KR20220138241A - Functional cosmetic composition containing Bombus terrestris extract - Google Patents

Abstract

The present invention relates to a functional cosmetic composition containing a Bombus terrestris extract, and more particularly, to a functional cosmetic composition comprising an alcohol extract of Bombus terrestris as an active ingredient.

Description

Functional cosmetic composition containing Bombus terrestris extract

The present invention relates to a functional cosmetic composition containing an extract of sagebrush, and more particularly, to a multi-purpose functional cosmetic composition effective for collagen production, skin regeneration, wound recovery and skin moisturizing.

The most important role of the skin of animals including humans is to act as a defense barrier to protect the body from external damage and invasion of bacteria, and to prevent loss of heat and moisture from the inside. The skin with this function is composed of several layers, and the outermost one of them is called the epidermis, and it is known that the epidermis has regenerative ability in the basal layer of the skin keratinocytes (Keratinocytes). stem cells, KSC).

It is known that aging of the skin occurs due to cell mutation, active oxygen radicals, accumulation of toxic substances, and modification of skin interstitial proteins. The reason why scars remain when this occurs seems to be that the level of cell proliferation required for skin regeneration decreases significantly with age. That is, as aging progresses, the degree of proliferation of the cells is lowered, which is a decrease in skin regeneration ability, which may delay recovery of wounds or the like, or may cause wrinkles due to deepening of skin moisture and elasticity reduction.

In order to solve problems such as skin aging and wrinkle improvement, various studies on changes in collagen are being conducted. Low molecular weight compounds that promote collagen production are currently widely used retinoids to improve skin wrinkles, blemishes, and pigmentation, and among them, clinical results of skin wrinkle removal for retinol (vitamin A) have been reported variously. However, if retinol is used for dry skin, it may exfoliate the skin and cause severe skin irritation or dryness. In addition, in the case of recombinant protein raw materials, expensive costs are required, and in the case of natural extracts, there is a disadvantage in that the reproducibility is poor.

The hornbill bee has been mainly studied for medicinal use as glycozaminoglycan, a fraction of worker or queen bees. For example, a composition for enhancing immunity (Patent Registration No. 10-1403520) comprising, as an active ingredient, an alcohol extract of hornet bee, including western hornbee worker bee, has been disclosed, but its efficacy as a cosmetic is not known.

Therefore, in order to solve the side effects of various cosmetic compositions developed to prevent skin dryness and strengthen immune function, the present inventors have developed a cosmetic composition having skin moisturizing, collagen production and skin regeneration and wound healing components from the extract of sagebrush, which is a natural material. As a result of research and efforts to find the result, the present invention was completed.

One aspect of the present invention is to provide a functional cosmetic composition for multiple uses effective for collagen production, skin regeneration, wound recovery and skin moisturizing.

According to one aspect of the present invention, there is provided a functional cosmetic composition comprising an alcohol extract of sagebrush as an active ingredient.

Functional cosmetic composition containing locust serrata extract as an active ingredient can provide a functional cosmetic composition that is effective for skin regeneration, wound recovery and skin moisturizing by using natural ingredients that are safe for the skin. It can be used as an anti-pollution cosmetic that protects the skin from harmful environments such as fine dust.

1 shows the results of evaluation of cytotoxicity of extracts of honey bee and queen bee for human dermal fibroblasts.
FIG. 2 shows the results of confirming the effect of extracts of western hornet bee and queen bee on PIP (Type-1 Procollagen) production of human dermal fibroblasts.
Figure 3 shows the results of (a) cytotoxicity test, (b) hyaluronic acid and (c) ceramide kinase production experiment for confirming the condition of human dermal fibroblast damage caused by hydrogen peroxide (H ₂ O ₂ ).
Figure 4 is the result of confirming the effect of the extracts of western honey bee and queen bee on the production of (a) hyaluronic acid and (b) ceramide kinase in human dermal fibroblasts under oxidative damage conditions by hydrogen peroxide (500 μM H ₂ O ₂ ) treatment. is shown.
Figure 5 shows the effect of the extract of the queen bee of the Western Swamp bee on the cell migration (skin regeneration) of human dermal fibroblasts. The results of comparison of wound healing effects are shown.

Hereinafter, preferred embodiments of the present invention will be described with reference to the accompanying drawings. However, the embodiments of the present invention may be modified in various other forms, and the scope of the present invention is not limited to the embodiments described below.

According to the present invention, there is provided a functional cosmetic composition for multiple uses effective for collagen production, skin regeneration, wound recovery, and skin moisturizing, and more specifically, the functional cosmetic composition of the present invention includes an alcohol extract of sagebrush as an active ingredient.

The functional cosmetic composition of the present invention enhances skin elasticity by promoting collagen production, promotes skin regeneration by promoting cell migration, and/or hyaluronic acid (HA) and ceramide kinase ( Ceramide kinase, CERK) is stimulated to express complex functions that can enhance skin moisture.

The bombus terrestris, which can be used in the present invention, is an insect of the Hymenoptera family, originating in Europe, and introduced as a resource insect in Korea. In the present invention, the bristle bee may be at least one selected from the group consisting of a male bee, a worker bee, and a queen bee, and preferably a queen bee. The length of the queen bee is 20~22mm, that of the male bee is 14~16mm, and that of the worker bee is 11~17mm.

The alcohol for obtaining the alcohol extract of the locust may be at least one selected from the group consisting of alcohols having 1 to 3 carbon atoms, hexane and chloroform, for example, may be selected from methanol, ethanol and propanol, preferably For example, ethanol may be used. The concentration is preferably from 70 to 100% (v/v), for example from 70 to 95% (v/v).

The active ingredient may be included at a concentration of 15 to 500 μg/ml based on the volume of the functional cosmetic composition, for example, 30 to 250 μg/ml, or 60 to 250 μg/ml. When the active ingredient is less than 15 μg/ml, the collagen production, skin regeneration, wound recovery and skin moisturizing effects of the present invention may be insufficient, and when it exceeds 500 μg/ml, the increase in the effect compared to the increase in the content tends not to be large. have. However, the present invention is not limited thereto, and the effective amount may be appropriately selected depending on the individual. the severity of the skin condition, the subject's age, weight, health, sex, the subject's sensitivity to the extract, the time of administration, the route of administration and the rate of excretion, the duration of administration, factors including other compositions used in combination with or concurrently with the composition, and other physiology to factors well known in the medical field.

The functional cosmetic composition of the present invention may be formulated as a solution, suspension, emulsion, paste, gel, cream, powder or spray formulation together with a cosmetically or dermatologically acceptable carrier.

On the other hand, the cosmetic composition is at least one of preservatives, emulsifiers, stabilizers, surfactants, solubilizers, moisturizers, emollients, ultraviolet absorbers, preservatives, bactericides, antioxidants, pH adjusters, organic and inorganic pigments, fragrances, cooling agents and limiting agents. It may further include one or more components, but is not limited thereto. The blending amount of the additional ingredients such as the moisturizing agent can be easily selected by those skilled in the art within the range that does not impair the purpose and effect of the present invention, and the blending amount is 0.001 to 5% by weight, specifically 0.01 to 3% by weight, based on the total weight of the composition % by weight.

In the present invention, the skin includes all skin parts of the body including the face, hands, arms, legs, feet, chest, stomach, back, buttocks, and scalp.

Specifically, the functional cosmetic composition of the present invention may be prepared as a lotion, nourishing cream, lotion, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, soap or foundation.

When the formulation of the present invention is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as a carrier component. can

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In particular, in the case of a spray, additional chlorofluorohydrocarbon, propane /may contain propellants such as butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion (emulsion), a solvent, solubilizer or emulsifier is used as a carrier component, specifically water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, Propylene glycol, 1,3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol, fatty acid ester of sorbitan, and the like may be used.

When the formulation of the present invention is a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester, polyoxyethylene sorbitan ester, microcrystals Sex cellulose, aluminum metahydroxide, bentonite, agar, and the like can be used.

The alcohol extract of the locust larvae of the present invention may be obtained by filtering and concentrating the undiluted extract extracted at 5 to 35° C. using alcohol in a weight ratio of 2 to 30 times.

In more detail, prepare an extract stock solution extracted at room temperature, for example, 5 to 35° C., using alcohol in a weight ratio of 2 to 30 times, for example, 5 to 25 times, or 10 to 20 times. do. This extraction process may be performed for, for example, 12 hours to 3 days, preferably 24 hours to 48 hours.

Thereafter, the extract stock solution is filtered to obtain an extract solution excluding solids. At this time, the method of filtration is not particularly limited, for example, any solid-liquid separation method known in the art, such as filtration by filter paper, may be used.

Furthermore, the extract solution is recovered and concentrated to prepare an extract of the locust beetle. In this case, the concentration method is not particularly limited, and for example, concentration under reduced pressure may be performed.

The composition containing the extract of the present invention as an active ingredient is a functional cosmetic composition that is effective for skin regeneration, wound recovery and skin moisturizing using natural ingredients that are safe for skin and/or a pharmaceutical composition together with a pharmaceutically acceptable carrier. It is possible to use

Hereinafter, the present invention will be described in more detail through specific examples. The following examples are only examples to help the understanding of the present invention, and the scope of the present invention is not limited thereto.

Example

1. Preparation of extract

Example 1: Preparation of extract of queen bee queen bee

After washing 1 kg of queen bee with water twice, it was dehydrated using a sieve, dried at 60° C. for 12 hours, and then pulverized. As a result, the pulverized product was extracted at room temperature (20° C.) for 48 hours using 95% ethanol in a weight ratio of 20 times. This extract stock solution was filtered using filter paper (Wattman No 4), and then concentrated under reduced pressure to obtain an extract of the queen bee of the honey bee.

EXAMPLE 2: Preparation of extracts from hornbeam bee bee

Extraction was carried out in the same manner as in Example 1, except that the hornet wasp was used to obtain an extract of the hornbeam bee.

2. Cytotoxicity experiment

Human dermal fibroblasts (HDF) were cultured in DMEM medium supplemented with 10% fetal bovine serum (FBS), penicillin (100 U/ml), and streptomycin (100 μg/ml) at 37°C and 5% CO ₂ conditions. . The cells were cultured in a 75 cm ² flask, and the culture surface was washed with a phosphate buffered saline (PBS) solution every 3 days, and then the cells were detached and suspended in a DMEM medium supplemented with 10% FBS at a certain ratio. cm ² Transfer to a flask, 37 ℃, 5% CO ² Incubated in an incubator condition.

An MTT assay was performed to measure macrophage cytotoxicity of the queen bee extract of Example 1 and the queen bee extract of Example 2 and of Example 2. In more detail, human dermal fibroblasts were cultured in DMEM cell culture medium containing 10% FBS at 37° C. and 5% CO ₂ conditions, and then dispensed into 96 well plates to become 2×10 ⁴ cells, and for 12 hours. Cells were completely adhered while culturing, and the extracts were treated for 24 hours at different concentrations. Thereafter, 20 μl of MTT solution was added per well, followed by incubation for 2 hours, and after removing all of the culture supernatant, 200 μl of DMSO was added per well to form intracellular formazan ( formazan) was completely dissolved, and the absorbance was measured at 570 nm using a microplate reader. Cell viability was calculated as the absorbance % of the extract treated group compared to the untreated control group (CON), and the results are shown in FIG. 1 . In this case, the control group (CON) represents the untreated group without the addition of the dwarf beetle extract.

Referring to FIG. 1 , it was confirmed that all of the extracts of the present invention had no cytotoxicity up to a concentration of 500 μg/ml or less.

3. Collagen production experiment

In order to evaluate the effect of the honey bee extracts of the present invention on collagen biosynthesis, an experiment was performed to confirm the effect on PIP (Type-1 Procollagen) production of human dermal fibroblasts. In more detail, human dermal fibroblasts were cultured in DMEM cell culture medium containing 10% FBS at 37° C. and 5% CO ₂ conditions and then dispensed into 96 well plates to become 2×10 ⁴ cells, and for 12 hours. Cells were completely adhered while culturing, and cells in each well were washed with serum-free DMEM culture medium, and the extracts were treated in each well by concentration and cultured for 48 hours. Then, the culture medium was collected and the collagen content was measured using a Procollagen type I C-peptide assay kit (Takara Bio Inc.), and the concentration of collagen was calculated from the standard curve calculated from the standard solution included in the kit. At this time, the control group (CON) represents the untreated group without the addition of the extract, 'Adenosine' 500 ㎍ as a positive control group to compare the efficacy of the extract as a functional cosmetic ingredient that is effective for existing skin regeneration. /ml was used.

As a result, referring to FIG. 2 , it can be confirmed that the extract of the present invention increases the skin regeneration effect by PIP (collagen) production. It was confirmed that the skin regeneration effect was shown.

4. Hydrogen peroxide (H ₂ O ₂ ) Confirmation of human dermal fibroblast damage conditions

After inducing oxidative damage by treating human dermal fibroblasts with hydrogen peroxide (H ₂ O ₂ ) to induce skin cell damage for use as a skin damage model, (a) cytotoxicity, (b) hyaluronic acid production and (c) The amount of ceramide kinase (Ceramide Kinase) production was analyzed.

In more detail, cytotoxicity was analyzed in the same manner as in FIG. 1 . On the other hand, the amount of hyaluronic acid produced by culturing human dermal fibroblasts in a DMEM cell culture medium containing 10% FBS at 37 ° C., 5% CO ₂ conditions, 2 × 10 ⁴ cells were dispensed in a 96-well plate (well plate), Cells were completely attached while culturing for 12 hours, and hydrogen peroxide (H ₂ O ₂ ) was treated for 2 hours by concentration. Thereafter, the supernatant was collected and the amount of hyaluronic acid produced was measured with HA (Human Hyaluronic acid) ELISA Kit (CUSABIO). In the case of the amount of ceramide kinase produced, hydrogen peroxide (H ₂ O ₂ ) was treated to human dermal fibroblasts by concentration in the same manner as the hyaluronic acid analysis method, and then the supernatant and the cells were separated to compare the extracellular and intracellular contents. The obtained cells were treated with RIPA Lysis Buffer (BIOMAX), and the supernatant was collected by centrifugation at 1,800 rpm for 5 minutes to obtain a cell lysate sample. Afterwards, the supernatant and cell lysate samples obtained were compared and analyzed for the amount of ceramide kinase produced in the supernatant and cells using CERK (Human Ceramide Kinase) ELISA Kit (CUSABIO).

As a result, as can be seen in FIG. 3 , as a result of treating human dermal fibroblasts with hydrogen peroxide (H ₂ O ₂ ), the cell viability was rapidly reduced due to oxidative damage from a concentration of 500 μM, and at the same time, human dermal fibroblasts produced It was found that the production of hyaluronic acid and ceramide kinase, which are components of the skin, decreased together. As a result, it was confirmed that the 500 μM concentration of hydrogen peroxide (H ₂ O ₂ ) treatment was the optimal condition to damage human dermal fibroblasts. In this case, the control group (CON) represents an untreated group that is not treated with hydrogen peroxide (H ₂ O ₂ ).

5. Identification of the effect of the extract of the present invention under oxidative damage conditions

In order to check whether the extracts of the honey bee and queen bee are effective in oxidative skin damage caused by hydrogen peroxide, after culturing human dermal fibroblasts in the same manner as in 4. during pretreatment. Thereafter, based on the confirmation in 4. above, human dermal fibroblasts were treated with 500 μM hydrogen peroxide for 2 hours to compare and analyze hyaluronic acid production and intracellular ceramide kinase production, and the results are shown in FIG. 4 . In this case, the control group (CON) represents an untreated group that was not treated with the extract and hydrogen peroxide (H ₂ O ₂ ).

As a result, as can be seen in FIG. 4, it was possible to confirm an increase in the production of hyaluronic acid (FIG. 4(a)) and ceramide kinase (FIG. 4(b)) by the extract of the present invention, especially when the queen bee extract was used. It has been found that a significant increase in their production can be obtained. On the other hand, when only hydrogen peroxide was treated, the production of hyaluronic acid and ceramide kinase was rapidly decreased compared to the control group (CON) due to oxidative damage. It was found that the production of hyaluronic acid and ceramide phosphorylation enzymes increased, thereby reducing the oxidative damage caused by hydrogen peroxide.

In particular, in the case of the queen bee extract, it was confirmed that the amount of hyaluronic acid and ceramide, which is effective for skin moisturizing, as a component of the skin, was further increased compared to that of the male bee, and the effect of preventing oxidative damage to human dermal fibroblasts caused by hydrogen peroxide was confirmed to be greater.

6. Confirmation of skin regeneration effect of the extract of the present invention

In order to confirm the effect of the queen bee extract according to the present invention on the wound healing ability through cell migration of human dermal fibroblasts, that is, skin regeneration, in more detail, DMEM containing 10% FBS of human dermal fibroblasts. After culturing in a cell culture medium at 37° C. and 5% CO ₂ conditions, the cells were aliquoted into 6 well plates to become 5×10 ⁵ cells, and the cells were completely adhered while culturing for 12 hours. After that, the center of the well plate was scraped using a scratcher (SPL Inc.) to make a wound, and then, after 8 hours of treatment with the extract of the queen bee extract at each concentration, the length between the wounds was measured through microscopic observation (Wound closure length). The degree of wound healing was analyzed. At this time, the control group (CON) represents the untreated group without the addition of the extract, 'Adenosine' 500 μg/ ml was used.

As a result, as can be seen in FIG. 5 , when the skin cells were wounded, treated with the western hummingbird extract and cultured for 8 hours, compared to the control (CON), the western hummingbird extract was treated, wound recovery through skin regeneration. This rapid thing could be confirmed with a micrograph (FIG. 5(a)), and the wound healing effect could be confirmed by measuring the length of the wound (FIG. 5(b)).

Although the embodiments of the present invention have been described in detail above, the scope of the present invention is not limited thereto, and various modifications and variations are possible within the scope without departing from the technical spirit of the present invention described in the claims. It will be apparent to those of ordinary skill in the art.

Claims (10)

A functional cosmetic composition comprising, as an active ingredient, an alcohol extract of sagebrush.
The functional cosmetic composition according to claim 1, wherein the functional cosmetic composition enhances skin elasticity by promoting collagen production.
The functional cosmetic composition of claim 1, wherein the functional cosmetic composition promotes skin regeneration and wound recovery by promoting cell migration.
The functional cosmetic composition of claim 1, wherein the functional cosmetic composition enhances skin moisture by promoting secretion of hyaluronic acid (HA) and ceramide kinase (CERK).
The functional cosmetic composition according to claim 1, wherein the locust bee is at least one selected from the group consisting of a male bee, a worker bee, and a queen bee.
The functional cosmetic composition according to claim 1, wherein the alcohol is at least one selected from the group consisting of alcohols having 1 to 3 carbon atoms, hexane and chloroform.
The functional cosmetic composition according to claim 1, wherein the active ingredient is included in a concentration of 15 to 500 μg/ml based on the volume of the functional cosmetic composition.
According to claim 1, wherein the functional cosmetic composition is a solution, suspension, emulsion, paste, gel, cream, powder or spray formulation, the functional cosmetic composition.
The functional cosmetic composition of claim 1, wherein the functional cosmetic composition is a lotion, lotion, nourishing cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, soap or foundation.
The functional cosmetic composition according to claim 1, wherein the alcohol extract of locust beetle is obtained by filtering and concentrating the extract extracted at 5 to 35°C using alcohol in a weight ratio of 2 to 30 times.

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