the art.

The dosage level selected from the composition will also depend on the activity of the compound, the route of administration, the severity of the condition being treated and the condition and previous medical history of the patient being treated. However, it is within the knowledge of the art to start with a dose of the compound at a level lower than that required to achieve the desired therapeutic effect and gradually increase the dosage until the desired effect is achieved, and the preferred dosage is age, sex and age. , body type, and weight can be determined. The composition may be further processed, preferably milled or ground into smaller particles, prior to formulation into pharmaceutically acceptable pharmaceutical preparations. The composition will also vary depending on the condition and the patient being treated, but this can be determined non-exclusively. For the desired effect, the effective dose of the Oxygen leaf tea or the extract of the Oxygen leaf tea of the present invention is 0.001 to 400 mg/kg, preferably 0.01 to 100 mg/kg, and may be administered 1 to 3 times a day. The dosage is not intended to limit the scope of the present invention in any way.

The pharmaceutical composition of the present invention is prepared in unit dosage form by formulation using a pharmaceutically acceptable carrier and/or excipient according to a method that can be easily performed by those skilled in the art. or it may be prepared by incorporating into a multi-dose container. At this time, the formulation can be used in any form suitable for pharmaceutical preparations, including oral formulations such as powders, granules, tablets, capsules, suspensions, emulsions, syrups and aerosols, external preparations such as ointments and creams, suppositories and sterile injection solutions, etc. , a dispersing agent or a stabilizing agent may be additionally included.

The present invention also provides a food composition for preventing or improving obesity or diabetes, or muscle loss, comprising the Oesopheles leaf tea or an extract of Oesophageal leaf tea as an active ingredient. The food composition of the present invention includes all forms such as functional food, health functional food, nutritional supplement, health food and food additives. Food compositions of this type can be prepared in various forms according to conventional methods known in the art.

According to the present invention, in the composition, the content of the outer leaf tea or the outer leaf tea extract may be contained in 0.001 to 100% by weight, preferably 0.05 to 50% by weight, based on the total weight of the entire food composition. When the content of the Oedelaxa leaf tea or the Oedelaxa leaf tea extract is less than the above range, there is a problem in that it is difficult to expect the effect of the Oedelaxa leaf tea or the Oedelaxa leaf tea extract.

As a specific example of the food composition, the outer leaf tea or the outer leaf tea extract of the present invention can be prepared in the form of tea, juice or drink for drinking, or granulated, encapsulated, or powdered to be consumed in the form of a health functional food. . In addition, it can be prepared in the form of a composition by mixing the sedum leaf tea or sedum leaf tea extract of the present invention with a known substance or active ingredient known to have an anti-obesity effect, anti-diabetic effect, or muscle loss inhibitory effect. For example, the food composition of the present invention may further contain trace amounts of minerals, vitamins, saccharides, and components having known anti-obesity or anti-diabetic activity, muscle loss inhibitory effects, etc.

In addition, examples of the food composition include drinks, meat, sausages, bread, biscuits, rice cakes, chocolates, candies, snacks, pizza, ramen, other noodles, gums, dairy products including ice creams, various soups, etc. It includes all functional foods in the meaning.

In addition, the health functional food composition is characterized in that it is formulated with one selected from the group consisting of tablets, pills, powders, granules, powders, capsules and liquid formulations, including at least one of carriers, diluents, excipients, and additives .

The Oedlifolia tea or Oedea leaf tea extract of the present invention may be added to food as it is or used together with other foods or food ingredients, and may be appropriately used according to conventional methods. The mixing amount of the active ingredient can be suitably determined depending on the purpose of its use (for prevention or improvement). Generally, the amount of the food composition in the food may be 0.001 to 100% by weight, preferably 0.01 to 50% by weight, more preferably 0.1 to 30% by weight of the total food weight. However, in the case of long-term intake for the purpose of preventing and improving obesity or for health control, the amount may be less than the above range, and since there is no problem in terms of safety, the active ingredient can be used in an amount above the above range. can

Hereinafter, an example will be described in detail with respect to the Oedophila leaf tea or the Oedophila leaf tea extract according to the present invention.

<Example>

Example 1: Manufacture of Ginseng leaf tea - Drying after roasting, supplying moisture (swelling), heat treatment at high temperature

(1) After roasting 10 kg of fresh leaves of Ginkgo biloba using an electric heater at 300 ° C. for 1 hour, they were dried to a moisture content of 8% to obtain 728 g of dried Ginkgo biloba leaves.

Thereafter, 100 g of the dried rhizome leaves and 400 g of water were supplied to a pressurized airtight container and sealed.

(2) Heat was applied to the pressurized airtight container and the temperature was raised to 120 ° C at a rate of 1.0 ° C per minute to swell the outer leaf in the airtight container (moisture content of the outer leaf: 80% by weight).

(3) After that, the pressurized airtight container was heated at 120° C. for 4 hours.

(4) The application of heat to the pressurized airtight container was stopped, and the pressure within the airtight container was released to open it. Then, due to the release of the pressure, the semi-dried leaves were recovered (moisture content of the leaves: 65% by weight).

(5) The recovered Ginkgo biloba leaves were dried with hot air at 65 ° C to obtain 98.5 g of Ginkgo biloba tea with a moisture content of 8%.

(6) 10 g of the above-mentioned peony leaf tea was finely ground with a blender, 100 ml of 70% ethanol was added, extracted three times for 2 hours at 80 ° C., filtered, and dried, and then used as a sample for analyzing the content of peony leaf tea.

Example 2: Manufacture of Ginseng leaf tea extract

1,500 ml of 50% v / v alcohol was added to 100 g of Ginseng leaf tea obtained in step (5) of Example 1, extracted twice for 2 hours at 80 ° C., concentrated under reduced pressure and lyophilized, and then powdered to obtain 31.5 g (extracted Yield 31.5%) was recovered and used as an analysis sample in subsequent experiments.

<Method for analyzing effective small-molecular-weight saponin>

In order to measure the content of active ingredients in the Oesophyte leaf tea according to Example 1 and the extract of Oedesa pedis according to Example 2, HPLC analysis was performed under the conditions and methods shown in Table 1 below. For the analysis, 200 mg of each of the extract samples of the Oesophyte leaf tea according to Example 1 and the extract of the Oedelaxa leaf tea according to Example 2 were taken, dissolved in 5 ml of 50% MeOH, and then used for HPLC analysis. As standards, Damulin A (99.1% purity) and Damulin B (95.96% purity) were from Chengdu Biopurify Phytochemicals (China), Gypenoside L (99.42% purity) was from Shanghai yuanye Bio-Technology (China), Gypenoside LI ( Purity 99.33%) was purchased from Korea Research Institute of Bioscience and Biotechnology, and Gynsenoside Rg3 (purity 98.24%) was purchased from Ambo (Korea). The analysis results are shown in Table 2 below.

Detector DAD or PDA (204 nm) Column ZORBAX Eclipse Plus C18 Flow rate 1.0 mL/min Injection volume 10 µL Mobile phase Solvent A: DW, Solvent B: Acetonitrile Time (min) Solvent A (%) Solvent B (%) 0 60 40 20 60 40 35 50 50 40 50 50 40.1 0 100 50 0 100 50.1 60 40 60 60 40

Classification (mg/g) Gyphenoside L Gyphenoside LI Rg3 closed A closed B Benzopyrene
(ppb) Example 1
(Stone leaf tea) 7.09 4.35 0.81 4.09 3.38 4.5 Example 2
(Golden leaf tea extract) 23.05 13.92 2.65 13.28 11.27 6.8

As shown in Table 2, the Ginseng leaf tea according to Example 1 of the present invention contained 7.09 mg of Gyphenoside L, 4.35 mg of Gifenoside LI, 0.81 mg of Rg3, 4.09 mg of Damulin A, and 3.38 mg of damulin B, and 4.5 ppb of benzopyrene.

In addition, the Ginseng leaf tea extract according to Example 2 of the present invention contained 23.05 mg of zipenoside L, 13.92 mg of zipenoside LI, 2.65 mg of Rg3, 13.28 mg of damulin A, and 11.27 mg of damulin B per 1 g of dry weight. It can be seen that benzopyrene contains 6.8 ppb.

<Test Example>

Test Example 1. Change in active ingredient content according to water supply

In step (1) of Example 2, it was attempted to confirm the change in the active ingredient content of the Oesophore leaf tea extract according to the amount of water supplied.

To this end, by varying the amount of water supplied in step (1) of Example 2 to 0g, 50g, 100g, 200g, 300g, 400g (Example 1), 700g, 1300g, and 2000g, respectively, extract samples were prepared. The content of effective low molecular weight saponin contained in each sample was measured according to the above experimental method, and is shown in Table 3 below. In addition, after step (2) of Example 2 was completed, after collecting the leaves in the airtight container, the water content in the leaves was measured and shown in Table 3 below.

division
(water supply) Gifenoside L (mg/g) Gifenoside LI (mg/g) Rg3
(mg/g) closed A
(mg/g) closed B
(mg/g) Benzopyrene
(ppb) moisture content
(%) 2000g
(1:20) 16.81 10.01 1.52 8.33 6.75 16.8 94.5 1300g
(1:13) 19.31 12.72 1.84 10.46 8.12 9.7 93 700g
(1:7) 20.35 13.12 2.14 12.86 10.42 7.7 87.5 400g
(1:4) 23.05 13.92 2.65 13.28 11.27 6.8 80 300g
(1:3) 22.95 13.94 2.57 13.52 11.32 7.0 75 200g
(1:2) 16.58 9.92 1.51 9.35 6.32 7.5 67 100g
(1:1) 8.27 5.05 0.85 3.53 3.12 11.2 50 50g
(1:0.5) 2.32 1.40 0.25 1.21 1.32 17.5 33 0g
0.00 0.00 0.00 0.00 0.00 28.7 8

Looking at Table 3, it can be seen that no component conversion occurred despite the heat treatment process when water was not supplied to the leaves. When the weight ratio of the leaves and water was 1: 0.5, there was conversion to effective low-molecular saponin, but the conversion rate was low, and it can be confirmed that the conversion to effective low-molecular saponin increases as the amount of water supplied increases. In addition, it can be confirmed that the content of low-molecular-weight effective saponin significantly increased when the weight ratio of the leaves and water was 1:2.

In particular, in the case where the weight ratio of Ginseng leaf and water was 1: 4, as in the example of the present invention, it was confirmed that the low-molecular-weight effective saponin content was remarkably high and the benzopyrene content was the lowest.

On the other hand, when an excessive amount of water was supplied (the weight ratio of periwinkle leaf to water was 1:20), a large amount of leachate was generated and the low molecular weight effective saponin content was lowered. The benzopyrene content was increased.

From the above results, it can be seen that the content of effective low-molecular-weight saponin is significantly increased and the content of benzopyrene is reduced in the leaf tea prepared according to the present invention, compared to the leaf tea prepared without or with excessive water supply.

Test Example 2: Change in active ingredient content according to heating temperature

In order to confirm the change in the active ingredient content according to the heating temperature in step (3) of Example 2, it was contained in the extract prepared by heating at 100 ° C, 110 ° C, 120 ° C, 130 ° C, and 150 ° C for 6 hours, respectively. The content of the active ingredient was measured, and the results are shown in Table 4 below.

Classification (mg/g) Gyphenoside L Gyphenoside LI Rg3 closed A closed B 100℃ 0.11 0.00 0.00 0.01 0.00 110℃ 0.12 0.09 0.00 0.01 0.00 115℃ 6.69 4.73 0.94 4.85 4.52 120℃ 22.98 13.52 2.28 12.95 10.28 130℃ 20.45 13.49 2.83 12.82 10.47 150℃ 17.25 11.31 2.12 11.52 9.34

Looking at Table 4, when the heating temperature was 100 ° C. and 110 ° C., there was no significant change in the content of effective low-molecular-weight saponin contained in the leaves, and it was confirmed that a significant change appeared from 115 ° C. or higher. Therefore, it is assumed that the critical temperature is between 110 and 115 °C. On the other hand, it can be seen that the content of low-molecular-weight effective saponin peaks when heated to 130 ° C, and then gradually decreases as the temperature increases when the temperature exceeds 130 ° C. Since the temperature and the heating time are inversely proportional, increasing the heating temperature requires a shorter heat treatment, but heating for too long is interpreted as a result of decomposition of the active ingredient.

From the above results, it can be seen that the content of low-molecular-weight effective saponin in the leaf tea prepared according to the present invention was significantly increased compared to the leaf tea prepared by heating at 110 ° C or lower. Particularly, it can be seen that the content of low-molecular-weight effective saponin is high when the Ginseng leaf tea prepared according to the present invention is preferably prepared by heating at 120 to 130 °C.

Test Example 3. Change in active ingredient content according to heating time

Prepared by heating at 120 ° C. for 0, 1, 2, 3, 4, 6, 8, 12 and 24 hours, respectively, in order to confirm the change in the active ingredient content according to the heating time in step (3) of Example 2 The content of the active ingredient contained in the extract of the leaf tea was measured, and the results are shown in Table 5 below.

Classification (mg/g) Gyphenoside L Gyphenoside LI Rg3 closed A closed B Benzopyrene
(ppb) 0 hours 0.00 0.00 0.00 0.00 0.00 25.7 1 hours 4.25 3.28 0.52 3.12 2.75 19.3 2 hours 8.45 6.95 1.20 6.23 5.28 12.8 3 hours 20.45 13.49 2.83 12.82 13.47 9.5 4 hours 23.05 13.92 2.65 13.28 11.27 6.8 6 hours 22.98 13.52 2.28 12.95 10.28 7.2 8 hours 20.21 13.28 1.92 12.33 9.82 8.6 12 hours 18.23 11.32 1.01 9.58 7.52 9.8 24 hours 15.35 8.89 0.92 7.52 5.99 11.3

Looking at Table 5, the low-molecular-weight effective saponin content began to increase after 1 hour of heat treatment in step (3), significantly increased after 3 hours, and peaked at 4 to 6 hours. It can be seen that it gradually decreases after 6 hours after taking . The decrease in the content of effective low-molecular-weight saponin with increasing heat treatment time is presumed to be because the effective low-molecular-weight saponin was converted into a more low-molecular-weight form or the saponin was decomposed by long-term high-temperature treatment.

Therefore, for 3 to 12 hours, preferably 3 to 8 hours, more preferably 4 to 6 hours, most preferably 4 It can be confirmed that it was prepared by heating for a period of time.

On the other hand, in the case of benzopyrene, when the heating time was less than 3 hours, the permissible value of 10 ppb was significantly exceeded, but as the heating time increased, it could be confirmed that it was lowered below the permissible value.

Comparative Example 1: Natural drying - general extraction

Dried leaves were prepared by spreading the raw leaves in the shade and naturally drying them to a moisture content of 8%.

The dried Ginseng leaf tea extract was prepared by extracting twice with 80 ° C. alcohol (50% v/v) corresponding to a volume of 15 times, and then concentrated under reduced pressure and lyophilized to powder, and then used as a sample for analysis in the experiment. used

Comparative Example 2: Natural drying - general extraction - heat treatment

The extract of Comparative Example 1 was placed in twice the volume of purified water, sufficiently stirred to suspend, heated at 120 ° C. for 2 hours using a sterilizer, dried and powdered, and then used as an analysis sample in the experiment. .

Comparative Example 3: Natural drying, water supply (swelling), high-temperature heat treatment-general extraction

It was carried out in the same manner as in Example 1, except that the dried persimmon leaves in step (1) were roasted and then naturally dried persimmon leaves in the shade were used instead of dried persimmon leaves.

Comparative Example 4: Drying after roasting - general extraction

After roasting the fresh leaves of dolmens at 150 ° C using an electric heater, they were dried to a moisture content of 8% to prepare dried gilts.

The dried Ginseng leaf tea extract was extracted twice with 80 ° C. alcohol (50% v/v) corresponding to 15 times the volume, and then concentrated under reduced pressure and lyophilized to powder, and then used as a sample for analysis in the experiment. used The average yield of alcohol extraction over the second round was 20.4%.

Comparative Example 5: Drying after grinding treatment, high-temperature heat treatment-general extraction

After roasting the fresh leaves of dolmens at 150 ° C using an electric heater, they were dried to a moisture content of 8% to prepare dried leaves of dolmens.

The dried rhododendron leaves were put in a sterilizer and heated (dry heat treatment) at 120 ° C. for 4 hours.

The heat-treated doloe leaves were extracted twice with 80 ° C. alcohol (50% v / v) corresponding to 15 times the volume to prepare a doloe leaf tea extract, concentrated under reduced pressure and lyophilized to powder, and then analyzed in the experiment was used as

Comparative Example 6: Drying after roasting - high temperature extraction

After roasting the fresh leaves of dolmens at 150 ° C using an electric heater, they were dried to a moisture content of 8% to prepare dried leaves of dolmens.

A 15-fold volume of water was added to the dried leaves, and hot water extraction was performed at 120 ° C. for 2 hours to prepare a hot-water extract. Thereafter, the residue remaining after the hot water extraction was extracted with 80 ° C. alcohol (50% v/v) corresponding to a volume of 15 times to prepare an ethanol extract of periwinkle leaf. The extraction yield was 36.8%. After mixing the hot water extract and the ethanol extract of the rhododendron periwinkle leaf, the mixture was concentrated under reduced pressure and lyophilized to powder, and then used as an analysis sample in the experiment.

Comparative Example 7: Drying after roasting - general extraction - heat treatment

The extract of Comparative Example 4 was placed in twice the volume of purified water, sufficiently stirred to suspend, heated at 120 ° C. for 2 hours using a sterilizer, dried and powdered, and then used as an analysis sample in the experiment. .

Test Example 4. Analysis of Active Ingredient Content of Ginseng Leaf Tea According to Manufacturing Method

Using the analysis samples according to Example 2 and Comparative Example, the content of low-molecular-weight effective saponin contained in each sample was measured in the same manner as in Test Example 1, and is shown in Table 6 below.

Classification (mg/g) Gyphenoside L Gyphenoside LI Rg3 closed A closed B Benzopyrene
(ppb) Example 2 23.05 13.92 2.65 13.28 11.27 6.8 Comparative Example 1 0.00 0.00 0.00 0.00 0.00 1.21 Comparative Example 2 0.00 0.00 0.00 0.00 0.00 1.03 Comparative Example 3 4.48 4.03 1.21 3.00 4.48 2.53 Comparative Example 4 0.17 0.05 0.03 0.00 0.00 25.66 Comparative Example 5 0.39 0.00 0.09 0.00 0.00 15.65 Comparative Example 6 14.02 11.28 1.21 9.82 7.62 12.56 Comparative Example 7 14.44 11.52 1.19 10.53 8.01 16.53

Referring to Table 6, the comparison of the silage leaf tea (or the leaf tea extract using the same) according to the embodiment of the present invention did not go through the temperature increase (swelling) step after supplying water, although dried dol leaf tea was used as a raw material Compared to Examples 4 to 7, it was confirmed that the low molecular weight effective saponin content was significantly higher and the benzopyrene content was significantly lower.

On the other hand, in the case of Comparative Examples 1 to 3 using dried Ginkgo biloba leaves naturally dried without roasting as a raw material, the content of low-molecular-weight effective saponin was below the detection limit or the content was very low.

Test Example 5. Accelerated Stability Test

An accelerated stability test was conducted on each of the Oedlifolia leaf tea powder and the Oedea leaf tea extract powder according to the embodiment of the present invention.

First, after pulverizing the ginseng leaf tea obtained in step (5) of Example 1 to 200 mesh or less, 2 g each was put in an aluminum foil bag and sealed. Each bag containing the sample was stored for 0, 1, 2, 3, 4, 6, 8, and 12 months, respectively, at a temperature of 40 °C and a relative humidity of 70%. Thereafter, each doloeip tea was opened and the content of the active ingredient was analyzed whenever a certain period of time elapsed.

In addition, after storage of the extract powder of Oedeol leaf tea according to Example 2 in the same manner as above, it was opened and the active ingredient content was measured.

The results of the accelerated stability test of the outer leaf tea powder and the outer leaf tea extract powder are shown in FIG. 2 .

Figure 2 shows the total content of 5 types of low-molecular-weight effective saponins (ginsenoside Rg3, zipenoside L, zipenoside LI, damulin A and damulin B) contained in each of the leaf tea powder or the extract powder of the leaf tea extract. is expressed as a relative ratio (%) to the initial value.

Referring to FIG. 2, it was confirmed that both the Oesophila leaf tea powder and the Oesophila leaf tea extract powder according to the embodiment of the present invention maintain the low molecular weight effective saponin content of 80% or more up to 12 months under the accelerated test conditions. In addition, it can be seen that the change in the content of the active ingredient occurs very slowly compared to the extract of the Oedelae leaf tea when stored as the Oedlifolia tea.

<Production Example>

Preparation Example 1: Preparation of tablets

200 mg of the extract powder of the silage leaf tea of Example 1 or the extract powder of the silage leaf tea of Example 2, 9 mg of vitamin E, 9 mg of vitamin C, 200 mg of galactooligosaccharide, 60 mg of lactose, and 140 mg of maltose were mixed and granulated using a fluidized bed dryer. After that, 6 mg of sugar ester is added. Tablets are prepared by compressing 500 mg of these compositions in a conventional manner.

Preparation Example 2: Preparation of capsules

According to the conventional method for preparing soft capsules, 200 mg of the Oesophyte tea powder of Example 1 or the extract powder of the Oedelaxa leaf tea of Example 2, 9 mg of vitamin C, 2 mg of palm oil, 8 mg of hydrogenated vegetable oil, 4 mg of yellow wax, and 9 mg of lecithin were mixed and gelatin capsules were mixed. Filled in to prepare soft capsules.

Preparation Example 3: Preparation of Ginseng leaf tea powder

After finely pulverizing the peony leaf tea of Example 1 using a pin mill, a powder passed through a 300 mesh sieve was obtained.

Preparation Example 4: Preparation of Pills

[0080] The ginguli leaf tea of Example 1 was pulverized to obtain a powder passing through 200 mesh. Pills were prepared after suitably kneading honey, dextrin, starch, microcrystalline cellulose, CMC calcium, etc. with the above-mentioned stone leaf tea powder.

Preparation Example 5: Preparation of concentrated liquid tea

Water was added to the powder of the extract of Pedophilia leaf tea of Example 2 to dilute it into a mixed solution having a solid content of 15%. After heating this at 90 ° C., 10 parts by weight of γ-Cyclodextrin was added to and mixed with respect to 100 parts by weight of the extract powder of Ginseng leaf tea. The mixture was concentrated to 60% to prepare a concentrated liquid tea.

From the above description, those skilled in the art to which the present invention pertains will be able to understand that the present invention can be embodied in other specific forms without changing its technical spirit or essential features. In this regard, the embodiments described above should be understood as illustrative in all respects and not limiting. The scope of the present invention should be construed as including all changes or modifications derived from the meaning and scope of the claims to be described later and equivalent concepts rather than the detailed description above are included in the scope of the present invention.

Claims (11)

(1) putting dried rhododendron leaves in a reaction container and supplying 0.5 to 13 parts by weight of water based on 1 part by weight of the dried rhododendron leaves;
(2) raising the temperature of the reaction container to 112 to 150 ° C. at a rate of 0.2 to 10 ° C./min to swell the leaves;
(3) heating the reaction vessel at 112 to 150° C. for 10 minutes to 24 hours; and
(4) releasing the pressure of the reaction vessel to dry the periwinkle leaves; According to claim 1,
(1) The method of producing doloeip tea, characterized in that the dried doloe leaves are dried after roasting or steaming the doloe leaves. According to claim 2,
The method for producing stone leaf tea, characterized in that the roasting or steaming is performed at 90 to 300 ° C. for 5 minutes to 120 hours. According to claim 1,
(1) The method for producing ginseng leaf tea, characterized in that the dried ginseng leaf tea has a moisture content of 0.01 to 70% by weight. According to claim 1,
The ginseng leaf tea contains 0.1 to 2.0 mg/g of ginsenoside Rg3, 0.3 to 8 mg/g of zipenoside L, 0.3 to 8 mg/g of zipenoside LI, and 0.2 mg/g of damulin A, based on solid weight. ~ 7 mg / g and 0.1 ~ 6 mg / g of damulin B, and benzopyrene of 10 ppb or less. It is prepared according to the method of any one of claims 1 to 4, and contains 0.1 to 2.0 mg/g of ginsenoside Rg3, 0.3 to 8 mg/g of zipenoside L, and zipenoside LI based on solid weight. 0.3 to 8 mg/g, 0.2 to 7 mg/g of damulin A, 0.1 to 6 mg/g of damulin B, and 10 ppb or less of benzopyrene. (1) putting dried rhododendron leaves in a reaction container and supplying 0.5 to 13 parts by weight of water based on 1 part by weight of the dried rhododendron leaves;
(2) raising the temperature of the reaction container to 112 to 150 ° C. at a rate of 0.2 to 10 ° C./min to swell the leaves;
(3) heating the reaction vessel at 112 to 150° C. for 10 minutes to 24 hours;
(4) releasing the pressure of the reaction container to dry the periwinkle leaves; and
(5) extracting the dried ginseng leaf tea at 40 to 100 ° C. under atmospheric pressure with hot water or ethanol extraction; According to claim 7,
(1) A method for producing an extract of doloepiphyllum, characterized in that the dried dolae leaves are dried after roasting or steaming the dolae leaves. According to claim 8,
The roasting or steaming is a method for producing a stone leaf tea extract, characterized in that carried out at 90 to 300 ℃ for 5 minutes to 120 hours. According to claim 7,
A method for producing an extract of periwinkle leaf tea, characterized in that the moisture content of the dried ginseng leaf tea in step (1) is 0.01 to 70% by weight. According to claim 7,
The ginseng leaf tea extract contains 0.5 to 7.0 mg/g of ginsenoside Rg3, 1.5 to 30 mg/g of zipenoside L, 1.5 to 30 mg/g of zipenoside LI, and 2.0 to 20 mg/g of damulin A. g and damulin B in an amount of 2.0 to 18 mg/g, and benzopyrene in an amount of 10 ppb or less.

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