WO2022055034A1 - Functional cosmetic composition using nordenau water - Google Patents

Abstract

The present invention relates to a functional cosmetic composition using Nordenau water. Specifically, the present invention provides a functional cosmetic composition which has an anti-wrinkle or whitening effect, and comprises: Nordenau water; a human stem cell-derived culture medium extract; a Centella asiatica extract; and purified water. The cosmetic composition contains, as active ingredients, natural substances such as: Nordenau water, which is rich in minerals and has excellent antioxidant effects; a human stem cell-derived culture extract, which has excellent effects on cell regeneration and anti-aging; and a Centella asiatica extract, and thus is safe and has few side effects even after long-term use, and can exhibit an even more enhanced anti-wrinkle or whitening effect.

Description

Functional cosmetic composition using Nordenau water

The present invention relates to a functional cosmetic composition using Nordenau water, and more particularly, to a functional cosmetic composition having a wrinkle improvement or whitening effect, comprising Nordenau water, human stem cell culture extract, Centella asiatica extract and purified water.

As the largest organ in the body, the skin is always in direct contact with the external environment and acts as a protective film to protect the living body from various stimuli or dry environments. Various physical factors, external environmental factors, infections, etc. bring about skin changes such as skin aging, skin contamination, dryness, troubles, and pigmentation.

As the quality of life of modern people has improved and people's interest in their appearance has increased, the demand for cosmetics has surged. there is.

In addition, in recent years, as part of the development of materials with less possibility of side effects due to various problems such as toxicity, low activity, limitation of use, and side effects due to overdose of existing chemicals, functions such as wrinkle relief and whitening obtained from natural products have been improved. Research and development of functional cosmetics with Nature-friendly natural materials are safe and have few side effects on the skin, and as consumers' response to cosmetics using natural materials increases, the development value as a raw material for functional cosmetic compositions is further increasing.

It is an object of the present invention to provide a functional cosmetic composition comprising a natural product having an excellent effect on wrinkle improvement or skin whitening as an active ingredient.

In order to solve the above object, the present invention provides a functional cosmetic composition comprising Nordenau water, human stem cell culture extract, Centella asiatica extract and purified water.

The cosmetic composition according to the present invention contains, as active ingredients, natural products such as Nordenau water, which is rich in minerals and has excellent antioxidant effects, human stem cell culture extract and Centella asiatica extract, which are excellent in cell regeneration and anti-aging effects, as active ingredients. It is safe with few side effects, and it can exhibit improved wrinkle improvement or whitening effect.

1 is a graph showing the cell proliferation effect according to an experimental example of the present invention.

2 is a graph showing the collagen synthesis effect according to an experimental example of the present invention.

3 and 4 are graphs showing the effect of inhibiting melanin synthesis and tyrosinase enzyme activity according to an experimental example of the present invention.

5 is a graph showing the whitening improvement effect according to an experimental example of the present invention.

Hereinafter, the present invention will be described in detail.

The present invention provides a functional cosmetic composition comprising Nordenau water, human stem cell culture extract, Centella asiatica extract and purified water.

As used herein, "Nordenau water" is mineral water flowing from the basement of the Nordenau Schist (Tomes Cave), about 200 km away from Frankfurt, Germany. It is a story that various diseases were cured by drinking this water. It is known as a miracle water and one of the world's four most famous waters. Nordenau water is rich in minerals (calcium, potassium, magnesium) and has excellent ability to remove active oxygen.

As used herein, the term "human-derived stem cells" refers to stem cells obtained from adults other than embryonic stem cells, and for example, adipose tissue, bone marrow, umbilical cord blood, and peripheral blood, including blood and tissue basal. Includes all stem cells originating from

Preferably, the human-derived stem cell culture solution extract is a human adipocyte conditioned media extract, specifically, the culture medium obtained by culturing human-derived adipose stem cells and the cultured adipose stem cells are disrupted. It may include an extract obtained by

The culture solution extract promotes the proliferation of fibroblasts that synthesize collagen and induces vascular endothelial growth factor (VEGF), which induces the secretion of new blood vessels and other regeneration promoting factors, induces cell activity and makes skin color. Hepatocyte growth factor (HGF) that promotes division, insulin-like growth factor (IGF) that promotes cell regeneration and growth, and endothelial growth factor that enhances skin tissue regeneration and function ( Epidermal growth factor, EGF, may include 150 kinds of growth factors such as fibroblast growth factor (FGF) that induces collagen synthesis, collagen, cytokine ), may contain a large amount of extracellular matrix proteins such as fibronectin.

The culture medium extract can have a long-term skin improvement effect by inducing cell division when absorbed into the skin, and has an effect on aging through cell regeneration or restoration.

In addition, as a type of adult stem cells, adipose stem cells are easier to collect than bone marrow stem cells or umbilical cord blood stem cells, and have the advantage of being able to mass-cultivate and contain a large amount of stem cells.

As used herein, " Centella asiatica " is a dicotyledonous plant commonly found in the mountains or fields of the southern island of the Korean Peninsula. Because of the medecassic component in the leaves and stems of Centella asiatica, it is effective in wound healing, especially in skin regeneration, anti-wrinkle, blood circulation, anti-inflammatory, soothing, anti-aging due to antioxidant action, elasticity improvement, and prevention of pigmentation. etc. are also effective.

In the present invention, the Centella asiatica extract may be extracted with water, C1 to C4 alcohol or a mixed solvent thereof, and may preferably be a hot water extract, but is not limited thereto.

The Centella asiatica extract may be obtained by extracting the roots, stems, leaves, flowers, fruits, or a mixture of these parts of Centella asiatica, and the Centella asiatica may be collected or cultivated in nature, or purchased commercially.

As used herein, the term "extract" refers to a substance obtained by extracting a component of a natural product regardless of an extraction method, an extraction solvent, an extracted component, or the form of an extract. It may include any material obtainable by processing or processing by the method.

The extract may be extracted according to a method commonly used in the art, for example, hot water extraction method, ultrasonic extraction method, filtration method, reflux extraction method, etc., these are performed alone or in combination of two or more methods. can be

As used herein, "purified water" refers to water made by distilling water or filtration through chemical and physical methods, and is clean water free of solid particles, microorganisms, organic matter, fusion ions, etc. contained in water. do. As a major component of cosmetics, it is used to prevent discoloration, deterioration, and contamination of cosmetics.

In the present invention, the composition may include 20 to 40% by weight of Nordenau water, 15 to 30% by weight of human stem cell culture extract, 0.005 to 0.1% by weight of Centella asiatica extract, and the remaining amount of purified water.

The composition may further include one or more components selected from the group consisting of allantoin, hyaluronic acid, niacinamide, acetylhexapeptide-8, and extract of horseradish.

In the present invention, the composition may be provided to the skin in the form of nanoparticles with an average diameter of 0.1 to 100 nm, but is not limited thereto.

By providing the composition as nano-sized particles, it is possible to more easily pass through the skin cell gap, thereby increasing the skin absorption rate of the composition.

In the present specification, the term "cosmetics" may include functional cosmetics having an effect of preventing or improving skin aging, etc., and the functional cosmetics have specialized functionalities that emphasize physiologically active efficacy and effects, unlike general cosmetics. , refers to cosmetics emphasizing specific efficacy and effects, such as skin wrinkle improvement and whitening, as defined in the Cosmetics Act.

In the present invention, the composition may have a wrinkle improvement effect.

As used herein, "wrinkle improvement" means suppressing or inhibiting the generation of wrinkles on the skin, or alleviating the already generated wrinkles.

The anti-wrinkle effect may include a conventional skin protection effect such as preventing or improving skin wrinkles, inhibiting or improving skin aging. In addition, since collagen is involved in the mechanical strength of the skin, the bonding strength and elasticity of tissues, etc., effects such as improvement of skin elasticity, skin regeneration, wound healing, and repair and regeneration of damaged skin tissue can be obtained by the composition.

In the present invention, the composition may have a whitening effect.

As used herein, "whitening" refers to an action of improving skin troubles by inhibiting, inhibiting, or alleviating excessive deposition of melanin pigment and the like, thereby preventing skin blackening caused by the pigmentation.

The cosmetic composition according to the present invention may further include one or more of the ingredients listed in the list of cosmetic raw materials registered with the Ministry of Food and Drug Safety and ICID (International Cosmetic Ingredients). For example, the cosmetic composition may include an organic solvent, a solubilizer, a thickener, a gelling agent, an emollient, an antioxidant, a suspending agent, a stabilizer, a fragrance, a surfactant, an emulsifier, a filler, a sequestering agent, a preservative, a vitamin, a blocking agent, It may further comprise one or more adjuvants commonly used in the cosmetic field, such as wetting agents, dyes and pigments, hydrophilic or lipophilic actives, or any other ingredients commonly used in cosmetics.

The antioxidant prevents oxidation of oily components of cosmetics to maintain the quality, preservation, and safety of cosmetics, tocopherol, sodium sulfite, sodium hydrogen sulfite, sodium metabisulfite, potassium sulfite, potassium hydride Rosensulfite, potassium metabisulfite, butylated hydroxytoluene, butylhydroxyanisole, ascorbic acid, ascorbyl palmitate, etc. may include, but are not limited to, various types known in the art. Antioxidants may be used.

The vitamin may include a water-soluble or oil-soluble vitamin, and the water-soluble vitamin is a water-soluble vitamin that can be formulated in cosmetics, preferably vitamin B1, vitamin B2, vitamin B6, pyridoxine, pyridoxine hydrochloride, vitamin B12, pantothenic acid, nicotinic acid, and nicotinic acid amide, folic acid, vitamin C, vitamin H, and the like, and more preferably vitamin C, which helps collagen synthesis. In addition, salts thereof (thiamine hydrochloride, sodium ascorbate salt, etc.) or derivatives (ascorbic acid-2-phosphate sodium salt, ascorbic acid-2-phosphate magnesium salt, etc.) may be included, and the water-soluble vitamin is a microorganism transformation method , can be obtained by a conventional method such as a purification method from a culture of a microorganism, an enzymatic method or a chemical synthesis method.

The cosmetic composition according to the present invention may be prepared in any formulation conventionally prepared in the technical field to which the present invention pertains. For example, to be formulated into lotions, emulsions, skins, lotions, essences, ampoules, creams, pastes, gels, solutions, suspensions, oils, waxes, packs, powders, foundations, sprays, surfactant-containing cleansing, soaps, etc. However, the present invention is not limited thereto.

More specifically, flexible lotion, nourishing lotion, astringent lotion, nourishing cream, massage cream, milk lotion, powder, essence, eye cream, sun lotion, sun cream, makeup primer, makeup base, BB cream, powder foundation, emulsion foundation , cleansing cream, cleansing foam, cleansing water, soap, pack, stick type product, balm type product, spray or powder formulation.

When the cosmetic composition according to the present invention is a cream or gel formulation, animal oil, vegetable oil, wax, paraffin, starch, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be further included as a carrier component. can

When the cosmetic composition is a solution or emulsion formulation, a solvent, solvating agent or emulsifying agent, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, propylene glycol, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid It may further include esters and the like.

When the cosmetic composition is a suspension formulation, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystalline Cellulose, aluminum metahydroxide, bentonite, agar or tracanth may be further included.

When the cosmetic composition is in a powder or spray formulation, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be further included as a carrier component, and in particular, in the case of a spray formulation, additional chlorofluorohydrocarbon , may further include a propellant such as propane/butane or dimethyl ether.

When the cosmetic composition is a surfactant-containing cleansing formulation, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarcosinate, fatty acid amide as carrier components Ether sulfate, alkylamidobetaine, fatty alcohol, fatty acid glycerides, fatty acid diethanolamide, vegetable oil, linoline derivatives or ethoxylated glycerol fatty acid esters may be further included.

The cosmetic composition according to the present invention may be applied alone or in overlapping use, or may be used by overlapping application with other cosmetic compositions other than the present invention. In addition, the cosmetic composition according to the present invention can be used according to a conventional method of use, and the number of times of use can be varied according to the skin condition or taste of the user.

Hereinafter, examples will be described in detail to help the understanding of the present invention. However, the following examples are merely illustrative of the content of the present invention, and the scope of the present invention is not limited to the following examples. The embodiments of the present invention are provided to more completely explain the present invention to those of ordinary skill in the art.

<Example 1> Preparation of cosmetic composition comprising Nordenau water, human stem cell culture extract and Centella asiatica extract

Nordenau water, a German medical mineral water, was prepared, and human adipose-derived mesenchymal stem cells-conditioned medium (ADSC-CM) extract and Centella asiatica extract were prepared.

Adipose stem cells are separated from adipose tissue obtained through liposuction, suspended in a substrate medium, inoculated in a culture vessel, and cultured in an incubator at 37° C., CO ₂ for 24 hours, then the substrate medium is removed and a differentiation medium is added 3 After culturing for one day, adipocyte media was added and the cells were differentiated into adipocytes. The culture solution obtained in this process was separated and purified and used as an extract of the adipose tissue-derived stem cell culture solution of the present invention.

Centella asiatica was collected, washed, dried, and then pulverized. 100 g of pulverized Centella asiatica was added to 2 L of distilled water and heated at about 95° C. for 3 hours, followed by hot water extraction. After filtration, the resultant was concentrated under reduced pressure at 40° C. or lower, and then freeze-dried.

A cosmetic composition (Example 1) containing Nordenau water, human adipose tissue-derived stem cell culture extract, and Centella asiatica extract prepared according to the above was prepared with the components and contents of Table 1.

ingredient	Content (w/w)%	function
Nordenausu	30.00000000	antioxidant
Human adipocyte culture extract	20.00000000	Skin regeneration, collagen synthesis, etc.
glycerin	5.00490000	moisturizer
methyl propanediol	3.20000000	solvent, solvent, humectant
1,2-Hexanediol	2.07446150	Antioxidant, preservative, moisturizer, etc.
niacinamide	2.00000000	whitening agent
Centella asiatica extract	0.02362000	skin regeneration. antioxidant, etc.
allantoin	0.10000000	skin conditioning
Ascorbyl propyl hyalurate	0.00000060	Moisturizer, antioxidant, etc.
Acetylhexapeptide-8	0.00001000	anti aging
Purified water	remaining amount	solvent

<Experimental Example 1> Confirmation of cell proliferation effect

Human dermal fibroblast (HDF) cells, which are human dermal fibroblasts purchased from ATCC, were aliquoted at 5×10 ³ cells/well in a 96 well plate, and 0.5% FBS (fetal bovine serum) was added at 37° C., 5% CO ₂ . It was cultured for 24 hours in DMEM (Invitrogen-Gibco-BRL, Grand Island, NY) medium. In Example 1 and Comparative Example diluted with a culture medium to a final concentration of 1% (v/v), 50 μg/ml of an epidermal growth factor (EGF) sample was added and cultured for 24 hours. 10 μl of CCK-8 (Cell counting kit-8, Dojindo, Gaithersburg, MD, USA) solution was added to each well and incubated for 2 hours. The absorbance was measured at 450 nm using a microplate reader.

As a result, as shown in FIG. 1, the composition of Example 1 exhibited a more improved cell proliferation effect than the growth factor EGF.

<Experimental Example 2> Comparison of collagen synthesis amount

In order to confirm the collagen synthesis promoting effect of the cosmetic composition containing the stem cell culture medium of another company and the composition of Example 1 according to the present invention, a Procollagen Type I C-Peptide Enzyme Immuno Assay experiment was performed.

HDF cells were aliquoted in a 96-well plate at 5×10 ³ cells/well, cultured in DMEM medium at 37° C. for 24 hours, and then treated with a cosmetic composition of another company and Example 1 according to the present invention at 50 μg/ml. Further incubation in serum-free medium for the next 48 hours. After incubation, the amount of collagen synthesis was measured by collecting the supernatant of each well and measuring the amount of procollagen type I C-peptide (PICP) using a kit (Takara, JAPAN).

As a result, as shown in FIG. 2 , it was confirmed that Example 1 had a higher collagen synthesis amount than that of other stem cell cultures.

<Experimental Example 3> Comparison of melanin content and tyrosinase activity

Melanin content was measured by dispensing B16F10 melanoma cells distributed from ATCC at 2×10 ⁴ cells/well in a 24-well plate, and cultured at 37° C., 5% CO ₂ for 18 hours, and then the culture medium was removed. In order to induce the biosynthesis of melanin, the culture medium containing 0.2 ppm α-MSH (α-melanocyte stimulating hormone, Sigma-Aldrich) was exchanged, and each sample was added by concentration and cultured for 24 hours. After removing the medium and dispensing a certain amount of PBS so that the bottom surface is evenly spread, the cells are recovered by treatment with trypsin-EDTA, and the melanin content in the cells is measured by ELISA for absorbance at 405 nm. was measured.

As a result, as shown in FIGS. 3 and 4, when Example 1 was treated, melanin production and tyrosinase enzyme activity were significantly inhibited as the concentration increased compared to the case of α-MSH alone treatment.

In addition, when the concentration of Example 1 is 50 μg / ml, it is shown that the inhibitory effect of melanin and tyrosinase enzyme activity is superior to that of arbutin, a positive control known as a conventional tyrosinase inhibitor.

Hereinafter, a formulation example of a cosmetic composition comprising Nordenau water, a human adipose tissue-derived stem cell culture extract, and Centella asiatica extract according to the present invention will be described, but the present invention is not intended to limit it, but to describe it in detail.

<Preparation Example 1> Preparation of functional ampoule containing Nordenau water, human adipose tissue-derived stem cell culture medium extract, and Centella asiatica extract

Table 2 below shows the ingredients and contents of functional ampoules containing Nordenau water, human adipose tissue-derived stem cell culture extract, and Centella asiatica extract as active ingredients according to an embodiment of the present invention. A functional ampoule was prepared using the nano-size particle manufacturing method.

ingredient	Content (w/w)%
Nordenausu	30.00
Human fat cell culture solution extract	20.00
glycerin	5.00
methyl propanediol	3.20
1,2-Hexanediol	2.07
Butylene Glycol	0.70
Ceramide NP	0.50
betaine	0.30
Sodium Hyaluronate	0.05
Centella asiatica extract	0.08
Machiba extract	0.06
coconut palm oil	0.08
sunflower seed oil	0.08
Glyceryl Acrylate/Acrylic Acid Copolymer	0.01
Caprylic/Capric Triglycerides	0.05
allantoin	0.10
Octyldodeces-16	0.15
Sodium Coco-Sebum - Dimonium Chloride Phosphate	0.10
Disodium EDIT	0.02
adenosine	0.04
tromethamine	0.04
Purified water	remaining amount

<Experimental Example 4> Sensory evaluation

In order to evaluate the feeling of use of the functional ampoule in Preparation Example 1, sensory tests for absorption, moisturizing power, skin stability (lifting feeling, etc.), flavor, and overall satisfaction were performed. For 20 adult women (44 to 61 years old), the functional ampoule of Preparation Example 1 and its existing ampoule were evaluated for absorption, moisturizing power, skin stability, flavor and overall satisfaction on a scale of 1 to 10, and the The results are shown in Table 3 below.

In Table 3 below, each index has an excellent effect as its numerical value is higher.

	Existing own ampoules	Preparation Example 1
absorptivity	6.2	8.4
moisturizing power	6.0	9.1
Skin stability (lifting)	6.1	8.8
flavor	5.5	8.3
overall satisfaction	6	8.7

Referring to Table 3, it can be seen that the functional ampoule of Preparation Example 1 according to the present invention is evaluated more positively than the existing ampoules.

In addition, as a result of numerically confirming the whitening improvement effect at the 4th week and the 8th week of using the functional ampoule of Preparation Example 1 for the adult female, an improvement effect that can be confirmed visually as shown in FIG. 5 was shown.

As described above in detail a specific part of the content of the present invention, for those of ordinary skill in the art, it is clear that this specific description is only a preferred embodiment, and the scope of the present invention is not limited thereby. Do. That is, the substantial scope of the present invention is defined by the appended claims and their equivalents.

Claims (11)

1. Functional cosmetic composition comprising Nordenau water, human stem cell culture extract, Centella asiatica extract and purified water.
2. The method of claim 1,

   The composition is

   Nordenau water 20 to 40% by weight, human stem cell culture extract 15 to 30% by weight, Centella asiatica extract 0.005 to 0.1% by weight Functional cosmetic composition comprising the remaining amount of purified water.
3. The method of claim 1,

   The Nordenau number is

   A functional cosmetic composition, characterized in that it is mineral water from Tomes Cave in Nordenau, Germany.
4. The method of claim 1,

   The human-derived stem cell culture solution extract,

   A functional cosmetic composition comprising a culture solution obtained by culturing human adipose stem cells and an extract obtained by crushing the cultured adipose stem cells.
5. The method of claim 1,

   The Centella asiatica extract is

   A functional cosmetic composition, characterized in that it is a hot water extract of Centella asiatica.
6. The method of claim 1,

   The composition is

   A functional cosmetic composition, characterized in that it is provided to the skin in the form of nanoparticles with an average diameter of 0.1 to 100 nm.
7. The method of claim 1,

   The composition is

   A functional cosmetic composition, characterized in that it has a wrinkle-improving effect.
8. The method of claim 1,

   The composition is

   A functional cosmetic composition, characterized in that it has a whitening effect.
9. The method of claim 1,

   The composition is

   Functional cosmetic composition, characterized in that it further comprises one or more ingredients selected from the group consisting of allantoin, hyaluronic acid, niacinamide, acetyl hexapeptide-8, and Machihyun extract.
10. The method of claim 1,

    The composition is

    Tocopherol, sodium sulfite, sodium hydrogensulfite, sodium metabisulfite, potassium sulfite, potassium hydrogensulfite, potassium metabisulfite, butylated hydroxytoluene, butylhydroxyanisole, ascorbic acid and Functional cosmetic composition, characterized in that it further comprises one or more antioxidants selected from the group consisting of ascorbyl palmitate.
11. The method of claim 1,

    The composition is

    A functional cosmetic composition, characterized in that it is prepared in skin, lotion, essence, ampoule, cream, gel or soap.

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