KR20150021675A

KR20150021675A - Method for producing Rhus verniciflua extract with removed toxicity and improved antioxidant activity and Rhus verniciflua extract produced by the same method

Info

Publication number: KR20150021675A
Application number: KR20130098932A
Authority: KR
Inventors: 유창연; 이재근
Original assignee: 강원대학교산학협력단
Priority date: 2013-08-21
Filing date: 2013-08-21
Publication date: 2015-03-03

Abstract

The present invention relates to a method for manufacturing Rhus verniciflua extract with toxicity removed and antioxidant activity improved, a Rhus verniciflua extract with toxicity removed and antioxidant activity improved manufactured by the method, and to a health functional food using the same. Provided in the present invention is a method for removing urushiol of Rhus verniciflua having a trouble of causing lacqure in case of Rhus verniflua extract by an easier method with reduced time and costs thereby industrially using the Rhus verniflua extract having various biological activity, and providing a health functional food safe and beneficial to health of consumers by having excellent antioxidant activity.

Description

TECHNICAL FIELD [0001] The present invention relates to a method for producing an extract of Rhus verniciflua (Rhus verniciflua), which has an antioxidant activity and an antioxidant activity, and a method for producing the extract of Rhus verniciflua (Rhus verniciflua)

The present invention relates to a method for producing an extract of Rhus verniciflua (RVD) having toxicity removed and an antioxidative activity enhanced, a method for removing the toxicity and an antioxidative activity of the extract, and a health functional food using the extract.

Rhus verniciflua ) is introduced from China and is cultivated all over Korea, mainly in Wonju area of Gangwon province. The lacquer tree is used for painting by collecting the sap. The lacquer paint is the best product, and it is widely used because it is well preserved and discolored under any conditions. In one room, lacquer is used as a medicinal material, but its weakness is warm, spicy and toxic, and it is said to have the efficacy of digestion, elimination and sterilization. It is mainly used for erythema (虚血症症), 肺虚 (经 closures), 痛 (心痛) and alluvial (虫产), and the main prescriptions are Gancil Circle, Gunchul San and Lee Sung Hwan.

However, as described above, the lacquer tree, which has many effects on various diseases of the human body, can induce allergic dermatitis and respiratory failure, and a person who has allergies to lacquer can use lacquer There is a problem in that the public can not obtain the beneficial effect of the lacquer tree because the public is afraid of taking the lacquer tree itself.

The poisonous substance that causes the allergy of the Rhus verniciflua is urushiol, which absorbs oxygen in the air by the action of laccase and becomes a black resinous form. The chemical structure of urushiol is a catechol compound having two hydroxyl groups in the benzene ring and having a long chain fatty acid having a carbon number of 15 bonded to the side chain (R). The number of the uriciol derivative Lt; / RTI > Ursiol is the main ingredient in the sap of lacquer trees native to Korea, China and Japan. However, the lacquer tree in Taiwan and Vietnam is mainly composed of laccol, which has 17 branches of catechol in the catechol. In the sap of lacquer tree in Thailand and Myanmar, Unlike urushiol and lacol, thitsiol is known to be the main component, which binds at different positions.

On the other hand, Korean Patent No. 0547554 discloses " a composition containing an extract of sea tangle, garlic and Rhus verniciflua having anti-inflammatory activity ", Korean Patent No. 1159442 discloses a composition comprising sulfuraretin isolated from Rhus verniciflua as an active ingredient A composition for preventing and treating type 1 diabetes mellitus and a health food containing the same is disclosed. However, for the method for preparing the extract of R. lucidum with the toxicity removed and the antioxidative activity enhanced as in the present invention, It has not been revealed.

The present invention has been made in view of the above needs, and it is an object of the present invention to provide an extract of Rhus verniciflua, which is produced by releasing steam generated during hot water extraction at high temperature and high pressure, in water extract of hot water at room temperature and hot water extraction at high temperature and high pressure The results showed that urushiol, which is a toxic substance, was effectively removed from the extract of Rhus verniciflua, which was produced by the release of steam, so that allergen was not induced, . Accordingly, the present invention provides a method of removing urushiol, which is toxic substance of Rhus verniciflua, by reducing the time and expense, and is superior in safety to human body, The present invention provides a method for producing an extract of Rhus verniciflua.

In order to solve the above-mentioned problems, the present invention provides a method for producing an extract of Rhus verniciflua with toxicity removed and antioxidative activity enhanced.

In addition, the present invention provides an extract of Rhus verniciflua with toxicity removed and antioxidant activity enhanced by the above method.

In addition, the present invention provides a health functional food using the extract of Rhus verniciflua with the toxicity removed and the antioxidative activity enhanced.

In the case of the extract of Rhus verniciflua according to the present invention, a method of removing urushiol of Rhus verniciflua, which has a problem of inducing poison ivy, by reducing the time and cost by a more convenient method, And is also excellent in antioxidant effect, so that it can be provided as a health functional food which is safe and beneficial to consumers' health.

FIG. 1 is a graph showing the results of HPLC qualitative analysis for confirming the presence or absence of urushiol in the extract of Rhus verniciflua according to the present invention. FIG. (A): Room temperature extract, (B): Hot high-pressure hot-water extract, (C): Hot-water extract that releases steam generated at high temperature and high pressure.
2 is a graph showing the total phenol content of the Rhus verniciflua extract of the present invention. Con: Extract at room temperature, A: Hot water extract which released steam generated at high temperature and high pressure, B: Hot water high pressure hot water extract.

In order to achieve the object of the present invention,

(a) collected Rhus ( Rhus verniciflua ;

(b) hot water extracting the dried lacquer tree of step (a) at a high pressure; And

and (c) releasing steam generated after the hot water extraction in step (b). The present invention also provides a method for producing an extract of Rhus verniciflua exhibiting enhanced toxicity and enhanced antioxidative activity.

In the production method according to one embodiment of the present invention, the sumac in step (a) is Rhus verniciflua), dog sumac (R. trichocarpa), it may be geomyang sumac (R. succedanea), acid geomyang sumac (R. sylvertris) or sumac (R. javanica), preferably a lacquer tree (Rhus verniciflua . < / RTI >

In the production method according to one embodiment of the present invention, the sumac is not limited to rhizome bark.

In the manufacturing method according to an embodiment of the present invention, in the drying step, the drying may be hot air drying, preferably the lacquer husk is dried at 50 to 80 ° C for 2 to 5 days, more preferably, , But the present invention is not limited thereto.

In the manufacturing method according to an embodiment of the present invention, the high-pressure hot water extraction of step (b) may be performed at a pressure of 0.5 to 1.5 MPa and a temperature of 100 to 120 ° C for 4 to 8 hours, To 1.5 MPa and a temperature of 105 to 115 ° C for 5 to 7 hours, more preferably at a pressure of 1 MPa and at a temperature of 111 ° C for 6 hours, but the present invention is not limited thereto.

More particularly,

(a) collected Rhus ( Rhus verniciflua bark by hot air drying at 50 to 80 ° C for 2 to 5 days;

(b) adding water to the hot-air dried luccus bark of step (a) and subjecting it to hot water extraction at a pressure of 0.5 to 1.5 MPa and a temperature of 100 to 120 ° C for 4 to 8 hours; And

The lacquer tree has various physiological activities, but toxic substance called urushiol (urushiol) causes allergy called lacquer. The Rhus verniciflua extract of the present invention contains a step of immediately releasing steam generated during hot water extraction at the high temperature and high pressure hot water extraction step of the present invention. As a result of the HPLC analysis, the urushiol extract was significantly reduced, (Fig. 1). In addition, as a result of the allergen induction test of the Rhus verniciflua extract of the present invention, no symptom of spots, itching and inflammation occurred at all (Table 3), and the total phenol content was significantly increased and the antioxidant activity by DPPH free radical scavenging Activity analysis showed substantial antioxidant activity (FIG. 2 and Table 4). Therefore, the Rhus verniciflua extract of the present invention can be provided for safe antioxidant use for consumers.

When the extract of the present invention is used as a food additive, it is possible to add the extract of Raccumarum to which toxicity has been removed and the antioxidative activity of which is enhanced, or it can be used together with other food or food ingredients, May be appropriately used depending on the method. The amount of the active ingredient to be mixed can be suitably determined according to its intended use (prevention, health or therapeutic treatment). In general, the extract of Rhus verniciflua, in which the toxicity of the present invention is eliminated and the antioxidative activity is enhanced during the production of food or beverage, is added in an amount of not more than 15 parts by weight, preferably not more than 10 parts by weight, based on the raw material. However, in the case of long-term consumption intended for health and hygiene purposes or for health control purposes, the amount may be less than the above range, and since there is no problem in terms of safety, the active ingredient may be used in an amount exceeding the above range .

There is no particular limitation on the kind of the food. As a specific example, a processed food having improved storage properties can be produced by utilizing the characteristics of agricultural products, livestock products, or aquatic products by using the extract of Rhus verniciflua with the toxicity removed and the antioxidative activity enhanced. Such processed foods include, for example, confectionery, drinks, liquor, fermented foods, canned foods, processed milk products, processed meat products and noodles. The sweets include biscuits, pies, breads, candies, jellies, gums, cereals (including dinner utensils such as cereal flakes) and chocolate. Drinks include carbonated beverages, functional ionic beverages, juices (such as apples, pears, grapes, aloes, citrus fruits, peaches, carrots, tomato juices, etc.) and sikhye. The mainstream includes sake, whiskey, shochu, beer, liquor and fruit wine. Fermented foods include soy sauce, miso, and hot pepper paste. Canned products include canned products (eg, tuna, mackerel, sandwiches, canned fish, etc.), canned products (canned beef, pork, chicken and turkey canned products) and canned products (canned products such as corn, peach and pineapple canned products). Milk processed foods include cheese, butter and yogurt. Meat processed foods include pork cutlet, beef cutlet, chicken cutlet, sausage, sweet and sour pork, nuggets and nubucki. And noodles such as sealed packaging raw noodles. In addition, the composition may be used in retort food, soup and the like.

In addition, functional foods, health foods or health supplements can be prepared using the extract of Rhus verniciflua with the above toxicity removed and the antioxidative activity enhanced. A functional food, a health food or a health supplement food means a food that provides a biocontrol function including a physiologically active ingredient in addition to a nutritional function. The ricin extract having the toxicity of the present invention and the antioxidant activity enhanced, It can be used for the production of functional food, health food or health supplement food because it contains various specific natural products as active ingredients.

Hereinafter, the present invention will be described in detail by way of examples. However, the following examples are illustrative of the present invention, and the present invention is not limited to the following examples.

Experimental Method

1. Plant material and Rhus verniciflua extract

5-year-old Sumac ( Rhus The bark of verniciflua was sampled for 3 days using a hot-air drier at 60 ° C, finely pulverized and placed in a bag (non-woven fabric). The mixture was subjected to room temperature extraction and high- Respectively. The high temperature and high pressure hot water extraction was performed by using a crawling machine (control industry oriental medicine, far infrared ray bio-stove). In the present invention, the Rhus verniciflua extract was used as an effective ingredient in which the naturally chilled extract was extracted after high-temperature high-pressure hot water extraction. In the present invention, the hot water extraction was completed at a pressure of 1 MPa and a temperature of 111 ° C for 6 hours, A step of opening the control valve and forcibly discharging the generated pressure and steam in the high-temperature high-pressure hot water extraction was additionally performed. As a control, to investigate the effect of the room temperature extract extracted at room temperature for 48 hours with steam at room temperature and the effect of steam emission on the change of urushiol content and antioxidant activity, 1000 g of the hot-air dried lacquer bark was used as a control. To ensure no release, completely cooled extracts without valves or lids were used for analysis.

The production conditions of the Rhus verniciflua extract of the present invention division Lacquer bark weight (g) menstruum Air pressure (MPa) Temperature (℃) Extraction time Room temperature extraction 1000 Water (15 l) 0 Room temperature 48 hours High-temperature high-pressure hot water extraction 1000 Water (15 l) One 111 6 hours

2. allergy Triggering experiment

In order to examine the degree of induction of allergic rhizome of rusks extract of Urushichus rhizome extract which released steam generated at high temperature and high pressure according to the present invention, the extract was filtered once a day for 36 days for 12 persons, , And after 36 days, spot, itching and inflammation were confirmed.

3. HPLC Using Urushiol ( urushiol ) Qualitative analysis

Rhus uriciol (15: 1), urushiol (15: 2) and urushiol (15: 3) were obtained from phyto lab Gmbh & Co . &Lt; / RTI > The column used was Atlantls dC18 (3 탆, 2.1 x 150 mm), column oven temperature was 30 캜, flow rate was 0.3 ml / min, and mobile phase was methanol containing 0.1% formic acid and 0.1% formic acid 2, and 10 μl of each of the above extracts at room temperature, high-temperature high-pressure hot-water extract, and hot-water extract from which the high-temperature and high-pressure steam of the present invention was released was injected at a wavelength of 220 nm.

Gradient conditions for HPLC mobile phase for urushiol qualitative analysis Mobile phase Solvent A: Water containing 0.1% formic acid
Solvent B: Methanol containing 0.1% formic acid Gradient conditions

Time (minutes) A (%) B (%) 0 50 50 15 0 100 25 0 100 30 50 50 35 50 50

4. Antioxidant activity

To evaluate the antioxidant activity, the DPPH free radical scavenging method (Zong and Chen, 1996) was measured and modified. Each extract was concentrated and diluted with distilled water at concentrations of 10, 50, 100, 200, 500 and 1000 ppm, respectively. 2 ml of diluted sample per ppm was added to a glass test tube, 2 ml of 0.15 mM DPPH solution was added, and the mixture was allowed to react at room temperature for 30 minutes. After the reaction, the absorbance was measured at a wavelength of 517 nm using a UV spectrophotometer. RC ₅₀ (㎍ / ㎖), which is a 50% reduction in the value of the control group without addition of the compound after the measurement of the absorbance, was shown, and vitamin C (ascorbic acid) used as an antioxidant was used as a positive control.

5. Total phenol content

The total phenolic content was measured using pollin-cytochalochemical reagents that specifically react with phenolic substances and change color (Sato and Ramarathnam, 1996). 50 μl of Pauline-thiocalto reagent was added to 100 μl of sample and stabilized for 5 minutes. Then, 300 μl of 20% Na ₂ CO ₃ was added and stabilized for 15 minutes. Then, 1 ml of distilled water was added to measure the absorbance at 725 nm , And gallic acid were used as calibration curves.

Example 1. Rhus verniciflua extract Urushiol ( urushiol ) Qualitative analysis and allergy Triggering experiment

The poisonous substances that cause allergen in Rhus verniciflua correspond to the peaks detected at retention times of 17, 19, and 21 min with urushiol (15: 1), urushiol (15: 2) and urushiol (15: The results of performing HPLC to analyze the urushiol content of the Rhus verniciflua extract of the present invention are shown in FIG. Uruciol (15: 2) and urushiol (15: 3) were detected similarly at room temperature and high temperature and high pressure hot water extracts, and urushiol (15: 1) was reduced to some extent at high temperature and high pressure hot water extraction. Uruciol (15: 1), urushiol (15: 2) and urushiol (15: 3) were found to be negligible in the case of hot water extracts which released steam generated at high temperature and high pressure.

In order to examine the effect of the extract of Rhus verniciflua on the human body, the extract of the present invention was filtered, and the extracts of 12 rats, which were taken 200 ml once a day for 36 days, The results were as shown in Table 3. No spots and irritations were evident at all, and insignificant levels of itching were observed in 2 patients, but this did not reach the extent of causing spots or inflammation. Thus, it was confirmed that the extract of the present invention did not induce any allergies.

The results of the allergy induction test of the Rhus verniciflua extract of the present invention One 2 3 4 5 6 7 8 9 10 11 12 Half point 0 0 0 0 0 0 0 0 0 0 0 0 itch One One 0 0 0 0 0 0 0 0 0 0 Inflammation 0 0 0 0 0 0 0 0 0 0 0 0

Example 2. Evaluation of Total Phenol Content and Antioxidant Activity of Rhus verniciflua Extract

The calibration curve (R ² = 0.9814) was prepared using gallic acid as a standard substance, and the result of analyzing the total phenol content was as shown in FIG. It was confirmed that the total phenol content was slightly increased in the treatment (52.038 ± 2.04 μg GAE / ㎖) treated with hot water at a high temperature and pressure, compared with the room temperature extract (36.910 ± 1.44 μg GAE / ml) The total phenol content of the extracted hot - water extract was 83.321 ± 0.56 g GAE / ㎖, which was 2.26 times higher than that of the normal temperature extract.

Table 4 shows the results of analysis of DPPH free radical scavenging activity to evaluate the antioxidative activity of the Rhus verniciflua extract of the present invention. The DPPH free radical scavenging ability of the extracts at room temperature was 135.56 ± 0.27 ㎍ / ㎖, but decreased to 109.95 ± 0.18 ㎍ / ㎖ in the hot water extract at high temperature and high pressure. Especially, The hydrothermal extracts which released the resulting steam showed the highest antioxidative activity by decreasing DPPH free radical scavenging ability to 85.95 ± 0.18 μg / ml (Table 4).

The DPPH free radical scavenging ability of Rhus verniciflua extract of the present invention RC ₅₀ ([mu] g / ml) Room temperature extract 135.56 ± 0.27 Hot water extract 109.95 ± 0.18 The hot-water extract of the present invention 85.95 ± 0.18 Vitamin C 5.73 0.30

Claims

(a) collected Rhus ( Rhus verniciflua ;
(b) hot water extracting the dried lacquer tree of step (a) at a high pressure; And
(c) releasing the steam generated after the hot water extraction in the step (b), thereby removing the toxicity and enhancing the antioxidative activity.

The method of claim 1, wherein the lacquer tree in step (a) is lacquer tree bark, wherein the toxicity is removed and the antioxidative activity is enhanced.

The method according to claim 1, wherein the high pressure hot water extraction is hot water extraction at a pressure of 0.5 to 1.5 MPa and a temperature of 100 to 120 ° C for 4 to 8 hours, wherein the toxicity is removed and the antioxidative activity is enhanced.

The method of claim 1, wherein the drying of step (a) is hot air drying.

The method according to claim 1,
(a) collected Rhus ( Rhus verniciflua bark by hot air drying at 50 to 80 ° C for 2 to 5 days;
(b) adding water to the hot-air dried luccus bark of step (a) and subjecting it to hot water extraction at a pressure of 0.5 to 1.5 MPa and a temperature of 100 to 120 ° C for 4 to 8 hours; And
(c) releasing the steam generated after the hot water extraction in the step (b), thereby removing the toxicity and enhancing the antioxidative activity.

An extract of Rhus verniciflua, produced by the method of any one of claims 1 to 5, which is free of toxicity and has enhanced antioxidant activity.

A health functional food using the extract of claim 6, wherein the toxicity is removed and the antioxidant activity is enhanced.