KR20220159875A - Manufacturing method of natural pills - Google Patents

Abstract

The present invention relates to a manufacturing method of natural material pills manufactured by mixing: a fermented liquid produced by aging potato, Capsella bursa-pastoris, sweet persimmon, Allium monanthum, carrot, Codonopsis lanceolata, garlic, radish, water parsley, dandelion, pear, Chinese cabbage, Allium tuberosum, beet, apple, ginger, spinach, Ixeridium dentatum, onion, lotus root, orange, cucumber, burdock, ginseng, and chives; oat powder; barley powder; and honey. The natural material pills manufactured by the method of the present invention have a high flavonoid content with various physiological activities and show excellent immune activity, and thus can provide convenience in intake and use in a pill form while maintaining the nutritional properties of kimchi.

Description

Manufacturing method of natural pills {Manufacturing method of natural pills}

The present invention is a potato, horseradish, sweet persimmon, sweet persimmon, carrot, deodeok, garlic, radish, water parsley, dandelion, pear, Chinese cabbage, leek, beet, apple, ginger, spinach, sageum, onion, lotus root, orange, cucumber, burdock, ginseng , and fermented broth prepared by aging chives; oat flour; barley powder; And it relates to a method for producing a natural product pill prepared by mixing honey.

Kimchi is a traditional Korean lactobacillus fermented food made by fermenting pickled cabbage by salting and draining the water, mixing secondary ingredients and various seasonings. Through the fermentation process, lactic acid bacteria produce organic acids and fermented products using sugars such as cabbage, show taste and health functionality, and produce antibacterial substances such as bacteriocin to suppress the growth of harmful bacteria and give kimchi a unique flavor. grant In addition, kimchi is a nutritionally excellent food with low calorie content and high content of dietary fiber, vitamins and minerals, and contains phytochemicals and phytochemicals such as vitamins, chlorophylls, carotenoids, flavonoids, polyphenols, etc. Various compounds produced by microorganisms during aging are reported to have various functions such as cancer prevention, antioxidant activity, immunity enhancement, intestinal regulation activity, cholesterol lowering, and anti-atherosclerosis. Research is ongoing.

On the other hand, the immune system is a life phenomenon that maintains homeostasis in the body by removing abnormal reactions caused by external factors (antigens). In particular, in modern people, various immune deterioration factors such as various infections, stress, and environmental pollution, confounding factors such as endocrine disruptors, autoimmune diseases such as rheumatism, and increased immune hypersensitivity reactions such as atopy and allergy cause abnormalities in various immune functions. Demand for immunomodulatory foods is increasing to maintain basic homeostasis against various possible factors.

Under this background, the present inventors have made diligent efforts to develop a new processed food that is easy to ingest and carry while maintaining the nutrition and functionality of kimchi containing various natural products. The present invention was completed by confirming that not only was high, but also showed immune activity.

One object of the present invention is potato, horseradish, sweet persimmon, sweet potato, carrot, deodeok, garlic, radish, water parsley, dandelion, pear, Chinese cabbage, leek, beet, apple, ginger, spinach, pickle, onion, lotus root, orange, cucumber , kimchi fermented liquid prepared by aging burdock, ginseng, and chives at 5° C. for 3 months; oat flour; barley powder; And to provide an immunofunctional kimchi pill containing honey.

Another object of the present invention is to provide a method for preparing the kimchi pill.

A detailed description of this is as follows. Meanwhile, each description and embodiment disclosed in the present invention may also be applied to each other description and embodiment. That is, all combinations of the various elements disclosed herein fall within the scope of the present invention. In addition, it cannot be seen that the scope of the present invention is limited by the specific descriptions described below.

One aspect of the present invention for achieving the above object is potato, horseradish, sweet persimmon, sweet potato, carrot, deodeok, garlic, radish, water parsley, dandelion, pear, Chinese cabbage, leek, beet, apple, ginger, spinach, Kimchi fermented liquid prepared by aging onions, lotus roots, oranges, cucumbers, burdock, ginseng, and chives at 5° C. for 3 months; oat flour; barley powder; And it provides an immunofunctional kimchi pill containing honey.

The term "pill" of the present invention means a medicine pill made into a round shape by using purified medicinal materials or natural product powder, honey or grain syrup, and is also called a pill.

The kimchi fermented liquid of the present invention contains 30 parts by weight of potatoes, 50 parts by weight of horseradish, 30 parts by weight of sweet persimmon, 50 parts by weight of wild chives, 20 parts by weight of carrots, 30 parts by weight of deodeok, 20 parts by weight of garlic, 20 parts by weight of radish, and 40 parts by weight of water parsley. , 10 parts by weight of dandelion, 30 parts by weight of pear, 30 parts by weight of Chinese cabbage, 50 parts by weight of chives, 30 parts by weight of beets, 30 parts by weight of apples, 40 parts by weight of ginger, 40 parts by weight of spinach, 30 parts by weight of leeks, 20 parts by weight of onions , 30 parts by weight of lotus root, 30 parts by weight of orange, 20 parts by weight of cucumber, 40 parts by weight of burdock, 40 parts by weight of ginseng, and 40 parts by weight of chives, and was prepared by aging at 5° C. for 3 months.

In a specific embodiment of the present invention, fermented kimchi and kimchi pills containing the same were prepared by varying the content ratio of the 25 natural substances, and the components and activities of each kimchi pill were compared. As a result, the fermented liquid was 30 parts by weight of potatoes, 50 parts by weight of horseradish, 30 parts by weight of sweet persimmon, 50 parts by weight of wild chives, 20 parts by weight of carrots, 30 parts by weight of deodeok, 20 parts by weight of garlic, 20 parts by weight of radish, 40 parts by weight of water parsley, Dandelion 10 parts by weight, pear 30 parts by weight, Chinese cabbage 30 parts by weight, leek 50 parts by weight, beet 30 parts by weight, apple 30 parts by weight, ginger 40 parts by weight, spinach 40 parts by weight, sage 30 parts by weight, onion 20 parts by weight, The kimchi ring of Example 1 containing 30 parts by weight of lotus root, 30 parts by weight of orange, 20 parts by weight of cucumber, 40 parts by weight of burdock, 40 parts by weight of ginseng, and 40 parts by weight of chives has a high phenolic compound content (Experimental Example 1), It was confirmed that the NO generating ability was excellent (Experimental Example 2). That is, the present invention is characterized by identifying the preferred content ratio of each natural substance included in the kimchi fermented broth that exhibits excellent immune activity while maximally extracting functional ingredients.

The potato, horseradish, sweet persimmon, sweet potato, carrot, deodeok, garlic, radish, water parsley, dandelion, pear, Chinese cabbage, leek, beet, apple, ginger, spinach, ssamjang, onion, lotus root, orange, cucumber, burdock, The method of obtaining ginseng and chives is not limited, such as directly collecting them or purchasing and using commercially available products. In addition, the form of using raw material, dry powder, or extract is not limited.

The kimchi pill of the present invention includes 100 parts by weight of fermented kimchi; 40 parts by weight of oat flour; 40 parts by weight of barley powder; and 8 parts by weight of honey. If oat powder or barley powder is included in excess of the above range, molding into a round shape may not be performed. In addition, when honey is included in excess of the above range, it may be difficult to prepare a pill because the dough is sticky.

The fermentation method of the present invention may be a conventional kimchi fermentation method, but is not limited thereto. In a specific embodiment of the present invention, the fermentation was carried out at a temperature of 5 ° C. for 3 months, and the fermentation effect of lactic acid bacteria in the kimchi fermentation broth can be maximized during fermentation by the above method, and accordingly, the physiologically active substances contained in the kimchi fermentation broth Extraction of can proceed preferably.

The kimchi pill of the present invention may further include 20 parts by weight of mushroom powder and 2 parts by weight of honey. The mushroom powder of the present invention includes enoki mushrooms, oyster mushrooms, king oyster mushrooms, and wood ear mushrooms, and more specifically, 30 parts by weight of enoki mushrooms, 30 parts by weight of oyster mushrooms, 10 parts by weight of king oyster mushrooms, and 20 parts by weight of wood ear mushrooms includes wealth The method of obtaining the enoki mushrooms, oyster mushrooms, king oyster mushrooms, and wood ear mushrooms of the present invention is not limited, such as directly collecting them or purchasing and using commercially available products. In addition, the form of using raw material, dry powder, or extract is not limited.

In a specific embodiment of the present invention, it was confirmed that the phenolic compound content of the kimchi ring of Example 2 additionally containing mushroom powder was further increased (Experimental Example 1) and the NO generating ability was also increased (Experimental Example 2).

The term "immunofunctionality" of the present invention means an activity that enhances immune function or helps immune function. Immune function refers to a function of protecting the body from infection, etc., and includes all steps of recognizing foreign substances harmful to the body or abnormally transformed cells and removing them. The immune system is largely divided into innate immunity and acquired immunity, and in the case of innate immunity, it is the primary defense against external infections (antigens) and responds specifically to antigens. Various immune cells such as complement, phagocytes, natural killer cells (NK cells), and dendritic cells are particularly involved in innate immunity. Macrophages, which are representative immune cells related to innate immunity, act as a phagocytic function in tissues and are involved in both innate and acquired immunity. Nitric oxide (NO) is a substance secreted from macrophages activated by stimulants such as LPS, and is known to mediate various immune responses in vivo.

In a specific embodiment of the present invention, the kimchi pills of Example 1 containing the fermented kimchi broth of the present invention and Example 2 further containing mushroom powder were compared to the kimchi pills of Comparative Example containing the fermented kimchi broth of different content ratios. It was confirmed that the polyphenol content and total flavonoid content were high (Experimental Example 1). In addition, as it was confirmed that the NO generating ability was also excellent (Experimental Example 2), it was confirmed that kimchi, which is a daily food, was prepared in a pill form that was easy to carry and ingest, and that the kimchi pill had a function of enhancing in vitro immune activity.

The term "polyphenol" of the present invention is a kind of aromatic alcohol compound found in plants, and is characterized by having a plurality of hydroxyl groups as a functional group having two or more phenol groups in one molecule. Polyphenolic compounds collectively include flavonoids, anthocyanins, tannins, catechins, isoflavones, lignans, and resveratrols, and are widely distributed in the plant kingdom and are contained in large amounts in fruits and leafy vegetables. Several hydroxyl groups (-OH) present in polyphenols have the property of easily combining with various compounds, and are known to have excellent antioxidant, anticancer, and anti-inflammatory effects.

The term "flavonoid" of the present invention is a component belonging to polyphenols, and is a general term for phenolic compounds having C6-C3-C6 as a basic skeleton and exhibiting a yellow or pale yellow color. It is widely distributed in the natural world, and is known to be contained in almost all parts of vegetables and plants, such as leaves, flowers, fruits, stems, and roots, as well as abundantly contained in grains. Flavonoids are known to have high antioxidant capacity by effectively removing reactive oxygen species, and, like polyphenols, antiviral, anticancer, and anti-inflammatory effects have been reported.

The kimchi pill of the present invention is rich in polyphenols and flavonoids showing various physiological activities, so it can be used as a functional food.

Another aspect of the present invention provides a method for producing the immunofunctional kimchi pill.

The description of the "immunofunctionality" and "Kimchi-hwan" is as described above.

The manufacturing method of the immunofunctional kimchi pill of the present invention is a kimchi fermented broth; oat flour; barley powder; and mixing honey.

The kimchi pill of the present invention has a high flavonoid content with various physiological activities and shows excellent immune activity, so it can provide convenience in intake and use as a pill form while maintaining the nutritional properties of kimchi.

1 is a graph comparing the NO production ability of kimchi pills of Examples and Comparative Examples to macrophages.

Hereinafter, the present invention will be described in more detail through examples. However, these examples are intended to illustrate the present invention by way of example, and the scope of the present invention is not limited to these examples.

Preparation Example 1. Preparation of Immunofunctional Kimchi Pills

Preparation Example 1-1. Example 1 Preparation of Kimchi Pills

First, to prepare fermented liquids of 25 vegetables, potatoes, horseradish, sweet persimmon, wild chives, carrots, deodeok, garlic, radish, water parsley, dandelion, pear, Chinese cabbage, leek, beet, apple, ginger, spinach, sage, onion, lotus root , Orange, cucumber, burdock, ginseng, and chives were prepared by washing, drying, and slicing. 150g potato, 250g horseradish, 150g sweet persimmon, 250g wild chive, 100g carrot, 150g deodeok, 100g garlic, 100g radish, 200g water parsley, 50g dandelion, 150g pear, 150g Chinese cabbage, 250g leek, 150g beet, 150g apple, 200g ginger, 200g spinach , 150 g of sagebrush, 100 g of onion, 150 g of lotus root, 150 g of orange, 100 g of cucumber, 200 g of burdock, 200 g of ginseng, and 200 g of chives were put into a casting net and aged at 5 ° C for 3 months. After aging, it was filtered through a sieve to prepare a fermented kimchi broth. Prepared kimchi fermented liquid 100 g of fermented kimchi liquid, 40 g of oat powder, 40 g of barley powder, and 8 g of honey were mixed and molded to complete the kimchi ring of Example 1.

Preparation Example 1-2. Example 2 Preparation of Kimchi Pills

After drying and pulverizing enoki mushrooms, oyster mushrooms, king oyster mushrooms, and wood ear mushrooms to prepare them in powder form, 30 parts by weight of enoki mushrooms, 30 parts by weight of oyster mushrooms, 10 parts by weight of king oyster mushrooms, and 20 parts by weight of wood ear mushrooms were mixed. The mushroom powder of the invention was prepared. Thereafter, the kimchi pill of Example 2 was prepared in the same manner as the kimchi pill of Example 1, but 100 g of fermented kimchi broth, 40 g of oat powder, 40 g of barley powder, 20 g of the mushroom powder, and 10 g of honey were mixed and molded to complete the kimchi pill of Example 2.

Preparation Example 1-3. Preparation of Comparative Examples 1 and 2 Kimchi Pills

It was prepared in the same way as the kimchi pill of Example 1, except that the contents of the components included in the kimchi fermented broth are shown in Table 1 below.

Kimchi fermented liquid ingredients (g) Comparative Example 1 Comparative Example 2 Kimchi fermented liquid ingredients (g) Comparative Example 1 Comparative Example 2 potato 150 150 beat 150 150 Cold 150 200 apologize 150 200 sweet persimmon 150 150 ginger 200 200 soothe 150 150 spinach 200 200 carrot 100 200 Sweep 150 150 Deodeok 150 150 onion 200 100 garlic 100 100 Lotus root 150 150 radish 200 100 Orange 150 200 water parsley 200 200 cucumber 200 100 dandelion 150 50 burdock 200 200 ship 150 200 Ginseng 100 150 napa cabbage 150 150 Chives 200 200 chives 150 200

Specifically, in the case of the kimchi pill of Comparative Example 1, the content of radish, dandelion, onion, and cucumber was increased, and the kimchi pill of Comparative Example 2 was different from the kimchi pill of Example 1 in that the content of carrots, pears, apples, and oranges was increased. there is

Preparation Example 1-4. Comparative Examples 3 and 4 Preparation of kimchi pills

It was prepared in the same way as the kimchi pill of Example 1, except that the contents of the components included in the kimchi fermented broth are shown in Table 2 below.

Kimchi fermented liquid ingredients (g) Comparative Example 3 Comparative Example 4 Kimchi fermented liquid ingredients (g) Comparative Example 3 Comparative Example 4 potato 250 150 beat 250 150 Cold 150 250 apologize 150 150 sweet persimmon 250 150 ginger 100 100 soothe 150 150 spinach 200 200 carrot 100 100 Sweep 150 250 Deodeok 150 250 onion 100 100 garlic 100 200 Lotus root 150 150 radish 100 100 Orange 150 150 water parsley 200 200 cucumber 100 100 dandelion 50 50 burdock 200 200 ship 150 150 Ginseng 200 200 napa cabbage 250 150 Chives 200 200 chives 150 150

Specifically, in the case of the kimchi pill of Comparative Example 3, the contents of potatoes, sweet persimmons, Chinese cabbage, and beets were increased, and the kimchi pill of Comparative Example 4 increased the content of deodeok, garlic, and ssweembagui. The difference from the kimchi pill of Example 1 was have.

Experimental Example 1. Evaluation of the content of physiologically active substances

Flavonoids, which are phenolic compounds, have immunity-enhancing and anti-inflammatory effects, and are widely used as health functional food ingredients because of their strong antioxidant activity. Accordingly, the total phenolic compound and flavonoid contents of the kimchi pill of the present invention were measured. After all of the kimchi pills of Examples and Comparative Examples were homogenized with a homogenizer, 20 times ethanol was added to extract and filter. The recovered filtrates were combined, concentrated, and then centrifuged (7,600 g, 4° C., 30 minutes) to recover only the supernatant to obtain the kimchi pill extract of the present invention.

Then, the content of phenolic compounds in each kimchi ring was compared with Folin-Denis reagent using Folinciocalteu's phenol reagent in each sample, and the absorbance was measured at 760 nm. At this time, the content was calculated from a standard curve obtained by measuring tannic acid at a certain concentration (0 to 500 μg/ml) in the same manner as in the sample. In addition, the total flavonoid content was measured by the method of Moreno et al. 0.1 mL of each extract was mixed with 4.3 mL of 80% ethanol containing 10% aluminum nitrate and 1M potassium acetate, allowed to stand at room temperature for 40 minutes, and then absorbance was measured at 415 nm. At this time, the total flavonoid quantification was calculated from a calibration curve prepared using quercetin to obtain the content.

Total polyphenol content (mg/g) Total flavonoid content (mg/g) Example 1 108.5±0.95 5.4±0.10 Example 2 139.2±0.23 5.1±0.08 Comparative Example 1 56.2±0.29 2.2±0.05 Comparative Example 2 95.6±0.40 4.5±0.01 Comparative Example 3 30.8±0.12 0.7±0.00 Comparative Example 4 65.7±0.03 3.8±0.02

As a result, as shown in Table 3, the total polyphenol content of the kimchi pills of Example 2 was 139.2±0.23 mg/g, which was significantly higher than that of the kimchi pills of Comparative Examples 1 to 4. In addition, in the case of the total flavonoid content, it was confirmed that the Kimchi pill of Example 1 contained the most at 5.4±0.10 mg/g. That is, as a result of preparing kimchi pills by varying the content ratio of each vegetable in the fermented kimchi broth containing 25 vegetables, the content of phenolic compounds having various physiological activities in the kimchi pills of Examples 1 and 2 was the highest. , it was found that the fermented kimchi of the specific formulation of the present invention had the most excellent functionality.

Experimental Example 2. Evaluation of nitric oxide (NO) inducing ability

Nitric oxide (NO) is a substance secreted from macrophages activated by stimulants such as LPS, and is known to mediate various immune responses in vivo. In order to confirm the immune-enhancing effect of the kimchi pill of the present invention, the NO-producing ability of macrophages was evaluated. Specifically, after dispensing Raw264.7 cells into 96-well at 1×10 ⁴ cells/well, the kimchi pill extracts of Examples and Comparative Examples diluted in DMEM medium were treated, and as a positive control, 1 μg/ml of LPS ( Lipopolysaccharide) and cultured for 24 hours. Thereafter, 50 μg of the cell culture supernatant and the same amount of Griess reagent were put in a 96-well plate, reacted at room temperature for 15 minutes, and then measured at 540 nm using a microplate reader. The concentration of nitric oxide (NO) was calculated using a standard curve obtained using sodium nitrite.

As shown in Figure 1, as a result of treating the kimchi ring extract of Examples and Comparative Examples, it was confirmed that the NO production ability increased compared to the control group, although lower than that of the LPS-treated group, which is a positive control group. In particular, it was confirmed that the kimchi pills of Examples 1 and 2 had better NO inducing ability compared to the kimchi pill extract of Comparative Example. That is, since the kimchi pills of Examples 1 and 2 containing the fermented kimchi broth with a specific content ratio of 25 vegetables had high NO secretion activity, it was found that the kimchi pills of the present invention could help improve immunity.

From the above description, those skilled in the art to which the present invention pertains will be able to understand that the present invention may be embodied in other specific forms without changing its technical spirit or essential features. In this regard, it should be understood that the embodiments described above are illustrative in all respects and not limiting. The scope of the present invention should be construed as including all changes or modifications derived from the meaning and scope of the claims to be described later and equivalent concepts rather than the detailed description above are included in the scope of the present invention.

Claims (1)

(a) 150g potato, 250g horseradish, 150g sweet persimmon, 250g wild chive, 100g carrot, 150g deodeok, 100g garlic, 100g radish, 200g water parsley, 50g dandelion, 150g pear, 150g Chinese cabbage, 250g leek, 150g beet, 150g apple, 200g ginger , 200 g of spinach, 150 g of radish, 100 g of onion, 150 g of lotus root, 150 g of orange, 100 g of cucumber, 200 g of burdock, 200 g of ginseng, and 200 g of chives were placed in a cast net, aged at 5 ° C. for 3 months and filtered to prepare a fermentation broth;
(b) preparing mushroom powder by mixing 30 parts by weight of enoki mushrooms, 30 parts by weight of oyster mushrooms, 10 parts by weight of king oyster mushrooms, and 20 parts by weight of wood ear mushrooms; and
(c) mixing and molding 100 g of the fermentation broth, 40 g of oat powder, 40 g of barley powder, 20 g of the mushroom powder, and 8 g of honey;

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