KR20220146601A - 인간 알파-갈락토시다제 변이체 - Google Patents
인간 알파-갈락토시다제 변이체 Download PDFInfo
- Publication number
- KR20220146601A KR20220146601A KR1020227033613A KR20227033613A KR20220146601A KR 20220146601 A KR20220146601 A KR 20220146601A KR 1020227033613 A KR1020227033613 A KR 1020227033613A KR 20227033613 A KR20227033613 A KR 20227033613A KR 20220146601 A KR20220146601 A KR 20220146601A
- Authority
- KR
- South Korea
- Prior art keywords
- galactosidase
- sequence
- alpha
- seq
- recombinant
- Prior art date
Links
- 108010030291 alpha-Galactosidase Proteins 0.000 title claims abstract description 49
- 102000005840 alpha-Galactosidase Human genes 0.000 title claims abstract description 48
- 241000282414 Homo sapiens Species 0.000 title claims abstract description 44
- 108090000765 processed proteins & peptides Proteins 0.000 claims abstract description 370
- 229920001184 polypeptide Polymers 0.000 claims abstract description 364
- 102000004196 processed proteins & peptides Human genes 0.000 claims abstract description 364
- 239000000203 mixture Substances 0.000 claims abstract description 49
- 210000002966 serum Anatomy 0.000 claims abstract description 16
- 230000002829 reductive effect Effects 0.000 claims abstract description 9
- 230000005847 immunogenicity Effects 0.000 claims abstract description 8
- 108010049936 agalsidase alfa Proteins 0.000 claims description 208
- 101000718525 Homo sapiens Alpha-galactosidase A Proteins 0.000 claims description 206
- 238000006467 substitution reaction Methods 0.000 claims description 177
- 102000040430 polynucleotide Human genes 0.000 claims description 147
- 108091033319 polynucleotide Proteins 0.000 claims description 147
- 239000002157 polynucleotide Substances 0.000 claims description 146
- 210000004027 cell Anatomy 0.000 claims description 137
- 125000003275 alpha amino acid group Chemical group 0.000 claims description 114
- 239000012634 fragment Substances 0.000 claims description 97
- 238000000034 method Methods 0.000 claims description 62
- 239000013604 expression vector Substances 0.000 claims description 29
- 230000001105 regulatory effect Effects 0.000 claims description 27
- 208000024720 Fabry Disease Diseases 0.000 claims description 24
- 239000008194 pharmaceutical composition Substances 0.000 claims description 19
- 238000011282 treatment Methods 0.000 claims description 19
- 108020004414 DNA Proteins 0.000 claims description 17
- 108020004999 messenger RNA Proteins 0.000 claims description 17
- 238000004519 manufacturing process Methods 0.000 claims description 13
- 102000043404 human GLA Human genes 0.000 claims description 11
- 208000024891 symptom Diseases 0.000 claims description 11
- 230000035772 mutation Effects 0.000 claims description 10
- 210000003712 lysosome Anatomy 0.000 claims description 8
- 230000001868 lysosomic effect Effects 0.000 claims description 8
- 210000004962 mammalian cell Anatomy 0.000 claims description 8
- 239000000546 pharmaceutical excipient Substances 0.000 claims description 7
- 235000005911 diet Nutrition 0.000 claims description 6
- 238000012258 culturing Methods 0.000 claims description 5
- 210000003527 eukaryotic cell Anatomy 0.000 claims description 5
- 230000001747 exhibiting effect Effects 0.000 claims description 5
- 238000002347 injection Methods 0.000 claims description 5
- 239000007924 injection Substances 0.000 claims description 5
- 230000003197 catalytic effect Effects 0.000 claims description 4
- 230000037213 diet Effects 0.000 claims description 4
- 238000001802 infusion Methods 0.000 claims description 4
- 239000003937 drug carrier Substances 0.000 claims description 3
- 210000001236 prokaryotic cell Anatomy 0.000 claims description 3
- 101000718529 Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) Alpha-galactosidase Proteins 0.000 claims 2
- NNISLDGFPWIBDF-MPRBLYSKSA-N alpha-D-Gal-(1->3)-beta-D-Gal-(1->4)-D-GlcNAc Chemical compound O[C@@H]1[C@@H](NC(=O)C)C(O)O[C@H](CO)[C@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](O)[C@@H](O)[C@@H](CO)O2)O)[C@@H](O)[C@@H](CO)O1 NNISLDGFPWIBDF-MPRBLYSKSA-N 0.000 claims 2
- 108091032973 (ribonucleotides)n+m Proteins 0.000 claims 1
- 230000002378 acidificating effect Effects 0.000 abstract description 15
- 230000004700 cellular uptake Effects 0.000 abstract description 14
- 230000001225 therapeutic effect Effects 0.000 abstract description 6
- 101000589519 Homo sapiens N-acetyltransferase 8 Proteins 0.000 description 195
- 102100026277 Alpha-galactosidase A Human genes 0.000 description 187
- 235000020664 gamma-linolenic acid Nutrition 0.000 description 186
- 102220282124 rs1375178645 Human genes 0.000 description 145
- 235000001014 amino acid Nutrition 0.000 description 144
- 102200096040 rs34030778 Human genes 0.000 description 141
- 102220414643 c.28C>A Human genes 0.000 description 120
- 102220367041 c.140C>G Human genes 0.000 description 96
- 102220619547 L-lactate dehydrogenase B chain_T10P_mutation Human genes 0.000 description 84
- 102200123127 rs1555621454 Human genes 0.000 description 75
- 229940024606 amino acid Drugs 0.000 description 61
- 102220468801 Peptidyl-tRNA hydrolase ICT1, mitochondrial_L44R_mutation Human genes 0.000 description 57
- 150000001413 amino acids Chemical class 0.000 description 57
- 230000000694 effects Effects 0.000 description 56
- 102220568068 Tetratricopeptide repeat protein 4_S47T_mutation Human genes 0.000 description 53
- 108090000623 proteins and genes Proteins 0.000 description 53
- 102220486126 Alpha-galactosidase A_E48D_mutation Human genes 0.000 description 51
- 102000004190 Enzymes Human genes 0.000 description 43
- 108090000790 Enzymes Proteins 0.000 description 43
- 229940088598 enzyme Drugs 0.000 description 43
- 230000014509 gene expression Effects 0.000 description 33
- 150000007523 nucleic acids Chemical class 0.000 description 30
- 239000013598 vector Substances 0.000 description 27
- 102220635299 Adenylate kinase isoenzyme 6_R44V_mutation Human genes 0.000 description 24
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 24
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 24
- 238000006243 chemical reaction Methods 0.000 description 23
- 235000018102 proteins Nutrition 0.000 description 23
- 102000004169 proteins and genes Human genes 0.000 description 23
- 108010076504 Protein Sorting Signals Proteins 0.000 description 22
- 102220509487 Kinesin-like protein KIF2C_S95E_mutation Human genes 0.000 description 21
- 102220497176 Small vasohibin-binding protein_T47D_mutation Human genes 0.000 description 21
- 108091026890 Coding region Proteins 0.000 description 20
- 238000003556 assay Methods 0.000 description 20
- 125000000539 amino acid group Chemical group 0.000 description 19
- 108020004705 Codon Proteins 0.000 description 18
- 102200076842 rs869312134 Human genes 0.000 description 18
- 102000039446 nucleic acids Human genes 0.000 description 17
- 108020004707 nucleic acids Proteins 0.000 description 17
- 102000053602 DNA Human genes 0.000 description 16
- 108091028043 Nucleic acid sequence Proteins 0.000 description 16
- 239000000872 buffer Substances 0.000 description 16
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 16
- 230000007062 hydrolysis Effects 0.000 description 16
- 238000006460 hydrolysis reaction Methods 0.000 description 16
- 239000000047 product Substances 0.000 description 16
- 102220524532 Tumor necrosis factor receptor superfamily member 19_S31T_mutation Human genes 0.000 description 15
- 230000002255 enzymatic effect Effects 0.000 description 15
- 102220125096 rs886044878 Human genes 0.000 description 15
- -1 316M Inorganic materials 0.000 description 14
- YUDPTGPSBJVHCN-CHUNWDLHSA-N 4-methylumbelliferyl alpha-D-galactoside Chemical compound C1=CC=2C(C)=CC(=O)OC=2C=C1O[C@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O YUDPTGPSBJVHCN-CHUNWDLHSA-N 0.000 description 14
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 14
- 238000012217 deletion Methods 0.000 description 14
- 230000037430 deletion Effects 0.000 description 14
- 201000010099 disease Diseases 0.000 description 14
- 210000001519 tissue Anatomy 0.000 description 14
- 238000009396 hybridization Methods 0.000 description 13
- 241000894007 species Species 0.000 description 13
- 102220585330 Serine/threonine-protein kinase STK11_R87K_mutation Human genes 0.000 description 12
- 230000000875 corresponding effect Effects 0.000 description 12
- 102220231667 rs150656720 Human genes 0.000 description 12
- 102200088010 rs63750664 Human genes 0.000 description 12
- 239000006228 supernatant Substances 0.000 description 12
- 239000000126 substance Substances 0.000 description 11
- 239000000758 substrate Substances 0.000 description 11
- 238000001415 gene therapy Methods 0.000 description 10
- 102220582725 Heparan sulfate glucosamine 3-O-sulfotransferase 2_H92A_mutation Human genes 0.000 description 9
- 102220628675 NPC intracellular cholesterol transporter 1_Q88A_mutation Human genes 0.000 description 9
- 238000003780 insertion Methods 0.000 description 9
- 230000037431 insertion Effects 0.000 description 9
- 230000002132 lysosomal effect Effects 0.000 description 9
- 238000002703 mutagenesis Methods 0.000 description 9
- 231100000350 mutagenesis Toxicity 0.000 description 9
- 102200020106 rs1131691977 Human genes 0.000 description 9
- 102220287941 rs1555932714 Human genes 0.000 description 9
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 description 8
- 241001465754 Metazoa Species 0.000 description 8
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 8
- 150000001875 compounds Chemical class 0.000 description 8
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 8
- 230000005284 excitation Effects 0.000 description 8
- 238000009472 formulation Methods 0.000 description 8
- 230000006872 improvement Effects 0.000 description 8
- 239000002953 phosphate buffered saline Substances 0.000 description 8
- 239000013612 plasmid Substances 0.000 description 8
- 230000008488 polyadenylation Effects 0.000 description 8
- 241000701022 Cytomegalovirus Species 0.000 description 7
- AFYNADDZULBEJA-UHFFFAOYSA-N bicinchoninic acid Chemical compound C1=CC=CC2=NC(C=3C=C(C4=CC=CC=C4N=3)C(=O)O)=CC(C(O)=O)=C21 AFYNADDZULBEJA-UHFFFAOYSA-N 0.000 description 7
- 230000015556 catabolic process Effects 0.000 description 7
- 239000003153 chemical reaction reagent Substances 0.000 description 7
- 230000002538 fungal effect Effects 0.000 description 7
- 238000011534 incubation Methods 0.000 description 7
- 239000002502 liposome Substances 0.000 description 7
- 229910000029 sodium carbonate Inorganic materials 0.000 description 7
- 238000013518 transcription Methods 0.000 description 7
- 230000035897 transcription Effects 0.000 description 7
- 230000014616 translation Effects 0.000 description 7
- 239000004382 Amylase Substances 0.000 description 6
- 108010065511 Amylases Proteins 0.000 description 6
- 102000013142 Amylases Human genes 0.000 description 6
- 241000228212 Aspergillus Species 0.000 description 6
- 241000228245 Aspergillus niger Species 0.000 description 6
- 102220486708 Cytochrome b-245 chaperone 1_R44G_mutation Human genes 0.000 description 6
- 244000207740 Lemna minor Species 0.000 description 6
- 235000006439 Lemna minor Nutrition 0.000 description 6
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 6
- 235000001855 Portulaca oleracea Nutrition 0.000 description 6
- 102220577769 Respirasome Complex Assembly Factor 1_H92D_mutation Human genes 0.000 description 6
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 6
- 239000002253 acid Substances 0.000 description 6
- 235000019418 amylase Nutrition 0.000 description 6
- 238000004458 analytical method Methods 0.000 description 6
- 210000004369 blood Anatomy 0.000 description 6
- 239000008280 blood Substances 0.000 description 6
- 210000002950 fibroblast Anatomy 0.000 description 6
- 239000000499 gel Substances 0.000 description 6
- 230000007935 neutral effect Effects 0.000 description 6
- 239000002773 nucleotide Substances 0.000 description 6
- 125000003729 nucleotide group Chemical group 0.000 description 6
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 6
- 238000000746 purification Methods 0.000 description 6
- 238000011160 research Methods 0.000 description 6
- 102220326975 rs770967343 Human genes 0.000 description 6
- 238000013519 translation Methods 0.000 description 6
- 241000701161 unidentified adenovirus Species 0.000 description 6
- 102000012288 Phosphopyruvate Hydratase Human genes 0.000 description 5
- 108010022181 Phosphopyruvate Hydratase Proteins 0.000 description 5
- 241000700605 Viruses Species 0.000 description 5
- 230000001186 cumulative effect Effects 0.000 description 5
- 230000003247 decreasing effect Effects 0.000 description 5
- 238000006731 degradation reaction Methods 0.000 description 5
- 239000003814 drug Substances 0.000 description 5
- 239000001963 growth medium Substances 0.000 description 5
- 238000004128 high performance liquid chromatography Methods 0.000 description 5
- 239000006166 lysate Substances 0.000 description 5
- 238000003259 recombinant expression Methods 0.000 description 5
- 229920002477 rna polymer Polymers 0.000 description 5
- 239000000243 solution Substances 0.000 description 5
- 108020005345 3' Untranslated Regions Proteins 0.000 description 4
- 239000004475 Arginine Substances 0.000 description 4
- 241000351920 Aspergillus nidulans Species 0.000 description 4
- 101000757144 Aspergillus niger Glucoamylase Proteins 0.000 description 4
- 108010062580 Concanavalin A Proteins 0.000 description 4
- ZHNUHDYFZUAESO-UHFFFAOYSA-N Formamide Chemical compound NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 description 4
- 108700007698 Genetic Terminator Regions Proteins 0.000 description 4
- QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 description 4
- 102000035195 Peptidases Human genes 0.000 description 4
- 108091005804 Peptidases Proteins 0.000 description 4
- 239000004365 Protease Substances 0.000 description 4
- 208000037065 Subacute sclerosing leukoencephalitis Diseases 0.000 description 4
- 206010042297 Subacute sclerosing panencephalitis Diseases 0.000 description 4
- 238000001042 affinity chromatography Methods 0.000 description 4
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 4
- 229910002091 carbon monoxide Inorganic materials 0.000 description 4
- 239000003636 conditioned culture medium Substances 0.000 description 4
- 230000002068 genetic effect Effects 0.000 description 4
- 102000006602 glyceraldehyde-3-phosphate dehydrogenase Human genes 0.000 description 4
- 108020004445 glyceraldehyde-3-phosphate dehydrogenase Proteins 0.000 description 4
- 210000002216 heart Anatomy 0.000 description 4
- 239000004615 ingredient Substances 0.000 description 4
- 239000011159 matrix material Substances 0.000 description 4
- 230000004048 modification Effects 0.000 description 4
- 238000012986 modification Methods 0.000 description 4
- 229920000642 polymer Polymers 0.000 description 4
- 230000004481 post-translational protein modification Effects 0.000 description 4
- 239000011780 sodium chloride Substances 0.000 description 4
- 239000007787 solid Substances 0.000 description 4
- 230000002103 transcriptional effect Effects 0.000 description 4
- 238000001890 transfection Methods 0.000 description 4
- 238000005406 washing Methods 0.000 description 4
- 108020003589 5' Untranslated Regions Proteins 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 3
- 102220518628 Baculoviral IAP repeat-containing protein 6_T47E_mutation Human genes 0.000 description 3
- 102220477626 Bis(5'-nucleosyl)-tetraphosphatase [asymmetrical]_K36M_mutation Human genes 0.000 description 3
- 102220616204 CCR4-NOT transcription complex subunit 4_R44E_mutation Human genes 0.000 description 3
- 102220596411 Centrosomal protein of 63 kDa_H92L_mutation Human genes 0.000 description 3
- 102220516952 Core-binding factor subunit beta_T47N_mutation Human genes 0.000 description 3
- 102220492756 Cystatin-M_S31W_mutation Human genes 0.000 description 3
- 102220486711 Cytochrome b-245 chaperone 1_R44K_mutation Human genes 0.000 description 3
- QNAYBMKLOCPYGJ-UWTATZPHSA-N D-alanine Chemical compound C[C@@H](N)C(O)=O QNAYBMKLOCPYGJ-UWTATZPHSA-N 0.000 description 3
- 241000702421 Dependoparvovirus Species 0.000 description 3
- 241000588724 Escherichia coli Species 0.000 description 3
- 102220628160 FERM and PDZ domain-containing protein 4_P10W_mutation Human genes 0.000 description 3
- 241000282326 Felis catus Species 0.000 description 3
- 241000223221 Fusarium oxysporum Species 0.000 description 3
- 102220543314 Glucagon-like peptide 1 receptor_Q76A_mutation Human genes 0.000 description 3
- 102220485671 Glycophorin-A_T47K_mutation Human genes 0.000 description 3
- NYHBQMYGNKIUIF-UUOKFMHZSA-N Guanosine Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O NYHBQMYGNKIUIF-UUOKFMHZSA-N 0.000 description 3
- 108010031792 IGF Type 2 Receptor Proteins 0.000 description 3
- 102220487760 Isoaspartyl peptidase/L-asparaginase_E39R_mutation Human genes 0.000 description 3
- CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 description 3
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 description 3
- AGPKZVBTJJNPAG-WHFBIAKZSA-N L-isoleucine Chemical compound CC[C@H](C)[C@H](N)C(O)=O AGPKZVBTJJNPAG-WHFBIAKZSA-N 0.000 description 3
- ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 description 3
- COLNVLDHVKWLRT-QMMMGPOBSA-N L-phenylalanine Chemical compound OC(=O)[C@@H](N)CC1=CC=CC=C1 COLNVLDHVKWLRT-QMMMGPOBSA-N 0.000 description 3
- AYFVYJQAPQTCCC-GBXIJSLDSA-N L-threonine Chemical compound C[C@@H](O)[C@H](N)C(O)=O AYFVYJQAPQTCCC-GBXIJSLDSA-N 0.000 description 3
- OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 3
- 102000019218 Mannose-6-phosphate receptors Human genes 0.000 description 3
- 102220590298 Membrane-spanning 4-domains subfamily A member 14_P10H_mutation Human genes 0.000 description 3
- 102220482923 Mitochondrial tRNA-specific 2-thiouridylase 1_H84S_mutation Human genes 0.000 description 3
- 241000699666 Mus <mouse, genus> Species 0.000 description 3
- 241000699670 Mus sp. Species 0.000 description 3
- 108091034117 Oligonucleotide Proteins 0.000 description 3
- 239000002202 Polyethylene glycol Substances 0.000 description 3
- 102220543100 Protein pitchfork_T47L_mutation Human genes 0.000 description 3
- 108020004511 Recombinant DNA Proteins 0.000 description 3
- 102000005924 Triose-Phosphate Isomerase Human genes 0.000 description 3
- 108700015934 Triose-phosphate isomerases Proteins 0.000 description 3
- KZSNJWFQEVHDMF-UHFFFAOYSA-N Valine Natural products CC(C)C(N)C(O)=O KZSNJWFQEVHDMF-UHFFFAOYSA-N 0.000 description 3
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 3
- 238000002835 absorbance Methods 0.000 description 3
- 108010048241 acetamidase Proteins 0.000 description 3
- 238000007792 addition Methods 0.000 description 3
- 229960003767 alanine Drugs 0.000 description 3
- 102000004139 alpha-Amylases Human genes 0.000 description 3
- 108090000637 alpha-Amylases Proteins 0.000 description 3
- 229940024171 alpha-amylase Drugs 0.000 description 3
- 230000027455 binding Effects 0.000 description 3
- 239000012148 binding buffer Substances 0.000 description 3
- 102220369180 c.115G>A Human genes 0.000 description 3
- 102220426871 c.117G>C Human genes 0.000 description 3
- 102220348252 c.131G>C Human genes 0.000 description 3
- 102220407716 c.154G>A Human genes 0.000 description 3
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 3
- 230000008859 change Effects 0.000 description 3
- 239000012228 culture supernatant Substances 0.000 description 3
- 210000004748 cultured cell Anatomy 0.000 description 3
- 230000007812 deficiency Effects 0.000 description 3
- 229940079593 drug Drugs 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 239000000284 extract Substances 0.000 description 3
- 230000012010 growth Effects 0.000 description 3
- 230000002209 hydrophobic effect Effects 0.000 description 3
- 238000000338 in vitro Methods 0.000 description 3
- 230000003834 intracellular effect Effects 0.000 description 3
- 210000003734 kidney Anatomy 0.000 description 3
- 238000011068 loading method Methods 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 230000001404 mediated effect Effects 0.000 description 3
- 239000002609 medium Substances 0.000 description 3
- 239000002777 nucleoside Substances 0.000 description 3
- 210000000056 organ Anatomy 0.000 description 3
- 229920001223 polyethylene glycol Polymers 0.000 description 3
- 230000002265 prevention Effects 0.000 description 3
- 230000017854 proteolysis Effects 0.000 description 3
- 238000011002 quantification Methods 0.000 description 3
- 230000010076 replication Effects 0.000 description 3
- 230000000717 retained effect Effects 0.000 description 3
- 102220210519 rs1057524253 Human genes 0.000 description 3
- 102220212704 rs1060501388 Human genes 0.000 description 3
- 102220335295 rs1162744670 Human genes 0.000 description 3
- 102200011634 rs121908293 Human genes 0.000 description 3
- 102220039525 rs139161723 Human genes 0.000 description 3
- 102220068412 rs139166382 Human genes 0.000 description 3
- 102200062902 rs139401671 Human genes 0.000 description 3
- 102220044567 rs142243299 Human genes 0.000 description 3
- 102220082236 rs143549737 Human genes 0.000 description 3
- 102220204397 rs150199231 Human genes 0.000 description 3
- 102220249871 rs1553637237 Human genes 0.000 description 3
- 102220249788 rs1553687863 Human genes 0.000 description 3
- 102200010841 rs1553925558 Human genes 0.000 description 3
- 102220282285 rs1555570500 Human genes 0.000 description 3
- 102200000769 rs193922748 Human genes 0.000 description 3
- 102220020331 rs199472702 Human genes 0.000 description 3
- 102220184840 rs199513213 Human genes 0.000 description 3
- 102200087468 rs2070956 Human genes 0.000 description 3
- 102220160065 rs373524235 Human genes 0.000 description 3
- 102220186302 rs374913902 Human genes 0.000 description 3
- 102220013969 rs397516851 Human genes 0.000 description 3
- 102220028756 rs398123245 Human genes 0.000 description 3
- 102200129877 rs587777365 Human genes 0.000 description 3
- 102220045637 rs587782266 Human genes 0.000 description 3
- 102220137179 rs755230402 Human genes 0.000 description 3
- 102220272596 rs759505297 Human genes 0.000 description 3
- 102200072523 rs794726859 Human genes 0.000 description 3
- 102220077467 rs797044943 Human genes 0.000 description 3
- 102220264742 rs864622263 Human genes 0.000 description 3
- 102220099573 rs878853712 Human genes 0.000 description 3
- 102220156241 rs886061834 Human genes 0.000 description 3
- 230000028327 secretion Effects 0.000 description 3
- 230000035945 sensitivity Effects 0.000 description 3
- 239000002904 solvent Substances 0.000 description 3
- 239000000725 suspension Substances 0.000 description 3
- 238000002560 therapeutic procedure Methods 0.000 description 3
- 239000003981 vehicle Substances 0.000 description 3
- 239000013603 viral vector Substances 0.000 description 3
- GRGNVOCPFLXGDQ-TWHXEDJUSA-N (2r,3r,4s,5r,6r)-2-[(2r,3r,4r,5r,6s)-6-[(2r,3s,4r,5r,6r)-6-[(e,2s,3r)-2-amino-3-hydroxyoctadec-4-enoxy]-4,5-dihydroxy-2-(hydroxymethyl)oxan-3-yl]oxy-4,5-dihydroxy-2-(hydroxymethyl)oxan-3-yl]oxy-6-(hydroxymethyl)oxane-3,4,5-triol Chemical compound O[C@@H]1[C@@H](O)[C@H](OC[C@H](N)[C@H](O)/C=C/CCCCCCCCCCCCC)O[C@H](CO)[C@H]1O[C@H]1[C@H](O)[C@@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](O)[C@@H](O)[C@@H](CO)O2)O)[C@@H](CO)O1 GRGNVOCPFLXGDQ-TWHXEDJUSA-N 0.000 description 2
- OSJPPGNTCRNQQC-UWTATZPHSA-N 3-phospho-D-glyceric acid Chemical compound OC(=O)[C@H](O)COP(O)(O)=O OSJPPGNTCRNQQC-UWTATZPHSA-N 0.000 description 2
- VDABVNMGKGUPEY-UHFFFAOYSA-N 6-carboxyfluorescein succinimidyl ester Chemical compound C=1C(O)=CC=C2C=1OC1=CC(O)=CC=C1C2(C1=C2)OC(=O)C1=CC=C2C(=O)ON1C(=O)CCC1=O VDABVNMGKGUPEY-UHFFFAOYSA-N 0.000 description 2
- 102000007698 Alcohol dehydrogenase Human genes 0.000 description 2
- 108010021809 Alcohol dehydrogenase Proteins 0.000 description 2
- 102100034044 All-trans-retinol dehydrogenase [NAD(+)] ADH1B Human genes 0.000 description 2
- 101710193111 All-trans-retinol dehydrogenase [NAD(+)] ADH4 Proteins 0.000 description 2
- 108010037870 Anthranilate Synthase Proteins 0.000 description 2
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 2
- 108700010070 Codon Usage Proteins 0.000 description 2
- 241000699802 Cricetulus griseus Species 0.000 description 2
- QNAYBMKLOCPYGJ-UHFFFAOYSA-N D-alpha-Ala Natural products CC([NH3+])C([O-])=O QNAYBMKLOCPYGJ-UHFFFAOYSA-N 0.000 description 2
- 239000012591 Dulbecco’s Phosphate Buffered Saline Substances 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- 102000010911 Enzyme Precursors Human genes 0.000 description 2
- 108010062466 Enzyme Precursors Proteins 0.000 description 2
- 101000834253 Gallus gallus Actin, cytoplasmic 1 Proteins 0.000 description 2
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 2
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 2
- 229930186217 Glycolipid Natural products 0.000 description 2
- 102000003886 Glycoproteins Human genes 0.000 description 2
- 108090000288 Glycoproteins Proteins 0.000 description 2
- DCXYFEDJOCDNAF-REOHCLBHSA-N L-asparagine Chemical compound OC(=O)[C@@H](N)CC(N)=O DCXYFEDJOCDNAF-REOHCLBHSA-N 0.000 description 2
- QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 description 2
- KZSNJWFQEVHDMF-BYPYZUCNSA-N L-valine Chemical compound CC(C)[C@H](N)C(O)=O KZSNJWFQEVHDMF-BYPYZUCNSA-N 0.000 description 2
- ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 description 2
- 102000004882 Lipase Human genes 0.000 description 2
- 108090001060 Lipase Proteins 0.000 description 2
- 239000004367 Lipase Substances 0.000 description 2
- 241000124008 Mammalia Species 0.000 description 2
- SBKRTALNRRAOJP-BWSIXKJUSA-N N-[(2S)-4-amino-1-[[(2S,3R)-1-[[(2S)-4-amino-1-oxo-1-[[(3S,6S,9S,12S,15R,18R,21S)-6,9,18-tris(2-aminoethyl)-15-benzyl-3-[(1R)-1-hydroxyethyl]-12-(2-methylpropyl)-2,5,8,11,14,17,20-heptaoxo-1,4,7,10,13,16,19-heptazacyclotricos-21-yl]amino]butan-2-yl]amino]-3-hydroxy-1-oxobutan-2-yl]amino]-1-oxobutan-2-yl]-6-methylheptanamide (6S)-N-[(2S)-4-amino-1-[[(2S,3R)-1-[[(2S)-4-amino-1-oxo-1-[[(3S,6S,9S,12S,15R,18R,21S)-6,9,18-tris(2-aminoethyl)-15-benzyl-3-[(1R)-1-hydroxyethyl]-12-(2-methylpropyl)-2,5,8,11,14,17,20-heptaoxo-1,4,7,10,13,16,19-heptazacyclotricos-21-yl]amino]butan-2-yl]amino]-3-hydroxy-1-oxobutan-2-yl]amino]-1-oxobutan-2-yl]-6-methyloctanamide sulfuric acid Chemical compound OS(O)(=O)=O.CC(C)CCCCC(=O)N[C@@H](CCN)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCN)C(=O)N[C@H]1CCNC(=O)[C@@H](NC(=O)[C@H](CCN)NC(=O)[C@H](CCN)NC(=O)[C@H](CC(C)C)NC(=O)[C@@H](Cc2ccccc2)NC(=O)[C@@H](CCN)NC1=O)[C@@H](C)O.CC[C@H](C)CCCCC(=O)N[C@@H](CCN)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCN)C(=O)N[C@H]1CCNC(=O)[C@@H](NC(=O)[C@H](CCN)NC(=O)[C@H](CCN)NC(=O)[C@H](CC(C)C)NC(=O)[C@@H](Cc2ccccc2)NC(=O)[C@@H](CCN)NC1=O)[C@@H](C)O SBKRTALNRRAOJP-BWSIXKJUSA-N 0.000 description 2
- XJLXINKUBYWONI-NNYOXOHSSA-N NADP zwitterion Chemical compound NC(=O)C1=CC=C[N+]([C@H]2[C@@H]([C@H](O)[C@@H](COP([O-])(=O)OP(O)(=O)OC[C@@H]3[C@H]([C@@H](OP(O)(O)=O)[C@@H](O3)N3C4=NC=NC(N)=C4N=C3)O)O2)O)=C1 XJLXINKUBYWONI-NNYOXOHSSA-N 0.000 description 2
- 241000283973 Oryctolagus cuniculus Species 0.000 description 2
- 239000004695 Polyether sulfone Substances 0.000 description 2
- 108010093965 Polymyxin B Proteins 0.000 description 2
- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
- 229920002472 Starch Polymers 0.000 description 2
- 229930006000 Sucrose Natural products 0.000 description 2
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 2
- 108700005078 Synthetic Genes Proteins 0.000 description 2
- AYFVYJQAPQTCCC-UHFFFAOYSA-N Threonine Natural products CC(O)C(N)C(O)=O AYFVYJQAPQTCCC-UHFFFAOYSA-N 0.000 description 2
- 239000004473 Threonine Substances 0.000 description 2
- IQFYYKKMVGJFEH-XLPZGREQSA-N Thymidine Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](CO)[C@@H](O)C1 IQFYYKKMVGJFEH-XLPZGREQSA-N 0.000 description 2
- QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 description 2
- DRTQHJPVMGBUCF-XVFCMESISA-N Uridine Chemical compound O[C@@H]1[C@H](O)[C@@H](CO)O[C@H]1N1C(=O)NC(=O)C=C1 DRTQHJPVMGBUCF-XVFCMESISA-N 0.000 description 2
- IXKSXJFAGXLQOQ-XISFHERQSA-N WHWLQLKPGQPMY Chemical compound C([C@@H](C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC(C)C)C(=O)N1CCC[C@H]1C(=O)NCC(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC(O)=O)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(O)=O)NC(=O)[C@@H](N)CC=1C2=CC=CC=C2NC=1)C1=CNC=N1 IXKSXJFAGXLQOQ-XISFHERQSA-N 0.000 description 2
- 238000009825 accumulation Methods 0.000 description 2
- 239000008186 active pharmaceutical agent Substances 0.000 description 2
- OIRDTQYFTABQOQ-KQYNXXCUSA-N adenosine Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O OIRDTQYFTABQOQ-KQYNXXCUSA-N 0.000 description 2
- 239000002671 adjuvant Substances 0.000 description 2
- 235000004279 alanine Nutrition 0.000 description 2
- 238000013459 approach Methods 0.000 description 2
- 125000003118 aryl group Chemical group 0.000 description 2
- 230000004071 biological effect Effects 0.000 description 2
- 210000004204 blood vessel Anatomy 0.000 description 2
- 108010006025 bovine growth hormone Proteins 0.000 description 2
- 229910052799 carbon Inorganic materials 0.000 description 2
- 238000012219 cassette mutagenesis Methods 0.000 description 2
- 230000006037 cell lysis Effects 0.000 description 2
- 238000002659 cell therapy Methods 0.000 description 2
- 238000004587 chromatography analysis Methods 0.000 description 2
- 230000000295 complement effect Effects 0.000 description 2
- 239000000356 contaminant Substances 0.000 description 2
- 239000013078 crystal Substances 0.000 description 2
- 239000005549 deoxyribonucleoside Substances 0.000 description 2
- 230000000378 dietary effect Effects 0.000 description 2
- 239000003085 diluting agent Substances 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 208000035475 disorder Diseases 0.000 description 2
- 239000000839 emulsion Substances 0.000 description 2
- 230000012202 endocytosis Effects 0.000 description 2
- 238000002641 enzyme replacement therapy Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000012091 fetal bovine serum Substances 0.000 description 2
- 230000006870 function Effects 0.000 description 2
- 125000000524 functional group Chemical group 0.000 description 2
- 150000002339 glycosphingolipids Chemical class 0.000 description 2
- 230000013595 glycosylation Effects 0.000 description 2
- 238000006206 glycosylation reaction Methods 0.000 description 2
- 210000005003 heart tissue Anatomy 0.000 description 2
- HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 description 2
- 210000005260 human cell Anatomy 0.000 description 2
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 2
- 239000007943 implant Substances 0.000 description 2
- 238000001727 in vivo Methods 0.000 description 2
- 229960004717 insulin aspart Drugs 0.000 description 2
- 229960000310 isoleucine Drugs 0.000 description 2
- AGPKZVBTJJNPAG-UHFFFAOYSA-N isoleucine Natural products CCC(C)C(N)C(O)=O AGPKZVBTJJNPAG-UHFFFAOYSA-N 0.000 description 2
- BPHPUYQFMNQIOC-NXRLNHOXSA-N isopropyl beta-D-thiogalactopyranoside Chemical compound CC(C)S[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O BPHPUYQFMNQIOC-NXRLNHOXSA-N 0.000 description 2
- 229960003136 leucine Drugs 0.000 description 2
- 235000019421 lipase Nutrition 0.000 description 2
- 150000002632 lipids Chemical class 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 238000012544 monitoring process Methods 0.000 description 2
- 238000010172 mouse model Methods 0.000 description 2
- VOMXSOIBEJBQNF-UTTRGDHVSA-N novorapid Chemical compound C([C@H](NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CO)NC(=O)[C@H](CS)NC(=O)[C@H]([C@@H](C)CC)NC(=O)[C@H](CO)NC(=O)[C@H]([C@@H](C)O)NC(=O)[C@H](CS)NC(=O)[C@H](CS)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](C(C)C)NC(=O)[C@@H](NC(=O)CN)[C@@H](C)CC)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N[C@@H](CS)C(=O)N[C@@H](CC(N)=O)C(O)=O)C1=CC=C(O)C=C1.C([C@@H](C(=O)N[C@@H](CC(C)C)C(=O)N[C@H](C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CS)C(=O)NCC(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)NCC(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H]([C@@H](C)O)C(O)=O)C(C)C)NC(=O)[C@H](CO)NC(=O)CNC(=O)[C@H](CS)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC=1NC=NC=1)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CC(N)=O)NC(=O)[C@@H](NC(=O)[C@@H](N)CC=1C=CC=CC=1)C(C)C)C1=CN=CN1 VOMXSOIBEJBQNF-UTTRGDHVSA-N 0.000 description 2
- 125000003835 nucleoside group Chemical group 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 230000035764 nutrition Effects 0.000 description 2
- 230000037361 pathway Effects 0.000 description 2
- 239000008188 pellet Substances 0.000 description 2
- 210000003819 peripheral blood mononuclear cell Anatomy 0.000 description 2
- COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Natural products OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 description 2
- 150000008300 phosphoramidites Chemical class 0.000 description 2
- ZWLUXSQADUDCSB-UHFFFAOYSA-N phthalaldehyde Chemical compound O=CC1=CC=CC=C1C=O ZWLUXSQADUDCSB-UHFFFAOYSA-N 0.000 description 2
- 229920006393 polyether sulfone Polymers 0.000 description 2
- 229960003548 polymyxin b sulfate Drugs 0.000 description 2
- 230000001124 posttranscriptional effect Effects 0.000 description 2
- 239000003755 preservative agent Substances 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 230000000069 prophylactic effect Effects 0.000 description 2
- 210000005084 renal tissue Anatomy 0.000 description 2
- 230000002441 reversible effect Effects 0.000 description 2
- 239000002342 ribonucleoside Substances 0.000 description 2
- 230000003248 secreting effect Effects 0.000 description 2
- 150000003384 small molecules Chemical class 0.000 description 2
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 2
- 238000000527 sonication Methods 0.000 description 2
- UNFWWIHTNXNPBV-WXKVUWSESA-N spectinomycin Chemical compound O([C@@H]1[C@@H](NC)[C@@H](O)[C@H]([C@@H]([C@H]1O1)O)NC)[C@]2(O)[C@H]1O[C@H](C)CC2=O UNFWWIHTNXNPBV-WXKVUWSESA-N 0.000 description 2
- 239000003381 stabilizer Substances 0.000 description 2
- 238000010561 standard procedure Methods 0.000 description 2
- 235000019698 starch Nutrition 0.000 description 2
- 239000012536 storage buffer Substances 0.000 description 2
- 229960004793 sucrose Drugs 0.000 description 2
- 239000013589 supplement Substances 0.000 description 2
- 238000003786 synthesis reaction Methods 0.000 description 2
- 238000010189 synthetic method Methods 0.000 description 2
- 238000003146 transient transfection Methods 0.000 description 2
- 230000032258 transport Effects 0.000 description 2
- 230000001810 trypsinlike Effects 0.000 description 2
- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 2
- 239000004474 valine Substances 0.000 description 2
- 229960004295 valine Drugs 0.000 description 2
- 230000003612 virological effect Effects 0.000 description 2
- 238000005303 weighing Methods 0.000 description 2
- 210000005253 yeast cell Anatomy 0.000 description 2
- MTCFGRXMJLQNBG-REOHCLBHSA-N (2S)-2-Amino-3-hydroxypropansäure Chemical compound OC[C@H](N)C(O)=O MTCFGRXMJLQNBG-REOHCLBHSA-N 0.000 description 1
- LNAZSHAWQACDHT-XIYTZBAFSA-N (2r,3r,4s,5r,6s)-4,5-dimethoxy-2-(methoxymethyl)-3-[(2s,3r,4s,5r,6r)-3,4,5-trimethoxy-6-(methoxymethyl)oxan-2-yl]oxy-6-[(2r,3r,4s,5r,6r)-4,5,6-trimethoxy-2-(methoxymethyl)oxan-3-yl]oxyoxane Chemical compound CO[C@@H]1[C@@H](OC)[C@H](OC)[C@@H](COC)O[C@H]1O[C@H]1[C@H](OC)[C@@H](OC)[C@H](O[C@H]2[C@@H]([C@@H](OC)[C@H](OC)O[C@@H]2COC)OC)O[C@@H]1COC LNAZSHAWQACDHT-XIYTZBAFSA-N 0.000 description 1
- UHDGCWIWMRVCDJ-UHFFFAOYSA-N 1-beta-D-Xylofuranosyl-NH-Cytosine Natural products O=C1N=C(N)C=CN1C1C(O)C(O)C(CO)O1 UHDGCWIWMRVCDJ-UHFFFAOYSA-N 0.000 description 1
- 101710163881 5,6-dihydroxyindole-2-carboxylic acid oxidase Proteins 0.000 description 1
- 244000171897 Acacia nilotica subsp nilotica Species 0.000 description 1
- 102000007469 Actins Human genes 0.000 description 1
- 108010085238 Actins Proteins 0.000 description 1
- GUBGYTABKSRVRQ-XLOQQCSPSA-N Alpha-Lactose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@H](O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-XLOQQCSPSA-N 0.000 description 1
- 241000272525 Anas platyrhynchos Species 0.000 description 1
- DCXYFEDJOCDNAF-UHFFFAOYSA-N Asparagine Natural products OC(=O)C(N)CC(N)=O DCXYFEDJOCDNAF-UHFFFAOYSA-N 0.000 description 1
- 101000961203 Aspergillus awamori Glucoamylase Proteins 0.000 description 1
- 101000690713 Aspergillus niger Alpha-glucosidase Proteins 0.000 description 1
- 240000006439 Aspergillus oryzae Species 0.000 description 1
- 235000002247 Aspergillus oryzae Nutrition 0.000 description 1
- 241000416162 Astragalus gummifer Species 0.000 description 1
- 238000000035 BCA protein assay Methods 0.000 description 1
- 101000950981 Bacillus subtilis (strain 168) Catabolic NAD-specific glutamate dehydrogenase RocG Proteins 0.000 description 1
- DWRXFEITVBNRMK-UHFFFAOYSA-N Beta-D-1-Arabinofuranosylthymine Natural products O=C1NC(=O)C(C)=CN1C1C(O)C(O)C(CO)O1 DWRXFEITVBNRMK-UHFFFAOYSA-N 0.000 description 1
- 102000006734 Beta-Globulins Human genes 0.000 description 1
- 108010087504 Beta-Globulins Proteins 0.000 description 1
- 102100030981 Beta-alanine-activating enzyme Human genes 0.000 description 1
- 239000002126 C01EB10 - Adenosine Substances 0.000 description 1
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
- 241000282472 Canis lupus familiaris Species 0.000 description 1
- 108010059892 Cellulase Proteins 0.000 description 1
- 241000282693 Cercopithecidae Species 0.000 description 1
- 102000008186 Collagen Human genes 0.000 description 1
- 108010035532 Collagen Proteins 0.000 description 1
- 208000032170 Congenital Abnormalities Diseases 0.000 description 1
- 101710199851 Copy number protein Proteins 0.000 description 1
- 208000006069 Corneal Opacity Diseases 0.000 description 1
- MIKUYHXYGGJMLM-GIMIYPNGSA-N Crotonoside Natural products C1=NC2=C(N)NC(=O)N=C2N1[C@H]1O[C@@H](CO)[C@H](O)[C@@H]1O MIKUYHXYGGJMLM-GIMIYPNGSA-N 0.000 description 1
- 244000303965 Cyamopsis psoralioides Species 0.000 description 1
- UHDGCWIWMRVCDJ-PSQAKQOGSA-N Cytidine Natural products O=C1N=C(N)C=CN1[C@@H]1[C@@H](O)[C@@H](O)[C@H](CO)O1 UHDGCWIWMRVCDJ-PSQAKQOGSA-N 0.000 description 1
- 102000018832 Cytochromes Human genes 0.000 description 1
- 108010052832 Cytochromes Proteins 0.000 description 1
- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 description 1
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 1
- 150000008574 D-amino acids Chemical group 0.000 description 1
- NYHBQMYGNKIUIF-UHFFFAOYSA-N D-guanosine Natural products C1=2NC(N)=NC(=O)C=2N=CN1C1OC(CO)C(O)C1O NYHBQMYGNKIUIF-UHFFFAOYSA-N 0.000 description 1
- 244000000626 Daucus carota Species 0.000 description 1
- 235000002767 Daucus carota Nutrition 0.000 description 1
- 206010011878 Deafness Diseases 0.000 description 1
- 241000255581 Drosophila <fruit fly, genus> Species 0.000 description 1
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 1
- 241001302584 Escherichia coli str. K-12 substr. W3110 Species 0.000 description 1
- 241000206602 Eukaryota Species 0.000 description 1
- 101150108358 GLAA gene Proteins 0.000 description 1
- 102000048120 Galactokinases Human genes 0.000 description 1
- 108700023157 Galactokinases Proteins 0.000 description 1
- 108010046992 Galactosylgalactosylglucosylceramidase Proteins 0.000 description 1
- 108010010803 Gelatin Proteins 0.000 description 1
- 101150014526 Gla gene Proteins 0.000 description 1
- 102000016901 Glutamate dehydrogenase Human genes 0.000 description 1
- 239000004471 Glycine Substances 0.000 description 1
- 102000005744 Glycoside Hydrolases Human genes 0.000 description 1
- 108010031186 Glycoside Hydrolases Proteins 0.000 description 1
- 101150009006 HIS3 gene Proteins 0.000 description 1
- 101100295959 Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1) arcB gene Proteins 0.000 description 1
- 101100246753 Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1) pyrF gene Proteins 0.000 description 1
- 102100027685 Hemoglobin subunit alpha Human genes 0.000 description 1
- 108091005902 Hemoglobin subunit alpha Proteins 0.000 description 1
- 108091005904 Hemoglobin subunit beta Proteins 0.000 description 1
- 102100021519 Hemoglobin subunit beta Human genes 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 241000282412 Homo Species 0.000 description 1
- 101000773364 Homo sapiens Beta-alanine-activating enzyme Proteins 0.000 description 1
- 101000664737 Homo sapiens Somatotropin Proteins 0.000 description 1
- 241001480714 Humicola insolens Species 0.000 description 1
- 241001562081 Ikeda Species 0.000 description 1
- 108060003951 Immunoglobulin Proteins 0.000 description 1
- 102100027612 Kallikrein-11 Human genes 0.000 description 1
- SNDPXSYFESPGGJ-BYPYZUCNSA-N L-2-aminopentanoic acid Chemical compound CCC[C@H](N)C(O)=O SNDPXSYFESPGGJ-BYPYZUCNSA-N 0.000 description 1
- QUOGESRFPZDMMT-UHFFFAOYSA-N L-Homoarginine Natural products OC(=O)C(N)CCCCNC(N)=N QUOGESRFPZDMMT-UHFFFAOYSA-N 0.000 description 1
- AHLPHDHHMVZTML-BYPYZUCNSA-N L-Ornithine Chemical compound NCCC[C@H](N)C(O)=O AHLPHDHHMVZTML-BYPYZUCNSA-N 0.000 description 1
- HNDVDQJCIGZPNO-YFKPBYRVSA-N L-histidine Chemical compound OC(=O)[C@@H](N)CC1=CN=CN1 HNDVDQJCIGZPNO-YFKPBYRVSA-N 0.000 description 1
- QUOGESRFPZDMMT-YFKPBYRVSA-N L-homoarginine Chemical compound OC(=O)[C@@H](N)CCCCNC(N)=N QUOGESRFPZDMMT-YFKPBYRVSA-N 0.000 description 1
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 1
- SNDPXSYFESPGGJ-UHFFFAOYSA-N L-norVal-OH Natural products CCCC(N)C(O)=O SNDPXSYFESPGGJ-UHFFFAOYSA-N 0.000 description 1
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 1
- 208000031942 Late Onset disease Diseases 0.000 description 1
- 108091026898 Leader sequence (mRNA) Proteins 0.000 description 1
- 241000288904 Lemur Species 0.000 description 1
- 241000713666 Lentivirus Species 0.000 description 1
- 208000000501 Lipidoses Diseases 0.000 description 1
- 206010024585 Lipidosis Diseases 0.000 description 1
- 239000006137 Luria-Bertani broth Substances 0.000 description 1
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 1
- 239000004472 Lysine Substances 0.000 description 1
- 101150068888 MET3 gene Proteins 0.000 description 1
- 229930195725 Mannitol Natural products 0.000 description 1
- 108010014251 Muramidase Proteins 0.000 description 1
- 102000016943 Muramidase Human genes 0.000 description 1
- HSHXDCVZWHOWCS-UHFFFAOYSA-N N'-hexadecylthiophene-2-carbohydrazide Chemical compound CCCCCCCCCCCCCCCCNNC(=O)c1cccs1 HSHXDCVZWHOWCS-UHFFFAOYSA-N 0.000 description 1
- 108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 description 1
- 101100022915 Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) cys-11 gene Proteins 0.000 description 1
- 244000061176 Nicotiana tabacum Species 0.000 description 1
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 1
- 108090000913 Nitrate Reductases Proteins 0.000 description 1
- 108700026244 Open Reading Frames Proteins 0.000 description 1
- AHLPHDHHMVZTML-UHFFFAOYSA-N Orn-delta-NH2 Natural products NCCCC(N)C(O)=O AHLPHDHHMVZTML-UHFFFAOYSA-N 0.000 description 1
- UTJLXEIPEHZYQJ-UHFFFAOYSA-N Ornithine Natural products OC(=O)C(C)CCCN UTJLXEIPEHZYQJ-UHFFFAOYSA-N 0.000 description 1
- 102100028200 Ornithine transcarbamylase, mitochondrial Human genes 0.000 description 1
- 101710113020 Ornithine transcarbamylase, mitochondrial Proteins 0.000 description 1
- 102100037214 Orotidine 5'-phosphate decarboxylase Human genes 0.000 description 1
- 108010055012 Orotidine-5'-phosphate decarboxylase Proteins 0.000 description 1
- 241000700629 Orthopoxvirus Species 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 208000002193 Pain Diseases 0.000 description 1
- 102000011755 Phosphoglycerate Kinase Human genes 0.000 description 1
- 108091000080 Phosphotransferase Proteins 0.000 description 1
- 241000235648 Pichia Species 0.000 description 1
- 102000001253 Protein Kinase Human genes 0.000 description 1
- 241000700159 Rattus Species 0.000 description 1
- 208000001647 Renal Insufficiency Diseases 0.000 description 1
- 241000235403 Rhizomucor miehei Species 0.000 description 1
- 101100394989 Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009) hisI gene Proteins 0.000 description 1
- 241000283984 Rodentia Species 0.000 description 1
- 101900354623 Saccharomyces cerevisiae Galactokinase Proteins 0.000 description 1
- 101100022918 Schizosaccharomyces pombe (strain 972 / ATCC 24843) sua1 gene Proteins 0.000 description 1
- MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 description 1
- 102100032491 Serine protease 1 Human genes 0.000 description 1
- 108020004682 Single-Stranded DNA Proteins 0.000 description 1
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 1
- 244000061456 Solanum tuberosum Species 0.000 description 1
- 235000002595 Solanum tuberosum Nutrition 0.000 description 1
- 241000256248 Spodoptera Species 0.000 description 1
- 108091081024 Start codon Proteins 0.000 description 1
- 101100370749 Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) trpC1 gene Proteins 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 1
- 241000223258 Thermomyces lanuginosus Species 0.000 description 1
- 101001099217 Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) Triosephosphate isomerase Proteins 0.000 description 1
- 108091036066 Three prime untranslated region Proteins 0.000 description 1
- 208000009205 Tinnitus Diseases 0.000 description 1
- 229920001615 Tragacanth Polymers 0.000 description 1
- 108700019146 Transgenes Proteins 0.000 description 1
- 208000032109 Transient ischaemic attack Diseases 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- 244000098338 Triticum aestivum Species 0.000 description 1
- 101710152431 Trypsin-like protease Proteins 0.000 description 1
- 101150050575 URA3 gene Proteins 0.000 description 1
- 108091023045 Untranslated Region Proteins 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 241000212749 Zesius chrysomallus Species 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- DPXJVFZANSGRMM-UHFFFAOYSA-N acetic acid;2,3,4,5,6-pentahydroxyhexanal;sodium Chemical compound [Na].CC(O)=O.OCC(O)C(O)C(O)C(O)C=O DPXJVFZANSGRMM-UHFFFAOYSA-N 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 229960005305 adenosine Drugs 0.000 description 1
- 230000001464 adherent effect Effects 0.000 description 1
- 239000000443 aerosol Substances 0.000 description 1
- 108010056760 agalsidase beta Proteins 0.000 description 1
- 230000002776 aggregation Effects 0.000 description 1
- 238000004220 aggregation Methods 0.000 description 1
- 125000001931 aliphatic group Chemical group 0.000 description 1
- 230000000172 allergic effect Effects 0.000 description 1
- VREFGVBLTWBCJP-UHFFFAOYSA-N alprazolam Chemical compound C12=CC(Cl)=CC=C2N2C(C)=NN=C2CN=C1C1=CC=CC=C1 VREFGVBLTWBCJP-UHFFFAOYSA-N 0.000 description 1
- 230000002052 anaphylactic effect Effects 0.000 description 1
- 210000004102 animal cell Anatomy 0.000 description 1
- 238000010171 animal model Methods 0.000 description 1
- 101150008194 argB gene Proteins 0.000 description 1
- 125000000637 arginyl group Chemical group N[C@@H](CCCNC(N)=N)C(=O)* 0.000 description 1
- 210000004507 artificial chromosome Anatomy 0.000 description 1
- 229960001230 asparagine Drugs 0.000 description 1
- 235000009582 asparagine Nutrition 0.000 description 1
- 229940009098 aspartate Drugs 0.000 description 1
- 235000003704 aspartic acid Nutrition 0.000 description 1
- 208000010668 atopic eczema Diseases 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 108010051210 beta-Fructofuranosidase Proteins 0.000 description 1
- IQFYYKKMVGJFEH-UHFFFAOYSA-N beta-L-thymidine Natural products O=C1NC(=O)C(C)=CN1C1OC(CO)C(O)C1 IQFYYKKMVGJFEH-UHFFFAOYSA-N 0.000 description 1
- DRTQHJPVMGBUCF-PSQAKQOGSA-N beta-L-uridine Natural products O[C@H]1[C@@H](O)[C@H](CO)O[C@@H]1N1C(=O)NC(=O)C=C1 DRTQHJPVMGBUCF-PSQAKQOGSA-N 0.000 description 1
- OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 description 1
- 238000002306 biochemical method Methods 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 239000003139 biocide Substances 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000036983 biotransformation Effects 0.000 description 1
- 230000017531 blood circulation Effects 0.000 description 1
- 210000000133 brain stem Anatomy 0.000 description 1
- 239000001110 calcium chloride Substances 0.000 description 1
- 229910001628 calcium chloride Inorganic materials 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
- 239000001768 carboxy methyl cellulose Substances 0.000 description 1
- 239000003054 catalyst Substances 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 239000006143 cell culture medium Substances 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 229940106157 cellulase Drugs 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 210000001175 cerebrospinal fluid Anatomy 0.000 description 1
- 208000026106 cerebrovascular disease Diseases 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 235000015218 chewing gum Nutrition 0.000 description 1
- 210000004978 chinese hamster ovary cell Anatomy 0.000 description 1
- 230000002759 chromosomal effect Effects 0.000 description 1
- 210000000349 chromosome Anatomy 0.000 description 1
- 238000003776 cleavage reaction Methods 0.000 description 1
- 229920001436 collagen Polymers 0.000 description 1
- 239000002299 complementary DNA Substances 0.000 description 1
- 238000004590 computer program Methods 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000013270 controlled release Methods 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 231100000269 corneal opacity Toxicity 0.000 description 1
- 239000006071 cream Substances 0.000 description 1
- 239000003431 cross linking reagent Substances 0.000 description 1
- UHDGCWIWMRVCDJ-ZAKLUEHWSA-N cytidine Chemical compound O=C1N=C(N)C=CN1[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O1 UHDGCWIWMRVCDJ-ZAKLUEHWSA-N 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000001934 delay Effects 0.000 description 1
- 230000002939 deleterious effect Effects 0.000 description 1
- 238000002716 delivery method Methods 0.000 description 1
- 238000001212 derivatisation Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 239000003599 detergent Substances 0.000 description 1
- 235000007882 dietary composition Nutrition 0.000 description 1
- 235000013681 dietary sucrose Nutrition 0.000 description 1
- 208000013829 diffuse lymphatic malformation Diseases 0.000 description 1
- 229940079919 digestives enzyme preparation Drugs 0.000 description 1
- 235000021186 dishes Nutrition 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 239000008298 dragée Substances 0.000 description 1
- 238000004520 electroporation Methods 0.000 description 1
- KUBARPMUNHKBIQ-VTHUDJRQSA-N eliglustat tartrate Chemical compound OC(=O)[C@H](O)[C@@H](O)C(O)=O.C([C@@H](NC(=O)CCCCCCC)[C@H](O)C=1C=C2OCCOC2=CC=1)N1CCCC1.C([C@@H](NC(=O)CCCCCCC)[C@H](O)C=1C=C2OCCOC2=CC=1)N1CCCC1 KUBARPMUNHKBIQ-VTHUDJRQSA-N 0.000 description 1
- 239000003974 emollient agent Substances 0.000 description 1
- 239000003995 emulsifying agent Substances 0.000 description 1
- OYFJQPXVCSSHAI-QFPUQLAESA-N enalapril maleate Chemical compound OC(=O)\C=C/C(O)=O.C([C@@H](C(=O)OCC)N[C@@H](C)C(=O)N1[C@@H](CCC1)C(O)=O)CC1=CC=CC=C1 OYFJQPXVCSSHAI-QFPUQLAESA-N 0.000 description 1
- 210000001163 endosome Anatomy 0.000 description 1
- 239000003623 enhancer Substances 0.000 description 1
- 239000003797 essential amino acid Substances 0.000 description 1
- 235000020776 essential amino acid Nutrition 0.000 description 1
- 229940014516 fabrazyme Drugs 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 239000000945 filler Substances 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 238000001917 fluorescence detection Methods 0.000 description 1
- 239000007850 fluorescent dye Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000004927 fusion Effects 0.000 description 1
- 108020001507 fusion proteins Proteins 0.000 description 1
- 102000037865 fusion proteins Human genes 0.000 description 1
- 125000002519 galactosyl group Chemical group C1([C@H](O)[C@@H](O)[C@@H](O)[C@H](O1)CO)* 0.000 description 1
- 230000007160 gastrointestinal dysfunction Effects 0.000 description 1
- 238000001502 gel electrophoresis Methods 0.000 description 1
- 239000008273 gelatin Substances 0.000 description 1
- 229920000159 gelatin Polymers 0.000 description 1
- 235000019322 gelatine Nutrition 0.000 description 1
- 235000011852 gelatine desserts Nutrition 0.000 description 1
- 229930195712 glutamate Natural products 0.000 description 1
- 235000013922 glutamic acid Nutrition 0.000 description 1
- 239000004220 glutamic acid Substances 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 229940029575 guanosine Drugs 0.000 description 1
- 230000010370 hearing loss Effects 0.000 description 1
- 231100000888 hearing loss Toxicity 0.000 description 1
- 208000016354 hearing loss disease Diseases 0.000 description 1
- 208000019622 heart disease Diseases 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 229910001385 heavy metal Inorganic materials 0.000 description 1
- 238000013537 high throughput screening Methods 0.000 description 1
- 238000004191 hydrophobic interaction chromatography Methods 0.000 description 1
- 239000001866 hydroxypropyl methyl cellulose Substances 0.000 description 1
- 235000010979 hydroxypropyl methyl cellulose Nutrition 0.000 description 1
- 229920003088 hydroxypropyl methyl cellulose Polymers 0.000 description 1
- 108010002685 hygromycin-B kinase Proteins 0.000 description 1
- 230000028993 immune response Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 102000018358 immunoglobulin Human genes 0.000 description 1
- 230000000415 inactivating effect Effects 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 239000007972 injectable composition Substances 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 230000010189 intracellular transport Effects 0.000 description 1
- 238000007913 intrathecal administration Methods 0.000 description 1
- 238000001990 intravenous administration Methods 0.000 description 1
- 239000001573 invertase Substances 0.000 description 1
- 235000011073 invertase Nutrition 0.000 description 1
- 238000004255 ion exchange chromatography Methods 0.000 description 1
- 235000015110 jellies Nutrition 0.000 description 1
- 201000006370 kidney failure Diseases 0.000 description 1
- 239000008101 lactose Substances 0.000 description 1
- 230000000670 limiting effect Effects 0.000 description 1
- 239000000865 liniment Substances 0.000 description 1
- 238000001638 lipofection Methods 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 208000018773 low birth weight Diseases 0.000 description 1
- 239000007937 lozenge Substances 0.000 description 1
- 101150039489 lysZ gene Proteins 0.000 description 1
- 239000012139 lysis buffer Substances 0.000 description 1
- 229960000274 lysozyme Drugs 0.000 description 1
- 239000004325 lysozyme Substances 0.000 description 1
- 235000010335 lysozyme Nutrition 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 239000000594 mannitol Substances 0.000 description 1
- 235000010355 mannitol Nutrition 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000007721 medicinal effect Effects 0.000 description 1
- 201000001441 melanoma Diseases 0.000 description 1
- 238000002844 melting Methods 0.000 description 1
- 230000008018 melting Effects 0.000 description 1
- MYWUZJCMWCOHBA-VIFPVBQESA-N methamphetamine Chemical compound CN[C@@H](C)CC1=CC=CC=C1 MYWUZJCMWCOHBA-VIFPVBQESA-N 0.000 description 1
- 229930182817 methionine Natural products 0.000 description 1
- HOVAGTYPODGVJG-ZFYZTMLRSA-N methyl alpha-D-glucopyranoside Chemical compound CO[C@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O HOVAGTYPODGVJG-ZFYZTMLRSA-N 0.000 description 1
- HOVAGTYPODGVJG-VEIUFWFVSA-N methyl alpha-D-mannoside Chemical compound CO[C@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]1O HOVAGTYPODGVJG-VEIUFWFVSA-N 0.000 description 1
- HOVAGTYPODGVJG-UHFFFAOYSA-N methyl beta-galactoside Natural products COC1OC(CO)C(O)C(O)C1O HOVAGTYPODGVJG-UHFFFAOYSA-N 0.000 description 1
- 229920000609 methyl cellulose Polymers 0.000 description 1
- 239000001923 methylcellulose Substances 0.000 description 1
- 235000010981 methylcellulose Nutrition 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000003094 microcapsule Substances 0.000 description 1
- 239000004005 microsphere Substances 0.000 description 1
- 231100000219 mutagenic Toxicity 0.000 description 1
- 230000003505 mutagenic effect Effects 0.000 description 1
- 208000010125 myocardial infarction Diseases 0.000 description 1
- UPSFMJHZUCSEHU-JYGUBCOQSA-N n-[(2s,3r,4r,5s,6r)-2-[(2r,3s,4r,5r,6s)-5-acetamido-4-hydroxy-2-(hydroxymethyl)-6-(4-methyl-2-oxochromen-7-yl)oxyoxan-3-yl]oxy-4,5-dihydroxy-6-(hydroxymethyl)oxan-3-yl]acetamide Chemical compound CC(=O)N[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@H]1[C@H](O)[C@@H](NC(C)=O)[C@H](OC=2C=C3OC(=O)C=C(C)C3=CC=2)O[C@@H]1CO UPSFMJHZUCSEHU-JYGUBCOQSA-N 0.000 description 1
- 239000002088 nanocapsule Substances 0.000 description 1
- 239000002086 nanomaterial Substances 0.000 description 1
- 239000002071 nanotube Substances 0.000 description 1
- 210000000653 nervous system Anatomy 0.000 description 1
- 208000004296 neuralgia Diseases 0.000 description 1
- 208000021722 neuropathic pain Diseases 0.000 description 1
- 101150095344 niaD gene Proteins 0.000 description 1
- 229930027945 nicotinamide-adenine dinucleotide Natural products 0.000 description 1
- 239000002736 nonionic surfactant Substances 0.000 description 1
- 150000003833 nucleoside derivatives Chemical class 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 239000002674 ointment Substances 0.000 description 1
- 229960003104 ornithine Drugs 0.000 description 1
- 108090000021 oryzin Proteins 0.000 description 1
- 230000036407 pain Effects 0.000 description 1
- 239000003973 paint Substances 0.000 description 1
- 238000002638 palliative care Methods 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 239000006072 paste Substances 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- 208000033808 peripheral neuropathy Diseases 0.000 description 1
- 239000012071 phase Substances 0.000 description 1
- JTJMJGYZQZDUJJ-UHFFFAOYSA-N phencyclidine Chemical compound C1CCCCN1C1(C=2C=CC=CC=2)CCCCC1 JTJMJGYZQZDUJJ-UHFFFAOYSA-N 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 108010082527 phosphinothricin N-acetyltransferase Proteins 0.000 description 1
- 230000026731 phosphorylation Effects 0.000 description 1
- 238000006366 phosphorylation reaction Methods 0.000 description 1
- 102000020233 phosphotransferase Human genes 0.000 description 1
- 239000006187 pill Substances 0.000 description 1
- 229920000136 polysorbate Polymers 0.000 description 1
- 239000013641 positive control Substances 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 230000002028 premature Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000000750 progressive effect Effects 0.000 description 1
- 238000002731 protein assay Methods 0.000 description 1
- 108060006633 protein kinase Proteins 0.000 description 1
- 238000001742 protein purification Methods 0.000 description 1
- 230000002797 proteolythic effect Effects 0.000 description 1
- 210000001938 protoplast Anatomy 0.000 description 1
- 101150054232 pyrG gene Proteins 0.000 description 1
- 238000010791 quenching Methods 0.000 description 1
- 230000000171 quenching effect Effects 0.000 description 1
- 238000002708 random mutagenesis Methods 0.000 description 1
- 239000000376 reactant Substances 0.000 description 1
- 239000011541 reaction mixture Substances 0.000 description 1
- 102000005962 receptors Human genes 0.000 description 1
- 108020003175 receptors Proteins 0.000 description 1
- 238000010188 recombinant method Methods 0.000 description 1
- 238000005215 recombination Methods 0.000 description 1
- 230000006798 recombination Effects 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 238000004366 reverse phase liquid chromatography Methods 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 238000005185 salting out Methods 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 230000007017 scission Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000002864 sequence alignment Methods 0.000 description 1
- 239000012679 serum free medium Substances 0.000 description 1
- 230000001568 sexual effect Effects 0.000 description 1
- 239000000377 silicon dioxide Substances 0.000 description 1
- 238000002741 site-directed mutagenesis Methods 0.000 description 1
- 210000003491 skin Anatomy 0.000 description 1
- 206010040882 skin lesion Diseases 0.000 description 1
- 231100000444 skin lesion Toxicity 0.000 description 1
- 239000002002 slurry Substances 0.000 description 1
- 239000001632 sodium acetate Substances 0.000 description 1
- 235000017281 sodium acetate Nutrition 0.000 description 1
- 235000019812 sodium carboxymethyl cellulose Nutrition 0.000 description 1
- 239000001488 sodium phosphate Substances 0.000 description 1
- 229910000162 sodium phosphate Inorganic materials 0.000 description 1
- 238000010532 solid phase synthesis reaction Methods 0.000 description 1
- 239000000600 sorbitol Substances 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 238000012289 standard assay Methods 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 235000000346 sugar Nutrition 0.000 description 1
- 230000003319 supportive effect Effects 0.000 description 1
- 239000000829 suppository Substances 0.000 description 1
- 230000035900 sweating Effects 0.000 description 1
- 230000002194 synthesizing effect Effects 0.000 description 1
- 239000006188 syrup Substances 0.000 description 1
- 235000020357 syrup Nutrition 0.000 description 1
- 230000009885 systemic effect Effects 0.000 description 1
- 239000003826 tablet Substances 0.000 description 1
- 230000008685 targeting Effects 0.000 description 1
- 229940124597 therapeutic agent Drugs 0.000 description 1
- 229940104230 thymidine Drugs 0.000 description 1
- 231100000886 tinnitus Toxicity 0.000 description 1
- 230000000699 topical effect Effects 0.000 description 1
- 239000000196 tragacanth Substances 0.000 description 1
- 235000010487 tragacanth Nutrition 0.000 description 1
- 229940116362 tragacanth Drugs 0.000 description 1
- 230000005030 transcription termination Effects 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 201000010875 transient cerebral ischemia Diseases 0.000 description 1
- RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 description 1
- GPRLSGONYQIRFK-MNYXATJNSA-N triton Chemical compound [3H+] GPRLSGONYQIRFK-MNYXATJNSA-N 0.000 description 1
- 101150016309 trpC gene Proteins 0.000 description 1
- 238000005199 ultracentrifugation Methods 0.000 description 1
- 238000011144 upstream manufacturing Methods 0.000 description 1
- DRTQHJPVMGBUCF-UHFFFAOYSA-N uracil arabinoside Natural products OC1C(O)C(CO)OC1N1C(=O)NC(=O)C=C1 DRTQHJPVMGBUCF-UHFFFAOYSA-N 0.000 description 1
- 229940045145 uridine Drugs 0.000 description 1
- 230000001018 virulence Effects 0.000 description 1
- 239000011653 vitamin D2 Substances 0.000 description 1
- MECHNRXZTMCUDQ-RKHKHRCZSA-N vitamin D2 Chemical compound C1(/[C@@H]2CC[C@@H]([C@]2(CCC1)C)[C@H](C)/C=C/[C@H](C)C(C)C)=C\C=C1\C[C@@H](O)CCC1=C MECHNRXZTMCUDQ-RKHKHRCZSA-N 0.000 description 1
- 235000001892 vitamin D2 Nutrition 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 230000036266 weeks of gestation Effects 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2465—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1) acting on alpha-galactose-glycoside bonds, e.g. alpha-galactosidase (3.2.1.22)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/43—Enzymes; Proenzymes; Derivatives thereof
- A61K38/46—Hydrolases (3)
- A61K38/47—Hydrolases (3) acting on glycosyl compounds (3.2), e.g. cellulases, lactases
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01022—Alpha-galactosidase (3.2.1.22)
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Pharmacology & Pharmacy (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Gastroenterology & Hepatology (AREA)
- Immunology (AREA)
- Epidemiology (AREA)
- Obesity (AREA)
- Hematology (AREA)
- Diabetes (AREA)
- Enzymes And Modification Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicinal Preparation (AREA)
Abstract
본 발명은 조작된 인간 알파-갈락토시다제 폴리펩타이드 및 이의 조성물을 제공한다. 조작된 인간 알파-갈락토시다제 폴리펩타이드는 개선된 열안정성, 혈청 안정성, 개선된 세포 흡수, 산성(pH <4) 조건과 염기성(pH >7) 조건의 양 조건 하에서의 안정성, 감소된 면역원성, 및 세포로부터의 개선된 글로보트리아오실세라마이드 제거를 제공하도록 최적화되었다. 또한, 본 발명은 치료 목적을 위한 상기 조작된 인간 알파-갈락토시다제 폴리펩타이드를 포함하는 조성물의 용도에 관한 것이다.
Description
본 출원은 모든 목적을 위해 그 전문이 본원에 참조로 포함되는 2020년 2월 28일에 출원된 미국 가출원 일련 번호 제62/982,949호에 대한 우선권을 주장한다.
발명의 분야
본 발명은 조작된 인간 알파-갈락토시다제 폴리펩타이드 및 이의 조성물을 제공한다. 조작된 인간 알파-갈락토시다제 폴리펩타이드는 개선된 열안정성, 혈청 안정성, 감소된 면역원성, 개선된 세포 흡수, 산성(pH <4) 조건과 염기성(pH >7) 조건의 양 조건 하에서의 안정성, 및 세포로부터의 개선된 글로보트리아오실세라마이드 제거를 제공하도록 최적화되었다. 또한, 본 발명은 치료 목적을 위한 상기 조작된 인간 알파-갈락토시다제 폴리펩타이드를 포함하는 조성물의 용도에 관한 것이다.
서열 목록, 표 또는 컴퓨터 프로그램에 대한 참조
서열 목록의 공식 사본은 EFS-웹(EFS-Web)을 통해 ASCII 포맷된 텍스트 파일(파일명: "CX7-203WO2_ST25.txt", 작성일: 2021년 2월 22일, 및 크기: 4.43 메가바이트)로서 본 명세서와 함께 동시에 제출된다. EFS-웹을 통해 제출된 서열 목록은 본 명세서의 일부이며, 그 전문이 본원에 참조로 포함된다.
인간 알파-갈락토시다제("GLA"; EC 3.2.1.22)는 당지질 및 당단백질로부터 말단 알파 갈락토실 모이어티를 가수분해하는 것을 담당하는 리소좀 당단백질이다. 이는 다양한 인간 조직에 존재하는 다수의 기질에 대해 작용한다. 파브리병(Fabry disease)(이는 또한 미만성 구간 혈관각화종, 앤더슨-파브리병, 유전성 이소성 지질증, 알파-갈락토시다제 A 결핍증, GLA 결핍증, 및 세라미드 트리헥소시다제 결핍증로도 지칭된다)은 알파-갈락토시다제 A 활성 결핍 또는 부재로부터 유발되는 글리코스핑고리피드 이화작용의 X-연관 선천성 이상이다. 파브리병에 걸린 환자에서는 혈관, 조직 및 기관의 혈장 및 세포 리소좀에 글로보트리아오실세라마이드(본원에서 "Gb3" 및 "Gb3"로 지칭됨) 및 관련된 글리코스핑고리피드가 축적된다(예를 들어, 문헌[Nance et al., Arch. Neurol., 63:453-457 [2006]] 참조). 환자가 노령화됨에 따라, 상기 지질의 축적에 기인하여 혈관은 점진적으로 좁아지게 되고, 이로써, 특히, 피부, 신장, 심장, 뇌, 및 신경계에서의 조직으로의 혈류 및 영양은 감소된다. 따라서, 파브리병은 신부전, 심장 질환, 뇌혈관 질환, 소섬유 말초 신경병증 및 피부 병변뿐만 아니라, 다른 장애로 나타나는 전신 장애이다(예를 들어, 문헌[Schiffmann, Pharm. Ther., 122:65-77 [2009]] 참조). 이환된 환자는 증상, 예컨대, 손발 통증, 환자 피부 상의 작은 암적색 반점 군집, 발한 능력 감소, 각막 혼탁, 위장 기능 장애, 이명 및 청력 손실을 보인다. 잠재적으로 생명을 위협하는 합병증으로는 진행성 신장 손상, 심장마비 및 뇌졸중을 포함한다. 상기 질환은 남성 40,000-60,000명 중 1명꼴로 이환되는 것으로 추정되며, 이는 또한 여성에서도 발생한다. 실제로, 파브리병을 앓는 이형접합성 여성은 신경계 이상, 만성 통증, 피로감, 고혈압, 심장 질환, 신부전증, 및 뇌졸중을 비롯하고, 이에 따라 의학적 치료가 필요한, 상당한 생명 위협성 병태를 경험한다(예를 들어, 문헌[Want et al., Genet. Med., 13:457-484 [2011]] 참조). 파브리병 징후는 유아기때부터 어느 시점에서든 시작될 수 있으며, 비록 일부 환자는 좀더 경미한 후기 발병 질환을 보이기도 하지만, 징후는 보통 4세 내지 8세 사이에 나타나기 시작한다. 치료는 일반적으로 지지 치료이며, 파브리병에 대한 치유 방법은 없는 바, 이에, 안전하고, 효과적인 치료법이 여전히 요구되고 있다.
본 발명은 조작된 인간 알파-갈락토시다제 폴리펩타이드 및 그의 조성물을 제공한다. 조작된 인간 알파-갈락토시다제 폴리펩타이드는 개선된 열안정성, 혈청 안정성, 감소된 면역원성, 개선된 세포 흡수, 및 산성(pH <4) 조건과 염기성(pH >7) 조건의 양 조건 하에서의 안정성, 및 세포로부터의 개선된 글로보트리아오실세라마이드 제거를 제공하도록 최적화되었다. 또한, 본 발명은 치료 목적을 위한 상기 조작된 인간 알파-갈락토시다제 폴리펩타이드를 포함하는 조성물의 용도에 관한 것이다.
본 발명은 서열번호(SEQ ID NO) 8에 대해 적어도 약 70%, 적어도 약 75%, 적어도 약 80%, 적어도 약 85%, 적어도 약 90%, 적어도 약 91%, 적어도 약 92%, 적어도 약 93%, 적어도 약 94%, 적어도 약 95%, 적어도 약 96%, 적어도 약 97%, 적어도 약 98%, 또는 적어도 약 99%의 서열 동일성을 포함하는 아미노산 서열을 포함하는 재조합 알파-갈락토시다제 A 및/또는 생물학적으로 활성인 재조합 알파-갈락토시다제 A 단편을 제공한다. 본 발명은 서열번호 8에 대해 적어도 70%, 적어도 75%, 적어도 80%, 적어도 85%, 적어도 90%, 적어도 91%, 적어도 92%, 적어도 93%, 적어도 94%, 적어도 95%, 적어도 96%, 적어도 97%, 적어도 98%, 또는 적어도 99%의 서열 동일성을 포함하는 아미노산 서열을 포함하는 재조합 알파-갈락토시다제 A 및/또는 생물학적으로 활성인 재조합 알파-갈락토시다제 A 단편을 제공한다.
본 발명은 또한 서열번호 8에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 이상의 서열 동일성을 가지는 폴리펩타이드 서열 또는 이의 기능적 단편을 포함하는 재조합 알파-갈락토시다제 A를 제공하고, 여기서, 상기 재조합 알파-갈락토시다제 A는 44, 44/217, 44/217/316, 44/217/322, 44/217/322/337, 44/247, 44/247/302, 44/247/302/322, 44/247/322, 44/247/337, 44/247/362, 44/302, 44/337, 44/373, 217/322, 217/373, 247/322, 247/362, 302/322/362/373, 302/337, 316, 316/337, 322, 322/337, 362/373 및 373으로부터 선택된 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열번호 8을 기준으로 넘버링된다. 일부 실시양태에서, 재조합 알파-갈락토시다제 A는 서열번호 8에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 이상의 서열 동일성을 가지는 폴리펩타이드 서열 또는 이의 기능적 단편을 포함하고, 여기서, 상기 재조합 알파-갈락토시다제 A는 44L, 44L/217F, 44L/217F/316L, 44L/217F/322M, 44L/217F/322M/337A, 44L/247N, 44L/247N/302Q, 44L/247N/302Q/322M, 44L/247N/322M, 44L/247N/337A, 44L/247N/362K, 44L/302Q, 44L/337A, 44L/373R, 217F/322M, 217F/373R, 247N/322M, 247N/362K, 302Q/322M/362K/373R, 302Q/337A, 316L, 316L/337A, 322M, 322M/337A, 362K/373R 및 373R로부터 선택된 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열번호 8을 기준으로 넘버링된다. 일부 실시양태에서, 재조합 알파-갈락토시다제 A는 서열번호 8에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 이상의 서열 동일성을 가지는 폴리펩타이드 서열 또는 이의 기능적 단편을 포함하고, 여기서, 상기 재조합 알파-갈락토시다제 A는 R44L, R44L/R217F, R44L/R217F/D316L, R44L/R217F/I322M, R44L/R217F/I322M/P337A, R44L/D247N, R44L/D247N/K302Q, R44L/D247N/K302Q/I322M, R44L/D247N/I322M, R44L/D247N/P337A, R44L/D247N/Q362K, R44L/K302Q, R44L/P337A, R44L/K373R, R217F/I322M, R217F/K373R, D247N/I322M, D247N/Q362K, K302Q/I322M/Q362K/K373R, K302Q/P337A, D316L, D316L/P337A, I322M, I322M/P337A, Q362K/K373R 및 K373R로부터 선택된 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열번호 8을 기준으로 넘버링된다.
본 발명은 또한 서열번호 8에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 이상의 서열 동일성을 가지는 폴리펩타이드 서열 또는 이의 기능적 단편을 포함하는 재조합 알파 글리코시다제 A를 제공하고, 여기서, 상기 재조합 알파-갈락토시다제 A는 10/39/44/47/92/166/206/217/247/261/271/302/316/322/337/362/368/373/392, 44/217/316, 44/217/322/337, 166/362, 217/373 및 362/373으로부터 선택된 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열번호 8을 기준으로 넘버링된다. 일부 실시양태에서, 재조합 알파-갈락토시다제 A는 서열번호 8에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 이상의 서열 동일성을 가지는 폴리펩타이드 서열 또는 이의 기능적 단편을 포함하고, 여기서, 상기 재조합 알파-갈락토시다제 A는 10T/39M/44L/47S/92Y/166S/206K/217F/247N/261A/271H/302Q/316L/322M/337A/362K/368W/373R/392M, 44L/217F/316L, 44L/217F/322M/337A, 166A/362K, 217F/373R 및 362K/373R로부터 선택된 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열번호 8을 기준으로 넘버링된다. 일부 실시양태에서, 재조합 알파-갈락토시다제 A는 서열번호 8에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 이상의 서열 동일성을 가지는 폴리펩타이드 서열 또는 이의 기능적 단편을 포함하고, 여기서, 상기 재조합 알파-갈락토시다제 A는 P10T/E39M/R44L/T47S/H92Y/P166S/A206K/R217F/D247N/G261A/A271H/K302Q/D316L/I322M/P337A/Q362K/A368W/K373R/T392M, R44L/R217F/D316L, R44L/R217F/I322M/P337A, P166A/Q362K, R217F/K373R 및 Q362K/K373R로부터 선택된 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열번호 8을 기준으로 넘버링된다.
본 발명은 또한 서열번호 58에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 이상의 서열 동일성을 가지는 폴리펩타이드 서열 또는 이의 기능적 단편을 포함하는 재조합 알파 갈락토시다제 A를 제공하고, 여기서, 상기 재조합 알파-갈락토시다제 A는 7, 7/48/68, 7/48/68/120/282/299, 7/48/130/282, 7/48/180, 7/68/130/282/365, 7/68/180, 7/88/120/305/365, 7/120, 7/130, 7/282, 7/305, 7/305/365, 7/365, 39, 47, 47/87/95/96/158/162, 47/95, 47/273, 47/343, 48, 48/68, 48/180/282, 48/282, 48/282/305, 67/180, 68, 68/299/300, 71, 87/91/95/96/158/162, 87/91/95/96/206/343, 87/96/155/273/343, 88, 91/95, 91/95/96, 92, 93, 96, 96/273, 96/312/343, 120, 120/299/305, 151, 158, 158/162/273, 162, 162/273, 162/343, 166, 178, 180, 181, 206, 217, 271, 273, 273/343, 282, 282/365, 293/391, 299/300, 299/300/305/365, 300, 301, 305, 305/365, 314, 333, 336, 337, 343, 345, 363, 365, 370, 389, 393, 394, 396/398, 397 및 398로부터 선택된 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열번호 58을 기준으로 넘버링된다. 일부 실시양태에서, 재조합 알파-갈락토시다제 A는 서열번호 58에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 이상의 서열 동일성을 가지는 폴리펩타이드 서열 또는 이의 기능적 단편을 포함하고, 여기서, 상기 재조합 알파-갈락토시다제 A는 7L, 7L/48D/68E, 7L/48D/68E/120H/282N/299R, 7L/48D/130E/282N, 7L/48D/180G, 7L/68E/130E/282N/365V, 7L/68E/180G, 7L/88A/120H/305G/365V, 7L/120H, 7L/130E, 7L/282N, 7L/305G, 7L/305G/365V, 7L/365V, 39V, 47D, 47D/87K/95E/96L/158R/162H, 47D/95E, 47D/273P, 47D/343G, 47V, 48D, 48D/68E, 48D/180G/282N, 48D/282N, 48D/282N/305G, 67T/180G, 68E, 68E/299R/300I, 71P, 87K/91Q/95E/96L/158A/162K, 87K/91Q/95E/96L/206S/343G, 87K/96I/155N/273P/343G, 88A, 91Q/95E, 91Q/95E/96L, 92F, 92T, 93I, 96L, 96L/273P, 96L/312Q/343G, 120H, 120H/299R/305G, 151L, 158A, 158A/162K/273G, 158R, 162H/343D, 162K, 162K/273P, 162S, 166K, 178G, 178S, 180G, 180L, 180T, 180V, 181A, 206K, 206S, 217K, 271R, 273P, 273P/343G, 282N, 282N/365V, 293P/391A, 299R/300I, 299R/300I/305G/365V, 300I, 301M, 305G, 305G/365V, 314A, 333F, 333G, 336V, 337R, 343D, 343G, 345A, 345Q, 363Q, 365A, 365Q, 365V, 370G, 389K, 393V, 394K, 396G/398T, 397A, 398A, 398P, 398S 및 398V로부터 선택된 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열번호 58을 기준으로 넘버링된다. 일부 실시양태에서, 재조합 알파-갈락토시다제 A는 서열번호 58에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 이상의 서열 동일성을 가지는 폴리펩타이드 서열 또는 이의 기능적 단편을 포함하고, 여기서, 상기 재조합 알파-갈락토시다제 A는 R7L, R7L/E48D/Q68E, R7L/E48D/Q68E/Y120H/D282N/Q299R, R7L/E48D/D130E/D282N, R7L/E48D/F180G, R7L/Q68E/D130E/D282N/F365V, R7L/Q68E/F180G, R7L/Q88A/Y120H/N305G/F365V, R7L/Y120H, R7L/D130E, R7L/D282N, R7L/N305G, R7L/N305G/F365V, R7L/F365V, E39V, T47D, T47D/R87K/S95E/K96L/L158R/R162H, T47D/S95E, T47D/S273P, T47D/K343G, T47V, E48D, E48D/Q68E, E48D/F180G/D282N, E48D/D282N, E48D/D282N/N305G, P67T/F180G, Q68E, Q68E/Q299R/L300I, S71P, R87K/N91Q/S95E/K96L/L158A/R162K, R87K/N91Q/S95E/K96L/A206S/K343G, R87K/K96I/H155N/S273P/K343G, Q88A, N91Q/S95E, N91Q/S95E/K96L, H92F, H92T, V93I, K96L, K96L/S273P, K96L/P312Q/K343G, Y120H, Y120H/Q299R/N305G, D151L, L158A, L158A/R162K/S273G, L158R, R162H/K343D, R162K, R162K/S273P, R162S, P166K, W178G, W178S, F180G, F180L, F180T, F180V, Q181A, A206K, A206S, R217K, A271R, S273P, S273P/K343G, D282N, D282N/F365V, L293P/Q391A, Q299R/L300I, Q299R/L300I/N305G/F365V, L300I, R301M, N305G, N305G/F365V, S314A, S333F, S333G, I336V, P337R, K343D, K343G, V345A, V345Q, L363Q, F365A, F365Q, F365V, S370G, T389K, S393V, L394K, D396G/L398T, L397A, L398A, L398P, L398S 및 L398V로부터 선택된 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열번호 58을 기준으로 넘버링된다.
본 발명은 또한 서열번호 158에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 이상의 서열 동일성을 가지는 폴리펩타이드 서열 또는 이의 기능적 단편을 포함하는 재조합 알파-갈락토시다제 A를 제공하고, 여기서, 상기 재조합 알파-갈락토시다제 A는 24/202, 39/47, 39/47/217, 39/151, 39/282/337/398, 39/337/343/398, 39/393/398, 47/130, 47/151, 47/343/345/393, 48, 48/68, 48/68/217/333/391/393, 48/68/333, 48/217, 48/333, 48/345/393, 48/393, 59/143, 68, 68/345, 130, 130/158, 130/158/393, 130/345/393, 143/271, 143/333, 143/387, 151, 151/158/217/343/345/393, 151/206/282/337/343/345/398, 151/282/393, 151/345/393/398, 151/393, 158, 158/393, 202, 206, 206/217, 217, 217/333, 217/337/345/398, 271, 282/393, 333, 333/345, 337/343/345/398, 343, 343/345/393/398, 393 및 393/398로부터 선택된 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열번호 158을 기준으로 넘버링된다. 일부 실시양태에서, 재조합 알파-갈락토시다제 A는 서열번호 158에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 이상의 서열 동일성을 가지는 폴리펩타이드 서열 또는 이의 기능적 단편을 포함하고, 여기서, 상기 재조합 알파-갈락토시다제 A는 24S/202N, 39V/47D, 39V/47V/217K, 39V/151L, 39V/282N/337R/398A, 39V/337R/343G/398A, 39V/393V/398A, 47V/130E, 47V/151L, 47V/343D/345Q/393V, 48D, 48D/68E, 48D/68E/217K/333F/391A/393V, 48D/68E/333F, 48D/217K, 48D/333F, 48D/333G, 48D/345Q/393V, 48D/393V, 59A/143S, 68E, 68E/345Q, 130E, 130E/158R, 130E/158R/393V, 130E/345Q/393V, 143S/271N, 143S/333N, 143S/387N, 151L, 151L/158R/217K/343G/345Q/393V, 151L/206S/282N/337R/343D/345Q/398A, 151L/282N/393V, 151L/345Q/393V/398A, 151L/393V, 158R, 158R/393V, 202N, 206S, 206S/217K, 217K, 217K/333F, 217K/333G, 217K/337R/345Q/398A, 271N, 282N/393V, 333F/345Q, 333G, 333N, 337R/343G/345Q/398A, 343D, 343D/345Q/393V/398A, 393V 및 393V/398A로부터 선택된 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열번호 158을 기준으로 넘버링된다. 일부 실시양태에서, 재조합 알파-갈락토시다제 A는 서열번호 8에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 이상의 서열 동일성을 가지는 폴리펩타이드 서열 또는 이의 기능적 단편을 포함하고, 여기서, 상기 재조합 알파-갈락토시다제 A는 D24S/D202N, E39V/T47D, E39V/T47V/R217K, E39V/D151L, E39V/D282N/P337R/L398A, E39V/P337R/K343G/L398A, E39V/S393V/L398A, T47V/D130E, T47V/D151L, T47V/K343D/V345Q/S393V, E48D, E48D/Q68E, E48D/Q68E/R217K/S333F/Q391A/S393V, E48D/Q68E/S333F, E48D/R217K, E48D/S333F, E48D/S333G, E48D/V345Q/S393V, E48D/S393V, C59A/C143S, Q68E, Q68E/V345Q, D130E, D130E/L158R, D130E/L158R/S393V, D130E/V345Q/S393V, C143S/A271N, C143S/S333N, C143S/E387N, D151L, D151L/L158R/R217K/K343G/V345Q/S393V, D151L/A206S/D282N/P337R/K343D/V345Q/L398A, D151L/D282N/S393V, D151L/V345Q/S393V/L398A, D151L/S393V, L158R, L158R/S393V, D202N, A206S, A206S/R217K, R217K, R217K/S333F, R217K/S333G, R217K/P337R/V345Q/L398A, A271N, D282N/S393V, S333F/V345Q, S333G, S333N, P337R/K343G/V345Q/L398A, K343D, K343D/V345Q/S393V/L398A, S393V 및 S393V/L398A로부터 선택된 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열번호 158을 기준으로 넘버링된다.
본 발명은 또한 서열번호 372에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 이상의 서열 동일성을 가지는 폴리펩타이드 서열 또는 이의 기능적 단편을 포함하는 재조합 알파-갈락토시다제 A를 제공하고, 여기서, 상기 재조합 알파-갈락토시다제 A는 10, 10/39/44/322, 10/39/92/206/217/271, 10/39/92/247, 10/39/92/247/271/316, 10/44, 10/44/47/92/247, 10/44/47/261/302/322/368, 10/44/92/316/322, 10/44/261/302/316, 10/44/302/337/368, 10/47/217/247/316/392, 10/47/217/322, 10/47/271, 10/92, 10/92/206/217/247, 10/92/206/247/316/322/392, 10/92/206/247/322/368, 10/92/217/261/302/337, 10/206/217/271, 10/206/247, 10/206/261/271/316, 10/261, 10/271/302, 10/302, 10/302/316, 10/302/322/337, 10/316/322, 10/337/392, 10/368, 39/44/92/162/247/302/316/322, 39/44/92/217/322, 39/44/92/247/271/302, 39/47/92/247/302/316/322, 39/47/217/247/368, 39/47/247, 39/92/247/302/316/337/368, 39/92/316/322, 39/247/271, 39/247/271/316, 39/322, 44/47/92/206/217/316/322, 44/47/92/247/261/271/316/337/368, 44/47/206/217/247/271/322, 44/47/247/322/368, 44/47/302/316/322, 44/92/206/247/368, 44/206/337, 44/247/261/302/316, 44/247/261/302/316/322, 47/92/247/271, 47/217/302, 47/247, 47/247/271, 89/217/247/261/302/316, 92/217/271, 92/247, 92/247/271/322, 92/247/302/322/337, 92/271/337, 92/302, 92/316, 206/217/271/392, 217/247/316/322/337/368, 247, 247/271, 247/302, 271, 271/302/322, 271/316/322, 302/322/368 및 368로부터 선택된 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열번호 372를 기준으로 넘버링된다. 일부 실시양태에서, 재조합 알파-갈락토시다제 A는 서열번호 372에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 이상의 서열 동일성을 가지는 폴리펩타이드 서열 또는 이의 기능적 단편을 포함하고, 여기서, 상기 재조합 알파-갈락토시다제 A는 10P, 10P/39E/44R/322I, 10P/39E/92H/206A/217R/271A, 10P/39E/92H/247D, 10P/39E/92H/247D/271A/316D, 10P/44R, 10P/44R/47T/92H/247D, 10P/44R/47T/261G/302K/322I/368A, 10P/44R/92H/316D/322I, 10P/44R/261G/302K/316D, 10P/44R/302K/337P/368A, 10P/47T/217R/247D/316D/392T, 10P/47T/217R/322I, 10P/47T/271A, 10P/92H, 10P/92H/206A/217R/247D, 10P/92H/206A/247D/316D/322I/392T, 10P/92H/206A/247D/322I/368A, 10P/92H/217R/261G/302K/337P, 10P/206A/217R/271A, 10P/206A/247D, 10P/206A/261G/271A/316D, 10P/261G, 10P/271A/302K, 10P/302K, 10P/302K/316D, 10P/302K/322I/337P, 10P/316D/322I, 10P/337P/392T, 10P/368A, 39E/44R/92H/162M/247D/302K/316D/322I, 39E/44R/92H/217R/322I, 39E/44R/92H/247D/271A/302K, 39E/47T/92H/247D/302K/316D/322I, 39E/47T/217R/247D/368A, 39E/47T/247D, 39E/92H/247D/302K/316D/337P/368A, 39E/92H/316D/322I, 39E/247D/271A, 39E/247D/271A/316D, 39E/322I, 44R/47T/92H/206A/217R/316D/322I, 44R/47T/92H/247D/261G/271A/316D/337P/368A, 44R/47T/206A/217R/247D/271A/322I, 44R/47T/247D/322I/368A, 44R/47T/302K/316D/322I, 44R/92H/206A/247D/368A, 44R/206A/337P, 44R/247D/261G/302K/316D, 44R/247D/261G/302K/316D/322I, 47T/92H/247D/271A, 47T/217R/302K, 47T/247D, 47T/247D/271A, 89I/217R/247D/261G/302K/316D, 92H/217R/271A, 92H/247D, 92H/247D/271A/322I, 92H/247D/302K/322I/337P, 92H/271A/337P, 92H/302K, 92H/316D, 206A/217R/271A/392T, 217R/247D/316D/322I/337P/368A, 247D, 247D/271A, 247D/302K, 271A, 271A/302K/322I, 271A/316D/322I, 302K/322I/368A 및 368A로부터 선택된 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열번호 372를 기준으로 넘버링된다. 일부 실시양태에서, 재조합 알파-갈락토시다제 A는 서열번호 372에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 이상의 서열 동일성을 가지는 폴리펩타이드 서열 또는 이의 기능적 단편을 포함하고, 여기서, 상기 재조합 알파-갈락토시다제 A는 T10P, T10P/M39E/L44R/M322I, T10P/M39E/Y92H/K206A/F217R/H271A, T10P/M39E/Y92H/N247D, T10P/M39E/Y92H/N247D/H271A/L316D, T10P/L44R, T10P/L44R/S47T/Y92H/N247D, T10P/L44R/S47T/A261G/Q302K/M322I/W368A, T10P/L44R/Y92H/L316D/M322I, T10P/L44R/A261G/Q302K/L316D, T10P/L44R/Q302K/A337P/W368A, T10P/S47T/F217R/N247D/L316D/M392T, T10P/S47T/F217R/M322I, T10P/S47T/H271A, T10P/Y92H, T10P/Y92H/K206A/F217R/N247D, T10P/Y92H/K206A/N247D/L316D/M322I/M392T, T10P/Y92H/K206A/N247D/M322I/W368A, T10P/Y92H/F217R/A261G/Q302K/A337P, T10P/K206A/F217R/H271A, T10P/K206A/N247D, T10P/K206A/A261G/H271A/L316D, T10P/A261G, T10P/H271A/Q302K, T10P/Q302K, T10P/Q302K/L316D, T10P/Q302K/M322I/A337P, T10P/L316D/M322I, T10P/A337P/M392T, T10P/W368A, M39E/L44R/Y92H/R162M/N247D/Q302K/L316D/M322I, M39E/L44R/Y92H/F217R/M322I, M39E/L44R/Y92H/N247D/H271A/Q302K, M39E/S47T/Y92H/N247D/Q302K/L316D/M322I, M39E/S47T/F217R/N247D/W368A, M39E/S47T/N247D, M39E/Y92H/N247D/Q302K/L316D/A337P/W368A, M39E/Y92H/L316D/M322I, M39E/N247D/H271A, M39E/N247D/H271A/L316D, M39E/M322I, L44R/S47T/Y92H/K206A/F217R/L316D/M322I, L44R/S47T/Y92H/N247D/A261G/H271A/L316D/A337P/W368A, L44R/S47T/K206A/F217R/N247D/H271A/M322I, L44R/S47T/N247D/M322I/W368A, L44R/S47T/Q302K/L316D/M322I, L44R/Y92H/K206A/N247D/W368A, L44R/K206A/A337P, L44R/N247D/A261G/Q302K/L316D, L44R/N247D/A261G/Q302K/L316D/M322I, S47T/Y92H/N247D/H271A, S47T/F217R/Q302K, S47T/N247D, S47T/N247D/H271A, L89I/F217R/N247D/A261G/Q302K/L316D, Y92H/F217R/H271A, Y92H/N247D, Y92H/N247D/H271A/M322I, Y92H/N247D/Q302K/M322I/A337P, Y92H/H271A/A337P, Y92H/Q302K, Y92H/L316D, K206A/F217R/H271A/M392T, F217R/N247D/L316D/M322I/A337P/W368A, N247D, N247D/H271A, N247D/Q302K, H271A, H271A/Q302K/M322I, H271A/L316D/M322I, Q302K/M322I/W368A 및 W368A로부터 선택된 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열번호 372를 기준으로 넘버링된다.
본 발명은 또한 서열번호 374에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 이상의 서열 동일성을 가지는 폴리펩타이드 서열 또는 이의 기능적 단편을 포함하는 재조합 알파-갈락토시다제 A를 제공하고, 여기서, 상기 재조합 알파-갈락토시다제 A는 10/36/92/166/247/261/316/392, 10/39, 10/39/44/47/92/206/217, 10/39/44/47/316, 10/39/44/47/337, 10/39/44/92/166/261/316/322, 10/39/44/92/166/302/322, 10/39/44/92/166/392, 10/39/44/92/217/302/322, 10/39/44/92/302/322, 10/39/44/166/261/271/316/322, 10/39/44/392, 10/39/47/92/337, 10/39/92/131/166/271/316/322, 10/39/92/166/217/247/271, 10/39/92/217/316, 10/44/47/166/261/271, 10/44/47/166/271/322/368, 10/44/47/217/271/316/322, 10/44/92, 10/44/92/217/247/271/302/316/392, 10/44/166/302, 10/44/206/316/322, 10/47/92/166/271/316/337, 10/47/92/271/302, 10/47/92/316/322/392, 10/47/166/271, 10/47/166/316, 10/92/166, 10/92/166/217/247/261/271, 10/92/166/261/271/392, 10/92/166/261/316/322/337, 10/92/166/337/368, 10/92/302/337, 10/92/316/322, 10/206, 10/206/247/261, 10/217/322, 10/261, 10/261/337/392, 10/316/392, 10/368, 39/44/47/92/166/206/392, 39/44/47/92/206/247/261, 39/44/47/92/206/392, 39/44/47/206/337/368/392, 39/44/92/166/247/261/302/337, 39/44/166/271, 39/44/166/271/337/368/392, 39/47/92/316/322, 39/47/92/392, 39/47/166/217/261/392, 39/47/217/247/368, 39/47/247, 39/92/166/217/392, 39/92/261/302, 39/166/217/261/316/368, 39/322, 39/392, 44/47, 44/47/92/217/271, 44/47/92/217/316/322/392, 44/47/92/392, 44/47/166, 44/47/166/271, 44/47/247/271/392, 44/316/322/392, 44/337, 47/166/206/217/247/337, 47/166/217/271/337, 47/206, 47/217/247/261, 47/271, 52/217/302/316, 92/166/206/271/316, 92/166/217/261/271/392, 92/166/217/316/337/392, 92/166/247, 92/166/316, 92/206/322, 92/217, 92/217/271/337, 92/261/271, 92/271, 166/217/316/322/337, 166/247/271/316, 166/316/322/337, 206/217, 217/392, 247/316, 316/322/368 및 316/337/392로부터 선택된 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열번호 374를 기준으로 넘버링된다. 일부 실시양태에서, 재조합 알파-갈락토시다제 A는 서열번호 374에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 이상의 서열 동일성을 가지는 폴리펩타이드 서열 또는 이의 기능적 단편을 포함하고, 여기서, 상기 재조합 알파-갈락토시다제 A는 10T/36M/92Y/166S/247N/261A/316L/392M, 10T/39M, 10T/39M/44L/47S/92Y/206K/217F, 10T/39M/44L/47S/316L, 10T/39M/44L/47S/337A, 10T/39M/44L/92Y/166S/261A/316L/322M, 10T/39M/44L/92Y/166S/302Q/322M, 10T/39M/44L/92Y/166S/392M, 10T/39M/44L/92Y/217F/302Q/322M, 10T/39M/44L/92Y/302Q/322M, 10T/39M/44L/166S/261A/271H/316L/322M, 10T/39M/44L/392M, 10T/39M/47S/92Y/337A, 10T/39M/92Y/131G/166S/271H/316L/322M, 10T/39M/92Y/166S/217F/247N/271H, 10T/39M/92Y/217F/316L, 10T/44L/47S/166S/261A/271H, 10T/44L/47S/166S/271H/322M/368W, 10T/44L/47S/217F/271H/316L/322M, 10T/44L/92Y, 10T/44L/92Y/217F/247N/271H/302Q/316L/392M, 10T/44L/166S/302Q, 10T/44L/206K/316L/322M, 10T/47S/92Y/166S/271H/316L/337A, 10T/47S/92Y/271H/302Q, 10T/47S/92Y/316L/322M/392M, 10T/47S/166S/271H, 10T/47S/166S/316L, 10T/92Y/166S, 10T/92Y/166S/217F/247N/261A/271H, 10T/92Y/166S/261A/271H/392M, 10T/92Y/166S/261A/316L/322M/337A, 10T/92Y/166S/337A/368W, 10T/92Y/302Q/337A, 10T/92Y/316L/322M, 10T/206K, 10T/206K/247N/261A, 10T/217F/322M, 10T/261A, 10T/261A/337A/392M, 10T/316L/392M, 10T/368W, 39M/44L/47S/92Y/166S/206K/392M, 39M/44L/47S/92Y/206K/247N/261A, 39M/44L/47S/92Y/206K/392M, 39M/44L/47S/206K/337A/368W/392M, 39M/44L/92Y/166S/247N/261A/302Q/337A, 39M/44L/166S/271H, 39M/44L/166S/271H/337A/368W/392M, 39M/47S/92Y/316L/322M, 39M/47S/92Y/392M, 39M/47S/166S/217F/261A/392M, 39M/47S/217F/247N/368W, 39M/47S/247N, 39M/92Y/166S/217F/392M, 39M/92Y/261A/302Q, 39M/166S/217F/261A/316L/368W, 39M/322M, 39M/392M, 44L/47S, 44L/47S/92Y/217F/271H, 44L/47S/92Y/217F/316L/322M/392M, 44L/47S/92Y/392M, 44L/47S/166S, 44L/47S/166S/271H, 44L/47S/247N/271H/392M, 44L/316L/322M/392M, 44L/337A, 47S/166S/206K/217F/247N/337A, 47S/166S/217F/271H/337A, 47S/206K, 47S/217F/247N/261A, 47S/271H, 52N/217F/302Q/316L, 92Y/166S/206K/271H/316L, 92Y/166S/217F/261A/271H/392M, 92Y/166S/217F/316L/337A/392M, 92Y/166S/247N, 92Y/166S/316L, 92Y/206K/322M, 92Y/217F, 92Y/217F/271H/337A, 92Y/261A/271H, 92Y/271H, 166S/217F/316L/322M/337A, 166S/247N/271H/316L, 166S/316L/322M/337A, 206K/217F, 217F/392M, 247N/316L, 316L/322M/368W 및 316L/337A/392M으로부터 선택된 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열번호 374를 기준으로 넘버링된다. 일부 실시양태에서, 재조합 알파-갈락토시다제 A는 서열번호 374에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 이상의 서열 동일성을 가지는 폴리펩타이드 서열 또는 이의 기능적 단편을 포함하고, 여기서, 상기 재조합 알파-갈락토시다제 A는 P10T/K36M/H92Y/P166S/D247N/G261A/D316L/T392M, P10T/E39M, P10T/E39M/R44L/T47S/H92Y/A206K/R217F, P10T/E39M/R44L/T47S/D316L, P10T/E39M/R44L/T47S/P337A, P10T/E39M/R44L/H92Y/P166S/G261A/D316L/I322M, P10T/E39M/R44L/H92Y/P166S/K302Q/I322M, P10T/E39M/R44L/H92Y/P166S/T392M, P10T/E39M/R44L/H92Y/R217F/K302Q/I322M, P10T/E39M/R44L/H92Y/K302Q/I322M, P10T/E39M/R44L/P166S/G261A/A271H/D316L/I322M, P10T/E39M/R44L/T392M, P10T/E39M/T47S/H92Y/P337A, P10T/E39M/H92Y/W131G/P166S/A271H/D316L/I322M, P10T/E39M/H92Y/P166S/R217F/D247N/A271H, P10T/E39M/H92Y/R217F/D316L, P10T/R44L/T47S/P166S/G261A/A271H, P10T/R44L/T47S/P166S/A271H/I322M/A368W, P10T/R44L/T47S/R217F/A271H/D316L/I322M, P10T/R44L/H92Y, P10T/R44L/H92Y/R217F/D247N/A271H/K302Q/D316L/T392M, P10T/R44L/P166S/K302Q, P10T/R44L/A206K/D316L/I322M, P10T/T47S/H92Y/P166S/A271H/D316L/P337A, P10T/T47S/H92Y/A271H/K302Q, P10T/T47S/H92Y/D316L/I322M/T392M, P10T/T47S/P166S/A271H, P10T/T47S/P166S/D316L, P10T/H92Y/P166S, P10T/H92Y/P166S/R217F/D247N/G261A/A271H, P10T/H92Y/P166S/G261A/A271H/T392M, P10T/H92Y/P166S/G261A/D316L/I322M/P337A, P10T/H92Y/P166S/P337A/A368W, P10T/H92Y/K302Q/P337A, P10T/H92Y/D316L/I322M, P10T/A206K, P10T/A206K/D247N/G261A, P10T/R217F/I322M, P10T/G261A, P10T/G261A/P337A/T392M, P10T/D316L/T392M, P10T/A368W, E39M/R44L/T47S/H92Y/P166S/A206K/T392M, E39M/R44L/T47S/H92Y/A206K/D247N/G261A, E39M/R44L/T47S/H92Y/A206K/T392M, E39M/R44L/T47S/A206K/P337A/A368W/T392M, E39M/R44L/H92Y/P166S/D247N/G261A/K302Q/P337A, E39M/R44L/P166S/A271H, E39M/R44L/P166S/A271H/P337A/A368W/T392M, E39M/T47S/H92Y/D316L/I322M, E39M/T47S/H92Y/T392M, E39M/T47S/P166S/R217F/G261A/T392M, E39M/T47S/R217F/D247N/A368W, E39M/T47S/D247N, E39M/H92Y/P166S/R217F/T392M, E39M/H92Y/G261A/K302Q, E39M/P166S/R217F/G261A/D316L/A368W, E39M/I322M, E39M/T392M, R44L/T47S, R44L/T47S/H92Y/R217F/A271H, R44L/T47S/H92Y/R217F/D316L/I322M/T392M, R44L/T47S/H92Y/T392M, R44L/T47S/P166S, R44L/T47S/P166S/A271H, R44L/T47S/D247N/A271H/T392M, R44L/D316L/I322M/T392M, R44L/P337A, T47S/P166S/A206K/R217F/D247N/P337A, T47S/P166S/R217F/A271H/P337A, T47S/A206K, T47S/R217F/D247N/G261A, T47S/A271H, D52N/R217F/K302Q/D316L, H92Y/P166S/A206K/A271H/D316L, H92Y/P166S/R217F/G261A/A271H/T392M, H92Y/P166S/R217F/D316L/P337A/T392M, H92Y/P166S/D247N, H92Y/P166S/D316L, H92Y/A206K/I322M, H92Y/R217F, H92Y/R217F/A271H/P337A, H92Y/G261A/A271H, H92Y/A271H, P166S/R217F/D316L/I322M/P337A, P166S/D247N/A271H/D316L, P166S/D316L/I322M/P337A, A206K/R217F, R217F/T392M, D247N/D316L, D316L/I322M/A368W 및 D316L/P337A/T392M으로부터 선택된 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열번호 374를 기준으로 넘버링된다.
본 발명은 또한 서열번호 704에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 이상의 서열 동일성을 가지는 폴리펩타이드 서열 또는 이의 기능적 단편을 포함하는 재조합 알파-갈락토시다제 A를 제공하고, 여기서, 상기 재조합 알파-갈락토시다제 A는 2, 4, 5, 24/59, 24/143/144, 24/143/202/333, 24/143/202/352/390/391, 24/143/333/352/387/390/391, 24/143/390/391, 24/202, 24/202/271, 24/202/333/352, 24/271/352, 24/352/387/390/391, 24/387/391, 31, 40, 59, 59/143, 59/143/202, 59/143/202/271/333, 59/143/271, 59/143/333, 59/202, 59/202/333, 59/271/387/390, 73, 76, 80, 83, 84, 91/215/361, 122, 123, 143, 143/202, 143/271, 143/271/352/390, 143/333, 143/333/387/390, 143/387/391, 147, 155, 164, 165, 179, 186, 202, 202/333, 210, 215/218, 218, 218/361, 218/361/398, 218/398, 246, 254/398, 271, 271/333, 271/333/390/391, 271/333/391, 271/352/391, 273, 275, 277, 278, 280, 281, 283, 284, 287, 300, 303, 304, 325, 331, 332, 333/352, 333/390/391, 333/391, 334, 335, 336, 338, 339, 340, 341, 343, 359, 360, 361, 362, 367, 369, 371, 373, 375, 377, 382, 382/398, 385, 387/391, 390 및 398로부터 선택된 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열번호 704를 기준으로 넘버링된다. 일부 실시양태에서, 재조합 알파-갈락토시다제 A는 서열번호 704에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 이상의 서열 동일성을 가지는 폴리펩타이드 서열 또는 이의 기능적 단편을 포함하고, 여기서, 상기 재조합 알파-갈락토시다제 A는 2S, 4L, 5M, 5V, 24S/59A, 24S/143S/144N, 24S/143S/202N/333N, 24S/143S/202N/352N/390N/391N, 24S/143S/333N/352N/387N/390T/391N, 24S/143S/390T/391N, 24S/202N, 24S/202N/271N, 24S/202N/333N/352N, 24S/271N/352N, 24S/352N/387N/390N/391N, 24S/387N/391N, 31F, 31H, 31L, 31T, 31W, 40Q, 59A, 59A/143S, 59A/143S/271N, 59A/202N, 59T, 59T/143S/202N, 59T/143S/333N, 59T/202N/333N, 59V/143S/202N/271N/333N, 59V/271N/387N/390T, 73A, 76A, 76F, 76M, 76S, 80T, 83R, 83S, 84G, 84K, 84R, 91S/215S/361T, 122E, 122N, 122S, 123Q, 123R, 123S, 123T, 143S, 143S/202N, 143S/271N, 143S/271N/352N/390N, 143S/333N, 143S/333N/387N/390T, 143S/387N/391N, 147L, 147S, 155A, 155D, 155F, 155L, 155R, 155T, 164E, 165I, 179H, 179L, 179R, 179W, 186E, 186F, 186M, 186P, 186R, 186S, 186Y, 202N, 202N/333N, 210I, 215S/218Y, 218Y, 218Y/361T, 218Y/361T/398F, 218Y/398F, 246Y, 254T/398F, 271N, 271N/333N, 271N/333N/390N/391N, 271N/333N/391N, 271N/352N/391N, 273L, 275A, 275G, 277Q, 277V, 278N, 278R, 278S, 280G, 281I, 281M, 283L, 283P, 283T, 283V, 284A, 284E, 284G, 284L, 284M, 284R, 284S, 287R, 300F, 303A, 303C, 303W, 304T, 304V, 304W, 325A, 331M, 332G, 332H, 333N/352N, 333N/390N/391N, 333N/390S/391N, 333N/391N, 334C, 334V, 335A, 335L, 336F, 336G, 336S, 336T, 338L, 339G, 339N, 339Q, 339V, 340H, 340I, 340K, 340M, 340P, 340W, 341F, 341M, 343L, 343R, 343S, 343W, 359F, 359L, 359R, 360H, 360V, 361T, 361V, 362H, 367A, 367D, 367L, 367M, 369D, 371G, 373L, 373S, 375L, 375Q, 377Q, 382I, 382I/398F, 385R, 387N/391N, 390S 및 398F로부터 선택된 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열번호 704를 기준으로 넘버링된다. 일부 실시양태에서, 재조합 알파-갈락토시다제 A는 서열번호 704에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 이상의 서열 동일성을 가지는 폴리펩타이드 서열 또는 이의 기능적 단편을 포함하고, 여기서, 상기 재조합 알파-갈락토시다제 A는 D2S, G4L, L5M, L5V, D24S/C59A, D24S/C143S/D144N, D24S/C143S/D202N/G333N, D24S/C143S/D202N/F352N/M390N/Q391N, D24S/C143S/G333N/F352N/E387N/M390T/Q391N, D24S/C143S/M390T/Q391N, D24S/D202N, D24S/D202N/A271N, D24S/D202N/G333N/F352N, D24S/A271N/F352N, D24S/F352N/E387N/M390N/Q391N, D24S/E387N/Q391N, S31F, S31H, S31L, S31T, S31W, E40Q, C59A, C59A/C143S, C59A/C143S/A271N, C59A/D202N, C59T, C59T/C143S/D202N, C59T/C143S/G333N, C59T/D202N/G333N, C59V/C143S/D202N/A271N/G333N, C59V/A271N/E387N/M390T, G73A, Q76A, Q76F, Q76M, Q76S, Q80T, P83R, P83S, H84G, H84K, H84R, N91S/T215S/R361T, D122E, D122N, D122S, I123Q, I123R, I123S, I123T, C143S, C143S/D202N, C143S/A271N, C143S/A271N/F352N/M390N, C143S/G333N, C143S/G333N/E387N/M390T, C143S/E387N/Q391N, E147L, E147S, H155A, H155D, H155F, H155L, H155R, H155T, G164E, R165I, P179H, P179L, P179R, P179W, T186E, T186F, T186M, T186P, T186R, T186S, T186Y, D202N, D202N/G333N, S210I, T215S/N218Y, N218Y, N218Y/R361T, N218Y/R361T/L398F, N218Y/L398F, W246Y, A254T/L398F, A271N, A271N/G333N, A271N/G333N/M390N/Q391N, A271N/G333N/Q391N, A271N/F352N/Q391N, S273L, Q275A, Q275G, K277Q, K277V, A278N, A278R, A278S, L280G, Q281I, Q281M, K283L, K283P, K283T, K283V, D284A, D284E, D284G, D284L, D284M, D284R, D284S, A287R, L300F, G303A, G303C, G303W, D304T, D304V, D304W, R325A, P331M, R332G, R332H, G333N/F352N, G333N/M390N/Q391N, G333N/M390S/Q391N, G333N/Q391N, Y334C, Y334V, T335A, T335L, I336F, I336G, I336S, I336T, V338L, A339G, A339N, A339Q, A339V, S340H, S340I, S340K, S340M, S340P, S340W, L341F, L341M, K343L, K343R, K343S, K343W, V359F, V359L, V359R, K360H, K360V, R361T, R361V, K362H, E367A, E367D, E367L, E367M, T369D, R371G, R373L, R373S, H375L, H375Q, N377Q, V382I, V382I/L398F, Q385R, E387N/Q391N, M390S 및 L398F로부터 선택된 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열번호 704를 기준으로 넘버링된다.
본 발명은 또한 서열번호 374에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 이상의 서열 동일성을 가지는 폴리펩타이드 서열 또는 이의 기능적 단편을 포함하는 재조합 알파-갈락토시다제 A를 제공하고, 여기서, 상기 재조합 알파-갈락토시다제 A는 10, 39, 44, 47, 92, 166, 206, 217, 247, 261, 271, 302, 316, 322, 337, 368 및 392로부터 선택된 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열번호 374를 기준으로 넘버링된다. 일부 실시양태에서, 재조합 알파-갈락토시다제 A는 서열번호 374에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 이상의 서열 동일성을 가지는 폴리펩타이드 서열 또는 이의 기능적 단편을 포함하고, 여기서, 상기 재조합 알파-갈락토시다제 A는 10A, 10C, 10D, 10E, 10F, 10G, 10H, 10I, 10K, 10L, 10M, 10N, 10Q, 10R, 10S, 10T, 10V, 10W, 10Y, 39A, 39C, 39D, 39F, 39G, 39H, 39I, 39K, 39L, 39M, 39N, 39P, 39Q, 39R, 39S, 39T, 39V, 39W, 39Y, 44A, 44C, 44D, 44E, 44F, 44G, 44H, 44I, 44K, 44L, 44N, 44P, 44Q, 44S, 44T, 44V, 44W, 44Y, 47A, 47C, 47D, 47E, 47F, 47G, 47H, 47I, 47K, 47L, 47M, 47N, 47P, 47Q, 47R, 47S, 47V, 47W, 47Y, 92A, 92C, 92D, 92E, 92F, 92G, 92I, 92K, 92L, 92M, 92N, 92P, 92Q, 92R, 92S, 92T, 92V, 92W, 92Y, 166A, 166C, 166D, 166E, 166F, 166G, 166H, 166I, 166K, 166L, 166M, 166N, 166Q, 166R, 166S, 166T, 166V, 166W, 166Y, 206C, 206D, 206E, 206F, 206G, 206H, 206I, 206K, 206L, 206M, 206N, 206P, 206Q, 206R, 206S, 206T, 206V, 206W, 206Y, 217A, 217C, 217D, 217E, 217F, 217G, 217H, 217I, 217K, 217L, 217M, 217N, 217P, 217Q, 217S, 217T, 217V, 217W, 217Y, 247A, 247C, 247E, 247F, 247G, 247H, 247I, 247K, 247L, 247M, 247N, 247P, 247Q, 247R, 247S, 247T, 247V, 247W, 247Y, 261A, 261C, 261D, 261E, 261F, 261H, 261I, 261K, 261L, 261M, 261N, 261P, 261Q, 261R, 261S, 261T, 261V, 261W, 261Y, 271C, 271D, 271E, 271F, 271G, 271H, 271I, 271K, 271L, 271M, 271N, 271P, 271Q, 271R, 271S, 271T, 271V, 271W, 271Y, 302A, 302C, 302D, 302E, 302F, 302G, 302H, 302I, 302L, 302M, 302N, 302P, 302Q, 302R, 302S, 302T, 302V, 302W, 302Y, 316A, 316C, 316E, 316F, 316G, 316H, 316I, 316K, 316L, 316M, 316N, 316P, 316Q, 316R, 316S, 316T, 316V, 316W, 316Y, 322A, 322C, 322D, 322E, 322F, 322G, 322H, 322K, 322L, 322M, 322N, 322P, 322Q, 322R, 322S, 322T, 322V, 322W, 322Y, 337A, 337C, 337D, 337E, 337F, 337G, 337H, 337I, 337K, 337L, 337M, 337N, 337Q, 337R, 337S, 337T, 337V, 337W, 337Y, 368C, 368D, 368E, 368F, 368G, 368H, 368I, 368K, 368L, 368M, 368N, 368P, 368Q, 368R, 368S, 368T, 368V, 368W, 368Y, 392A, 392C, 392D, 392E, 392F, 392G, 392H, 392I, 392K, 392L, 392M, 392N, 392P, 392Q, 392R, 392S, 392V, 392W 및 392Y로부터 선택된 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열번호 374를 기준으로 넘버링된다. 일부 실시양태에서, 재조합 알파-갈락토시다제 A는 서열번호 374에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 이상의 서열 동일성을 가지는 폴리펩타이드 서열 또는 이의 기능적 단편을 포함하고, 여기서, 상기 재조합 알파-갈락토시다제 A는 P10A, P10C, P10D, P10E, P10F, P10G, P10H, P10I, P10K, P10L, P10M, P10N, P10Q, P10R, P10S, P10T, P10V, P10W, P10Y, E39A, E39C, E39D, E39F, E39G, E39H, E39I, E39K, E39L, E39M, E39N, E39P, E39Q, E39R, E39S, E39T, E39V, E39W, E39Y, R44A, R44C, R44D, R44E, R44F, R44G, R44H, R44I, R44K, R44L, R44N, R44P, R44Q, R44S, R44T, R44V, R44W, R44Y, T47A, T47C, T47D, T47E, T47F, T47G, T47H, T47I, T47K, T47L, T47M, T47N, T47P, T47Q, T47R, T47S, T47V, T47W, T47Y, H92A, H92C, H92D, H92E, H92F, H92G, H92I, H92K, H92L, H92M, H92N, H92P, H92Q, H92R, H92S, H92T, H92V, H92W, H92Y, P166A, P166C, P166D, P166E, P166F, P166G, P166H, P166I, P166K, P166L, P166M, P166N, P166Q, P166R, P166S, P166T, P166V, P166W, P166Y, A206C, A206D, A206E, A206F, A206G, A206H, A206I, A206K, A206L, A206M, A206N, A206P, A206Q, A206R, A206S, A206T, A206V, A206W, A206Y, R217A, R217C, R217D, R217E, R217F, R217G, R217H, R217I, R217K, R217L, R217M, R217N, R217P, R217Q, R217S, R217T, R217V, R217W, R217Y, D247A, D247C, D247E, D247F, D247G, D247H, D247I, D247K, D247L, D247M, D247N, D247P, D247Q, D247R, D247S, D247T, D247V, D247W, D247Y, G261A, G261C, G261D, G261E, G261F, G261H, G261I, G261K, G261L, G261M, G261N, G261P, G261Q, G261R, G261S, G261T, G261V, G261W, G261Y, A271C, A271D, A271E, A271F, A271G, A271H, A271I, A271K, A271L, A271M, A271N, A271P, A271Q, A271R, A271S, A271T, A271V, A271W, A271Y, K302A, K302C, K302D, K302E, K302F, K302G, K302H, K302I, K302L, K302M, K302N, K302P, K302Q, K302R, K302S, K302T, K302V, K302W, K302Y, D316A, D316C, D316E, D316F, D316G, D316H, D316I, D316K, D316L, D316M, D316N, D316P, D316Q, D316R, D316S, D316T, D316V, D316W, D316Y, I322A, I322C, I322D, I322E, I322F, I322G, I322H, I322K, I322L, I322M, I322N, I322P, I322Q, I322R, I322S, I322T, I322V, I322W, I322Y, P337A, P337C, P337D, P337E, P337F, P337G, P337H, P337I, P337K, P337L, P337M, P337N, P337Q, P337R, P337S, P337T, P337V, P337W, P337Y, A368C, A368D, A368E, A368F, A368G, A368H, A368I, A368K, A368L, A368M, A368N, A368P, A368Q, A368R, A368S, A368T, A368V, A368W, A368Y, T392A, T392C, T392D, T392E, T392F, T392G, T392H, T392I, T392K, T392L, T392M, T392N, T392P, T392Q, T392R, T392S, T392V, T392W 및 T392Y로부터 선택된 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열번호 374를 기준으로 넘버링된다.
본 발명은 또한 서열번호 1022에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 이상의 서열 동일성을 가지는 폴리펩타이드 서열 또는 이의 기능적 단편을 포함하는 재조합 알파-갈락토시다제 A를 제공하고, 여기서, 상기 재조합 알파-갈락토시다제 A는 10, 10/392, 31, 31/39/44/166/302, 31/47, 31/283/284, 39, 39/44, 39/44/47, 39/44/47/261/283/284, 39/44/283, 39/44/339, 39/47/261, 39/92, 39/206, 39/284, 44, 44/284/302, 84, 84/92, 84/284/302/392, 84/316, 84/368/392, 92, 92/206/217, 92/206/275, 92/206/284, 92/206/302/368, 92/271, 92/271/277, 92/275/284, 92/283, 92/283/392, 92/284, 92/302, 92/316, 92/368, 155, 155/217, 155/368, 166, 166/283/284, 166/302, 206, 206/217, 206/334, 261, 261/283, 271, 271/368, 275, 283, 283/284, 283/392, 284, 302, 316, 334, 339, 368, 368/392 및 392로부터 선택된 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열번호 1022를 기준으로 넘버링된다. 일부 실시양태에서, 재조합 알파-갈락토시다제 A는 서열번호 1022에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 이상의 서열 동일성을 가지는 폴리펩타이드 서열 또는 이의 기능적 단편을 포함하고, 여기서, 상기 재조합 알파-갈락토시다제 A는 P10G, P10G/T392D, S31T, S31T/E39V/R44V/P166D/K302Y, S31T/T47R, S31T/K283L/D284A, E39L, E39L/H92V, E39L/A206E, E39L/D284S, E39V/R44V, E39V/R44V/T47R, E39V/R44V/T47R/G261S/K283L/D284A, E39V/R44V/K283T, E39V/R44V/A339N, E39V/T47R/G261S, R44V, R44V/D284E/K302Y, H84K, H84K/H92V, H84K/D284S/K302L/T392A, H84K/D316H, H84K/A368E/T392A, H92Q, H92T, H92T/A206E/R217N, H92T/A206E/K302T/A368E, H92T/A271K, H92T/A271K/K277R, H92T/K283P, H92T/K283V/T392W, H92T/D284M, H92T/K302L, H92T/A368E, H92V, H92V/A206E/D284S, H92V/A206Y/Q275A, H92V/Q275A/D284S, H92V/D284S, H92V/K302L, H92V/D316H, H155F, H155F/R217I, H155F/A368E, P166D, P166D/K283L/D284A, P166D/K302Y, A206E, A206E/R217N, A206I, A206Q, A206T/Y334C, A206Y, G261S, G261S/K283L, A271K, A271K/A368E, Q275A, K283L, K283P/T392W, K283T, K283T/D284E, D284E, D284M, D284S, K302L, K302Y, D316H, Y334C, A339N, A368E, A368E/T392W, T392A, T392D 및 T392W로부터 선택된 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열번호 1022를 기준으로 넘버링된다. 일부 실시양태에서, 재조합 알파-갈락토시다제 A는 서열번호 1022에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 이상의 서열 동일성을 가지는 폴리펩타이드 서열 또는 이의 기능적 단편을 포함하고, 여기서, 상기 재조합 알파-갈락토시다제 A는 10G, 10G/392D, 31T, 31T/39V/44V/166D/302Y, 31T/47R, 31T/283L/284A, 39L, 39L/92V, 39L/206E, 39L/284S, 39V/44V, 39V/44V/47R, 39V/44V/47R/261S/283L/284A, 39V/44V/283T, 39V/44V/339N, 39V/47R/261S, 44V, 44V/284E/302Y, 84K, 84K/92V, 84K/284S/302L/392A, 84K/316H, 84K/368E/392A, 92Q, 92T, 92T/206E/217N, 92T/206E/302T/368E, 92T/271K, 92T/271K/277R, 92T/283P, 92T/283V/392W, 92T/284M, 92T/302L, 92T/368E, 92V, 92V/206E/284S, 92V/206Y/275A, 92V/275A/284S, 92V/284S, 92V/302L, 92V/316H, 155F, 155F/217I, 155F/368E, 166D, 166D/283L/284A, 166D/302Y, 206E, 206E/217N, 206I, 206Q, 206T/334C, 206Y, 261S, 261S/283L, 271K, 271K/368E, 275A, 283L, 283P/392W, 283T, 283T/284E, 284E, 284M, 284S, 302L, 302Y, 316H, 334C, 339N, 368E, 368E/392W, 392A, 392D 및 392W로부터 선택된 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열번호 1022를 기준으로 넘버링된다.
일부 실시양태에서, 본 발명의 알파-갈락토시다제 A는 표 2-1, 5-1, 6-1, 7-1, 8-1, 9-1, 11-1, 12-1, 및/또는 13-1에 제공된 바와 같은 적어도 하나의 위치에서 적어도 하나의 돌연변이를 포함하고, 여기서, 상기 위치는 서열번호 2 또는 표에 나타낸 바와 같은 또 다른 기준 서열을 기준으로 넘버링된다. 일부 추가 실시양태에서, 재조합 알파-갈락토시다제 A는 인간 알파-갈락토시다제 A로부터 유도된다. 일부 추가 실시양태에서, 재조합 알파-갈락토시다제 A는 서열번호 8, 58, 158, 372, 374, 704 및/또는 1022의 폴리펩타이드 서열을 포함한다.
일부 실시양태에서, 재조합 알파-갈락토시다제 A는 서열번호 2, 8, 58, 158, 372, 374, 704 및/또는 1022의 알파-갈락토시다제 A보다 더 열안정하다. 일부 추가 실시양태에서, 재조합 알파-갈락토시다제 A는 서열번호 2, 8, 58, 158, 372, 374, 704 및/또는 1022의 알파-갈락토시다제 A보다 pH 7에서 더 안정하다. 또 다른 일부 추가의 실시양태에서, 재조합 알파-갈락토시다제 A는 서열번호 2, 8, 58, 158, 372, 374, 704 및/또는 1022의 알파-갈락토시다제 A보다 pH 4에서 더 안정하다. 또 다른 일부 추가의 실시양태에서, 재조합 알파-갈락토시다제 A는 서열번호 2, 8, 58, 158, 372, 374, 704 및/또는 1022의 알파-갈락토시다제 A보다 pH 7에서 더 안정하고 pH 4에서 더 안정하다. 또 다른 추가의 실시양태에서, 재조합 알파-갈락토시다제 A는 서열번호 2, 8, 58, 158, 372, 374, 704, 및/또는 1022의 알파-갈락토시다제 A보다 혈청에 대한 노출에 더 안정하다. 일부 추가의 실시양태에서, 재조합 알파-갈락토시다제 A는 서열번호 2, 8, 58, 158, 372, 374, 704 및/또는 1022의 알파-갈락토시다제 A보다 라이소좀에서 더 안정하다. 또 다른 일부 추가의 실시양태에서, 재조합 알파-갈락토시다제 A는 서열번호 2, 8, 58, 158, 372, 374, 704 및/또는 1022의 알파-갈락토시다제 A보다 세포에 의해 더 용이하게 흡수된다. 일부 추가의 실시양태에서, 재조합 알파-갈락토시다제 A는 서열번호 2, 8, 58, 158, 372, 374, 704 및/또는 1022의 알파-갈락토시다제 A보다 세포로부터 더 많은 글로보트리아오실세라마이드를 고갈시킨다. 또 다른 일부 추가의 실시양태에서, 재조합 알파-갈락토시다제 A는 서열번호 2, 8, 58, 158, 372, 374, 704 및/또는 1022의 알파-갈락토시다제 A에 비해 세포 내로의 개선된 흡수를 나타낸다. 일부 추가의 실시양태에서, 재조합 알파-갈락토시다제 A는 서열번호 2, 8, 58, 158, 372, 374, 704 및/또는 1022의 알파-갈락토시다제 A보다 더 적은 면역원성을 나타낸다. 일부 추가의 실시양태에서, 재조합 알파-갈락토시다제 A는 기준 서열에 비해, i) 향상된 촉매 활성; ii) pH 7에 대한 증가된 내성; iii) pH 4에 대한 증가된 내성; iv) 혈청에 대한 증가된 내성; v) 세포 내로의 개선된 흡수; vi) 감소된 면역원성; 또는 vii) 세포로부터의 글로보트리아오실세라마이드의 증가된 고갈; 또는 i), ii), iii), iv), v), vi) 또는 vii) 중 임의의 성질의 조합으로부터 선택된 적어도 하나의 개선된 성질을 나타낸다. 일부 실시양태에서, 기준 서열은 서열번호 2, 8, 58, 158, 372, 374, 704 및/또는 1022이다. 일부 추가 실시양태에서, 재조합 알파-갈락토시다제 A는 정제된다.
본 발명은 또한 본원(예를 들어, 표 2-1, 5-1, 6-1, 7-1, 8-1, 9-1, 11-1, 12-1 및/또는 13-1)에 제공된 바와 같은 적어도 하나의 재조합 알파-갈락토시다제 A를 코딩하는 재조합 폴리뉴클레오타이드 서열도 제공한다. 일부 실시양태에서, 상기 폴리뉴클레오타이드 서열은 DNA, RNA 및 mRNA로부터 선택된다. 일부 실시양태에서, 재조합 폴리뉴클레오타이드 서열은 코돈 최적화된다.
본 발명은 또한 본원(예를 들어, 표 2-1, 5-1, 6-1, 7-1, 8-1, 9-1, 11-1, 12-1 및/또는 13-1)에 제공된 바와 같은 적어도 하나의 재조합 알파-갈락토시다제 A를 코딩하는 재조합 폴리뉴클레오타이드 서열을 포함하는 발현 벡터도 제공한다. 일부 실시양태에서, 재조합 폴리뉴클레오타이드 서열은 조절 서열에 작동 가능하게 연결된다. 일부 추가 실시양태에서, 조절 서열은 프로모터이다. 일부 추가 실시양태에서, 프로모터는 이종 프로모터이다. 일부 실시양태에서, 상기 발현 벡터는 본원에 제공된 바와 같은 신호 서열을 추가로 포함한다.
본 발명은 또한 본원에 제공된 바와 같은 적어도 하나의 발현 벡터를 포함하는 숙주 세포도 제공한다. 일부 실시양태에서, 상기 숙주 세포는 본원(예를 들어, 표 2-1, 5-1, 6-1, 7-1, 8-1, 9-1, 11-1, 12-1 및/또는 13-1)에 제공된 바와 같은 적어도 하나의 재조합 알파-갈락토시다제 A를 코딩하는 재조합 폴리뉴클레오타이드 서열을 포함하는 발현 벡터를 포함한다. 일부 실시양태에서, 상기 숙주 세포는 진핵세포 및 원핵세포로부터 선택된다. 일부 실시양태에서, 상기 숙주 세포는 진핵 세포이다. 일부 추가 실시양태에서, 상기 숙주 세포는 포유류 세포이다.
본 발명은 또한 알파-갈락토시다제 A 변이체의 제조 방법으로서, 재조합 폴리뉴클레오타이드에 의해 코딩된 알파-갈락토시다제 A가 생성되는 조건 하에서 본원에 제공된 숙주 세포를 배양하는 단계를 포함하는, 방법도 제공한다. 일부 실시양태에서, 상기 방법은 알파-갈락토시다제 A를 회수하는 단계도 포함한다. 일부 추가 실시양태에서, 상기 방법은 알파-갈락토시다제 A를 정제하는 단계를 추가로 포함한다. 본 발명은 또한 본원(예를 들어, 표 2-1, 5-1, 6-1, 7-1, 8-1, 9-1, 11-1, 12-1 및/또는 13-1)에 제공된 바와 같은 적어도 하나의 재조합 알파-갈락토시다제 A를 포함하는 조성물도 제공한다. 일부 실시양태에서, 본 발명은 약학 조성물을 제공한다. 일부 실시양태에서, 본 발명은 또한 본원에 제공된 적어도 하나의 재조합 폴리뉴클레오타이드를 포함하는 약학 조성물을 제공한다. 일부 추가 실시양태에서, 본 발명은 본원에 제공된 효소 조성물을 포함하는, 파브리병의 치료를 위한 약학 조성물을 제공한다. 일부 실시양태에서, 상기 약학 조성물은 약학적으로 허용 가능한 담체 및/또는 부형제를 추가로 포함한다. 일부 추가 실시양태에서, 상기 약학 조성물은 인간에게 비경구적으로 주사하거나 주입하기에 적합하다.
본 발명은 또한 대상체에서 파브리병의 증상을 치료하고/하거나 예방하는 방법으로서, 파브리병을 가진 대상체를 제공하는 단계, 본원(예를 들어, 표 2-1, 5-1, 6-1, 7-1, 8-1, 9-1, 11-1, 12-1 및/또는 13-1)에 제공된 바와 같은 적어도 하나의 재조합 알파-갈락토시다제 A를 포함하는 적어도 하나의 약학 조성물을 제공하는 단계, 및 상기 약학 조성물을 상기 대상체에게 투여하는 단계를 포함하는, 방법도 제공한다. 일부 실시양태에서, 파브리병의 증상은 상기 대상체에서 호전된다. 일부 추가 실시양태에서, 본 발명의 약학 조성물이 투여되는 대상체는 파브리병의 증상을 나타내는 대상체에 의해 요구된 식이보다 그의 지방 함량이 덜 제한된 식이를 먹을 수 있다. 일부 실시양태에서, 대상체는 유아 또는 소아이지만, 일부 대안적 실시양태에서 대상체는 성인 또는 청년이다.
본 발명은 또한 본원에 제공된 조성물의 용도를 제공한다.
도 1은 30℃ 내지 50℃의 온도에서 1시간 동안 인큐베이션한 후 GLA 변이체의 상대적인 활성을 보여주는 그래프를 제공한다.
도 2는 0시간 내지 24시간 동안 37℃에서 인간 혈청으로 챌린지한 후, GLA 변이체의 상대적인 활성을 보여주는 그래프를 제공한다.
도 3은 0시간 내지 24시간 동안 37℃에서 인간 라이소좀 추출물로 챌린지한 후, GLA 변이체의 상대적인 활성을 보여주는 그래프를 제공한다.
도 4는 배양된 파브리 환자 섬유모세포와 함께 37℃에서 4시간 동안 인큐베이션한 후 야생형에 비해 상대적인 활성으로서 표현된, 상이한 정제된 GLA 변이체들의 세포 흡수를 보여주는 그래프를 제공한다.
도 5는 투여한 지 1주, 2주 및 4주 후 서열번호 2에 비해 파브리 마우스의 심장에서 GLA 변이체의 활성을 보여주는 그래프를 제공한다.
도 6은 투여한 지 1주 및 2주 후 GLA 변이체로 처리된 파브리 마우스의 심장에서 잔류 Gb3을 보여주는 그래프를 제공한다.
도 7은 0시간 내지 24시간 동안 인간 혈청으로 챌린지 후 GLA 변이체의 잔류 활성을 보여주는 그래프를 제공한다.
도 8은 37℃에서 4시간 인큐베이션 및 3일 세척 후 배양된 파브리 환자 섬유모세포에서 정제된 GLA 변이체의 세포 흡수를 보여주는 그래프를 제공한다.
도 9는 마지막 처리 7일 후 파브리 마우스 모델의 심장에서 생체내 효소 활성을 보여주는 그래프를 제공한다.
도 10은 마지막 처리 7일 후 파브리 마우스 모델의 신장에서 생체내 효소 활성을 보여주는 그래프를 제공한다.
도 11은 심장 조직에서 Gb3 분해를 보여주는 그래프를 제공한다.
도 12는 신장 조직에서 Gb3 분해를 보여주는 그래프를 제공한다.
도 13은 심장 조직에서 lyso-Gb3 분해를 보여주는 그래프를 제공한다.
도 14는 신장 조직에서 lyso-Gb3 분해를 보여주는 그래프를 제공한다.
도 2는 0시간 내지 24시간 동안 37℃에서 인간 혈청으로 챌린지한 후, GLA 변이체의 상대적인 활성을 보여주는 그래프를 제공한다.
도 3은 0시간 내지 24시간 동안 37℃에서 인간 라이소좀 추출물로 챌린지한 후, GLA 변이체의 상대적인 활성을 보여주는 그래프를 제공한다.
도 4는 배양된 파브리 환자 섬유모세포와 함께 37℃에서 4시간 동안 인큐베이션한 후 야생형에 비해 상대적인 활성으로서 표현된, 상이한 정제된 GLA 변이체들의 세포 흡수를 보여주는 그래프를 제공한다.
도 5는 투여한 지 1주, 2주 및 4주 후 서열번호 2에 비해 파브리 마우스의 심장에서 GLA 변이체의 활성을 보여주는 그래프를 제공한다.
도 6은 투여한 지 1주 및 2주 후 GLA 변이체로 처리된 파브리 마우스의 심장에서 잔류 Gb3을 보여주는 그래프를 제공한다.
도 7은 0시간 내지 24시간 동안 인간 혈청으로 챌린지 후 GLA 변이체의 잔류 활성을 보여주는 그래프를 제공한다.
도 8은 37℃에서 4시간 인큐베이션 및 3일 세척 후 배양된 파브리 환자 섬유모세포에서 정제된 GLA 변이체의 세포 흡수를 보여주는 그래프를 제공한다.
도 9는 마지막 처리 7일 후 파브리 마우스 모델의 심장에서 생체내 효소 활성을 보여주는 그래프를 제공한다.
도 10은 마지막 처리 7일 후 파브리 마우스 모델의 신장에서 생체내 효소 활성을 보여주는 그래프를 제공한다.
도 11은 심장 조직에서 Gb3 분해를 보여주는 그래프를 제공한다.
도 12는 신장 조직에서 Gb3 분해를 보여주는 그래프를 제공한다.
도 13은 심장 조직에서 lyso-Gb3 분해를 보여주는 그래프를 제공한다.
도 14는 신장 조직에서 lyso-Gb3 분해를 보여주는 그래프를 제공한다.
본 발명은 조작된 인간 알파-갈락토시다제 폴리펩타이드 및 이의 조성물을 제공한다. 조작된 인간 알파-갈락토시다제 폴리펩타이드는 개선된 열안정성, 혈청 안정성, 개선된 세포 흡수, 및 산성(pH <4) 조건과 염기성(pH >7) 조건의 양 조건 하에서의 안정성, 감소된 면역원성, 및 세포로부터의 개선된 글로보트리아오실세라마이드 제거를 제공하도록 최적화되었다. 또한, 본 발명은 치료 목적을 위한 조작된 인간 알파-갈락토시다제 폴리펩타이드를 포함하는 조성물의 용도에 관한 것이다. 일부 실시양태에서, 조작된 인간 알파-갈락토시다제 폴리펩타이드는 안정성을 유지하면서 개선된 세포 흡수를 제공하도록 최적화되었다. 본 발명은 또한 치료 목적을 위한 조작된 인간 알파-갈락토시다제 폴리펩타이드를 포함하는 조성물의 용도에 관한 것이기도 하다.
일부 경우, 파브리병의 치료를 위한 효소 대체 요법(예를 들어, FABRAZYME®아갈시다제(agalsidase) 베타; Genzyme)은 적격 개체의 경우 고려된다. 현재 사용되는 효소 대체 요법은 야생형 인간 GLA의 재조합적으로 발현된 형태이다. 정맥내로 투여된 GLA가 순환하고, 수용체, 주로 만노스 6-포스페이트 수용체(M6PR) 매개 세포내이입을 통해 세포 내로 흡수되고, 표적 장기의 엔도좀/라이소좀으로 이동하고 여기서, 축적된 Gb3을 제거하는 것으로 공지되어 있다. 이 약물은 신경병증성 통증 및 일시적인 허혈성 발작이 감소된 비율로 계속 일어나기 때문에 환자 증상을 완전히 완화하지 못한다. 추가로, 대다수의 표적 장기들에 의한 GLA의 흡수는 혈관이 잘 형성되고 M6PR이 풍부한 간에 비해 약하고, 상기 효소는 혈액 및 라이소좀의 pH에서 불안정하다. 따라서, 이용 가능한 치료에 대한 문제가 남아있다. 또한, 환자는 면역 반응(투여된 약물을 표적화하는 IgG 및 IgE 항체)을 발생시킬 수 있고, 심각한 알레르기(아나필락시스) 반응 및 심각한 주입 반응을 겪을 수 있고 심지어 사망할 수 있다. 본 발명은 현재 이용 가능한 치료에 비해, 부작용이 감소되고 결과가 개선된, 파브리병의 치료에 적합한 더 안정하고 효과적인 효소를 제공하기 위한 것이다. 실제로, 본 발명은 효소가 혈류 내로 도입될 때 접하는 혈액(pH 7.4)에서 증가된 안정성을 가진 재조합 GLA 효소를 제공하기 위한 것이다. 추가로, 상기 효소는 이 효소가 치료 동안 활성인 위치인 라이소좀의 pH(pH 4.3)에서 증가된 안정성을 가진다. 따라서, 다양한 효소 변이체 라이브러리들의 고처리율 스크리닝을 이용하는, 인간 HEK293T 세포에서 재조합적으로 발현된 인간 GLA의 유도진화를 이용함으로써, 유지된 안정성, 개선된 글로보트리아오실세라마이드 제거 및 세포 흡수를 가진 신규 GLA 변이체를 제공하였다. 일부 실시양태에서, GLA 변이체는 감소된 면역원성을 나타낸다.
약어 및 정의
달리 정의되어 있지 않은 한, 본원에서 사용된 모든 기술 용어들 및 과학 용어들은 일반적으로 본 발명이 속하는 분야에서 통상의 기술을 가진 자에 의해 통상적으로 이해되는 의미와 동일한 의미를 가진다. 일반적으로, 본원에서 사용된 명명법, 및 이하에 기재된 세포 배양, 분자유전학, 미생물학, 생화학, 유기 화학, 분석 화학 및 핵산 화학의 실험실 절차는 잘 공지되어 있고 당분야에서 통상적으로 이용된다. 이러한 기법은 잘 공지되어 있고, 당분야에서 숙련된 자에게 잘 공지된 다수의 교재들 및 참고문헌들에 기재되어 있다. 표준 기법 및 이의 변형은 화학적 합성 및 화학적 분석을 위해 이용된다. 본원에서 언급된 상기 및 하기 모든 특허들, 특허 출원들, 논문들 및 간행물들은 명백히 본원에 참고로 포함된다.
본원에 기재된 방법 및 재료와 유사하거나 동등한 임의의 적합한 방법 및 재료가 본 발명의 실시에서 사용이 확인되지만, 일부 방법들 및 재료들이 본원에 기재된다. 방법, 프로토콜 및 시약은 당분야에서 숙련된 자에 의해 이용되는 상황에 따라 달라질 수 있기 때문에, 본 발명은 기재된 특정 방법, 프로토콜 및 시약으로 제한되지 않음을 이해해야 한다. 따라서, 바로 아래에 정의된 용어들은 전체로서 본 출원을 참고함으로써 더 완전히 기재된다. 본원에서 언급된 상기 및 하기 모든 특허들, 특허 출원들, 논문들 및 간행물들은 명백히 본원에 참고로 포함된다.
또한, 본원에서 사용된 바와 같이, 문맥이 달리 분명히 표시하지 않은 한, 단수는 복수의 대상을 포함한다.
수치 범위는 범위를 한정하는 숫자를 포함한다. 따라서, 본원에 개시된 모든 수치 범위는 이러한 더 넓은 수치 범위 내에 속하는 모든 더 좁은 수치 범위가 본원에 모두 명확히 기재된 것처럼 모든 이러한 더 작은 수치 범위를 포괄하기 위한 것이다. 본원에 개시된 모든 최대(또는 최소) 수치 제한은 더 낮은(또는 더 높은) 수치 제한이 본원에 명확히 기재된 것처럼 모든 이러한 더 낮은(또는 더 높은) 수치 제한도 포함하기 위한 것이다.
용어 "약"은 특정 값에 대한 허용 가능한 오차를 의미한다. 일부 경우, "약"은 주어진 값 범위의 0.05%, 0.5%, 1.0% 또는 2.0% 이내에 있음을 의미한다. 일부 경우, "약"은 주어진 값의 1, 2, 3 또는 4 표준 편차 이내에 있음을 의미한다.
나아가, 본원에 제공된 표제는 전체로서 본 출원을 참고함으로써 가질 수 있는 발명의 다양한 양태 또는 실시양태의 제한이 아니다. 따라서, 바로 아래에 정의된 용어들은 전체로서 본 출원을 참고함으로써 더 완전히 정의된다. 그럼에도 불구하고, 발명의 이해를 용이하게 하기 위해, 다수의 용어들이 이하에 정의된다.
달리 표시되어 있지 않은 한, 핵산은 5'에서 3' 방향으로 좌측에서 우측으로 작성되고; 아미노산 서열은 아미노에서 카르복시 방향으로 좌측에서 우측으로 각각 작성된다.
본원에서 사용된 바와 같이, 용어 "포함하는" 및 이의 동족어는 이들의 포괄적인 의미로 사용된다(즉, 용어 "포괄하는" 및 이의 상응하는 동족어와 동등함).
본원에서 사용된 바와 같이, "EC"는 국제 생화학 및 분자생물학 연합의 명명위원회(NC-IUBMB)의 효소 명명법을 지칭한다. IUBMB 생화학적 분류는 효소가 촉매로서 작용하는 화학적 반응에 기반한, 효소에 대한 수치 분류 시스템이다.
본원에서 사용된 바와 같이, "ATCC"는 유전자 및 균주를 포함하는 생물저장소 수집물을 가진 어메리칸 타입 컬쳐 콜렉션(American Type Culture Collection)을 지칭한다.
본원에서 사용된 바와 같이, "NCBI"는 국립 생물학적 정보 센터 및 이에 의해 제공된 서열 데이터베이스를 지칭한다.
"단백질", "폴리펩타이드" 및 "펩타이드"는 길이 또는 번역 후 변형(예를 들어, 글리코실화 또는 인산화)와 관계없이 아미드 결합에 의해 공유 결합된 적어도 2개의 아미노산들의 중합체를 표시하기 위해 본원에서 교환 가능하게 사용된다.
"아미노산"은 본원에서 그의 통상적으로 공지된 3-문자 부호, 또는 IUPAC-IUB 생화학 명명 위원회에 의해 권장된 1-문자 부호에 의해 지칭된다. 마찬가지로, 뉴클레오타이드는 그의 통상적으로 허용되는 단일 문자 코드에 의해 지칭될 수 있다. 유전적으로 코딩된 아미노산에 사용되는 약어는 통상적이며 다음과 같다: 알라닌(Ala 또는 A), 아르기닌(Arg 또는 R), 아스파라긴(Asn 또는 N), 아스파르테이트(Asp 또는 D), 시스테인(Cys 또는 C), 글루타메이트(Glu 또는 E), 글루타민(Gln 또는 Q), 히스티딘(His 또는 H), 이소류신(Ile 또는 I), 류신(Leu 또는 L), 라이신(Lys 또는 K), 메티오닌(Met 또는 M), 페닐알라닌(Phe 또는 F), 프롤린(Pro 또는 P), 세린(Ser 또는 S), 트레오닌(Thr 또는 T), 트립토판(Trp 또는 W), 티로신(Tyr 또는 Y) 및 발린(Val 또는 V). 3-문자 약어가 사용되는 경우 "L" 또는 "D"가 앞에 오지 않거나 이러한 약어가 사용된 문맥에서 명확하지 않는 한, 아미노산은 α-탄소(Cα)에 대해 L- 또는 D-구성일 수 있다. 예를 들어, "Ala"는 α-탄소에 대한 배열을 지정하지 않고 알라닌을 지정하는 반면, "D-Ala" 및 "L-Ala"는 각각 D-알라닌 및 L-알라닌을 지정한다. 폴리펩타이드 서열이 여러 1-문자 또는 3-문자 약어(또는 이들의 혼합)로 제시되는 경우, 서열은 일반적인 관례에 따라 아미노(N)에서 카르복시(C) 방향으로 제시된다.
유전적으로 코딩하는 뉴클레오사이드에 사용되는 약어는 통상적이며 다음과 같다: 아데노신(A); 구아노신(G); 시티딘(C); 티미딘(T); 및 우리딘(U). 구체적으로 명시되지 않는 한, 약칭된 뉴클레오사이드는 리보뉴클레오사이드 또는 2'-데옥시리보뉴클레오사이드일 수 있다. 뉴클레오사이드는 개별적인 기준으로 또는 집합적인 기준으로 리보뉴클레오사이드 또는 2'-데옥시리보뉴클레오사이드인 것으로 명시될 수 있다. 핵산 서열이 여러 1-문자 약어로 제시되는 경우, 서열은 일반적인 관례에 따라 5'에서 3' 방향으로 제시되며, 포스페이트는 표시되지 않는다.
"조작된," "재조합," "비자연적으로 발생된," 및 "변이체"라는 용어는 세포, 폴리뉴클레오타이드 또는 폴리펩타이드를 참조로 하여 사용될 때, 물질, 또는 자연상에는 달리 존재하지 않는 방식으로 변형된, 또는 그와 동일하지만, 합성 물질로부터 및/또는 재조합 기법을 사용하는 조작에 의해 제조 또는 유래된 상기 물질의 자연적 또는 천연 형태에 상응하는 물질을 지칭한다.
본원에서 사용된 바와 같이, "UTR"은 mRNA 폴리뉴클레오타이드의 비번역 영역을 지칭한다. 일부 실시양태에서, "5' 비번역 영역" 또는 "5' UTR"은 "리더 서열" 또는 "리더 RNA"로서 지칭된다. 이 mRNA 영역은 시작 코돈으로부터 바로 업스트림에 있다. 일부 실시양태에서, "3' 비번역 영역" 또는 "3' UTR"은 정지 코돈으로부터 바로 다운스트림에 있는 mRNA 영역이다. 다양한 유기체들(예를 들어, 원핵생물, 진핵생물 및 바이러스)에서, 이 영역들 둘 다가 번역 조절 및 세포내 수송에 중요하다.
본원에서 사용된 바와 같이, "야생형", "WT", 및 "천연 생성"은 자연에서 발견되는 형태를 지칭한다. 예를 들어, 야생형 폴리펩타이드 또는 폴리뉴클레오타이드 서열은 자연에서 공급원으로부터 단리될 수 있고 인간 조작에 의해 의도적으로 변형되지 않은, 유기체에 존재하는 서열이다.
본원에서 사용된 바와 같이, "코딩 서열"은 단백질의 아미노산 서열을 코딩하는 핵산(예를 들어, 유전자)의 일부를 지칭한다.
용어 "퍼센트(%) 서열 동일성"은 폴리뉴클레오타이드들 및 폴리펩타이드들 사이의 비교를 나타내기 위해 본원에서 사용되고, 비교 윈도우에 걸쳐 2개의 최적으로 정렬된 서열들을 비교함으로써 측정되고, 여기서, 비교 윈도우에서 폴리뉴클레오타이드 또는 폴리펩타이드 서열의 일부는 2개의 서열들의 최적 정렬을 위해 기준 서열에 비해 추가 또는 결실(즉, 갭)을 포함할 수 있다. 백분율은 두 서열들에서 동일한 핵산 염기 또는 아미노산 잔기가 발생하는 위치의 수를 확인하여 일치된 위치의 수를 산출하고, 일치된 위치의 수를 비교 윈도우 내의 위치의 총수로 나누고, 결과에 100을 곱하여 서열 동일성의 백분율을 산출함으로써 계산될 수 있다. 대안적으로, 백분율은 두 서열들에서 동일한 핵산 염기 또는 아미노산 잔기가 발생하는 위치의 수, 또는 핵산 염기 또는 아미노산 잔기가 갭과 정렬되는 위치의 수를 확인하여 일치된 위치의 수를 산출하고, 일치된 위치의 수를 비교 윈도우 내의 위치의 총수로 나누고, 결과에 100을 곱하여 서열 동일성의 백분율을 산출함으로써 계산될 수 있다. 당분야에서 숙련된 자는 2개의 서열들을 정렬하는 데 이용될 수 있는 많은 확립된 알고리즘들이 있음을 인식한다. 비교를 위한 서열들의 최적 정렬은 당분야에서 공지되어 있는 바와 같이, 예를 들어, 스미쓰와 워터만(Smith and Waterman)의 국소 상동성 알고리즘(Smith and Waterman, Adv. Appl. Math., 2:482 [1981]), 니들만과 분슈(Needleman and Wunsch)의 상동성 정렬 알고리즘(Needleman and Wunsch, J. Mol. Biol., 48:443 [1970]), 피어슨과 립만(Pearson and Lipman)의 유사성 검색 방법(Pearson and Lipman, Proc. Natl. Acad. Sci. USA 85:2444 [1988]), 이 알고리즘들의 전산화된 실행(예를 들어, GCG 위스콘신 소프트웨어 팩키지 내의 GAP, BESTFIT, FASTA 및 TFASTA), 또는 육안 검사에 의해 수행될 수 있다. 퍼센트 서열 동일성 및 서열 유사성을 측정하는 데 적합한 알고리즘의 예는 알츠철 연구진(Altschul et al.)에 의해 기재된 BLAST 및 BLAST 2.0 알고리즘(각각 문헌[Altschul et al., J. Mol. Biol., 215: 403-410 [1990]]; 및 문헌[Altschul et al., 1977, Nucleic Acids Res., 3389-3402 [1977]] 참조)을 포함하나, 이들로 제한되지 않는다. BLAST 분석을 수행하기 위한 소프트웨어는 국립 생물공학 정보 센터 웹사이트를 통해 공개적으로 입수될 수 있다. 이 알고리즘은 먼저 데이터베이스 서열에서 동일한 길이의 단어와 정렬될 때 일부 양의 값 역치 점수 T와 일치하거나 이 점수를 충족시키는 길이 W의 짧은 단어를 질의 서열에서 확인함으로써 고득점 서열 쌍(HSP)을 확인하는 단계를 포함한다. T는 이웃 단어 점수 역치로서 지칭된다(상기 문헌[Altschul et al.] 참조). 이 초기 이웃 단어 히트(hit)는 이를 함유하는 더 긴 HSP를 찾는 검색을 시작하기 위한 시드(seed)로서 작용한다. 그 다음, 누적 정렬 점수가 증가될 수 있는 한, 단어 히트는 각각의 서열을 따라 양 방향으로 확장된다. 뉴클레오타이드 서열의 경우, 파라미터 M(한 쌍의 일치 잔기들에 대한 보상 점수; 항상 >0) 및 N(불일치 잔기들에 대한 벌점 점수; 항상 <0)을 사용하여 누적 점수를 계산한다. 아미노산 서열의 경우, 점수화 매트릭스를 이용하여 누적 점수를 계산한다. 누적 정렬 점수가 그의 최대 달성된 값으로부터 양 X만큼 떨어지거나; 누적 점수가 하나 이상의 음의 점수화 잔기 정렬의 축적으로 인해 0 이하로 내려가거나; 어느 한 서열의 말단에 도달될 때, 각 방향에서 단어 히트의 확장은 중단된다. BLAST 알고리즘 파라미터 W, T 및 X는 정렬의 민감성 및 속도를 결정한다. (뉴클레오타이드 서열의 경우) BLASTN 프로그램은 11의 단어길이(W), 10의 기댓값(E), M=5, N=-4, 및 두 가닥들의 비교를 디폴트로서 사용한다. 아미노산 서열의 경우, BLASTP 프로그램은 3의 단어길이(W), 10의 기댓값(E) 및 BLOSUM62 점수화 매트릭스를 디폴트로서 사용한다(문헌[Henikoff and Henikoff, Proc. Natl. Acad. Sci. USA 89:10915 [1989]] 참조). 서열 정렬 및 % 서열 동일성의 예시적인 측정은 제공된 디폴트 파라미터를 사용하는, GCG 위스콘신 소프트웨어 팩키지(Accelrys, 위스콘신주 매디슨 소재) 내의 BESTFIT 또는 GAP 프로그램을 이용할 수 있다.
본원에서 사용된 바와 같이, "기준 서열"은 서열 비교를 위한 기준으로서 사용되는 규정된 서열을 지칭한다. 기준 서열은 더 큰 서열의 서브 세트, 예를 들어, 전장 유전자 또는 폴리펩타이드 서열의 분절일 수 있다. 일반적으로, 기준 서열은 길이가 적어도 20개 뉴클레오타이드 또는 아미노산 잔기이거나, 길이가 적어도 25개 잔기이거나, 길이가 적어도 50개 잔기이거나, 길이가 적어도 100개 잔기이거나, 핵산 또는 폴리펩타이드의 전장이다. 2개의 폴리뉴클레오타이드들 또는 폴리펩타이드들이 각각 (1) 2개의 서열들 사이에 유사한 서열(즉, 완전한 서열의 일부)을 포함할 수 있고, (2) 2개의 서열들 사이에 상이한 서열을 추가로 포함할 수 있고, 2개(또는 그 이상)의 폴리뉴클레오타이드들 또는 폴리펩타이드들 사이의 서열 비교는 전형적으로 "비교 윈도우"에 걸쳐 2개의 폴리뉴클레오타이드들 또는 폴리펩타이드들의 서열을 비교하여, 서열이 유사한 국소 영역을 확인하고 비교함으로써 수행된다. 일부 실시양태에서, "기준 서열"은 일차 아미노산 서열에 기반할 수 있고, 여기서, 기준 서열은 일차 서열에서 하나 이상의 변화를 가질 수 있는 서열이다. "비교 윈도우"는 적어도 약 20개의 연속 뉴클레오타이드 위치들 또는 아미노산 잔기들의 개념적 분절을 지칭하고, 여기서, 서열은 적어도 20개의 연속 뉴클레오타이드들 또는 아미노산들의 기준 서열과 비교될 수 있고, 비교 윈도우 내의 서열의 일부는 2개의 서열들의 최적 정렬을 위해 (추가 또는 결실을 포함하지 않는) 기준 서열에 비해 20% 이하의 추가 또는 결실(즉, 갭)을 포함할 수 있다. 비교 윈도우는 20개의 연속 잔기들보다 더 길 수 있고, 임의적으로 30개, 40개, 50개, 100개 또는 더 긴 윈도우를 포함한다.
소정의 아미노산 또는 폴리뉴클레오타이드 서열을 넘버링하는 상황에서 사용될 때 "에 상응하는", "을(를) 기준으로" 또는 "과(와) 관련하여"는 소정의 아미노산 또는 폴리뉴클레오타이드 서열이 기준 서열과 비교될 때 특정된 기준 서열의 잔기의 넘버링을 지칭한다. 다시 말해, 소정의 중합체의 잔기 수 또는 잔기 위치는 소정의 아미노산 또는 폴리뉴클레오타이드 서열 내의 잔기의 실제 숫자 위치에 의해 표기되기보다는 오히려 기준 서열과 관련하여 표기된다. 예를 들어, 소정의 아미노산 서열, 예컨대, 조작된 GLA의 아미노산 서열은 갭을 도입하여 두 서열들 사이의 잔기 일치를 최적화함으로써 기준 서열에 정렬될 수 있다. 이 경우, 갭이 존재할지라도, 소정의 아미노산 또는 폴리뉴클레오타이드 서열에서 잔기는 이 서열에 정렬된 기준 서열과 관련하여 넘버링된다.
본원에서 사용된 바와 같이, "아미노산 차이" 또는 "잔기 차이"는 폴리펩타이드 서열의 한 위치에 있는 아미노산 잔기와 기준 서열의 상응하는 위치에 있는 아미노산 잔기의 차이를 지칭한다. 아미노산 차이의 위치는 일반적으로 본원에서 "Xn"으로서 지칭되고, 여기서, n은 잔기 차이의 기준이 되는 기준 서열 내의 상응하는 위치를 지칭한다. 예를 들어, "서열번호 8에 비해 위치 X44의 잔기 차이"는 서열번호 8의 위치 44에 상응하는 폴리펩타이드 위치에서 아미노산 잔기의 차이를 지칭한다. 따라서, 서열번호 8의 기준 폴리펩타이드가 위치 44에서 아르기닌을 가진 경우, "서열번호 8에 비해 위치 44에서 잔기 차이"는 서열번호 8의 위치 44에 상응하는 폴리펩타이드의 위치에서 아르기닌 이외의 임의의 잔기의 아미노산 치환을 의미한다. 본원에서 대부분의 경우, 한 위치에서 특정 아미노산 잔기 차이는 "XnY"로서 표시되고, 여기서, "Xn"은 전술된 바와 같이 상응하는 위치를 특정하고, "Y"는 조작된 폴리펩타이드에서 발견된 아미노산(즉, 기준 폴리펩타이드의 잔기와 상이한 잔기)의 단일 문자 식별기호이다. (예를 들어, 표 2-1, 5-1, 6-1, 7-1, 8-1, 9-1, 11-1, 12-1, 및 13-1에 나타낸 바와 같이) 일부 경우, 본 개시는 통상의 표기법 "AnB"로 표시된 특정 아미노산 차이도 제공하고, 여기서, A는 기준 서열의 잔기의 단일 문자 식별기호이고, "n"은 기준 서열의 잔기 위치의 번호이고, B는 조작된 폴리펩타이드의 서열에서 잔기 치환의 단일 문자 식별기호이다. 일부 경우, 본 개시의 폴리펩타이드는 기준 서열에 비해 하나 이상의 아미노산 잔기 차이를 포함할 수 있고, 이것은 기준 서열에 비해 잔기 차이가 존재하는 특정된 위치의 목록에 의해 표시된다. 일부 실시양태에서, 하나 초과의 아미노산이 폴리펩타이드의 특정 잔기 위치에서 사용될 수 있는 경우, 사용될 수 있는 다양한 아미노산 잔기들은 "/"에 의해 분리된다(예를 들어, X247D/X247N 또는 X247D/N). 일부 실시양태에서, 효소 변이체는 하나 초과의 치환들을 포함한다. 이 치환들은 용이하게 읽히도록 슬래시에 의해 분리된다(예를 들어, D24S/D202N). 본 출원은 보존적 아미노산 치환 및 비보존적 아미노산 치환 중 어느 하나 또는 둘 다를 포함하는 하나 이상의 아미노산 차이를 포함하는 조작된 폴리펩타이드 서열을 포함한다.
본원에서 사용된 바와 같이, "돌연변이"는 폴리펩타이드 및 폴리뉴클레오타이드 서열의 치환, 삽입, 결실, 및 다른 변형을 지칭한다. 본 발명은 임의의 특정 유형의 돌연변이(들)로 제한되는 것으로 의도되지 않는다.
"보존적 아미노산 치환"은 잔기가 유사한 측쇄를 가진 상이한 잔기로 치환되는 것을 지칭하므로, 전형적으로 폴리펩타이드의 아미노산이 동일한 또는 유사한 규정된 아미노산 부류 내의 아미노산으로 치환되는 것을 포함한다. 제한이 아닌 예로써, 지방족 측쇄를 가진 아미노산은 또 다른 지방족 아미노산(예를 들어, 알라닌, 발린, 류신 및 이소류신)으로 치환될 수 있고/있거나; 하이드록실 측쇄를 가진 아미노산은 하이드록실 측쇄를 가진 또 다른 아미노산(예를 들어, 세린 및 쓰레오닌)으로 치환되고/되거나; 방향족 측쇄를 가진 아미노산은 방향족 측쇄를 가진 또 다른 아미노산(예를 들어, 페닐알라닌, 티로신, 트립토판 및 히스티딘)으로 치환되고/되거나; 염기성 측쇄를 가진 아미노산은 염기성 측쇄를 가진 또 다른 아미노산(예를 들어, 라이신 및 아르기닌)으로 치환되고/되거나; 산성 측쇄를 가진 아미노산은 산성 측쇄를 가진 또 다른 아미노산(예를 들어, 아스파르트산 또는 글루탐산)으로 치환되고/되거나; 소수성 또는 친수성 아미노산은 각각 또 다른 소수성 또는 친수성 아미노산으로 대체된다.
"비보존적 치환"은 폴리펩타이드의 아미노산이 유의미하게 상이한 측쇄 성질을 가진 아미노산으로 치환되는 것을 지칭한다. 비보존적 치환은 규정된 군 내에서 아미노산을 사용하기보다는 오히려 규정된 군들 사이에 아미노산을 사용할 수 있고, (a) 치환 영역에서 펩타이드 골격의 구조(예를 들어, 글리신 대신에 프롤린), (b) 전하 또는 소수성, 또는 (c) 측쇄의 부피에 영향을 미친다. 제한이 아닌 예로써, 예시적인 비보존적 치환은 염기성 또는 지방족 아미노산으로 치환된 산성 아미노산; 작은 아미노산으로 치환된 방향족 아미노산; 및 소수성 아미노산으로 치환된 친수성 아미노산일 수 있다.
본원에서 사용된 바와 같이, "결실"은 기준 폴리펩타이드로부터 하나 이상의 아미노산을 제거함으로써 폴리펩타이드를 변형시키는 것을 의미한다. 결실은 효소 활성을 보유하고/하거나 조작된 효소의 개선된 성질을 보유하면서, 1개 이상의 아미노산, 2개 이상의 아미노산, 5개 이상의 아미노산, 10개 이상의 아미노산, 15개 이상의 아미노산 또는 20개 이상의 아미노산, 아미노산의 총수의 최대 10%, 또는 기준 효소를 구성하는 아미노산의 총수의 최대 20%를 제거하는 것을 포함할 수 있다. 결실은 폴리펩타이드의 내부 부분 및/또는 말단 부분에 대해 유도될 수 있다. 다양한 실시양태에서, 결실은 연속 분절을 포함할 수 있거나 불연속적일 수 있다.
본원에서 사용된 바와 같이, "삽입"은 기준 폴리펩타이드로부터 하나 이상의 아미노산을 추가함으로써 폴리펩타이드를 변형시키는 것을 지칭한다. 삽입은 폴리펩타이드의 내부 부분, 또는 카르복시 또는 아미노 말단에서 일어날 수 있다. 본원에서 사용된 삽입은 당분야에서 공지되어 있는 바와 같이 융합 단백질을 포함한다. 삽입은 천연 생성 폴리펩타이드에서 아미노산의 연속 분절일 수 있거나 하나 이상의 아미노산에 의해 분리될 수 있다.
본원에서 교환 가능하게 사용된 "기능적 단편" 또는 "생물학적 활성 단편"은 아미노 말단 및/또는 카르복시 말단 결실(들) 및/또는 내부 결실을 갖지만, 전장 폴리펩타이드의 실질적으로 모든 활성을 보유하는 폴리펩타이드를 지칭하고, 여기서, 남은 아미노산 서열은 그와 비교되는 서열(예를 들어, 본 발명의 전장의 조작된 GLA)에서 상응하는 위치와 동일하다.
본원에서 사용된 바와 같이, "단리된 폴리펩타이드"는 천연적으로 동반된 다른 오염물질(예를 들어, 단백질, 지질 및 폴리뉴클레오타이드)로부터 실질적으로 분리된 폴리펩타이드를 지칭한다. 상기 용어는 천연 생성 환경 또는 발현 시스템(예를 들어, 숙주 세포 또는 시험관내 합성)으로부터 회수되거나 정제된 폴리펩타이드를 포괄한다. 재조합 GLA 폴리펩타이드는 세포 내부에 존재할 수 있거나, 세포 배지에 존재할 수 있거나, 다양한 형태, 예컨대, 용해물 또는 단리된 제제로 제조될 수 있다. 따라서, 일부 실시양태에서, 재조합 GLA 폴리펩타이드는 단리된 폴리펩타이드일 수 있다.
본원에서 사용된 바와 같이, "실질적으로 순수한 폴리펩타이드"는 폴리펩타이드 종이 존재하는 우성 종인 조성물을 지칭하고(즉, 이 폴리펩타이드 종은 몰 또는 중량을 기준으로 조성물에서 임의의 다른 개별 거대분자 종보다 더 풍부함), 일반적으로 목표 종이 몰 또는 % 중량을 기준으로 존재하는 거대분자 종의 적어도 약 50%를 차지할 때 실질적으로 정제된 조성물이다. 일반적으로, 실질적으로 순수한 GLA 조성물은 조성물에 존재하는 몰 또는 % 중량을 기준으로 모든 거대분자 종들의 약 60% 이상, 약 70% 이상, 약 80% 이상, 약 90% 이상, 약 95% 이상 및 약 98% 이상을 차지한다. 일부 실시양태에서, 목표 종은 본질적으로 균질한 정도까지 정제되고(즉, 오염물질 종은 통상의 검출 방법에 의해 조성물에서 검출될 수 없음), 여기서, 조성물은 본질적으로 단일 거대분자 종으로 구성된다. 용매 종, 소분자(<500 달톤) 및 원소 이온 종은 거대분자 종으로서 간주되지 않는다. 일부 실시양태에서, 단리된 재조합 GLA 폴리펩타이드는 실질적으로 순수한 폴리펩타이드 조성물이다.
본원에서 사용된 바와 같이, "개선된 효소 성질"은 기준 GLA 폴리펩타이드 및/또는 야생형 GLA 폴리펩타이드 또는 또 다른 조작된 GLA 폴리펩타이드에 비해 임의의 효소 성질의 개선을 나타내는 조작된 GLA 폴리펩타이드를 지칭한다. 개선된 성질은 증가된 유전자 발현, 증가된 단백질 생성, 증가된 열활성, 증가된 열안정성, 다양한 pH 수준에서의 증가된 활성, 증가된 안정성, 증가된 효소 활성, 증가된 기질 특이성 또는 친화성, 증가된 특이적 활성, 기질 및/또는 생성물 억제에 대한 증가된 내성, 증가된 화학적 안정성, 개선된 화학선택성, 개선된 용매 안정성, 산성, 중성 또는 염기성 pH에 대한 증가된 내성, 단백질용해 활성에 대한 증가된 내성(즉, 단백질용해에 대한 감소된 민감성), 감소된 응집, 증가된 가용성, 감소된 면역원성, 개선된 번역 후 변형(예를 들어, 글리코실화), 변경된 온도 프로파일, 증가된 세포 흡수, 증가된 라이소좀 안정성, Gb3의 세포를 고갈시키는 증가된 능력, GLA 생성 세포로부터의 증가된 분비 등과 같은 성질을 포함하나, 이들로 제한되지 않는다.
본원에서 사용된 바와 같이, "증가된 효소 활성" 또는 "향상된 촉매 활성"은 기준 GLA 효소에 비해 특이적 활성의 증가(예를 들어, 생성된 생성물/시간/중량 단백질) 또는 생성물로의 기질의 퍼센트 전환의 증가(예를 들어, 특정된 양의 GLA를 사용할 때 특정된 시간 이내에 출발 양의 기질이 생성물로 전환되는 퍼센트)에 의해 표시될 수 있는, 조작된 GLA 폴리펩타이드의 개선된 성질을 의미한다.
효소 활성을 측정하는 예시적인 방법은 실시예에 제공되어 있다. 고전적인 효소 성질인 Km, Vmax 또는 kcat를 비롯한, 효소 활성과 관련된 임의의 성질이 영향을 받을 수 있고, 이의 변화는 증가된 효소 활성으로 이어질 수 있다. 효소 활성의 개선은 상응하는 야생형 효소의 효소 활성의 약 1.1배부터, GLA 폴리펩타이드가 유도된 천연 생성 GLA 또는 또 다른 조작된 GLA보다 2배, 5배, 10배, 20배, 25배, 50배, 75배, 100배, 150배, 200배 또는 더 큰 효소 활성에 이르는 개선일 수 있다.
일부 실시양태에서, 조작된 GLA 폴리펩타이드는 적어도 0.1/초, 적어도 0.5/초, 적어도 1.0/초, 적어도 5.0/초, 적어도 10.0/초의 kcat, 및 일부 바람직한 실시양태에서 10.0/초 초과의 kcat를 가진다. 일부 실시양태에서, Km은 약 1 μM 내지 약 5 mM의 범위; 약 5 μM 내지 약 2 mM의 범위; 약 10 μM 내지 약 2 mM의 범위; 또는 약 10 μM 내지 약 1 mM의 범위 내에 있다. 일부 특정 실시양태에서, 조작된 GLA 효소는 특정 조건에 노출된 후 기준 GLA 효소(예를 들어, 야생형 GLA 또는 임의의 다른 기준 GLA, 예컨대, 서열번호 8)의 효소 활성보다 1.5배 내지 10배, 1.5배 내지 25배, 1.5배 내지 50배, 1.5배 내지 100배 또는 더 큰 범위의 개선된 효소 활성을 나타낸다. GLA 활성은 당분야에서 공지되어 있는 임의의 적합한 방법(예를 들어, 표준 어세이, 예컨대, 반응물 또는 생성물의 분광광도측정 성질의 변화 모니터링)에 의해 측정될 수 있다. 일부 실시양태에서, 생성된 생성물의 양은 직접적으로 또는 o-프탈디알데하이드(OPA) 유도체화 후 UV 흡광도 또는 형광 검출과 병용된 고성능 액체 크로마토그래피(HPLC) 분리에 의해 측정될 수 있다. 일부 실시양태에서, 생성된 생성물의 양은 4-메틸움벨리페릴-알파-D-갈락토피라노사이드(4-MUGal) 분자의 가수분해 후 형광(여기 355 nm, 방출 460 nm)을 모니터링함으로써 측정될 수 있다. 본원에 더 상세히 기재된 바와 같이, 규정된 효소 제제, 설정된 조건 하에서의 규정된 어세이, 및 하나 이상의 규정된 기질을 사용함으로써 효소 활성을 비교한다. 일반적으로, 용해물을 비교할 때, 어세이되는 세포의 수 및 단백질의 양뿐만 아니라, 숙주 세포에 의해 생성되고 용해물에 존재하는 효소 양의 변화를 최소화하기 위한 동일한 발현 시스템 및 동일한 숙주 세포의 사용도 확인한다.
본원에서 사용된 바와 같이, 용어 "산성 pH에 대한 개선된 내성"은 본 발명에 따른 재조합 GLA가 기준 GLA 또는 또 다른 효소에 비해 증가된 안정성(특정된 시간(1시간, 최대 24시간) 동안 산성 pH에 노출된 후 약 pH 4.8에서 더 높은 보유된 활성)을 가질 것임을 의미한다.
본원에서 사용된 바와 같이, 용어 "개선된 세포 흡수"는 본원에 제공된 재조합 GLA가 기준 GLA(야생형 GLA를 포함함) 또는 또 다른 효소에 비해 세포 내로의 증가된 세포내이입을 나타냄을 의미한다. 일부 실시양태에서, 세포는 배양된 파브리 환자 섬유모세포(기준 GLA 또는 또 다른 효소에 비해 특정 시간에 걸쳐 배양된 세포와 함께 인큐베이션된 후 더 높은 보유된 세포내 활성)이다. 일부 추가 실시양태에서, 본원에 제공된 재조합 GLA는 기준 GLA(야생형 GLA를 포함함) 또는 또 다른 효소에 비해 특정 시간에 걸쳐 배양된 세포와 함께 더 큰 보유된 세포내 활성을 나타낸다. 일부 추가 실시양태에서, 시간은 약 4시간인 반면, 일부 다른 실시양태에서 시간은 4시간 미만의 시간(예를 들어, 1시간, 2시간 또는 3시간)이고, 일부 대안적 실시양태에서 시간은 4시간 초과(예를 들어, 5시간, 6시간, 7시간, 8시간 이상)이다.
본원에서 사용된 바와 같이, "생리학적 pH"는 대상체(예를 들어, 인간) 혈액에서 일반적으로 발견되는 pH 범위를 의미한다.
(예를 들어, 염기성 pH 조건에 대한 개선된 안정성 또는 염기성 pH에 대한 증가된 내성과 관련하여 사용된) 용어 "염기성 pH"는 약 7 내지 11의 pH 범위를 의미한다.
(예를 들어, 산성 pH 조건에 대한 개선된 안정성 또는 산성 pH에 대한 증가된 내성과 관련하여 사용된) 용어 "산성 pH"는 약 1.5 내지 4.5의 pH 범위를 의미한다.
본원에서 사용된 바와 같이, "전환"은 기질(들)이 효소에 의해 상응하는 생성물(들)로 전환되는 것(또는 생물변환되는 것)을 지칭한다. "퍼센트 전환"은 특정된 조건 하에서 일정 시간 이내에 생성물로 전환되는 기질의 퍼센트를 지칭한다. 따라서, GLA 폴리펩타이드의 "효소 활성" 또는 "활성"은 특정 시간 이내에 생성물로의 기질의 "퍼센트 전환"으로서 표현될 수 있다.
본원에서 사용된 바와 같이, "하이브리드화 엄격도"는 핵산의 하이브리드화에 있어서 세척 조건과 같은 하이브리드화 조건을 의미한다. 일반적으로, 하이브리드화 반응을 보다 낮은 엄격도의 조건 하에서 수행한 후, 상이하되 보다 더 높은 엄격도의 세척을 수행한다. 용어 "적당한 엄격도 하이브리드화"는 표적 DNA가 표적 폴리뉴클레오타이드에 대한 약 90% 초과의 동일성과 함께 표적 DNA에 대한 약 60% 동일성, 바람직하게는 약 75% 동일성, 약 85% 동일성을 가진 상보적 핵산에 결합하게 하는 조건을 지칭한다. 예시적인 적당한 엄격도 조건은 42℃에서 50% 포름아미드, 5X 덴하르트 용액, 5X SSPE, 0.2% SDS에서 하이브리드화시킨 후 42℃에서 0.2X SSPE, 0.2% SDS에서 세척하는 조건과 동등한 조건이다. "고엄격도 하이브리드화"는 일반적으로 규정된 폴리뉴클레오타이드 서열에 대한 용액 조건 하에서 측정될 때 열 용융 온도 Tm보다 약 10℃ 이하만큼 더 낮은 조건을 지칭한다. 일부 실시양태에서, 고엄격도 조건은 65℃에서 0.018 M NaCl에서 안정한 하이브리드를 형성하는 핵산 서열만의 하이브리드화를 허용하는 조건을 지칭한다(즉, 본원에서 예상된 바와 같이, 하이브리드가 65℃에서 0.018 M NaCl에서 안정하지 않은 경우, 이것은 고엄격도 조건 하에서 안정하지 않을 것이다). 예를 들어, 고엄격도 조건은 42℃에서 50% 포름아미드, 5X 덴하르트 용액, 5X SSPE 및 0.2% SDS와 동등한 조건에서 하이브리드화시킨 후, 65℃에서 0.1X SSPE 및 0.1% SDS에서 세척함으로써 제공될 수 있다. 또 다른 고엄격도 조건은 65℃에서 0.1%(w:v) SDS를 함유하는 5X SSC에서 하이브리드화시키는 것과 동등한 조건에서 하이브리드화시키고 65℃에서 0.1% SDS를 함유하는 0.1X SSC에서 세척하는 것이다. 다른 고엄격도 하이브리드화 조건뿐만 아니라 적당한 엄격도 조건은 상기 인용된 참고문헌에 기재되어 있다.
본원에서 사용된 바와 같이, "코돈 최적화된"은 코딩된 단백질이 관심 있는 유기체 및/또는 세포에서 더 효율적으로 발현되도록 단백질을 코딩하는 폴리뉴클레오타이드의 코돈의 변화를 의미한다. 유전 코드는 대다수의 아미노산들이 "동의어(synonyms)" 또는 "동의어(synonymous)" 코돈으로서 불리는 여러 코돈들에 의해 표시된다는 점에서 축퇴되어 있지만, 특정 유기체에 의한 코돈 사용은 무작위적이지 않고 특정 코돈 삼중체에 대해 편향되어 있다는 것은 잘 공지되어 있다. 이러한 코돈 사용 편향은 소정의 유전자, 공통된 기능 또는 조상 기원의 유전자, 고도로 발현된 단백질 대 낮은 카피 수 단백질, 및 유기체 게놈의 응집체 단백질 코딩 영역과 관련하여 더 높을 수 있다. 일부 실시양태에서, GLA 효소를 코딩하는 폴리뉴클레오타이드는 GC 함량을 차지하는 발현, 숨겨진 스플라이스 부위, 전사 종결 신호, RNA 안정성에 영향을 미칠 수 있는 모티프 및 핵산 이차 구조뿐만 아니라 관심 있는 임의의 다른 요인에 따라 선택된 숙주 유기체(들) 및/또는 세포 유형(들)로부터의 최적 생성을 위해 코돈-최적화될 수 있다.
본원에서 사용된 바와 같이, "조절 서열"은 본 출원의 폴리뉴클레오타이드 및/또는 폴리펩타이드의 발현에 필요하거나 유리한 모든 성분들을 포함하도록 지칭된다. 각각의 조절 서열은 폴리펩타이드를 코딩하는 핵산 서열에 대한 천연 또는 외래 서열일 수 있다. 이러한 조절 서열은 리더, 폴리아데닐화 서열, 전구펩타이드 서열, 프로모터 서열, 신호 펩타이드 서열, 시작 서열 및 전사 종결자를 포함하나, 이들로 제한되지 않는다. 최소한, 조절 서열은 프로모터, 및 전사 및 번역 정지 신호를 포함한다. 조절 서열은 조절 서열과, 폴리펩타이드를 코딩하는 핵산 서열의 코딩 영역의 라이게이션을 용이하게 하는 특정 제한 부위를 도입할 목적으로 링커를 구비할 수 있다.
본원에서 사용된 바와 같이, "작동 가능하게 연결된"은 본원에서 조절 서열이 관심 있는 폴리뉴클레오타이드 및/또는 폴리펩타이드의 발현을 유도하거나 조절하도록 조절 서열이 관심 있는 폴리뉴클레오타이드와 관련된 위치에서 적절하게(즉, 기능적 관계로) 배치되는 배열을 지칭한다.
본원에서 사용된 바와 같이, "프로모터 서열"은 관심 있는 폴리뉴클레오타이드, 예컨대, 코딩 서열의 발현을 위해 숙주 세포에 의해 인식되는 핵산 서열을 지칭한다. 프로모터 서열은 관심 있는 폴리뉴클레오타이드의 발현을 매개하는 전사 조절 서열을 함유한다. 프로모터는 돌연변이체 프로모터, 절단된 프로모터 및 하이브리드 프로모터를 비롯한, 선택된 숙주 세포에서 전사 활성을 보이는 임의의 핵산 서열일 수 있고, 숙주 세포에 대한 동종성 또는 이종성을 가진 세포외 또는 세포내 폴리펩타이드를 코딩하는 유전자로부터 수득될 수 있다.
본원에서 사용된 바와 같이, "적합한 반응 조건"은 본 출원의 GLA 폴리펩타이드가 기질을 원하는 생성물 화합물로 전환시킬 수 있는, 효소 전환 반응 용액에서의 조건(예를 들어, 효소 로딩의 범위, 온도, pH, 완충제, 보조용매 등)을 지칭한다. 예시적인 "적합한 반응 조건"은 본 출원에 제공되고 실시예에 의해 예시된다. "효소 로딩"은 반응 시작 시 반응 혼합물 중의 성분의 농도 또는 양을 지칭한다. 효소 전환 반응 과정과 관련하여 "기질"은 GLA 폴리펩타이드가 작용하는 화합물 또는 분자를 지칭한다. 효소 전환 과정과 관련하여 "생성물"은 GLA 폴리펩타이드가 기질에 작용함으로써 생성된 화합물 또는 분자를 지칭한다.
본원에서 사용된 바와 같이, 용어 "배양"은 (예를 들어, 액체, 겔 또는 고체 배지를 사용하여) 임의의 적합한 조건 하에서 미생물, 포유류 또는 다른 적합한 세포의 집단을 생장시키는 것을 지칭한다.
재조합 폴리펩타이드는 당분야에서 공지되어 있는 임의의 적합한 방법을 이용함으로써 생성될 수 있다. 관심 있는 야생형 폴리펩타이드를 코딩하는 유전자는 벡터, 예컨대, 플라스미드에 클로닝될 수 있고, 원하는 숙주, 예컨대, 이. 콜라이(E. coli), 에스. 세레비지애(S. cerevisiae) 또는 포유류 세포주(예를 들어, HEK 또는 CHO 세포) 등에서 발현될 수 있다. 재조합 폴리펩타이드의 변이체는 당분야에서 공지되어 있는 다양한 방법들에 의해 생성될 수 있다. 실제로, 당분야에서 숙련된 자에게 잘 공지되어 있는 매우 다양한 상이한 돌연변이유발 기법들이 있다. 추가로, 돌연변이유발 키트도 많은 시판되는 분자생물학 공급자들로부터 입수될 수 있다. 규정된 아미노산 위치에서 특정 치환을 만들거나(부위 지정), 유전자의 국소화된 영역에서 특정 또는 무작위 돌연변이를 만들거나(영역 특이적) 또는 전체 유전자에 걸쳐 무작위 돌연변이유발(예를 들어, 포화 돌연변이유발)을 야기하는 방법이 이용될 수 있다. PCR을 이용한 단일 가닥 DNA 또는 이중 가닥 DNA의 부위 지정 돌연변이유발, 카세트 돌연변이유발, 유전자 합성, 오류 유발 PCR, 셔플링 및 화학적 포화 돌연변이유발, 또는 당분야에서 공지되어 있는 임의의 다른 적합한 방법을 포함하나 이들로 제한되지 않는, 효소 변이체를 생성하는 다수의 적합한 방법들이 당업자에게 공지되어 있다. DNA 및 단백질 조작을 위해 이용되는 방법의 비제한적인 예는 하기특허들에서 제공되어 있다: 미국 특허 제6,117,679호; 미국 특허 제6,420,175호; 미국 특허 제6,376,246호; 미국 특허 제6,586,182호; 미국 특허 제7,747,391호; 미국 특허 제7,747,393호; 미국 특허 제7,783,428호; 및 미국 특허 제8,383,346호. 변이체가 생성된 후, 임의의 원하는 성질(예를 들어, 높거나 증가된 활성, 낮거나 감소된 활성, 증가된 열활성, 증가된 열안정성, 및/또는 산성 pH 안정성 등)에 대해 변이체를 스크리닝할 수 있다. 일부 실시양태에서, "재조합 GLA 폴리펩타이드"(본원에서 "조작된 GLA 폴리펩타이드", "변이체 GLA 효소" 및 "GLA 변이체"로서도 지칭됨)가 사용된다.
본원에서 사용된 바와 같이, "벡터"는 DNA 서열을 세포 내로 도입하기 위한 DNA 작제물이다. 일부 실시양태에서, 벡터는 적합한 숙주에서 DNA 서열에 코딩된 폴리펩타이드의 발현을 달성할 수 있는 적합한 조절 서열에 작동 가능하게 연결된 발현 벡터이다. 일부 실시양태에서, "발현 벡터"는 숙주 세포에서 발현을 유도하기 위해 DNA 서열(예를 들어, 형질전이유전자)에 작동 가능하게 연결된 프로모터 서열을 갖고, 일부 실시양태에서 전사 종결자 서열도 포함한다.
본원에서 사용된 바와 같이, 용어 "유전자 요법 벡터"는 폴리뉴클레오타이드 서열을 세포에게 전달하기에 적합한 비히클 또는 담체를 지칭한다. 일부 실시양태에서, 아데노바이러스(AV), 아데노-관련 바이러스(AAV), 렌티바이러스(LV) 및 비-바이러스 벡터, 예컨대, 리포좀을 포함하나 이들로 제한되지 않는 벡터는 세포 또는 조직에게 전달하기 위해 유전자(예를 들어, 치료 유전자) 또는 폴리뉴클레오타이드 서열을 캡슐화한다. 소정의 상황에 적합한 임의의 비히클이 사용되기 때문에, 본 발명은 임의의 특정 유전자 요법 벡터로 제한되는 것으로 의도되지 않는다. 유전자 요법 벡터는 유전자를 특정 종 또는 숙주에게 전달하도록 설계될 수 있거나, 더 일반적으로 적용될 수 있다.
본원에서 사용된 바와 같이, 용어 "발현"은 전사, 전사 후 변형, 번역 및 번역 후 변형을 포함하나 이들로 제한되지 않는, 폴리펩타이드의 생성과 관련된 임의의 단계를 포함한다. 일부 실시양태에서, 용어는 세포로부터의 폴리펩타이드의 분비도 포괄한다.
본원에서 사용된 바와 같이, 용어 "생성한다"는 세포에 의한 단백질 및/또는 다른 화합물의 생성을 지칭한다. 상기 용어는 전사, 전사 후 변형, 번역 및 번역 후 변형을 포함하나 이들로 제한되지 않는, 폴리펩타이드의 생성과 관련된 임의의 단계를 포괄하는 것으로 의도된다. 일부 실시양태에서, 상기 용어는 세포로부터의 폴리펩타이드의 분비도 포괄한다.
본원에서 사용된 바와 같이, 2개의 서열들이 천연적으로 연결되지 않는 경우, 아미노산 또는 뉴클레오타이드 서열(예를 들어, 프로모터 서열, 신호 펩타이드, 종결자 서열 등)은 그 자신에 작동 가능하게 연결된 또 다른 서열에 대한 "재조합" 또는 "이종" 서열이다.
본원에서 사용된 바와 같이, 용어 "숙주 세포" 및 "숙주 균주"는 본원에 제공된 DNA(예를 들어, GLA 변이체를 코딩하는 폴리뉴클레오타이드)를 포함하는 발현 벡터에 적합한 숙주를 지칭한다. 일부 실시양태에서, 숙주 세포는 당분야에서 공지된 바와 같은 재조합 DNA 기법을 이용함으로써 작제된 벡터에 의해 형질전환되었거나 형질감염된 원핵세포 또는 진핵세포이다.
용어 "유사체"는 기준 폴리펩타이드에 대한 70% 초과의 서열 동일성 내지 100% 미만의 서열 동일성(예를 들어, 75%, 78%, 80%, 83%, 85%, 88%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 초과의 서열 동일성)을 가진 폴리펩타이드를 의미한다. 일부 실시양태에서, "유사체"는 천연 생성 아미노산뿐만 아니라 호모아르기닌, 오르니틴 및 노르발린을 포함하나 이들로 제한되지 않는 하나 이상의 비천연 생성 아미노산 잔기도 함유하는 폴리펩타이드를 의미한다. 일부 실시양태에서, 유사체는 2개 이상의 아미노산 잔기들 사이의 하나 이상의 D-아미노산 잔기 및 비-펩타이드 결합도 포함한다.
본원에서 사용된 바와 같이, 용어 "치료제"는 유리한 또는 바람직한 의학적 효과를 가진, 병리학적 징후 또는 증상을 보이는 대상체에게 투여되는 화합물을 지칭한다.
본원에서 사용된 바와 같이, 용어 "약학 조성물"은 본 발명에 의해 포괄되는 약학적 유효량의 조작된 GLA 폴리펩타이드 및 허용 가능한 담체를 포함하는, 포유류 대상체(예를 들어, 인간)에서 약학적으로 사용되기에 적합한 조성물을 지칭한다.
용어 "유전자 요법"은 질환의 치료 또는 예방을 위해 유전자 요법 벡터로 유전자, 폴리데옥시리보뉴클레오타이드 또는 폴리뉴클레오타이드 서열(들)을 세포 또는 조직에게 전달하여 상기 세포 또는 조직을 변형시키는 것을 지칭한다. 유전자 요법은 질환을 야기하는 돌연변이된 유전자를 이 유전자의 건강한 카피로 대체하거나, 부적절하게 작용하는 돌연변이된 유전자를 불활성화시키거나 "넉아웃시키는" 것을 포함할 수 있다. 일부 실시양태에서, 유전자 요법은 환자에서 질환을 치료하는 데 이용된다.
본원에서 사용된 바와 같이, 용어 "mRNA 요법"은 질환의 치료 또는 예방을 위해 mRNA 폴리리보뉴클레오타이드 서열을 세포 또는 조직에게 전달하여 상기 세포 또는 조직을 변형시키는 것을 지칭한다. 일부 실시양태에서, 세포 또는 조직에게 전달될 mRNA 폴리뉴클레오타이드 서열은 예를 들어, 리포좀으로 제형화되나, 이것으로 제한되지 않는다. 일부 실시양태에서, mRNA 요법은 환자에서 질환을 치료하는 데 이용된다.
본원에서 사용된 바와 같이, 용어 "세포 요법"은 질환의 치료 또는 예방을 위해 외생적으로 변형된 살아있는 세포를 환자에게 전달하여 누락된 유전자를 제공하는 것을 지칭한다. 그 다음, 변형된 세포는 체내로 재도입된다.
본원에서 사용된 바와 같이, 용어 "유효량"은 원하는 결과를 생성하기에 충분한 양을 의미한다. 당분야에서 일반적인 기술을 가진 자는 관용적인 실험을 이용함으로써 유효량을 확인할 수 있다.
용어 "단리된" 및 "정제된"은 그 자신과 천연적으로 연결되어 있는 적어도 하나의 다른 성분으로부터 제거되어 있는 분자(예를 들어, 단리된 핵산, 폴리펩타이드 등) 또는 다른 성분을 지칭하기 위해 본원에서 사용된다. 용어 "정제된"은 절대 순도를 요구하지 않고, 오히려 상대적인 정의로 의도된다.
본원에서 사용된 바와 같이, 용어 "대상체"는 인간, 비인간 영장류, 가축, 애완 동물 및 실험실 동물(예를 들어, 설치류 및 토끼목 포유류)과 같은 포유류를 포괄한다. 상기 용어는 수컷뿐만 아니라 암컷도 포괄하는 것으로 의도된다.
본원에서 사용된 바와 같이, 용어 "환자"는 질환에 대해 평가받고 있거나 치료받고 있거나, 질환을 앓고 있는 임의의 대상체를 의미한다.
용어 "유아"는 출생 후 첫 달부터 대략 한(1)살까지의 기간에 있는 어린이를 지칭한다. 본원에서 사용된 바와 같이, 용어 "신생아"는 출생부터 생후 28일째 날까지의 기간에 있는 어린이를 지칭한다. 용어 "미숙아"는 임신 20주차를 채웠으나 만삭 전에 태어난 유아로서, 일반적으로 출생 시 체중이 약 500 내지 약 2499 그램인 유아를 지칭한다. "초저체중 출생아"는 출생 시 체중이 1500 g 미만인 유아이다.
본원에서 사용된 바와 같이, 용어 "소아"는 치료 또는 연구 절차에 동의하기 위한 법적 연령에 도달하지 않은 사람을 지칭한다. 일부 실시양태에서, 상기 용어는 출생 시기와 청소년기 사이의 사람을 지칭한다.
본원에서 사용된 바와 같이, 용어 "성인"은 관련 관할권에 대한 법적 연령(예를 들어, 미국에서 18세)에 도달한 사람을 지칭한다. 일부 실시양태에서, 상기 용어는 임의의 완전히 성장한 성숙 유기체를 지칭한다. 일부 실시양태에서, 용어 "청년"은 18세 미만이나 성적 성숙기에 도달한 사람을 지칭한다.
본원에서 사용된 바와 같이, "조성물" 및 "제형"은 임의의 적합한 용도로 의도된, 적어도 하나의 본 발명의 조작된 GLA를 포함하는 생성물(예를 들어, 약학 조성물, 식이/영양 보충제, 식품 등)을 포괄한다.
본원에서 사용된 바와 같이, 용어 조성물의 "투여" 및 조성물을 "투여하는"은 본 발명의 조성물을 대상체(예를 들어, 파브리병의 효과로부터 고통받는 사람)에게 제공하는 것을 의미한다.
본원에서 사용된 바와 같이, 약학 조성물과 관련하여 사용될 때 용어 "담체"는 임의의 표준 약학 담체, 완충제 및 부형제, 예컨대, 안정화제, 보존제 및 보조제를 의미한다.
본원에서 사용된 바와 같이, 용어 "약학적으로 허용 가능한"은 임의의 바람직하지 않은 생물학적 효과를 야기하지 않거나 함유된 성분들 중 임의의 성분과 유해한 방식으로 상호작용하지 않으면서 대상체에게 투여될 수 있고 원하는 생물학적 활성을 가진 물질을 의미한다.
본원에서 사용된 바와 같이, 용어 "부형제"는 활성 약학 성분(API; 예를 들어, 본 발명의 조작된 GLA 폴리펩타이드)을 제외한 임의의 약학적으로 허용 가능한 첨가제, 담체, 희석제, 보조제 또는 다른 성분을 지칭한다. 부형제는 전형적으로 제형화 및/또는 투여 목적을 위해 포함된다.
본원에서 사용된 바와 같이, 질환/병태의 증상과 관련하여 사용될 때 용어 "치료적 유효량"은 질환/병태의 하나 이상의 증상을 호전시키거나, 약화시키거나, 제거하거나, 증상(들)의 발병을 예방하거나 지연시키는, 화합물(예를 들어, 조작된 GLA 폴리펩타이드)의 양 및/또는 농도를 지칭한다.
질환/병태와 관련하여 사용될 때 용어 "치료적 유효량"은 질환/병태를 호전시키거나, 약화시키거나, 제거하는, 조성물(예를 들어, 조작된 GLA 폴리펩타이드)의 양 및/또는 농도를 지칭한다. 일부 실시양태에서, 상기 용어는 연구원, 의사, 수의사 또는 다른 임상의가 찾는, 조직, 시스템 또는 동물 대상체에 의한 생물학적(예를 들어, 의학적) 반응을 이끌어내는 조성물의 양을 지칭하는 데 사용된다.
용어 "치료하는", "치료한다" 및 "치료"는 경감적 치료뿐만 아니라 방지적(예를 들어, 예방적) 치료도 포괄하기 위한 것이다.
조작된 GLA 발현 및 활성:
합성 마우스 IG 신호 펩타이드를 사용하여 효모 코돈 최적화된 성숙 인간 GLA의 분비된 발현을 달성하였다. HEK293T 세포에서 pCDNA3.1(+) 벡터로부터 클론을 발현하였다. 이 접근법은 형광생성 기질 4-메틸움벨리페릴 α-D-갈락토피라노사이드(4-MuGal)에 대한 측정 가능한 활성을 가진 상청액을 제공하였다.
일부 실시양태에서, 서열번호 8에 비해 유사한 안정성 및 개선된 세포 흡수를 가진 돌연변이 다양성을 확인하기 위해, 서열번호 8로부터 유도된 GLA 변이체를 생성하는 조합 라이브러리를 작제하였다. 동등한 부피의 상청액을 비챌린지 조건(인큐베이션 없음, pH 4.6)에서 스크리닝하였거나, 낮은 pH(3.9 내지 4.2), 중성 pH(7.0 내지 7.6) 또는 인간 혈청(생리학적 pH 7.1 내지 8.2) 환경에서 1시간 인큐베이션한 후 스크리닝하였다. 증가된 GLA 발현 또는 GLA 특이적 활성으로 인한 활성을 가진 GLA 변이체를 모 GLA에 비해 그의 배수 개선에 근거하여 확인하였다. 챌린지 조건 하에서 관측된 배수 개선을 비챌린지 조건 하에서 관측된 배수 개선으로 나눔으로써, 증가된 안정성을 가진 GLA 변이체를 확인하였다. 이 접근법은 pH 극한에서 특이적 활성의 변화 없이 증가된 발현에 근거한 변이체 선택에 대한 편향을 감소시킨다. 종합 활성 점수(모든 3개의 조건들에 대한 배수 개선의 곱) 및 안정성(안정성 점수의 곱)을 사용하여, 후속 GLA 라이브러리에 포함시킬 개선된 변이체에서 돌연변이의 순위를 매겼다. 추가 실시양태에서, 실시예에 기재된 추가 방법 및 서열을 사용하였다.
조작된 GLA:
일부 실시양태에서, 개선된 성질을 나타내는 조작된 GLA는 서열번호 2, 8, 48, 158, 372, 374, 704 및/또는 1022와 적어도 약 85%, 적어도 약 88%, 적어도 약 90%, 적어도 약 91%, 적어도 약 92%, 적어도 약 93%, 적어도 약 94%, 적어도 약 95%, 적어도 약 96%, 적어도 약 97%, 적어도 약 98%, 적어도 약 99% 또는 약 100%의 아미노산 서열 동일성, 및 하나 이상의 아미노산 위치(예컨대, 서열번호 2, 8, 48, 158, 372, 374, 704 및/또는 1022에 비해 1개, 2개, 3개, 4개, 5개, 6개, 7개, 8개, 9개, 10개, 11개, 12개, 14개, 15개, 20개 이상의 아미노산 위치, 또는 서열번호 2, 8, 48, 158, 372, 374, 704 및/또는 1022와 적어도 85%, 적어도 88%, 적어도 90%, 적어도 91%, 적어도 92%, 적어도 93%, 적어도 94%, 적어도 95%, 적어도 96%, 적어도 97%, 적어도 98%, 적어도 99% 이상의 아미노산 서열 동일성을 가지는 서열)에서 서열번호 2, 8, 48, 158, 372, 374, 704 및/또는 1022에 비해 아미노산 잔기 차이를 가진다. 일부 실시양태에서, 하나 이상의 위치에서 서열번호5에 비해 잔기 차이는 적어도 1개, 2개, 3개, 4개, 5개, 6개, 7개, 8개, 9개, 10개 이상의 보존적 아미노산 치환을 포함한다. 일부 실시양태에서, 조작된 GLA 폴리펩타이드는 표 2-1, 5-1, 6-1, 7-1, 8-1, 9-1, 11-1, 12-1, 및/또는 13-1에 나열된 폴리펩타이드이다. 일부 실시양태에서, 조작된 GLA 폴리펩타이드는 서열번호 2, 8, 48, 158, 372, 374, 704 및/또는 1022를 포함한다.
본 발명은 또한 서열번호 8에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 이상의 서열 동일성을 가지는 폴리펩타이드 서열 또는 이의 기능적 단편을 포함하는 재조합 알파-갈락토시다제 A를 제공하고, 여기서, 상기 재조합 알파-갈락토시다제 A는 44, 44/217, 44/217/316, 44/217/322, 44/217/322/337, 44/247, 44/247/302, 44/247/302/322, 44/247/322, 44/247/337, 44/247/362, 44/302, 44/337, 44/373, 217/322, 217/373, 247/322, 247/362, 302/322/362/373, 302/337, 316, 316/337, 322, 322/337, 362/373 및 373으로부터 선택된 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열번호 8을 기준으로 넘버링된다. 일부 실시양태에서, 재조합 알파-갈락토시다제 A는 서열번호 8에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 이상의 서열 동일성을 가지는 폴리펩타이드 서열 또는 이의 기능적 단편을 포함하고, 여기서, 상기 재조합 알파-갈락토시다제 A는 44L, 44L/217F, 44L/217F/316L, 44L/217F/322M, 44L/217F/322M/337A, 44L/247N, 44L/247N/302Q, 44L/247N/302Q/322M, 44L/247N/322M, 44L/247N/337A, 44L/247N/362K, 44L/302Q, 44L/337A, 44L/373R, 217F/322M, 217F/373R, 247N/322M, 247N/362K, 302Q/322M/362K/373R, 302Q/337A, 316L, 316L/337A, 322M, 322M/337A, 362K/373R 및 373R로부터 선택된 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열번호 8을 기준으로 넘버링된다. 일부 실시양태에서, 재조합 알파-갈락토시다제 A는 서열번호 8에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 이상의 서열 동일성을 가지는 폴리펩타이드 서열 또는 이의 기능적 단편을 포함하고, 여기서, 상기 재조합 알파-갈락토시다제 A는 R44L, R44L/R217F, R44L/R217F/D316L, R44L/R217F/I322M, R44L/R217F/I322M/P337A, R44L/D247N, R44L/D247N/K302Q, R44L/D247N/K302Q/I322M, R44L/D247N/I322M, R44L/D247N/P337A, R44L/D247N/Q362K, R44L/K302Q, R44L/P337A, R44L/K373R, R217F/I322M, R217F/K373R, D247N/I322M, D247N/Q362K, K302Q/I322M/Q362K/K373R, K302Q/P337A, D316L, D316L/P337A, I322M, I322M/P337A, Q362K/K373R 및 K373R로부터 선택된 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열번호 8을 기준으로 넘버링된다.
본 발명은 또한 서열번호 8에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 이상의 서열 동일성을 가지는 폴리펩타이드 서열 또는 이의 기능적 단편을 포함하는 재조합 알파-갈락토시다제 A를 제공하고, 여기서, 상기 재조합 알파-갈락토시다제 A는 10/39/44/47/92/166/206/217/247/261/271/302/316/322/337/362/368/373/392, 44/217/316, 44/217/322/337, 166/362, 217/373 및 362/373으로부터 선택된 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열번호 8을 기준으로 넘버링된다. 일부 실시양태에서, 재조합 알파-갈락토시다제 A는 서열번호 8에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 이상의 서열 동일성을 가지는 폴리펩타이드 서열 또는 이의 기능적 단편을 포함하고, 여기서, 상기 재조합 알파-갈락토시다제 A는 10T/39M/44L/47S/92Y/166S/206K/217F/247N/261A/271H/302Q/316L/322M/337A/362K/368W/373R/392M, 44L/217F/316L, 44L/217F/322M/337A, 166A/362K, 217F/373R 및 362K/373R로부터 선택된 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열번호 8을 기준으로 넘버링된다. 일부 실시양태에서, 재조합 알파-갈락토시다제 A는 서열번호 8에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 이상의 서열 동일성을 가지는 폴리펩타이드 서열 또는 이의 기능적 단편을 포함하고, 여기서, 상기 재조합 알파-갈락토시다제 A는 P10T/E39M/R44L/T47S/H92Y/P166S/A206K/R217F/D247N/G261A/A271H/K302Q/D316L/I322M/P337A/Q362K/A368W/K373R/T392M, R44L/R217F/D316L, R44L/R217F/I322M/P337A, P166A/Q362K, R217F/K373R 및 Q362K/K373R로부터 선택된 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열번호 8을 기준으로 넘버링된다.
본 발명은 또한 서열번호 58에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 이상의 서열 동일성을 가지는 폴리펩타이드 서열 또는 이의 기능적 단편을 포함하는 재조합 알파-갈락토시다제 A를 제공하고, 여기서, 상기 재조합 알파-갈락토시다제 A는 7, 7/48/68, 7/48/68/120/282/299, 7/48/130/282, 7/48/180, 7/68/130/282/365, 7/68/180, 7/88/120/305/365, 7/120, 7/130, 7/282, 7/305, 7/305/365, 7/365, 39, 47, 47/87/95/96/158/162, 47/95, 47/273, 47/343, 48, 48/68, 48/180/282, 48/282, 48/282/305, 67/180, 68, 68/299/300, 71, 87/91/95/96/158/162, 87/91/95/96/206/343, 87/96/155/273/343, 88, 91/95, 91/95/96, 92, 93, 96, 96/273, 96/312/343, 120, 120/299/305, 151, 158, 158/162/273, 162, 162/273, 162/343, 166, 178, 180, 181, 206, 217, 271, 273, 273/343, 282, 282/365, 293/391, 299/300, 299/300/305/365, 300, 301, 305, 305/365, 314, 333, 336, 337, 343, 345, 363, 365, 370, 389, 393, 394, 396/398, 397 및 398로부터 선택된 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열번호 58을 기준으로 넘버링된다. 일부 실시양태에서, 재조합 알파-갈락토시다제 A는 서열번호 58에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 이상의 서열 동일성을 가지는 폴리펩타이드 서열 또는 이의 기능적 단편을 포함하고, 여기서, 상기 재조합 알파-갈락토시다제 A는 7L, 7L/48D/68E, 7L/48D/68E/120H/282N/299R, 7L/48D/130E/282N, 7L/48D/180G, 7L/68E/130E/282N/365V, 7L/68E/180G, 7L/88A/120H/305G/365V, 7L/120H, 7L/130E, 7L/282N, 7L/305G, 7L/305G/365V, 7L/365V, 39V, 47D, 47D/87K/95E/96L/158R/162H, 47D/95E, 47D/273P, 47D/343G, 47V, 48D, 48D/68E, 48D/180G/282N, 48D/282N, 48D/282N/305G, 67T/180G, 68E, 68E/299R/300I, 71P, 87K/91Q/95E/96L/158A/162K, 87K/91Q/95E/96L/206S/343G, 87K/96I/155N/273P/343G, 88A, 91Q/95E, 91Q/95E/96L, 92F, 92T, 93I, 96L, 96L/273P, 96L/312Q/343G, 120H, 120H/299R/305G, 151L, 158A, 158A/162K/273G, 158R, 162H/343D, 162K, 162K/273P, 162S, 166K, 178G, 178S, 180G, 180L, 180T, 180V, 181A, 206K, 206S, 217K, 271R, 273P, 273P/343G, 282N, 282N/365V, 293P/391A, 299R/300I, 299R/300I/305G/365V, 300I, 301M, 305G, 305G/365V, 314A, 333F, 333G, 336V, 337R, 343D, 343G, 345A, 345Q, 363Q, 365A, 365Q, 365V, 370G, 389K, 393V, 394K, 396G/398T, 397A, 398A, 398P, 398S 및 398V로부터 선택된 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열번호 58을 기준으로 넘버링된다.
일부 실시양태에서, 재조합 알파-갈락토시다제 A는 서열번호 58에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 이상의 서열 동일성을 가지는 폴리펩타이드 서열 또는 이의 기능적 단편을 포함하고, 여기서, 상기 재조합 알파-갈락토시다제 A는 R7L, R7L/E48D/Q68E, R7L/E48D/Q68E/Y120H/D282N/Q299R, R7L/E48D/D130E/D282N, R7L/E48D/F180G, R7L/Q68E/D130E/D282N/F365V, R7L/Q68E/F180G, R7L/Q88A/Y120H/N305G/F365V, R7L/Y120H, R7L/D130E, R7L/D282N, R7L/N305G, R7L/N305G/F365V, R7L/F365V, E39V, T47D, T47D/R87K/S95E/K96L/L158R/R162H, T47D/S95E, T47D/S273P, T47D/K343G, T47V, E48D, E48D/Q68E, E48D/F180G/D282N, E48D/D282N, E48D/D282N/N305G, P67T/F180G, Q68E, Q68E/Q299R/L300I, S71P, R87K/N91Q/S95E/K96L/L158A/R162K, R87K/N91Q/S95E/K96L/A206S/K343G, R87K/K96I/H155N/S273P/K343G, Q88A, N91Q/S95E, N91Q/S95E/K96L, H92F, H92T, V93I, K96L, K96L/S273P, K96L/P312Q/K343G, Y120H, Y120H/Q299R/N305G, D151L, L158A, L158A/R162K/S273G, L158R, R162H/K343D, R162K, R162K/S273P, R162S, P166K, W178G, W178S, F180G, F180L, F180T, F180V, Q181A, A206K, A206S, R217K, A271R, S273P, S273P/K343G, D282N, D282N/F365V, L293P/Q391A, Q299R/L300I, Q299R/L300I/N305G/F365V, L300I, R301M, N305G, N305G/F365V, S314A, S333F, S333G, I336V, P337R, K343D, K343G, V345A, V345Q, L363Q, F365A, F365Q, F365V, S370G, T389K, S393V, L394K, D396G/L398T, L397A, L398A, L398P, L398S 및 L398V로부터 선택된 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열번호 58을 기준으로 넘버링된다.
본 발명은 또한 서열번호 158에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 이상의 서열 동일성을 가지는 폴리펩타이드 서열 또는 이의 기능적 단편을 포함하는 재조합 알파-갈락토시다제 A를 제공하고, 여기서, 상기 재조합 알파-갈락토시다제 A는 24/202, 39/47, 39/47/217, 39/151, 39/282/337/398, 39/337/343/398, 39/393/398, 47/130, 47/151, 47/343/345/393, 48, 48/68, 48/68/217/333/391/393, 48/68/333, 48/217, 48/333, 48/345/393, 48/393, 59/143, 68, 68/345, 130, 130/158, 130/158/393, 130/345/393, 143/271, 143/333, 143/387, 151, 151/158/217/343/345/393, 151/206/282/337/343/345/398, 151/282/393, 151/345/393/398, 151/393, 158, 158/393, 202, 206, 206/217, 217, 217/333, 217/337/345/398, 271, 282/393, 333, 333/345, 337/343/345/398, 343, 343/345/393/398, 393 및 393/398로부터 선택된 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열번호 158을 기준으로 넘버링된다. 일부 실시양태에서, 재조합 알파-갈락토시다제 A는 서열번호 158에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 이상의 서열 동일성을 가지는 폴리펩타이드 서열 또는 이의 기능적 단편을 포함하고, 여기서, 상기 재조합 알파-갈락토시다제 A는 24S/202N, 39V/47D, 39V/47V/217K, 39V/151L, 39V/282N/337R/398A, 39V/337R/343G/398A, 39V/393V/398A, 47V/130E, 47V/151L, 47V/343D/345Q/393V, 48D, 48D/68E, 48D/68E/217K/333F/391A/393V, 48D/68E/333F, 48D/217K, 48D/333F, 48D/333G, 48D/345Q/393V, 48D/393V, 59A/143S, 68E, 68E/345Q, 130E, 130E/158R, 130E/158R/393V, 130E/345Q/393V, 143S/271N, 143S/333N, 143S/387N, 151L, 151L/158R/217K/343G/345Q/393V, 151L/206S/282N/337R/343D/345Q/398A, 151L/282N/393V, 151L/345Q/393V/398A, 151L/393V, 158R, 158R/393V, 202N, 206S, 206S/217K, 217K, 217K/333F, 217K/333G, 217K/337R/345Q/398A, 271N, 282N/393V, 333F/345Q, 333G, 333N, 337R/343G/345Q/398A, 343D, 343D/345Q/393V/398A, 393V 및 393V/398A로부터 선택된 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열번호 158을 기준으로 넘버링된다. 일부 실시양태에서, 재조합 알파-갈락토시다제 A는 서열번호 158에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 이상의 서열 동일성을 가지는 폴리펩타이드 서열 또는 이의 기능적 단편을 포함하고, 여기서, 상기 재조합 알파-갈락토시다제 A는 D24S/D202N, E39V/T47D, E39V/T47V/R217K, E39V/D151L, E39V/D282N/P337R/L398A, E39V/P337R/K343G/L398A, E39V/S393V/L398A, T47V/D130E, T47V/D151L, T47V/K343D/V345Q/S393V, E48D, E48D/Q68E, E48D/Q68E/R217K/S333F/Q391A/S393V, E48D/Q68E/S333F, E48D/R217K, E48D/S333F, E48D/S333G, E48D/V345Q/S393V, E48D/S393V, C59A/C143S, Q68E, Q68E/V345Q, D130E, D130E/L158R, D130E/L158R/S393V, D130E/V345Q/S393V, C143S/A271N, C143S/S333N, C143S/E387N, D151L, D151L/L158R/R217K/K343G/V345Q/S393V, D151L/A206S/D282N/P337R/K343D/V345Q/L398A, D151L/D282N/S393V, D151L/V345Q/S393V/L398A, D151L/S393V, L158R, L158R/S393V, D202N, A206S, A206S/R217K, R217K, R217K/S333F, R217K/S333G, R217K/P337R/V345Q/L398A, A271N, D282N/S393V, S333F/V345Q, S333G, S333N, P337R/K343G/V345Q/L398A, K343D, K343D/V345Q/S393V/L398A, S393V 및 S393V/L398A로부터 선택된 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열번호 158을 기준으로 넘버링된다.
본 발명은 또한 서열번호 372에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 이상의 서열 동일성을 가지는 폴리펩타이드 서열 또는 이의 기능적 단편을 포함하는 재조합 알파-갈락토시다제 A를 제공하고, 여기서, 상기 재조합 알파-갈락토시다제 A는 10, 10/39/44/322, 10/39/92/206/217/271, 10/39/92/247, 10/39/92/247/271/316, 10/44, 10/44/47/92/247, 10/44/47/261/302/322/368, 10/44/92/316/322, 10/44/261/302/316, 10/44/302/337/368, 10/47/217/247/316/392, 10/47/217/322, 10/47/271, 10/92, 10/92/206/217/247, 10/92/206/247/316/322/392, 10/92/206/247/322/368, 10/92/217/261/302/337, 10/206/217/271, 10/206/247, 10/206/261/271/316, 10/261, 10/271/302, 10/302, 10/302/316, 10/302/322/337, 10/316/322, 10/337/392, 10/368, 39/44/92/162/247/302/316/322, 39/44/92/217/322, 39/44/92/247/271/302, 39/47/92/247/302/316/322, 39/47/217/247/368, 39/47/247, 39/92/247/302/316/337/368, 39/92/316/322, 39/247/271, 39/247/271/316, 39/322, 44/47/92/206/217/316/322, 44/47/92/247/261/271/316/337/368, 44/47/206/217/247/271/322, 44/47/247/322/368, 44/47/302/316/322, 44/92/206/247/368, 44/206/337, 44/247/261/302/316, 44/247/261/302/316/322, 47/92/247/271, 47/217/302, 47/247, 47/247/271, 89/217/247/261/302/316, 92/217/271, 92/247, 92/247/271/322, 92/247/302/322/337, 92/271/337, 92/302, 92/316, 206/217/271/392, 217/247/316/322/337/368, 247, 247/271, 247/302, 271, 271/302/322, 271/316/322, 302/322/368 및 368로부터 선택된 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열번호 372를 기준으로 넘버링된다. 일부 실시양태에서, 재조합 알파-갈락토시다제 A는 서열번호 372에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 이상의 서열 동일성을 가지는 폴리펩타이드 서열 또는 이의 기능적 단편을 포함하고, 여기서, 상기 재조합 알파-갈락토시다제 A는 10P, 10P/39E/44R/322I, 10P/39E/92H/206A/217R/271A, 10P/39E/92H/247D, 10P/39E/92H/247D/271A/316D, 10P/44R, 10P/44R/47T/92H/247D, 10P/44R/47T/261G/302K/322I/368A, 10P/44R/92H/316D/322I, 10P/44R/261G/302K/316D, 10P/44R/302K/337P/368A, 10P/47T/217R/247D/316D/392T, 10P/47T/217R/322I, 10P/47T/271A, 10P/92H, 10P/92H/206A/217R/247D, 10P/92H/206A/247D/316D/322I/392T, 10P/92H/206A/247D/322I/368A, 10P/92H/217R/261G/302K/337P, 10P/206A/217R/271A, 10P/206A/247D, 10P/206A/261G/271A/316D, 10P/261G, 10P/271A/302K, 10P/302K, 10P/302K/316D, 10P/302K/322I/337P, 10P/316D/322I, 10P/337P/392T, 10P/368A, 39E/44R/92H/162M/247D/302K/316D/322I, 39E/44R/92H/217R/322I, 39E/44R/92H/247D/271A/302K, 39E/47T/92H/247D/302K/316D/322I, 39E/47T/217R/247D/368A, 39E/47T/247D, 39E/92H/247D/302K/316D/337P/368A, 39E/92H/316D/322I, 39E/247D/271A, 39E/247D/271A/316D, 39E/322I, 44R/47T/92H/206A/217R/316D/322I, 44R/47T/92H/247D/261G/271A/316D/337P/368A, 44R/47T/206A/217R/247D/271A/322I, 44R/47T/247D/322I/368A, 44R/47T/302K/316D/322I, 44R/92H/206A/247D/368A, 44R/206A/337P, 44R/247D/261G/302K/316D, 44R/247D/261G/302K/316D/322I, 47T/92H/247D/271A, 47T/217R/302K, 47T/247D, 47T/247D/271A, 89I/217R/247D/261G/302K/316D, 92H/217R/271A, 92H/247D, 92H/247D/271A/322I, 92H/247D/302K/322I/337P, 92H/271A/337P, 92H/302K, 92H/316D, 206A/217R/271A/392T, 217R/247D/316D/322I/337P/368A, 247D, 247D/271A, 247D/302K, 271A, 271A/302K/322I, 271A/316D/322I, 302K/322I/368A 및 368A로부터 선택된 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열번호 372를 기준으로 넘버링된다. 일부 실시양태에서, 재조합 알파-갈락토시다제 A는 서열번호 372에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 이상의 서열 동일성을 가지는 폴리펩타이드 서열 또는 이의 기능적 단편을 포함하고, 여기서, 상기 재조합 알파-갈락토시다제 A는 T10P, T10P/M39E/L44R/M322I, T10P/M39E/Y92H/K206A/F217R/H271A, T10P/M39E/Y92H/N247D, T10P/M39E/Y92H/N247D/H271A/L316D, T10P/L44R, T10P/L44R/S47T/Y92H/N247D, T10P/L44R/S47T/A261G/Q302K/M322I/W368A, T10P/L44R/Y92H/L316D/M322I, T10P/L44R/A261G/Q302K/L316D, T10P/L44R/Q302K/A337P/W368A, T10P/S47T/F217R/N247D/L316D/M392T, T10P/S47T/F217R/M322I, T10P/S47T/H271A, T10P/Y92H, T10P/Y92H/K206A/F217R/N247D, T10P/Y92H/K206A/N247D/L316D/M322I/M392T, T10P/Y92H/K206A/N247D/M322I/W368A, T10P/Y92H/F217R/A261G/Q302K/A337P, T10P/K206A/F217R/H271A, T10P/K206A/N247D, T10P/K206A/A261G/H271A/L316D, T10P/A261G, T10P/H271A/Q302K, T10P/Q302K, T10P/Q302K/L316D, T10P/Q302K/M322I/A337P, T10P/L316D/M322I, T10P/A337P/M392T, T10P/W368A, M39E/L44R/Y92H/R162M/N247D/Q302K/L316D/M322I, M39E/L44R/Y92H/F217R/M322I, M39E/L44R/Y92H/N247D/H271A/Q302K, M39E/S47T/Y92H/N247D/Q302K/L316D/M322I, M39E/S47T/F217R/N247D/W368A, M39E/S47T/N247D, M39E/Y92H/N247D/Q302K/L316D/A337P/W368A, M39E/Y92H/L316D/M322I, M39E/N247D/H271A, M39E/N247D/H271A/L316D, M39E/M322I, L44R/S47T/Y92H/K206A/F217R/L316D/M322I, L44R/S47T/Y92H/N247D/A261G/H271A/L316D/A337P/W368A, L44R/S47T/K206A/F217R/N247D/H271A/M322I, L44R/S47T/N247D/M322I/W368A, L44R/S47T/Q302K/L316D/M322I, L44R/Y92H/K206A/N247D/W368A, L44R/K206A/A337P, L44R/N247D/A261G/Q302K/L316D, L44R/N247D/A261G/Q302K/L316D/M322I, S47T/Y92H/N247D/H271A, S47T/F217R/Q302K, S47T/N247D, S47T/N247D/H271A, L89I/F217R/N247D/A261G/Q302K/L316D, Y92H/F217R/H271A, Y92H/N247D, Y92H/N247D/H271A/M322I, Y92H/N247D/Q302K/M322I/A337P, Y92H/H271A/A337P, Y92H/Q302K, Y92H/L316D, K206A/F217R/H271A/M392T, F217R/N247D/L316D/M322I/A337P/W368A, N247D, N247D/H271A, N247D/Q302K, H271A, H271A/Q302K/M322I, H271A/L316D/M322I, Q302K/M322I/W368A 및 W368A로부터 선택된 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열번호 372를 기준으로 넘버링된다.
본 발명은 또한 서열번호 374에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 이상의 서열 동일성을 가지는 폴리펩타이드 서열 또는 이의 기능적 단편을 포함하는 재조합 알파-갈락토시다제 A를 제공하고, 여기서, 상기 재조합 알파-갈락토시다제 A는 10/36/92/166/247/261/316/392, 10/39, 10/39/44/47/92/206/217, 10/39/44/47/316, 10/39/44/47/337, 10/39/44/92/166/261/316/322, 10/39/44/92/166/302/322, 10/39/44/92/166/392, 10/39/44/92/217/302/322, 10/39/44/92/302/322, 10/39/44/166/261/271/316/322, 10/39/44/392, 10/39/47/92/337, 10/39/92/131/166/271/316/322, 10/39/92/166/217/247/271, 10/39/92/217/316, 10/44/47/166/261/271, 10/44/47/166/271/322/368, 10/44/47/217/271/316/322, 10/44/92, 10/44/92/217/247/271/302/316/392, 10/44/166/302, 10/44/206/316/322, 10/47/92/166/271/316/337, 10/47/92/271/302, 10/47/92/316/322/392, 10/47/166/271, 10/47/166/316, 10/92/166, 10/92/166/217/247/261/271, 10/92/166/261/271/392, 10/92/166/261/316/322/337, 10/92/166/337/368, 10/92/302/337, 10/92/316/322, 10/206, 10/206/247/261, 10/217/322, 10/261, 10/261/337/392, 10/316/392, 10/368, 39/44/47/92/166/206/392, 39/44/47/92/206/247/261, 39/44/47/92/206/392, 39/44/47/206/337/368/392, 39/44/92/166/247/261/302/337, 39/44/166/271, 39/44/166/271/337/368/392, 39/47/92/316/322, 39/47/92/392, 39/47/166/217/261/392, 39/47/217/247/368, 39/47/247, 39/92/166/217/392, 39/92/261/302, 39/166/217/261/316/368, 39/322, 39/392, 44/47, 44/47/92/217/271, 44/47/92/217/316/322/392, 44/47/92/392, 44/47/166, 44/47/166/271, 44/47/247/271/392, 44/316/322/392, 44/337, 47/166/206/217/247/337, 47/166/217/271/337, 47/206, 47/217/247/261, 47/271, 52/217/302/316, 92/166/206/271/316, 92/166/217/261/271/392, 92/166/217/316/337/392, 92/166/247, 92/166/316, 92/206/322, 92/217, 92/217/271/337, 92/261/271, 92/271, 166/217/316/322/337, 166/247/271/316, 166/316/322/337, 206/217, 217/392, 247/316, 316/322/368 및 316/337/392로부터 선택된 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열번호 374를 기준으로 넘버링된다. 일부 실시양태에서, 재조합 알파-갈락토시다제 A는 서열번호 374에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 이상의 서열 동일성을 가지는 폴리펩타이드 서열 또는 이의 기능적 단편을 포함하고, 여기서, 상기 재조합 알파-갈락토시다제 A는 10T/36M/92Y/166S/247N/261A/316L/392M, 10T/39M, 10T/39M/44L/47S/92Y/206K/217F, 10T/39M/44L/47S/316L, 10T/39M/44L/47S/337A, 10T/39M/44L/92Y/166S/261A/316L/322M, 10T/39M/44L/92Y/166S/302Q/322M, 10T/39M/44L/92Y/166S/392M, 10T/39M/44L/92Y/217F/302Q/322M, 10T/39M/44L/92Y/302Q/322M, 10T/39M/44L/166S/261A/271H/316L/322M, 10T/39M/44L/392M, 10T/39M/47S/92Y/337A, 10T/39M/92Y/131G/166S/271H/316L/322M, 10T/39M/92Y/166S/217F/247N/271H, 10T/39M/92Y/217F/316L, 10T/44L/47S/166S/261A/271H, 10T/44L/47S/166S/271H/322M/368W, 10T/44L/47S/217F/271H/316L/322M, 10T/44L/92Y, 10T/44L/92Y/217F/247N/271H/302Q/316L/392M, 10T/44L/166S/302Q, 10T/44L/206K/316L/322M, 10T/47S/92Y/166S/271H/316L/337A, 10T/47S/92Y/271H/302Q, 10T/47S/92Y/316L/322M/392M, 10T/47S/166S/271H, 10T/47S/166S/316L, 10T/92Y/166S, 10T/92Y/166S/217F/247N/261A/271H, 10T/92Y/166S/261A/271H/392M, 10T/92Y/166S/261A/316L/322M/337A, 10T/92Y/166S/337A/368W, 10T/92Y/302Q/337A, 10T/92Y/316L/322M, 10T/206K, 10T/206K/247N/261A, 10T/217F/322M, 10T/261A, 10T/261A/337A/392M, 10T/316L/392M, 10T/368W, 39M/44L/47S/92Y/166S/206K/392M, 39M/44L/47S/92Y/206K/247N/261A, 39M/44L/47S/92Y/206K/392M, 39M/44L/47S/206K/337A/368W/392M, 39M/44L/92Y/166S/247N/261A/302Q/337A, 39M/44L/166S/271H, 39M/44L/166S/271H/337A/368W/392M, 39M/47S/92Y/316L/322M, 39M/47S/92Y/392M, 39M/47S/166S/217F/261A/392M, 39M/47S/217F/247N/368W, 39M/47S/247N, 39M/92Y/166S/217F/392M, 39M/92Y/261A/302Q, 39M/166S/217F/261A/316L/368W, 39M/322M, 39M/392M, 44L/47S, 44L/47S/92Y/217F/271H, 44L/47S/92Y/217F/316L/322M/392M, 44L/47S/92Y/392M, 44L/47S/166S, 44L/47S/166S/271H, 44L/47S/247N/271H/392M, 44L/316L/322M/392M, 44L/337A, 47S/166S/206K/217F/247N/337A, 47S/166S/217F/271H/337A, 47S/206K, 47S/217F/247N/261A, 47S/271H, 52N/217F/302Q/316L, 92Y/166S/206K/271H/316L, 92Y/166S/217F/261A/271H/392M, 92Y/166S/217F/316L/337A/392M, 92Y/166S/247N, 92Y/166S/316L, 92Y/206K/322M, 92Y/217F, 92Y/217F/271H/337A, 92Y/261A/271H, 92Y/271H, 166S/217F/316L/322M/337A, 166S/247N/271H/316L, 166S/316L/322M/337A, 206K/217F, 217F/392M, 247N/316L, 316L/322M/368W 및 316L/337A/392M으로부터 선택된 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열번호 374를 기준으로 넘버링된다. 일부 실시양태에서, 재조합 알파-갈락토시다제 A는 서열번호 374에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 이상의 서열 동일성을 가지는 폴리펩타이드 서열 또는 이의 기능적 단편을 포함하고, 여기서, 상기 재조합 알파-갈락토시다제 A는 P10T/K36M/H92Y/P166S/D247N/G261A/D316L/T392M, P10T/E39M, P10T/E39M/R44L/T47S/H92Y/A206K/R217F, P10T/E39M/R44L/T47S/D316L, P10T/E39M/R44L/T47S/P337A, P10T/E39M/R44L/H92Y/P166S/G261A/D316L/I322M, P10T/E39M/R44L/H92Y/P166S/K302Q/I322M, P10T/E39M/R44L/H92Y/P166S/T392M, P10T/E39M/R44L/H92Y/R217F/K302Q/I322M, P10T/E39M/R44L/H92Y/K302Q/I322M, P10T/E39M/R44L/P166S/G261A/A271H/D316L/I322M, P10T/E39M/R44L/T392M, P10T/E39M/T47S/H92Y/P337A, P10T/E39M/H92Y/W131G/P166S/A271H/D316L/I322M, P10T/E39M/H92Y/P166S/R217F/D247N/A271H, P10T/E39M/H92Y/R217F/D316L, P10T/R44L/T47S/P166S/G261A/A271H, P10T/R44L/T47S/P166S/A271H/I322M/A368W, P10T/R44L/T47S/R217F/A271H/D316L/I322M, P10T/R44L/H92Y, P10T/R44L/H92Y/R217F/D247N/A271H/K302Q/D316L/T392M, P10T/R44L/P166S/K302Q, P10T/R44L/A206K/D316L/I322M, P10T/T47S/H92Y/P166S/A271H/D316L/P337A, P10T/T47S/H92Y/A271H/K302Q, P10T/T47S/H92Y/D316L/I322M/T392M, P10T/T47S/P166S/A271H, P10T/T47S/P166S/D316L, P10T/H92Y/P166S, P10T/H92Y/P166S/R217F/D247N/G261A/A271H, P10T/H92Y/P166S/G261A/A271H/T392M, P10T/H92Y/P166S/G261A/D316L/I322M/P337A, P10T/H92Y/P166S/P337A/A368W, P10T/H92Y/K302Q/P337A, P10T/H92Y/D316L/I322M, P10T/A206K, P10T/A206K/D247N/G261A, P10T/R217F/I322M, P10T/G261A, P10T/G261A/P337A/T392M, P10T/D316L/T392M, P10T/A368W, E39M/R44L/T47S/H92Y/P166S/A206K/T392M, E39M/R44L/T47S/H92Y/A206K/D247N/G261A, E39M/R44L/T47S/H92Y/A206K/T392M, E39M/R44L/T47S/A206K/P337A/A368W/T392M, E39M/R44L/H92Y/P166S/D247N/G261A/K302Q/P337A, E39M/R44L/P166S/A271H, E39M/R44L/P166S/A271H/P337A/A368W/T392M, E39M/T47S/H92Y/D316L/I322M, E39M/T47S/H92Y/T392M, E39M/T47S/P166S/R217F/G261A/T392M, E39M/T47S/R217F/D247N/A368W, E39M/T47S/D247N, E39M/H92Y/P166S/R217F/T392M, E39M/H92Y/G261A/K302Q, E39M/P166S/R217F/G261A/D316L/A368W, E39M/I322M, E39M/T392M, R44L/T47S, R44L/T47S/H92Y/R217F/A271H, R44L/T47S/H92Y/R217F/D316L/I322M/T392M, R44L/T47S/H92Y/T392M, R44L/T47S/P166S, R44L/T47S/P166S/A271H, R44L/T47S/D247N/A271H/T392M, R44L/D316L/I322M/T392M, R44L/P337A, T47S/P166S/A206K/R217F/D247N/P337A, T47S/P166S/R217F/A271H/P337A, T47S/A206K, T47S/R217F/D247N/G261A, T47S/A271H, D52N/R217F/K302Q/D316L, H92Y/P166S/A206K/A271H/D316L, H92Y/P166S/R217F/G261A/A271H/T392M, H92Y/P166S/R217F/D316L/P337A/T392M, H92Y/P166S/D247N, H92Y/P166S/D316L, H92Y/A206K/I322M, H92Y/R217F, H92Y/R217F/A271H/P337A, H92Y/G261A/A271H, H92Y/A271H, P166S/R217F/D316L/I322M/P337A, P166S/D247N/A271H/D316L, P166S/D316L/I322M/P337A, A206K/R217F, R217F/T392M, D247N/D316L, D316L/I322M/A368W 및 D316L/P337A/T392M으로부터 선택된 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열번호 374를 기준으로 넘버링된다.
본 발명은 또한 서열번호 704에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 이상의 서열 동일성을 가지는 폴리펩타이드 서열 또는 이의 기능적 단편을 포함하는 재조합 알파-갈락토시다제 A를 제공하고, 여기서, 상기 재조합 알파-갈락토시다제 A는 2, 4, 5, 24/59, 24/143/144, 24/143/202/333, 24/143/202/352/390/391, 24/143/333/352/387/390/391, 24/143/390/391, 24/202, 24/202/271, 24/202/333/352, 24/271/352, 24/352/387/390/391, 24/387/391, 31, 40, 59, 59/143, 59/143/202, 59/143/202/271/333, 59/143/271, 59/143/333, 59/202, 59/202/333, 59/271/387/390, 73, 76, 80, 83, 84, 91/215/361, 122, 123, 143, 143/202, 143/271, 143/271/352/390, 143/333, 143/333/387/390, 143/387/391, 147, 155, 164, 165, 179, 186, 202, 202/333, 210, 215/218, 218, 218/361, 218/361/398, 218/398, 246, 254/398, 271, 271/333, 271/333/390/391, 271/333/391, 271/352/391, 273, 275, 277, 278, 280, 281, 283, 284, 287, 300, 303, 304, 325, 331, 332, 333/352, 333/390/391, 333/391, 334, 335, 336, 338, 339, 340, 341, 343, 359, 360, 361, 362, 367, 369, 371, 373, 375, 377, 382, 382/398, 385, 387/391, 390 및 398로부터 선택된 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열번호 704를 기준으로 넘버링된다. 일부 실시양태에서, 재조합 알파-갈락토시다제 A는 서열번호 704에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 이상의 서열 동일성을 가지는 폴리펩타이드 서열 또는 이의 기능적 단편을 포함하고, 여기서, 상기 재조합 알파-갈락토시다제 A는 2S, 4L, 5M, 5V, 24S/59A, 24S/143S/144N, 24S/143S/202N/333N, 24S/143S/202N/352N/390N/391N, 24S/143S/333N/352N/387N/390T/391N, 24S/143S/390T/391N, 24S/202N, 24S/202N/271N, 24S/202N/333N/352N, 24S/271N/352N, 24S/352N/387N/390N/391N, 24S/387N/391N, 31F, 31H, 31L, 31T, 31W, 40Q, 59A, 59A/143S, 59A/143S/271N, 59A/202N, 59T, 59T/143S/202N, 59T/143S/333N, 59T/202N/333N, 59V/143S/202N/271N/333N, 59V/271N/387N/390T, 73A, 76A, 76F, 76M, 76S, 80T, 83R, 83S, 84G, 84K, 84R, 91S/215S/361T, 122E, 122N, 122S, 123Q, 123R, 123S, 123T, 143S, 143S/202N, 143S/271N, 143S/271N/352N/390N, 143S/333N, 143S/333N/387N/390T, 143S/387N/391N, 147L, 147S, 155A, 155D, 155F, 155L, 155R, 155T, 164E, 165I, 179H, 179L, 179R, 179W, 186E, 186F, 186M, 186P, 186R, 186S, 186Y, 202N, 202N/333N, 210I, 215S/218Y, 218Y, 218Y/361T, 218Y/361T/398F, 218Y/398F, 246Y, 254T/398F, 271N, 271N/333N, 271N/333N/390N/391N, 271N/333N/391N, 271N/352N/391N, 273L, 275A, 275G, 277Q, 277V, 278N, 278R, 278S, 280G, 281I, 281M, 283L, 283P, 283T, 283V, 284A, 284E, 284G, 284L, 284M, 284R, 284S, 287R, 300F, 303A, 303C, 303W, 304T, 304V, 304W, 325A, 331M, 332G, 332H, 333N/352N, 333N/390N/391N, 333N/390S/391N, 333N/391N, 334C, 334V, 335A, 335L, 336F, 336G, 336S, 336T, 338L, 339G, 339N, 339Q, 339V, 340H, 340I, 340K, 340M, 340P, 340W, 341F, 341M, 343L, 343R, 343S, 343W, 359F, 359L, 359R, 360H, 360V, 361T, 361V, 362H, 367A, 367D, 367L, 367M, 369D, 371G, 373L, 373S, 375L, 375Q, 377Q, 382I, 382I/398F, 385R, 387N/391N, 390S 및 398F로부터 선택된 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열번호 704를 기준으로 넘버링된다. 일부 실시양태에서, 재조합 알파-갈락토시다제 A는 서열번호 704에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 이상의 서열 동일성을 가지는 폴리펩타이드 서열 또는 이의 기능적 단편을 포함하고, 여기서, 상기 재조합 알파-갈락토시다제 A는 D2S, G4L, L5M, L5V, D24S/C59A, D24S/C143S/D144N, D24S/C143S/D202N/G333N, D24S/C143S/D202N/F352N/M390N/Q391N, D24S/C143S/G333N/F352N/E387N/M390T/Q391N, D24S/C143S/M390T/Q391N, D24S/D202N, D24S/D202N/A271N, D24S/D202N/G333N/F352N, D24S/A271N/F352N, D24S/F352N/E387N/M390N/Q391N, D24S/E387N/Q391N, S31F, S31H, S31L, S31T, S31W, E40Q, C59A, C59A/C143S, C59A/C143S/A271N, C59A/D202N, C59T, C59T/C143S/D202N, C59T/C143S/G333N, C59T/D202N/G333N, C59V/C143S/D202N/A271N/G333N, C59V/A271N/E387N/M390T, G73A, Q76A, Q76F, Q76M, Q76S, Q80T, P83R, P83S, H84G, H84K, H84R, N91S/T215S/R361T, D122E, D122N, D122S, I123Q, I123R, I123S, I123T, C143S, C143S/D202N, C143S/A271N, C143S/A271N/F352N/M390N, C143S/G333N, C143S/G333N/E387N/M390T, C143S/E387N/Q391N, E147L, E147S, H155A, H155D, H155F, H155L, H155R, H155T, G164E, R165I, P179H, P179L, P179R, P179W, T186E, T186F, T186M, T186P, T186R, T186S, T186Y, D202N, D202N/G333N, S210I, T215S/N218Y, N218Y, N218Y/R361T, N218Y/R361T/L398F, N218Y/L398F, W246Y, A254T/L398F, A271N, A271N/G333N, A271N/G333N/M390N/Q391N, A271N/G333N/Q391N, A271N/F352N/Q391N, S273L, Q275A, Q275G, K277Q, K277V, A278N, A278R, A278S, L280G, Q281I, Q281M, K283L, K283P, K283T, K283V, D284A, D284E, D284G, D284L, D284M, D284R, D284S, A287R, L300F, G303A, G303C, G303W, D304T, D304V, D304W, R325A, P331M, R332G, R332H, G333N/F352N, G333N/M390N/Q391N, G333N/M390S/Q391N, G333N/Q391N, Y334C, Y334V, T335A, T335L, I336F, I336G, I336S, I336T, V338L, A339G, A339N, A339Q, A339V, S340H, S340I, S340K, S340M, S340P, S340W, L341F, L341M, K343L, K343R, K343S, K343W, V359F, V359L, V359R, K360H, K360V, R361T, R361V, K362H, E367A, E367D, E367L, E367M, T369D, R371G, R373L, R373S, H375L, H375Q, N377Q, V382I, V382I/L398F, Q385R, E387N/Q391N, M390S 및 L398F로부터 선택된 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열번호 704를 기준으로 넘버링된다.
본 발명은 또한 서열번호 374에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 이상의 서열 동일성을 가지는 폴리펩타이드 서열 또는 이의 기능적 단편을 포함하는 재조합 알파-갈락토시다제 A를 제공하고, 여기서, 상기 재조합 알파-갈락토시다제 A는 10, 39, 44, 47, 92, 166, 206, 217, 247, 261, 271, 302, 316, 322, 337, 368 및 392로부터 선택된 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열번호 374를 기준으로 넘버링된다. 일부 실시양태에서, 재조합 알파-갈락토시다제 A는 서열번호 374에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 이상의 서열 동일성을 가지는 폴리펩타이드 서열 또는 이의 기능적 단편을 포함하고, 여기서, 상기 재조합 알파-갈락토시다제 A는 10A, 10C, 10D, 10E, 10F, 10G, 10H, 10I, 10K, 10L, 10M, 10N, 10Q, 10R, 10S, 10T, 10V, 10W, 10Y, 39A, 39C, 39D, 39F, 39G, 39H, 39I, 39K, 39L, 39M, 39N, 39P, 39Q, 39R, 39S, 39T, 39V, 39W, 39Y, 44A, 44C, 44D, 44E, 44F, 44G, 44H, 44I, 44K, 44L, 44N, 44P, 44Q, 44S, 44T, 44V, 44W, 44Y, 47A, 47C, 47D, 47E, 47F, 47G, 47H, 47I, 47K, 47L, 47M, 47N, 47P, 47Q, 47R, 47S, 47V, 47W, 47Y, 92A, 92C, 92D, 92E, 92F, 92G, 92I, 92K, 92L, 92M, 92N, 92P, 92Q, 92R, 92S, 92T, 92V, 92W, 92Y, 166A, 166C, 166D, 166E, 166F, 166G, 166H, 166I, 166K, 166L, 166M, 166N, 166Q, 166R, 166S, 166T, 166V, 166W, 166Y, 206C, 206D, 206E, 206F, 206G, 206H, 206I, 206K, 206L, 206M, 206N, 206P, 206Q, 206R, 206S, 206T, 206V, 206W, 206Y, 217A, 217C, 217D, 217E, 217F, 217G, 217H, 217I, 217K, 217L, 217M, 217N, 217P, 217Q, 217S, 217T, 217V, 217W, 217Y, 247A, 247C, 247E, 247F, 247G, 247H, 247I, 247K, 247L, 247M, 247N, 247P, 247Q, 247R, 247S, 247T, 247V, 247W, 247Y, 261A, 261C, 261D, 261E, 261F, 261H, 261I, 261K, 261L, 261M, 261N, 261P, 261Q, 261R, 261S, 261T, 261V, 261W, 261Y, 271C, 271D, 271E, 271F, 271G, 271H, 271I, 271K, 271L, 271M, 271N, 271P, 271Q, 271R, 271S, 271T, 271V, 271W, 271Y, 302A, 302C, 302D, 302E, 302F, 302G, 302H, 302I, 302L, 302M, 302N, 302P, 302Q, 302R, 302S, 302T, 302V, 302W, 302Y, 316A, 316C, 316E, 316F, 316G, 316H, 316I, 316K, 316L, 316M, 316N, 316P, 316Q, 316R, 316S, 316T, 316V, 316W, 316Y, 322A, 322C, 322D, 322E, 322F, 322G, 322H, 322K, 322L, 322M, 322N, 322P, 322Q, 322R, 322S, 322T, 322V, 322W, 322Y, 337A, 337C, 337D, 337E, 337F, 337G, 337H, 337I, 337K, 337L, 337M, 337N, 337Q, 337R, 337S, 337T, 337V, 337W, 337Y, 368C, 368D, 368E, 368F, 368G, 368H, 368I, 368K, 368L, 368M, 368N, 368P, 368Q, 368R, 368S, 368T, 368V, 368W, 368Y, 392A, 392C, 392D, 392E, 392F, 392G, 392H, 392I, 392K, 392L, 392M, 392N, 392P, 392Q, 392R, 392S, 392V, 392W 및 392Y로부터 선택된 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열번호 374를 기준으로 넘버링된다. 일부 실시양태에서, 재조합 알파-갈락토시다제 A는 서열번호 374에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 이상의 서열 동일성을 가지는 폴리펩타이드 서열 또는 이의 기능적 단편을 포함하고, 여기서, 상기 재조합 알파-갈락토시다제 A는 P10A, P10C, P10D, P10E, P10F, P10G, P10H, P10I, P10K, P10L, P10M, P10N, P10Q, P10R, P10S, P10T, P10V, P10W, P10Y, E39A, E39C, E39D, E39F, E39G, E39H, E39I, E39K, E39L, E39M, E39N, E39P, E39Q, E39R, E39S, E39T, E39V, E39W, E39Y, R44A, R44C, R44D, R44E, R44F, R44G, R44H, R44I, R44K, R44L, R44N, R44P, R44Q, R44S, R44T, R44V, R44W, R44Y, T47A, T47C, T47D, T47E, T47F, T47G, T47H, T47I, T47K, T47L, T47M, T47N, T47P, T47Q, T47R, T47S, T47V, T47W, T47Y, H92A, H92C, H92D, H92E, H92F, H92G, H92I, H92K, H92L, H92M, H92N, H92P, H92Q, H92R, H92S, H92T, H92V, H92W, H92Y, P166A, P166C, P166D, P166E, P166F, P166G, P166H, P166I, P166K, P166L, P166M, P166N, P166Q, P166R, P166S, P166T, P166V, P166W, P166Y, A206C, A206D, A206E, A206F, A206G, A206H, A206I, A206K, A206L, A206M, A206N, A206P, A206Q, A206R, A206S, A206T, A206V, A206W, A206Y, R217A, R217C, R217D, R217E, R217F, R217G, R217H, R217I, R217K, R217L, R217M, R217N, R217P, R217Q, R217S, R217T, R217V, R217W, R217Y, D247A, D247C, D247E, D247F, D247G, D247H, D247I, D247K, D247L, D247M, D247N, D247P, D247Q, D247R, D247S, D247T, D247V, D247W, D247Y, G261A, G261C, G261D, G261E, G261F, G261H, G261I, G261K, G261L, G261M, G261N, G261P, G261Q, G261R, G261S, G261T, G261V, G261W, G261Y, A271C, A271D, A271E, A271F, A271G, A271H, A271I, A271K, A271L, A271M, A271N, A271P, A271Q, A271R, A271S, A271T, A271V, A271W, A271Y, K302A, K302C, K302D, K302E, K302F, K302G, K302H, K302I, K302L, K302M, K302N, K302P, K302Q, K302R, K302S, K302T, K302V, K302W, K302Y, D316A, D316C, D316E, D316F, D316G, D316H, D316I, D316K, D316L, D316M, D316N, D316P, D316Q, D316R, D316S, D316T, D316V, D316W, D316Y, I322A, I322C, I322D, I322E, I322F, I322G, I322H, I322K, I322L, I322M, I322N, I322P, I322Q, I322R, I322S, I322T, I322V, I322W, I322Y, P337A, P337C, P337D, P337E, P337F, P337G, P337H, P337I, P337K, P337L, P337M, P337N, P337Q, P337R, P337S, P337T, P337V, P337W, P337Y, A368C, A368D, A368E, A368F, A368G, A368H, A368I, A368K, A368L, A368M, A368N, A368P, A368Q, A368R, A368S, A368T, A368V, A368W, A368Y, T392A, T392C, T392D, T392E, T392F, T392G, T392H, T392I, T392K, T392L, T392M, T392N, T392P, T392Q, T392R, T392S, T392V, T392W 및 T392Y로부터 선택된 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열번호 374를 기준으로 넘버링된다.
본 발명은 또한 서열번호 1022에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 이상의 서열 동일성을 가지는 폴리펩타이드 서열 또는 이의 기능적 단편을 포함하는 재조합 알파-갈락토시다제 A를 제공하고, 여기서, 상기 재조합 알파-갈락토시다제 A는 10, 10/392, 31, 31/39/44/166/302, 31/47, 31/283/284, 39, 39/44, 39/44/47, 39/44/47/261/283/284, 39/44/283, 39/44/339, 39/47/261, 39/92, 39/206, 39/284, 44, 44/284/302, 84, 84/92, 84/284/302/392, 84/316, 84/368/392, 92, 92/206/217, 92/206/275, 92/206/284, 92/206/302/368, 92/271, 92/271/277, 92/275/284, 92/283, 92/283/392, 92/284, 92/302, 92/316, 92/368, 155, 155/217, 155/368, 166, 166/283/284, 166/302, 206, 206/217, 206/334, 261, 261/283, 271, 271/368, 275, 283, 283/284, 283/392, 284, 302, 316, 334, 339, 368, 368/392 및 392로부터 선택된 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열번호 1022를 기준으로 넘버링된다. 일부 실시양태에서, 재조합 알파-갈락토시다제 A는 서열번호 1022에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 이상의 서열 동일성을 가지는 폴리펩타이드 서열 또는 이의 기능적 단편을 포함하고, 여기서, 상기 재조합 알파-갈락토시다제 A는 P10G, P10G/T392D, S31T, S31T/E39V/R44V/P166D/K302Y, S31T/T47R, S31T/K283L/D284A, E39L, E39L/H92V, E39L/A206E, E39L/D284S, E39V/R44V, E39V/R44V/T47R, E39V/R44V/T47R/G261S/K283L/D284A, E39V/R44V/K283T, E39V/R44V/A339N, E39V/T47R/G261S, R44V, R44V/D284E/K302Y, H84K, H84K/H92V, H84K/D284S/K302L/T392A, H84K/D316H, H84K/A368E/T392A, H92Q, H92T, H92T/A206E/R217N, H92T/A206E/K302T/A368E, H92T/A271K, H92T/A271K/K277R, H92T/K283P, H92T/K283V/T392W, H92T/D284M, H92T/K302L, H92T/A368E, H92V, H92V/A206E/D284S, H92V/A206Y/Q275A, H92V/Q275A/D284S, H92V/D284S, H92V/K302L, H92V/D316H, H155F, H155F/R217I, H155F/A368E, P166D, P166D/K283L/D284A, P166D/K302Y, A206E, A206E/R217N, A206I, A206Q, A206T/Y334C, A206Y, G261S, G261S/K283L, A271K, A271K/A368E, Q275A, K283L, K283P/T392W, K283T, K283T/D284E, D284E, D284M, D284S, K302L, K302Y, D316H, Y334C, A339N, A368E, A368E/T392W, T392A, T392D 및 T392W로부터 선택된 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열번호 1022를 기준으로 넘버링된다. 일부 실시양태에서, 재조합 알파-갈락토시다제 A는 서열번호 1022에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 이상의 서열 동일성을 가지는 폴리펩타이드 서열 또는 이의 기능적 단편을 포함하고, 여기서, 상기 재조합 알파-갈락토시다제 A는 10G, 10G/392D, 31T, 31T/39V/44V/166D/302Y, 31T/47R, 31T/283L/284A, 39L, 39L/92V, 39L/206E, 39L/284S, 39V/44V, 39V/44V/47R, 39V/44V/47R/261S/283L/284A, 39V/44V/283T, 39V/44V/339N, 39V/47R/261S, 44V, 44V/284E/302Y, 84K, 84K/92V, 84K/284S/302L/392A, 84K/316H, 84K/368E/392A, 92Q, 92T, 92T/206E/217N, 92T/206E/302T/368E, 92T/271K, 92T/271K/277R, 92T/283P, 92T/283V/392W, 92T/284M, 92T/302L, 92T/368E, 92V, 92V/206E/284S, 92V/206Y/275A, 92V/275A/284S, 92V/284S, 92V/302L, 92V/316H, 155F, 155F/217I, 155F/368E, 166D, 166D/283L/284A, 166D/302Y, 206E, 206E/217N, 206I, 206Q, 206T/334C, 206Y, 261S, 261S/283L, 271K, 271K/368E, 275A, 283L, 283P/392W, 283T, 283T/284E, 284E, 284M, 284S, 302L, 302Y, 316H, 334C, 339N, 368E, 368E/392W, 392A, 392D 및 392W로부터 선택된 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열번호 1022를 기준으로 넘버링된다.
일부 실시양태에서, 재조합 알파-갈락토시다제 A 폴리펩타이드 서열은 서열번호 4 내지 1864의 짝수 서열에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 이상의 서열 동일성을 가진다.
일부 실시양태에서, 조작된 GLA 폴리펩타이드는 본 발명에 의해 포괄되는 조작된 GLA 폴리펩타이드의 기능적 단편을 포함한다. 기능적 단편은 그의 기원인 조작된 GLA 폴리펩타이드(즉, 모 조작된 GLA)의 활성의 적어도 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 또는 99%를 가진다. 기능적 단편은 조작된 GLA의 모 서열의 적어도 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% 및 심지어 99%를 포함한다. 일부 실시양태에서, 기능적 단편은 5개 미만, 10개 미만, 15개 미만, 10개 미만, 25개 미만, 30개 미만, 35개 미만, 40개 미만, 45개 미만 및 50개 미만의 아미노산이 절단되어 있다.
조작된 폴리펩타이드를 코딩하는 폴리뉴클레오타이드, 발현 벡터 및 숙주 세포:
본 발명은 본원에 기재된 조작된 GLA 폴리펩타이드를 코딩하는 폴리뉴클레오타이드를 제공한다. 일부 실시양태에서, 상기 폴리뉴클레오타이드는 폴리펩타이드를 발현할 수 있는 재조합 폴리뉴클레오타이드를 생성하도록 유전자 발현을 조절하는 하나 이상의 이종 또는 동종 조절 서열에 작동 가능하게 연결된다. 조작된 GLA 폴리펩타이드를 코딩하는 이종 폴리뉴클레오타이드를 함유하는 발현 작제물을 적절한 숙주 세포 내로 도입하여, 상응하는 GLA 폴리펩타이드를 발현할 수 있다.
숙련된 당업자에게 자명할 바와 같이, 단백질 서열의 입수 가능성 및 다양한 아미노산들에 상응하는 코돈의 지식은 본 폴리펩타이드를 코딩할 수 있는 모든 폴리뉴클레오타이드들의 설명을 제공한다. 동일한 아미노산이 대안적 또는 동의어 코돈에 의해 코딩되는 유전 코드의 축퇴성은 매우 많은 수의 핵산들이 제조될 수 있게 하고, 이 핵산들 전부가 조작된 GLA 폴리펩타이드를 코딩한다. 따라서, 특정 아미노산 서열을 알고 있는, 당분야에서 숙련된 자는 단백질의 아미노산 서열을 변화시키지 않는 방식으로 하나 이상의 코돈의 서열을 단순히 변형시킴으로써 임의의 수의 상이한 핵산들을 제조할 수 있다. 이와 관련하여, 본 발명은 가능한 코돈 선택에 근거하여 조합을 선택함으로써 본원에 기재된 폴리펩타이드를 코딩하도록 만들어질 수 있는 폴리뉴클레오타이드의 각각의 모든 가능한 변이를 구체적으로 예상하고, 모든 이러한 변이는 표 2-1, 5-1, 6-1, 7-1, 8-1, 9-1, 11-1, 12-1 및/또는 13-1에서 제공된 변이체들을 포함하는, 본원에 기재된 임의의 폴리펩타이드에 대해 구체적으로 개시된 것으로서 간주되어야 한다.
다양한 실시양태에서, 코돈은 바람직하게는 단백질이 생성되는 숙주 세포에 알맞도록 선택된다. 예를 들어, 세균에서 사용되는 바람직한 코돈은 세균에서의 발현을 위해 사용된다. 결과적으로, 조작된 GLA 폴리펩타이드를 코딩하는 코돈 최적화된 폴리뉴클레오타이드는 전장 코딩 영역의 코돈 위치들 중 약 40%, 50%, 60%, 70%, 80% 또는 90% 초과의 위치들에서 바람직한 코돈을 함유한다. 일부 실시양태에서, 본 발명은 코돈이 인간 세포 또는 조직에서의 발현을 위해 최적화되어 있는 재조합 폴리뉴클레오타이드 서열을 제공한다.
일부 실시양태에서, 본 발명은 서열번호 1에 대해 적어도 약 85%, 약 86%, 약 87%, 약 88%, 약 89%, 약 90%, 약 91%, 약 92%, 약 93%, 약 94%, 약 95%, 약 96%, 약 97%, 약 98%, 약 99% 이상의 서열 동일성을 가진 재조합 폴리뉴클레오타이드 서열을 제공한다. 일부 실시양태에서, 본 발명은 서열번호 1에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 이상의 서열 동일성을 가진 재조합 폴리뉴클레오타이드 서열을 제공한다. 일부 실시양태에서, 본 발명은 서열번호 7에 대해 적어도 약 85%, 약 86%, 약 87%, 약 88%, 약 89%, 약 90%, 약 91%, 약 92%, 약 93%, 약 94%, 약 95%, 약 96%, 약 97%, 약 98%, 약 99% 이상의 서열 동일성을 가진 재조합 폴리뉴클레오타이드 서열을 제공한다. 일부 실시양태에서, 본 발명은 서열번호 7에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 이상의 서열 동일성을 가진 재조합 폴리뉴클레오타이드 서열을 제공한다. 일부 실시양태에서, 재조합 폴리뉴클레오타이드 서열은 서열번호 3 내지 1863의 홀수 서열에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 이상의 서열 동일성을 가진다.
일부 실시양태에서, 폴리뉴클레오타이드는 서열번호 8에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 이상의 서열 동일성을 가지는 폴리펩타이드 서열 또는 이의 기능적 단편을 포함하는 재조합 알파-갈락토시다제 A를 코딩하고, 여기서, 상기 재조합 알파-갈락토시다제 A는 44, 44/217, 44/217/316, 44/217/322, 44/217/322/337, 44/247, 44/247/302, 44/247/302/322, 44/247/322, 44/247/337, 44/247/362, 44/302, 44/337, 44/373, 217/322, 217/373, 247/322, 247/362, 302/322/362/373, 302/337, 316, 316/337, 322, 322/337, 362/373 및 373으로부터 선택된 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열번호 8을 기준으로 넘버링된다. 일부 실시양태에서, 폴리뉴클레오타이드는 서열번호 8에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 이상의 서열 동일성을 가지는 폴리펩타이드 서열 또는 이의 기능적 단편을 포함하는 재조합 알파-갈락토시다제 A를 코딩하고, 여기서, 상기 재조합 알파-갈락토시다제 A는 44L, 44L/217F, 44L/217F/316L, 44L/217F/322M, 44L/217F/322M/337A, 44L/247N, 44L/247N/302Q, 44L/247N/302Q/322M, 44L/247N/322M, 44L/247N/337A, 44L/247N/362K, 44L/302Q, 44L/337A, 44L/373R, 217F/322M, 217F/373R, 247N/322M, 247N/362K, 302Q/322M/362K/373R, 302Q/337A, 316L, 316L/337A, 322M, 322M/337A, 362K/373R 및 373R로부터 선택된 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열번호 8을 기준으로 넘버링된다. 일부 실시양태에서, 폴리뉴클레오타이드는 서열번호 8에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 이상의 서열 동일성을 가지는 폴리펩타이드 서열 또는 이의 기능적 단편을 포함하는 재조합 알파-갈락토시다제 A를 코딩하고, 여기서, 상기 재조합 알파-갈락토시다제 A는 R44L, R44L/R217F, R44L/R217F/D316L, R44L/R217F/I322M, R44L/R217F/I322M/P337A, R44L/D247N, R44L/D247N/K302Q, R44L/D247N/K302Q/I322M, R44L/D247N/I322M, R44L/D247N/P337A, R44L/D247N/Q362K, R44L/K302Q, R44L/P337A, R44L/K373R, R217F/I322M, R217F/K373R, D247N/I322M, D247N/Q362K, K302Q/I322M/Q362K/K373R, K302Q/P337A, D316L, D316L/P337A, I322M, I322M/P337A, Q362K/K373R 및 K373R로부터 선택된 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열번호 8을 기준으로 넘버링된다.
일부 실시양태에서, 폴리뉴클레오타이드는 서열번호 8에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 이상의 서열 동일성을 가지는 폴리펩타이드 서열 또는 이의 기능적 단편을 포함하는 재조합 알파-갈락토시다제 A를 코딩하고, 여기서, 상기 재조합 알파-갈락토시다제 A는 10/39/44/47/92/166/206/217/247/261/271/302/316/322/337/362/368/373/392, 44/217/316, 44/217/322/337, 166/362, 217/373 및 362/373으로부터 선택된 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열번호 8을 기준으로 넘버링된다. 일부 실시양태에서, 폴리뉴클레오타이드는 서열번호 8에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 이상의 서열 동일성을 가지는 폴리펩타이드 서열 또는 이의 기능적 단편을 포함하는 재조합 알파-갈락토시다제 A를 코딩하고, 여기서, 상기 재조합 알파-갈락토시다제 A는 10T/39M/44L/47S/92Y/166S/206K/217F/247N/261A/271H/302Q/316L/322M/337A/362K/368W/373R/392M, 44L/217F/316L, 44L/217F/322M/337A, 166A/362K, 217F/373R, 및 362K/373R로부터 선택된 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열번호 8을 기준으로 넘버링된다. 일부 실시양태에서, 폴리뉴클레오타이드는 서열번호 8에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 이상의 서열 동일성을 가지는 폴리펩타이드 서열 또는 이의 기능적 단편을 포함하는 재조합 알파-갈락토시다제 A를 코딩하고, 여기서, 상기 재조합 알파-갈락토시다제 A는 P10T/E39M/R44L/T47S/H92Y/P166S/A206K/R217F/D247N/G261A/A271H/K302Q/D316L/I322M/P337A/Q362K/A368W/K373R/T392M, R44L/R217F/D316L, R44L/R217F/I322M/P337A, P166A/Q362K, R217F/K373R, 및 Q362K/K373R로부터 선택된 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열번호 8을 기준으로 넘버링된다.
일부 실시양태에서, 본 발명은 서열번호 57에 대해 적어도 약 85%, 약 86%, 약 87%, 약 88%, 약 89%, 약 90%, 약 91%, 약 92%, 약 93%, 약 94%, 약 95%, 약 96%, 약 97%, 약 98%, 약 99% 이상의 서열 동일성을 가지는 재조합 폴리뉴클레오타이드 서열을 제공한다. 일부 실시양태에서, 본 발명은 서열번호 57에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 이상의 서열 동일성을 가지는 재조합 폴리뉴클레오타이드 서열을 제공한다. 일부 실시양태에서, 폴리뉴클레오타이드는 재조합 알파-갈락토시다제 A를 코딩하고, 여기서, 상기 재조합 알파-갈락토시다제 A는 서열번호 58에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 이상의 서열 동일성을 가지는 폴리펩타이드 서열 또는 이의 기능적 단편을 포함하고, 상기 재조합 알파-갈락토시다제 A는 7, 7/48/68, 7/48/68/120/282/299, 7/48/130/282, 7/48/180, 7/68/130/282/365, 7/68/180, 7/88/120/305/365, 7/120, 7/130, 7/282, 7/305, 7/305/365, 7/365, 39, 47, 47/87/95/96/158/162, 47/95, 47/273, 47/343, 48, 48/68, 48/180/282, 48/282, 48/282/305, 67/180, 68, 68/299/300, 71, 87/91/95/96/158/162, 87/91/95/96/206/343, 87/96/155/273/343, 88, 91/95, 91/95/96, 92, 93, 96, 96/273, 96/312/343, 120, 120/299/305, 151, 158, 158/162/273, 162, 162/273, 162/343, 166, 178, 180, 181, 206, 217, 271, 273, 273/343, 282, 282/365, 293/391, 299/300, 299/300/305/365, 300, 301, 305, 305/365, 314, 333, 336, 337, 343, 345, 363, 365, 370, 389, 393, 394, 396/398, 397 및 398로부터 선택된 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열번호 58을 기준으로 넘버링된다. 일부 실시양태에서, 재조합 알파-갈락토시다제 A는 서열번호 58에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 이상의 서열 동일성을 가지는 폴리펩타이드 서열 또는 이의 기능적 단편을 포함하고, 여기서, 상기 재조합 알파-갈락토시다제 A는 7L, 7L/48D/68E, 7L/48D/68E/120H/282N/299R, 7L/48D/130E/282N, 7L/48D/180G, 7L/68E/130E/282N/365V, 7L/68E/180G, 7L/88A/120H/305G/365V, 7L/120H, 7L/130E, 7L/282N, 7L/305G, 7L/305G/365V, 7L/365V, 39V, 47D, 47D/87K/95E/96L/158R/162H, 47D/95E, 47D/273P, 47D/343G, 47V, 48D, 48D/68E, 48D/180G/282N, 48D/282N, 48D/282N/305G, 67T/180G, 68E, 68E/299R/300I, 71P, 87K/91Q/95E/96L/158A/162K, 87K/91Q/95E/96L/206S/343G, 87K/96I/155N/273P/343G, 88A, 91Q/95E, 91Q/95E/96L, 92F, 92T, 93I, 96L, 96L/273P, 96L/312Q/343G, 120H, 120H/299R/305G, 151L, 158A, 158A/162K/273G, 158R, 162H/343D, 162K, 162K/273P, 162S, 166K, 178G, 178S, 180G, 180L, 180T, 180V, 181A, 206K, 206S, 217K, 271R, 273P, 273P/343G, 282N, 282N/365V, 293P/391A, 299R/300I, 299R/300I/305G/365V, 300I, 301M, 305G, 305G/365V, 314A, 333F, 333G, 336V, 337R, 343D, 343G, 345A, 345Q, 363Q, 365A, 365Q, 365V, 370G, 389K, 393V, 394K, 396G/398T, 397A, 398A, 398P, 398S 및 398V로부터 선택된 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열번호 58을 기준으로 넘버링된다. 일부 실시양태에서, 재조합 알파-갈락토시다제 A는 서열번호 58에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 이상의 서열 동일성을 가지는 폴리펩타이드 서열 또는 이의 기능적 단편을 포함하고, 여기서, 상기 재조합 알파-갈락토시다제 A는 R7L, R7L/E48D/Q68E, R7L/E48D/Q68E/Y120H/D282N/Q299R, R7L/E48D/D130E/D282N, R7L/E48D/F180G, R7L/Q68E/D130E/D282N/F365V, R7L/Q68E/F180G, R7L/Q88A/Y120H/N305G/F365V, R7L/Y120H, R7L/D130E, R7L/D282N, R7L/N305G, R7L/N305G/F365V, R7L/F365V, E39V, T47D, T47D/R87K/S95E/K96L/L158R/R162H, T47D/S95E, T47D/S273P, T47D/K343G, T47V, E48D, E48D/Q68E, E48D/F180G/D282N, E48D/D282N, E48D/D282N/N305G, P67T/F180G, Q68E, Q68E/Q299R/L300I, S71P, R87K/N91Q/S95E/K96L/L158A/R162K, R87K/N91Q/S95E/K96L/A206S/K343G, R87K/K96I/H155N/S273P/K343G, Q88A, N91Q/S95E, N91Q/S95E/K96L, H92F, H92T, V93I, K96L, K96L/S273P, K96L/P312Q/K343G, Y120H, Y120H/Q299R/N305G, D151L, L158A, L158A/R162K/S273G, L158R, R162H/K343D, R162K, R162K/S273P, R162S, P166K, W178G, W178S, F180G, F180L, F180T, F180V, Q181A, A206K, A206S, R217K, A271R, S273P, S273P/K343G, D282N, D282N/F365V, L293P/Q391A, Q299R/L300I, Q299R/L300I/N305G/F365V, L300I, R301M, N305G, N305G/F365V, S314A, S333F, S333G, I336V, P337R, K343D, K343G, V345A, V345Q, L363Q, F365A, F365Q, F365V, S370G, T389K, S393V, L394K, D396G/L398T, L397A, L398A, L398P, L398S 및 L398V로부터 선택된 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열번호 58을 기준으로 넘버링된다.
일부 실시양태에서, 본 발명은 서열번호 157에 대해 적어도 약 85%, 약 86%, 약 87%, 약 88%, 약 89%, 약 90%, 약 91%, 약 92%, 약 93%, 약 94%, 약 95%, 약 96%, 약 97%, 약 98%, 약 99% 이상의 서열 동일성을 가지는 재조합 폴리뉴클레오타이드 서열을 제공한다. 일부 실시양태에서, 본 발명은 서열번호 157에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 이상의 서열 동일성을 가지는 재조합 폴리뉴클레오타이드 서열을 제공한다. 일부 실시양태에서, 폴리뉴클레오타이드는 재조합 알파-갈락토시다제 A를 코딩하고, 여기서, 상기 재조합 알파-갈락토시다제 A는 서열번호 158에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 이상의 서열 동일성을 가지는 폴리뉴클레오타이드 서열 또는 이의 기능적 단편을 포함하고, 상기 재조합 알파-갈락토시다제 A는 24/202, 39/47, 39/47/217, 39/151, 39/282/337/398, 39/337/343/398, 39/393/398, 47/130, 47/151, 47/343/345/393, 48, 48/68, 48/68/217/333/391/393, 48/68/333, 48/217, 48/333, 48/345/393, 48/393, 59/143, 68, 68/345, 130, 130/158, 130/158/393, 130/345/393, 143/271, 143/333, 143/387, 151, 151/158/217/343/345/393, 151/206/282/337/343/345/398, 151/282/393, 151/345/393/398, 151/393, 158, 158/393, 202, 206, 206/217, 217, 217/333, 217/337/345/398, 271, 282/393, 333, 333/345, 337/343/345/398, 343, 343/345/393/398, 393 및 393/398로부터 선택된 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열번호 158을 기준으로 넘버링된다. 일부 실시양태에서, 재조합 알파-갈락토시다제 A는 서열번호 158에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 이상의 서열 동일성을 가지는 폴리펩타이드 서열 또는 이의 기능적 단편을 포함하고, 상기 재조합 알파-갈락토시다제 A는 24S/202N, 39V/47D, 39V/47V/217K, 39V/151L, 39V/282N/337R/398A, 39V/337R/343G/398A, 39V/393V/398A, 47V/130E, 47V/151L, 47V/343D/345Q/393V, 48D, 48D/68E, 48D/68E/217K/333F/391A/393V, 48D/68E/333F, 48D/217K, 48D/333F, 48D/333G, 48D/345Q/393V, 48D/393V, 59A/143S, 68E, 68E/345Q, 130E, 130E/158R, 130E/158R/393V, 130E/345Q/393V, 143S/271N, 143S/333N, 143S/387N, 151L, 151L/158R/217K/343G/345Q/393V, 151L/206S/282N/337R/343D/345Q/398A, 151L/282N/393V, 151L/345Q/393V/398A, 151L/393V, 158R, 158R/393V, 202N, 206S, 206S/217K, 217K, 217K/333F, 217K/333G, 217K/337R/345Q/398A, 271N, 282N/393V, 333F/345Q, 333G, 333N, 337R/343G/345Q/398A, 343D, 343D/345Q/393V/398A, 393V 및 393V/398A로부터 선택된 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열번호 158을 기준으로 넘버링된다. 일부 실시양태에서, 재조합 알파-갈락토시다제 A는 서열번호 8에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 이상의 서열 동일성을 가지는 폴리펩타이드 서열 또는 이의 기능적 단편을 포함하고, 여기서, 상기 재조합 알파-갈락토시다제 A는 D24S/D202N, E39V/T47D, E39V/T47V/R217K, E39V/D151L, E39V/D282N/P337R/L398A, E39V/P337R/K343G/L398A, E39V/S393V/L398A, T47V/D130E, T47V/D151L, T47V/K343D/V345Q/S393V, E48D, E48D/Q68E, E48D/Q68E/R217K/S333F/Q391A/S393V, E48D/Q68E/S333F, E48D/R217K, E48D/S333F, E48D/S333G, E48D/V345Q/S393V, E48D/S393V, C59A/C143S, Q68E, Q68E/V345Q, D130E, D130E/L158R, D130E/L158R/S393V, D130E/V345Q/S393V, C143S/A271N, C143S/S333N, C143S/E387N, D151L, D151L/L158R/R217K/K343G/V345Q/S393V, D151L/A206S/D282N/P337R/K343D/V345Q/L398A, D151L/D282N/S393V, D151L/V345Q/S393V/L398A, D151L/S393V, L158R, L158R/S393V, D202N, A206S, A206S/R217K, R217K, R217K/S333F, R217K/S333G, R217K/P337R/V345Q/L398A, A271N, D282N/S393V, S333F/V345Q, S333G, S333N, P337R/K343G/V345Q/L398A, K343D, K343D/V345Q/S393V/L398A, S393V 및 S393V/L398A로부터 선택된 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열번호 158을 기준으로 넘버링된다.
일부 실시양태에서, 본 발명은 서열번호 371에 대해 적어도 약 85%, 약 86%, 약 87%, 약 88%, 약 89%, 약 90%, 약 91%, 약 92%, 약 93%, 약 94%, 약 95%, 약 96%, 약 97%, 약 98%, 약 99% 이상의 서열 동일성을 가지는 재조합 폴리뉴클레오타이드 서열을 제공한다. 일부 실시양태에서, 본 발명은 서열번호 371에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 이상의 서열 동일성을 가지는 재조합 폴리뉴클레오타이드 서열을 제공한다. 일부 실시양태에서, 폴리뉴클레오타이드는 재조합 알파-갈락토시다제 A를 코딩하고, 여기서, 상기 재조합 알파-갈락토시다제 A는 서열번호 372에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 이상의 서열 동일성을 가지는 폴리펩타이드 서열 또는 이의 기능적 단편을 포함하고, 여기서, 상기 재조합 알파-갈락토시다제 A는 10, 10/39/44/322, 10/39/92/206/217/271, 10/39/92/247, 10/39/92/247/271/316, 10/44, 10/44/47/92/247, 10/44/47/261/302/322/368, 10/44/92/316/322, 10/44/261/302/316, 10/44/302/337/368, 10/47/217/247/316/392, 10/47/217/322, 10/47/271, 10/92, 10/92/206/217/247, 10/92/206/247/316/322/392, 10/92/206/247/322/368, 10/92/217/261/302/337, 10/206/217/271, 10/206/247, 10/206/261/271/316, 10/261, 10/271/302, 10/302, 10/302/316, 10/302/322/337, 10/316/322, 10/337/392, 10/368, 39/44/92/162/247/302/316/322, 39/44/92/217/322, 39/44/92/247/271/302, 39/47/92/247/302/316/322, 39/47/217/247/368, 39/47/247, 39/92/247/302/316/337/368, 39/92/316/322, 39/247/271, 39/247/271/316, 39/322, 44/47/92/206/217/316/322, 44/47/92/247/261/271/316/337/368, 44/47/206/217/247/271/322, 44/47/247/322/368, 44/47/302/316/322, 44/92/206/247/368, 44/206/337, 44/247/261/302/316, 44/247/261/302/316/322, 47/92/247/271, 47/217/302, 47/247, 47/247/271, 89/217/247/261/302/316, 92/217/271, 92/247, 92/247/271/322, 92/247/302/322/337, 92/271/337, 92/302, 92/316, 206/217/271/392, 217/247/316/322/337/368, 247, 247/271, 247/302, 271, 271/302/322, 271/316/322, 302/322/368 및 368로부터 선택된 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열번호 372를 기준으로 넘버링된다. 일부 실시양태에서, 재조합 알파-갈락토시다제 A는 서열번호 372에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 이상의 서열 동일성을 가지는 폴리펩타이드 서열 또는 이의 기능적 단편을 포함하고, 여기서, 상기 재조합 알파-갈락토시다제 A는 10P, 10P/39E/44R/322I, 10P/39E/92H/206A/217R/271A, 10P/39E/92H/247D, 10P/39E/92H/247D/271A/316D, 10P/44R, 10P/44R/47T/92H/247D, 10P/44R/47T/261G/302K/322I/368A, 10P/44R/92H/316D/322I, 10P/44R/261G/302K/316D, 10P/44R/302K/337P/368A, 10P/47T/217R/247D/316D/392T, 10P/47T/217R/322I, 10P/47T/271A, 10P/92H, 10P/92H/206A/217R/247D, 10P/92H/206A/247D/316D/322I/392T, 10P/92H/206A/247D/322I/368A, 10P/92H/217R/261G/302K/337P, 10P/206A/217R/271A, 10P/206A/247D, 10P/206A/261G/271A/316D, 10P/261G, 10P/271A/302K, 10P/302K, 10P/302K/316D, 10P/302K/322I/337P, 10P/316D/322I, 10P/337P/392T, 10P/368A, 39E/44R/92H/162M/247D/302K/316D/322I, 39E/44R/92H/217R/322I, 39E/44R/92H/247D/271A/302K, 39E/47T/92H/247D/302K/316D/322I, 39E/47T/217R/247D/368A, 39E/47T/247D, 39E/92H/247D/302K/316D/337P/368A, 39E/92H/316D/322I, 39E/247D/271A, 39E/247D/271A/316D, 39E/322I, 44R/47T/92H/206A/217R/316D/322I, 44R/47T/92H/247D/261G/271A/316D/337P/368A, 44R/47T/206A/217R/247D/271A/322I, 44R/47T/247D/322I/368A, 44R/47T/302K/316D/322I, 44R/92H/206A/247D/368A, 44R/206A/337P, 44R/247D/261G/302K/316D, 44R/247D/261G/302K/316D/322I, 47T/92H/247D/271A, 47T/217R/302K, 47T/247D, 47T/247D/271A, 89I/217R/247D/261G/302K/316D, 92H/217R/271A, 92H/247D, 92H/247D/271A/322I, 92H/247D/302K/322I/337P, 92H/271A/337P, 92H/302K, 92H/316D, 206A/217R/271A/392T, 217R/247D/316D/322I/337P/368A, 247D, 247D/271A, 247D/302K, 271A, 271A/302K/322I, 271A/316D/322I, 302K/322I/368A 및 368A로부터 선택된 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열번호 372를 기준으로 넘버링된다. 일부 실시양태에서, 재조합 알파-갈락토시다제 A는 서열번호 372에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 이상의 서열 동일성을 가지는 폴리펩타이드 서열 또는 이의 기능적 단편을 포함하고, 여기서, 상기 재조합 알파-갈락토시다제 A는 T10P, T10P/M39E/L44R/M322I, T10P/M39E/Y92H/K206A/F217R/H271A, T10P/M39E/Y92H/N247D, T10P/M39E/Y92H/N247D/H271A/L316D, T10P/L44R, T10P/L44R/S47T/Y92H/N247D, T10P/L44R/S47T/A261G/Q302K/M322I/W368A, T10P/L44R/Y92H/L316D/M322I, T10P/L44R/A261G/Q302K/L316D, T10P/L44R/Q302K/A337P/W368A, T10P/S47T/F217R/N247D/L316D/M392T, T10P/S47T/F217R/M322I, T10P/S47T/H271A, T10P/Y92H, T10P/Y92H/K206A/F217R/N247D, T10P/Y92H/K206A/N247D/L316D/M322I/M392T, T10P/Y92H/K206A/N247D/M322I/W368A, T10P/Y92H/F217R/A261G/Q302K/A337P, T10P/K206A/F217R/H271A, T10P/K206A/N247D, T10P/K206A/A261G/H271A/L316D, T10P/A261G, T10P/H271A/Q302K, T10P/Q302K, T10P/Q302K/L316D, T10P/Q302K/M322I/A337P, T10P/L316D/M322I, T10P/A337P/M392T, T10P/W368A, M39E/L44R/Y92H/R162M/N247D/Q302K/L316D/M322I, M39E/L44R/Y92H/F217R/M322I, M39E/L44R/Y92H/N247D/H271A/Q302K, M39E/S47T/Y92H/N247D/Q302K/L316D/M322I, M39E/S47T/F217R/N247D/W368A, M39E/S47T/N247D, M39E/Y92H/N247D/Q302K/L316D/A337P/W368A, M39E/Y92H/L316D/M322I, M39E/N247D/H271A, M39E/N247D/H271A/L316D, M39E/M322I, L44R/S47T/Y92H/K206A/F217R/L316D/M322I, L44R/S47T/Y92H/N247D/A261G/H271A/L316D/A337P/W368A, L44R/S47T/K206A/F217R/N247D/H271A/M322I, L44R/S47T/N247D/M322I/W368A, L44R/S47T/Q302K/L316D/M322I, L44R/Y92H/K206A/N247D/W368A, L44R/K206A/A337P, L44R/N247D/A261G/Q302K/L316D, L44R/N247D/A261G/Q302K/L316D/M322I, S47T/Y92H/N247D/H271A, S47T/F217R/Q302K, S47T/N247D, S47T/N247D/H271A, L89I/F217R/N247D/A261G/Q302K/L316D, Y92H/F217R/H271A, Y92H/N247D, Y92H/N247D/H271A/M322I, Y92H/N247D/Q302K/M322I/A337P, Y92H/H271A/A337P, Y92H/Q302K, Y92H/L316D, K206A/F217R/H271A/M392T, F217R/N247D/L316D/M322I/A337P/W368A, N247D, N247D/H271A, N247D/Q302K, H271A, H271A/Q302K/M322I, H271A/L316D/M322I, Q302K/M322I/W368A 및 W368A로부터 선택된 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열번호 372를 기준으로 넘버링된다.
일부 실시양태에서, 본 발명은 서열번호 373에 대해 적어도 약 85%, 약 86%, 약 87%, 약 88%, 약 89%, 약 90%, 약 91%, 약 92%, 약 93%, 약 94%, 약 95%, 약 96%, 약 97%, 약 98%, 약 99% 이상의 서열 동일성을 가지는 재조합 폴리뉴클레오타이드 서열을 제공한다. 일부 실시양태에서, 본 발명은 서열번호 373에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 이상의 서열 동일성을 가지는 재조합 폴리뉴클레오타이드 서열을 제공한다. 일부 실시양태에서, 폴리뉴클레오타이드는 재조합 알파-갈락토시다제 A를 코딩하고, 상기 재조합 알파-갈락토시다제 A는 서열번호 374에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 이상의 서열 동일성을 가지는 폴리펩타이드 서열 또는 이의 기능적 단편을 포함하고, 여기서, 상기 재조합 알파-갈락토시다제 A는 10/36/92/166/247/261/316/392, 10/39, 10/39/44/47/92/206/217, 10/39/44/47/316, 10/39/44/47/337, 10/39/44/92/166/261/316/322, 10/39/44/92/166/302/322, 10/39/44/92/166/392, 10/39/44/92/217/302/322, 10/39/44/92/302/322, 10/39/44/166/261/271/316/322, 10/39/44/392, 10/39/47/92/337, 10/39/92/131/166/271/316/322, 10/39/92/166/217/247/271, 10/39/92/217/316, 10/44/47/166/261/271, 10/44/47/166/271/322/368, 10/44/47/217/271/316/322, 10/44/92, 10/44/92/217/247/271/302/316/392, 10/44/166/302, 10/44/206/316/322, 10/47/92/166/271/316/337, 10/47/92/271/302, 10/47/92/316/322/392, 10/47/166/271, 10/47/166/316, 10/92/166, 10/92/166/217/247/261/271, 10/92/166/261/271/392, 10/92/166/261/316/322/337, 10/92/166/337/368, 10/92/302/337, 10/92/316/322, 10/206, 10/206/247/261, 10/217/322, 10/261, 10/261/337/392, 10/316/392, 10/368, 39/44/47/92/166/206/392, 39/44/47/92/206/247/261, 39/44/47/92/206/392, 39/44/47/206/337/368/392, 39/44/92/166/247/261/302/337, 39/44/166/271, 39/44/166/271/337/368/392, 39/47/92/316/322, 39/47/92/392, 39/47/166/217/261/392, 39/47/217/247/368, 39/47/247, 39/92/166/217/392, 39/92/261/302, 39/166/217/261/316/368, 39/322, 39/392, 44/47, 44/47/92/217/271, 44/47/92/217/316/322/392, 44/47/92/392, 44/47/166, 44/47/166/271, 44/47/247/271/392, 44/316/322/392, 44/337, 47/166/206/217/247/337, 47/166/217/271/337, 47/206, 47/217/247/261, 47/271, 52/217/302/316, 92/166/206/271/316, 92/166/217/261/271/392, 92/166/217/316/337/392, 92/166/247, 92/166/316, 92/206/322, 92/217, 92/217/271/337, 92/261/271, 92/271, 166/217/316/322/337, 166/247/271/316, 166/316/322/337, 206/217, 217/392, 247/316, 316/322/368 및 316/337/392로부터 선택된 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열번호 374를 기준으로 넘버링된다. 일부 실시양태에서, 재조합 알파-갈락토시다제 A는 서열번호 374에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 이상의 서열 동일성을 가지는 폴리펩타이드 서열 또는 이의 기능적 단편을 포함하고, 여기서, 상기 재조합 알파-갈락토시다제 A는 10T/36M/92Y/166S/247N/261A/316L/392M, 10T/39M, 10T/39M/44L/47S/92Y/206K/217F, 10T/39M/44L/47S/316L, 10T/39M/44L/47S/337A, 10T/39M/44L/92Y/166S/261A/316L/322M, 10T/39M/44L/92Y/166S/302Q/322M, 10T/39M/44L/92Y/166S/392M, 10T/39M/44L/92Y/217F/302Q/322M, 10T/39M/44L/92Y/302Q/322M, 10T/39M/44L/166S/261A/271H/316L/322M, 10T/39M/44L/392M, 10T/39M/47S/92Y/337A, 10T/39M/92Y/131G/166S/271H/316L/322M, 10T/39M/92Y/166S/217F/247N/271H, 10T/39M/92Y/217F/316L, 10T/44L/47S/166S/261A/271H, 10T/44L/47S/166S/271H/322M/368W, 10T/44L/47S/217F/271H/316L/322M, 10T/44L/92Y, 10T/44L/92Y/217F/247N/271H/302Q/316L/392M, 10T/44L/166S/302Q, 10T/44L/206K/316L/322M, 10T/47S/92Y/166S/271H/316L/337A, 10T/47S/92Y/271H/302Q, 10T/47S/92Y/316L/322M/392M, 10T/47S/166S/271H, 10T/47S/166S/316L, 10T/92Y/166S, 10T/92Y/166S/217F/247N/261A/271H, 10T/92Y/166S/261A/271H/392M, 10T/92Y/166S/261A/316L/322M/337A, 10T/92Y/166S/337A/368W, 10T/92Y/302Q/337A, 10T/92Y/316L/322M, 10T/206K, 10T/206K/247N/261A, 10T/217F/322M, 10T/261A, 10T/261A/337A/392M, 10T/316L/392M, 10T/368W, 39M/44L/47S/92Y/166S/206K/392M, 39M/44L/47S/92Y/206K/247N/261A, 39M/44L/47S/92Y/206K/392M, 39M/44L/47S/206K/337A/368W/392M, 39M/44L/92Y/166S/247N/261A/302Q/337A, 39M/44L/166S/271H, 39M/44L/166S/271H/337A/368W/392M, 39M/47S/92Y/316L/322M, 39M/47S/92Y/392M, 39M/47S/166S/217F/261A/392M, 39M/47S/217F/247N/368W, 39M/47S/247N, 39M/92Y/166S/217F/392M, 39M/92Y/261A/302Q, 39M/166S/217F/261A/316L/368W, 39M/322M, 39M/392M, 44L/47S, 44L/47S/92Y/217F/271H, 44L/47S/92Y/217F/316L/322M/392M, 44L/47S/92Y/392M, 44L/47S/166S, 44L/47S/166S/271H, 44L/47S/247N/271H/392M, 44L/316L/322M/392M, 44L/337A, 47S/166S/206K/217F/247N/337A, 47S/166S/217F/271H/337A, 47S/206K, 47S/217F/247N/261A, 47S/271H, 52N/217F/302Q/316L, 92Y/166S/206K/271H/316L, 92Y/166S/217F/261A/271H/392M, 92Y/166S/217F/316L/337A/392M, 92Y/166S/247N, 92Y/166S/316L, 92Y/206K/322M, 92Y/217F, 92Y/217F/271H/337A, 92Y/261A/271H, 92Y/271H, 166S/217F/316L/322M/337A, 166S/247N/271H/316L, 166S/316L/322M/337A, 206K/217F, 217F/392M, 247N/316L, 316L/322M/368W 및 316L/337A/392M으로부터 선택된 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열번호 374를 기준으로 넘버링된다. 일부 실시양태에서, 재조합 알파-갈락토시다제 A는 서열번호 374에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 이상의 서열 동일성을 가지는 폴리펩타이드 서열 또는 이의 기능적 단편을 포함하고, 여기서, 상기 재조합 알파-갈락토시다제 A는 P10T/K36M/H92Y/P166S/D247N/G261A/D316L/T392M, P10T/E39M, P10T/E39M/R44L/T47S/H92Y/A206K/R217F, P10T/E39M/R44L/T47S/D316L, P10T/E39M/R44L/T47S/P337A, P10T/E39M/R44L/H92Y/P166S/G261A/D316L/I322M, P10T/E39M/R44L/H92Y/P166S/K302Q/I322M, P10T/E39M/R44L/H92Y/P166S/T392M, P10T/E39M/R44L/H92Y/R217F/K302Q/I322M, P10T/E39M/R44L/H92Y/K302Q/I322M, P10T/E39M/R44L/P166S/G261A/A271H/D316L/I322M, P10T/E39M/R44L/T392M, P10T/E39M/T47S/H92Y/P337A, P10T/E39M/H92Y/W131G/P166S/A271H/D316L/I322M, P10T/E39M/H92Y/P166S/R217F/D247N/A271H, P10T/E39M/H92Y/R217F/D316L, P10T/R44L/T47S/P166S/G261A/A271H, P10T/R44L/T47S/P166S/A271H/I322M/A368W, P10T/R44L/T47S/R217F/A271H/D316L/I322M, P10T/R44L/H92Y, P10T/R44L/H92Y/R217F/D247N/A271H/K302Q/D316L/T392M, P10T/R44L/P166S/K302Q, P10T/R44L/A206K/D316L/I322M, P10T/T47S/H92Y/P166S/A271H/D316L/P337A, P10T/T47S/H92Y/A271H/K302Q, P10T/T47S/H92Y/D316L/I322M/T392M, P10T/T47S/P166S/A271H, P10T/T47S/P166S/D316L, P10T/H92Y/P166S, P10T/H92Y/P166S/R217F/D247N/G261A/A271H, P10T/H92Y/P166S/G261A/A271H/T392M, P10T/H92Y/P166S/G261A/D316L/I322M/P337A, P10T/H92Y/P166S/P337A/A368W, P10T/H92Y/K302Q/P337A, P10T/H92Y/D316L/I322M, P10T/A206K, P10T/A206K/D247N/G261A, P10T/R217F/I322M, P10T/G261A, P10T/G261A/P337A/T392M, P10T/D316L/T392M, P10T/A368W, E39M/R44L/T47S/H92Y/P166S/A206K/T392M, E39M/R44L/T47S/H92Y/A206K/D247N/G261A, E39M/R44L/T47S/H92Y/A206K/T392M, E39M/R44L/T47S/A206K/P337A/A368W/T392M, E39M/R44L/H92Y/P166S/D247N/G261A/K302Q/P337A, E39M/R44L/P166S/A271H, E39M/R44L/P166S/A271H/P337A/A368W/T392M, E39M/T47S/H92Y/D316L/I322M, E39M/T47S/H92Y/T392M, E39M/T47S/P166S/R217F/G261A/T392M, E39M/T47S/R217F/D247N/A368W, E39M/T47S/D247N, E39M/H92Y/P166S/R217F/T392M, E39M/H92Y/G261A/K302Q, E39M/P166S/R217F/G261A/D316L/A368W, E39M/I322M, E39M/T392M, R44L/T47S, R44L/T47S/H92Y/R217F/A271H, R44L/T47S/H92Y/R217F/D316L/I322M/T392M, R44L/T47S/H92Y/T392M, R44L/T47S/P166S, R44L/T47S/P166S/A271H, R44L/T47S/D247N/A271H/T392M, R44L/D316L/I322M/T392M, R44L/P337A, T47S/P166S/A206K/R217F/D247N/P337A, T47S/P166S/R217F/A271H/P337A, T47S/A206K, T47S/R217F/D247N/G261A, T47S/A271H, D52N/R217F/K302Q/D316L, H92Y/P166S/A206K/A271H/D316L, H92Y/P166S/R217F/G261A/A271H/T392M, H92Y/P166S/R217F/D316L/P337A/T392M, H92Y/P166S/D247N, H92Y/P166S/D316L, H92Y/A206K/I322M, H92Y/R217F, H92Y/R217F/A271H/P337A, H92Y/G261A/A271H, H92Y/A271H, P166S/R217F/D316L/I322M/P337A, P166S/D247N/A271H/D316L, P166S/D316L/I322M/P337A, A206K/R217F, R217F/T392M, D247N/D316L, D316L/I322M/A368W 및 D316L/P337A/T392M으로부터 선택된 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열번호 374를 기준으로 넘버링된다.
일부 실시양태에서, 폴리뉴클레오타이드는 서열번호 704에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 이상의 서열 동일성을 가지는 폴리펩타이드 서열 또는 이의 기능적 단편을 포함하는 재조합 알파-갈락토시다제 A를 코딩하고, 여기서, 상기 재조합 알파-갈락토시다제 A는 2, 4, 5, 24/59, 24/143/144, 24/143/202/333, 24/143/202/352/390/391, 24/143/333/352/387/390/391, 24/143/390/391, 24/202, 24/202/271, 24/202/333/352, 24/271/352, 24/352/387/390/391, 24/387/391, 31, 40, 59, 59/143, 59/143/202, 59/143/202/271/333, 59/143/271, 59/143/333, 59/202, 59/202/333, 59/271/387/390, 73, 76, 80, 83, 84, 91/215/361, 122, 123, 143, 143/202, 143/271, 143/271/352/390, 143/333, 143/333/387/390, 143/387/391, 147, 155, 164, 165, 179, 186, 202, 202/333, 210, 215/218, 218, 218/361, 218/361/398, 218/398, 246, 254/398, 271, 271/333, 271/333/390/391, 271/333/391, 271/352/391, 273, 275, 277, 278, 280, 281, 283, 284, 287, 300, 303, 304, 325, 331, 332, 333/352, 333/390/391, 333/391, 334, 335, 336, 338, 339, 340, 341, 343, 359, 360, 361, 362, 367, 369, 371, 373, 375, 377, 382, 382/398, 385, 387/391, 390 및 398로부터 선택된 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열번호 704를 기준으로 넘버링된다. 일부 실시양태에서, 재조합 알파-갈락토시다제 A는 서열번호 704에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 이상의 서열 동일성을 가지는 폴리펩타이드 서열 또는 이의 기능적 단편을 포함하고, 여기서, 상기 재조합 알파-갈락토시다제 A는 2S, 4L, 5M, 5V, 24S/59A, 24S/143S/144N, 24S/143S/202N/333N, 24S/143S/202N/352N/390N/391N, 24S/143S/333N/352N/387N/390T/391N, 24S/143S/390T/391N, 24S/202N, 24S/202N/271N, 24S/202N/333N/352N, 24S/271N/352N, 24S/352N/387N/390N/391N, 24S/387N/391N, 31F, 31H, 31L, 31T, 31W, 40Q, 59A, 59A/143S, 59A/143S/271N, 59A/202N, 59T, 59T/143S/202N, 59T/143S/333N, 59T/202N/333N, 59V/143S/202N/271N/333N, 59V/271N/387N/390T, 73A, 76A, 76F, 76M, 76S, 80T, 83R, 83S, 84G, 84K, 84R, 91S/215S/361T, 122E, 122N, 122S, 123Q, 123R, 123S, 123T, 143S, 143S/202N, 143S/271N, 143S/271N/352N/390N, 143S/333N, 143S/333N/387N/390T, 143S/387N/391N, 147L, 147S, 155A, 155D, 155F, 155L, 155R, 155T, 164E, 165I, 179H, 179L, 179R, 179W, 186E, 186F, 186M, 186P, 186R, 186S, 186Y, 202N, 202N/333N, 210I, 215S/218Y, 218Y, 218Y/361T, 218Y/361T/398F, 218Y/398F, 246Y, 254T/398F, 271N, 271N/333N, 271N/333N/390N/391N, 271N/333N/391N, 271N/352N/391N, 273L, 275A, 275G, 277Q, 277V, 278N, 278R, 278S, 280G, 281I, 281M, 283L, 283P, 283T, 283V, 284A, 284E, 284G, 284L, 284M, 284R, 284S, 287R, 300F, 303A, 303C, 303W, 304T, 304V, 304W, 325A, 331M, 332G, 332H, 333N/352N, 333N/390N/391N, 333N/390S/391N, 333N/391N, 334C, 334V, 335A, 335L, 336F, 336G, 336S, 336T, 338L, 339G, 339N, 339Q, 339V, 340H, 340I, 340K, 340M, 340P, 340W, 341F, 341M, 343L, 343R, 343S, 343W, 359F, 359L, 359R, 360H, 360V, 361T, 361V, 362H, 367A, 367D, 367L, 367M, 369D, 371G, 373L, 373S, 375L, 375Q, 377Q, 382I, 382I/398F, 385R, 387N/391N, 390S 및 398F로부터 선택된 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열번호 704를 기준으로 넘버링된다. 일부 실시양태에서, 재조합 알파-갈락토시다제 A는 서열번호 704에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 이상의 서열 동일성을 가지는 폴리펩타이드 서열 또는 이의 기능적 단편을 포함하고, 여기서, 상기 재조합 알파-갈락토시다제 A는 D2S, G4L, L5M, L5V, D24S/C59A, D24S/C143S/D144N, D24S/C143S/D202N/G333N, D24S/C143S/D202N/F352N/M390N/Q391N, D24S/C143S/G333N/F352N/E387N/M390T/Q391N, D24S/C143S/M390T/Q391N, D24S/D202N, D24S/D202N/A271N, D24S/D202N/G333N/F352N, D24S/A271N/F352N, D24S/F352N/E387N/M390N/Q391N, D24S/E387N/Q391N, S31F, S31H, S31L, S31T, S31W, E40Q, C59A, C59A/C143S, C59A/C143S/A271N, C59A/D202N, C59T, C59T/C143S/D202N, C59T/C143S/G333N, C59T/D202N/G333N, C59V/C143S/D202N/A271N/G333N, C59V/A271N/E387N/M390T, G73A, Q76A, Q76F, Q76M, Q76S, Q80T, P83R, P83S, H84G, H84K, H84R, N91S/T215S/R361T, D122E, D122N, D122S, I123Q, I123R, I123S, I123T, C143S, C143S/D202N, C143S/A271N, C143S/A271N/F352N/M390N, C143S/G333N, C143S/G333N/E387N/M390T, C143S/E387N/Q391N, E147L, E147S, H155A, H155D, H155F, H155L, H155R, H155T, G164E, R165I, P179H, P179L, P179R, P179W, T186E, T186F, T186M, T186P, T186R, T186S, T186Y, D202N, D202N/G333N, S210I, T215S/N218Y, N218Y, N218Y/R361T, N218Y/R361T/L398F, N218Y/L398F, W246Y, A254T/L398F, A271N, A271N/G333N, A271N/G333N/M390N/Q391N, A271N/G333N/Q391N, A271N/F352N/Q391N, S273L, Q275A, Q275G, K277Q, K277V, A278N, A278R, A278S, L280G, Q281I, Q281M, K283L, K283P, K283T, K283V, D284A, D284E, D284G, D284L, D284M, D284R, D284S, A287R, L300F, G303A, G303C, G303W, D304T, D304V, D304W, R325A, P331M, R332G, R332H, G333N/F352N, G333N/M390N/Q391N, G333N/M390S/Q391N, G333N/Q391N, Y334C, Y334V, T335A, T335L, I336F, I336G, I336S, I336T, V338L, A339G, A339N, A339Q, A339V, S340H, S340I, S340K, S340M, S340P, S340W, L341F, L341M, K343L, K343R, K343S, K343W, V359F, V359L, V359R, K360H, K360V, R361T, R361V, K362H, E367A, E367D, E367L, E367M, T369D, R371G, R373L, R373S, H375L, H375Q, N377Q, V382I, V382I/L398F, Q385R, E387N/Q391N, M390S 및 L398F로부터 선택된 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열번호 704를 기준으로 넘버링된다.
일부 실시양태에서, 폴리뉴클레오타이드는 서열번호 374에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 이상의 서열 동일성을 가지는 폴리펩타이드 서열 또는 이의 기능적 단편을 포함하는 재조합 알파-갈락토시다제 A를 코딩하고, 여기서, 상기 재조합 알파-갈락토시다제 A는 10, 39, 44, 47, 92, 166, 206, 217, 247, 261, 271, 302, 316, 322, 337, 368 및 392로부터 선택된 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열번호 374를 기준으로 넘버링된다. 일부 실시양태에서, 재조합 알파-갈락토시다제 A는 서열번호 374에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 이상의 서열 동일성을 가지는 폴리펩타이드 서열 또는 이의 기능적 단편을 포함하고, 여기서, 상기 재조합 알파-갈락토시다제 A는 10A, 10C, 10D, 10E, 10F, 10G, 10H, 10I, 10K, 10L, 10M, 10N, 10Q, 10R, 10S, 10T, 10V, 10W, 10Y, 39A, 39C, 39D, 39F, 39G, 39H, 39I, 39K, 39L, 39M, 39N, 39P, 39Q, 39R, 39S, 39T, 39V, 39W, 39Y, 44A, 44C, 44D, 44E, 44F, 44G, 44H, 44I, 44K, 44L, 44N, 44P, 44Q, 44S, 44T, 44V, 44W, 44Y, 47A, 47C, 47D, 47E, 47F, 47G, 47H, 47I, 47K, 47L, 47M, 47N, 47P, 47Q, 47R, 47S, 47V, 47W, 47Y, 92A, 92C, 92D, 92E, 92F, 92G, 92I, 92K, 92L, 92M, 92N, 92P, 92Q, 92R, 92S, 92T, 92V, 92W, 92Y, 166A, 166C, 166D, 166E, 166F, 166G, 166H, 166I, 166K, 166L, 166M, 166N, 166Q, 166R, 166S, 166T, 166V, 166W, 166Y, 206C, 206D, 206E, 206F, 206G, 206H, 206I, 206K, 206L, 206M, 206N, 206P, 206Q, 206R, 206S, 206T, 206V, 206W, 206Y, 217A, 217C, 217D, 217E, 217F, 217G, 217H, 217I, 217K, 217L, 217M, 217N, 217P, 217Q, 217S, 217T, 217V, 217W, 217Y, 247A, 247C, 247E, 247F, 247G, 247H, 247I, 247K, 247L, 247M, 247N, 247P, 247Q, 247R, 247S, 247T, 247V, 247W, 247Y, 261A, 261C, 261D, 261E, 261F, 261H, 261I, 261K, 261L, 261M, 261N, 261P, 261Q, 261R, 261S, 261T, 261V, 261W, 261Y, 271C, 271D, 271E, 271F, 271G, 271H, 271I, 271K, 271L, 271M, 271N, 271P, 271Q, 271R, 271S, 271T, 271V, 271W, 271Y, 302A, 302C, 302D, 302E, 302F, 302G, 302H, 302I, 302L, 302M, 302N, 302P, 302Q, 302R, 302S, 302T, 302V, 302W, 302Y, 316A, 316C, 316E, 316F, 316G, 316H, 316I, 316K, 316L, 316M, 316N, 316P, 316Q, 316R, 316S, 316T, 316V, 316W, 316Y, 322A, 322C, 322D, 322E, 322F, 322G, 322H, 322K, 322L, 322M, 322N, 322P, 322Q, 322R, 322S, 322T, 322V, 322W, 322Y, 337A, 337C, 337D, 337E, 337F, 337G, 337H, 337I, 337K, 337L, 337M, 337N, 337Q, 337R, 337S, 337T, 337V, 337W, 337Y, 368C, 368D, 368E, 368F, 368G, 368H, 368I, 368K, 368L, 368M, 368N, 368P, 368Q, 368R, 368S, 368T, 368V, 368W, 368Y, 392A, 392C, 392D, 392E, 392F, 392G, 392H, 392I, 392K, 392L, 392M, 392N, 392P, 392Q, 392R, 392S, 392V, 392W 및 392Y로부터 선택된 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열번호 374를 기준으로 넘버링된다. 일부 실시양태에서, 재조합 알파-갈락토시다제 A는 서열번호 374에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 이상의 서열 동일성을 가지는 폴리펩타이드 서열 또는 이의 기능적 단편을 포함하고, 여기서, 상기 재조합 알파-갈락토시다제 A는 P10A, P10C, P10D, P10E, P10F, P10G, P10H, P10I, P10K, P10L, P10M, P10N, P10Q, P10R, P10S, P10T, P10V, P10W, P10Y, E39A, E39C, E39D, E39F, E39G, E39H, E39I, E39K, E39L, E39M, E39N, E39P, E39Q, E39R, E39S, E39T, E39V, E39W, E39Y, R44A, R44C, R44D, R44E, R44F, R44G, R44H, R44I, R44K, R44L, R44N, R44P, R44Q, R44S, R44T, R44V, R44W, R44Y, T47A, T47C, T47D, T47E, T47F, T47G, T47H, T47I, T47K, T47L, T47M, T47N, T47P, T47Q, T47R, T47S, T47V, T47W, T47Y, H92A, H92C, H92D, H92E, H92F, H92G, H92I, H92K, H92L, H92M, H92N, H92P, H92Q, H92R, H92S, H92T, H92V, H92W, H92Y, P166A, P166C, P166D, P166E, P166F, P166G, P166H, P166I, P166K, P166L, P166M, P166N, P166Q, P166R, P166S, P166T, P166V, P166W, P166Y, A206C, A206D, A206E, A206F, A206G, A206H, A206I, A206K, A206L, A206M, A206N, A206P, A206Q, A206R, A206S, A206T, A206V, A206W, A206Y, R217A, R217C, R217D, R217E, R217F, R217G, R217H, R217I, R217K, R217L, R217M, R217N, R217P, R217Q, R217S, R217T, R217V, R217W, R217Y, D247A, D247C, D247E, D247F, D247G, D247H, D247I, D247K, D247L, D247M, D247N, D247P, D247Q, D247R, D247S, D247T, D247V, D247W, D247Y, G261A, G261C, G261D, G261E, G261F, G261H, G261I, G261K, G261L, G261M, G261N, G261P, G261Q, G261R, G261S, G261T, G261V, G261W, G261Y, A271C, A271D, A271E, A271F, A271G, A271H, A271I, A271K, A271L, A271M, A271N, A271P, A271Q, A271R, A271S, A271T, A271V, A271W, A271Y, K302A, K302C, K302D, K302E, K302F, K302G, K302H, K302I, K302L, K302M, K302N, K302P, K302Q, K302R, K302S, K302T, K302V, K302W, K302Y, D316A, D316C, D316E, D316F, D316G, D316H, D316I, D316K, D316L, D316M, D316N, D316P, D316Q, D316R, D316S, D316T, D316V, D316W, D316Y, I322A, I322C, I322D, I322E, I322F, I322G, I322H, I322K, I322L, I322M, I322N, I322P, I322Q, I322R, I322S, I322T, I322V, I322W, I322Y, P337A, P337C, P337D, P337E, P337F, P337G, P337H, P337I, P337K, P337L, P337M, P337N, P337Q, P337R, P337S, P337T, P337V, P337W, P337Y, A368C, A368D, A368E, A368F, A368G, A368H, A368I, A368K, A368L, A368M, A368N, A368P, A368Q, A368R, A368S, A368T, A368V, A368W, A368Y, T392A, T392C, T392D, T392E, T392F, T392G, T392H, T392I, T392K, T392L, T392M, T392N, T392P, T392Q, T392R, T392S, T392V, T392W 및 T392Y로부터 선택된 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열번호 374를 기준으로 넘버링된다.
일부 실시양태에서, 폴리뉴클레오타이드는 서열번호 1022에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 이상의 서열 동일성을 가지는 폴리펩타이드 서열 또는 이의 기능적 단편을 포함하는 재조합 알파-갈락토시다제 A를 코딩하고, 여기서, 상기 재조합 알파-갈락토시다제 A는 10, 10/392, 31, 31/39/44/166/302, 31/47, 31/283/284, 39, 39/44, 39/44/47, 39/44/47/261/283/284, 39/44/283, 39/44/339, 39/47/261, 39/92, 39/206, 39/284, 44, 44/284/302, 84, 84/92, 84/284/302/392, 84/316, 84/368/392, 92, 92/206/217, 92/206/275, 92/206/284, 92/206/302/368, 92/271, 92/271/277, 92/275/284, 92/283, 92/283/392, 92/284, 92/302, 92/316, 92/368, 155, 155/217, 155/368, 166, 166/283/284, 166/302, 206, 206/217, 206/334, 261, 261/283, 271, 271/368, 275, 283, 283/284, 283/392, 284, 302, 316, 334, 339, 368, 368/392 및 392로부터 선택된 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열번호 1022를 기준으로 넘버링된다. 일부 실시양태에서, 재조합 알파-갈락토시다제 A는 서열번호 1022에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 이상의 서열 동일성을 가지는 폴리펩타이드 서열 또는 이의 기능적 단편을 포함하고, 여기서, 상기 재조합 알파-갈락토시다제 A는 P10G, P10G/T392D, S31T, S31T/E39V/R44V/P166D/K302Y, S31T/T47R, S31T/K283L/D284A, E39L, E39L/H92V, E39L/A206E, E39L/D284S, E39V/R44V, E39V/R44V/T47R, E39V/R44V/T47R/G261S/K283L/D284A, E39V/R44V/K283T, E39V/R44V/A339N, E39V/T47R/G261S, R44V, R44V/D284E/K302Y, H84K, H84K/H92V, H84K/D284S/K302L/T392A, H84K/D316H, H84K/A368E/T392A, H92Q, H92T, H92T/A206E/R217N, H92T/A206E/K302T/A368E, H92T/A271K, H92T/A271K/K277R, H92T/K283P, H92T/K283V/T392W, H92T/D284M, H92T/K302L, H92T/A368E, H92V, H92V/A206E/D284S, H92V/A206Y/Q275A, H92V/Q275A/D284S, H92V/D284S, H92V/K302L, H92V/D316H, H155F, H155F/R217I, H155F/A368E, P166D, P166D/K283L/D284A, P166D/K302Y, A206E, A206E/R217N, A206I, A206Q, A206T/Y334C, A206Y, G261S, G261S/K283L, A271K, A271K/A368E, Q275A, K283L, K283P/T392W, K283T, K283T/D284E, D284E, D284M, D284S, K302L, K302Y, D316H, Y334C, A339N, A368E, A368E/T392W, T392A, T392D 및 T392W로부터 선택된 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열번호 1022를 기준으로 넘버링된다. 일부 실시양태에서, 재조합 알파-갈락토시다제 A는 서열번호 1022에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 이상의 서열 동일성을 가지는 폴리펩타이드 서열 또는 이의 기능적 단편을 포함하고, 여기서, 상기 재조합 알파-갈락토시다제 A는 10G, 10G/392D, 31T, 31T/39V/44V/166D/302Y, 31T/47R, 31T/283L/284A, 39L, 39L/92V, 39L/206E, 39L/284S, 39V/44V, 39V/44V/47R, 39V/44V/47R/261S/283L/284A, 39V/44V/283T, 39V/44V/339N, 39V/47R/261S, 44V, 44V/284E/302Y, 84K, 84K/92V, 84K/284S/302L/392A, 84K/316H, 84K/368E/392A, 92Q, 92T, 92T/206E/217N, 92T/206E/302T/368E, 92T/271K, 92T/271K/277R, 92T/283P, 92T/283V/392W, 92T/284M, 92T/302L, 92T/368E, 92V, 92V/206E/284S, 92V/206Y/275A, 92V/275A/284S, 92V/284S, 92V/302L, 92V/316H, 155F, 155F/217I, 155F/368E, 166D, 166D/283L/284A, 166D/302Y, 206E, 206E/217N, 206I, 206Q, 206T/334C, 206Y, 261S, 261S/283L, 271K, 271K/368E, 275A, 283L, 283P/392W, 283T, 283T/284E, 284E, 284M, 284S, 302L, 302Y, 316H, 334C, 339N, 368E, 368E/392W, 392A, 392D 및 392W로부터 선택된 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩타이드 서열의 아미노산 위치는 서열번호 1022를 기준으로 넘버링된다.
일부 실시양태에서, 전술된 바와 같이, 폴리뉴클레오타이드는 본원에 개시된 성질과 함께 GLA 활성을 가진 조작된 폴리펩타이드를 코딩하고, 여기서, 상기 폴리펩타이드는 기준 서열(예를 들어, 서열번호 2, 8, 58, 158, 372, 374, 704 및 1022) 또는 본원의 임의의 표에 개시된 임의의 변이체의 아미노산 서열에 대해 적어도 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 이상의 동일성, 및 서열번호 8의 기준 폴리펩타이드 또는 표 2-1, 5-1, 6-1, 7-1, 8-1, 9-1, 11-1, 12-1 및/또는 13-1에 개시된 임의의 변이체의 아미노산 서열에 비해 하나 이상의 잔기 차이(예를 들어, 1개, 2개, 3개, 4개, 5개, 6개, 7개, 8개, 9개, 10개 이상의 아미노산 잔기 위치)를 가진 아미노산 서열을 포함한다. 일부 실시양태에서, 폴리뉴클레오타이드는 본원에 개시된 성질과 함께 GLA 활성을 가진 조작된 폴리펩타이드를 코딩하고, 여기서, 상기 폴리펩타이드는 서열번호 2, 8, 58, 158, 372, 374, 704 및/또는 1022에 대해 최적으로 정렬될 때 기준 서열 서열번호 2, 8, 58, 158, 372, 374, 704 및/또는 1022에 대해 적어도 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 이상의 서열 동일성, 및 표 2-1, 5-1, 6-1, 7-1, 8-1, 9-1, 11-1, 12-1 및/또는 13-1에서 제공된 잔기 위치들로부터 선택된 잔기 위치에서 서열번호 2, 8, 58, 158, 372, 374, 704 및/또는 1022에 비해 하나 이상의 잔기 차이를 가진 아미노산 서열을 포함한다.
일부 실시양태에서, 조작된 GLA 폴리펩타이드를 코딩하는 폴리뉴클레오타이드는 서열번호 8, 58, 158, 372, 374, 704 및/또는 1022의 폴리뉴클레오타이드 서열을 포함한다. 일부 실시양태에서, 상기 폴리뉴클레오타이드는 고엄격도 조건 하에서 기준 폴리뉴클레오타이드 서열에 하이브리드화할 수 있다. 일부 실시양태에서, 기준 서열은 서열번호 1, 7, 57, 157, 275, 371, 373 및/또는 1019로부터 선택되거나, 이의 상보체이거나, 본원에 제공된 변이체 GLA 폴리펩타이드들 중 임의의 변이체 GLA 폴리펩타이드를 코딩하는 폴리뉴클레오타이드 서열이다. 일부 실시양태에서, 고엄격도 조건 하에서 하이브리드화할 수 있는 폴리뉴클레오타이드는 표 2-1, 5-1, 6-1, 7-1, 8-1, 9-1, 11-1, 12-1 및/또는 13-1에 기재된 임의의 위치들로부터 선택된 잔기 위치에서 서열번호 2, 8, 58, 158, 372, 374, 704 및/또는 1022에 비해 하나 이상의 잔기 차이를 가진 아미노산 서열을 포함하는 GLA 폴리펩타이드를 코딩한다.
일부 실시양태에서, 본원에 제공된 조작된 GLA 폴리펩타이드들 중 임의의 조작된 GLA 폴리펩타이드를 코딩하는 단리된 폴리뉴클레오타이드는 상기 폴리펩타이드의 발현을 제공하도록 다양한 방식들에 의해 조작된다. 일부 실시양태에서, 상기 폴리펩타이드를 코딩하는 폴리뉴클레오타이드는 상기 폴리뉴클레오타이드 및/또는 폴리펩타이드의 발현을 조절하는 하나 이상의 조절 서열이 존재하는 발현 벡터로서 제공된다. 단리된 폴리뉴클레오타이드를 벡터 내로 삽입하기 전에 조작하는 것은 발현 벡터에 따라 바람직할 수 있거나 필요할 수 있다. 재조합 DNA 방법을 이용하여 폴리뉴클레오타이드 및 핵산 서열을 변형시키는 기법은 당분야에서 잘 공지되어 있다.
일부 실시양태에서, 조절 서열은 다른 서열들 중에서 프로모터, 코작(Kozak) 서열, 리더 서열, 폴리아데닐화 서열, 전구펩타이드 서열, 신호 펩타이드 서열, 유전자 요법 보유를 위한 DNA 기반 조절 요소 및 전사 종결자를 포함한다. 당분야에서 공지되어 있는 바와 같이, 사용되는 숙주 세포를 기반으로 적합한 프로모터를 선택할 수 있다. 사상 진균 숙주 세포를 위한 예시적인 프로모터는 아스퍼길루스 오리재(Aspergillus oryzae) TAKA 아밀라제(amylase), 리조뮤코르 미에헤이(Rhizomucor miehei) 아스파르트 단백질분해효소, 아스퍼길루스 니게르(Aspergillus niger) 중성 알파 아밀라제, 아스퍼길루스 니게르 산 안정성 알파 아밀라제, 아스퍼길루스 니게르 또는 아스퍼길루스 아와모리(Aspergillus awamori) 글루코아밀라제(glucoamylase)(glaA), 리조뮤코르 미에헤이 라파제(lipase), 아스퍼길루스 오리재 알칼리성 단백질분해효소, 아스퍼길루스 오리재 트리오스 포스페이트 이소머라제(isomerase), 아스퍼길루스 니둘란스(Aspergillus nidulans) 아세트아미다제(acetamidase) 및 푸사리움 옥시스포룸(Fusarium oxysporum) 트립신 유사 단백질분해효소(예를 들어, WO 제96/00787호 참조)에 대한 유전자로부터 수득된 프로모터뿐만 아니라, NA2-tpi 프로모터(아스퍼길루스 니게르 중성 알파 아밀라제 및 아스퍼길루스 오리재 트리오스 포스페이트 이소머라제에 대한 유전자로부터의 프로모터들의 하이브리드), 및 이들의 돌연변이체 프로모터, 절단된 프로모터 및 하이브리드 프로모터도 포함한다. 예시적인 효모 세포 프로모터는 사카로마이세스 세레비지애(Saccharomyces cerevisiae) 에놀라제(enolase)(ENO-1), 사카로마이세스 세레비지애 갈락토키나제(galactokinase)(GAL1), 사카로마이세스 세레비지애 알코올 데하이드로게나제(dehydrogenase)/글리세르알데하이드-3-포스페이트 데하이드로게나제(ADH2/GAP), 및 사카로마이세스 세레비지애 3-포스포글리세레이트 키나제에 대한 유전자로부터 유래할 수 있다. 효모 숙주 세포를 위한 다른 유용한 프로모터는 당분야에서 공지되어 있다(예를 들어, 문헌[Romanos et al., Yeast 8:423-488 [1992]] 참조). 포유류 세포에서 사용하기 위한 예시적인 프로모터는 사이토메갈로바이러스(CMV), CMV 인핸서와 융합된 닭 β-액틴 프로모터, 원숭이 액포형성 바이러스 40(SV40), 호모 사피엔스(Homo sapiens) 포스포글리세레이트 키나제, 베타 액틴, 연장 인자-1a 또는 글리세르알데하이드-3-포스페이트 데하이드로게나제, 또는 갈루스 갈루스(Gallus gallus) β-액틴의 프로모터들을 포함하나, 이들로 제한되지 않는다.
일부 실시양태에서, 조절 서열은 전사를 종결시키기 위해 숙주 세포에 의해 인식되는 서열인 적합한 전사 종결자 서열이다. 종결자 서열은 폴리펩타이드를 코딩하는 핵산 서열의 3' 말단에 작동 가능하게 연결된다. 선택된 숙주 세포에서 작용하는 임의의 종결자가 본 발명에서 사용된다. 예를 들어, 사상 진균 숙주 세포를 위한 예시적인 전사 종결자는 아스퍼길루스 오리재 TAKA 아밀라제, 아스퍼길루스 니게르 글루코아밀라제, 아스퍼길루스 니둘란스 안쓰라닐레이트 합성효소, 아스퍼길루스 니게르 알파 글루코시다제 및 푸사리움 옥시스포룸 트립신 유사 단백질분해효소에 대한 유전자로부터 수득될 수 있다. 효모 숙주 세포를 위한 예시적인 종결자는 사카로마이세스 세레비지애 에놀라제, 사카로마이세스 세레비지애 사이토크롬 C(CYC1) 및 사카로마이세스 세레비지애 글리세르알데하이드-3-포스페이트 데하이드로게나제에 대한 유전자로부터 수득될 수 있다. 효모 숙주 세포를 위한 다른 유용한 종결자는 당분야에서 공지되어 있다(예를 들어, 상기 문헌[Romanos et al.] 참조). 포유류 세포를 위한 예시적인 종결자는 사이토메갈로바이러스(CMV), 원숭이 액포형성 바이러스 40(SV40), 호모 사피엔스 성장 호르몬 hGH, 소 성장 호르몬 BGH 및 인간 또는 토끼 베타 글로불린의 종결자들을 포함하나, 이들로 제한되지 않는다.
일부 실시양태에서, 조절 서열은 적합한 리더 서열, 5'-캡 변형, 5' UTR 등이다. 일부 실시양태에서, 이 조절 서열 요소들은 mRNA 수송 및 번역에 관여하는 분자에의 결합을 매개하고, 5'-핵산외부용해성 분해를 억제하고, 캡 제거에 대한 내성을 부여한다. 리더 서열은 폴리펩타이드를 코딩하는 핵산 서열의 5' 말단에 작동 가능하게 연결된다. 선택된 숙주 세포에서 작용하는 임의의 리더 서열이 사용될 수 있다. 사상 진균 숙주 세포를 위한 예시적인 리더는 아스퍼길루스 오리재 TAKA 아밀라제 및 아스퍼길루스 니둘란스 트리오스 포스페이트 이소머라제에 대한 유전자로부터 수득된다. 효모 숙주 세포를 위한 적합한 리더는 사카로마이세스 세레비지애 에놀라제(ENO-1), 사카로마이세스 세레비지애 3-포스포글리세레이트 키나제, 사카로마이세스 세레비지애 알파 인자 및 사카로마이세스 세레비지애 알코올 데하이드로게나제/글리세르알데하이드-3-포스페이트 데하이드로게나제(ADH2/GAP)에 대한 유전자들로부터 수득된 리더들을 포함하나, 이들로 제한되지 않는다. 포유류 숙주 세포를 위한 적합한 리더는 오르토폭스바이러스 mRNA에 존재하는 5'-UTR 요소를 포함하나, 이들로 제한되지 않는다.
일부 실시양태에서, 조절 서열은 mRNA 수송 및 번역, 및 mRNA 반감기에 관여하는 단백질에의 결합을 매개하는, 단백질 코딩 핵산 서열의 3' 말단에 작동 가능하게 연결된 서열인 3' 비번역 핵산 영역 및 폴리아데닐화 꼬리 핵산 서열을 포함한다. 선택된 숙주 세포에서 작용하는 임의의 폴리아데닐화 서열 및 3' UTR이 본 발명에서 사용될 수 있다. 사상 진균 숙주 세포를 위한 예시적인 폴리아데닐화 서열은 아스퍼길루스 오리재 TAKA 아밀라제, 아스퍼길루스 니게르 글루코아밀라제, 아스퍼길루스 니둘란스 안쓰라닐레이트 합성효소, 푸사리움 옥시스포룸 트립신 유사 단백질분해효소 및 아스퍼길루스 니게르 알파-글루코시다제에 대한 유전자들의 폴리아데닐화 서열들을 포함하나, 이들로 제한되지 않는다. 효모 숙주 세포를 위한 유용한 폴리아데닐화 서열도 당분야에서 공지되어 있다(예를 들어, 문헌[Guo and Sherman, Mol. Cell. Biol., 15:5983-5990 [1995]] 참조). 포유류 숙주 세포를 위한 유용한 폴리아데닐화 및 3' UTR 서열은 mRNA의 안정성 및 번역을 증가시키는 여러 서열 요소들을 가진 α-글로빈 mRNA 및 β-글로빈 mRNA의 3' UTR들을 포함하나, 이들로 제한되지 않는다.
일부 실시양태에서, 조절 서열은 폴리펩타이드의 아미노 말단에 연결된 아미노산 서열을 코딩하고 코딩된 폴리펩타이드가 세포의 분비 경로 내로 향하게 하는 신호 펩타이드 코딩 영역이다. 핵산 서열의 코딩 서열의 5' 말단은 분비된 폴리펩타이드를 코딩하는 코딩 영역의 분절과 번역 리딩 프레임으로 천연적으로 연결된 신호 펩타이드 코딩 영역을 본질적으로 함유할 수 있다. 대안적으로, 코딩 서열의 5' 말단은 코딩 서열과 이질적인 신호 펩타이드 코딩 영역을 함유할 수 있다. 발현된 폴리펩타이드가 선택된 숙주 세포의 분비 경로 내로 향하게 하는 임의의 신호 펩타이드 코딩 영역이 본원에 제공된 조작된 GLA 폴리펩타이드의 발현을 위해 사용된다. 사상 진균 숙주 세포를 위한 효과적인 신호 펩타이드 코딩 영역은 아스퍼길루스 오리재 TAKA 아밀라제, 아스퍼길루스니게르 중성 아밀라제, 아스퍼길루스 니게르 글루코아밀라제, 리조뮤코르 미에헤이 아스파르트 단백질분해효소, 후미콜라 인솔렌스(Humicola insolens) 셀룰라제(cellulase), 및 후미콜라 라누기노사(Humicola lanuginosa) 리파제에 대한 유전자들로부터 수득된 신호 펩타이드 코딩 영역들을 포함하나, 이들로 제한되지 않는다. 효모 숙주 세포를 위한 유용한 신호 펩타이드는 사카로마이세스 세레비지애 알파 인자 및 사카로마이세스 세레비지애 인버타제(invertase)에 대한 유전자들의 신호 펩타이드들을 포함하나, 이들로 제한되지 않는다. 포유류 숙주 세포를 위한 유용한 신호 펩타이드는 면역글로불린 감마(IgG)에 대한 유전자들의 신호 펩타이드들을 포함하나, 이들로 제한되지 않는다.
일부 실시양태에서, 조절 서열은 폴리펩타이드의 아미노 말단에 위치된 아미노산 서열을 코딩하는 전구펩타이드 코딩 영역이다. 생성된 폴리펩타이드는 일부 경우 "전구효소", "전구폴리펩타이드" 또는 "자이모겐"으로서 지칭된다. 전구폴리펩타이드는 전구폴리펩타이드로부터의 전구펩타이드의 촉매 또는 자가촉매 절단에 의해 성숙 활성 폴리펩타이드로 전환될 수 있다.
또 다른 양태에서, 본 발명은 재조합 발현 벡터가 도입되어야 하는 숙주의 유형에 따라 조작된 GLA 폴리펩타이드를 코딩하는 폴리뉴클레오타이드, 및 하나 이상의 발현 조절 영역, 예컨대, 프로모터 및 종결자, 복제 기점 등을 포함하는 재조합 발현 벡터도 제공한다. 일부 실시양태에서, 전술된 다양한 핵산 및 조절 서열들을 함께 연결하여 재조합 발현 벡터를 생성하고, 이 재조합 발현 벡터는 하나 이상의 편리한 제한 부위를 포함함으로써, 변이체 GLA 폴리펩타이드를 코딩하는 핵산 서열이 이러한 부위에서 삽입될 수 있게 하거나 치환될 수 있게 한다. 대안적으로, 본 발명의 폴리뉴클레오타이드 서열(들)은 폴리뉴클레오타이드 서열 또는 폴리뉴클레오타이드 서열을 포함하는 핵산 작제물을 발현에 적절한 벡터 내로 삽입함으로써 발현된다. 발현 벡터를 생성함에 있어서, 코딩 서열은 코딩 서열이 발현에 적절한 조절 서열과 작동 가능하게 연결되도록 상기 벡터에 위치된다.
재조합 발현 벡터는 재조합 DNA 절차에 의해 편리하게 처리될 수 있고 변이체 GLA 폴리뉴클레오타이드 서열의 발현을 야기할 수 있는 임의의 벡터(예를 들어, 아데노바이러스(AV), 아데노-관련 바이러스(AAV), 렌티바이러스(LV) 및 비-바이러스 벡터, 예컨대, 리포좀을 포함하나 이들로 제한되지 않는 플라스미드 또는 바이러스)일 수 있다. 벡터의 선택은 전형적으로 벡터와, 벡터가 도입되는 숙주 세포의 병용 가능성에 의해 좌우될 것이다. 벡터는 선형 또는 폐쇄된 환형 플라스미드일 수 있다.
일부 실시양태에서, 발현 벡터는 자가 복제 벡터(즉, 염색체외 물질로서 존재하는 벡터로서, 이의 복제가 염색체 복제와 무관한 벡터, 예컨대, 플라스미드, 염색체외 요소, 미니염색체 또는 인공 염색체)이다. 벡터는 자가 복제를 보장하기 위한 임의의 수단을 함유할 수 있다. 일부 대안적 실시양태에서, 벡터는 숙주 세포 내로 도입될 때 게놈 내로 통합되고 그 자신이 통합된 염색체(들)와 함께 복제되는 벡터일 수 있다. 나아가, 숙주 세포의 게놈 내로 도입될 총 DNA 또는 트랜스포존을 함께 함유하는 단일 벡터 또는 플라스미드, 또는 2개 이상의 벡터들 또는 플라스미드들이 사용될 수 있다.
일부 실시양태에서, 발현 벡터는 바람직하게는 형질전환된 세포를 용이하게 선택할 수 있게 하는 하나 이상의 선택 마커를 함유한다. "선택 마커"는 살생물제 또는 바이러스 내성, 중금속에 대한 내성, 영양요구주에 대한 원영양성(prototrophy) 등을 제공하는 생성물을 생성하는 유전자이다. 효모 숙주 세포를 위한 적합한 마커는 ADE2, HIS3, LEU2, LYS2, MET3, TRP1 및 URA3을 포함하나, 이들로 제한되지 않는다. 사상 진균 숙주 세포에 사용하기 위한 선택 마커는 amdS(아세트아미다제), argB(오르니틴 카르바모일트랜스퍼라제), bar(포스피노쓰리신 아세틸트랜스퍼라제), hph(하이그로마이신 포스포트랜스퍼라제), niaD(니트레이트 환원효소), pyrG(오로티딘-5'-포스페이트 데카르복실라제), sC(설페이트 아데닐트랜스퍼라제) 및 trpC(안쓰라닐레이트 합성효소)뿐만 아니라 이들의 균등물도 포함하나, 이들로 제한되지 않는다. 또 다른 양태에서, 본 발명은 본 출원의 적어도 하나의 조작된 GLA 폴리펩타이드를 코딩하는 폴리뉴클레오타이드를 포함하는 숙주 세포를 제공하고, 여기서, 상기 폴리뉴클레오타이드는 숙주 세포에서 조작된 GLA 효소(들)를 발현하도록 하나 이상의 조절 서열에 작동 가능하게 연결된다. 본 발명의 발현 벡터에 의해 코딩된 폴리펩타이드를 발현하는 데 사용될 숙주 세포는 당분야에서 잘 공지되어 있고, 진균 세포, 예컨대, 효모 세포(예를 들어, 사카로마이세스 세레비지애 및 피키아 파스토리스[예를 들어, ATCC 접수번호 201178]); 곤충 세포(예를 들어, 드로소필라 S2 및 스포도프테라 Sf9 세포), 식물 세포, 동물 세포(예를 들어, CHO, CHO-K1, COS 및 BHK), 및 인간 세포(예를 들어, HEK293T, 인간 섬유모세포, THP-1, Jurkat 및 Bowes 흑색종 세포주)를 포함하나, 이들로 제한되지 않는다.
따라서, 또 다른 양태에서, 본 발명은 조작된 GLA 폴리펩타이드를 제조하는 방법으로서, 상기 폴리펩타이드의 발현에 적합한 조건 하에서 조작된 GLA 폴리펩타이드를 코딩하는 폴리뉴클레오타이드를 발현할 수 있는 숙주 세포를 배양하는 단계를 포함하는 방법을 제공한다. 일부 실시양태에서, 상기 방법은 본원에 기재된 바와 같이 GLA 폴리펩타이드를 단리하고/하거나 정제하는 단계를 추가로 포함한다.
전술된 숙주 세포를 위한 적절한 배양 배지 및 생장 조건은 당분야에서 잘 공지되어 있다. GLA 폴리펩타이드의 발현을 위한 폴리뉴클레오타이드는 당분야에서 공지되어 있는 다양한 방법들에 의해 세포 내로 도입될 수 있다. 기법들은 특히 전기천공, 바이올리스틱(biolistic) 입자 폭격, 리포좀 매개 형질감염, 염화칼슘 형질감염 및 원형질체 융합을 포함한다.
본원에 개시된 성질을 가진 조작된 GLA는 천연 생성 또는 조작된 GLA 폴리펩타이드를 코딩하는 폴리뉴클레오타이드를 당분야에서 공지되어 있는 돌연변이유발 및/또는 유도 진화 방법으로 본원에 기재된 바와 같이 처리함으로써 수득될 수 있다. 예시적인 유도 진화 기법은 돌연변이유발 및/또는 DNA 셔플링이다(예를 들어, 문헌[Stemmer, Proc. Natl. Acad. Sci. USA 91:10747-10751 [1994]]; WO 제95/22625호, WO 제97/0078호, WO 제97/35966호, WO 제98/27230호, WO 제00/42651호 및 WO 제01/75767호; 및 미국 특허 제6,537,746호 참조). 이용될 수 있는 다른 유도 진화 절차는 특히 스태거링(staggered) 연장 과정(StEP), 시험관내 재조합(예를 들어, 문헌[Zhao et al., Nat. Biotechnol., 16:258-261 [1998]] 참조), 돌연변이유발성 PCR(예를 들어, 문헌[Caldwell et al., PCR Methods Appl., 3:S136-S140 [1994]] 참조), 및 카세트 돌연변이유발(예를 들어, 문헌[Black et al., Proc. Natl. Acad. Sci. USA 93:3525-3529 [1996]] 참조)을 포함한다.
예를 들어, 돌연변이유발 및 유도 진화 방법을 폴리뉴클레오타이드에 용이하게 적용하여, 발현될 수 있고 스크리닝될 수 있고 어세이될 수 있는 변이체 라이브러리를 생성할 수 있다. 돌연변이유발 및 유도 진화 방법은 당분야에서 잘 공지되어 있다(예를 들어, 미국 특허 제5,605,793호, 제5,811,238호, 제5,830,721호, 제5,834,252호, 제5,837,458호, 제5,928,905호, 제6,096,548호, 제6,117,679호, 제6,132,970호, 제6,165,793호, 제6,180,406호, 제6,251,674호, 제6,277,638호, 제6,287,861호, 제6,287,862호, 제6,291,242호, 제6,297,053호, 제6,303,344호, 제6,309,883호, 제6,319,713호, 제6,319,714호, 제6,323,030호, 제6,326,204호, 제6,335,160호, 제6,335,198호, 제6,344,356호, 제6,352,859호, 제6,355,484호, 제6,358,740호, 제6,358,742호, 제6,365,377호, 제6,365,408호, 제6,368,861호, 제6,372,497호, 제6,376,246호, 제6,379,964호, 제6,387,702호, 제6,391,552호, 제6,391,640호, 제6,395,547호, 제6,406,855호, 제6,406,910호, 제6,413,745호, 제6,413,774호, 제6,420,175호, 제6,423,542호, 제6,426,224호, 제6,436,675호, 제6,444,468호, 제6,455,253호, 제6,479,652호, 제6,482,647호, 제6,489,146호, 제6,506,602호, 제6,506,603호, 제6,519,065호, 제6,521,453호, 제6,528,311호, 제6,537,746호, 제6,573,098호, 제6,576,467호, 제6,579,678호, 제6,586,182호, 제6,602,986호, 제6,613,514호, 제6,653,072호, 제6,716,631호, 제6,946,296호, 제6,961,664호, 제6,995,017호, 제7,024,312호, 제7,058,515호, 제7,105,297호, 제7,148,054호, 제7,288,375호, 제7,421,347호, 제7,430,477호, 제7,534,564호, 제7,620,500호, 제7,620,502호, 제7,629,170호, 제7,702,464호, 제7,747,391호, 제7,747,393호, 제7,751,986호, 제7,776,598호, 제7,783,428호, 제7,795,030호, 제7,853,410호, 제7,868,138호, 제7,873,499호, 제7,904,249호, 제7,957,912호, 제8,383,346호, 제8,504,498호, 제8,849,575호, 제8,876,066호, 제8,768,871호, 제9,593,326호, 및 모든 관련 비-미국 대응특허들; 문헌[Ling et al., Anal. Biochem., 254(2):157-78 [1997]; Dale et al., Meth. Mol. Biol., 57:369-74 [1996]; Smith, Ann. Rev. Genet., 19:423-462 [1985]; Botstein et al., Science, 229:1193-1201 [1985]; Carter, Biochem. J., 237:1-7 [1986]; Kramer et al., Cell, 38:879-887 [1984]; Wells et al., Gene, 34:315-323 [1985]; Minshull et al., Curr. Op. Chem. Biol., 3:284-290 [1999]; Christians et al., Nat. Biotechnol., 17:259-264 [1999]; Crameri et al., Nature, 391:288-291 [1998]; Crameri, et al., Nat. Biotechnol., 15:436-438 [1997]; Zhang et al., Proc. Nat. Acad. Sci. U.S.A., 94:4504-4509 [1997]; Crameri et al., Nat. Biotechnol., 14:315-319 [1996]; Stemmer, Nature, 370:389-391 [1994]; Stemmer, Proc. Nat. Acad. Sci. USA, 91:10747-10751 [1994]]; 미국 특허 출원 공개 제2008/0220990호, US 제2009/0312196호, US 제2014/0005057호, US 제2014/0214391호, US 제2014/0221216호; US 제2015/0050658호, US 제2015/0133307호, US 제2015/0134315호 및 모든 관련 비-미국 대응특허들; WO 제95/22625호, WO 제97/0078호, WO 제97/35966호, WO 제98/27230호, WO 제00/42651호, WO 제01/75767호 및 WO 제2009/152336호 참조; 이들 모두는 본원에 참조로 포함됨).
일부 실시양태에서, 돌연변이유발 처리 후 수득된 효소 변이체는 이 효소 변이체를 규정된 온도(또는 다른 어세이 조건)으로 처리하고 열 처리 또는 다른 어세이 조건 후 남아있는 효소 활성의 양을 측정함으로써 스크리닝된다. 그 다음, GLA 폴리펩타이드를 코딩하는 폴리뉴클레오타이드를 함유하는 DNA를 숙주 세포로부터 단리하고 시퀀싱하여 뉴클레오타이드 서열 변화(존재하는 경우)를 확인하고, 상이한 또는 동일한 숙주 세포에서 효소를 발현시키는 데 사용한다. 발현 라이브러리로부터 효소 활성을 측정하는 것은 당분야에서 공지되어 있는 임의의 적합한 방법을 이용함으로써 수행될 수 있다(예를 들어, 표준 생화학 기법, 예컨대, HPLC 분석).
공지된 서열의 조작된 폴리펩타이드의 경우, 효소를 코딩하는 폴리뉴클레오타이드는 공지된 합성 방법에 따라 표준 고체상 방법에 의해 제조될 수 있다. 일부 실시양태에서, 최대 약 100개 염기의 단편을 개별적으로 합성한 후, (예를 들어, 효소적 또는 화학적 라이게이션 방법, 또는 중합효소 매개 방법으로) 연결하여 임의의 원하는 연속 서열을 형성할 수 있다. 예를 들어, 본원에 개시된 폴리뉴클레오타이드 및 올리고뉴클레오타이드는 전형적으로 자동 합성 방법으로 실시되는 고전적인 포스포라미다이트 방법(예를 들어, 문헌[Beaucage et al., Tetra. Lett., 22:1859-69 [1981]]; 및 문헌[Matthes et al., EMBO J., 3:801-05 [1984]] 참조)을 이용한 화학적 합성에 의해 제조될 수 있다. 포스포라미다이트 방법에 따라, (예를 들어, 자동 DNA 합성기에서) 올리고뉴클레오타이드를 합성하고 정제하고 어닐링하고 라이게이션시키고 적절한 벡터에 클로닝한다.
따라서, 일부 실시양태에서, 조작된 GLA 폴리펩타이드를 제조하는 방법은 (a) 표 2-1, 5-1, 6-1, 7-1, 8-1, 9-1, 11-1, 12-1 및/또는 13-1에서 제공된 임의의 변이체의 아미노산 서열 및 서열번호 8, 58, 158, 372, 374, 704 및/또는 1022로부터 선택된 아미노산 서열을 포함하는 폴리펩타이드를 코딩하는 폴리뉴클레오타이드를 합성하는 단계, 및 (b) 상기 폴리뉴클레오타이드에 의해 코딩된 GLA 폴리펩타이드를 발현시키는 단계를 포함할 수 있다. 상기 방법의 일부 실시양태에서, 상기 폴리뉴클레오타이드에 의해 코딩된 아미노산 서열은 임의적으로 하나 또는 여러 개(예를 들어, 최대 3개, 4개, 5개 또는 최대 10개)의 아미노산 잔기 돌연변이(예를 들어, 결실, 삽입 및/또는 치환)을 가질 수 있다. 일부 실시양태에서, 아미노산 서열은 임의적으로 1개 또는 2개, 1개 내지 3개, 1개 내지 4개, 1개 내지 5개, 1개 내지 6개, 1개 내지 7개, 1개 내지 8개, 1개 내지 9개, 1개 내지 10개, 1개 내지 15개, 1개 내지 20개, 1개 내지 21개, 1개 내지 22개, 1개 내지 23개, 1개 내지 24개, 1개 내지 25개, 1개 내지 30개, 1개 내지 35개, 1개 내지 40개, 1개 내지 45개 또는 1개 내지 50개의 아미노산 잔기 돌연변이(예를 들어, 결실, 삽입 및/또는 치환)을 가진다. 일부 실시양태에서, 아미노산 서열은 임의적으로 1개, 2개, 3개, 4개, 5개, 6개, 7개, 8개, 9개, 10개, 11개, 12개, 13개, 14개, 15개, 16개, 17개, 18개, 19개, 20개, 21개, 22개, 23개, 24개, 25개, 30개, 35개, 40개, 45개 또는 50개의 아미노산 잔기 돌연변이(예를 들어, 결실, 삽입 및/또는 치환)을 가진다. 일부 실시양태에서, 아미노산 서열은 임의적으로 1개, 2개, 3개, 4개, 5개, 6개, 7개, 8개, 9개, 10개, 11개, 12개, 13개, 14개, 15개, 16개, 18개, 20개, 21개, 22개, 23개, 24개 또는 25개의 아미노산 잔기 돌연변이(예를 들어, 결실, 삽입 및/또는 치환)을 가진다. 일부 실시양태에서, 치환은 보존적 또는 비보존적 치환일 수 있다.
본원에 기재된 어세이 및 조건을 포함하나 이들로 제한되지 않는, 당분야에서 공지되어 있는 임의의 적합한 어세이를 이용하여, 발현된 조작된 GLA 폴리펩타이드를 임의의 원하는 개선된 성질(예를 들어, 활성, 선택성, 안정성, 산 내성, 단백질분해효소 민감성 등)에 대해 평가할 수 있다.
일부 실시양태에서, 특히 라이소자임 처리, 초음파처리, 여과, 염석, 한외원심분리 및 크로마토그래피를 포함하는, 잘 공지되어 있는 단백질 정제 기법들 중 어느 하나 이상의 정제 기법을 이용하여, 숙주 세포에서 발현된 조작된 GLA 폴리펩타이드들 중 임의의 조작된 GLA 폴리펩타이드를 세포 및/또는 배양 배지로부터 회수한다.
GLA 폴리펩타이드를 단리하는 크로마토그래피 기법은 특히, 역상 크로마토그래피 고성능 액체 크로마토그래피, 이온 교환 크로마토그래피, 소수성 상호작용 크로마토그래피, 겔 전기영동 및 친화성 크로마토그래피를 포함한다. 특정 효소를 정제하는 조건은 부분적으로 순전하, 소수성, 친수성, 분자량, 분자 모양 등과 같은 요인에 의해 좌우되고, 당분야에서 기술을 가진 자에게 자명할 것이다. 일부 실시양태에서, 친화성 기법을 이용하여 개선된 변이체 GLA 효소를 단리할 수 있다. 친화성 크로마토그래피 정제를 이용하는 일부 실시양태에서, 변이체 GLA 폴리펩타이드에 특이적으로 결합하는 임의의 항체가 사용된다. 친화성 크로마토그래피 정제를 이용하는 일부 실시양태에서, GLA에 공유부착된 글리칸에 결합하는 단백질이 사용된다. 친화성 크로마토그래피 정제를 이용하는 추가의 다른 실시양태에서, GLA 활성 부위에 결합하는 임의의 소분자가 사용된다. 항체의 제조를 위해, 토끼, 마우스, 래트 등을 포함하나 이들로 제한되지 않는 다양한 숙주 동물들은 GLA 폴리펩타이드(예를 들어, GLA 변이체) 또는 이의 단편을 주사함으로써 면역화된다. 일부 실시양태에서, GLA 폴리펩타이드 또는 단편은 측쇄 작용기, 또는 측쇄 작용기에 부착된 링커에 의해 BSA와 같은 적합한 담체에 부착된다.
일부 실시양태에서, 조작된 GLA 폴리펩타이드는 조작된 GLA 폴리펩타이드의 생성에 유리한 조건 하에서 본원에 기재된 바와 같은 조작된 GLA 폴리펩타이드를 코딩하는 폴리뉴클레오타이드 서열을 포함하는 숙주 세포(예를 들어, 사카로마이세스 세레비지애, 다우쿠스 카로타(Daucus carota), 니코티아나 타바쿰(Nicotiana tabacum), 호모 사피엔스(H. sapiens)(예를 들어, HEK293T) 또는 크리세툴루스 그리세우스(Cricetulus griseus)(예를 들어, CHO))를 배양하는 단계 및 세포 및/또는 배양 배지로부터 조작된 GLA 폴리펩타이드를 회수하는 단계를 포함하는 방법에 의해 숙주 세포에서 생성된다.
일부 실시양태에서, 본 발명은 조작된 GLA 폴리펩타이드의 생성을 허용하는 적합한 배양 조건 하에서, 서열번호8, 58, 158, 372, 374, 704, 및/또는 1022의 아미노산 서열과 최적으로 정렬될 때 기준 서열(예를 들어, 서열번호 8, 58, 158, 372, 374, 704, 및/또는 1022)에 대해 적어도 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 95%, 96%, 97%, 98%, 99% 또는 100%의 서열 동일성, 및 표 2-1, 5-1, 6-1, 7-1, 8-1, 9-1, 11-1, 12-1, 및 13-1에서 제공된 것들, 및/또는 이들의 조합으로부터 선택된, 서열번호 8, 58, 158, 372, 374, 704, 및/또는 1022에 비해 하나 이상의 아미노산 잔기 차이를 가진 조작된 GLA 폴리펩타이드를 코딩하는 폴리뉴클레오타이드 서열을 포함하는 재조합 진핵세포를 배양하는 단계, 및 임의적으로 배양물 및/또는 배양된 세포로부터 조작된 GLA 폴리펩타이드를 회수하는 단계를 포함하는 조작된 GLA 폴리펩타이드의 제조 방법을 포괄한다.
일부 실시양태에서, 일단 조작된 GLA 폴리펩타이드가 재조합 숙주 세포 또는 세포 배양 배지로부터 회수되면, 이 폴리펩타이드를 당분야에서 공지되어 있는 임의의 적합한 방법(들)으로 더 정제한다. 일부 추가 실시양태에서, 정제된 GLA 폴리펩타이드를 다른 성분 및 화합물과 조합하여 상이한 적용 및 용도에 적합한, 조작된 GLA 폴리펩타이드를 포함하는 조성물 및 제형(예를 들어, 약학 조성물)을 제공한다. 일부 추가 실시양태에서, 정제된 조작된 GLA 폴리펩타이드 또는 제형화된 조작된 GLA 폴리펩타이드를 동결건조한다. 일부 실시양태에서, 조작된 GLA 폴리펩타이드를 체내(즉, 인간 또는 또 다른 동물과 같은 체내 세포)에서 직접 생성하고 정제하지 않는다. 그러나, 일부 대안적 실시양태에서, 조작된 GLA 폴리펩타이드를 체내(즉, 인간 또는 또 다른 동물과 같은 체내 세포)에서 생성하고, 당분야에서 공지되어 있는 방법을 이용하여 신체로부터 회수한다. 일부 추가 실시양태에서, 이 회수된 조작된 GLA 폴리펩타이드를 정제한다. 또 다른 일부 추가 실시양태에서, 이 회수되고/되거나 정제된 조작된 폴리펩타이드를 또 다른 동물(예를 들어, 인간 또는 또 다른 동물) 내로 도입하거나, 회수되고/되거나 정제된 조작된 GLA 폴리펩타이드를 최초로 생성한 체내로 재도입한다.
조성물:
본 발명은 이하에 기재된 조성물 및 포맷을 포함하나 이들로 제한되지 않는 다양한 조성물들 및 포맷들을 제공한다. 일부 실시양태에서, 본 발명은 약학 조성물 및 다른 조성물, 예컨대, 식이/영양 보충제에 사용하기에 적합한 조작된 GLA 폴리펩타이드를 제공한다.
치료적 유효량의 본 발명에 따른 조작된 GLA를 포함하는 이 조성물은 투여 방식에 따라 고체, 반고체 또는 액체의 형태로 존재한다. 일부 실시양태에서, 상기 조성물은 다른 약학적으로 허용 가능한 성분, 예컨대, 희석제, 완충제, 부형제, 염, 유화제, 보존제, 안정화제, 충전제 및 다른 성분을 포함한다. 제형화 및 투여 기법에 대한 상세한 내용은 당분야에서 잘 공지되어 있고 문헌에 기재되어 있다. 일부 실시양태에서, 이 조성물은 유전자 요법으로서 도입된 후 인간 체내에서 직접 생성된다.
일부 실시양태에서, 조작된 GLA 폴리펩타이드는 약학 조성물에 사용되도록 제형화된다. 환제, 정제, 겔 탭, 캡슐, 로젠지, 당의정, 산제, 연질 겔, 졸-겔, 겔, 유화액, 이식물, 패치, 스프레이, 연고, 도포제, 크림, 페이스트, 젤리, 페인트, 에어로졸, 츄잉 검, 완화제, 스틱, 용액, 현탁액(오일 기제 현탁액, 수중유 유화액 등을 포함하나 이들로 제한되지 않음), 슬러리, 시럽, 조절 방출 제형, 좌약제 등을 포함하나 이들로 제한되지 않는, 조작된 GLA 폴리펩타이드를 전달하는 데 사용되는 임의의 적합한 포맷이 본 발명에서 사용된다. 일부 실시양태에서, 조작된 GLA 폴리펩타이드는 주사 또는 주입에 적합한 포맷(즉, 주사 제형)으로 제공된다. 일부 실시양태에서, 조작된 GLA 폴리펩타이드를 위한 폴리뉴클레오타이드 서열은 주사에 적합한 포맷으로 제공된다. 일부 실시양태에서, 조작된 GLA 폴리펩타이드는 실리카 기제(예를 들어, 옥시실란) 졸-겔을 포함하는 졸-겔과 같은 생체적합성 매트릭스로 제공된다. 일부 실시양태에서, 조작된 GLA 폴리펩타이드는 캡슐화된다. 일부 대안적 실시양태에서, 조작된 GLA 폴리펩타이드는 나노구조물(예를 들어, 나노튜브, 나노튜불, 나노캡슐 또는 마이크로캡슐, 마이크로스피어, 리포좀 등)로 캡슐화된다. 실제로, 본 발명은 임의의 특정 전달 제형 및/또는 전달 수단으로 제한되는 것으로 의도되지 않는다. 조작된 GLA 폴리펩타이드는 비경구, 경구, 국소, 경피, 비강내, 안구내, 척수강내, 이식물 등을 포함하나 이들로 제한되지 않는, 당분야에서 공지되어 있는 임의의 적합한 수단에 의해 투여되는 것으로 의도된다.
일부 실시양태에서, 조작된 GLA 폴리펩타이드는 글리코실화, 화학적 가교결합 시약, 페길화(즉, 폴리에틸렌 글리콜[PEG] 또는 활성화된 PEG 등으로 변형시킴) 또는 다른 화합물(예를 들어, 문헌[Ikeda, Amino Acids 29:283-287 [2005]]; 미국 특허 제7,531,341호, 제7,534,595호, 제7,560,263호 및 제7,53,653호; 미국 특허 출원 공개 제2013/0039898호 및 제2012/0177722호 등 참조)에 의해 화학적으로 변형된다. 실제로, 본 발명은 임의의 특정 전달 방법 및/또는 기작으로 제한되는 것으로 의도되지 않는다.
일부 추가 실시양태에서, 조작된 GLA 폴리펩타이드는 아데노바이러스(AV), 아데노-관련 바이러스(AAV), 렌티바이러스(LV)를 포함하나 이들로 제한되지 않는 바이러스 전달 벡터, 또는 비-바이러스 벡터(예를 들어, 리포좀)를 포함하는 유전자 요법을 통해 세포 또는 조직에게 전달되도록 제공된다. 일부 실시양태에서, 조작된 GLA 폴리펩타이드는 폴리리보뉴클레오타이드 서열을 캡슐화된 전달 비히클(예를 들어, 리포좀)으로 제형화한 후 mRNA 요법을 통해 세포 또는 조직에게 전달되도록 제공된다. 일부 추가 실시양태에서, 조작된 GLA 폴리펩타이드는 조작된 GLA 폴리펩타이드를 코딩하는 폴리뉴클레오타이드 서열을 외생성 세포 내로 도입하고 그 세포(또는 세포들)을 수용자(예를 들어, 파브리병을 나타내거나 발생시킬 위험을 가진 환자) 내로 도입하는 세포 요법을 통해 세포 또는 조직에게 전달되도록 제공된다.
일부 추가 실시양태에서, 조작된 GLA 폴리펩타이드는 매트릭스에 의해 안정화된 효소 결정을 포함하는 제형으로 제공된다. 일부 실시양태에서, 제형은 가교결합된 결정성 조작된 GLA 효소, 및 효소 결정에 부착하는 반응성 모이어티를 가진 중합체를 포함한다. 본 발명은 중합체 내의 조작된 GLA 폴리펩타이드도 제공한다.
일부 실시양태에서, 본 발명의 조작된 GLA 폴리펩타이드를 포함하는 조성물은 당(예를 들어, 락토스, 수크로스, 만니톨 및/또는 소르비톨), 전분(예를 들어, 옥수수, 밀, 쌀, 감자 또는 다른 식물 전분), 셀룰로스(예를 들어, 메틸 셀룰로스, 하이드록시프로필메틸 셀룰로스, 나트륨 카르복시-메틸셀룰로스), 검(예를 들어, 아라비아, 트라가칸쓰, 구아 등) 및/또는 단백질(예를 들어, 젤라틴, 콜라겐 등)을 포함하나 이들로 제한되지 않는 하나 이상의 통상적으로 사용되는 담체 화합물을 포함한다.
일부 실시양태에서, 본 발명은 혈액, 뇌척수액 등과 같은 유체에서 당지질의 농도를 감소시키는 데 사용하기에 적합한 조작된 GLA 폴리펩타이드를 제공한다. 투여되는 조작된 GLA 폴리펩타이드(들)의 용량은 병태 또는 질환, 대상체의 일반적인 상태, 및 당업자에게 공지되어 있는 다른 요인에 의해 좌우된다. 일부 실시양태에서, 조성물은 단회 또는 다회 투여를 위한 것이다. 일부 실시양태에서, 파브리병을 가진 인간에게 투여되는 조성물(들) 중의 조작된 GLA 폴리펩타이드(들)의 농도는 질환(예를 들어, 파브리병)을 효과적으로 치료하고/하거나 호전시키기에 충분할 것으로 예상된다. 일부 실시양태에서, 조작된 GLA 폴리펩타이드는 다른 약학 조성물 및/또는 식이 조성물과 함께 투여된다.
실험
실험 및 달성된 결과를 포함하는 하기 실시예는 예시 목적으로만 제공되고 본 발명을 제한하는 것으로서 해석되어서는 안된다. 하기 실험 개시에서, 하기 약어들이 적용된다: ppm(백만분율); M(몰); mM(밀리몰), uM 및 μM(마이크로몰); nM(나노몰); mol(몰); gm 및 g(그램); mg(밀리그램); ug 및 ㎍(마이크로그램); L 및 ℓ(리터); ㎖ 및 mL(밀리리터); cm(센티미터); mm(밀리미터); um 및 ㎛(마이크로미터); sec.(초); min(s)(분(s)); h(s) 및 hr(s)(시간(s)); U(유닛); MW(분자량); rpm(분당 회전); ℃(섭씨 온도); SEM(평균의 표준 오차); IV(정맥내); CDS(코딩 서열); DNA(데옥시리보핵산); RNA(리보핵산); 이. 콜라이(E. coli) W3110(통상적으로 사용되는 실험실 이. 콜라이 균주, 콜라이 제네틱 스톡 센터(Coli Genetic Stock Center)[CGSC]로부터 입수 가능함, 코넥티컷주 뉴 하벤 소재); NHP(비-인간 영장류); HPLC(고압 액체 크로마토그래피); MWCO(분자량 컷-오프); SDS-PAGE(도데실황산나트륨 폴리아크릴아미드 겔 전기영동); PBS(인산염 완충 식염수); DPBS(둘베코 인산염 완충 식염수); PES(폴리에테르설폰); CFSE(카르복시플루오레세인 석신이미딜 에스테르); IPTG(이소프로필 β-D-1-티오갈락토피라노사이드); PMBS(폴리믹신 B 설페이트); NADPH(니코틴아미드 아데닌 디뉴클레오타이드 포스페이트); GIDH(글루타메이트 데하이드로게나제); FIOPC(양성 대조군에 비해 배수 개선); PBMC(말초 혈액 단핵 세포); LB(루리아 브로쓰); MeOH(메탄올); Cmax(투여 간격 동안의 최대 약물 농도); RFU(상대 형광 단위); AUC0-t (곡선하 면적, 마지막 측정 가능한 농도까지); CL(제거); Vz(말기 단계 동안 겉보기 분포 부피); TI(시험 항목); 아텐스 리서치(Athens Research)(아텐스 리서치 테크놀로지(Athens Research Technology), 조지아주 아텐스 소재); 프로스펙(ProSpec)(프로스펙 타니테크노진(ProSpec Tany Technogene), 뉴저지주 이스트 브룬스윅 소재); 시그마-알드리치(Sigma-Aldrich)(시그마-알드리치, 미조리주 세인트 루이스 소재); 램 사이언티픽(Ram Scientific)(램 사이언티픽 인코포레이티드(Ram Scientific, Inc.), 뉴욕주 용커스 소재); 팔 코포레이션(Pall Corp.)(팔 코포레이션, 뉴욕주 포트 워싱톤 소재); 밀리포어(Millipore)(밀리포어 코포레이션(Millipore, Corp.), 매사추세츠주 빌러리카 소재); 딥코(Difco)(딥코 래보러토리스(Difco Laboratories), 비디 디아그노스틱 시스템스(BD Diagnostic Systems), 미시간주 디트로이트 소재); 퍼킨엘머(PerkinElmer)(퍼킨엘머, 매사추세츠주 월섬 소재); 몰레큘라 디바이시스(Molecular Devices)(몰레큘라 디바이시스 엘엘씨(Molecular Devices, LLC), 캘리포니아주 서니베일 소재); 퀴너(Kuhner)(아돌프 퀴너 아게(Adolf Kuhner, AG), 스위스 바젤 소재); 악시겐(Axygen)(악시겐 인코포레이티드(Axygen, Inc.), 캘리포니아주 유니온 시티 소재); 토론토 리서치 케미칼스(Toronto Research Chemicals)(토론토 리서치 케미칼스 인코포레이티드(Toronto Research Chemicals Inc.), 캐나다 온타리오 토론토 소재); 캠브리지 이소톱 래보러토리스(Cambridge Isotope Laboratories)(캠브리지 이소톱 래보러토리스 인코포레이티드(Cambridge Isotope Laboratories, Inc.), 매사추세츠주 턱스버리 소재); 어플라이드 바이오시스템스(Applied Biosystems)(어플라이드 바이오시스템스, 라이프 테크놀로지스 코포레이션(Life Technologies, Corp.)의 자회사, 뉴욕주 그랜드 아일랜드 소재), 아질런트(Agilent)(아질런트 테크놀로지스 인코포레이티드(Agilent Technologies, Inc.), 캘리포니아주 산타 클라라 소재); 써모 사이언티픽(Thermo Scientific)(써모피셔 사이언티픽(ThermoFisher Scientific)의 자회사, 매사추세츠주 월쌈 소재); 깁코(Gibco)(써모피셔 사이언티픽); 피어스(Pierce)(피어스 바이오테크놀로지(Pierce Biotechnology)(현재 써모피셔 사이언티픽의 자회사), 일리노이주 록포드 소재); 써모피셔 사이언티픽(써모피셔 사이언티픽, 매사추세츠주 월쌈 소재); 코닝(Corning)(코닝 인코포레이티드(Corning, Inc.), 캘리포니아주 팔로알토 소재); 제노텍(XenoTech)(세키스이 제노텍 엘엘씨(Sekisui XenoTech, LLC), 캔자스주 캔자스 시티 소재); 코리엘(Coriell) 의학 연구소(코리엘 의학 연구소, 뉴저지주 캠던 소재); VWR(VWR 인터네셔날(VWR International), 펜실베이니아주 래드너 소재); 잭슨(Jackson)(더 잭슨 래보러토리(The Jackson Laboratory), 메인주 바 하버 소재); 메가자임(Megazyme)(메가자임 인터내셔날(Megazyme International), 아일랜드 위클로우 소재); 엔조(Enzo)(엔조 라이프 사이언시스 인코포레이티드(Enzo Life Sciences, Inc.), 뉴욕주 파밍데일 소재); 지이 헬쓰케어(GE Healthcare)(지이 헬쓰케어 바이오-사이언시스(GE Healthcare Bio-Sciences), 뉴욕주 피스카타웨이 소재); LI-COR(LI-COR 바이오테크놀로지, 네브라스카주 링콘 소재); 아미쿠스(Amicus)(아미쿠스 테라퓨틱스(Amicus Therapeutics), 뉴저지주 크랜버리 소재); 페노메넥스(Phenomenex)(페노메넥스 인코포레이티드(Phenomenex, Inc.), 캘리포니아주 토란스 소재); 옵티멀(Optimal)(옵티멀 바이오텍 그룹(Optimal Biotech Group), 캘리포니아주 벨몬트 소재); 및 바이오-라드(Bio-Rad)(바이오-라드 래보러토리스(Bio-Rad Laboratories), 캘리포니아주 허큘리스 소재).
하기 폴리뉴클레오타이드 및 폴리펩타이드 서열들이 본 발명에서 사용된다. 일부 경우(하기 표시된 바와 같이), 폴리뉴클레오타이드 서열 다음에 코딩된 폴리펩타이드가 기재되어 있다.
전장 인간 GLA cDNA의 폴리뉴클레오타이드 서열 (SEQ ID NO.1):
전장 인간 GLA의 폴리펩타이드 서열:
성숙 효모 코돈 최적화된 (yCDS) 인간 GLA의 폴리뉴클레오타이드 서열:
성숙 인간 GLA의 폴리뉴클레오타이드 서열 (네이티브 hCDS):
성숙 인간 GLA의 폴리펩타이드 서열:
pCK110900i 이. 콜라이 발현 벡터의 폴리뉴클레오타이드 서열:
pYT-72Bgl 분비된 효모 발현 벡터의 폴리뉴클레오타이드 서열:
변이체 No. 73 yCDS의 폴리뉴클레오타이드 서열:
변이체 No. 73의 폴리뉴클레오타이드 서열:
변이체 No. 73의 폴리펩타이드 서열:
변이체 No. 218 yCDS의 폴리뉴클레오타이드 서열:
변이체 No. 218 hCDS의 폴리뉴클레오타이드 서열:
변이체 No. 218의 폴리펩타이드 서열:
변이체 No. 326 yCDS의 폴리뉴클레오타이드 서열:
변이체 No. 326의 폴리펩타이드 서열:
변이체 No. 206 yCDS의 폴리뉴클레오타이드 서열:
변이체 No. 206 hCDS의 폴리뉴클레오타이드 서열:
변이체 No. 206의 폴리펩타이드 서열:
변이체 No. 205 yCDS의 폴리뉴클레오타이드 서열:
변이체 No. 205 hCDS의 폴리뉴클레오타이드 서열:
변이체 No. 205의 폴리펩타이드 서열:
변이체 No. 76 yCDS의 폴리뉴클레오타이드 서열:
변이체 No. 76 hCDS의 폴리뉴클레오타이드 서열:
변이체 No. 76의 폴리펩타이드 서열:
Mfalpha 신호 펩타이드의 폴리뉴클레오타이드 서열:
Mfalpha 신호 펩타이드의 폴리펩타이드 서열:
MMO435의 폴리뉴클레오타이드 서열:
MMO439의 폴리뉴클레오타이드 서열:
MMO514의 폴리뉴클레오타이드 서열:
MMO481의 폴리뉴클레오타이드 서열:
합성 포유류 신호 펩타이드의 폴리뉴클레오타이드 서열:
LAKE Fw의 폴리뉴클레오타이드 서열:
Br 역방향의 폴리뉴클레오타이드 서열:
Br 정방향의 폴리뉴클레오타이드 서열:
hGLA 역방향의 폴리뉴클레오타이드 서열:
SP-GLA (yCDS)의 폴리뉴클레오타이드 서열:
MFleader-GLA (yCDS)의 폴리뉴클레오타이드 서열:
MFleader의 폴리펩타이드 서열:
변이체 No. 395 yCDS의 폴리뉴클레오타이드 서열:
변이체 No. 395의 폴리펩타이드 서열:
변이체 No. 402 yCDS의 폴리뉴클레오타이드 서열:
변이체 No. 402의 폴리펩타이드 서열:
변이체 No. 625 yCDS의 폴리뉴클레오타이드 서열:
변이체 No. 625의 폴리펩타이드 서열:
변이체 No. 648 yCDS의 폴리뉴클레오타이드 서열:
변이체 No. 648의 폴리펩타이드 서열:
실시예 1
GLA 유전자 획득 및 발현 벡터의 작제
WT 인간 GLA 서열(서열번호 2) 및 유도된 변이체(서열번호 4, 서열번호 6)를 코딩하는 합성 유전자(서열번호 1)를 이전에 기재된 바와 같이 작제하였다(예를 들어, 미국 특허 출원 공개 제2017/0360900 A1호 참조). 포유류 세포에서의 분비된 발현 및 일시적인 형질감염을 위해, 상이한 GLA 변이체들을 코딩하는 합성 유전자에 융합된 합성 마우스 IG 신호 펩타이드를 코딩하는 폴리뉴클레오타이드를 포함하는 키메라 GLA 발현 작제물을 다음과 같이 생성하였다. 올리고뉴클레오타이드 BamHI-pcDNA-GLA-F(서열번호 63) 및 XhoI-pcDNA-GLA-R(서열번호 64)을 사용하여, 신호 펩타이드를 코딩하는 단편 및 성숙 형태의 GLA 변이체에 대한 코딩 서열을 증폭시켰다. PCR 생성물을, BamHI/XhoI에 의해 선형화된 포유류 발현 벡터 pcDNA3.1(+)(인비트로겐(Invitrogen)), 또는 CMV 프로모터 및 BGH-pA(소 성장 호르몬 폴리아데닐화) 서열을 함유하는 벡터에 라이게이션시켰다. 당분야에서 숙련된 자에 의해 일반적으로 공지되어 있는 유도 진화 기법을 이용하여, 이 플라스미드 작제물 내의 서열번호 8로부터 유도된 유전자 변이체를 생성하였다(예를 들어, 미국 특허 제8,383,346호 및 WO 제2010/144103호 참조).
실시예 2
고처리율 생장 및 어세이
GLA 및 GLA 변이체의 고처리율(HTP) 생장
LIPOFECTAMINE® 3000 시약(써모피셔 사이언티픽)을 사용한 리포펙션 방법을 이용하여 야생형 GLA 또는 GLA 변이체에 융합된 합성 마우스 IG 신호 펩타이드를 코딩하는 pcDNA 3.1(+) 또는 CMV 프로모터 및 BGH-pA 함유 벡터로 HEK 293T 세포를 형질감염시켰다. HEK 293T 세포를 생장 배지(10% 소 태아 혈청을 가진 DMEM[둘 다 코닝(Corning)으로부터 입수])에서 배양하였다. 형질감염 24시간 전, 세포를 생장 배지 중의 105개 세포/웰/250 ㎕의 밀도로 NUNC® Edge 2.0 96웰 플레이트(써모피셔 사이언티픽)에 시딩하고 인큐베이터 내에서 37℃ 및 5% CO2에서 인큐베이션하였다. GLA 변이체가 발현되고 분비되도록 세포를 37℃ 및 5% CO2에서 24시간 내지 72시간 동안 인큐베이션하였다. 활성 및/또는 안정성 분석을 위해 HEK293T 형질감염으로부터의 컨디셔닝된 배지(50 내지 100 ㎕)를 코닝 96웰 고체 흑색 플레이트(코닝) 내로 옮겼다.
상청액의 HTP 분석
4-메틸움벨리페릴 α-D-갈락토피라노사이드(MUGal)의 가수분해를 측정함으로써 GLA 변이체 활성을 측정하였다. 비챌린지 어세이를 위해, 전술된 바와 같이 생성된 50 ㎕의 HEK 293T 컨디셔닝된 배지를 96웰 흑색 불투명 바닥 마이크로타이터 플레이트에서 50 ㎕의 McIlvaine 완충제(McIlvaine, J. Biol. Chem., 49:183-186 [1921])(pH 4.8) 중의 1 mM MUGal과 혼합하였다. 반응물을 짧게 혼합하고 30분 내지 180분 동안 37℃에서 인큐베이션한 후, 100 ㎕의 0.5 M 탄산나트륨(pH 10.2)으로 켄칭하였다. 형광(여기 355 nm, 방출 460 nm)을 모니터링하는 SPECTRAMAX® M2 마이크로플레이트 판독기를 이용하여 가수분해를 분석하였다. 이 어세이의 결과는 표 2-1에 제시되어 있다.
산으로 전처리된 상청액의 HTP 분석
GLA 변이체를 산성 완충제로 챌린지하여, 상기 변이체가 라이소좀 내부에서 접할 수 있는 극한 pH를 시뮬레이션하였다. 먼저, 50 ㎕의 HEK 293T 컨디셔닝된 배지 및 50 ㎕의 McIlvaine 완충제(pH 3.3 내지 4.3)를 96웰 환저 마이크로타이터 플레이트의 웰에 첨가하였다. 상기 플레이트를 PlateLoc® 열 마이크로플레이트 밀봉기(아질런트)로 밀봉하고 1시간 내지 2시간 동안 37℃에서 인큐베이션하였다. pH 4 챌린지 어세이를 위해, 50 ㎕의 산-pH-챌린지 샘플을 50 ㎕의 McIlvaine 완충제(pH 4.4) 중의 1 mM MUGal과 혼합하였다. 반응물을 짧게 혼합하고 30분 내지 180분 동안 37℃에서 인큐베이션한 후, 100 ㎕의 0.5 M 탄산나트륨(pH 10.2)으로 켄칭하였다. 형광(여기 355 nm, 방출 460 nm)을 모니터링하는 SPECTRAMAX® M2 마이크로플레이트 판독기를 이용하여 가수분해를 분석하였다. 이 어세이의 결과는 표 2-1에 제시되어 있다.
염기로 전처리된 상청액의 HTP 분석
GLA 변이체를 염기성(중성) 완충제로 챌린지하여, 상기 변이체가 환자에게 투여된 후 혈액에서 접하는 pH를 시뮬레이션하였다. 먼저, 50 ㎕의 GLA 변이체 HEK 293T 컨디셔닝된 배지 및 50 ㎕의 McIlvaine 완충제(pH 7.0 내지 8.2)를 96웰 환저 마이크로타이터 플레이트의 웰에 첨가하였다. 상기 플레이트를 밀봉하고 1시간 내지 18시간 동안 37℃에서 인큐베이션하였다. pH 7 챌린지 어세이를 위해, 50 ㎕의 염기성-pH-챌린지 샘플을 50 ㎕의 McIlvaine 완충제(pH 4.4) 중의 1 mM MUGal과 혼합하였다. 반응물을 짧게 혼합하고 30분 내지 180분 동안 37℃에서 인큐베이션한 후, 100 ㎕의 0.5 M 탄산나트륨(pH 10.2)으로 켄칭하였다. 형광(여기 355 nm, 방출 460 nm)을 모니터링하는 SPECTRAMAX® M2 마이크로플레이트 판독기를 이용하여 가수분해를 분석하였다. 이 어세이의 결과는 표 2-1에 제시되어 있다.
실시예 3
GLA 변이체의 생성
HEK293T 세포에서의 GLA의 생성
포유류 세포에서의 GLA 변이체의 분비된 발현을, HEK293, HEK293T, 또는 Expi293 세포의 일시적인 형질감염으로 수행하였다. 실시예 1에 기재된 바와 같이, 세포를 N-말단 합성 포유류 신호 펩타이드에 융합된 GLA 변이체(서열번호 3, 4, 9, 12, 17, 20, 23, 및 41)로 형질감염시키고 포유류 발현 벡터 pLEV113 내로 서브클로닝하였다. HEK293 세포를 플라스미드 DNA로 형질감염시켰고, 당분야에서 숙련된 자에게 공지되어 있는 표준 기법을 이용하여 4일 동안 현탁 생장시켰다. 상청액을 모으고 분석할 때까지 4℃에서 저장하였다.
실시예 4
GLA 변이체의 정제
포유류 세포 상청액으로부터 GLA 변이체의 정제
WT GLA(서열번호 2)를 문헌[Yasuda et al., Prot. Exp. Pur,. 37:499-506 [2004]]에 기재된 바와 같이 포유류 배양 상청액으로부터 정제하였다. 모든 다른 GLA 변이체들을 다음과 같이 정제하였다. 본질적으로 당분야에서 공지되어 있는 바와 같이 포유류 배양 상청액으로부터 GLA 변이체를 정제하였다(문헌[Yasuda et al., Prot. Exp. Pur,. 37:499-506 [2004]] 참조). 콘카나발린(Concanavalin) A 수지(시그마 알드리치)를 0.1 M 아세트산나트륨, 0.1 M NaCl, 1 mM MgCl2, CaCl2 및 MnCl2(pH 6.0)(콘카나발린 A 결합 완충제)로 평형화시켰다. 상청액을 0.2 ㎛ 병-탑(bottle-top) 필터로 멸균 여과한 후, 컬럼에 로딩하였다. 로딩 후, 컬럼을 10 컬럼 부피의 콘카나발린 A 결합 완충제로 세척하였고, 결합된 단백질을 0.9 M 메틸-α-D-만노피라노사이드 및 0.9 M 메틸-α-D-글루코피라노사이드로 보충된 콘카나발린 A 결합 완충제로 용출하였다. 용출된 단백질을 농축하였고 30 kDa 분자량 컷오프(밀리포어) 막을 가진 Amicon® Ultra 15 ㎖ 여과 유닛을 이용하여 완충제를 저장 완충제(20 mM 인산나트륨, 150 mM 염화나트륨, 185 μM TWEEN®-20 비이온성 세제, pH 6.0)로 교환하였다. 저장 완충제 중의 GLA를 ANOTOP® 0.2 ㎛ 주사기 필터(와트만(Whatman))를 통해 멸균 여과하고 -80℃에서 저장하였다. 정제는 BCA 정량에 기반할 때 배양 상청액 ㎖당 2.4 내지 50 ㎍의 정제된 단백질을 제공하였다.
BCA 단백질 어세이에 의한 단백질 정량
비신코닌산(BCA) 단백질 어세이(시그마 알드리치)를 이용하여 정제된 GLA를 정량하였다. 마이크로타이터 플레이트에서, 적절하게 희석된 25 ㎕의 단백질 표준물 및 정제된 GLA를, 50 부의 BCA 시약 A 및 1 부의 BCA 시약 B를 함유하는 200 ㎕의 작업 시약과 혼합하였다. 플레이트를 30초 동안 플레이트 진탕기 상에서 철저히 혼합하고 30분 동안 37℃에서 인큐베이션하였다. 플레이트를 실온까지 냉각시킨 후, 플레이트 판독기를 이용하여 샘플의 흡광도를 562 nm에서 측정하였다.
실시예 5
GLA 변이체의 시험관내 특징규명
HEK 293T 세포에서 발현된 GLA 변이체의 열안정성
GLA 변이체를 다양한 온도 챌린지에 노출시켜, 효소의 전체 안정성을 평가하였다. 먼저, 50 ㎕의 1X PBS(pH 6.2) 중의 정제된 HEK 293T 발현 GLA 및 GLA 변이체를 96웰 PCR 플레이트(바이오라드, HSP-9601)의 웰에 첨가하였다. 상기 플레이트를 밀봉하고 열순환기의 구배 프로그램을 이용하여 1시간 동안 30℃ 내지 50℃에서 인큐베이션하였다. 어세이를 위해, 25 ㎕의 챌린지 상청액을 25 ㎕의 McIlvaine 완충제(pH 4.4) 중의 1 mM MUGal과 혼합하였다. 반응물을 짧게 혼합하고 60분 동안 37℃에서 인큐베이션한 후, 100 ㎕의 0.5 M 탄산나트륨(pH 10.2)으로 켄칭하였다. 형광(여기 355 nm, 방출 460 nm)을 모니터링하는 SPECTRAMAX® M2 마이크로플레이트 판독기를 이용하여 가수분해를 분석하였다. 챌린지 샘플의 활성을 비챌린지 샘플의 활성으로 나눔으로써, 퍼센트 잔류 활성을 30℃ 내지 50℃ 범위의 온도에서 1시간 인큐베이션 동안 계산하였는데, 여기서, "비챌린지"는 0시간에서 측정된 가수분해이고, "챌린지"는 각각의 변이체에 대해 특정된 온도에서 1시간에서 측정된 가수분해이다. 이 어세이의 결과는 표 5-1에 나타나 있다. 도 1은 다양한 온도에서 1시간 인큐베이션 후 GLA 변이체의 잔류 활성을 보여주는 그래프를 제공한다.
HEK 293T 세포에서 발현된 GLA 변이체의 혈청 안정성
혈액의 존재 하에서 변이체의 상대적인 안정성을 평가하기 위해, 샘플을 혈청에 노출시켰다. 먼저, 100 ㎕의 1X PBS(pH 6.2) 중의 7.5 ㎍/㎖의 정제된 GLA 변이체 및 90 ㎕의 인간 혈청을 COSTAR® 96웰 환저 플레이트(코닝)의 웰에 첨가하였다. 상기 플레이트를 밀봉하고 0시간 내지 24시간 동안 37℃에서 인큐베이션하였다. 어세이를 위해, 50 ㎕의 챌린지 상청액을 50 ㎕의 McIlvaine 완충제(pH 4.4) 중의 1 mM MUGal과 혼합하였다. 반응물을 짧게 혼합하고 90분 동안 37℃에서 인큐베이션한 후, 100 ㎕의 0.5 M 탄산나트륨(pH 10.2)으로 켄칭하였다. 형광(여기 355 nm, 방출 460 nm)을 모니터링하는 SPECTRAMAX® M2 마이크로플레이트 판독기를 이용하여 가수분해를 분석하였다. 챌린지 샘플의 활성을 비챌린지 샘플의 활성으로 나눔으로써, 24시간 후 혈청에서의 퍼센트 잔류 활성을 계산하였는데, 여기서, "비챌린지"는 0시간에서 측정된 가수분해이고, "챌린지"는 각각의 변이체에 대해 특정된 시점에서 측정된 가수분해이다. 결과는 표 5-1에 나타나 있다. 도 2는 0시간 내지 24시간 동안 인간 혈청으로 챌린지한 후 GLA 변이체의 잔류 활성을 보여주는 그래프를 제공한다.
HEK 293T 세포에서 발현된 GLA 변이체의 라이소좀 안정성
라이소좀 단백질분해효소 및 다른 라이소좀 성분의 존재 하에서 변이체의 상대적인 안정성을 평가하기 위해, 본원에 기재된 바와 같이, 약간 변형된 제조사의 설명서에 따라 GLA 변이체를 인간 라이소좀 용해물(제노텍, #H0610.L)에 노출시켰다. 요약하건대, GLA 변이체를 적절한 농도 범위(0.0625 내지 0.0078125 mM)로 희석하였고, 10 ㎕의 희석액을 COSTAR® 96웰 환저 플레이트(#3798, 코닝)에서 10 ㎕의 2x 이화작용 완충제(제노텍, #K5200) 중의 인간 라이소좀 용해물의 1:20 희석액과 조합하였다. 상기 플레이트를 밀봉하고 0시간 내지 24시간 동안 37℃에서 인큐베이션하였다. 어세이를 위해, 50 ㎕의 챌린지 상청액을 50 ㎕의 McIlvaine 완충제(pH 4.4) 중의 1 mM MUGal과 혼합하였다. 반응물을 짧게 혼합하고 90분 동안 37℃에서 인큐베이션한 후, 100 ㎕의 0.5 M 탄산나트륨(pH 10.2)으로 켄칭하였다. 형광(여기 355 nm, 방출 460 nm)을 모니터링하는 SPECTRAMAX® M2 마이크로플레이트 판독기를 이용하여 가수분해를 분석하였다. 챌린지 샘플의 활성을 비챌린지 샘플의 활성으로 나눔으로써, 24시간 후 라이소좀 추출물에서의 퍼센트 잔류 활성을 계산하였는데, 여기서, "비챌린지"는 0시간에서 측정된 가수분해이고, "챌린지"는 각각의 변이체에 대해 4시간 및 24시간에서 측정된 가수분해이다. 결과는 표 5-1에서 제공되어 있다. 도 3은 0시간 내지 24시간 동안 인간 라이소좀 추출물로 챌린지한 후 GLA 변이체의 잔류 활성을 보여주는 그래프를 제공한다.
HEK293T 세포에서 발현된 정제된 GLA 변이체의 파브리 섬유모세포 내로의 세포 흡수
기준 효소(WT GLA[서열번호 2])에 비해 GLA 변이체의 세포 흡수를 측정하여, 배양된 세포 내로 세포내이입되는 상기 변이체의 전체 능력을 평가하였다. 파브리 섬유모세포(GMO2775, 코리엘 의학 연구소)를, 최소 필수 배지(1% 비필수 아미노산(NEAA; 깁코 #11140-050) 및 15% 소 태아 혈청(코닝 #35-016-CV)으로 보충된 MEM; 깁코 #11095-080)를 함유하는 12웰 배양 접시(VWR, #10861-698)에 시딩하고 전면생장(confluency)까지 생장시켰다(37℃ 및 5% CO2에서 2일 내지 3일). 전면생장에 도달한 후, 보충된 MEM을 멸균 진공으로 제거하고 웰당 1 ㎖의 무혈청 MEM + 1% NEAA로 대체하였다. 실시예 4에 기재된 바와 같이 정제된 효소를 10 ㎍ GLA/㎖로 세포에 첨가하고, 37℃ 및 5% CO2에서 4시간 동안 인큐베이션하였다. 무혈청 배지를 멸균 진공으로 흡인하였고, 세포를 웰당 1 ㎖의 1xPBS로 짧게 세척하였고, PBS를 멸균 진공으로 흡인하였다. 이어서, 세포를 웰당 200 ㎕의 0.25% 트립신-EDTA(VWR #02-0154-0100)로 트립신처리하고 실온에서 약 5분 동안 인큐베이션하여, 플레이트로부터 부착 세포를 떼어내고 남은 세포외 GLA를 분해시켰다. 그 다음, 500 ㎕의 무혈청 MEM을 각각의 웰에 첨가하였고, 샘플을 1.5 ㎖ 미세원심분리 튜브로 옮겼다. 샘플을 5분 동안 8000 RPM에서 원심분리하여 세포를 펠렛화하였다. 배지를 1000 ㎕ 피펫으로 약하게 흡인하였다. 세포 펠렛을 500 ㎕의 1xPBS에 재현탁하고 5분 동안 8000 RPM에서 재펠렛화하였고, PBS를 조심스럽게 제거하였다. 이어서, 100 ㎕ 용해 완충제(1xPBS에 희석된 0.2% TRITON X-100™ 비이온성 계면활성제(시그마 #93443))를 각각의 샘플에 첨가한 후, 1분 내지 2분 동안 초음파처리하고, 4℃에서 10분 동안 12,000 내지 14,000 RPM에서 원심분리하였다. 단백질 및 활성 어세이를 위해 상청액을 멸균 PCR 튜브로 옮겼다. 활성 어세이를 위해, 10 ㎕의 세포 용해 샘플을 50 ㎕의 McIlvaine 완충제(pH 4.6) 중의 2.5 mM MUGal과 혼합하였다. 반응 플레이트를 밀봉하고 60분 동안 37℃에서 인큐베이션한 후, 웰당 140 ㎕의 0.5 M 탄산나트륨(pH 10.2)으로 반응을 켄칭하였다. 형광(여기 355 nm, 방출 460 nm)을 모니터링하는 SPECTRAMAX® M2 마이크로플레이트 판독기를 이용하여 MUGal 가수분해를 측정하였다. 단백질 정량을 위해, 다음과 같이 변형된 제조사의 설명서(피어스, #23225)에 따라 BCA 어세이를 수행하였다: 10 ㎕의 세포 용해 샘플을 190 ㎕의 BCA 작업 시약과 혼합하였고, 플레이트를 밀봉하고 60분 동안 37℃에서 인큐베이션하였다. 흡광도(562nm)를 모니터링하는 SPECTRAMAX® M2 마이크로플레이트 판독기를 이용하여 샘플을 분석하였다. BSA 표준 곡선으로부터 단백질 농도를 계산하였다. 먼저, 효소로 처리된 샘플로부터 비처리된 세포의 배경 비-효소적 형광을 감산한 후 각각의 웰에서의 단백질 농도로 정규화함으로써 각각의 GLA 변이체의 세포 흡수를 계산하였다. 정규화된 GLA 변이체 세포내 활성을 대조군(WT)의 상응하는 활성으로 나눔으로써 세포 흡수 FIOPC를 계산하였다. 도 4는 37℃에서 4시간 동안 인큐베이션한 후, 야생형(서열번호 2)에 비해 상대적인 활성으로서 표현된, 배양된 파브리 환자 섬유모세포 내로의 정제된 GLA 변이체의 세포 흡수의 그래프를 제공한다.
파브리 섬유모세포의 용해물에서의 GLA 활성의 HTP 분석
HTP에서 생성된 GLA 변이체는 세포 내로 흡수되고 24시간 내지 96시간에 걸쳐 활성을 보유하도록 챌린지되었다. 파브리 섬유모세포(GMO2775, 코리엘 의학 연구소)를 플레이팅하고 24시간 내지 72시간에 걸쳐 전면생장까지 생장시켰다. 전면생장에 도달한 후, 자동 BioMek i5 액체 취급 로봇을 이용하여 배지를 제거하였다. 전술된 바와 같이 일시적으로 형질감염된 HEK293T 세포로부터의 컨디셔닝된 배지를 파브리 섬유모세포에 첨가하였고, 세포를 37℃ 및 5% CO2에서 2시간 내지 4시간 동안 GLA 변이체와 함께 인큐베이션하였다. GLA-함유 컨디셔닝된 배지를 자동 BioMek i5 액체 취급 로봇으로 제거하였다. 이어서, 세포를 웰당 150 ㎕의 1xDPBS로 짧게 세척하였고, DPBS를 자동 BioMek i5 액체 취급 로봇으로 제거하였다. 그 다음, 200 ㎕의 완전한 생장 배지를 각각의 웰에 첨가하였고, 플레이트를 24시간 내지 72시간 동안 인큐베이터로 돌려보냈다. 인큐베이션의 종결 시, 완전한 생장 배지를 자동 BioMek i5 액체 취급 로봇으로 제거하였다. 세포를 웰당 150 ㎕의 1xDPBS로 세척하였고, DPBS를 자동 BioMek i5 액체 취급 로봇으로 제거하였다. 0.2% TRITON X-100™ 비이온성 계면활성제(시그마 #93443)로 보충된 50 ㎕의 McIlvaine 완충제(pH 4.4)를 첨가하고 30분 동안 실온에서 교반함으로써 세포를 용해시켰다. 50 ㎕의 McIlvaine 완충제(pH 4.4) 중의 1.5 mM MuGal를 첨가함으로써 활성을 평가하였다. 플레이트를 밀봉하고 400 rpm에서 교반하면서 360분 동안 37℃에서 인큐베이션한 후, 100 ㎕의 0.5 M 탄산나트륨(pH 10.2)으로 켄칭하였다. 형광(여기 355 nm, 방출 460 nm)을 모니터링하는 SPECTRAMAX® M2 마이크로플레이트 판독기를 이용하여 가수분해를 분석하였다. 정규화된 GLA 변이체 세포내 활성을 기준 서열의 상응하는 활성으로 나눔으로써 세포 흡수 FIOPC를 계산하였다.
파브리 섬유모세포에서 GLA에 의해 유도된 글로보트리아오실세라마이드 고갈의 HTP 분석
HTP에서 생성된 GLA 변이체는 세포 내로 흡수되고 글로보트리아오실세라마이드의 세포 로드를 감소시키도록 챌린지되었다. 파브리 섬유모세포(GMO2775, 코리엘 의학 연구소)를 플레이팅하고 24시간 내지 72시간에 걸쳐 전면 생장까지 생장시켰다. 전면생장에 도달한 후, 배지를 자동 BioMek i5 액체 취급 로봇으로 제거하였다. 전술된 바와 같이 일시적으로 형질감염된 HEK293T 세포에 의해 생성된 컨디셔닝된 배지를 섬유모세포에 첨가하고 37℃ 및 5% CO2에서 2시간 내지 4시간 동안 인큐베이션하였다. GLA-함유 컨디셔닝된 배지를 자동 BioMek i5 액체 취급 로봇으로 제거하였다. 이어서, 세포를 웰당 150 ㎕의 1xDPBS로 짧게 세척하였고, DPBS를 자동 BioMek i5 액체 취급 로봇으로 제거하였다. 그 다음, 200 ㎕의 완전한 생장 배지를 각각의 웰에 첨가하였고, 플레이트를 24시간 내지 72시간 동안 인큐베이터로 돌려보냈다. 인큐베이션의 종결 시, 완전한 생장 배지를 자동 BioMek i5 액체 취급 로봇으로 제거하였다. 이어서, 세포를 웰당 150 ㎕의 1xDPBS로 세척하였고, DPBS를 자동 BioMek i5 액체 취급 로봇으로 제거하였다. 글로보트리아오실세라마이드를 약하게 교반하면서 실온에서 30분 동안 10 ng/㎖의 N-헵타데카노일-세라마이드 트리헥소사이드로 보충된 200 ㎕의 메탄올 내로 추출하였다. 밀리포어 소수성 필터 스택을 통해 메탄올 추출물을 환저 96웰 플레이트 내로 여과하였다. 세포 글로보트리아오실세라마이드를 본질적으로 당분야에서 공지되어 있는 바와 같이 LC-MS/MS로 정량하였다(문헌[Provencal et al., Bioanal., 8:1793-1807 [2016]] 참조]. 각각의 세포 샘플에 대한 피크 적분의 합계를 측정하였고, 정규화된 GLA 변이체 글로보트리아오실세라마이드 수준의 변화를 기준 서열로 나눔으로써 글로보트리아오실세라마이드 FIOPC를 계산하였다.
실시예 6
서열번호 58로부터 유도된 GLA 변이체
이 실시예에서, 파브리 섬유모세포에서 GLA 변이체의 활성 및 GLA 변이체에 의한 Gb3 제거를 평가하기 위해 수행된 실험이 기술된다. 이 실시예에서, 서열번호 58을 기준 서열로서 사용하였다(즉, 변이체의 아미노산 차이는 서열번호 58에 비해 표시되어 있고, 어세이 결과는 서열번호 58에 대해 수득된 결과에 비해 보고되어 있다). 이 실험에서, 실시예 5에 기재된 바와 같이, 사전인큐베이션 없이 MU-Gal 활성에 대해 GLA 변이체를 시험하였다. 실시예 5에 기재된 바와 같이, 파브리 섬유모세포에서 Gb3 고갈에 대해서도 변이체를 시험하였다.
실시예 7
서열번호 158로부터 유도된 GLA 변이체
이 실시예에서, GLA 변이체의 활성, pH 7.4에서의 안정성 및 파브리 섬유모세포에서의 세포내 활성을 평가하기 위해 수행된 실험이 기술된다. 이 실시예에서, 기준 서열은 서열번호 158이었다(즉, 변이체의 아미노산 차이는 서열번호 158에 비해 표시되어 있고, 어세이 결과는 서열번호 158에 대한 결과에 비해 보고되어 있다). 실시예 5에 기재된 바와 같이, 사전인큐베이션 없이(비챌린지) GLA MU-Gal 활성에 대해 변이체를 시험하였고, pH 7.4 사전인큐베이션 후 GLA MU-Gal 활성에 대해 변이체를 시험하였다. 실시예 5에 기재된 바와 같이, GLA 변이체와 함께 인큐베이션된 파브리 섬유모세포의 용해 후 MU-Gal 활성에 대해서도 변이체를 시험하였다.
실시예 8
서열번호 372로부터 유도된 GLA 변이체
이 실시예에서, GLA 변이체의 활성, pH 7.4에서의 안정성, Gb3 제거 및 파브리 섬유모세포에서의 세포내 활성을 평가하기 위해 수행된 실험이 기술된다. 이 실시예에서, 기준 서열은 서열번호 372였다(즉, 변이체의 아미노산 차이는 서열번호 372에 비해 표시되어 있고, 어세이 결과는 서열번호 372에 대한 결과에 비해 보고되어 있다). 실시예 5에 기재된 바와 같이, 사전인큐베이션 없이("비챌린지") MU-Gal 활성에 대해 이 변이체를 시험하였고, pH 7.4 사전인큐베이션 후 MU-Gal 활성에 대해 이 변이체를 시험하였다. 실시예 5에 기재된 바와 같이, GLA 변이체와 함께 인큐베이션된 파브리 섬유모세포에서 MU-Gal 활성에 대해서도 변이체를 시험하였다("용해물 FIOPC"). 실시예 5에 기재된 바와 같이, GLA 변이체와 함께 인큐베이션한 후 파브리 섬유모세포에서 Gb3의 고갈에 대해서도 변이체를 시험하였다("Gb3 FIOPC").
실시예 9
서열번호 374로부터 유도된 GLA 변이체
이 실시예에서, 일련의 챌린지 또는 독립적인 챌린지 후 효소 활성을 어세이함으로써 GLA 변이체 활성을 측정하기 위해 수행된 실험이 기술된다. 실시예 5에 기재된 바와 같이, 사전인큐베이션 없이 GLA MU-Gal 활성에 대해 이 변이체를 시험하였고, pH 7.4 사전인큐베이션 후 GLA MU-Gal 활성에 대해 이 변이체를 시험하였다. 실시예 5에 기재된 바와 같이, 변이체와 함께 인큐베이션된 파브리 섬유모세포의 용해 후 MU-Gal 활성에 대해서도 변이체를 시험하였다. 실시예 5에 기재된 바와 같이, 인큐베이션 후 파브리 섬유모세포에서 Gb3 고갈에 대해서도 변이체를 시험하였다.
실시예 10
GLA 변이체의 생체내 특징규명
GLA 변이체를 축적된 Gb3에 대한 그의 활성에 대해 생체내에서 특징규명하였다. 파브리 마우스(5개월령 암컷; 잭슨(Jackson), 스톡 #3535) 및 동일한 유전적 배경을 가진 연령/성별 일치된 야생형 마우스를 사용하였다. 코덱시스(Codexis) 효소 변이체(1.0 mg/kg)를 사용한 단회 IV 주사를 꼬리 정맥을 통해 마우스에게 투여하였다. 예정된 시점(주사 후 1주 및 2주)에서 CO2 마취를 이용하여 동물을 희생시켰고, 질환 관련 조직(예를 들어, 심장 및 신장)을 2개의 부분으로 절개하고(효소 활성 어세이를 위한 한 부분, Gb3 정량을 위한 나머지 한 부분), 드라이 아이스 상에서 냉동시키고 분석할 때까지 -80℃에서 저장하였다. 효소 어세이를 위해, 유리 균질화기 내에서 모터-구동 TEFLON® 코팅된 막자를 이용하여 20x 부피(w/v)의 용해 완충제(1xPBS에 희석된 0.2% TRITON X-100™ 비이온성 계면활성제(시그마 #93443))에서 마우스 조직을 균질화하였다. 용해물을 초음파처리하고 4℃에서 15분 동안 14,000 rpm에서 원심분리하였고, 효소 어세이를 위해 상청액을 사용하였다. 0.1 M N-아세틸갈락토사민(즉, α-갈락토시다제 B의 특이적 억제제)의 존재 하에서 5 mM 4-메틸움벨리페릴-α-D-갈락토피라노사이드(pH 4.4)를 사용한 표준 형광측정 어세이로 α-Gal A 활성을 측정하였다. BCA 단백질 어세이 키트(피어스, #23225)를 사용하여 단백질 농도를 측정하였다. 활성을 단백질 농도로 정규화하고 nmol/mg 단백질/시로서 표현하였다. Gb3 농도를 종래에 기재된 바와 같이 질량 분광측정으로 측정하였다(문헌[Durant et al., J. Lipid Res., 52:1742-6 [2011]] 참조). 요약하건대, 유리 균질화기 내에서 빙냉 20x 부피의 초순수 물에서 마우스 조직을 균질화하였고, 200 ㎍의 총 단백질에 상응하는 용해물을 당스핑고지질 추출, 비누화 및 질량 분광측정에 의한 Gb3의 후속 분석으로 처리하였다. Gb3 농도를 ng/mg 단백질로서 표현하였다. 도 5는 치료한 지 1주, 2주 및 4주 후 파브리 마우스 모델의 심장에서 생체내 효소 활성을 보여주는 그래프를 제공한다. 도 6은 비치료된 동물에 비해 치료한 지 1주 및 2주 후 파브리 마우스 모델의 심장 조직에서 Gb3 분해의 그래프를 제공한다.
실시예 11
서열번호 704로부터 유도된 GLA 변이체
이 실시예에서, 파브리 섬유모세포에서 GLA 변이체에 의한 활성, 혈청 안정성, 파브리 섬유모세포에서의 세포내 활성 및 Gb3 제거를 평가하기 위해 수행된 실험이 기술된다. 이러한 실험에서, GLA 변이체를 실시예 5에 기재된 바와 같이 서열번호 704(서열번호 704는 효모 코돈 최적화된 서열번호 275의 포유동물 코돈 최적화된 버전인 서열번호 703으로부터의 아미노산 서열임)에 비해 파브리 섬유모세포의 용해물에서, 사전인큐베이션 없이, 혈청 인큐베이션 후, MU-Gal 활성에 대하여 시험하였다. 변이체를 또한 실시예 5에 기재된 바와 같이 파브리 섬유모세포에서 Gb3 고갈에 대하여 시험하였다.
실시예 12
서열번호 374로부터 유도된 GLA 변이체
이 실시예에서, 파브리 섬유모세포에서 GLA 변이체에 의한 활성, 혈청 안정성, 파브리 섬유모세포에서의 세포내 활성 및 Gb3 제거를 평가하기 위해 수행된 실험이 기술된다. 이러한 실험에서, GLA 변이체를 실시예 5에 기재된 바와 같이 파브리 섬유모세포의 용해물에서, 사전-인큐베이션 없이, 혈청 인큐베이션 후 MU-Gal 활성에 대하여 시험하였다. 변이체를 또한 실시예 5에 기재된 바와 같이 파브리 섬유모세포에서 Gb3 고갈에 대하여 시험하였다.
실시예 13
서열번호 1022로부터 유도된 GLA 변이체
이 실시예에서, 활성, 혈청 안정성, 및 파브리 섬유모세포에서 세포내 활성을 평가하기 위해 수행된 실험이 기술된다. 이러한 실험에서, GLA 변이체를 실시예 5에 기재된 바와 같이 파브리 섬유모세포의 용해물에서, 사전-인큐베이션 없이, 혈청 인큐베이션 후 MU-Gal 활성에 대하여 시험하였다.
현탁 배양에서 생성된 정제된 GLA 변이체의 혈청 안정성
혈액의 존재에서 변이체의 상대적 안정성을 평가하기 위해, 샘플을 혈청에 노출시켰다. 먼저, 100 ㎕의 1X PBS(pH 6.2) 중의 7.5 ug/㎖의 정제된 GLA 변이체, 및 90 uL의 인간 혈청을 COSTAR® 96-웰 둥근 바닥 플레이트(코닝)의 웰에 첨가하였다. 플레이트를 밀봉하고 37℃에서 0-24h 동안 인큐베이션하였다. 어세이를 위해, 50 ㎕의 챌린지된 상청액을 50 ㎕의 McIlvaine 완충제(pH 4.4) 중의 1 mM MUGal과 혼합하였다. 반응물을 짧게 혼합하고, 37℃에서 90분 동안 인큐베이션한 후, 100 ㎕의 0.5 M 소듐 카르보네이트(pH 10.2)로 켄칭하였다. 형광(여기 355 nm, 방출 460 nm)을 모니터링하는 ENVISION® 마이크로플레이트 리더(퍼킨엘머)를 사용하여 가수분해를 분석하였다. 24h 후 혈청 중 잔류 활성 퍼센트를 챌린지된 샘플의 활성을 비챌린지된 샘플의 활성으로 나눔으로써 계산하였고, 여기서, "비챌린지된"은 시간 0에서 측정된 가수분해이고 "챌린지된"은 각 변이체에 대해 특정 시점에 측정된 가수분해이다. 도 7은 0-24시간 동안 인간 혈청으로의 챌린지 후 GLA 변이체의 잔류 활성을 보여주는 그래프를 제공한다.
현탁 배양에서 생성된 정제된 GLA 변이체의 파브리 섬유모세포에서의 세포 흡수
기준 효소(WT GLA [서열번호 2])와 비교하여 GLA 변이체의 세포 흡수를 확인하여 배양된 세포로 세포내이입되는 변이체의 전반적인 능력을 평가하였다. 파브리 섬유모세포(GMO2775, 코리엘 의학 연구소)를 최소 필수 배지(1% 비필수 아미노산(NEAA; 깁코 #11140-050) 및 15% 소 태아 혈청[코닝 #35-016-CV]이 보충된 MEM; 깁코 #11095-080)를 함유하는 96-웰 배양 접시(VWR, #10861-698)에 시딩하고, 전면생장까지 성장되게 하였다(37℃, 5% CO2에서 1-3일). 전면생장에 도달한 후, 보충된 MEM을 멸균 진공에 의해 제거하고 1 ㎖/웰의 무혈청 MEM + 1% NEAA로 대체하였다. 실시예 4에 기재된 바와 같이 정제된 효소를 220 nM 내지 2 nM의 용량 반응으로 세포에 첨가하고, 37℃, 5% CO2에서 4시간 동안 인큐베이션되게 하였다. 세포를 100 ㎕의 멸균 1xPBS/웰로 2회 세척하고, PBS를 멸균 진공으로 흡인하였다. 이어서, 세포를 최소 필수 배지(1% 비필수 아미노산(NEAA; 깁코 #11140-050) 및 15% 소 태아 혈청[코닝 #35-016-CV]이 보충된 MEM; 깁코 #11095-080)를 함유하여 인큐베이션하고, 37℃, 5% CO2에서 3일 동안 인큐베이션되게 하였다. 3일 후, 세포를 100 ㎕의 멸균 1xPBS/웰로 2회 세척하고, PBS를 멸균 진공으로 흡인하였다. 이후, 25 ㎕의 용해 완충제(1xPBS에 희석된 0.2% TRITON X-100™ 비이온성 계면활성제[시그마 #93443])를 각각의 샘플에 첨가한 다음, 1-2분 동안 초음파 처리하고, 4℃에서 10분 동안 12,000-14,000 RPM으로 원심분리하였다. 활성 어세이를 위해, 25 ㎕의 세포 용해 샘플을 25 ㎕의 McIlvaine 완충제(pH 4.6) 중의 2.5 mM MUGal과 혼합하였다. 반응 플레이트를 밀봉하고, 37℃에서 60분 동안 인큐베이션한 후, 반응을 웰 당 150 ㎕의 0.5 M 소듐 카르보네이트(pH 10.2)로 켄칭하였다. 형광(여기 355 nm, 방출 460 nm)을 모니터링하는 ENVISION® 마이크로플레이트 리더(퍼킨엘머)를 사용하여 MUGal 가수분해를 확인하였다. 도 8은 37℃에서 4시간 인큐베이션 및 3일 세척 후 배양된 파브리 환자 섬유모세포에서 정제된 GLA 변이체의 세포 흡수의 그래프를 제공한다.
실시예 14
GLA 변이체의 생체내 특징규명
이 실시예에서, 일부 GLA 변이체를 특징규명하기 위해 동물 모델을 사용하여 수행된 실험이 기술된다. 마우스에서 약동학적 프로파일을 평가하기 위한 연구 설계의 개요는 표 14-1에 제공된다. 연구는 단일 상으로 수행되었고, 36 마리의 수컷 C57Bl/6 마우스(20 - 25g)를 포함하였다. 동물을 실험 전 적어도 3일 동안 순응시켰다. 모든 동물들은 WT GLA(서열번호 2) 또는 GLA 변이체를 꼬리 정맥을 통해 1 mg/kg 단회 IV 주사를 받았다. 개별 용량은 투여일에 얻어진 체중에 기초하여 계산하였다.
미리 결정된 시점에, 약 150 ㎕의 전혈을 시험 항목 당 6 마리의 마우스로부터 헤파린화된 모세관으로 수집하고(표 14-1에 따라 각 시점에 대해 6 마리의 2개 그룹 사이에 교대로), 혈장에 대해 즉시 처리하고, 어세이될 때까지 -80℃에서 저장하였다. 실시예 5에 기재된 바와 같이, 표준 형광측정 MuGal 어세이를 이용하여 혈장 GLA 활성을 측정하였다. 전반적으로, GLA 변이체는 서열번호 2와 비교하여 우수한 혈장 약동학적 프로파일을 나타냈고, 후자는 약 10분의 추정된 반감기(t1/2)로 가장 빠른 제거를 가졌다. 이에 비해, GLA 변이체에 대한 t1/2는 대략 10배 더 높았다. 데이터는 표 14-2에 제공되어 있다.
상기 기재된 마우스 연구에 추가하여, 건강한 래트에서 일부 GLA 변이체의 약동학을 확인하기 위해 실험을 수행하였다. 간략한 연구 설계는 표 14-3에 나타나 있다. 첫 번째 연구는 3 상으로 수행되었고 21 마리의 래트를 포함하였다. 동물을 실험 전 적어도 3일 동안 순응시켰다. 모든 동물들은 WT GLA(서열번호 2) 또는 GLA 변이체의 경정맥 캐뉼러를 통해 IV 주사를 받았다. 개별 용량은 투여일에 얻어진 체중에 기초하여 계산하였다.
혈액(약 0.25 ㎖)을 표 14-3에 나타낸 미리 결정된 시점에 각 래트로부터 EDTA 분리기 튜브에 수집하고, 혈장에 대해 즉시 처리하고, 어세이될 때까지 -80℃에서 저장하였다. 실시예 5에 기재된 바와 같이, 표준 형광측정 MuGal 어세이를 이용하여 혈장 GLA 활성을 측정하였다. 모든 GLA 변이체는 서열번호 2와 비교할 때 개선된 PK 성질을 나타냈다. 모든 PK 파라미터에 대한 데이터는 표 14-4에 제공되어 있다.
래트 PK 연구 번호 2에 대한 간략한 연구 설계가 표 14-5에 제공되어 있다. 연구는 1 상으로 수행되었고 18 마리의 래트를 포함하였다. 동물을 실험 전 적어도 3일 동안 순응시켰다. 모든 동물들은 WT GLA(서열번호 2) 또는 GLA 변이체의 경정맥 캐뉼러를 통해 IV 주사를 받았다. 개별 용량은 투여일에 얻어진 체중에 기초하여 계산하였다.
혈액(약 0.25 ㎖)을 표 14-5에 나타낸 미리 결정된 시점에 EDTA 분리기 튜브에 수집하고, 혈장에 대해 즉시 처리하고, 어세이될 때까지 -80℃에서 저장하였다. 실시예 5에 기재된 바와 같이, 표준 형광측정 MuGal 어세이를 이용하여 혈장 GLA 활성을 측정하였다. 모든 용량의 GLA 변이체는 서열번호 2와 비교할 때 개선된 PK 성질을 야기하였다. 모든 PK 파라미터에 대한 데이터는 표 14-6에 제공되어 있다.
상기 기재된 마우스 및 래트 연구에 추가하여, 영장류 모델에서 실험을 수행하였다. 간략한 연구 설계는 표 14-7에 나타나 있다. 연구는 1 상으로 수행되었고 12 마리의 수컷 사이노몰구스 원숭이(2 - 3 kg)를 포함하였다. 동물들은 시험 시설의 콜로니로부터 공급되었고 단백질 나이브(naive)로 간주되었다. 모든 동물들은 WT GLA(서열번호 2) 또는 GLA 변이체의 IV 주사를 받았다. 개별 용량은 투여일에 얻어진 체중에 기초하여 계산하였다.
혈액(약 0.5 ㎖)을 표 14-7에 나타낸 미리 결정된 시점에 EDTA 분리기 튜브에 수집하고, 혈장에 대해 즉시 처리하고, 어세이될 때까지 -80℃에서 저장하였다. 실시예 5에 기재된 바와 같이, 표준 형광측정 MuGal 어세이를 이용하여 혈장 GLA 활성을 측정하였다. 모든 용량의 GLA 변이체는 서열번호 2와 비교할 때 개선된 PK 성질을 야기하였다. 모든 PK 파라미터에 대한 데이터는 표 14-8에 제공되어 있다.
원숭이에서 제2 PK 연구에 대한 간략한 연구 설계가 표 14-9에 나타나 있다. 연구는 1 상으로 수행되었고 12 마리의 사이노몰구스 원숭이(3마리의 동물/그룹)를 포함하였다. 동물들은 시험 시설의 콜로니로부터 공급되었고 GLA 단백질 나이브로 간주되었다. 모든 동물들은 WT GLA(서열번호 2) 또는 GLA 변이체의 IV 주사를 받았다. 개별 용량은 투여일에 얻어진 체중에 기초하여 계산하였다. 혈액 샘플은 다음 시점에 채취하였다: 투여 전(최대 3시간), 투여 후 1분, 5분, 15분, 30분, 1시간, 2시간, 4시간, 8시간, 12시간 및 24시간.
혈액(약 1 ㎖)을 표 14-7에 나타낸 미리 결정된 시점에 EDTA 분리기 튜브에 수집하고, 혈장에 대해 즉시 처암컷;리하고, 어세이될 때까지 -80℃에서 저장하였다. 실시예 5에 기재된 바와 같이, 표준 형광측정 MuGal 어세이를 이용하여 혈장 GLA 활성을 측정하였다. 모든 용량의 GLA 변이체는 서열번호 2와 비교할 때 개선된 PK 성질을 야기하였다. 모든 PK 파라미터에 대한 데이터는 표 14-10에 제공되어 있다.
파브리 넉아웃 마우스에서 rhGLA와 비교한 GLA 변이체의 효능
파브리 마우스(5개월령 잭슨, 스톡 #3535) 및 동일한 유전적 배경을 가지는 연령/성별 매칭된 야생형 마우스를 사용하여 WT GLA(서열번호 2)와 비교하여 6개의 GLA 변이체(서열번호 4, 58, 158, 704, 1022, 및 1864)의 효능을 평가하였다. 마우스에 4주 동안 주 1회 효소 변이체(0.1 mg/kg, 0.3 mg/kg, 또는 1.0 mg/kg, 꼬리 정맥 주사를 통해)를 투여하였다(n = 5/그룹). 마지막 주사 7일 후에, 동물을 마취시켜 K3 EDTA 튜브에서 혈액 수집을 위해 심장 천자를 수행한 다음, 안락사시켰다. 조직으로부터 오염 혈액을 제거하기 위해 차가운 식염수로 심장을 통한 전신 관류를 수행하였다. 질환 관련 조직(예를 들어, 심장 및 신장)을 2개의 부분으로 해부하고(효소 활성 어세이를 위한 한 부분, Gb3 및 lyso-Gb3 정량을 위한 나머지 한 부분), 드라이 아이스에서 동결시키고, 분석할 때까지 -80℃에서 저장하였다. 혈액을 혈장에 대해 즉시 처리하고, 어세이될 때까지 -80℃에서 저장하였다. 실시예 5에 기재된 바와 같이, 표준 형광측정 MuGal 어세이를 이용하여 혈장 및 조직 GLA 활성을 측정하였다. 혈장 및 조직 Gb3 및 lyso Gb3 수준을 실시예 10에 언급된 방법을 이용하여 평가하고, 조직을 실시예 10에 기재된 바와 같이 균질화하였다. 서열번호 1864의 일부 경우를 제외하고, 모든 GLA 변이체는 파브리 마우스 모델에서 서열번호 2보다 더 효능이 있었다.
도 9 및 10은 마지막 처리 7일 후에 파브리 마우스 모델에서 각각 심장 및 신장에서의 생체내 효소 활성을 보여주는 그래프를 제공한다. 도 9에서, 데이터는 평균 ± SEM으로 표시된다. Dunnett의 사후 검정과 이원 ANOVA를 이용하여 결과를 서열번호 2의 결과와 비교하였고; "∧", p<0.0001, 서열번호 2에 비해 만족스럽지 않은 유의한 개선은 이 도면에 도시되어 있지 않다. 도 10에서, 데이터는 평균 ± SEM으로 표시된다. Dunnett의 사후 검정과 이원 ANOVA를 이용하여 결과를 서열번호 2의 결과와 비교하였고; "*" p<0.05; "**" p<0.005; 및 "∧", p<0.0001, 서열번호 2에 비해 만족스럽지 않은 유의한 개선은 이 도면에 도시되어 있지 않다. 도 11 및 12는 각각 심장 및 신장 조직에서 Gb3 분해의 그래프를 제공한다. 도 11에서, 데이터는 평균 ± SEM으로 표시된다. Dunnett의 사후 검정과 이원 ANOVA를 이용하여 결과를 서열번호 2의 결과와 비교하였고; 0.1 mg/kg 용량에서, "*", 서열번호 158 및 1022에 대해 p<0.05; 및 0.3 mg/kg 용량에서, "∧", 서열번호 158에 대해 p<0.0001; "**", SEQ ID NOS: 58, 704, 1022, 및 1864에 대해 p<0.005. 도 12에서, 데이터는 평균 ± SEM으로 표시된다. Dunnett의 사후 검정과 이원 ANOVA를 이용하여 결과를 서열번호 2의 결과와 비교하였고; 0.1 mg/kg 용량에서, "∧", 서열번호 4, 58, 158, 704, 및 1022에 대해 p<0.0001; 및 0.3 mg/kg 용량에서, "∧", 서열번호 158에 대해 p<0.0001; "**", 서열번호 4, 58, 704, 및 1022에 대해 p<0.005. 도 13 및 14는 각각 심장 및 신장 조직에서 lyso-Gb3 분해의 그래프를 제공한다. 도 13에서, 데이터는 평균 ± SEM으로 표시된다. Dunnett의 사후 검정과 이원 ANOVA를 이용하여 결과를 서열번호 2의 결과와 비교하였다. 도 14에서, 데이터는 평균 ± SEM으로 표시된다. Dunnett의 사후 검정과 이원 ANOVA를 이용하여 결과를 서열번호 2의 결과와 비교하였다; 0.1 mg/kg 용량에서, "**", 서열번호 4, 58, 158에 대해 p<0.005; "*", 서열번호 704에 대해 p<0.05.
본 발명은 구체적인 실시양태를 참조로 하여 기술되었지만, 특정 상황, 물질, 물질 조성, 프로세스, 프로세스 단계 또는 단계들에 맞게 적합화시키기 위해 다양하게 변형될 수 있고, 등가물로 치환될 수 있고, 이로써, 청구되는 범주로부터 벗어남 없이 본 발명의 이점을 달성할 수 있다.
미국에서의 모든 목적을 위해, 본 개시에서 인용되는 각각의 모든 공개문헌 및 특허 문헌은 마치 각각의 상기 공개문헌 또는 문헌이 본원에서 참조로 포함되는 것으로 구체적으로 및 개별적으로 명시된 바와 같이 본원에서 참조로 포함된다. 공개문헌 및 특허 문헌을 인용하는 것은 임의의 상기 문헌이 관련된 선행 기술임을 나타내는 것으로 의도되는 것은 아니며, 그의 내용 또는 일자와 관련해서 인정하는 것도 아니다.
SEQUENCE LISTING
<110> CODEXIS, INC.
<120> HUMAN ALPHA-GALACTOSIDASE VARIANTS
<130> CX7-203WO2
<140> PCT/US2021/019811
<141> 2021-02-26
<150> US 62/982,949
<151> 2020-02-28
<160> 1864
<210> 1
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> wild type human alpha-galactosidase A
<400> 1
ttggataacg ggttagcccg tacacctact atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcatggag 120
atggctgaac taatggtaag tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aactacgtac acagcaaggg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggagcat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catggaagtc aatcaaatct atcttggatt ggacttcttt caaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggaa tcaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
gcacccctat tcatgtctaa tgatctacgt cacatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agacaaggtg acaactttga agtgtgggaa agaccattgt ctggacttgc gtgggctgtt 960
gctatgatca accgtcaaga gatcggaggg ccaagatctt acactatcgc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaagttgg gtttctatga gtggacatct aggctaagaa gtcacatcaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaatgtctt tgaaagattt gtta 1194
<210> 2
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> wild type human alpha-galactosidase A
<400> 2
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val
305 310 315 320
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu
385 390 395
<210> 3
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 3
ttggataacg ggttagcccg tacacctact atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcatggag 120
atggctgaac ggatggtaag tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aactacgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
gcacccctat tcatgtctaa tgatctacgt cacatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagatgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agacaattgg gtttctatga atggacctct aggctaaaaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaatgtctt tgaaagattt gtta 1194
<210> 4
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 4
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Arg Met Val Ser Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Gln Leu Gly Phe Tyr Glu Trp
355 360 365
Thr Ser Arg Leu Lys Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu
385 390 395
<210> 5
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 5
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagatgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agacaattgg gtttctataa cgcaacctct aggctaaaaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gtta 1194
<210> 6
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 6
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Gln Leu Gly Phe Tyr Asn Ala
355 360 365
Thr Ser Arg Leu Lys Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 7
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 7
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagatgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agacaattgg gtttctatga agcaacctct aggctaaaaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gtta 1194
<210> 8
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 8
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Gln Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Lys Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 9
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 9
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac tgatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg caaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggaa ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatcgc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agacaattgg gtttctatga agcaacctct aggctaaaaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gtta 1194
<210> 10
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 10
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Gln Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Lys Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 11
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 11
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac tgatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agacaaggtg acaactttga agtgtgggaa agaccattgt ctggagatgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agacaattgg gtttctatga agcaacctct aggctaaaaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gtta 1194
<210> 12
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 12
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Gln Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Lys Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 13
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 13
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac tgatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggaa ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agacaaggtg acaactttga agtgtgggaa agaccattgt ctggagatgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agacaattgg gtttctatga agcaacctct aggctaaaaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gtta 1194
<210> 14
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 14
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Gln Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Lys Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 15
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 15
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttcttt taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagatgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agacaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gtta 1194
<210> 16
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 16
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Gln Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 17
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 17
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagatgc gtgggctgtt 960
gctatgatca accgtcaaga gatcggaggg ccaagatctt acactatcgc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agacaattgg gtttctatga agcaacctct aggctaaaaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gtta 1194
<210> 18
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 18
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Gln Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Lys Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 19
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 19
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac tgatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggaa ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agacaaggtg acaactttga agtgtgggaa agaccattgt ctggagatgc gtgggctgtt 960
gctatgatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agacaattgg gtttctatga agcaacctct aggctaaaaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gtta 1194
<210> 20
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 20
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Gln Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Lys Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 21
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 21
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agacaaggtg acaactttga agtgtgggaa agaccattgt ctggagatgc gtgggctgtt 960
gctatgatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gtta 1194
<210> 22
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 22
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 23
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 23
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagatgc gtgggctgtt 960
gctatgatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agacaattgg gtttctatga agcaacctct aggctaaaaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gtta 1194
<210> 24
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 24
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Gln Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Lys Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 25
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 25
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agacaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatcgc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agacaattgg gtttctatga agcaacctct aggctaaaaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gtta 1194
<210> 26
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 26
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Gln Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Lys Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 27
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 27
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac tgatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttcttt taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagatgc gtgggctgtt 960
gctatgatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agacaattgg gtttctatga agcaacctct aggctaaaaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gtta 1194
<210> 28
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 28
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Gln Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Lys Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 29
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 29
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac tgatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggaa ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagatgc gtgggctgtt 960
gctatgatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agacaattgg gtttctatga agcaacctct aggctaaaaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gtta 1194
<210> 30
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 30
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Gln Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Lys Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 31
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 31
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac tgatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg caaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatcgc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agacaattgg gtttctatga agcaacctct aggctaaaaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gtta 1194
<210> 32
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 32
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Gln Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Lys Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 33
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 33
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac tgatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agacaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gtta 1194
<210> 34
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 34
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Gln Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 35
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 35
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac tgatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggaa ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagatgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agacaattgg gtttctatga agcaacctct aggctaaaaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gtta 1194
<210> 36
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 36
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Gln Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Lys Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 37
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 37
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttcttt taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagatgc gtgggctgtt 960
gctatgatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agacaattgg gtttctatga agcaacctct aggctaaaaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gtta 1194
<210> 38
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 38
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Gln Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Lys Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 39
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 39
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac tgatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttcttt taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggattagc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agacaattgg gtttctatga agcaacctct aggctaaaaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gtta 1194
<210> 40
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 40
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Gln Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Lys Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 41
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 41
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggaa ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagatgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaaaaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gtta 1194
<210> 42
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 42
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Lys Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 43
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 43
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac tgatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttcttt taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagatgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agacaattgg gtttctatga agcaacctct aggctaaaaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gtta 1194
<210> 44
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 44
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Gln Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Lys Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 45
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 45
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagatgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agacaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gtta 1194
<210> 46
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 46
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Gln Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 47
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 47
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggaa ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctatgatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agacaattgg gtttctatga agcaacctct aggctaaaaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gtta 1194
<210> 48
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 48
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Gln Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Lys Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 49
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 49
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac tgatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagatgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agacaattgg gtttctatga agcaacctct aggctaaaaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gtta 1194
<210> 50
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 50
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Gln Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Lys Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 51
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 51
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggattagc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agacaattgg gtttctatga agcaacctct aggctaaaaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gtta 1194
<210> 52
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 52
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Gln Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Lys Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 53
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 53
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac tgatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggaa ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagatgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaaaaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gtta 1194
<210> 54
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 54
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Lys Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 55
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 55
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggattagc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatcgc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agacaattgg gtttctatga agcaacctct aggctaaaaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gtta 1194
<210> 56
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 56
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Gln Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Lys Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 57
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 57
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gtta 1194
<210> 58
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 58
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 59
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 59
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac tgatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttcttt taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagatgc gtgggctgtt 960
gctatgatca accgtcaaga gatcggaggg ccaagatctt acactatcgc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agacaattgg gtttctatga agcaacctct aggctaaaaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gtta 1194
<210> 60
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 60
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Gln Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Lys Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 61
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 61
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtagggcgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagatgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaaaaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gtta 1194
<210> 62
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 62
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Ala Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Lys Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 63
<211> 100
<212> DNA
<213> Artificial Sequence
<220>
<223> BamHI-pCDNA-GLA-F
<400> 63
acgtttggat cccgccacca tggaatggag ctgggtcttt ctcttcttcc tgtcagtaac 60
gactggtgtc cactccttgg ataacgggtt agcccgtaca 100
<210> 64
<211> 35
<212> DNA
<213> Artificial Sequence
<220>
<223> XhoI-pCDNA-GLA-R
<400> 64
attgtactcg agttataaca aatctttcaa agaca 35
<210> 65
<211> 57
<212> DNA
<213> Artificial Sequence
<220>
<223> synthetic mouse IG signal peptide
<400> 65
atggaatgga gctgggtctt tctcttcttc ctgtcagtaa cgactggtgt ccactcc 57
<210> 66
<211> 19
<212> PRT
<213> Artificial Sequence
<220>
<223> synthetic mouse IG signal peptide
<400> 66
Met Glu Trp Ser Trp Val Phe Leu Phe Phe Leu Ser Val Thr Thr Gly
1 5 10 15
Val His Ser
<210> 67
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 67
ttggataacg ggttagccct gacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc atttggtcac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagacaagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 68
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 68
Leu Asp Asn Gly Leu Ala Leu Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly His Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 69
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 69
ttggataacg ggttagccct gacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagacaagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtgtctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 70
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 70
Leu Asp Asn Gly Leu Ala Leu Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Val Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 71
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 71
ttggataacg ggttagccct gacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tgcccttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc atttggtcac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagacaagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acgggtttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtgtctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 72
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 72
Leu Asp Asn Gly Leu Ala Leu Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Ala Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly His Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Gly Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Val Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 73
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 73
ttggataacg ggttagccct gacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acgggtttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 74
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 74
Leu Asp Asn Gly Leu Ala Leu Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Gly Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 75
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 75
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tgcccttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc atttggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 76
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 76
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Ala Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 77
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 77
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caaaacaagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaattttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 78
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 78
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asn Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 79
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 79
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcgtatc 900
agaaaaggtg acaattttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 80
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 80
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Arg Ile Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 81
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 81
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc atttggtcac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagacaagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 82
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 82
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly His Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 83
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 83
ttggataacg ggttagccct gacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagacaagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 84
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 84
Leu Asp Asn Gly Leu Ala Leu Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 85
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 85
ttggataacg ggttagccct gacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acgggtttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtgtctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 86
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 86
Leu Asp Asn Gly Leu Ala Leu Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Gly Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Val Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 87
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 87
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc atttggtcac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcgtctg 900
agaaaaggtg acgggtttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 88
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 88
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly His Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Arg Leu Arg Lys Gly Asp
290 295 300
Gly Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 89
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 89
ttggataacg ggttagccct gacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgatggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc agagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc atttggtcac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attcgctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caaaacaagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcgtctg 900
agaaaaggtg acaattttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 90
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 90
Leu Asp Asn Gly Leu Ala Leu Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Glu Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly His Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asn Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Arg Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 91
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 91
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgatggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 92
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 92
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 93
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 93
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc agagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 94
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 94
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Glu Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 95
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 95
ttggataacg ggttagccct gacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgag tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 96
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 96
Leu Asp Asn Gly Leu Ala Leu Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Glu Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 97
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 97
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctac acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attcgctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgggt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 98
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 98
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Thr Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Gly Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 99
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 99
ttggataacg ggttagccct gacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgatggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attcgctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgggt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 100
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 100
Leu Asp Asn Gly Leu Ala Leu Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Gly Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 101
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 101
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagacaagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtgtctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 102
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 102
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Val Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 103
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 103
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attcgctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagacaagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaaatc 900
agaaaaggtg acaattttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 104
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 104
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Ile Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 105
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 105
ttggataacg ggttagccct gacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgatggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc agagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 106
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 106
Leu Asp Asn Gly Leu Ala Leu Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Glu Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 107
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 107
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgatggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc atttggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attcgctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgggt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caaaacaagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 108
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 108
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Gly Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asn Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 109
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 109
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc atttggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgggt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 110
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 110
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Gly Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 111
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 111
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcgtatc 900
agaaaaggtg acgggtttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtgtctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 112
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 112
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Arg Ile Arg Lys Gly Asp
290 295 300
Gly Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Val Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 113
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 113
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caaaacaagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtgtctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 114
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 114
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asn Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Val Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 115
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 115
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgatggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caaaacaagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaactg 900
agaaaaggtg acgggtttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 116
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 116
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asn Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Gly Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 117
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 117
ttggataacg ggttagccct gacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc agagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgggt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 118
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 118
Leu Asp Asn Gly Leu Ala Leu Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Glu Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Gly Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 119
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 119
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagacaagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaactg 900
agaaaaggtg acgggtttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 120
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 120
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Gly Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 121
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 121
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc agagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attcgctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcgtatc 900
agaaaaggtg acaattttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 122
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 122
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Glu Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Arg Ile Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 123
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 123
ttggataacg ggttagccct gacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgatggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgag tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attcgctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caaaacaagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 124
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 124
Leu Asp Asn Gly Leu Ala Leu Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Glu Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asn Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 125
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 125
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgatggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc agagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 126
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 126
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Glu Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 127
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 127
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagacaagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acgggtttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtgtctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 128
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 128
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Gly Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Val Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 129
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 129
ttggataacg ggttagccct gacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caaaacaagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 130
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 130
Leu Asp Asn Gly Leu Ala Leu Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asn Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 131
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 131
ttggataacg ggttagccct gacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc agagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgag tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caaaacaagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtgtctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 132
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 132
Leu Asp Asn Gly Leu Ala Leu Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Glu Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Glu Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asn Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Val Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 133
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 133
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgatggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caaaacaagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaactg 900
agaaaaggtg acaattttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 134
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 134
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asn Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 135
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 135
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catggtcgtc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaaag gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 136
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 136
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ser Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 137
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 137
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aaccgtactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcgtc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtgacg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 138
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 138
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Asp Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 139
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 139
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtggcg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 140
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 140
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Gly Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 141
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 141
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg ccagcttgca aatcatgtac acagtctggg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 142
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 142
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Leu
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 143
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 143
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagtctggg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agacaattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtggcg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 144
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 144
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Leu
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Gln Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Gly Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 145
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 145
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aatcatgtac acagtctggg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcgtc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataccgc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaaag gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 146
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 146
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Leu
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Pro Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 147
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 147
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tgcagctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcgtc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataggcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaaag gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 148
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 148
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Ala Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Gly Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 149
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 149
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tcgtgctcta 480
aaccgtactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcgtc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 150
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 150
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Arg Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 151
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 151
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg ccagcttgca cagcatgtac acgagaaggg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcgtc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaaag gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 152
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 152
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Gln His Val His Glu Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 153
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 153
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg ccagcttgca cagcatgtac acgagctggg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcgtc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaaag gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 154
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 154
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Gln His Val His Glu Leu
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 155
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 155
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aaccatactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtgacg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 156
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 156
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn His Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Asp Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 157
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 157
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 158
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 158
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 159
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 159
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataccgc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaaag gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 160
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 160
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Pro Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 161
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 161
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacagtactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 162
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 162
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Ser Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 163
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 163
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcataaa gcagcttgca aatcatgtac acagtattgg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aaaacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcgtc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataccgc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtggcg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 164
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 164
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Lys Gln Leu Ala Asn His Val His Ser Ile
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys Asn Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Pro Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Gly Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 165
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 165
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcataaa gcagcttgca cagcatgtac acgagctggg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catggtcgtc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtggcg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 166
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 166
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Lys Gln Leu Ala Gln His Val His Glu Leu
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ser Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Gly Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 167
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 167
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcataaa gcagcttgca cagcatgtac acgagctggg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tgcagctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 168
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 168
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Lys Gln Leu Ala Gln His Val His Glu Leu
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Ala Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 169
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 169
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcgtc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataccgc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 170
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 170
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Pro Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 171
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 171
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataccgc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtggcg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 172
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 172
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Pro Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Gly Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 173
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 173
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaga cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcgtc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaaag gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 174
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 174
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Asp Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 175
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 175
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaga cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcgtc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtggcg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 176
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 176
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Asp Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Gly Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 177
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 177
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaga cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcataaa gcagcttgca aatcatgtac acgagctggg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tcgtgctcta 480
aaccatactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcgtc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 178
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 178
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Asp Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Lys Gln Leu Ala Asn His Val His Glu Leu
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Arg Ala Leu
145 150 155 160
Asn His Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 179
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 179
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaga cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataccgc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 180
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 180
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Asp Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Pro Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 181
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 181
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaga cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg ccagcttgca aatcatgtac acgagaaggg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aaccgtactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaaag gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 182
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 182
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Asp Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Glu Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 183
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 183
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat ggggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 184
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 184
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Gly Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 185
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 185
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacaagatg caaacctctt tgaaagattt gttataa 1197
<210> 186
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 186
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Lys Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 187
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 187
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgacg 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 188
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 188
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Thr Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 189
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 189
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtgcgtatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 190
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 190
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Ala Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 191
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 191
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccggtt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 192
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 192
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Val Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 193
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 193
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tgcggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 194
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 194
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Ala Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 195
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 195
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgctg 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 196
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 196
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Leu Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 197
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 197
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaaccggt aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 198
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 198
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Gly Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 199
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 199
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gtcttaa 1197
<210> 200
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 200
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Ser
385 390 395
<210> 201
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 201
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagatgc tttataa 1197
<210> 202
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 202
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Ala Leu
385 390 395
<210> 203
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 203
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catggaagtc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 204
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 204
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 205
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 205
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggaagat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 206
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 206
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Lys Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 207
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 207
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt aggatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 208
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 208
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Arg Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 209
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 209
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaaggttt aactcga 1197
<210> 210
<211> 399
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 210
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Gly Leu Thr Arg
385 390 395
<210> 211
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 211
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gagtccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 212
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 212
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Ser Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 213
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 213
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgg cgggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 214
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 214
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ala Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 215
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 215
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat ccggaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 216
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 216
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Pro Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 217
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 217
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctcta agaaagattt gttataa 1197
<210> 218
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 218
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Lys Lys Asp Leu Leu
385 390 395
<210> 219
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 219
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
gctaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 220
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 220
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Ala Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 221
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 221
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgcggcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 222
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 222
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Ala Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 223
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 223
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatattc acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 224
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 224
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Ile His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 225
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 225
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtcagtatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 226
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 226
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Gln Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 227
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 227
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactgtgcc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 228
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 228
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Val
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 229
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 229
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aactttgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 230
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 230
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Phe Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 231
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 231
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt ggtgtaa 1197
<210> 232
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 232
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Val
385 390 395
<210> 233
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 233
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gccgtaa 1197
<210> 234
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 234
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Pro
385 390 395
<210> 235
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 235
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
atgaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 236
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 236
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Met Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 237
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 237
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccg ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 238
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 238
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Arg Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 239
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 239
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagagggt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 240
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 240
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 241
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 241
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaacagg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 242
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 242
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Gln Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 243
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 243
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtagt ggaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 244
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 244
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Val Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 245
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 245
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aacacggtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 246
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 246
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Thr Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 247
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 247
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaaccgttt tgaaagattt gttataa 1197
<210> 248
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 248
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Val Leu Lys Asp Leu Leu
385 390 395
<210> 249
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 249
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc ctggggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 250
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 250
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Leu Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 251
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 251
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggt 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatttt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 252
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 252
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Phe Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 253
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 253
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcctg gtaaacaagg ttatcaatta 900
agaaagggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg gctacctctt tgaaagattt gttataa 1197
<210> 254
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 254
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Pro Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Ala Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 255
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 255
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtcaggcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 256
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 256
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Gln Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 257
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 257
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgtggag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 258
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 258
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Val Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 259
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 259
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctaa gaaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 260
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 260
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Lys Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 261
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 261
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt ggcttaa 1197
<210> 262
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 262
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Ala
385 390 395
<210> 263
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 263
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgacggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 264
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 264
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 265
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 265
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgacggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc agagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 266
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 266
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Glu Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 267
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 267
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgacggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc agagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctaa gaaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagattct acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg gcgaccgtat tgaaagattt gttataa 1197
<210> 268
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 268
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Glu Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Lys Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Phe Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Ala Thr Val Leu Lys Asp Leu Leu
385 390 395
<210> 269
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 269
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgacggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc agagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctctgg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagattct acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaactgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 270
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 270
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Glu Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Phe Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 271
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 271
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgacggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctaa gaaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagaagtt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 272
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 272
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Lys Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 273
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 273
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgacggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagattct acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 274
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 274
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Phe Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 275
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 275
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgacggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagaggtt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 276
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 276
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 277
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 277
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgacggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagaagtt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg cagaccgtat tgaaagattt gttataa 1197
<210> 278
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 278
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Val Leu Lys Asp Leu Leu
385 390 395
<210> 279
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 279
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgacggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtcaagcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg cagaccgtat tgaaagattt gttataa 1197
<210> 280
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 280
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Gln Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Val Leu Lys Asp Leu Leu
385 390 395
<210> 281
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 281
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc agagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 282
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 282
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Glu Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 283
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 283
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc agagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtcaagcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 284
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 284
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Glu Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Gln Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 285
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 285
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctaa gaaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagattct acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 286
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 286
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Lys Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Phe Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 287
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 287
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctaa gaaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagaggtt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg cagaccagct tgaaagattt gttataa 1197
<210> 288
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 288
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Lys Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 289
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 289
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagattct acactatccc ggtagcctct 1020
ttgggtaagg gtcaagcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 290
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 290
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Phe Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Gln Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 291
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 291
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagaggtt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 292
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 292
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 293
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 293
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgag tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 294
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 294
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Glu Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 295
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 295
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaggag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc ctggggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catggtcgtc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caaaacaagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
cgtaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccg tgtagcctct 1020
ttgggtgatg gtcaggcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaaccagct tgaaagattt ggcgtaa 1197
<210> 296
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 296
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Leu Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ser Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asn Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Arg Val Ala Ser Leu Gly Asp Gly Gln Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Ala
385 390 395
<210> 297
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 297
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaggag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgag tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcgtc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc tgtagcctct 1020
ttgggtaagg gtcaggcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaaccgtat tgaaagattt gctgtaa 1197
<210> 298
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 298
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Glu Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Gln Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Val Leu Lys Asp Leu Leu
385 390 395
<210> 299
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 299
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgtggag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcgtc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccg tgtagcctct 1020
ttgggtggtg gtgtcgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt ggcgtaa 1197
<210> 300
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 300
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Val Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Arg Val Ala Ser Leu Gly Gly Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Ala
385 390 395
<210> 301
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 301
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtagt tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc ctggggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
cgtaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 302
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 302
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Val Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Leu Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 303
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 303
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtagt tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgag tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 304
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 304
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Val Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Glu Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 305
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 305
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtagt tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtgatg gtcaggcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaaccgtat tgaaagattt gctgtaa 1197
<210> 306
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 306
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Val Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Asp Gly Gln Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Val Leu Lys Asp Leu Leu
385 390 395
<210> 307
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 307
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaggag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catggtcgtc aatcaaatct atcttggatt ggacttctaa gaaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
cgtaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 308
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 308
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ser Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Lys Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 309
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 309
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccg tgtagcctct 1020
ttgggtggtg gtcaggcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaaccagct tgaaagattt ggcgtaa 1197
<210> 310
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 310
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Arg Val Ala Ser Leu Gly Gly Gly Gln Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Ala
385 390 395
<210> 311
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 311
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caaaacaagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaaccgtat tgaaagattt gctgtaa 1197
<210> 312
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 312
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asn Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Val Leu Lys Asp Leu Leu
385 390 395
<210> 313
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 313
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcgtc aatcaaatct atcttggatt ggacttctcg caaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtgatg gtgtcgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 314
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 314
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Asp Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 315
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 315
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg caaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtgatg gtcaggcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaaccgtat tgaaagattt ggcgtaa 1197
<210> 316
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 316
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Asp Gly Gln Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Val Leu Lys Asp Leu Ala
385 390 395
<210> 317
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 317
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgtggag 120
atggctgaac ggatggtagt tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctaa gaaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaaccagct tgaaagattt gctgtaa 1197
<210> 318
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 318
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Val Glu Met Ala Glu Arg Met Val Val Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Lys Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 319
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 319
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catggtcgtc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgtcgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 320
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 320
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ser Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 321
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 321
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc tgtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaaccgtat tgaaagattt ggcgtaa 1197
<210> 322
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 322
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Val Leu Lys Asp Leu Ala
385 390 395
<210> 323
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 323
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgtggag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caaaacaagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccg tgtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt ggcgtaa 1197
<210> 324
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 324
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Val Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asn Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Arg Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Ala
385 390 395
<210> 325
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 325
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc ctggggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagacaagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 326
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 326
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Leu Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 327
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 327
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg caaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaaccgtat tgaaagattt gctgtaa 1197
<210> 328
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 328
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Val Leu Lys Asp Leu Leu
385 390 395
<210> 329
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 329
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaggag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctaa gaaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccg tgtagcctct 1020
ttgggtaagg gtcaggcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaaccagct tgaaagattt ggcgtaa 1197
<210> 330
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 330
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Lys Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Arg Val Ala Ser Leu Gly Lys Gly Gln Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Ala
385 390 395
<210> 331
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 331
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc ctggggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caaaacaagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaaccgtat tgaaagattt gctgtaa 1197
<210> 332
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 332
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Leu Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asn Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Val Leu Lys Asp Leu Leu
385 390 395
<210> 333
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 333
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgtggag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagacaagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaaccgtat tgaaagattt ggcgtaa 1197
<210> 334
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 334
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Val Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Val Leu Lys Asp Leu Ala
385 390 395
<210> 335
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 335
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tcgtgctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc tgtagcctct 1020
ttgggtaagg gtgtcgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 336
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 336
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Arg Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 337
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 337
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc ctggggtaca aacacatgag tcgtgctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctaa gaaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagacaagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc tgtagcctct 1020
ttgggtggtg gtcaggcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaaccgtat tgaaagattt gctgtaa 1197
<210> 338
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 338
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Leu Gly Tyr Lys His Met Ser Arg Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Lys Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Gly Gly Gln Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Val Leu Lys Asp Leu Leu
385 390 395
<210> 339
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 339
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaggag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgag tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tcgtgctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg caaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 340
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 340
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Glu Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Arg Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 341
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 341
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc ctggggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaaccgtat tgaaagattt gctgtaa 1197
<210> 342
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 342
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Leu Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Val Leu Lys Asp Leu Leu
385 390 395
<210> 343
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 343
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaggag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctaa gaaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 344
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 344
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Lys Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 345
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 345
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgag tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tcgtgctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg caaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaaccgtat tgaaagattt gctgtaa 1197
<210> 346
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 346
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Glu Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Arg Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Val Leu Lys Asp Leu Leu
385 390 395
<210> 347
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 347
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgtggag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc ctggggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 348
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 348
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Val Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Leu Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 349
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 349
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc ctggggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc tgtagcctct 1020
ttgggtaagg gtcaggcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaaccgtat tgaaagattt ggcgtaa 1197
<210> 350
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 350
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Leu Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Gln Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Val Leu Lys Asp Leu Ala
385 390 395
<210> 351
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 351
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgtggag 120
atggctgaac ggatggtaga tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 352
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 352
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Val Glu Met Ala Glu Arg Met Val Asp Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 353
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 353
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tcgtgctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaaccgtat tgaaagattt gctgtaa 1197
<210> 354
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 354
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Arg Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Val Leu Lys Asp Leu Leu
385 390 395
<210> 355
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 355
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctagcgatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctacagcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagaagct acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 356
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 356
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Ala Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Ser Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 357
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 357
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt aatataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg cagacctctt tgaaagattt gttataa 1197
<210> 358
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 358
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Asn Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 359
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 359
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
ataaatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg cagacctctt tgaaagattt gttataa 1197
<210> 360
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 360
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asn Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 361
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 361
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttat cgtgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
ataaatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 362
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 362
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Ser Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asn Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 363
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 363
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagaaact acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 364
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 364
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Asn Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 365
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 365
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgcatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctacagcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagaaact acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 366
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 366
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Ser Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Asn Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 367
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 367
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctacagcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt aatataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagaagct acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa 1197
<210> 368
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 368
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Ser Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Asn Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 369
<211> 1197
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 369
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctacagcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagaagct acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgaa caacacaatg cagacctctt tgaaagattt gttataa 1197
<210> 370
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 370
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Ser Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Asn Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 371
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 371
ctggacaatg gattggcaag gacgcctacc atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc tcatggtctc agaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aattatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaaaag tataaagagt atcttggact ggacatcttt taaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggaa tcagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcttagc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatcgc agttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt 1194
<210> 372
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 372
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val
305 310 315 320
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu
385 390 395
<210> 373
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 373
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagacgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 374
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 374
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 375
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 375
ctggacaatg gattggcaag gacgcctaca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc tcatggtctc agaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aattatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaaaag tataaagagt atcttggact ggacatctag gaaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggga ccagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcgatgc ctgggctgta 960
gctattataa accggcagga gattggtgga cctcgctctt ataccatccc cgttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga agctacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt 1194
<210> 376
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 376
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu
385 390 395
<210> 377
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 377
ctggacaatg gattggcaag gacgcctaca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc tcatggtctc agaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aattatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaagag tataaagagt atcttggact ggacatcttt taaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggaa tcagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga gccatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcttagc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatcgc cgttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt 1194
<210> 378
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 378
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val
305 310 315 320
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu
385 390 395
<210> 379
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 379
ctggacaatg gattggcaag gacgcctaca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc tgatggtctc tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aattatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaaaag tataaagagt atcttggact ggacatcttt taaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggaa tcagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga gccatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcgatgc ctgggctgta 960
gctattataa accggcagga gattggtgga cctcgctctt ataccatcgc cgttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt 1194
<210> 380
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 380
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu
385 390 395
<210> 381
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 381
ctggacaatg gattggcaag gacgcctaca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc tgatggtctc tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aattatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaaaag tataaagagt atcttggact ggacatcttt taaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggaa tcagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga gccatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agaaagggag acaactttga agtgtgggaa cgacctctct caggcttagc ctgggctgta 960
gctattataa accggcagga gattggtgga cctcgctctt ataccatcgc agttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt 1194
<210> 382
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 382
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu
385 390 395
<210> 383
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 383
ctggacaatg gattggcaag gacgcctaca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc tcatggtctc agaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aattatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctgggccag tataaagagt atcttggact ggacatctag gaaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggaa tcagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga gccatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcttagc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatcgc agttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagacctcat taaaagactt actt 1194
<210> 384
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 384
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val
305 310 315 320
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 385
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 385
ctggacaatg gattggcaag gacgcctaca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc ggatggtctc tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aattatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctgggccag tataaagagt atcttggact ggacatcttt caaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggaa ccagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcttagc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatccc cgttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt 1194
<210> 386
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 386
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Arg Met Val Ser Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val
305 310 315 320
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu
385 390 395
<210> 387
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 387
ctggacaatg gattggcaag gacgcctaca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc ggatggtctc tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aattatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaaaag tataaagagt atcttggact ggacatcttt taaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggga ccagcaagta actcagatgg ccctctgggc tatcatggct 780
ggtcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agaaagggag acaactttga agtgtgggaa cgacctctct caggcgatgc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatcgc agttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt 1194
<210> 388
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 388
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Arg Met Val Ser Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu
385 390 395
<210> 389
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 389
ctggacaatg gattggcaag gacgcctaca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc ggatggtctc tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aattatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaaaag tataaagagt atcttggact ggacatcttt caaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggga ccagcaagta actcagatgg ccctctgggc tatcatggct 780
ggtcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agaaagggag acaactttga agtgtgggaa cgacctctct caggcgatgc ctgggctgta 960
gctattataa accggcagga gattggtgga cctcgctctt ataccatcgc cgttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt 1194
<210> 390
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 390
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Arg Met Val Ser Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu
385 390 395
<210> 391
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 391
ctggacaatg gattggcaag gacgcctaca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc ggatggtcac tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aattatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctgggccag tataaagagt atcttggact ggacatctag gaaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggga ccagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga gccatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcctcgc ctgggctgta 960
gctattataa accggcagga gattggtgga cctcgctctt ataccatcgc cgttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt 1194
<210> 392
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 392
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu
385 390 395
<210> 393
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 393
ctggacaatg gattggcaag gacgcctacc atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc ggatggtcac tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aattatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaaaag tataaagagt atcttggact ggacatcttt taaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggga ccagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcctcgc ctgggctgta 960
gctattataa accggcagga gattggtgga cctcgctctt ataccatcgc agttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga agctacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt 1194
<210> 394
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 394
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu
385 390 395
<210> 395
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 395
ctggacaatg gattggcaag gacgcctaca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc ggatggtcac tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aattatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaaaag tataaagagt atcttggact ggacatcttt caaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggaa ccagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agaaagggag acaactttga agtgtgggaa cgacctctct caggcgatgc ctgggctgta 960
gctattataa accggcagga gattggtgga cctcgctctt ataccatcgc agttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt 1194
<210> 396
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 396
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu
385 390 395
<210> 397
<211> 1251
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 397
atggaatgga gctgggtctt tctcttcttc ctgtcagtaa cgactggtgt ccactccctg 60
gacaatggat tggcaaggac gcctacaatg ggctggctgc actgggagcg cttcatgtgc 120
aaccttgact gccaggaaga gccagattcc tgcatcagtg agaagctctt catggagatg 180
gcagagcgga tggtcactga aggctggaag gatgcaggtt atgagtacct ctgcattgat 240
gactgttgga tggctcccca aagagattca gaaggcagac ttcaggcaga ccctcagcgc 300
tttcctcatg ggattcgcca gctagctaat catgttcaca gcaaaggact gaagctaggg 360
atttatgcag atgttggaaa taaaacctgc gcaggcttcc ctgggagttt tggatactac 420
gacattgatg cccagacctt tgctgactgg ggagtagatc tgctaaaatt tgatggttgt 480
tactgtgaca gtttggaaaa tttggcagat ggttataagc acatgtcctt ggccctgaat 540
aggactggca gaagcattgt gtactcctgt gagtggcctc tttatatgtg gccctttcaa 600
aagcccaatt atacagaaat ccgacagtac tgcaatcact ggcgaaattt tgctgacatt 660
gatgattcct gggccagtat aaagagtatc ttggactgga catctaggaa ccaggagaga 720
attgttgatg ttgctggacc agggggttgg aatgacccag atatgttagt gattggcaac 780
tttggcctca gctggaacca gcaagtaact cagatggccc tctgggctat catggctgct 840
cctttattca tgtctaatga cctccgacac atcagccctc aagccaaagc tctccttcag 900
gataaggacg taattgccat caatcaggac cccttgggca agcaagggta ccagcttaga 960
cagggagaca actttgaagt gtgggaacga cctctctcag gcgatgcctg ggctgtagct 1020
attataaacc ggcaggagat tggtggacct cgctcttata ccatcgcagt tgcttccctg 1080
ggtaaaggag tggcctgtaa tcctgcctgc ttcatcacac agctcctccc tgtgaaaagg 1140
aagctagggt tctatgaatg gacttcaagg ttaagaagtc acataaatcc cacaggcact 1200
gttttgcttc agctagaaaa tacaatgcag atgtcattaa aagacttact t 1251
<210> 398
<211> 417
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 398
Met Glu Trp Ser Trp Val Phe Leu Phe Phe Leu Ser Val Thr Thr Gly
1 5 10 15
Val His Ser Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp
20 25 30
Leu His Trp Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro
35 40 45
Asp Ser Cys Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Arg Met
50 55 60
Val Thr Glu Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp
65 70 75 80
Asp Cys Trp Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala
85 90 95
Asp Pro Gln Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val
100 105 110
His Ser Lys Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys
115 120 125
Thr Cys Ala Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala
130 135 140
Gln Thr Phe Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys
145 150 155 160
Tyr Cys Asp Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser
165 170 175
Leu Ala Leu Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp
180 185 190
Pro Leu Tyr Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg
195 200 205
Gln Tyr Cys Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp
210 215 220
Ala Ser Ile Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg
225 230 235 240
Ile Val Asp Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu
245 250 255
Val Ile Gly Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met
260 265 270
Ala Leu Trp Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu
275 280 285
Arg His Ile Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val
290 295 300
Ile Ala Ile Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg
305 310 315 320
Gln Gly Asp Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala
325 330 335
Trp Ala Val Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser
340 345 350
Tyr Thr Ile Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro
355 360 365
Ala Cys Phe Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe
370 375 380
Tyr Glu Trp Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr
385 390 395 400
Val Leu Leu Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu
405 410 415
Leu
<210> 399
<211> 1251
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 399
atggaatgga gctgggtctt tctcttcttc ctgtcagtaa cgactggtgt ccactccctg 60
gacaatggat tggcaaggac gcctacaatg ggctggctgc actgggagcg cttcatgtgc 120
aaccttgact gccaggaaga gccagattcc tgcatcagtg agaagctctt catggagatg 180
gcagagcgga tggtcactga aggctggaag gatgcaggtt atgagtacct ctgcattgat 240
gactgttgga tggctcccca aagagattca gaaggcagac ttcaggcaga ccctcagcgc 300
tttcctcatg ggattcgcca gctagctaat catgttcaca gcaaaggact gaagctaggg 360
atttatgcag atgttggaaa taaaacctgc gcaggcttcc ctgggagttt tggatactac 420
gacattgatg cccagacctt tgctgactgg ggagtagatc tgctaaaatt tgatggttgt 480
tactgtgaca gtttggaaaa tttggcagat ggttataagc acatgtcctt ggccctgaat 540
aggactggca gaagcattgt gtactcctgt gagtggcctc tttatatgtg gccctttcaa 600
aagcccaatt atacagaaat ccgacagtac tgcaatcact ggcgaaattt tgctgacatt 660
gatgattcct ggaaaagtat aaagagtatc ttggactgga catcttttaa ccaggagaga 720
attgttgatg ttgctggacc agggggttgg aatgacccag atatgttagt gattggcaac 780
tttggcctca gctgggacca gcaagtaact cagatggccc tctgggctat catggctggt 840
cctttattca tgtctaatga cctccgagcc atcagccctc aagccaaagc tctccttcag 900
gataaggacg taattgccat caatcaggac cccttgggca agcaagggta ccagcttaga 960
cagggagaca actttgaagt gtgggaacga cctctctcag gcgatgcctg ggctgtagct 1020
atgataaacc ggcaggagat tggtggacct cgctcttata ccatccccgt tgcttccctg 1080
ggtaaaggag tggcctgtaa tcctgcctgc ttcatcacac agctcctccc tgtgaaaagg 1140
aagctagggt tctatgaagc tacttcaagg ttaagaagtc acataaatcc cacaggcact 1200
gttttgcttc agctagaaaa tacaatgcag atgtcattaa aagacttact t 1251
<210> 400
<211> 417
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 400
Met Glu Trp Ser Trp Val Phe Leu Phe Phe Leu Ser Val Thr Thr Gly
1 5 10 15
Val His Ser Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp
20 25 30
Leu His Trp Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro
35 40 45
Asp Ser Cys Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Arg Met
50 55 60
Val Thr Glu Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp
65 70 75 80
Asp Cys Trp Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala
85 90 95
Asp Pro Gln Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val
100 105 110
His Ser Lys Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys
115 120 125
Thr Cys Ala Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala
130 135 140
Gln Thr Phe Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys
145 150 155 160
Tyr Cys Asp Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser
165 170 175
Leu Ala Leu Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp
180 185 190
Pro Leu Tyr Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg
195 200 205
Gln Tyr Cys Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp
210 215 220
Lys Ser Ile Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg
225 230 235 240
Ile Val Asp Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu
245 250 255
Val Ile Gly Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met
260 265 270
Ala Leu Trp Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu
275 280 285
Arg Ala Ile Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val
290 295 300
Ile Ala Ile Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg
305 310 315 320
Gln Gly Asp Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala
325 330 335
Trp Ala Val Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser
340 345 350
Tyr Thr Ile Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro
355 360 365
Ala Cys Phe Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe
370 375 380
Tyr Glu Ala Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr
385 390 395 400
Val Leu Leu Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu
405 410 415
Leu
<210> 401
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 401
ctggacaatg gattggcaag gacgcctaca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc ggatggtctc tgaaggctgg aaggatgcag gttatgagta cctatgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aatcatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctgggccag tataaagagt atcttggact ggacatcttt caaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggga ccagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcttagc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatcgc cgttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga agctacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt 1194
<210> 402
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 402
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Arg Met Val Ser Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val
305 310 315 320
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu
385 390 395
<210> 403
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 403
ctggacaatg gattggcaag gacgcctaca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc tgatggtctc tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagatagct aattatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaagag tataaagagt atcttggact ggacatctag gaaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggga ccagcaagta actcagatgg ccctctgggc tatcatggct 780
ggtcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agaaagggag acaactttga agtgtgggaa cgacctctct caggcgatgc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatcgc agttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt 1194
<210> 404
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 404
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Ile Ala Asn Tyr Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu
385 390 395
<210> 405
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 405
ctggacaatg gattggcaag gacgcctaca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcgaggag 120
atggcagagc ggatggtctc tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aatcatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaagag tataaagagt atcttggact ggacatctag gaaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggaa tcagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcctcgc ctgggctgta 960
gctattataa accggcagga gattggtgga cctcgctctt ataccatcgc agttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt 1194
<210> 406
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 406
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Ser Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu
385 390 395
<210> 407
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 407
ctggacaatg gattggcaag gacgcctacc atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcgaggag 120
atggcagagc ggatggtctc tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aatcatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaaaag tataaagagt atcttggact ggacatcttt taaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggga ccagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga gccatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agaaagggag acaactttga agtgtgggaa cgacctctct caggcttagc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatcgc agttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt 1194
<210> 408
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 408
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Ser Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val
305 310 315 320
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu
385 390 395
<210> 409
<211> 1251
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 409
atggaatgga gctgggtctt tctcttcttc ctgtcagtaa cgactggtgt ccactccctg 60
gacaatggat tggcaaggac gcctacaatg ggctggctgc actgggagcg cttcatgtgc 120
aaccttgact gccaggaaga gccagattcc tgcatcagtg agaagctctt cgaggagatg 180
gcagagcgga tggtctctga aggctggaag gatgcaggtt atgagtacct ctgcattgat 240
gactgttgga tggctcccca aagagattca gaaggcagac ttcaggcaga ccctcagcgc 300
tttcctcatg ggattcgcca gctagctaat catgttcaca gcaaaggact gaagctaggg 360
atttatgcag atgttggaaa taaaacctgc gcaggcttcc ctgggagttt tggatactac 420
gacattgatg cccagacctt tgctgactgg ggagtagatc tgctaaaatt tgatggttgt 480
tactgtgaca gtttggaaaa tttggcagat ggttataagc acatgtcctt ggccctgaat 540
atgactggca gaagcattgt gtactcctgt gagtggcctc tttatatgtg gccctttcaa 600
aagcccaatt atacagaaat ccgacagtac tgcaatcact ggcgaaattt tgctgacatt 660
gatgattcct ggaaaagtat aaagagtatc ttggactgga catctttcaa ccaggagaga 720
attgttgatg ttgctggacc agggggttgg aatgacccag atatgttagt gattggcaac 780
tttggcctca gctgggacca gcaagtaact cagatggccc tctgggctat catggctgct 840
cctttattca tgtctaatga cctccgacac atcagccctc aagccaaagc tctccttcag 900
gataaggacg taattgccat caatcaggac cccttgggca agcaagggta ccagcttaga 960
aagggagaca actttgaagt gtgggaacga cctctctcag gcgatgcctg ggctgtagct 1020
attataaacc ggcaggagat tggtggacct cgctcttata ccatcgccgt tgcttccctg 1080
ggtaaaggag tggcctgtaa tcctgcctgc ttcatcacac agctcctccc tgtgaaaagg 1140
aagctagggt tctatgaatg gacttcaagg ttaagaagtc acataaatcc cacaggcact 1200
gttttgcttc agctagaaaa tacaatgcag atgtcattaa aagacttact t 1251
<210> 410
<211> 417
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 410
Met Glu Trp Ser Trp Val Phe Leu Phe Phe Leu Ser Val Thr Thr Gly
1 5 10 15
Val His Ser Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp
20 25 30
Leu His Trp Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro
35 40 45
Asp Ser Cys Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met
50 55 60
Val Ser Glu Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp
65 70 75 80
Asp Cys Trp Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala
85 90 95
Asp Pro Gln Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val
100 105 110
His Ser Lys Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys
115 120 125
Thr Cys Ala Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala
130 135 140
Gln Thr Phe Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys
145 150 155 160
Tyr Cys Asp Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser
165 170 175
Leu Ala Leu Asn Met Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp
180 185 190
Pro Leu Tyr Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg
195 200 205
Gln Tyr Cys Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp
210 215 220
Lys Ser Ile Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg
225 230 235 240
Ile Val Asp Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu
245 250 255
Val Ile Gly Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met
260 265 270
Ala Leu Trp Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu
275 280 285
Arg His Ile Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val
290 295 300
Ile Ala Ile Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg
305 310 315 320
Lys Gly Asp Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala
325 330 335
Trp Ala Val Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser
340 345 350
Tyr Thr Ile Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro
355 360 365
Ala Cys Phe Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe
370 375 380
Tyr Glu Trp Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr
385 390 395 400
Val Leu Leu Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu
405 410 415
Leu
<210> 411
<211> 1251
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 411
atggaatgga gctgggtctt tctcttcttc ctgtcagtaa cgactggtgt ccactccctg 60
gacaatggat tggcaaggac gcctacaatg ggctggctgc actgggagcg cttcatgtgc 120
aaccttgact gccaggaaga gccagattcc tgcatcagtg agaagctctt cgaggagatg 180
gcagagctga tggtctctga aggctggaag gatgcaggtt atgagtacct ctgcattgat 240
gactgttgga tggctcccca aagagattca gaaggcagac ttcaggcaga ccctcagcgc 300
tttcctcatg ggattcgcca gctagctaat tatgttcaca gcaaaggact gaagctaggg 360
atttatgcag atgttggaaa taaaacctgc gcaggcttcc ctgggagttt tggatactac 420
gacattgatg cccagacctt tgctgactgg ggagtagatc tgctaaaatt tgatggttgt 480
tactgtgaca gtttggaaaa tttggcagat ggttataagc acatgtcctt ggccctgaat 540
aggactggca gaagcattgt gtactcctgt gagtggcctc tttatatgtg gccctttcaa 600
aagcccaatt atacagaaat ccgacagtac tgcaatcact ggcgaaattt tgctgacatt 660
gatgattcct ggaagagtat aaagagtatc ttggactgga catcttttaa ccaggagaga 720
attgttgatg ttgctggacc agggggttgg aatgacccag atatgttagt gattggcaac 780
tttggcctca gctggaacca gcaagtaact cagatggccc tctgggctat catggctgct 840
cctttattca tgtctaatga cctccgacac atcagccctc aagccaaagc tctccttcag 900
gataaggacg taattgccat caatcaggac cccttgggca agcaagggta ccagcttaga 960
cagggagaca actttgaagt gtgggaacga cctctctcag gcctcgcctg ggctgtagct 1020
attataaacc ggcaggagat tggtggacct cgctcttata ccatcgcagt tgcttccctg 1080
ggtaaaggag tggcctgtaa tcctgcctgc ttcatcacac agctcctccc tgtgaaaagg 1140
aagctagggt tctatgaatg gacttcaagg ttaagaagtc acataaatcc cacaggcact 1200
gttttgcttc agctagaaaa tacaatgcag atgtcattaa aagacttact t 1251
<210> 412
<211> 417
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 412
Met Glu Trp Ser Trp Val Phe Leu Phe Phe Leu Ser Val Thr Thr Gly
1 5 10 15
Val His Ser Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp
20 25 30
Leu His Trp Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro
35 40 45
Asp Ser Cys Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met
50 55 60
Val Ser Glu Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp
65 70 75 80
Asp Cys Trp Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala
85 90 95
Asp Pro Gln Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val
100 105 110
His Ser Lys Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys
115 120 125
Thr Cys Ala Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala
130 135 140
Gln Thr Phe Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys
145 150 155 160
Tyr Cys Asp Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser
165 170 175
Leu Ala Leu Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp
180 185 190
Pro Leu Tyr Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg
195 200 205
Gln Tyr Cys Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp
210 215 220
Lys Ser Ile Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg
225 230 235 240
Ile Val Asp Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu
245 250 255
Val Ile Gly Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met
260 265 270
Ala Leu Trp Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu
275 280 285
Arg His Ile Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val
290 295 300
Ile Ala Ile Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg
305 310 315 320
Gln Gly Asp Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala
325 330 335
Trp Ala Val Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser
340 345 350
Tyr Thr Ile Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro
355 360 365
Ala Cys Phe Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe
370 375 380
Tyr Glu Trp Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr
385 390 395 400
Val Leu Leu Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu
405 410 415
Leu
<210> 413
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 413
ctggacaatg gattggcaag gacgcctaca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcgaggag 120
atggcagagc tgatggtctc tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aattatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaaaag tataaagagt atcttggact ggacatcttt taaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggga ccagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga gccatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcttagc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatcgc agttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt 1194
<210> 414
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 414
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Ser Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val
305 310 315 320
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu
385 390 395
<210> 415
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 415
ctggacaatg gattggcaag gacgcctaca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcgaggag 120
atggcagagc tgatggtctc tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aattatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaaaag tataaagagt atcttggact ggacatcttt taaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggga ccagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga gccatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctcttt caggcgatgc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatcgc cgttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt 1194
<210> 416
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 416
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Ser Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu
385 390 395
<210> 417
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 417
ctggacaatg gattggcaag gacgcctaca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcgaggag 120
atggcagagc tgatggtcac tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aattatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaaaag tataaagagt atcttggact ggacatctag gaaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggga ccagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcttagc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatcgc cgttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga agctacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt 1194
<210> 418
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 418
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val
305 310 315 320
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu
385 390 395
<210> 419
<211> 1251
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 419
atggaatgga gctgggtctt tctcttcttc ctgtcagtaa cgactggtgt ccactccctg 60
gacaatggat tggcaaggac gcctacaatg ggctggctgc actgggagcg cttcatgtgc 120
aaccttgact gccaggaaga gccagattcc tgcatcagtg agaagctctt cgaggagatg 180
gcagagctga tggtcactga aggctggaag gatgcaggtt atgagtacct ctgcattgat 240
gactgttgga tggctcccca aagagattca gaaggcagac ttcaggcaga ccctcagcgc 300
tttcctcatg ggattcgcca gctagctaat tatgttcaca gcaaaggact gaagctaggg 360
atttatgcag atgttggaaa taaaacctgc gcaggcttcc ctgggagttt tggatactac 420
gacattgatg cccagacctt tgctgactgg ggagtagatc tgctaaaatt tgatggttgt 480
tactgtgaca gtttggaaaa tttggcagat ggttataagc acatgtcctt ggccctgaat 540
aggactggca gaagcattgt gtactcctgt gagtggcctc tttatatgtg gccctttcaa 600
aagcccaatt atacagaaat ccgacagtac tgcaatcact ggcgaaattt tgctgacatt 660
gatgattcct ggaaaagtat aaagagtatc ttggactgga catctttcaa ccaggagaga 720
attgttgatg ttgctggacc agggggttgg aatgacccag atatgttagt gattggcaac 780
tttggcctca gctgggacca gcaagtaact cagatggccc tctgggctat catggctgct 840
cctttattca tgtctaatga cctccgacac atcagccctc aagccaaagc tctccttcag 900
gataaggacg taattgccat caatcaggac cccttgggca agcaagggta ccagcttaga 960
cagggagaca actttgaagt gtgggaacga cctctctcag gcctcgcctg ggctgtagct 1020
atgataaacc ggcaggagat tggtggacct cgctcttata ccatcgcagt tgcttccctg 1080
ggtaaaggag tggcctgtaa tcctgcctgc ttcatcacac agctcctccc tgtgaaaagg 1140
aagctagggt tctatgaatg gacttcaagg ttaagaagtc acataaatcc cacaggcact 1200
gttttgcttc agctagaaaa tacaatgcag atgtcattaa aagacttact t 1251
<210> 420
<211> 417
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 420
Met Glu Trp Ser Trp Val Phe Leu Phe Phe Leu Ser Val Thr Thr Gly
1 5 10 15
Val His Ser Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp
20 25 30
Leu His Trp Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro
35 40 45
Asp Ser Cys Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met
50 55 60
Val Thr Glu Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp
65 70 75 80
Asp Cys Trp Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala
85 90 95
Asp Pro Gln Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val
100 105 110
His Ser Lys Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys
115 120 125
Thr Cys Ala Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala
130 135 140
Gln Thr Phe Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys
145 150 155 160
Tyr Cys Asp Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser
165 170 175
Leu Ala Leu Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp
180 185 190
Pro Leu Tyr Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg
195 200 205
Gln Tyr Cys Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp
210 215 220
Lys Ser Ile Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg
225 230 235 240
Ile Val Asp Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu
245 250 255
Val Ile Gly Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met
260 265 270
Ala Leu Trp Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu
275 280 285
Arg His Ile Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val
290 295 300
Ile Ala Ile Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg
305 310 315 320
Gln Gly Asp Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala
325 330 335
Trp Ala Val Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser
340 345 350
Tyr Thr Ile Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro
355 360 365
Ala Cys Phe Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe
370 375 380
Tyr Glu Trp Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr
385 390 395 400
Val Leu Leu Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu
405 410 415
Leu
<210> 421
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 421
ctggacaatg gattggcaag gacgcctacc atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcgaggag 120
atggcagagc tgatggtcac tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aatcatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaaaag tataaagagt atcttggact ggacatcttt caaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggga ccagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agaaagggag acaactttga agtgtgggaa cgacctctct caggcgatgc ctgggctgta 960
gctattataa accggcagga gattggtgga cctcgctctt ataccatcgc agttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt 1194
<210> 422
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 422
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu
385 390 395
<210> 423
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 423
ctggacaatg gattggcaag gacgcctaca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcgaggag 120
atggcagagc tgatggtctc tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aatcatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaaaag tataaagagt atcttggact ggacatcttt caaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggaa tcagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcgatgc ctgggctgta 960
gctattataa accggcagga gattggtgga cctcgctctt ataccatcgc agttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt 1194
<210> 424
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 424
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Ser Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu
385 390 395
<210> 425
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 425
ctggacaatg gattggcaag gacgcctacc atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcgaggag 120
atggcagagc tgatggtctc tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aatcatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaaaag tataaagagt atcttggact ggacatcttt taaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggga ccagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agaaagggag acaactttga agtgtgggaa cgacctctct caggcgatgc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatccc cgttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga agctacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt 1194
<210> 426
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 426
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Ser Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu
385 390 395
<210> 427
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 427
ctggacaatg gattggcaag gacgcctaca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc tcatggtctc agaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aattatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaaaag tataaagagt atcttggact ggacatcttt taaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggga ccagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcttagc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatcgc agttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt 1194
<210> 428
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 428
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val
305 310 315 320
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu
385 390 395
<210> 429
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 429
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaagaa 120
atggccgaac tgatggtgac cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattgc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agcaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 430
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 430
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 431
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 431
ctggataatg gattggctag aacacctccc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaagaa 120
atggccgaac tgatggtgtc cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagttt taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc gctatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaagggcg acaactttga ggtctgggaa aggcctttga gtggactcgc gtgggctgtg 960
gccatgatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agcaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagatgtcac tgaaggacct cctg 1194
<210> 432
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 432
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Ser Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val
305 310 315 320
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu
385 390 395
<210> 433
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 433
ctggacaatg gattggcaag gacgcctaca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc tcatggtctc agaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aattatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaaaag tataaagagt atcttggact ggacatcttt taaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggga ccagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga gccatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcttagc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatcgc cgttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt 1194
<210> 434
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 434
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val
305 310 315 320
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu
385 390 395
<210> 435
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 435
ctggacaatg gattggcaag gacgcctaca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc tcatggtctc agaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aattatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaaaag tataaagagt atcttggact ggacatcttt caaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggga ccagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agaaagggag acaactttga agtgtgggaa cgacctctct caggcctcgc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatcgc cgttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt 1194
<210> 436
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 436
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val
305 310 315 320
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu
385 390 395
<210> 437
<211> 1251
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 437
atggaatgga gctgggtctt tctcttcttc ctgtcagtaa cgactggtgt ccactccctg 60
gacaatggat tggcaaggac gcctacaatg ggctggctgc actgggagcg cttcatgtgc 120
aaccttgact gccaggaaga gccagattcc tgcatcagtg agaagctctt catggagatg 180
gcagagctga tggtctctga aggctggaag gatgcaggtt atgagtacct ctgcattgat 240
gactgttgga tggctcccca aagagattca gaaggcagac ttcaggcaga ccctcagcgc 300
tttcctcatg ggattcgcca gctagctaat tatgttcaca gcaaaggact gaagctaggg 360
atttatgcag atgttggaaa taaaacctgc gcaggcttcc ctgggagttt tggatactac 420
gacattgatg cccagacctt tgctgactgg ggagtagatc tgctaaaatt tgatggttgt 480
tactgtgaca gtttggaaaa tttggcagat ggttataagc acatgtcctt ggccctgaat 540
aggactggca gaagcattgt gtactcctgt gagtggcctc tttatatgtg gccctttcaa 600
aagcccaatt atacagaaat ccgacagtac tgcaatcact ggcgaaattt tgctgacatt 660
gatgattcct ggaaaagtat aaagagtatc ttggactgga catctttcaa ccaggagaga 720
attgttgatg ttgctggacc agggggttgg aatgacccag atatgttagt gattggcaac 780
tttggcctca gctggaacca gcaagtaact cagatggccc tctgggctat catggctgct 840
cctttattca tgtctaatga cctccgacac atcagccctc aagccaaagc tctccttcag 900
gataaggacg taattgccat caatcaggac cccttgggca agcaagggta ccagcttaga 960
aagggagaca actttgaagt gtgggaacga cctctctcag gcttagcctg ggctgtagct 1020
attataaacc ggcaggagat tggtggacct cgctcttata ccatcgcagt tgcttccctg 1080
ggtaaaggag tggcctgtaa tcctgcctgc ttcatcacac agctcctccc tgtgaaaagg 1140
aagctagggt tctatgaagc tacttcaagg ttaagaagtc acataaatcc cacaggcact 1200
gttttgcttc agctagaaaa tacaatgcag atgtcattaa aagacttact t 1251
<210> 438
<211> 417
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 438
Met Glu Trp Ser Trp Val Phe Leu Phe Phe Leu Ser Val Thr Thr Gly
1 5 10 15
Val His Ser Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp
20 25 30
Leu His Trp Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro
35 40 45
Asp Ser Cys Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met
50 55 60
Val Ser Glu Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp
65 70 75 80
Asp Cys Trp Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala
85 90 95
Asp Pro Gln Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val
100 105 110
His Ser Lys Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys
115 120 125
Thr Cys Ala Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala
130 135 140
Gln Thr Phe Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys
145 150 155 160
Tyr Cys Asp Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser
165 170 175
Leu Ala Leu Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp
180 185 190
Pro Leu Tyr Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg
195 200 205
Gln Tyr Cys Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp
210 215 220
Lys Ser Ile Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg
225 230 235 240
Ile Val Asp Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu
245 250 255
Val Ile Gly Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met
260 265 270
Ala Leu Trp Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu
275 280 285
Arg His Ile Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val
290 295 300
Ile Ala Ile Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg
305 310 315 320
Lys Gly Asp Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala
325 330 335
Trp Ala Val Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser
340 345 350
Tyr Thr Ile Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro
355 360 365
Ala Cys Phe Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe
370 375 380
Tyr Glu Ala Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr
385 390 395 400
Val Leu Leu Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu
405 410 415
Leu
<210> 439
<211> 1251
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 439
atggaatgga gctgggtctt tctcttcttc ctgtcagtaa cgactggtgt ccactccctg 60
gacaatggat tggcaaggac gcctacaatg ggctggctgc actgggagcg cttcatgtgc 120
aaccttgact gccaggaaga gccagattcc tgcatcagtg agaagctctt catggagatg 180
gcagagctga tggtcactga aggctggaag gatgcaggtt atgagtacct ctgcattgat 240
gactgttgga tggctcccca aagagattca gaaggcagac ttcaggcaga ccctcagcgc 300
tttcctcatg ggattcgcca gctagctaat tatgttcaca gcaaaggact gaagctaggg 360
atttatgcag atgttggaaa taaaacctgc gcaggcttcc ctgggagttt tggatactac 420
gacattgatg cccagacctt tgctgactgg ggagtagatc tgctaaaatt tgatggttgt 480
tactgtgaca gtttggaaaa tttggcagat ggttataagc acatgtcctt ggccctgaat 540
aggactggca gaagcattgt gtactcctgt gagtggcctc tttatatgtg gccctttcaa 600
aagcccaatt atacagaaat ccgacagtac tgcaatcact ggcgaaattt tgctgacatt 660
gatgattcct ggaaaagtat aaagagtatc ttggactgga catctaggaa ccaggagaga 720
attgttgatg ttgctggacc agggggttgg aatgacccag atatgttagt gattggcaac 780
tttggcctca gctggaatca gcaagtaact cagatggccc tctgggctat catggctgct 840
cctttattca tgtctaatga cctccgacac atcagccctc aagccaaagc tctccttcag 900
gataaggacg taattgccat caatcaggac cccttgggca agcaagggta ccagcttaga 960
aagggagaca actttgaagt gtgggaacga cctctctcag gcttagcctg ggctgtagct 1020
atgataaacc ggcaggagat tggtggacct cgctcttata ccatcgcagt tgcttccctg 1080
ggtaaaggag tggcctgtaa tcctgcctgc ttcatcacac agctcctccc tgtgaaaagg 1140
aagctagggt tctatgaatg gacttcaagg ttaagaagtc acataaatcc cacaggcact 1200
gttttgcttc agctagaaaa tacaatgcag atgtcattaa aagacttact t 1251
<210> 440
<211> 417
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 440
Met Glu Trp Ser Trp Val Phe Leu Phe Phe Leu Ser Val Thr Thr Gly
1 5 10 15
Val His Ser Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp
20 25 30
Leu His Trp Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro
35 40 45
Asp Ser Cys Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met
50 55 60
Val Thr Glu Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp
65 70 75 80
Asp Cys Trp Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala
85 90 95
Asp Pro Gln Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val
100 105 110
His Ser Lys Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys
115 120 125
Thr Cys Ala Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala
130 135 140
Gln Thr Phe Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys
145 150 155 160
Tyr Cys Asp Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser
165 170 175
Leu Ala Leu Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp
180 185 190
Pro Leu Tyr Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg
195 200 205
Gln Tyr Cys Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp
210 215 220
Lys Ser Ile Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg
225 230 235 240
Ile Val Asp Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu
245 250 255
Val Ile Gly Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met
260 265 270
Ala Leu Trp Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu
275 280 285
Arg His Ile Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val
290 295 300
Ile Ala Ile Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg
305 310 315 320
Lys Gly Asp Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala
325 330 335
Trp Ala Val Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser
340 345 350
Tyr Thr Ile Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro
355 360 365
Ala Cys Phe Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe
370 375 380
Tyr Glu Trp Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr
385 390 395 400
Val Leu Leu Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu
405 410 415
Leu
<210> 441
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 441
ctggacaatg gattggcaag gacgcctaca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc tgatggtcac tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aattatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaaaag tataaagagt atcttggact ggacatcttt caaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggga ccagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcttagc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatcgc agttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt 1194
<210> 442
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 442
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val
305 310 315 320
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu
385 390 395
<210> 443
<211> 1251
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 443
atggaatgga gctgggtctt tctcttcttc ctgtcagtaa cgactggtgt ccactccctg 60
gacaatggat tggcaaggac gcctacaatg ggctggctgc actgggagcg cttcatgtgc 120
aaccttgact gccaggaaga gccagattcc tgcatcagtg agaagctctt catggagatg 180
gcagagctga tggtcactga aggctggaag gatgcaggtt atgagtacct ctgcattgat 240
gactgttgga tggctcccca aagagattca gaaggcagac ttcaggcaga ccctcagcgc 300
tttcctcatg ggattcgcca gctagctaat tatgttcaca gcaaaggact gaagctaggg 360
atttatgcag atgttggaaa taaaacctgc gcaggcttcc ctgggagttt tggatactac 420
gacattgatg cccagacctt tgctgactgg ggagtagatc tgctaaaatt tgatggttgt 480
tactgtgaca gtttggaaaa tttggcagat ggttataagc acatgtcctt ggccctgaat 540
aggactggca gaagcattgt gtactcctgt gagtggcctc tttatatgtg gccctttcaa 600
aagcccaatt atacagaaat ccgacagtac tgcaatcact ggcgaaattt tgctgacatt 660
gatgattcct ggaaaagtat aaagagtatc ttggactgga catctttcaa ccaggagaga 720
attgttgatg ttgctggacc agggggttgg aatgacccag atatgttagt gattggcaac 780
tttggcctca gctgggacca gcaagtaact cagatggccc tctgggctat catggctgct 840
cctttattca tgtctaatga cctccgagcc atcagccctc aagccaaagc tctccttcag 900
gataaggacg taattgccat caatcaggac cccttgggca agcaagggta ccagcttaga 960
cagggagaca actttgaagt gtgggaacga cctctctcag gcttagcctg ggctgtagct 1020
atgataaacc ggcaggagat tggtggacct cgctcttata ccatcgcagt tgcttccctg 1080
ggtaaaggag tggcctgtaa tcctgcctgc ttcatcacac agctcctccc tgtgaaaagg 1140
aagctagggt tctatgaatg gacttcaagg ttaagaagtc acataaatcc cacaggcact 1200
gttttgcttc agctagaaaa tacaatgcag atgtcattaa aagacttact t 1251
<210> 444
<211> 417
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 444
Met Glu Trp Ser Trp Val Phe Leu Phe Phe Leu Ser Val Thr Thr Gly
1 5 10 15
Val His Ser Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp
20 25 30
Leu His Trp Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro
35 40 45
Asp Ser Cys Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met
50 55 60
Val Thr Glu Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp
65 70 75 80
Asp Cys Trp Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala
85 90 95
Asp Pro Gln Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val
100 105 110
His Ser Lys Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys
115 120 125
Thr Cys Ala Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala
130 135 140
Gln Thr Phe Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys
145 150 155 160
Tyr Cys Asp Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser
165 170 175
Leu Ala Leu Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp
180 185 190
Pro Leu Tyr Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg
195 200 205
Gln Tyr Cys Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp
210 215 220
Lys Ser Ile Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg
225 230 235 240
Ile Val Asp Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu
245 250 255
Val Ile Gly Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met
260 265 270
Ala Leu Trp Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu
275 280 285
Arg Ala Ile Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val
290 295 300
Ile Ala Ile Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg
305 310 315 320
Gln Gly Asp Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala
325 330 335
Trp Ala Val Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser
340 345 350
Tyr Thr Ile Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro
355 360 365
Ala Cys Phe Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe
370 375 380
Tyr Glu Trp Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr
385 390 395 400
Val Leu Leu Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu
405 410 415
Leu
<210> 445
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 445
ctggacaatg gattggcaag gacgcctacc atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc tgatggtcac tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aatcatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaaaag tataaagagt atcttggact ggacatcttt caaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggga ccagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga gccatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcttagc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatcgc cgttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt 1194
<210> 446
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 446
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val
305 310 315 320
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu
385 390 395
<210> 447
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 447
ctggacaatg gattggcaag gacgcctcca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc tcatggtctc agaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aattatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaaaag tataaagagt atcttggact ggacatcttt taaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggaa ccagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcttagc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatcgc agttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt 1194
<210> 448
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 448
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val
305 310 315 320
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu
385 390 395
<210> 449
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 449
ctggacaatg gattggcaag gacgcctcca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc tcatggtctc agaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aattatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaaaag tataaagagt atcttggact ggacatcttt taaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggaa ccagcaagta actcagatgg ccctctgggc tatcatggct 780
ggtcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcttagc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatcgc agttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt 1194
<210> 450
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 450
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val
305 310 315 320
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu
385 390 395
<210> 451
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 451
ctggacaatg gattggcaag gacgcctcca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc tcatggtctc agaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aattatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaagag tataaagagt atcttggact ggacatcttt taaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggaa tcagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcttagc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatccc cgttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagacctcat taaaagactt actt 1194
<210> 452
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 452
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val
305 310 315 320
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 453
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 453
ctggacaatg gattggcaag gacgcctcca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc tcatggtctc agaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aattatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaaaag tataaagagt atcttggact ggacatcttt taaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggaa tcagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga gccatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agaaagggag acaactttga agtgtgggaa cgacctctct caggcttagc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatcgc agttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt 1194
<210> 454
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 454
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val
305 310 315 320
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu
385 390 395
<210> 455
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 455
ctggacaatg gattggcaag gacgcctcca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc tgatggtctc tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aattatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctgggccag tataaagagt atcttggact ggacatcttt caaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggaa ccagcaagta actcagatgg ccctctgggc tatcatggct 780
ggtcctttat tcatgtctaa tgacctccga gccatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcgatgc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatcgc cgttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt 1194
<210> 456
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 456
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu
385 390 395
<210> 457
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 457
ctggacaatg gattggcaag gacgcctcca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc tcatggtctc agaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aattatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctgggccag tataaagagt atcttggact ggacatctag gaaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggaa ccagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga gccatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcctcgc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatcgc agttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt 1194
<210> 458
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 458
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val
305 310 315 320
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu
385 390 395
<210> 459
<211> 1251
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 459
atggaatgga gctgggtctt tctcttcttc ctgtcagtaa cgactggtgt ccactccctg 60
gacaatggat tggcaaggac gcctccaatg ggctggctgc actgggagcg cttcatgtgc 120
aaccttgact gccaggaaga gccagattcc tgcatcagtg agaagctctt catggagatg 180
gcagagctca tggtctcaga aggctggaag gatgcaggtt atgagtacct ctgcattgat 240
gactgttgga tggctcccca aagagattca gaaggcagac ttcaggcaga ccctcagcgc 300
tttcctcatg ggattcgcca gctagctaat tatgttcaca gcaaaggact gaagctaggg 360
atttatgcag atgttggaaa taaaacctgc gcaggcttcc ctgggagttt tggatactac 420
gacattgatg cccagacctt tgctgactgg ggagtagatc tgctaaaatt tgatggttgt 480
tactgtgaca gtttggaaaa tttggcagat ggttataagc acatgtcctt ggccctgaat 540
aggactggca gaagcattgt gtactcctgt gagtggcctc tttatatgtg gccctttcaa 600
aagcccaatt atacagaaat ccgacagtac tgcaatcact ggcgaaattt tgctgacatt 660
gatgattcct gggccagtat aaagagtatc ttggactgga catcttttaa ccaggagaga 720
attgttgatg ttgctggacc agggggttgg aatgacccag atatgttagt gattggcaac 780
tttggcctca gctgggacca gcaagtaact cagatggccc tctgggctat catggctgct 840
cctttattca tgtctaatga cctccgacac atcagccctc aagccaaagc tctccttcag 900
gataaggacg taattgccat caatcaggac cccttgggca agcaagggta ccagcttaga 960
cagggagaca actttgaagt gtgggaacga cctctctcag gcttagcctg ggctgtagct 1020
atgataaacc ggcaggagat tggtggacct cgctcttata ccatcgcagt tgcttccctg 1080
ggtaaaggag tggcctgtaa tcctgcctgc ttcatcacac agctcctccc tgtgaaaagg 1140
aagctagggt tctatgaatg gacttcaagg ttaagaagtc acataaatcc cacaggcact 1200
gttttgcttc agctagaaaa tacaatgcag atgtcattaa aagacttact t 1251
<210> 460
<211> 417
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 460
Met Glu Trp Ser Trp Val Phe Leu Phe Phe Leu Ser Val Thr Thr Gly
1 5 10 15
Val His Ser Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp
20 25 30
Leu His Trp Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro
35 40 45
Asp Ser Cys Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met
50 55 60
Val Ser Glu Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp
65 70 75 80
Asp Cys Trp Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala
85 90 95
Asp Pro Gln Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val
100 105 110
His Ser Lys Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys
115 120 125
Thr Cys Ala Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala
130 135 140
Gln Thr Phe Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys
145 150 155 160
Tyr Cys Asp Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser
165 170 175
Leu Ala Leu Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp
180 185 190
Pro Leu Tyr Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg
195 200 205
Gln Tyr Cys Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp
210 215 220
Ala Ser Ile Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg
225 230 235 240
Ile Val Asp Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu
245 250 255
Val Ile Gly Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met
260 265 270
Ala Leu Trp Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu
275 280 285
Arg His Ile Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val
290 295 300
Ile Ala Ile Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg
305 310 315 320
Gln Gly Asp Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala
325 330 335
Trp Ala Val Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser
340 345 350
Tyr Thr Ile Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro
355 360 365
Ala Cys Phe Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe
370 375 380
Tyr Glu Trp Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr
385 390 395 400
Val Leu Leu Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu
405 410 415
Leu
<210> 461
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 461
ctggacaatg gattggcaag gacgcctcca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc tgatggtctc tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aattatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaaaag tataaagagt atcttggact ggacatcttt caaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggaa tcagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcgatgc ctgggctgta 960
gctattataa accggcagga gattggtgga cctcgctctt ataccatcgc agttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctact ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt 1194
<210> 462
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 462
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu
385 390 395
<210> 463
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 463
ctggacaatg gattggcaag gacgcctcca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc ggatggtctc tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aattatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaaaag tataaagagt atcttggact ggacatcttt taaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggaa tcagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcttagc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatcgc agttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt 1194
<210> 464
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 464
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Arg Met Val Ser Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val
305 310 315 320
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu
385 390 395
<210> 465
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 465
ctggacaatg gattggcaag gacgcctcca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc ggatggtctc tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aattatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaagag tataaagagt atcttggact ggacatcttt taaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggaa tcagcaagta actcagatgg ccctctgggc tatcatggct 780
ggtcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agaaagggag acaactttga agtgtgggaa cgacctctct caggcgatgc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatcgc agttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt 1194
<210> 466
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 466
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Arg Met Val Ser Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu
385 390 395
<210> 467
<211> 1251
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 467
atggaatgga gctgggtctt tctcttcttc ctgtcagtaa cgactggtgt ccactccctg 60
gacaatggat tggcaaggac gcctccaatg ggctggctgc actgggagcg cttcatgtgc 120
aaccttgact gccaggaaga gccagattcc tgcatcagtg agaagctctt catggagatg 180
gcagagcgga tggtctctga aggctggaag gatgcaggtt atgagtacct ctgcattgat 240
gactgttgga tggctcccca aagagattca gaaggcagac ttcaggcaga ccctcagcgc 300
tttcctcatg ggattcgcca gctagctaat tatgttcaca gcaaaggact gaagctaggg 360
atttatgcag atgttggaaa taaaacctgc gcaggcttcc ctgggagttt tggatactac 420
gacattgatg cccagacctt tgctgactgg ggagtagatc tgctaaaatt tgatggttgt 480
tactgtgaca gtttggaaaa tttggcagat ggttataagc acatgtcctt ggccctgaat 540
aggactggca gaagcattgt gtactcctgt gagtggcctc tttatatgtg gccctttcaa 600
aagcccaatt atacagaaat ccgacagtac tgcaatcact ggcgaaattt tgctgacatt 660
gatgattcct ggaaaagtat aaagagtatc ttggactgga catctttcaa ccaggagaga 720
attgttgatg ttgctggacc agggggttgg aatgacccag atatgttagt gattggcaac 780
tttggcctca gctggaatca gcaagtaact cagatggccc tctgggctat catggctgct 840
cctttattca tgtctaatga cctccgacac atcagccctc aagccaaagc tctccttcag 900
gataaggacg taattgccat caatcaggac cccttgggca agcaagggta ccagcttaga 960
aagggagaca actttgaagt gtgggaacga cctctctcag gcttagcctg ggctgtagct 1020
atgataaacc ggcaggagat tggtggacct cgctcttata ccatccccgt tgcttccctg 1080
ggtaaaggag tggcctgtaa tcctgcctgc ttcatcacac agctcctccc tgtgaaaagg 1140
aagctagggt tctatgaagc tacttcaagg ttaagaagtc acataaatcc cacaggcact 1200
gttttgcttc agctagaaaa tacaatgcag atgtcattaa aagacttact t 1251
<210> 468
<211> 417
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 468
Met Glu Trp Ser Trp Val Phe Leu Phe Phe Leu Ser Val Thr Thr Gly
1 5 10 15
Val His Ser Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp
20 25 30
Leu His Trp Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro
35 40 45
Asp Ser Cys Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Arg Met
50 55 60
Val Ser Glu Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp
65 70 75 80
Asp Cys Trp Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala
85 90 95
Asp Pro Gln Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val
100 105 110
His Ser Lys Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys
115 120 125
Thr Cys Ala Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala
130 135 140
Gln Thr Phe Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys
145 150 155 160
Tyr Cys Asp Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser
165 170 175
Leu Ala Leu Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp
180 185 190
Pro Leu Tyr Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg
195 200 205
Gln Tyr Cys Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp
210 215 220
Lys Ser Ile Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg
225 230 235 240
Ile Val Asp Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu
245 250 255
Val Ile Gly Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met
260 265 270
Ala Leu Trp Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu
275 280 285
Arg His Ile Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val
290 295 300
Ile Ala Ile Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg
305 310 315 320
Lys Gly Asp Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala
325 330 335
Trp Ala Val Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser
340 345 350
Tyr Thr Ile Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro
355 360 365
Ala Cys Phe Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe
370 375 380
Tyr Glu Ala Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr
385 390 395 400
Val Leu Leu Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu
405 410 415
Leu
<210> 469
<211> 1251
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 469
atggaatgga gctgggtctt tctcttcttc ctgtcagtaa cgactggtgt ccactccctg 60
gacaatggat tggcaaggac gcctccaatg ggctggctgc actgggagcg cttcatgtgc 120
aaccttgact gccaggaaga gccagattcc tgcatcagtg agaagctctt catggagatg 180
gcagagcgga tggtcactga aggctggaag gatgcaggtt atgagtacct ctgcattgat 240
gactgttgga tggctcccca aagagattca gaaggcagac ttcaggcaga ccctcagcgc 300
tttcctcatg ggattcgcca gctagctaat tatgttcaca gcaaaggact gaagctaggg 360
atttatgcag atgttggaaa taaaacctgc gcaggcttcc ctgggagttt tggatactac 420
gacattgatg cccagacctt tgctgactgg ggagtagatc tgctaaaatt tgatggttgt 480
tactgtgaca gtttggaaaa tttggcagat ggttataagc acatgtcctt ggccctgaat 540
aggactggca gaagcattgt gtactcctgt gagtggcctc tttatatgtg gccctttcaa 600
aagcccaatt atacagaaat ccgacagtac tgcaatcact ggcgaaattt tgctgacatt 660
gatgattcct ggaaaagtat aaagagtatc ttggactgga catcttttaa ccaggagaga 720
attgttgatg ttgctggacc agggggttgg aatgacccag atatgttagt gattggcaac 780
tttggcctca gctggaacca gcaagtaact cagatggccc tctgggctat catggctggt 840
cctttattca tgtctaatga cctccgacac atcagccctc aagccaaagc tctccttcag 900
gataaggacg taattgccat caatcaggac cccttgggca agcaagggta ccagcttaga 960
aagggagaca actttgaagt gtgggaacga cctctctcag gcctcgcctg ggctgtagct 1020
attataaacc ggcaggagat tggtggacct cgctcttata ccatcgccgt tgcttccctg 1080
ggtaaaggag tggcctgtaa tcctgcctgc ttcatcacac agctcctccc tgtgaaaagg 1140
aagctagggt tctatgaagc tacttcaagg ttaagaagtc acataaatcc cacaggcact 1200
gttttgcttc agctagaaaa tacaatgcag atgtcattaa aagacttact t 1251
<210> 470
<211> 417
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 470
Met Glu Trp Ser Trp Val Phe Leu Phe Phe Leu Ser Val Thr Thr Gly
1 5 10 15
Val His Ser Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp
20 25 30
Leu His Trp Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro
35 40 45
Asp Ser Cys Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Arg Met
50 55 60
Val Thr Glu Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp
65 70 75 80
Asp Cys Trp Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala
85 90 95
Asp Pro Gln Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val
100 105 110
His Ser Lys Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys
115 120 125
Thr Cys Ala Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala
130 135 140
Gln Thr Phe Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys
145 150 155 160
Tyr Cys Asp Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser
165 170 175
Leu Ala Leu Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp
180 185 190
Pro Leu Tyr Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg
195 200 205
Gln Tyr Cys Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp
210 215 220
Lys Ser Ile Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg
225 230 235 240
Ile Val Asp Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu
245 250 255
Val Ile Gly Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met
260 265 270
Ala Leu Trp Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu
275 280 285
Arg His Ile Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val
290 295 300
Ile Ala Ile Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg
305 310 315 320
Lys Gly Asp Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala
325 330 335
Trp Ala Val Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser
340 345 350
Tyr Thr Ile Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro
355 360 365
Ala Cys Phe Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe
370 375 380
Tyr Glu Ala Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr
385 390 395 400
Val Leu Leu Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu
405 410 415
Leu
<210> 471
<211> 1251
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 471
atggaatgga gctgggtctt tctcttcttc ctgtcagtaa cgactggtgt ccactccctg 60
gacaatggat tggcaaggac gcctccaatg ggctggctgc actgggagcg cttcatgtgc 120
aaccttgact gccaggaaga gccagattcc tgcatcagtg agaagctctt catggagatg 180
gcagagcgga tggtcactga aggctggaag gatgcaggtt atgagtacct ctgcattgat 240
gactgttgga tggctcccca aagagattca gaaggcagac ttcaggcaga ccctcagcgc 300
tttcctcatg ggattcgcca gctagctaat catgttcaca gcaaaggact gaagctaggg 360
atttatgcag atgttggaaa taaaacctgc gcaggcttcc ctgggagttt tggatactac 420
gacattgatg cccagacctt tgctgactgg ggagtagatc tgctaaaatt tgatggttgt 480
tactgtgaca gtttggaaaa tttggcagat ggttataagc acatgtcctt ggccctgaat 540
aggactggca gaagcattgt gtactcctgt gagtggcctc tttatatgtg gccctttcaa 600
aagcccaatt atacagaaat ccgacagtac tgcaatcact ggcgaaattt tgctgacatt 660
gatgattcct ggaagagtat aaagagtatc ttggactgga catctttcaa ccaggagaga 720
attgttgatg ttgctggacc agggggttgg aatgacccag atatgttagt gattggcaac 780
tttggcctca gctgggacca gcaagtaact cagatggccc tctgggctat catggctgct 840
cctttattca tgtctaatga cctccgacac atcagccctc aagccaaagc tctccttcag 900
gataaggacg taattgccat caatcaggac cccttgggca agcaagggta ccagcttaga 960
cagggagaca actttgaagt gtgggaacga cctctctcag gcttagcctg ggctgtagct 1020
atgataaacc ggcaggagat tggtggacct cgctcttata ccatcgcagt tgcttccctg 1080
ggtaaaggag tggcctgtaa tcctgcctgc ttcatcacac agctcctccc tgtgaaaagg 1140
aagctagggt tctatgaatg gacttcaagg ttaagaagtc acataaatcc cacaggcact 1200
gttttgcttc agctagaaaa tacaatgcag atgtcattaa aagacttact t 1251
<210> 472
<211> 417
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 472
Met Glu Trp Ser Trp Val Phe Leu Phe Phe Leu Ser Val Thr Thr Gly
1 5 10 15
Val His Ser Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp
20 25 30
Leu His Trp Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro
35 40 45
Asp Ser Cys Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Arg Met
50 55 60
Val Thr Glu Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp
65 70 75 80
Asp Cys Trp Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala
85 90 95
Asp Pro Gln Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val
100 105 110
His Ser Lys Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys
115 120 125
Thr Cys Ala Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala
130 135 140
Gln Thr Phe Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys
145 150 155 160
Tyr Cys Asp Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser
165 170 175
Leu Ala Leu Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp
180 185 190
Pro Leu Tyr Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg
195 200 205
Gln Tyr Cys Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp
210 215 220
Lys Ser Ile Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg
225 230 235 240
Ile Val Asp Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu
245 250 255
Val Ile Gly Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met
260 265 270
Ala Leu Trp Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu
275 280 285
Arg His Ile Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val
290 295 300
Ile Ala Ile Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg
305 310 315 320
Gln Gly Asp Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala
325 330 335
Trp Ala Val Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser
340 345 350
Tyr Thr Ile Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro
355 360 365
Ala Cys Phe Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe
370 375 380
Tyr Glu Trp Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr
385 390 395 400
Val Leu Leu Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu
405 410 415
Leu
<210> 473
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 473
ctggacaatg gattggcaag gacgcctcca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc ggatggtctc tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aatcatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaagag tataaagagt atcttggact ggacatcttt caaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggaa tcagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcgatgc ctgggctgta 960
gctattataa accggcagga gattggtgga cctcgctctt ataccatcgc agttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt 1194
<210> 474
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 474
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Arg Met Val Ser Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu
385 390 395
<210> 475
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 475
ctggacaatg gattggcaag gacgcctcca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcgaggag 120
atggcagagc ggatggtctc tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aattatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaagag tataaagagt atcttggact ggacatcttt caaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggaa ccagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcctcgc ctgggctgta 960
gctattataa accggcagga gattggtgga cctcgctctt ataccatcgc agttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt 1194
<210> 476
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 476
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Ser Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu
385 390 395
<210> 477
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 477
ctggacaatg gattggcaag gacgcctcca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcgaggag 120
atggcagagc tgatggtctc tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aatcatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctgggccag tataaagagt atcttggact ggacatctag gaaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggaa tcagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga gccatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcttagc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatcgc cgttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt 1194
<210> 478
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 478
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Ser Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val
305 310 315 320
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu
385 390 395
<210> 479
<211> 1251
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 479
atggaatgga gctgggtctt tctcttcttc ctgtcagtaa cgactggtgt ccactccctg 60
gacaatggat tggcaaggac gcctccaatg ggctggctgc actgggagcg cttcatgtgc 120
aaccttgact gccaggaaga gccagattcc tgcatcagtg agaagctctt cgaggagatg 180
gcagagctga tggtctctga aggctggaag gatgcaggtt atgagtacct ctgcattgat 240
gactgttgga tggctcccca aagagattca gaaggcagac ttcaggcaga ccctcagcgc 300
tttcctcatg ggattcgcca gctagctaat catgttcaca gcaaaggact gaagctaggg 360
atttatgcag atgttggaaa taaaacctgc gcaggcttcc ctgggagttt tggatactac 420
gacattgatg cccagacctt tgctgactgg ggagtagatc tgctaaaatt tgatggttgt 480
tactgtgaca gtttggaaaa tttggcagat ggttataagc acatgtcctt ggccctgaat 540
aggactggca gaagcattgt gtactcctgt gagtggcctc tttatatgtg gccctttcaa 600
aagcccaatt atacagaaat ccgacagtac tgcaatcact ggcgaaattt tgctgacatt 660
gatgattcct ggaaaagtat aaagagtatc ttggactgga catctttcaa ccaggagaga 720
attgttgatg ttgctggacc agggggttgg aatgacccag atatgttagt gattggcaac 780
tttggcctca gctgggacca gcaagtaact cagatggccc tctgggctat catggctgct 840
cctttattca tgtctaatga cctccgacac atcagccctc aagccaaagc tctccttcag 900
gataaggacg taattgccat caatcaggac cccttgggca agcaagggta ccagcttaga 960
cagggagaca actttgaagt gtgggaacga cctctctcag gcttagcctg ggctgtagct 1020
atgataaacc ggcaggagat tggtggacct cgctcttata ccatcgcagt tgcttccctg 1080
ggtaaaggag tggcctgtaa tcctgcctgc ttcatcacac agctcctccc tgtgaaaagg 1140
aagctagggt tctatgaatg gacttcaagg ttaagaagtc acataaatcc cacaggcact 1200
gttttgcttc agctagaaaa tacaatgcag atgtcattaa aagacttact t 1251
<210> 480
<211> 417
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 480
Met Glu Trp Ser Trp Val Phe Leu Phe Phe Leu Ser Val Thr Thr Gly
1 5 10 15
Val His Ser Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp
20 25 30
Leu His Trp Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro
35 40 45
Asp Ser Cys Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met
50 55 60
Val Ser Glu Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp
65 70 75 80
Asp Cys Trp Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala
85 90 95
Asp Pro Gln Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val
100 105 110
His Ser Lys Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys
115 120 125
Thr Cys Ala Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala
130 135 140
Gln Thr Phe Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys
145 150 155 160
Tyr Cys Asp Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser
165 170 175
Leu Ala Leu Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp
180 185 190
Pro Leu Tyr Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg
195 200 205
Gln Tyr Cys Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp
210 215 220
Lys Ser Ile Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg
225 230 235 240
Ile Val Asp Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu
245 250 255
Val Ile Gly Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met
260 265 270
Ala Leu Trp Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu
275 280 285
Arg His Ile Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val
290 295 300
Ile Ala Ile Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg
305 310 315 320
Gln Gly Asp Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala
325 330 335
Trp Ala Val Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser
340 345 350
Tyr Thr Ile Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro
355 360 365
Ala Cys Phe Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe
370 375 380
Tyr Glu Trp Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr
385 390 395 400
Val Leu Leu Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu
405 410 415
Leu
<210> 481
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 481
ctggacaatg gattggcaag gacgcctcca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcgaggag 120
atggcagagc tgatggtctc tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aatcatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaagag tataaagagt atcttggact ggacatcttt taaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggga ccagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga gccatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcgatgc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatcgc cgttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt 1194
<210> 482
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 482
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Ser Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu
385 390 395
<210> 483
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 483
ctggacaatg gattggcaag gacgcctcca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc tcatggtctc agaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aattatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaaaag tataaagagt atcttggact ggacatcttt taaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggaa tcagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agaaagggag acaactttga agtgtgggaa cgacctctct caggcttagc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatcgc cgttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt 1194
<210> 484
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 484
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val
305 310 315 320
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu
385 390 395
<210> 485
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 485
ctggacaatg gattggcaag gacgcctcca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc tcatggtctc tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aattatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaaaag tataaagagt atcttggact ggacatcttt taaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggaa tcagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agaaagggag acaactttga agtgtgggaa cgacctctct caggcgatgc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatcgc agttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt 1194
<210> 486
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 486
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu
385 390 395
<210> 487
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 487
ctggacaatg gattggcaag gacgcctcca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc tcatggtctc agaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aattatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaaaag tataaagagt atcttggact ggacatcttt taaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggaa ccagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agaaagggag acaactttga agtgtgggaa cgacctctct caggcctcgc ctgggctgta 960
gctattataa accggcagga gattggtgga cctcgctctt ataccatccc cgttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt 1194
<210> 488
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 488
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu
385 390 395
<210> 489
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 489
ctggacaatg gattggcaag gacgcctcca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc tgatggtcac tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aattatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaagag tataaagagt atcttggact ggacatctag gaaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggaa ccagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcctcgc ctgggctgta 960
gctattataa accggcagga gattggtgga cctcgctctt ataccatcgc cgttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt 1194
<210> 490
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 490
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu
385 390 395
<210> 491
<211> 1251
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 491
atggaatgga gctgggtctt tctcttcttc ctgtcagtaa cgactggtgt ccactccctg 60
gacaatggat tggcaaggac gcctccaatg ggctggctgc actgggagcg cttcatgtgc 120
aaccttgact gccaggaaga gccagattcc tgcatcagtg agaagctctt catggagatg 180
gcagagctca tggtcactga aggctggaag gatgcaggtt atgagtacct ctgcattgat 240
gactgttgga tggctcccca aagagattca gaaggcagac ttcaggcaga ccctcagcgc 300
tttcctcatg ggattcgcca gctagctaat tatgttcaca gcaaaggact gaagctaggg 360
atttatgcag atgttggaaa taaaacctgc gcaggcttcc ctgggagttt tggatactac 420
gacattgatg cccagacctt tgctgactgg ggagtagatc tgctaaaatt tgatggttgt 480
tactgtgaca gtttggaaaa tttggcagat ggttataagc acatgtcctt ggccctgaat 540
aggactggca gaagcattgt gtactcctgt gagtggcctc tttatatgtg gccctttcaa 600
aagcccaatt atacagaaat ccgacagtac tgcaatcact ggcgaaattt tgctgacatt 660
gatgattcct ggaaaagtat aaagagtatc ttggactgga catctaggaa ccaggagaga 720
attgttgatg ttgctggacc agggggttgg aatgacccag atatgttagt gattggcaac 780
tttggcctca gctgggacca gcaagtaact cagatggccc tctgggctat catggctgct 840
cctttattca tgtctaatga cctccgacac atcagccctc aagccaaagc tctccttcag 900
gataaggacg taattgccat caatcaggac cccttgggca agcaagggta ccagcttaga 960
cagggagaca actttgaagt gtgggaacga cctctctcag gcgatgcctg ggctgtagct 1020
atgataaacc ggcaggagat tggtggacct cgctcttata ccatcgcagt tgcttccctg 1080
ggtaaaggag tggcctgtaa tcctgcctgc ttcatcacac agctcctccc tgtgaaaagg 1140
aagctagggt tctatgaatg gacttcaagg ttaagaagtc acataaatcc cacaggcact 1200
gttttgcttc agctagaaaa tacaatgcag acctcattaa aagacttact t 1251
<210> 492
<211> 417
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 492
Met Glu Trp Ser Trp Val Phe Leu Phe Phe Leu Ser Val Thr Thr Gly
1 5 10 15
Val His Ser Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp
20 25 30
Leu His Trp Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro
35 40 45
Asp Ser Cys Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met
50 55 60
Val Thr Glu Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp
65 70 75 80
Asp Cys Trp Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala
85 90 95
Asp Pro Gln Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val
100 105 110
His Ser Lys Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys
115 120 125
Thr Cys Ala Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala
130 135 140
Gln Thr Phe Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys
145 150 155 160
Tyr Cys Asp Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser
165 170 175
Leu Ala Leu Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp
180 185 190
Pro Leu Tyr Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg
195 200 205
Gln Tyr Cys Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp
210 215 220
Lys Ser Ile Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg
225 230 235 240
Ile Val Asp Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu
245 250 255
Val Ile Gly Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met
260 265 270
Ala Leu Trp Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu
275 280 285
Arg His Ile Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val
290 295 300
Ile Ala Ile Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg
305 310 315 320
Gln Gly Asp Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala
325 330 335
Trp Ala Val Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser
340 345 350
Tyr Thr Ile Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro
355 360 365
Ala Cys Phe Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe
370 375 380
Tyr Glu Trp Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr
385 390 395 400
Val Leu Leu Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu
405 410 415
Leu
<210> 493
<211> 1251
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 493
atggaatgga gctgggtctt tctcttcttc ctgtcagtaa cgactggtgt ccactccctg 60
gacaatggat tggcaaggac gcctccaatg ggctggctgc actgggagcg cttcatgtgc 120
aaccttgact gccaggaaga gccagattcc tgcatcagtg agaagctctt catggagatg 180
gcagagctga tggtcactga aggctggaag gatgcaggtt atgagtacct ctgcattgat 240
gactgttgga tggctcccca aagagattca gaaggcagac ttcaggcaga ccctcagcgc 300
tttcctcatg ggattcgcca gctagctaat tatgttcaca gcaaaggact gaagctaggg 360
atttatgcag atgttggaaa taaaacctgc gcaggcttcc ctgggagttt tggatactac 420
gacattgatg cccagacctt tgctgactgg ggagtagatc tgctaaaatt tgatggttgt 480
tactgtgaca gtttggaaaa tttggcagat ggttataagc acatgtcctt ggccctgaat 540
aggactggca gaagcattgt gtactcctgt gagtggcctc tttatatgtg gccctttcaa 600
aagcccaatt atacagaaat ccgacagtac tgcaatcact ggcgaaattt tgctgacatt 660
gatgattcct ggaaaagtat aaagagtatc ttggactgga catcttttaa ccaggagaga 720
attgttgatg ttgctggacc agggggttgg aatgacccag atatgttagt gattggcaac 780
tttggcctca gctggaatca gcaagtaact cagatggccc tctgggctat catggctgct 840
cctttattca tgtctaatga cctccgagcc atcagccctc aagccaaagc tctccttcag 900
gataaggacg taattgccat caatcaggac cccttgggca agcaagggta ccagcttaga 960
cagggagaca actttgaagt gtgggaacga cctctctcag gcttagcctg ggctgtagct 1020
atgataaacc ggcaggagat tggtggacct cgctcttata ccatcgcagt tgcttccctg 1080
ggtaaaggag tggcctgtaa tcctgcctgc ttcatcacac agctcctccc tgtgaaaagg 1140
aagctagggt tctatgaatg gacttcaagg ttaagaagtc acataaatcc cacaggcact 1200
gttttgcttc agctagaaaa tacaatgcag atgtcattaa aagacttact t 1251
<210> 494
<211> 417
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 494
Met Glu Trp Ser Trp Val Phe Leu Phe Phe Leu Ser Val Thr Thr Gly
1 5 10 15
Val His Ser Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp
20 25 30
Leu His Trp Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro
35 40 45
Asp Ser Cys Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met
50 55 60
Val Thr Glu Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp
65 70 75 80
Asp Cys Trp Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala
85 90 95
Asp Pro Gln Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val
100 105 110
His Ser Lys Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys
115 120 125
Thr Cys Ala Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala
130 135 140
Gln Thr Phe Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys
145 150 155 160
Tyr Cys Asp Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser
165 170 175
Leu Ala Leu Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp
180 185 190
Pro Leu Tyr Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg
195 200 205
Gln Tyr Cys Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp
210 215 220
Lys Ser Ile Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg
225 230 235 240
Ile Val Asp Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu
245 250 255
Val Ile Gly Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met
260 265 270
Ala Leu Trp Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu
275 280 285
Arg Ala Ile Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val
290 295 300
Ile Ala Ile Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg
305 310 315 320
Gln Gly Asp Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala
325 330 335
Trp Ala Val Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser
340 345 350
Tyr Thr Ile Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro
355 360 365
Ala Cys Phe Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe
370 375 380
Tyr Glu Trp Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr
385 390 395 400
Val Leu Leu Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu
405 410 415
Leu
<210> 495
<211> 1251
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 495
atggaatgga gctgggtctt tctcttcttc ctgtcagtaa cgactggtgt ccactccctg 60
gacaatggat tggcaaggac gcctccaatg ggctggctgc actgggagcg cttcatgtgc 120
aaccttgact gccaggaaga gccagattcc tgcatcagtg agaagctctt catggagatg 180
gcagagctca tggtctcaga aggctggaag gatgcaggtt atgagtacct ctgcattgat 240
gactgttgga tggctcccca aagagattca gaaggcagac ttcaggcaga ccctcagcgc 300
tttcctcatg ggattcgcca gctagctaat tatgttcaca gcaaaggact gaagctaggg 360
atttatgcag atgttggaaa taaaacctgc gcaggcttcc ctgggagttt tggatactac 420
gacattgatg cccagacctt tgctgactgg ggagtagatc tgctaaaatt tgatggttgt 480
tactgtgaca gtttggaaaa tttggcagat ggttataagc acatgtcctt ggccctgaat 540
aggactggca gaagcattgt gtactcctgt gagtggcctc tttatatgtg gccctttcaa 600
aagcccaatt atacagaaat ccgacagtac tgcaatcact ggcgaaattt tgctgacatt 660
gatgattcct ggaagagtat aaagagtatc ttggactgga catcttttaa ccaggagaga 720
attgttgatg ttgctggacc agggggttgg aatgacccag atatgttagt gattggcaac 780
tttggcctca gctggaacca gcaagtaact cagatggccc tctgggctat catggctgct 840
cctttattca tgtctaatga cctccgacac atcagccctc aagccaaagc tctccttcag 900
gataaggacg taattgccat caatcaggac cccttgggca agcaagggta ccagcttaga 960
cagggagaca actttgaagt gtgggaacga cctctctcag gcttagcctg ggctgtagct 1020
atgataaacc ggcaggagat tggtggacct cgctcttata ccatcgcagt tgcttccctg 1080
ggtaaaggag tggcctgtaa tcctgcctgc ttcatcacac agctcctccc tgtgaaaagg 1140
aagctagggt tctatgaagc tacttcaagg ttaagaagtc acataaatcc cacaggcact 1200
gttttgcttc agctagaaaa tacaatgcag atgtcattaa aagacttact t 1251
<210> 496
<211> 417
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 496
Met Glu Trp Ser Trp Val Phe Leu Phe Phe Leu Ser Val Thr Thr Gly
1 5 10 15
Val His Ser Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp
20 25 30
Leu His Trp Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro
35 40 45
Asp Ser Cys Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met
50 55 60
Val Ser Glu Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp
65 70 75 80
Asp Cys Trp Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala
85 90 95
Asp Pro Gln Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val
100 105 110
His Ser Lys Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys
115 120 125
Thr Cys Ala Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala
130 135 140
Gln Thr Phe Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys
145 150 155 160
Tyr Cys Asp Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser
165 170 175
Leu Ala Leu Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp
180 185 190
Pro Leu Tyr Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg
195 200 205
Gln Tyr Cys Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp
210 215 220
Lys Ser Ile Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg
225 230 235 240
Ile Val Asp Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu
245 250 255
Val Ile Gly Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met
260 265 270
Ala Leu Trp Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu
275 280 285
Arg His Ile Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val
290 295 300
Ile Ala Ile Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg
305 310 315 320
Gln Gly Asp Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala
325 330 335
Trp Ala Val Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser
340 345 350
Tyr Thr Ile Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro
355 360 365
Ala Cys Phe Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe
370 375 380
Tyr Glu Ala Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr
385 390 395 400
Val Leu Leu Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu
405 410 415
Leu
<210> 497
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 497
ctggacaatg gattggcaag gacgcctcca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc tcatggtctc agaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aatcatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaagag tataaagagt atcttggact ggacatcttt caaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggaa tcagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcttagc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatcgc agttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt 1194
<210> 498
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 498
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val
305 310 315 320
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu
385 390 395
<210> 499
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 499
ctggacaatg gattggcaag gacgcctcca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc tgatggtctc tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aatcatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaaaag tataaagagt atcttggact ggacatctag gaaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggaa tcagcaagta actcagatgg ccctctgggc tatcatggct 780
ggtcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agaaagggag acaactttga agtgtgggaa cgacctctct caggcctcgc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatccc cgttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt 1194
<210> 500
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 500
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val
305 310 315 320
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu
385 390 395
<210> 501
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 501
ctggacaatg gattggcaag gacgcctcca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc tgatggtctc tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aatcatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctgggccag tataaagagt atcttggact ggacatctag gaaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggga ccagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcttagc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatcgc agttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt 1194
<210> 502
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 502
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val
305 310 315 320
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu
385 390 395
<210> 503
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 503
ctggacaatg gattggcaag gacgcctcca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc tgatggtctc tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aatcatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctgggccag tataaagagt atcttggact ggacatcttt taaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggga ccagcaagta actcagatgg ccctatgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcgatgc ctgggctgta 960
gctattataa accggcagga gattggtgga cctcgctctt ataccatcgc cgttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagacctcat taaaagactt actt 1194
<210> 504
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 504
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 505
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 505
ctggacaatg gattggcaag gacgcctcca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc tcatggtctc agaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aatcatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctgggccag tataaagagt atcttggact ggacatcttt taaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggga ccagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcctcgc ctgggctgta 960
gctattataa accggcagga gattggtgga cctcgctctt ataccatcgc cgttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga agctacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt 1194
<210> 506
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 506
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu
385 390 395
<210> 507
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 507
ctggacaatg gattggcaag gacgcctaca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc tgatggtctc tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aattatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaaaag tataaagagt atcttggact ggacatcttt taaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggaa tcagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcttagc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatcgc agttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga agctacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt 1194
<210> 508
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 508
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val
305 310 315 320
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu
385 390 395
<210> 509
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 509
ctggacaatg gattggcaag gacgcctaca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc tgatggtctc tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aatcatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaagag tataaagagt atcttggact ggacatctag gaaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggaa tcagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga gccatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcttagc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatcgc agttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt 1194
<210> 510
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 510
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val
305 310 315 320
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu
385 390 395
<210> 511
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 511
ctggacaatg gattggcaag gacgcctacc atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc tgatggtctc tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aatcatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaaaag tataaagagt atcttggact ggacatcttt taaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggaa tcagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga gccatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcttagc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatccc cgttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt 1194
<210> 512
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 512
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val
305 310 315 320
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu
385 390 395
<210> 513
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 513
ctggacaatg gattggcaag gacgcctaca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc tgatggtctc tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aatcatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaagag tataaagagt atcttggact ggacatcttt caaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggaa tcagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcgatgc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatcgc agttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt 1194
<210> 514
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 514
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu
385 390 395
<210> 515
<211> 1251
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 515
atggaatgga gctgggtctt tctcttcttc ctgtcagtaa cgactggtgt ccactccctg 60
gacaatggat tggcaaggac gcctacaatg ggctggctgc actgggagcg cttcatgtgc 120
aaccttgact gccaggaaga gccagattcc tgcatcagtg agaagctctt catggagatg 180
gcagagctca tggtctcaga aggctggaag gatgcaggtt atgagtacct ctgcattgat 240
gactgttgga tggctcccca aagagattca gaaggcagac ttcaggcaga ccctcagcgc 300
tttcctcatg ggattcgcca gctagctaat catgttcaca gcaaaggact gaagctaggg 360
atttatgcag atgttggaaa taaaacctgc gcaggcttcc ctgggagttt tggatactac 420
gacattgatg cccagacctt tgctgactgg ggagtagatc tgctaaaatt tgatggttgt 480
tactgtgaca gtttggaaaa tttggcagat ggttataagc acatgtcctt ggccctgaat 540
aggactggca gaagcattgt gtactcctgt gagtggcctc tttatatgtg gccctttcaa 600
aagcccaatt atacagaaat ccgacagtac tgcaatcact ggcgaaattt tgctgacatt 660
gatgattcct ggaagagtat aaagagtatc ttggactgga catcttttaa ccaggagaga 720
attgttgatg ttgctggacc agggggttgg aatgacccag atatgttagt gattggcaac 780
tttggcctca gctgggacca gcaagtaact cagatggccc tctgggctat catggctgct 840
cctttattca tgtctaatga cctccgacac atcagccctc aagccaaagc tctccttcag 900
gataaggacg taattgccat caatcaggac cccttgggca agcaagggta ccagcttaga 960
cagggagaca actttgaagt gtgggaacga cctctctcag gcttagcctg ggctgtagct 1020
atgataaacc ggcaggagat tggtggacct cgctcttata ccatcgccgt tgcttccctg 1080
ggtaaaggag tggcctgtaa tcctgcctgc ttcatcacac agctcctccc tgtgaaaagg 1140
aagctagggt tctatgaatg gacttcaagg ttaagaagtc acataaatcc cacaggcact 1200
gttttgcttc agctagaaaa tacaatgcag atgtcattaa aagacttact t 1251
<210> 516
<211> 417
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 516
Met Glu Trp Ser Trp Val Phe Leu Phe Phe Leu Ser Val Thr Thr Gly
1 5 10 15
Val His Ser Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp
20 25 30
Leu His Trp Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro
35 40 45
Asp Ser Cys Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met
50 55 60
Val Ser Glu Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp
65 70 75 80
Asp Cys Trp Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala
85 90 95
Asp Pro Gln Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val
100 105 110
His Ser Lys Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys
115 120 125
Thr Cys Ala Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala
130 135 140
Gln Thr Phe Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys
145 150 155 160
Tyr Cys Asp Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser
165 170 175
Leu Ala Leu Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp
180 185 190
Pro Leu Tyr Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg
195 200 205
Gln Tyr Cys Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp
210 215 220
Lys Ser Ile Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg
225 230 235 240
Ile Val Asp Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu
245 250 255
Val Ile Gly Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met
260 265 270
Ala Leu Trp Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu
275 280 285
Arg His Ile Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val
290 295 300
Ile Ala Ile Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg
305 310 315 320
Gln Gly Asp Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala
325 330 335
Trp Ala Val Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser
340 345 350
Tyr Thr Ile Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro
355 360 365
Ala Cys Phe Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe
370 375 380
Tyr Glu Trp Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr
385 390 395 400
Val Leu Leu Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu
405 410 415
Leu
<210> 517
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 517
ctggacaatg gattggcaag gacgcctaca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc tgatggtctc tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aatcatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaaaag tataaagagt atcttggact ggacatcttt caaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggga ccagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga gccatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcctcgc ctgggctgta 960
gctattataa accggcagga gattggtgga cctcgctctt ataccatcgc agttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt 1194
<210> 518
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 518
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu
385 390 395
<210> 519
<211> 1251
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 519
atggaatgga gctgggtctt tctcttcttc ctgtcagtaa cgactggtgt ccactccctg 60
gacaatggat tggcaaggac gcctacaatg ggctggctgc actgggagcg cttcatgtgc 120
aaccttgact gccaggaaga gccagattcc tgcatcagtg agaagctctt catggagatg 180
gcagagctca tggtctcaga aggctggaag gatgcaggtt atgagtacct ctgcattgat 240
gactgttgga tggctcccca aagagattca gaaggcagac ttcaggcaga ccctcagcgc 300
tttcctcatg ggattcgcca gctagctaat catgttcaca gcaaaggact gaagctaggg 360
atttatgcag atgttggaaa taaaacctgc gcaggcttcc ctgggagttt tggatactac 420
gacattgatg cccagacctt tgctgactgg ggagtagatc tgctaaaatt tgatggttgt 480
tactgtgaca gtttggaaaa tttggcagat ggttataagc acatgtcctt ggccctgaat 540
aggactggca gaagcattgt gtactcctgt gagtggcctc tttatatgtg gccctttcaa 600
aagcccaatt atacagaaat ccgacagtac tgcaatcact ggcgaaattt tgctgacatt 660
gatgattcct ggaaaagtat aaagagtatc ttggactgga catcttttaa ccaggagaga 720
attgttgatg ttgctggacc agggggttgg aatgacccag atatgttagt gattggcaac 780
tttggcctca gctgggacca gcaagtaact cagatggccc tctgggctat catggctgct 840
cctttattca tgtctaatga cctccgacac atcagccctc aagccaaagc tctccttcag 900
gataaggacg taattgccat caatcaggac cccttgggca agcaagggta ccagcttaga 960
aagggagaca actttgaagt gtgggaacga cctctctcag gcttagcctg ggctgtagct 1020
attataaacc ggcaggagat tggtggacct cgctcttata ccatccccgt tgcttccctg 1080
ggtaaaggag tggcctgtaa tcctgcctgc ttcatcacac agctcctccc tgtgaaaagg 1140
aagctagggt tctatgaatg gacttcaagg ttaagaagtc acataaatcc cacaggcact 1200
gttttgcttc agctagaaaa tacaatgcag atgtcattaa aagacttact t 1251
<210> 520
<211> 417
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 520
Met Glu Trp Ser Trp Val Phe Leu Phe Phe Leu Ser Val Thr Thr Gly
1 5 10 15
Val His Ser Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp
20 25 30
Leu His Trp Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro
35 40 45
Asp Ser Cys Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met
50 55 60
Val Ser Glu Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp
65 70 75 80
Asp Cys Trp Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala
85 90 95
Asp Pro Gln Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val
100 105 110
His Ser Lys Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys
115 120 125
Thr Cys Ala Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala
130 135 140
Gln Thr Phe Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys
145 150 155 160
Tyr Cys Asp Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser
165 170 175
Leu Ala Leu Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp
180 185 190
Pro Leu Tyr Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg
195 200 205
Gln Tyr Cys Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp
210 215 220
Lys Ser Ile Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg
225 230 235 240
Ile Val Asp Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu
245 250 255
Val Ile Gly Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met
260 265 270
Ala Leu Trp Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu
275 280 285
Arg His Ile Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val
290 295 300
Ile Ala Ile Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg
305 310 315 320
Lys Gly Asp Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala
325 330 335
Trp Ala Val Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser
340 345 350
Tyr Thr Ile Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro
355 360 365
Ala Cys Phe Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe
370 375 380
Tyr Glu Trp Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr
385 390 395 400
Val Leu Leu Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu
405 410 415
Leu
<210> 521
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 521
ctggacaatg gattggcaag gacgcctaca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc tcatggtctc agaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aatcatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaaaag tataaagagt atcttggact ggacatcttt caaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggaa ccagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agaaagggag acaactttga agtgtgggaa cgacctctct caggcctcgc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatcgc agttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt 1194
<210> 522
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 522
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val
305 310 315 320
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu
385 390 395
<210> 523
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 523
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttatggaa 120
atggccgaac tgatggtgtc cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattgc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 524
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 524
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 525
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 525
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac tgatggtgac cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gctatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 526
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 526
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 527
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 527
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaagaa 120
atggccgaac tgatggtgtc cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacctcac tgaaggacct cctg 1194
<210> 528
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 528
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Ser Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 529
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 529
ctggataatg gattggctag aacacctccc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catggaagtc cataaagagc atcttggact ggaccagttt taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccataatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 530
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 530
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 531
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 531
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttatggaa 120
atggccgaac ggatggtgtc cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc gctatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccataatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agcaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagatgtcac tgaaggacct cctg 1194
<210> 532
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 532
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Arg Met Val Ser Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu
385 390 395
<210> 533
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 533
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttatggaa 120
atggccgaac ggatggtgac cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat gggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
caaaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc catatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggactcgc gtgggctgtg 960
gccatgatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agcaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacctcac tgaaggacct cctg 1194
<210> 534
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 534
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Gly Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val
305 310 315 320
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 535
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 535
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttatggaa 120
atggccgaac ggatggtgtc cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagttt taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggaa tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaagggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga atggaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 536
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 536
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Arg Met Val Ser Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 537
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 537
ctggataatg gattggctag aacacctccc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac ggatggtgac cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggaa tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaagggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agcaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacctcac tgaaggacct cctg 1194
<210> 538
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 538
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 539
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 539
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagttt taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaagggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agcaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 540
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 540
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 541
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 541
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac ggatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catggaagtc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gctatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaagggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agcaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacctcac tgaaggacct cctg 1194
<210> 542
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 542
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 543
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 543
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggactcgc gtgggctgtg 960
gccataatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagatgtcac tgaaggacct cctg 1194
<210> 544
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 544
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu
385 390 395
<210> 545
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 545
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttatggaa 120
atggccgaac tgatggtgac cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
gcccctcttt tcatgagcaa cgatctgcgc gctatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggactcgc gtgggctgtg 960
gccatgatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 546
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 546
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val
305 310 315 320
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 547
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 547
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttatggaa 120
atggccgaac tgatggtgac cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc gctatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agacagggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccatgatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacctcac tgaaggacct cctg 1194
<210> 548
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 548
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 549
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 549
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttatggaa 120
atggccgaac tgatggtgac cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagttt taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc gctatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agacagggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccatgatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 550
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 550
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 551
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 551
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttatggaa 120
atggccgaac tgatggtgtc cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggactcgc gtgggctgtg 960
gccataatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 552
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 552
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 553
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 553
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttatggaa 120
atggccgaac tgatggtgtc cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catggaagtc cataaagagc atcttggact ggaccagttt taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccataatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 554
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 554
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 555
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 555
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agacagggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccataatta atcggcaaga gatcggaggt ccacgctcct atacaattgc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agcaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacctcac tgaaggacct cctg 1194
<210> 556
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 556
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 557
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 557
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaagaa 120
atggccgaac tgatggtgac cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagttt taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggaa tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc catatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agacagggcg acaactttga ggtctgggaa aggcctttga gtggactcgc gtgggctgtg 960
gccataatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagatgtcac tgaaggacct cctg 1194
<210> 558
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 558
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu
385 390 395
<210> 559
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 559
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac tgatggtgtc cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc catatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccatgatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga atggaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 560
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 560
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Ser Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 561
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 561
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac tgatggtgtc cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagttt taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc catatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaagggcg acaactttga ggtctgggaa aggcctttga gtggactcgc gtgggctgtg 960
gccatgatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agcaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacctcac tgaaggacct cctg 1194
<210> 562
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 562
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Ser Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val
305 310 315 320
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 563
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 563
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaagaa 120
atggccgaac tgatggtgtc cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaagggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagatgtcac tgaaggacct cctg 1194
<210> 564
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 564
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Ser Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu
385 390 395
<210> 565
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 565
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaagaa 120
atggccgaac ggatggtgtc cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc catatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaagggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 566
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 566
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Ser Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 567
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 567
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac ggatggtgtc cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catggaagtc cataaagagc atcttggact ggaccagttt taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggaa tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc gctatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccataatta atcggcaaga gatcggaggt ccacgctcct atacaattgc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agcaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacctcac tgaaggacct cctg 1194
<210> 568
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 568
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Ser Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 569
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 569
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttatggaa 120
atggccgaac tgatggtgac cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc catatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattgc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga atggaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagatgtcac tgaaggacct cctg 1194
<210> 570
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 570
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu
385 390 395
<210> 571
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 571
ctggataatg gattggctag aacacctccc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttatggaa 120
atggccgaac tgatggtgtc cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catggaagtc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccataatta atcgccaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagatgtcac tgaaggacct cctg 1194
<210> 572
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 572
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu
385 390 395
<210> 573
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 573
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catggaagtc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccatgatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agcaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 574
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 574
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 575
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 575
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttatggaa 120
atggccgaac tgatggtgac cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agacagggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccatgatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agcaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 576
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 576
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 577
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 577
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttatggaa 120
atggccgaac tgatggtgac cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagatgtcac tgaaggacct cctg 1194
<210> 578
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 578
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu
385 390 395
<210> 579
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 579
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac tgatggtgtc cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaagggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 580
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 580
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Ser Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 581
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 581
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggaa tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc gctatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaagggcg acaactttga ggtctgggaa aggcctttga gtggactcgc gtgggctgtg 960
gccataatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 582
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 582
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 583
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 583
ctggataatg gattggctag aacacctccc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaagggcg acaactttga ggtctgggaa aggcctttga gtggactcgc gtgggctgtg 960
gccataatta atcggcaaga gatcggaggt ccacgctcct atacaattgc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agcaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagatgtcac tgaaggacct cctg 1194
<210> 584
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 584
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu
385 390 395
<210> 585
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 585
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaaaatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagttt taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc gctatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agacagggcg acaactttga ggtctgggaa aggcctttga gtggactcgc gtgggctgtg 960
gccataatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacctcac tgaaggacct cctg 1194
<210> 586
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 586
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asn Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 587
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 587
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catggaagtc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc catatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggactcgc gtgggctgtg 960
gccataatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 588
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 588
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 589
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 589
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
gcccctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattgc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agcaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagatgtcac tgaaggacct cctg 1194
<210> 590
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 590
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu
385 390 395
<210> 591
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 591
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagatgct ctttgaagaa 120
atggccgaac ggatggtgac cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggaa tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
gcccctcttt tcatgagcaa cgatctgcgc gctatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggactcgc gtgggctgtg 960
gccataatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagatgtcac tgaaggacct cctg 1194
<210> 592
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 592
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Met Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu
385 390 395
<210> 593
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 593
ctggataatg gattggctag aacacctccc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaagaa 120
atggccgaac ggatggtgac cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagttt taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc catatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaagggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattgc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacctcac tgaaggacct cctg 1194
<210> 594
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 594
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 595
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 595
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccataatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga atggaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 596
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 596
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 597
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 597
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttatggaa 120
atggccgaac ggatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagttt taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggaa tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc catatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agcaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacctcac tgaaggacct cctg 1194
<210> 598
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 598
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 599
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 599
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac tgatggtgtc cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
gcccctcttt tcatgagcaa cgatctgcgc catatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 600
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 600
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Ser Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 601
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 601
ctggataatg gattggctag aacacctccc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac tgatggtgac cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gctatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggactcgc gtgggctgtg 960
gccatgatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagatgtcac tgaaggacct cctg 1194
<210> 602
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 602
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val
305 310 315 320
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu
385 390 395
<210> 603
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 603
ctggataatg gattggctag aacacctccc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttatggaa 120
atggccgaac tgatggtgtc cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catggaagtc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaagggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattgc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga atggaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagatgtcac tgaaggacct cctg 1194
<210> 604
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 604
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu
385 390 395
<210> 605
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 605
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac tgatggtgac cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catggaagtc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaagggcg acaactttga ggtctgggaa aggcctttga gtggactcgc gtgggctgtg 960
gccatgatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 606
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 606
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val
305 310 315 320
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 607
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 607
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttatggaa 120
atggccgaac tgatggtgac cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc catatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 608
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 608
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 609
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 609
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttatggaa 120
atggccgaac ggatggtgtc cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggaa tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 610
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 610
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Arg Met Val Ser Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 611
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 611
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac ggatggtgac cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catggaagtc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggaa tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
gcccctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 612
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 612
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 613
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 613
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttatggaa 120
atggccgaac tgatggtgac cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagatgtcac tgaaggacct cctg 1194
<210> 614
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 614
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu
385 390 395
<210> 615
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 615
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac tgatggtgac cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agacagggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 616
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 616
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 617
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 617
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaagaa 120
atggccgaac ggatggtgtc cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc catatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agacagggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccataatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 618
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 618
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Ser Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 619
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 619
ctggataatg gattggctag aacacctccc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac tgatggtgtc cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc catatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccataatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 620
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 620
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Ser Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 621
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 621
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttatggaa 120
atggccgaac tgatggtgtc cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catggaagtc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggaa tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
gcccctcttt tcatgagcaa cgatctgcgc gctatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccataatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 622
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 622
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 623
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 623
ctggataatg gattggctag aacacctccc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttatggaa 120
atggccgaac tgatggtgtc cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catggaagtc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagatgtcac tgaaggacct cctg 1194
<210> 624
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 624
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu
385 390 395
<210> 625
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 625
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttatggaa 120
atggccgaac ggatggtgtc cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gctatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaagggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattgc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agcaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 626
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 626
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Arg Met Val Ser Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 627
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 627
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac ggatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagttt taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggaa tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
gcccctcttt tcatgagcaa cgatctgcgc catatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaagggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 628
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 628
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 629
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 629
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac ggatggtgtc cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaagggcg acaactttga ggtctgggaa aggcctttga gtggactcgc gtgggctgtg 960
gccataatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agcaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 630
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 630
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Ser Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 631
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 631
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac ggatggtgtc cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggactcgc gtgggctgtg 960
gccatgatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agcaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagatgtcac tgaaggacct cctg 1194
<210> 632
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 632
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Ser Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val
305 310 315 320
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu
385 390 395
<210> 633
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 633
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagttt taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaagggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagatgtcac tgaaggacct cctg 1194
<210> 634
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 634
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu
385 390 395
<210> 635
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 635
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttatggaa 120
atggccgaac ggatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagttt taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
gcccctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggactcgc gtgggctgtg 960
gccataatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga atggaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 636
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 636
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 637
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 637
ctggataatg gattggctag aacacctccc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttatggaa 120
atggccgaac ggatggtgtc cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagttt taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
gcccctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccataatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagatgtcac tgaaggacct cctg 1194
<210> 638
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 638
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Arg Met Val Ser Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu
385 390 395
<210> 639
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 639
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttatggaa 120
atggccgaac ggatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagttt taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc gctatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaagggcg acaactttga ggtctgggaa aggcctttga gtggactcgc gtgggctgtg 960
gccataatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 640
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 640
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 641
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 641
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggactcgc gtgggctgtg 960
gccatgatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga atggaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 642
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 642
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val
305 310 315 320
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 643
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 643
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaagaa 120
atggccgaac ggatggtgac cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggactcgc gtgggctgtg 960
gccataatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agcaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacctcac tgaaggacct cctg 1194
<210> 644
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 644
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 645
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 645
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagttt taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gctatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggactcgc gtgggctgtg 960
gccataatta atcggcaaga gatcggaggt ccacgctcct atacaattgc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agcaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagatgtcac tgaaggacct cctg 1194
<210> 646
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 646
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu
385 390 395
<210> 647
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 647
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagttt taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
gcccctcttt tcatgagcaa cgatctgcgc catatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccataatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagatgtcac tgaaggacct cctg 1194
<210> 648
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 648
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu
385 390 395
<210> 649
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 649
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggactcgc gtgggctgtg 960
gccatgatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 650
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 650
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val
305 310 315 320
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 651
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 651
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccataatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 652
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 652
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 653
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 653
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac ggatggtgac cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaagggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattgc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga atggaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacctcac tgaaggacct cctg 1194
<210> 654
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 654
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 655
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 655
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac ggatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagttt taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccatgatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacctcac tgaaggacct cctg 1194
<210> 656
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 656
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 657
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 657
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac ggatggtgtc cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc catatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggactcgc gtgggctgtg 960
gccataatta atcggcaaga gatcggaggt ccacgctcct atacaattgc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 658
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 658
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Ser Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 659
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 659
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac ggatggtgtc cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc catatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaagggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agcaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 660
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 660
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Ser Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 661
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 661
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggaa tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc catatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaagggcg acaactttga ggtctgggaa aggcctttga gtggactcgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agcaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacctcac tgaaggacct cctg 1194
<210> 662
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 662
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 663
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 663
ctggataatg gattggctag aacacctccc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaaacaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggactcgc gtgggctgtg 960
gccatgatta atcggcaaga gatcggaggt ccacgctcct atacaattgc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacctcac tgaaggacct cctg 1194
<210> 664
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 664
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val
305 310 315 320
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 665
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 665
ctggataatg gattggctag aacacctccc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac ggatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagttt taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc gctatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggactcgc gtgggctgtg 960
gccatgatta atcggcaaga gatcggaggt ccacgctcct atacaattgc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 666
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 666
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val
305 310 315 320
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 667
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 667
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaagaa 120
atggccgaac tgatggtgtc cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggaa tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc catatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccataatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agcaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagatgtcac tgaaggacct cctg 1194
<210> 668
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 668
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Ser Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu
385 390 395
<210> 669
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 669
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac ggatggtgtc cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catggaagtc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacctcac tgaaggacct cctg 1194
<210> 670
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 670
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Ser Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 671
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 671
ctggataatg gattggctag aacacctccc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaagaa 120
atggccgaac ggatggtgtc cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagttt taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc catatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccataatta atcggcaaga gatcggaggt ccacgctcct atacaattgc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacctcac tgaaggacct cctg 1194
<210> 672
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 672
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Ser Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 673
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 673
ctggataatg gattggctag aacacctccc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaagaa 120
atggccgaac tgatggtgtc cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagttt taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc catatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaagggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agcaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 674
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 674
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Ser Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 675
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 675
ctggataatg gattggctag aacacctccc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
gcccctcttt tcatgagcaa cgatctgcgc catatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaagggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agcaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 676
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 676
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 677
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 677
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttatggaa 120
atggccgaac tgatggtgac cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
gcccctcttt tcatgagcaa cgatctgcgc catatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggactcgc gtgggctgtg 960
gccatgatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 678
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 678
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val
305 310 315 320
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 679
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 679
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac ggatggtgac cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
gcccctcttt tcatgagcaa cgatctgcgc catatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagatgtcac tgaaggacct cctg 1194
<210> 680
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 680
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu
385 390 395
<210> 681
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 681
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac ggatggtgtc cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagttt taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggaa tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
gcccctcttt tcatgagcaa cgatctgcgc gctatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccataatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 682
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 682
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Ser Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 683
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 683
ctggataatg gattggctag aacacctccc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttatggaa 120
atggccgaac ggatggtgac cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
gcccctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agacagggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccataatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 684
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 684
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 685
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 685
ctggataatg gattggctag aacacctccc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttatggaa 120
atggccgaac ggatggtgac cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagttt taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagatgtcac tgaaggacct cctg 1194
<210> 686
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 686
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu
385 390 395
<210> 687
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 687
ctggataatg gattggctag aacacctccc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttatggaa 120
atggccgaac ggatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccatgatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agcaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 688
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 688
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 689
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 689
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttatggaa 120
atggccgaac tgatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggaa tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
gcccctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agacagggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattgc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agcaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 690
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 690
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 691
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 691
ctggataatg gattggctag aacacctccc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttatggaa 120
atggccgaac ggatggtgac cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agcaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagatgtcac tgaaggacct cctg 1194
<210> 692
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 692
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu
385 390 395
<210> 693
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 693
ctggataatg gattggctag aacacctccc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttatggaa 120
atggccgaac ggatggtgtc cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggactcgc gtgggctgtg 960
gccatgatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacctcac tgaaggacct cctg 1194
<210> 694
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 694
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Arg Met Val Ser Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val
305 310 315 320
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 695
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 695
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc catatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 696
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 696
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 697
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 697
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttatggaa 120
atggccgaac ggatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaagggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 698
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 698
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 699
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 699
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
gcccctcttt tcatgagcaa cgatctgcgc gctatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccataatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacctcac tgaaggacct cctg 1194
<210> 700
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 700
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 701
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 701
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaagaa 120
atggccgaac ggatggtgac cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
gcccctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggactcgc gtgggctgtg 960
gccatgatta atcggcaaga gatcggaggt ccacgctcct atacaattgc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 702
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 702
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val
305 310 315 320
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 703
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 703
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 704
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 704
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 705
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 705
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggac gtcttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaaccaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatga ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
cgtaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgttctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cttt 1194
<210> 706
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 706
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Thr Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Phe
385 390 395
<210> 707
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 707
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg attgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc aacatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 708
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 708
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Asn Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 709
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 709
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg attgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgcatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc aacatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcgggt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcaatatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga aaatacgatg aacacttcac tgaaggacct cctg 1194
<210> 710
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 710
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Asn Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Asn
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Asn Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 711
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 711
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc aacatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcaatt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 712
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 712
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Asn Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Asn Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 713
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 713
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg attgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc aacatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcaatt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgctttatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga aaatacgaat aacacttcac tgaaggacct cctg 1194
<210> 714
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 714
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Asn Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Asn Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Asn Asn Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 715
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 715
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc aacatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcaatt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg aacacttcac tgaaggacct cctg 1194
<210> 716
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 716
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Asn Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Asn Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Asn Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 717
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 717
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa gaatctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 718
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 718
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Asn Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 719
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 719
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa gaggctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 720
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 720
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Arg Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 721
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 721
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa gagtctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 722
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 722
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ser Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 723
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 723
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatacg tatcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 724
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 724
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Arg Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 725
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 725
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtaggttct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 726
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 726
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Gly Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 727
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 727
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtaaattct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 728
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 728
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Asn Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 729
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 729
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtacagtct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 730
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 730
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Gln Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 731
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 731
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagtttct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 732
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 732
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Val Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 733
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 733
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg attgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgcatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctacagtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcaatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 734
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 734
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Ser Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 735
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 735
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg attgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctacagtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc aacatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcgggt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga aaatacgatg caaacttcac tgaaggacct cctg 1194
<210> 736
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 736
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Ser Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Asn Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 737
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 737
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg attgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctacagtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc aacatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcgggt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcaatatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga aaatacgaat caaacttcac tgaaggacct cctg 1194
<210> 738
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 738
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Ser Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Asn Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Asn
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Asn Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 739
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 739
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg attgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctacagtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcaatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcaatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 740
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 740
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Ser Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asn Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 741
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 741
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg attgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctacagtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcaatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcgggt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgctttatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgaa taatacgatg aacacttcac tgaaggacct cctg 1194
<210> 742
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 742
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Ser Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Asn Asn Thr Met Asn Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 743
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 743
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg attgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctacagtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcaatt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 744
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 744
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Ser Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Asn Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 745
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 745
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg attgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgcatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctacagtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcaatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcaatt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgctttatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgaa taatacgact caaacttcac tgaaggacct cctg 1194
<210> 746
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 746
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Ser Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Asn Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Asn Asn Thr Thr Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 747
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 747
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg attgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctggctatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcaatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcgggt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 748
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 748
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Ala Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 749
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 749
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg attgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctggctatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctacagtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcaatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcgggt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 750
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 750
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Ala Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Ser Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 751
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 751
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg attgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctggctatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctacagtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc aacatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcgggt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgctttatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 752
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 752
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Ala Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Ser Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Asn Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 753
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 753
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctggctatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcaatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcaatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcgggt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 754
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 754
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Ala Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asn Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 755
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 755
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg attgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgactatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgctttatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 756
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 756
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Thr Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 757
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 757
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgactatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctacagtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcaatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcaatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcgccaaga gatcggaggt ccacgcgggt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 758
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 758
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Thr Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Ser Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asn Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 759
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 759
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg attgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgactatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctacagtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcaatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcaatt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 760
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 760
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Thr Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Ser Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Asn Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 761
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 761
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg attgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgactatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcaatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcaatt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgctttatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 762
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 762
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Thr Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asn Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Asn Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 763
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 763
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg attgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctggtaatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc aacatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgctttatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgaa taatacgact caaacttcac tgaaggacct cctg 1194
<210> 764
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 764
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Val Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Asn Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Asn Asn Thr Thr Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 765
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 765
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctggtaatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctacagtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcaatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc aacatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcaatt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgctttatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 766
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 766
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Val Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Ser Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asn Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Asn Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Asn Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 767
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 767
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgagattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 768
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 768
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Glu Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 769
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 769
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tataatattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 770
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 770
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asn Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 771
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 771
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tattcgattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 772
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 772
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Ser Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 773
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 773
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg attgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgcatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcaatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcaatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcgggt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgctttatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 774
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 774
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asn Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 775
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 775
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg attgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcaatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcaatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcaatt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgctttatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga aaatacgatg caaacttcac tgaaggacct cctg 1194
<210> 776
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 776
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asn Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Asn Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 777
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 777
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgt cgtgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc aacatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcgggt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcaatatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 778
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 778
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Ser Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Asn Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Asn
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 779
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 779
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgt cgtgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctacagta acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcaatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 780
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 780
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Ser Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Ser Asn
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 781
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 781
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgt cgtgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctacagtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcaatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcgggt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcaatatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga aaatacgaat aacacttcac tgaaggacct cctg 1194
<210> 782
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 782
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Ser Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Ser Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asn Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Asn
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Asn Asn Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 783
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 783
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgt cgtgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgcatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctacagtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcaatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcaatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcaatt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgctttatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 784
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 784
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Ser Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Ser Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asn Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Asn Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 785
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 785
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgt cgtgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctacagtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcaatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcaatt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcaatatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgaa taatacgact aacacttcac tgaaggacct cctg 1194
<210> 786
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 786
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Ser Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Ser Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Asn Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Asn
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Asn Asn Thr Thr Asn Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 787
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 787
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgt cgtgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctacagtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcaatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga aaatacgact aacacttcac tgaaggacct cctg 1194
<210> 788
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 788
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Ser Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Ser Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Thr Asn Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 789
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 789
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgt cgtgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctggctatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcgggt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgctttatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga aaatacgatg caaacttcac tgaaggacct cctg 1194
<210> 790
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 790
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Ser Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Ala Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 791
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 791
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgt cgtgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcaatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcaatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 792
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 792
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Ser Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asn Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 793
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 793
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgt cgtgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcaatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc aacatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcgggt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgctttatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 794
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 794
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Ser Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asn Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Asn Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 795
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 795
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgt cgtgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcaatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcaatt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcaatatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 796
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 796
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Ser Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asn Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Asn Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Asn
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 797
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 797
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgt cgtgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcaatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgaa taatacgatg aacacttcac tgaaggacct cctg 1194
<210> 798
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 798
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Ser Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Asn Asn Thr Met Asn Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 799
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 799
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgt cgtgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcaatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcgggt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcaatatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgaa taatacgaat aacacttcac tgaaggacct cctg 1194
<210> 800
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 800
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Ser Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Asn
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Asn Asn Thr Asn Asn Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 801
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 801
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag cggtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 802
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 802
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Ala Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 803
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 803
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag aggtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 804
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 804
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Glu Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 805
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 805
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag gtgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 806
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 806
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Gly Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 807
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 807
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaac ttgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 808
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 808
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Leu Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 809
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 809
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaaa tggtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 810
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 810
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Met Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 811
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 811
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaaa gggtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 812
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 812
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Arg Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 813
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 813
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaat cggtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 814
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 814
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Ser Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 815
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 815
ctgagtaatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 816
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 816
Leu Ser Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 817
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 817
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggca ctaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 818
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 818
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Thr
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 819
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 819
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg tgaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 820
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 820
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Val
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 821
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 821
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggct ggaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 822
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 822
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Trp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 823
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 823
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcct taacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 824
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 824
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Leu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 825
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 825
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcag taacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 826
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 826
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Ser Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 827
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 827
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacgc tgctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 828
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 828
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Ala Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 829
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 829
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga tgctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 830
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 830
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Asp Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 831
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 831
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacct ggctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 832
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 832
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Leu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 833
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 833
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacat ggctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 834
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 834
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Met Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 835
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 835
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg attgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgcatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcaatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcgggt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgaa taatacgatg aacacttcac tgaaggacct cctg 1194
<210> 836
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 836
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Asn Asn Thr Met Asn Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 837
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 837
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgagcag 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 838
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 838
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Gln Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 839
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 839
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg agcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 840
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 840
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Glu Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 841
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 841
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaagctg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 842
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 842
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Ala Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 843
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 843
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaatgtg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 844
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 844
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Cys Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 845
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 845
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaatggg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 846
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 846
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Trp Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 847
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 847
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg attgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcaatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcaatt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcaatatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 848
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 848
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Asn Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Asn
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 849
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 849
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcaatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcaatt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga aaatacgaat aacacttcac tgaaggacct cctg 1194
<210> 850
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 850
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Asn Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Asn Asn Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 851
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 851
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg attgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcaatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcaatt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgctttatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga aaatacgagt aacacttcac tgaaggacct cctg 1194
<210> 852
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 852
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Asn Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Ser Asn Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 853
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 853
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg attgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgcatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcaatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcaatt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgctttatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga aaatacgatg aacacttcac tgaaggacct cctg 1194
<210> 854
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 854
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Asn Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Asn Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 855
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 855
ctggataatt tgttggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 856
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 856
Leu Asp Asn Leu Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 857
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 857
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgaggcta gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 858
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 858
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Ala Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 859
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 859
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca aggctatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 860
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 860
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys Ala Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 861
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 861
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca aggatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 862
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 862
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys Asp Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 863
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 863
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agtttatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 864
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 864
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys Phe Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 865
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 865
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agttgatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 866
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 866
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys Leu Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 867
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 867
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcgtatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 868
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 868
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys Arg Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 869
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 869
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agactatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 870
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 870
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys Thr Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 871
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 871
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat ccttgatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 872
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 872
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser Leu Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 873
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 873
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccagatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 874
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 874
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser Gln Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 875
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 875
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctg ggggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 876
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 876
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro Gly Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 877
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 877
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttcccta agggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 878
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 878
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro Lys Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 879
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 879
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc ggggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 880
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 880
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro Arg Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 881
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 881
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgaccagg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 882
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 882
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Gln Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 883
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 883
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacaggg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 884
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 884
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Arg Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 885
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 885
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgactcgg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 886
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 886
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ser Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 887
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 887
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacacgg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 888
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 888
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Thr Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 889
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 889
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacatttcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 890
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 890
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Phe
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 891
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 891
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaggtcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 892
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 892
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Gly
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 893
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 893
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaagtcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 894
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 894
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ser
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 895
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 895
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaacgcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 896
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 896
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Thr
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 897
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 897
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaca ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 898
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 898
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Gln Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 899
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 899
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcagt ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 900
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 900
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Val Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 901
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 901
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagaccttg atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 902
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 902
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Leu Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 903
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 903
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagaccctg atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 904
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 904
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Pro Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 905
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 905
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacactg atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 906
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 906
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Thr Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 907
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 907
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacgtgg atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 908
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 908
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Val Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 909
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 909
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggccttg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 910
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 910
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Leu Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 911
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 911
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggccggg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 912
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 912
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Arg Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 913
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 913
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcagtg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 914
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 914
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Ser Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 915
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 915
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggctggg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 916
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 916
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Trp Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 917
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 917
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgcat 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 918
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 918
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val His Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 919
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 919
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtggtt 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 920
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 920
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Val Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 921
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 921
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agacatctgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 922
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 922
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg His Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 923
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 923
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgggt 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 924
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 924
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Gly Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 925
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 925
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagttt 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgttctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 926
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 926
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Phe Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 927
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 927
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
tttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 928
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 928
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Phe Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 929
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 929
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
atgggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 930
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 930
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Met Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 931
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 931
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cttt 1194
<210> 932
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 932
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Phe
385 390 395
<210> 933
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 933
ctggataatg gaatggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 934
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 934
Leu Asp Asn Gly Met Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 935
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 935
ctggataatg gagtggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 936
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 936
Leu Asp Asn Gly Val Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 937
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 937
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg attgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgcatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcaatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga aaatacgagt caaacttcac tgaaggacct cctg 1194
<210> 938
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 938
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Ser Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 939
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 939
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcatacg ccagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggactagtcg gtaccaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
cgtaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 940
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 940
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Tyr Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 941
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 941
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggac gtcttcaggc tgatccgcag 240
cgcttccctc atggcatacg ccagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggactagtcg gtaccaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgttctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cttt 1194
<210> 942
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 942
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Tyr Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Phe
385 390 395
<210> 943
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 943
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggac gtcttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggactagtcg gtaccaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
accaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 944
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 944
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Tyr Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Thr Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 945
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 945
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggac gtcttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggactagtcg gtaccaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
accaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cttt 1194
<210> 946
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 946
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Tyr Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Thr Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Phe
385 390 395
<210> 947
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 947
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcca gcctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 948
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 948
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Gln Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 949
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 949
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcatacg ccagctggca agccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggagtagtcg gaaccaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
accaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgttctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 950
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 950
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Ser His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Ser Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Thr Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 951
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 951
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggcatttt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 952
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 952
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp His Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 953
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 953
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggctgttt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 954
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 954
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Leu Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 955
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 955
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggaggttt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 956
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 956
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Arg Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 957
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 957
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggtggttt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 958
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 958
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Trp Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 959
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 959
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt atgcgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 960
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 960
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Met Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 961
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 961
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccggc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 962
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 962
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Arg His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 963
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 963
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttctctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 964
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 964
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Ser His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 965
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 965
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cagcggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 966
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 966
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Ala Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 967
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 967
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac caggtgcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 968
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 968
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gly Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 969
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 969
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
attgacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 970
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 970
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Ile Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 971
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 971
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
atggacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 972
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 972
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Met Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 973
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 973
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgaggctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 974
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 974
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Arg Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 975
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 975
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttgcggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 976
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 976
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Ala Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 977
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 977
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gactttttgc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 978
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 978
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Phe Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 979
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 979
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttatggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 980
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 980
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Met Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 981
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 981
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gactttcggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 982
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 982
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Ser Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 983
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 983
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgacg 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 984
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 984
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Thr
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 985
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 985
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtattccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 986
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 986
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Ile Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 987
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 987
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atgcgcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 988
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 988
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Ala Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 989
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 989
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccagggggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 990
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 990
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Gly Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 991
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 991
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacatggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 992
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 992
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro His Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 993
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 993
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
accaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 994
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 994
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Thr Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 995
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 995
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
gttaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 996
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 996
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Val Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 997
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 997
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc gggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 998
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 998
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Gly Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 999
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 999
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctcttgt cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1000
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1000
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Leu Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1001
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1001
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctctctt cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1002
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1002
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Ser Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1003
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1003
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagatt atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1004
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1004
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ile Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1005
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1005
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatccttc cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1006
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1006
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Leu Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1007
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1007
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac ttttgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1008
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1008
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Phe Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1009
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1009
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac cattgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1010
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1010
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp His Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1011
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1011
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac ctgtgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1012
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1012
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Leu Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1013
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1013
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac acgtgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1014
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1014
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Thr Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1015
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1015
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac tggtgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1016
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1016
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Trp Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1017
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1017
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcacat 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1018
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1018
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala His Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1019
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1019
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaatt 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1020
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1020
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ile Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1021
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1021
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1022
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1022
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1023
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1023
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaatg 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1024
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1024
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Met Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1025
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1025
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcacct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1026
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1026
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Pro Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1027
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1027
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatgg 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1028
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1028
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Trp Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1029
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1029
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacgagga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1030
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1030
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Glu Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1031
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1031
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actactttga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1032
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1032
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Phe Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1033
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1033
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacatgga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1034
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1034
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Met Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1035
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1035
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actaccctga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1036
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1036
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Pro Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1037
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1037
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacaggga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1038
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1038
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Arg Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1039
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1039
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actactcgga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1040
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1040
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Ser Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1041
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1041
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actactatga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1042
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1042
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Tyr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1043
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1043
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggagtagtcg gtaccaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1044
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1044
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Ser Ser Arg Tyr Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1045
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1045
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atgcgattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1046
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1046
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Ala Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1047
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1047
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atctgattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1048
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1048
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Leu Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1049
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1049
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctgatagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1050
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1050
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Asp Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1051
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1051
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tcttgcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1052
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1052
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Leu Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1053
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1053
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccgtttaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1054
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1054
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Phe Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1055
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1055
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccgctgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1056
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1056
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Leu Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1057
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1057
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccgcgtaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1058
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1058
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Arg Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1059
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1059
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accattctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1060
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1060
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Ile Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1061
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1061
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accattctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cttt 1194
<210> 1062
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1062
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Ile Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Phe
385 390 395
<210> 1063
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1063
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttatga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1064
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1064
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Tyr Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1065
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1065
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt gtacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1066
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1066
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Cys Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1067
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1067
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtg tgacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1068
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1068
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Val Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1069
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1069
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catggtgttc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1070
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1070
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Cys Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1071
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1071
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggattc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1072
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1072
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Asp Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1073
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1073
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggaatc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1074
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1074
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Glu Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1075
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1075
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catggttttc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1076
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1076
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Phe Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1077
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1077
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggggtc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1078
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1078
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Gly Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1079
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1079
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catggcattc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1080
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1080
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp His Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1081
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1081
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catggatctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1082
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1082
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ile Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1083
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1083
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catggaaatc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1084
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1084
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1085
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1085
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catggctgtc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1086
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1086
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Leu Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1087
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1087
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catggatgtc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1088
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1088
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Met Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1089
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1089
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catggaattc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1090
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1090
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Asn Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1091
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1091
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catggccatc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1092
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1092
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Pro Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1093
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1093
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catggcaatc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1094
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1094
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Gln Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1095
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1095
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catggcgttc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1096
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1096
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Arg Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1097
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1097
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catggagttc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1098
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1098
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ser Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1099
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1099
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catggacttc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1100
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1100
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Thr Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1101
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1101
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggtctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1102
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1102
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Val Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1103
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1103
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catggtggtc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1104
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1104
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Trp Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1105
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1105
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catggtattc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1106
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1106
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Tyr Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1107
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1107
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc tgtatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1108
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1108
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Cys Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1109
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1109
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gatatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1110
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1110
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Asp Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1111
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1111
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gagatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1112
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1112
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Glu Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1113
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1113
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc tttatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1114
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1114
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Phe Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1115
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1115
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc ggtatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1116
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1116
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Gly Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1117
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1117
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc cacatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1118
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1118
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1119
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1119
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc attatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1120
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1120
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ile Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1121
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1121
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc aagatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1122
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1122
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Lys Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1123
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1123
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc ttgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1124
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1124
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Leu Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1125
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1125
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc atgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1126
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1126
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Met Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1127
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1127
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc aatatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1128
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1128
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Asn Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1129
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1129
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc cctatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1130
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1130
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Pro Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1131
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1131
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc cagatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1132
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1132
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Gln Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1133
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1133
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc cggatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1134
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1134
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Arg Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1135
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1135
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc tcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1136
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1136
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ser Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1137
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1137
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc actatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1138
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1138
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Thr Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1139
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1139
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gttatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1140
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1140
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Val Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1141
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1141
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc tggatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1142
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1142
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Trp Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1143
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1143
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc tacatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1144
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1144
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Tyr Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1145
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1145
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga atgtaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1146
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1146
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Cys
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1147
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1147
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agataccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1148
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1148
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Asp
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1149
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1149
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agaaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1150
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1150
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Glu
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1151
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1151
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga atttaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1152
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1152
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Phe
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1153
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1153
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga aggtaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1154
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1154
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Gly
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1155
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1155
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga acataccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1156
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1156
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu His
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1157
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1157
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga aattaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1158
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1158
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ile
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1159
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1159
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga aaagaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1160
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1160
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Lys
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1161
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1161
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga acttaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1162
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1162
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Leu
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1163
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1163
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga aatgaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1164
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1164
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Met
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1165
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1165
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga aaataccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1166
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1166
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Asn
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1167
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1167
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga acctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1168
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1168
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Pro
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1169
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1169
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga acaaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1170
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1170
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Gln
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1171
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1171
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga acggaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1172
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1172
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Arg
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1173
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1173
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga aagtaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1174
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1174
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ser
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1175
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1175
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga aacgaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1176
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1176
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Thr
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1177
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1177
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agtgaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1178
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1178
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Val
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1179
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1179
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga atggaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1180
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1180
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1181
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1181
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga atataccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1182
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1182
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Tyr
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1183
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1183
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttgggc tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1184
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1184
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Ala Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1185
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1185
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggtg tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1186
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1186
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Cys Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1187
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1187
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga gcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1188
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1188
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Glu Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1189
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1189
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggtt tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1190
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1190
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Phe Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1191
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1191
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttgggg gcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1192
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1192
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Gly Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1193
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1193
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggca tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1194
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1194
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp His Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1195
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1195
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggat tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1196
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1196
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Ile Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1197
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1197
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggaa gcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1198
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1198
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Lys Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1199
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1199
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggtt gcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1200
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1200
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Leu Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1201
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1201
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggat gcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1202
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1202
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Met Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1203
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1203
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggaa tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1204
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1204
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1205
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1205
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggcc gcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1206
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1206
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Pro Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1207
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1207
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggca gcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1208
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1208
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Gln Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1209
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1209
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggcg gcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1210
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1210
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Arg Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1211
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1211
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggtc tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1212
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1212
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Ser Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1213
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1213
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggac gcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1214
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1214
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Thr Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1215
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1215
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttgggt gcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1216
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1216
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Val Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1217
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1217
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggtg gcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1218
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1218
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Trp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1219
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1219
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggta tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1220
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1220
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Tyr Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1221
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1221
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagcggc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1222
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1222
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Ala Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1223
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1223
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggatgtgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1224
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1224
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Cys Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1225
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1225
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagaggc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1226
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1226
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Glu Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1227
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1227
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggatttgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1228
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1228
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Phe Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1229
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1229
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggaggggc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1230
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1230
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Gly Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1231
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1231
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggacatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1232
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1232
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly His Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1233
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1233
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggaattgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1234
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1234
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Ile Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1235
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1235
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggaaaagc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1236
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1236
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Lys Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1237
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1237
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggacttgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1238
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1238
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1239
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1239
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggaatggc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1240
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1240
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Met Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1241
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1241
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggaaacgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1242
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1242
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asn Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1243
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1243
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggacctgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1244
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1244
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Pro Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1245
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1245
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggacaggc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1246
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1246
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Gln Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1247
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1247
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggacgggc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1248
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1248
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Arg Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1249
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1249
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggatcggc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1250
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1250
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Ser Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1251
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1251
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggaacggc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1252
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1252
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Thr Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1253
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1253
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagtggc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1254
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1254
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Val Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1255
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1255
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggatgggc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1256
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1256
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Trp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1257
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1257
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggatatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1258
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1258
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Tyr Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1259
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1259
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgcggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1260
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1260
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Ala Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1261
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1261
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct cttttgtgaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1262
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1262
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Cys Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1263
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1263
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgatgaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacctcac tgaaggacct cctg 1194
<210> 1264
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1264
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Asp Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1265
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1265
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttttcgaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1266
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1266
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Phe Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1267
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1267
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttggtgaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1268
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1268
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Gly Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1269
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1269
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttcatgaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1270
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1270
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe His Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1271
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1271
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttattgaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1272
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1272
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Ile Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1273
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1273
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttaaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1274
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1274
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Lys Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1275
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1275
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttctggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1276
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1276
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Leu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1277
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1277
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttatggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1278
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1278
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1279
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1279
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttaatgaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1280
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1280
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Asn Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1281
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1281
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttccggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1282
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1282
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Pro Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1283
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1283
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttcaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1284
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1284
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Gln Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1285
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1285
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttagggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1286
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1286
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Arg Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1287
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1287
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttagcgaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1288
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1288
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Ser Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1289
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1289
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttacggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1290
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1290
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Thr Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1291
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1291
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgtggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1292
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1292
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Val Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1293
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1293
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct cttttgggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1294
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1294
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Trp Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1295
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1295
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct cttttatgaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1296
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1296
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Tyr Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1297
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1297
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
gcgcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1298
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1298
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1299
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1299
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
tgtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1300
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1300
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Cys Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1301
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1301
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
gatcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1302
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1302
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Asp Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1303
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1303
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
gagcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1304
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1304
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Glu Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1305
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1305
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
tttcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1306
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1306
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Phe Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1307
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1307
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
caccctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1308
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1308
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala His Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1309
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1309
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
attcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1310
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1310
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Ile Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1311
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1311
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
aagcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1312
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1312
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Lys Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1313
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1313
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ttgcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1314
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1314
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Leu Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1315
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1315
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
atgcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1316
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1316
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Met Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1317
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1317
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
aatcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1318
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1318
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Asn Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1319
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1319
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ccgcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1320
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1320
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Pro Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1321
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1321
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
cagcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1322
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1322
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gln Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1323
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1323
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
aggcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1324
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1324
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Arg Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1325
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1325
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
tcgcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1326
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1326
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Ser Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1327
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1327
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
acgcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1328
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1328
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Thr Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1329
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1329
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
gtgcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1330
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1330
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Val Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1331
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1331
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
tggcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1332
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1332
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Trp Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1333
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1333
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
tatcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1334
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1334
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Tyr Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1335
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1335
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aacgctgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1336
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1336
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Ala Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1337
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1337
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactgtgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1338
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1338
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Cys Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1339
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1339
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aacgatgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1340
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1340
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Asp Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1341
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1341
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aacgaggtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1342
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1342
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Glu Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1343
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1343
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactttgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1344
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1344
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Phe Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1345
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1345
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aacggtgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1346
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1346
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Gly Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1347
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1347
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aacatcgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1348
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1348
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Ile Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1349
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1349
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aacaaggtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1350
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1350
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Lys Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1351
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1351
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aacttggtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1352
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1352
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Leu Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1353
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1353
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aacatggtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1354
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1354
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Met Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1355
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1355
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aacaatgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1356
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1356
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Asn Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1357
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1357
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aacccggtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1358
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1358
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Pro Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1359
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1359
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccaggtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1360
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1360
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Gln Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1361
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1361
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccgtgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1362
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1362
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Arg Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1363
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1363
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactcggtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1364
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1364
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Ser Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1365
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1365
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aacactgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1366
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1366
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Thr Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1367
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1367
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aacgttgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1368
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1368
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Val Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1369
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1369
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactgggtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1370
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1370
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Trp Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1371
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1371
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1372
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1372
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1373
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1373
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccgcgatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1374
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1374
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ala Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1375
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1375
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gcctgtatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1376
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1376
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Cys Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1377
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1377
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccgatatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1378
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1378
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Asp Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1379
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1379
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccgagatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1380
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1380
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Glu Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1381
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1381
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gcctttatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1382
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1382
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Phe Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1383
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1383
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccggtatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1384
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1384
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Gly Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1385
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1385
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gcccatatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1386
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1386
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala His Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1387
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1387
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccaagatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1388
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1388
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Lys Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1389
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1389
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gcccttatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1390
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1390
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Leu Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1391
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1391
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccatgatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1392
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1392
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1393
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1393
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccaatatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1394
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1394
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Asn Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1395
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1395
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gcccctatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1396
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1396
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Pro Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1397
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1397
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gcccagatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1398
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1398
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Gln Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1399
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1399
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gcccggatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1400
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1400
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Arg Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1401
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1401
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gcctctatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1402
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1402
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ser Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1403
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1403
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccacgatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1404
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1404
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Thr Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1405
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1405
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccgttatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1406
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1406
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Val Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1407
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1407
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gcctggatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1408
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1408
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Trp Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1409
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1409
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gcctatatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1410
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1410
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Tyr Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1411
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1411
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agagcgggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1412
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1412
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Ala Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1413
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1413
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agatgcggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1414
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1414
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Cys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1415
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1415
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agagatggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1416
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1416
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Asp Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1417
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1417
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agagagggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1418
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1418
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Glu Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1419
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1419
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agatttggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1420
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1420
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Phe Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1421
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1421
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaggtggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1422
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1422
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gly Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1423
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1423
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agacatggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1424
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1424
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg His Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1425
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1425
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaattggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1426
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1426
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Ile Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1427
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1427
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agactgggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1428
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1428
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Leu Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1429
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1429
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaatgggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1430
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1430
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Met Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1431
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1431
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaatggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1432
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1432
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Asn Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1433
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1433
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaccgggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1434
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1434
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Pro Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1435
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1435
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agacagggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1436
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1436
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1437
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1437
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaggggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1438
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1438
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Arg Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1439
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1439
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agatctggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1440
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1440
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Ser Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1441
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1441
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaactggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1442
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1442
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Thr Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1443
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1443
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agagtgggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1444
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1444
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Val Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1445
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1445
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agatggggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1446
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1446
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Trp Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1447
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1447
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agatatggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1448
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1448
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Tyr Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1449
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1449
ctggataatg gattggctag aacacctgct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1450
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1450
Leu Asp Asn Gly Leu Ala Arg Thr Pro Ala Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1451
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1451
ctggataatg gattggctag aacaccttgt atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1452
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1452
Leu Asp Asn Gly Leu Ala Arg Thr Pro Cys Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1453
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1453
ctggataatg gattggctag aacacctgat atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcatc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1454
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1454
Leu Asp Asn Gly Leu Ala Arg Thr Pro Asp Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1455
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1455
ctggataatg gattggctag aacacctgag atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1456
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1456
Leu Asp Asn Gly Leu Ala Arg Thr Pro Glu Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1457
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1457
ctggataatg gattggctag aacacctttt atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1458
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1458
Leu Asp Asn Gly Leu Ala Arg Thr Pro Phe Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1459
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1459
ctggataatg gattggctag aacacctggc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agccaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1460
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1460
Leu Asp Asn Gly Leu Ala Arg Thr Pro Gly Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1461
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1461
ctggataatg gattggctag aacacctcat atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1462
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1462
Leu Asp Asn Gly Leu Ala Arg Thr Pro His Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1463
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1463
ctggataatg gattggctag aacacctatt atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1464
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1464
Leu Asp Asn Gly Leu Ala Arg Thr Pro Ile Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1465
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1465
ctggataatg gattggctag aacacctaag atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1466
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1466
Leu Asp Asn Gly Leu Ala Arg Thr Pro Lys Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1467
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1467
ctggataatg gattggctag aacacctttg atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1468
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1468
Leu Asp Asn Gly Leu Ala Arg Thr Pro Leu Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1469
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1469
ctggataatg gattggctag aacacctatg atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1470
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1470
Leu Asp Asn Gly Leu Ala Arg Thr Pro Met Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1471
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1471
ctggataatg gattggctag aacacctaat atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1472
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1472
Leu Asp Asn Gly Leu Ala Arg Thr Pro Asn Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1473
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1473
ctggataatg gattggctag aacacctcag atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1474
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1474
Leu Asp Asn Gly Leu Ala Arg Thr Pro Gln Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1475
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1475
ctggataatg gattggctag aacacctcgt atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1476
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1476
Leu Asp Asn Gly Leu Ala Arg Thr Pro Arg Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1477
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1477
ctggataatg gattggctag aacaccttct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1478
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1478
Leu Asp Asn Gly Leu Ala Arg Thr Pro Ser Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1479
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1479
ctggataatg gattggctag aacacctact atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1480
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1480
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1481
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1481
ctggataatg gattggctag aacacctgtc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1482
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1482
Leu Asp Asn Gly Leu Ala Arg Thr Pro Val Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1483
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1483
ctggataatg gattggctag aacaccttgg atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1484
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1484
Leu Asp Asn Gly Leu Ala Arg Thr Pro Trp Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1485
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1485
ctggataatg gattggctag aacaccttat atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1486
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1486
Leu Asp Asn Gly Leu Ala Arg Thr Pro Tyr Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1487
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1487
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcgggcgat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1488
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1488
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Ala Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1489
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1489
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtgcat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1490
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1490
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Cys Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1491
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1491
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcgggatat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1492
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1492
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Asp Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1493
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1493
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcgggagat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1494
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1494
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Glu Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1495
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1495
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtttat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1496
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1496
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Phe Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1497
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1497
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcgggggat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1498
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1498
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Gly Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1499
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1499
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggcatat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1500
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1500
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg His Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1501
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1501
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggattat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1502
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1502
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Ile Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1503
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1503
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggaagat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1504
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1504
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Lys Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1505
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1505
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggctgat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1506
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1506
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Leu Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1507
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1507
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggatgat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1508
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1508
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Met Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1509
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1509
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggaacat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1510
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1510
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Asn Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1511
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1511
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggcagat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1512
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1512
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Gln Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1513
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1513
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggcggat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1514
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1514
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Arg Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1515
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1515
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggagtat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1516
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1516
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1517
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1517
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggaccat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1518
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1518
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Thr Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1519
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1519
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcgggtgat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1520
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1520
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Val Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1521
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1521
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtggat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1522
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1522
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Trp Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1523
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1523
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtatat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1524
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1524
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Tyr Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1525
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1525
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattgc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1526
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1526
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1527
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1527
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaatttg tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1528
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1528
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Cys Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1529
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1529
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattga tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1530
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1530
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Asp Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1531
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1531
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattga ggtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1532
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1532
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Glu Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1533
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1533
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaatttt tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1534
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1534
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Phe Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1535
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1535
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattgg ggtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1536
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1536
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Gly Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1537
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1537
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattca cgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1538
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1538
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
His Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1539
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1539
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattat tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1540
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1540
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Ile Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1541
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1541
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattaa ggtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1542
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1542
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Lys Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1543
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1543
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaatttt ggtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1544
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1544
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Leu Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1545
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1545
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattat ggtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1546
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1546
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Met Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1547
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1547
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattaa tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1548
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1548
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Asn Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1549
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1549
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattca ggtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1550
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1550
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Gln Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1551
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1551
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattag ggtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1552
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1552
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Arg Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1553
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1553
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattag tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1554
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1554
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Ser Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1555
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1555
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattac ggtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1556
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1556
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Thr Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1557
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1557
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattgt ggtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1558
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1558
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Val Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1559
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1559
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaatttg ggtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1560
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1560
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Trp Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1561
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1561
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattta tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1562
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1562
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Tyr Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1563
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1563
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtgc gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1564
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1564
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Ala Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1565
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1565
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagttg caatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1566
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1566
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Cys Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1567
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1567
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtga taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1568
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1568
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Asp Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1569
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1569
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtga gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1570
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1570
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Glu Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1571
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1571
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagttt taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1572
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1572
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1573
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1573
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtgg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1574
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1574
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Gly Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1575
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1575
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtca taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1576
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1576
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser His Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1577
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1577
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtat taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1578
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1578
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Ile Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1579
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1579
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtaa gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1580
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1580
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Lys Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1581
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1581
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtct gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1582
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1582
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Leu Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1583
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1583
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtat gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1584
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1584
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Met Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1585
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1585
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtaa taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1586
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1586
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Asn Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1587
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1587
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcc taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1588
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1588
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Pro Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1589
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1589
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtca gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1590
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1590
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Gln Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1591
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1591
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtag taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1592
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1592
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Ser Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1593
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1593
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtac taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1594
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1594
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Thr Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1595
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1595
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtgt taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1596
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1596
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Val Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1597
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1597
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagttg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1598
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1598
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Trp Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1599
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1599
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctaga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtta caatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1600
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1600
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Tyr Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1601
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1601
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaag ctatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1602
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1602
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Ala Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1603
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1603
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaat gtatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1604
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1604
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Cys Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1605
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1605
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaag atatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1606
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1606
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Asp Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1607
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1607
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaag agatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1608
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1608
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Glu Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1609
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1609
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaat ttatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1610
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1610
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Phe Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1611
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1611
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaag gtatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1612
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1612
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Gly Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1613
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1613
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac atatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1614
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1614
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu His Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1615
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1615
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaaa ttatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1616
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1616
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Ile Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1617
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1617
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaaa agatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1618
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1618
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Lys Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1619
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1619
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac tgatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1620
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1620
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1621
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1621
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaaa atatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1622
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1622
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Asn Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1623
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1623
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac cgatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1624
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1624
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Pro Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1625
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1625
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac agatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1626
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1626
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Gln Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1627
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1627
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaaa gtatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1628
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1628
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Ser Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1629
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1629
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaaa ccatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1630
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1630
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Thr Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1631
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1631
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaag ttatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1632
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1632
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Val Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1633
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1633
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaat ggatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1634
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1634
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Trp Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1635
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1635
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaat atatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1636
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1636
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Tyr Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1637
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1637
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg caggcgtcac tgaaggacct cctg 1194
<210> 1638
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1638
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Ala Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1639
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1639
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagtgttcac tgaaggacct cctg 1194
<210> 1640
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1640
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Cys Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1641
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1641
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg caggactcac tgaaggacct cctg 1194
<210> 1642
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1642
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Asp Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1643
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1643
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg caggagtcac tgaaggacct cctg 1194
<210> 1644
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1644
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Glu Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1645
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1645
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagttttcac tgaaggacct cctg 1194
<210> 1646
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1646
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Phe Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1647
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1647
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagggttcac tgaaggacct cctg 1194
<210> 1648
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1648
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Gly Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1649
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1649
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagcattcac tgaaggacct cctg 1194
<210> 1650
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1650
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln His Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1651
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1651
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagatttcac tgaaggacct cctg 1194
<210> 1652
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1652
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Ile Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1653
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1653
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagaagtcac tgaaggacct cctg 1194
<210> 1654
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1654
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Lys Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1655
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1655
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagctgtcac tgaaggacct cctg 1194
<210> 1656
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1656
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Leu Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1657
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1657
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagatgtcac tgaaggacct cctg 1194
<210> 1658
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1658
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1659
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1659
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagaattcac tgaaggacct cctg 1194
<210> 1660
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1660
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Asn Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1661
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1661
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagccttcac tgaaggacct cctg 1194
<210> 1662
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1662
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Pro Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1663
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1663
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagcagtcac tgaaggacct cctg 1194
<210> 1664
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1664
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Gln Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1665
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1665
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagcgttcac tgaaggacct cctg 1194
<210> 1666
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1666
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Arg Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1667
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1667
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagtcttcac tgaaggacct cctg 1194
<210> 1668
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1668
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Ser Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1669
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1669
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg caggtgtcac tgaaggacct cctg 1194
<210> 1670
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1670
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Val Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1671
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1671
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagtggtcac tgaaggacct cctg 1194
<210> 1672
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1672
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Trp Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1673
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1673
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagtattcac tgaaggacct cctg 1194
<210> 1674
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1674
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Tyr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1675
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1675
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtggc tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1676
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1676
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Ala Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1677
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1677
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgtg tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1678
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1678
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Cys Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1679
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1679
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgga tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1680
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1680
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Asp Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1681
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1681
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgga ggagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1682
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1682
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Glu Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1683
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1683
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgtt tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1684
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1684
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Phe Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1685
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1685
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtggg ggagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1686
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1686
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Gly Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1687
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1687
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgca cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1688
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1688
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val His Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1689
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1689
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgat tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1690
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1690
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Ile Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1691
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1691
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgaa ggagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1692
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1692
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Lys Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1693
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1693
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgtt ggagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1694
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1694
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Leu Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1695
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1695
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgat ggagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1696
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1696
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Met Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1697
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1697
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgaa tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1698
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1698
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Asn Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1699
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1699
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgcc ggagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1700
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1700
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Pro Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1701
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1701
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgca ggagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1702
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1702
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Gln Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1703
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1703
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgag ggagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1704
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1704
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Arg Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1705
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1705
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgtc tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1706
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1706
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Ser Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1707
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1707
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtggt ggagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1708
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1708
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Val Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1709
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1709
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgtg ggagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1710
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1710
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Trp Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1711
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1711
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgta tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1712
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1712
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Tyr Glu
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile
325 330 335
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1713
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1713
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaaa gtgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1714
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1714
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Ser Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1715
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1715
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aacaccgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agaaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1716
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1716
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Thr Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Glu
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1717
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1717
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacacgg acgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1718
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1718
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Thr Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1719
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1719
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggcctat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcttc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacacgg aagtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1720
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1720
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Thr Glu Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1721
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1721
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaaa tggtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt acacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1722
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1722
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Met Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1723
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1723
ctggataatg gattggctag aacacctcca atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aacaccgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcatc cataaagagc atcttggact ggaccagtcg taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1724
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1724
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Thr Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1725
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1725
ctggataatg gattggctag aacacctcca atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccaggtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt acacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agcaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1726
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1726
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Gln Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1727
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1727
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac acctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gtatggtgcg cgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggcctat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1728
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1728
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Thr Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Arg Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1729
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1729
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgtggaa 120
atggccgaag tgatggtgcg cgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
agccctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacctgg ccgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1730
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1730
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Val Glu Met Ala Glu Val Met Val Arg Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Ser Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Leu Ala Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1731
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1731
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttctggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaaa gtgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agcaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1732
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1732
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Leu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Ser Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1733
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1733
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catggatttc cataaagagc atcttggact ggaccagtcg taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag acgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1734
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1734
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ile Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1735
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1735
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgtggaa 120
atggccgaag tgatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggcctat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcttc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacacgg acgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1736
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1736
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Val Glu Met Ala Glu Val Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Thr Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1737
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1737
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaagaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aacgtagtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cagcagcaaa ggccctgctc 840
caagacaaaa gtgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agcaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1738
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1738
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Val Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Ala Ala Lys Ala Leu Leu Gln Asp Lys Ser Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1739
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1739
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aacaccgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gctatctcac cacaggcaaa ggccctgctc 840
caagaccctg atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt acacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1740
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1740
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Thr Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Pro Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1741
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1741
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aacaccgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaaa tggtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1742
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1742
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Thr Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Met Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1743
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1743
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agttcatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag acgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1744
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1744
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys Phe Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1745
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1745
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agactgggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1746
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1746
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Leu Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1747
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1747
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
agccctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1748
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1748
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Ser Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1749
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1749
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc aagatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agaaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1750
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1750
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Lys Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Glu
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1751
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1751
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttcccta aaggcataag gcagctggca aacgtagtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1752
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1752
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro Lys Gly Ile Arg Gln Leu Ala Asn Val Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1753
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1753
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aacaccgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcatc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc aagatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1754
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1754
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Thr Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Lys Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1755
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1755
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aacgtagtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggaatc cataaagagc atcttggact ggaccagtcg taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaaa gtgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agcaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1756
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1756
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Val Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Glu Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Ser Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1757
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1757
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
agccctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacctgg acgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1758
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1758
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Ser Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Leu Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1759
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1759
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac acctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1760
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1760
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Thr Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1761
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1761
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttcccta aaggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaaa gtgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agactgggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg caggcgtcac tgaaggacct cctg 1194
<210> 1762
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1762
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro Lys Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Ser Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Leu Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Ala Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1763
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1763
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgtggaa 120
atggccgaag tgatggtgcg cgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1764
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1764
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Val Glu Met Ala Glu Val Met Val Arg Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1765
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1765
ctggataatg gattggctag aacacctcca atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttcccta aaggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agaaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg caggcgtcac tgaaggacct cctg 1194
<210> 1766
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1766
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro Lys Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Glu
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Ala Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1767
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1767
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggacatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1768
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1768
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly His Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1769
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1769
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac acctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggcctat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacctgg ccgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1770
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1770
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Thr Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Leu Ala Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1771
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1771
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc aagatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1772
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1772
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Lys Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1773
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1773
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agaaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagtggtcac tgaaggacct cctg 1194
<210> 1774
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1774
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Glu
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Trp Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1775
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1775
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaag tgatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggcctat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1776
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1776
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Val Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1777
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1777
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catggcaatc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc agtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1778
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1778
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Gln Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1779
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1779
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttctggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1780
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1780
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Leu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1781
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1781
ctggataatg gattggctag aacacctcca atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaagaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttcccta aaggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggacatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1782
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1782
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro Lys Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly His Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1783
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1783
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aacaccgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacgtcg atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagtggtcac tgaaggacct cctg 1194
<210> 1784
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1784
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Thr Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Val Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Trp Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1785
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1785
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gtatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggcctat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacctgg acgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1786
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1786
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Leu Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1787
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1787
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcgggatat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacctgg ccgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1788
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1788
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Asp Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Leu Ala Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1789
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1789
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac acctgcatct ccgagaagct ctttgtggaa 120
atggccgaag tgatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcgggatat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agatatggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1790
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1790
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Thr Cys
20 25 30
Ile Ser Glu Lys Leu Phe Val Glu Met Ala Glu Val Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Asp Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Tyr Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1791
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1791
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc agtaaacaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1792
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1792
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Asn Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1793
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1793
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gctatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt gcacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1794
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1794
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Cys Thr Ile
325 330 335
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1795
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1795
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttctggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aacgtagtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1796
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1796
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Leu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Val Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1797
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1797
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agttcatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag acgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agaaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1798
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1798
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys Phe Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Glu
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1799
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1799
ctggataatg gattggctag aacacctcca atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aacgtagtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1800
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1800
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Val Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1801
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1801
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aacgtagtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaaa gtgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1802
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1802
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Val Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Ser Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1803
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1803
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaagg aagtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1804
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1804
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Glu Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1805
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1805
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gctatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagtggtcac tgaaggacct cctg 1194
<210> 1806
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1806
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Trp Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1807
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1807
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aacgtagtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggacatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1808
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1808
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Val Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly His Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1809
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1809
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagaccctg atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagtggtcac tgaaggacct cctg 1194
<210> 1810
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1810
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Pro Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Trp Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1811
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1811
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catggacctc cataaagagc atcttggact ggaccagtcg taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt gcacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agcaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1812
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1812
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Thr Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Cys Thr Ile
325 330 335
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1813
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1813
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agaaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1814
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1814
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Glu
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1815
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1815
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgtggaa 120
atggccgaac gtatggtgcg cgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggcctat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
agccctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1816
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1816
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Val Glu Met Ala Glu Arg Met Val Arg Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Ser Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1817
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1817
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cagcagcaaa ggccctgctc 840
caagacaaag acgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1818
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1818
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Ala Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1819
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1819
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaagaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aacgtagtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catggtactc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cagcagcaaa ggccctgctc 840
caagacaaag acgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1820
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1820
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Val Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Tyr Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Ala Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1821
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1821
ctggataatg gattggctag aacacctggt atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1822
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1822
Leu Asp Asn Gly Leu Ala Arg Thr Pro Gly Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1823
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1823
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aacaccgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggaatc cataaagagc atcttggact ggaccagtaa caatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1824
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1824
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Thr Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Glu Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Asn Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1825
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1825
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg caggcgtcac tgaaggacct cctg 1194
<210> 1826
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1826
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Ala Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1827
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1827
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg caggactcac tgaaggacct cctg 1194
<210> 1828
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1828
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Asp Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1829
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1829
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgtggaa 120
atggccgaag tgatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggcctat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc agtaaacaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1830
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1830
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Val Glu Met Ala Glu Val Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Asn Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1831
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1831
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgtggaa 120
atggccgaag tgatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggcctat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1832
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1832
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Val Glu Met Ala Glu Val Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1833
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1833
ctggataatg gattggctag aacacctcca atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catggtactc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1834
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1834
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Tyr Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1835
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1835
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aacaccgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcatc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc aagatctcac cacaggcaag ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaagggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agcaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1836
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1836
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Thr Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Lys Ile
260 265 270
Ser Pro Gln Ala Arg Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1837
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1837
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aacaccgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggaatc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gctatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaacgggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt acacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agaaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1838
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1838
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Thr Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Glu Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Thr Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Glu
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1839
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1839
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggaatc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1840
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1840
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Glu Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1841
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1841
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttcccta aaggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1842
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1842
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro Lys Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1843
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1843
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcgggatat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1844
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1844
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Asp Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1845
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1845
ctggataatg gattggctag aacacctcca atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggaatc cataaagagc atcttggact ggaccagtaa caatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1846
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1846
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Glu Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Asn Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1847
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1847
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agttcatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtat taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1848
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1848
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys Phe Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Ile Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1849
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1849
ctggataatg gattggctag aacacctggt atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccatgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcatc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt acacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg caggactcac tgaaggacct cctg 1194
<210> 1850
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1850
Leu Asp Asn Gly Leu Ala Arg Thr Pro Gly Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Asp Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1851
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1851
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttctggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggaatc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1852
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1852
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Leu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Glu Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1853
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1853
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agatatggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1854
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1854
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Tyr Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1855
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1855
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aacgtagtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agactgggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1856
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1856
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Val Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Leu Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1857
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1857
ctggataatg gattggctag aacacctcca atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aacaccgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcatc cataaagagc atcttggact ggaccagtcg taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gctatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agactgggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1858
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1858
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Thr Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Leu Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1859
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1859
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcgggatat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agatatggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1860
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1860
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Asp Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Tyr Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1861
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1861
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaag tgatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcggcctat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaagg aagtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agatatggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1862
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1862
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Val Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Glu Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Tyr Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
<210> 1863
<211> 1194
<212> DNA
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1863
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgacggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aataaaaccg gtcgggatat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacacgg acgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgcggtt atacaattcc tgtagcaaag 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg 1194
<210> 1864
<211> 398
<212> PRT
<213> Artificial Sequence
<220>
<223> variant of human alpha-galactosidase A
<400> 1864
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp
1 5 10 15
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys
20 25 30
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp
35 40 45
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp
50 55 60
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln
65 70 75 80
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys
85 90 95
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala
100 105 110
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe
115 120 125
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp
130 135 140
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu
145 150 155 160
Asn Lys Thr Gly Arg Asp Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr
165 170 175
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys
180 185 190
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile
195 200 205
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp
210 215 220
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly
225 230 235 240
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp
245 250 255
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile
260 265 270
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Thr Asp Val Ile Ala Ile
275 280 285
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp
290 295 300
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val
305 310 315 320
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile
325 330 335
Pro Val Ala Lys Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe
340 345 350
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala
355 360 365
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu
370 375 380
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu
385 390 395
Claims (41)
- 서열번호 374, 704 및/또는 1022에 대해 적어도 70%, 적어도 75%, 적어도 80%, 적어도 85%, 적어도 90%, 적어도 91%, 적어도 92%, 적어도 93%, 적어도 94%, 적어도 95%, 적어도 96%, 적어도 97%, 적어도 98% 또는 적어도 99%의 서열 동일성을 포함하는 아미노산 서열을 포함하는, 재조합 알파-갈락토시다제 A 및/또는 생물학적 활성 재조합 알파-갈락토시다제 A 단편.
- 제1항에 있어서, 상기 재조합 알파-갈락토시다제는 서열번호 8에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 이상의 서열 동일성을 가지는 폴리펩타이드 서열 또는 이의 기능적 단편을 포함하고, 상기 재조합 알파-갈락토시다제 A는 2, 4, 5, 24/59, 24/143/144, 24/143/202/333, 24/143/202/352/390/391, 24/143/333/352/387/390/391, 24/143/390/391, 24/202, 24/202/271, 24/202/333/352, 24/271/352, 24/352/387/390/391, 24/387/391, 31, 40, 59, 59/143, 59/143/202, 59/143/202/271/333, 59/143/271, 59/143/333, 59/202, 59/202/333, 59/271/387/390, 73, 76, 80, 83, 84, 91/215/361, 122, 123, 143, 143/202, 143/271, 143/271/352/390, 143/333, 143/333/387/390, 143/387/391, 147, 155, 164, 165, 179, 186, 202, 202/333, 210, 215/218, 218, 218/361, 218/361/398, 218/398, 246, 254/398, 271, 271/333, 271/333/390/391, 271/333/391, 271/352/391, 273, 275, 277, 278, 280, 281, 283, 284, 287, 300, 303, 304, 325, 331, 332, 333/352, 333/390/391, 333/391, 334, 335, 336, 338, 339, 340, 341, 343, 359, 360, 361, 362, 367, 369, 371, 373, 375, 377, 382, 382/398, 385, 387/391, 390 및 398로부터 선택된 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하며, 상기 폴리펩타이드 서열의 아미노산 위치는 서열번호 704를 기준으로 넘버링되는 것인 재조합 알파-갈락토시다제 A.
- 제1항에 있어서, 상기 재조합 알파-갈락토시다제는 서열번호 8에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 이상의 서열 동일성을 가지는 폴리펩타이드 서열 또는 이의 기능적 단편을 포함하고, 상기 재조합 알파-갈락토시다제 A는 10, 39, 44, 47, 92, 166, 206, 217, 247, 261, 271, 302, 316, 322, 337, 368 및 392로부터 선택된 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하며, 상기 폴리펩타이드 서열의 아미노산 위치는 서열번호 374를 기준으로 넘버링되는 것인 재조합 알파-갈락토시다제 A.
- 제1항에 있어서, 상기 재조합 알파-갈락토시다제는 서열번호 58에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 이상의 서열 동일성을 가지는 폴리펩타이드 서열 또는 이의 기능적 단편을 포함하고, 상기 재조합 알파-갈락토시다제 A는 10, 10/392, 31, 31/39/44/166/302, 31/47, 31/283/284, 39, 39/44, 39/44/47, 39/44/47/261/283/284, 39/44/283, 39/44/339, 39/47/261, 39/92, 39/206, 39/284, 44, 44/284/302, 84, 84/92, 84/284/302/392, 84/316, 84/368/392, 92, 92/206/217, 92/206/275, 92/206/284, 92/206/302/368, 92/271, 92/271/277, 92/275/284, 92/283, 92/283/392, 92/284, 92/302, 92/316, 92/368, 155, 155/217, 155/368, 166, 166/283/284, 166/302, 206, 206/217, 206/334, 261, 261/283, 271, 271/368, 275, 283, 283/284, 283/392, 284, 302, 316, 334, 339, 368, 368/392 및 392로부터 선택된 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하며, 상기 폴리펩타이드 서열의 아미노산 위치는 서열번호 1022를 기준으로 넘버링되는 것인 재조합 알파-갈락토시다제 A.
- 제1항에 있어서, 표 11.1, 12.1 및/또는 13.1에 제공된 바와 같은 적어도 하나의 위치에서 적어도 하나의 돌연변이를 포함하는 재조합 알파 갈락토시다제 A.
- 제1항 내지 제5항 중 어느 한 항에 있어서, 인간 알파-갈락토시다제 A로부터 유도되는 재조합 알파-갈락토시다제 A.
- 서열번호 374, 704 및/또는 1022의 폴리펩타이드 서열을 포함하는 재조합 알파-갈락토시다제 A.
- 제1항 내지 제7항 중 어느 한 항에 있어서, 서열번호 2, 374, 704 및/또는 1022의 알파-갈락토시다제 A보다 더 열안정한 재조합 알파-갈락토시다제 A.
- 제1항 내지 제8항 중 어느 한 항에 있어서, 서열번호 2, 374, 704 및/또는 1022의 알파-갈락토시다제 A보다 pH 7에서 더 안정한 재조합 알파-갈락토시다제 A.
- 제1항 내지 제9항 중 어느 한 항에 있어서, 서열번호 2, 374, 704 및/또는 1022의 알파-갈락토시다제 A보다 pH 4에서 더 안정한 재조합 알파-갈락토시다제 A.
- 제1항 내지 제10항 중 어느 한 항에 있어서, 서열번호 2, 374, 704 및/또는 1022의 알파-갈락토시다제 A보다 혈청에 대한 노출에 더 안정한 재조합 알파-갈락토시다제 A.
- 제1항 내지 제11항 중 어느 한 항에 있어서, 서열번호 2, 374, 704 및/또는 1022의 알파-갈락토시다제 A보다 라이소좀에서 더 안정한 재조합 알파-갈락토시다제 A.
- 제1항 내지 제12항 중 어느 한 항에 있어서, 서열번호 2, 374, 704 및/또는 1022의 알파-갈락토시다제 A보다 세포에 의해 더 용이하게 흡수되는 재조합 알파-갈락토시다제 A.
- 제1항 내지 제13항 중 어느 한 항에 있어서, 서열번호 2, 374, 704 및/또는 1022의 알파-갈락토시다제 A보다 세포로부터 더 많은 글로보트리아오실세라마이드를 고갈시키는 재조합 알파-갈락토시다제 A.
- 제1항 내지 제14항 중 어느 한 항에 있어서, 정제된 재조합 알파-갈락토시다제 A.
- 제1항 내지 제15항 중 어느 한 항에 있어서, 기준 서열에 비해 i) 향상된 촉매 활성; ii) pH 7에 대한 증가된 내성; iii) pH 4에 대한 증가된 내성; iv) 혈청에 대한 증가된 내성; v) 세포 내로의 증가된 흡수; vi) 세포로부터 글로보트리아오실세라마이드의 증가된 고갈; vii) 감소된 면역원성; 또는 i), ii), iii), iv), v), vi) 및/또는 vii) 중 임의의 성질의 조합으로부터 선택된 적어도 하나의 개선된 성질을 나타내는 재조합 알파-갈락토시다제 A.
- 제16항에 있어서, 상기 기준 서열은 서열번호 374, 704 및/또는 1022로부터 선택되는 것인 재조합 알파-갈락토시다제 A.
- 제1항 내지 제17항 중 어느 한 항의 적어도 하나의 재조합 알파-갈락토시다제 A를 포함하는 조성물.
- 제1항 내지 제18항 중 어느 한 항에 기재된 적어도 하나의 재조합 알파-갈락토시다제 A를 코딩하는 재조합 폴리뉴클레오타이드 서열.
- 제19항에 있어서, DNA, RNA 및 mRNA로부터 선택되는 재조합 폴리뉴클레오타이드 서열.
- 제20항에 있어서, 코돈-최적화되는 재조합 폴리뉴클레오타이드 서열.
- 제19항, 제20항 및/또는 제21항의 재조합 폴리뉴클레오타이드 서열을 포함하는 발현 벡터.
- 제22항에 있어서, 상기 재조합 폴리뉴클레오타이드 서열은 하나 이상의 조절 서열에 작동 가능하게 연결되는 것인 발현 벡터.
- 제23항에 있어서, 상기 조절 서열은 프로모터인 발현 벡터.
- 제24항에 있어서, 상기 프로모터는 이종 프로모터인 발현 벡터.
- 제22항 내지 제25항 중 어느 한 항의 발현 벡터를 포함하는 숙주 세포.
- 제26항에 있어서, 진핵세포 및 원핵세포로부터 선택되는 숙주 세포.
- 제26항 및/또는 제27항에 있어서, 포유류 세포인 숙주 세포.
- 알파-갈락토시다제 A 변이체의 제조 방법으로서, 제26항 내지 제28항 중 어느 한 항의 숙주 세포를 상기 재조합 폴리뉴클레오타이드에 의해 코딩된 상기 알파-갈락토시다제 A가 생성되는 조건 하에서 배양하는 단계를 포함하는 제조 방법.
- 제29항에 있어서, 상기 알파-갈락토시다제 A를 회수하는 단계를 추가로 포함하는 방법.
- 제30항에 있어서, 상기 알파-갈락토시다제 A를 정제하는 단계를 추가로 포함하는 방법.
- 제18항의 조성물을 포함하는, 파브리병의 치료를 위한 약학 조성물.
- 제32항에 있어서, 약학적으로 허용 가능한 담체 및/또는 부형제를 추가로 포함하는 약학 조성물.
- 제32항 및/또는 제33항에 있어서, 인간에게 비경구 주사되거나 주입되기에 적합한 약학 조성물.
- 제19항 내지 제21항 중 어느 한 항의 재조합 폴리뉴클레오타이드를 포함하는 약학 조성물.
- 대상체에서 파브리병의 증상을 치료 및/또는 예방하는 방법으로서, 파브리병을 가진 대상체를 제공하는 단계, 및 제32항 내지 제35항 중 어느 한 항의 약학 조성물을 상기 대상체에게 제공하는 단계를 포함하는 방법.
- 제36항에 있어서, 상기 파브리병의 증상이 호전되는 것인 방법.
- 제36항 및/또는 제37항에 있어서, 상기 대상체는 파브리병의 증상을 나타내는 대상체에 의해 요구된 식이보다 지방 함량이 덜 제한된 식이를 먹을 수 있는 것인 방법.
- 제36항 내지 제38항 중 어느 한 항에 있어서, 상기 대상체는 유아 또는 소아인 방법.
- 제36항 내지 제39항 중 어느 한 항에 있어서, 상기 대상체는 성인 또는 청년인 방법.
- 제18항 및 제32항 내지 제35항 중 어느 한 항에서 제공된 조성물의 용도.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US202062982949P | 2020-02-28 | 2020-02-28 | |
| US62/982,949 | 2020-02-28 | ||
| PCT/US2021/019811 WO2021173928A2 (en) | 2020-02-28 | 2021-02-26 | Human alpha-galactosidase variants |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| KR20220146601A true KR20220146601A (ko) | 2022-11-01 |
Family
ID=77463498
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1020227033613A KR20220146601A (ko) | 2020-02-28 | 2021-02-26 | 인간 알파-갈락토시다제 변이체 |
Country Status (17)
| Country | Link |
|---|---|
| US (1) | US20210269787A1 (ko) |
| EP (1) | EP4110926A2 (ko) |
| JP (1) | JP2023516301A (ko) |
| KR (1) | KR20220146601A (ko) |
| CN (1) | CN116096898A (ko) |
| AR (1) | AR121457A1 (ko) |
| AU (1) | AU2021228689A1 (ko) |
| BR (1) | BR112022016990A2 (ko) |
| CA (1) | CA3173294A1 (ko) |
| CL (1) | CL2022002330A1 (ko) |
| CO (1) | CO2022012809A2 (ko) |
| EC (1) | ECSP22075305A (ko) |
| IL (1) | IL295818A (ko) |
| MX (1) | MX2022010663A (ko) |
| PE (1) | PE20230487A1 (ko) |
| TW (1) | TW202146648A (ko) |
| WO (1) | WO2021173928A2 (ko) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3898960A4 (en) | 2018-12-20 | 2022-11-30 | Codexis, Inc. | VARIANTS OF HUMAN ALPHA GALACTOSIDASE |
| WO2024042485A1 (en) * | 2022-08-25 | 2024-02-29 | Takeda Pharmaceutical Company Limited | Composition for use in the treatment of fabry disease |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2361631A1 (en) * | 2002-04-25 | 2011-08-31 | Shire Human Genetic Therapies, Inc. | Treatment of alpha-galactosidase a deficiency |
| US9194011B2 (en) * | 2009-11-17 | 2015-11-24 | Protalix Ltd. | Stabilized alpha-galactosidase and uses thereof |
| EP4234699A1 (en) * | 2014-12-22 | 2023-08-30 | Codexis, Inc. | Human alpha-galactosidase variants |
| AU2020327019A1 (en) * | 2019-08-07 | 2022-03-03 | Amicus Therapeutics, Inc. | Methods of treating Fabry disease in patients having a mutation in the GLA gene |
-
2021
- 2021-02-26 AU AU2021228689A patent/AU2021228689A1/en active Pending
- 2021-02-26 CN CN202180017455.2A patent/CN116096898A/zh active Pending
- 2021-02-26 MX MX2022010663A patent/MX2022010663A/es unknown
- 2021-02-26 KR KR1020227033613A patent/KR20220146601A/ko unknown
- 2021-02-26 TW TW110107124A patent/TW202146648A/zh unknown
- 2021-02-26 BR BR112022016990A patent/BR112022016990A2/pt unknown
- 2021-02-26 EP EP21761023.7A patent/EP4110926A2/en active Pending
- 2021-02-26 CA CA3173294A patent/CA3173294A1/en active Pending
- 2021-02-26 WO PCT/US2021/019811 patent/WO2021173928A2/en active Application Filing
- 2021-02-26 US US17/186,462 patent/US20210269787A1/en active Pending
- 2021-02-26 JP JP2022551638A patent/JP2023516301A/ja active Pending
- 2021-02-26 IL IL295818A patent/IL295818A/en unknown
- 2021-02-26 AR ARP210100519A patent/AR121457A1/es unknown
- 2021-02-26 PE PE2022001808A patent/PE20230487A1/es unknown
-
2022
- 2022-08-25 CL CL2022002330A patent/CL2022002330A1/es unknown
- 2022-09-08 CO CONC2022/0012809A patent/CO2022012809A2/es unknown
- 2022-09-27 EC ECSENADI202275305A patent/ECSP22075305A/es unknown
Also Published As
| Publication number | Publication date |
|---|---|
| TW202146648A (zh) | 2021-12-16 |
| JP2023516301A (ja) | 2023-04-19 |
| BR112022016990A2 (pt) | 2022-10-25 |
| PE20230487A1 (es) | 2023-03-21 |
| EP4110926A2 (en) | 2023-01-04 |
| CA3173294A1 (en) | 2021-09-02 |
| CO2022012809A2 (es) | 2022-09-20 |
| IL295818A (en) | 2022-10-01 |
| WO2021173928A3 (en) | 2021-09-30 |
| AR121457A1 (es) | 2022-06-08 |
| CN116096898A (zh) | 2023-05-09 |
| AU2021228689A1 (en) | 2022-09-01 |
| US20210269787A1 (en) | 2021-09-02 |
| ECSP22075305A (es) | 2022-12-30 |
| CL2022002330A1 (es) | 2023-03-03 |
| MX2022010663A (es) | 2022-09-23 |
| WO2021173928A2 (en) | 2021-09-02 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US10973888B2 (en) | Human alpha-galactosidase variants | |
| US20220356458A1 (en) | Human alpha-galactosidase variants | |
| KR20220146601A (ko) | 인간 알파-갈락토시다제 변이체 | |
| US20240026326A1 (en) | Engineered amylase variants | |
| US11970722B2 (en) | Engineered acid alpha-glucosidase variants | |
| JP2024519906A (ja) | 操作されたメチオニンガンマリアーゼバリアント |