KR20220138230A - Biomarkers for predicting prognosis of cancer - Google Patents

Abstract

The present invention relates to a composition for predicting the prognosis of cancer patients, including: NFX1 genes or protein thereof; MFN2 genes or protein thereof; and an agent for measuring the expression level of at least one of the same. The present invention can accurately predict treatment responsiveness, and accordingly, an optimal treatment plan for cancer patients can be established.

Description

Biomarkers for predicting prognosis of cancer

The present invention relates to a biomarker for predicting the prognosis of cancer patients.

In general, cancer chemotherapy using anticancer drugs has been one of the major medical advances in the past few decades. Traditional cancer chemotherapy relies on the premise that rapidly growing cancer cells are more likely to be killed by cytotoxic agents. There is this.

Accordingly, in order to overcome the above problems, mitochondria have emerged as an effective target in anticancer therapy (Don, 2004). Mitochondria are multifunctional organelles capable of morphological changes, and their activity is directly related to cell physiology (Newmeyer, 2003). For example, fragmented mitochondrial morphology is associated with apoptotic cytochrome c release. On the other hand, the tubular morphology has the dynamics of enhancing resistance to apoptosis stimuli. In addition, mitochondria not only function as a major component of cell survival through ATP production, but also determine cell fate by mitochondrial membrane-dependent apoptosis signaling (Karbowski, 2003).

Oxidative phosphorylation (OXPHOS) is a process of ATP synthesis through electron transfer and chemical osmosis in mitochondria. Almost all oxygen-breathing organisms perform oxidative phosphorylation. Oxidative phosphorylation is widely spread in nature because it is a very efficient method of synthesizing ATP from the energy released when the respiratory substrate is degraded compared to the anaerobic fermentation process. An anticancer agent having an anticancer effect by inhibiting such oxidative phosphorylation is called an OXPHOS inhibitor or an OXPHOS inhibitor.

On the other hand, cancer cells may show resistance to anticancer agents or targeted therapeutics due to their characteristics. Therefore, a marker for improving the actual therapeutic effect of cancer and particularly for confirming the reactivity of an anticancer drug, in particular a marker for preselecting patients who are reactive to an OXPHOS inhibitor (oxidative phosphorylation inhibitor) has not been discovered at present.

One object of the present invention is to provide a biomarker composition for predicting cancer prognosis.

Another object of the present invention is to provide a composition for predicting cancer prognosis.

Another object of the present invention is to provide a kit for predicting cancer prognosis.

Another object of the present invention is to provide a method for providing information for predicting a cancer prognosis.

However, the technical task to be achieved by the present invention is not limited to the tasks mentioned above, and other tasks not mentioned will be clearly understood by those of ordinary skill in the art from the following description.

BRIEF DESCRIPTION OF THE DRAWINGS Various embodiments described herein are described below with reference to the drawings. In the following description, various specific details are set forth, such as specific forms, compositions and processes, and the like, for a thorough understanding of the present invention. However, certain embodiments may be practiced without one or more of these specific details, or in conjunction with other known methods and forms. In other instances, well-known processes and manufacturing techniques have not been described in specific detail in order not to unnecessarily obscure the present invention. Reference throughout this specification to “one embodiment” or “an embodiment” means that a particular feature, form, composition, or characteristic described in connection with the embodiment is included in one or more embodiments of the invention. Thus, references to "in one embodiment" or "an embodiment" in various places throughout this specification do not necessarily refer to the same embodiment of the invention. Additionally, the particular features, forms, compositions, or characteristics may be combined in any suitable manner in one or more embodiments.

Unless otherwise defined in the present invention, all scientific and technical terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs.

In the present invention, "tumor" or "cancer" is a disease in which the cell cycle is not regulated and continues to divide, and is divided into Carcinoma and Sarcoma depending on the site of occurrence. Carcinoma refers to a malignant tumor that arises from epithelial cells such as mucous membranes and skin, and sarcoma refers to a malignant tumor that arises from non-epithelial cells such as muscle, connective tissue, bone, cartilage, and blood vessels.

In the present invention, the cancer is breast cancer, uterine cancer, esophageal cancer, stomach cancer, brain cancer, rectal cancer, colorectal cancer, lung cancer, skin cancer, ovarian cancer, cervical cancer, kidney cancer, blood cancer, pancreatic cancer, prostate cancer, testicular cancer, laryngeal cancer, oral cancer, head and neck cancer , thyroid cancer, liver cancer, bladder cancer, osteosarcoma, lymphoma, leukemia, thymus cancer, squamous cell carcinoma, adenocarcinoma, and a combination thereof may be a cancer selected from the group consisting of, and the progression of cancer such as tumor differentiation and / or proliferation in the present invention The type of cancer dependent on the described cancer cell is not limited thereto.

The term "prediction of prognosis" of the present invention refers to an act of predicting in advance the course of a disease and the outcome of death or survival. The prognosis or prognostic diagnosis may be interpreted to mean any act of predicting the course of a disease before/after treatment by comprehensively considering the patient's condition, as the course of the disease may vary depending on the physiological or environmental condition of the patient. can For the purpose of the present invention, the cancer prognosis is the survival rate or survival period of a cancer patient, or an anticancer drug treatment performed on a cancer patient, preferably an OXPHOS inhibitor and/or irinotecan treatment, and an act of predicting the therapeutic response in advance, or Based on this, it can be interpreted as an act of appropriately selecting whether to use an OXPHOS inhibitor and/or irinotecan.

According to one embodiment of the present invention, NFX1 (Transcriptional repressor NF-X1, X-box binding 1) gene or a protein encoded by it; and MFN2 (Mitofusin-2) gene or a protein encoded by it; It relates to a biomarker composition for predicting cancer prognosis, comprising at least one of.

In the present invention, "biomarker" is a biological indicator that can detect changes in the body using cells, blood vessels, proteins, DNA, RNA, metabolites, etc. in the body. It was defined as an index that can objectively measure and evaluate the disease progression and the drug's responsiveness to treatment methods. That is, in the case of a specific disease or cancer, it refers to a marker that can distinguish normal or pathological conditions, predict treatment response, and objectively measure it. Therefore, biomarkers should play a role in objectively measuring and evaluating the responsiveness of drugs to normal biological processes, disease progression, and treatment methods. A target marker that confirms the presence of a drug target according to the application, a diagnostic marker that diagnoses the presence or absence of a disease, a predictive marker that can distinguish a responder from a non-responder to a specific drug, and a surrogate marker that can monitor the effect of a drug treatment There are markers, prognostic biomarkers indicating the prognosis of a disease, and the like.

In the present invention, the NFX1 gene may consist of the nucleotide sequence represented by SEQ ID NO: 1, but is not limited thereto.

In the present invention, the protein encoded by the NFX1 gene may consist of the amino acid sequence represented by SEQ ID NO: 2, but is not limited thereto.

In the present invention, the MFN2 gene may consist of the nucleotide sequence represented by SEQ ID NO: 3, but is not limited thereto.

In the present invention, the protein encoded by the MFN2 gene may consist of an amino acid sequence represented by SEQ ID NO: 4, but is not limited thereto.

The biomarker composition of the present invention may include the NFX1 gene or a protein encoded by it, and preferably, the cancer prognosis predicted by the biomarker composition is the prognosis of the anticancer drug treatment, that is, the therapeutic responsiveness of the anticancer drug treatment. have.

In the present invention, the anticancer drug treatment may be performed using an OXPHOS inhibitor, irinotecan, or a combination thereof, but is not limited thereto.

In the present invention, the OXPHOS inhibitor is at least one selected from the group consisting of a ubiquinone reduction activity inhibitor, a mitochondrial electron transport chain I iinhibitor, or an iron chelator. It may include, but is not limited to.

In the present invention, the ubiquinone reducing activity inhibitor may be IACS-010759, but is not limited thereto. Here, the IACS-010759 is CAS Registration No. 1570496-34-2, and may be a compound represented by the following Chemical Formula 1:

[Formula 1]

In the present invention, the mitochondrial electron transport chain I inhibitor may be at least one selected from the group consisting of IM156, metformin, and rotenone, but is not limited thereto. Here, the IM156 is a derivative of metformin, also called "HL156", but N-(N-(4-(trifluoromethoxy)phenyl)carbamaimidoyl)pyrrolidine represented by the following Chemical Formula 2 -1-Carboximidamide (N-(N-(4-(trifluoromethoxy)phenyl)carbamimidoyl)pyrrolidine-1-carboximidamide). In this case, the acetate salt form of IM156 may be CAS registration number 1422365-94-3, the free base form may be CAS registration number 1422365-93-2, and the HCl salt form may be CAS registration number 1422365-52-3:

[Formula 2]

In the present invention, the iron chelator may inhibit mitochondrial respiration. The iron chelator may be VLX 600, but is not limited thereto. Here, the VLX 600 is CAS Registration No. 327031-55-0, and may be a compound represented by the following Chemical Formula 3:

[Formula 3]

The biomarker composition of the present invention may include the MFN2 gene or a protein encoded by it, and preferably, the cancer prognosis predicted by the biomarker composition may be the survival rate or survival period of cancer patients.

According to another embodiment of the present invention, the NFX1 gene or a protein encoded by it; and the MFN2 gene or protein encoded by it; It relates to a composition for predicting cancer prognosis, comprising an agent for measuring the expression level of at least one of them.

In the present invention, the agent for measuring the expression level of the protein includes at least one selected from the group consisting of an antibody, oligopeptide, ligand, PNA (peptide nucleic acid) and aptamer that specifically binds to the protein. can do.

In the present invention, the "antibody" refers to a substance that specifically binds to an antigen and causes an antigen-antibody reaction. For the purposes of the present invention, an antibody refers to an antibody that specifically binds to a protein encoded by each of the NFX1 or MFN2 genes. Antibodies of the present invention include polyclonal antibodies, monoclonal antibodies and recombinant antibodies. The antibody can be readily prepared using techniques well known in the art. For example, the polyclonal antibody can be produced by a method well known in the art, including the process of injecting an antigen of the protein into an animal and collecting blood from the animal to obtain a serum containing the antibody. Such polyclonal antibodies can be prepared from any animal such as goat, rabbit, sheep, monkey, horse, pig, cow, dog, and the like. In addition, monoclonal antibodies can be prepared using the hybridoma method well known in the art (see Kohler and Milstein (1976) European Journal of Immunology 6:511-519), or the phage antibody library technology (Clackson et al, Nature, 352). :624-628, 1991; Marks et al, J. Mol. Biol., 222:58, 1-597, 1991). The antibody prepared by the above method may be separated and purified using methods such as gel electrophoresis, dialysis, salt precipitation, ion exchange chromatography, and affinity chromatography. In addition, the antibodies of the present invention include functional fragments of antibody molecules as well as complete forms having two full-length light chains and two full-length heavy chains. A functional fragment of an antibody molecule means a fragment having at least an antigen-binding function, and includes Fab, F(ab'), F(ab')2 and Fv.

In the present invention, the "oligopeptide" is a peptide consisting of 2 to 20 amino acids and may include a dipeptide, a tripeptide, a tetrapeptide, and a pentapeptide, but is not limited thereto.

In the present invention, "PNA (Peptide Nucleic Acid)" refers to an artificially synthesized, DNA or RNA-like polymer, and was first introduced by Professors Nielsen, Egholm, Berg and Buchardt of the University of Copenhagen, Denmark in 1991. Whereas DNA has a phosphate-ribose sugar backbone, PNA has a repeated N-(2-aminoethyl)-glycine backbone linked by peptide bonds, which greatly increases binding strength and stability to DNA or RNA, resulting in molecular biology , diagnostic assays and antisense therapy. PNA is described in Nielsen PE, Egholm M, Berg RH, Buchardt O (December 1991). "Sequence-selective recognition of DNA by strand displacement with a thymine-substituted polyamide". Science 254 (5037): 1497-1500.

In the present invention, the "aptamer" is an oligonucleic acid or peptide molecule, and the general description of the aptamer is described in Bock LC et al., Nature 355(6360):5646(1992); Hoppe-Seyler F, Butz K "Peptide aptamers: powerful new tools for molecular medicine". J Mol Med. 78(8):42630(2000); Cohen BA, Colas P, Brent R. "An artificial cell-cycle inhibitor isolated from a combinatorial library". Proc Natl Acad Sci USA. 95(24): 142727 (1998).

In the present invention, the agent for measuring the expression level of the gene may include one or more selected from the group consisting of a primer, a probe, and an antisense nucleotide that specifically binds to the gene.

In the present invention, the "primer" is a fragment recognizing a target gene sequence, including a pair of forward and reverse primers, but preferably a primer pair that provides analysis results having specificity and sensitivity. When the nucleic acid sequence of the primer is a sequence that is inconsistent with a non-target sequence present in the sample, and thus amplifies only the target gene sequence containing the complementary primer binding site and does not cause non-specific amplification, high specificity can be conferred. .

In the present invention, the term "probe" refers to a substance capable of specifically binding to a target substance to be detected in a sample, and refers to a substance capable of specifically confirming the presence of a target substance in a sample through the binding. The type of probe is not limited as a material commonly used in the art, but preferably PNA (peptide nucleic acid), LNA (locked nucleic acid), peptide, polypeptide, protein, RNA or DNA, and most preferably It is PNA. More specifically, the probe is a biomaterial derived from or similar thereto, or manufactured in vitro, for example, enzymes, proteins, antibodies, microorganisms, animal and plant cells and organs, neurons, DNA, and It may be RNA, DNA includes cDNA, genomic DNA, and oligonucleotides, RNA includes genomic RNA, mRNA, and oligonucleotides, and examples of proteins include antibodies, antigens, enzymes, peptides, and the like.

In the present invention, the "LNA (Locked nucleic acids)" means a nucleic acid analog including a 2'-O, 4'-C methylene bridge [J Weiler, J Hunziker and J Hall Gene Therapy (2006) 13, 496.502) ]. LNA nucleosides include common nucleic acid bases in DNA and RNA, and can form base pairs according to Watson-Crick base pairing rules. However, due to the 'locking' of the molecule due to the methylene bridge, the LNA does not form the ideal shape in the Watson-Crick bond. When LNAs are incorporated into DNA or RNA oligonucleotides, LNAs can pair with complementary nucleotide chains more rapidly, increasing the stability of the double helix. In the present invention, the "antisense" means that the antisense oligomer is hybridized with a target sequence in RNA by Watson-Crick base pairing, and typically mRNA and RNA in the target sequence: A sequence of nucleotide bases allowing the formation of an oligomeric heteroduplex. and oligomers having an inter-subunit backbone. An oligomer may have exact sequence complementarity or approximate complementarity to a target sequence.

Since the information of the NFX1 gene and the MFN2 gene according to the present invention is known, those skilled in the art will be able to easily design a primer, probe or antisense nucleotide that specifically binds to the gene based on this.

The composition for prediction of the present invention may include an agent for measuring the expression level of the NFX1 gene or a protein encoded by it, and preferably, the predicted cancer prognosis is the prognosis of the anticancer drug treatment, that is, the therapeutic responsiveness of the anticancer drug treatment. can

In the present invention, the anticancer drug treatment may be performed using an OXPHOS inhibitor, irinotecan, or a combination thereof, but is not limited thereto.

In the present invention, the description of the OXPHOS inhibitor overlaps with that described above, and detailed description thereof will be omitted below to avoid excessive complexity of the specification.

The composition for prediction of the present invention may include an agent for measuring the expression level of the MFN2 gene or a protein encoded by it, and preferably, the predicted cancer prognosis may be the survival rate or survival period of cancer patients.

According to another embodiment of the present invention, it relates to a kit for predicting cancer prognosis comprising the composition of the present invention.

In the present invention, "kit" refers to a tool capable of evaluating the expression level of a biomarker by labeling a probe or antibody that specifically binds to a biomarker component with a detectable label. In addition to direct labeling of a detectable substance with respect to a probe or antibody by reaction with a substrate, it includes indirect labeling in which a color-generating label is conjugated by reactivity with another directly labeled reagent. It may include a chromogenic substrate solution, a washing solution, and other solutions to undergo a color reaction with the label, and may be prepared including reagent components used. In the present invention, the kit may be a kit including essential elements necessary for performing RT-PCR, and in addition to each primer pair specific for a marker gene, a test tube, reaction buffer, deoxynucleotides (dNTPs), Taq-polymerization enzymes, reverse transcriptase, DNase, RNase inhibitors, sterile water, and the like. In addition, the kit may be a kit for detecting a gene for prognostic diagnosis including essential elements necessary for performing a DNA chip. The DNA chip kit may include a substrate to which cDNA corresponding to a gene or fragment thereof is attached as a probe, and the substrate may include cDNA corresponding to a quantitative control gene or fragment thereof. The kit of the present invention is not limited thereto, as long as it is known in the art.

In the present invention, the kit may be an RT-PCR kit, a DNA chip kit, an ELISA kit, a protein chip kit, a rapid kit, or a multiple reaction monitoring (MRM) kit.

The kit of the present invention may further include one or more other component compositions, solutions or devices suitable for the assay method. For example, in the present invention, the kit may further include essential elements necessary for performing a reverse transcription polymerase reaction. The reverse transcription polymerase reaction kit includes a pair of primers specific for a gene encoding a marker protein. The primer is a nucleotide having a sequence specific to the nucleic acid sequence of the gene, and may have a length of about 7 bp to 50 bp, more preferably, a length of about 10 bp to 30 bp. It may also include a primer specific for the nucleic acid sequence of the control gene. Other Reverse Transcription Polymerase Reaction Kits include test tubes or other suitable containers, reaction buffers (varying pH and magnesium concentrations), deoxynucleotides (dNTPs), enzymes such as Taq-polymerase and reverse transcriptase, DNase, RNase inhibitor DEPC -Water (DEPC-water), sterile water, etc. may be included.

In addition, the kit of the present invention may include essential elements necessary for performing the DNA chip. The DNA chip kit may include a substrate to which cDNA or oligonucleotide corresponding to a gene or fragment thereof is attached, and reagents, agents, enzymes, etc. for preparing a fluorescently-labeled probe. The substrate may also contain cDNA or oligonucleotides corresponding to control genes or fragments thereof.

In addition, the kit of the present invention may include essential elements necessary for performing the ELISA. The ELISA kit contains an antibody specific for this protein. Antibodies are antibodies with high specificity and affinity for a marker protein and little cross-reactivity with other proteins, and are monoclonal antibodies, polyclonal antibodies, or recombinant antibodies. The ELISA kit may also include an antibody specific for a control protein. Other ELISA kits include reagents capable of detecting bound antibody, for example, labeled secondary antibodies, chromophores, enzymes (eg, conjugated with an antibody) and substrates thereof or capable of binding the antibody. other materials and the like.

As a fixture for antigen-antibody binding reaction in the kit of the present invention, a nitrocellulose membrane, a PVDF membrane, a well plate synthesized from polyvinyl resin or polystyrene resin, a glass slide glass, etc. may be used, but is not limited thereto.

In addition, in the kit of the present invention, the label of the secondary antibody is preferably a conventional color developer that performs a color reaction, and HRP (horseradish peroxidase), basic dephosphorylation enzyme (alkaline phosphatase), colloid gold (coloid gold), FITC (poly L-lysine-fluorescein isothiocyanate), RITC (rhodamine-B-isothiocyanate), such as a fluorescent substance (fluorescein) and a label such as a dye (dye) may be used, but is not limited thereto.

In addition, it is preferable to use the chromogenic substrate for inducing color development in the kit of the present invention according to the label that undergoes the color reaction, TMB (3,3',5,5'-tetramethyl bezidine), ABTS[2] ,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)], OPD (o-phenylenediamine), etc. can be used. In this case, the chromogenic substrate is more preferably provided in a dissolved state in a buffer solution (0.1 M NaAc, pH 5.5). A chromogenic substrate such as TMB is decomposed by HRP used as a label of the secondary antibody conjugate to generate chromogenic deposits, and the presence or absence of the marker proteins is detected by visually checking the degree of deposition of the chromogenic deposits.

The washing solution in the kit of the present invention preferably includes a phosphate buffer solution, NaCl and Tween 20, and a buffer solution (PBST) composed of 0.02 M phosphate buffer solution, 0.13 M NaCl, and 0.05% Tween 20 is more desirable. After the antigen-antibody binding reaction, the secondary antibody is reacted with the antigen-antibody conjugate, and an appropriate amount is added to the immobilizer and washed 3 to 6 times. As the reaction stop solution, a sulfuric acid solution (H ₂ SO ₄ ) may be preferably used.

According to another embodiment of the present invention, the NFX1 gene or a protein encoded by it in a biological sample isolated from a subject of interest; and the MFN2 gene or protein encoded by it; It relates to a method for providing information for predicting a cancer prognosis, comprising measuring the expression level of at least one of

In the present invention, the subject subject is an individual having cancer or a high probability of developing cancer, preferably human, rat, mouse, guinea pig, hamster, rabbit, monkey, dog, cat, cow, horse, pig, sheep and goat. It may be selected from the group consisting of, but is not limited thereto.

In the present invention, the biological sample refers to any material, biological fluid, tissue or cell obtained from or derived from an individual, and includes whole blood, leukocytes, and peripheral blood mononuclear cells. ), buffy coat, plasma, serum, sputum, tears, mucus, nasal washes, nasal aspirate, respiration (breath), urine, semen, saliva, peritoneal washings, ascites, cystic fluid, meningeal fluid, amniotic fluid , glandular fluid, pancreatic fluid, lymph fluid, pleural fluid, nipple aspirate, bronchial aspirate, synovial fluid, joint aspirate (joint aspirate), organ secretions (organ secretions), cells (cell), may be at least one selected from the group consisting of cell extract (cell extract) and cerebrospinal fluid (cerebrospinal fluid), preferably peripheral blood (peripheral blood) may be, but is not limited thereto.

In the present invention, the agent for measuring the expression level of the protein includes at least one selected from the group consisting of an antibody, oligopeptide, ligand, PNA (peptide nucleic acid) and aptamer that specifically binds to the protein. can do.

Measurement of the expression level of the protein in the present invention is a protein chip analysis, immunoassay, ligand binding assay, MALDI-TOF (Matrix Assisted Laser Desorption / Ionization Time of Flight Mass Spectrometry) analysis, SELDI-TOF (Sulface Enhanced Laser Desorption / Ionization Time of Flight Mass Spectrometry analysis, radioimmunoassay, radioimmunodiffusion method, octeroni immunodiffusion method, rocket immunoelectrophoresis, tissue immunostaining, complement fixation assay, two-dimensional electrophoresis analysis, liquid chromatography-mass spectrometry ( Liquid chromatography-Mass Spectrometry, LC-MS), LC-MS/MS (liquid chromatography-Mass Spectrometry/Mass Spectrometry), Western blotting, or ELISA (enzyme linked immunosorbent assay) may be performed.

In addition, in the present invention, the expression level of the protein may be measured by a multiple reaction monitoring (MRM) method.

In the present invention, in the method for monitoring multiple reactions, a synthetic peptide in which a specific amino acid constituting a target peptide is substituted with an isotope or E. coli beta galactosidase may be used as the internal standard material.

In the present invention, the agent for measuring the expression level of the gene may include one or more selected from the group consisting of primers, probes and antisense nucleotides that specifically bind to the gene.

In the present invention, the measurement of the expression level of the gene is reverse transcription polymerase reaction (RT-PCR), competitive reverse transcription polymerase reaction (Competitive RT-PCR), real-time reverse transcription polymerase reaction (Real-time RT-PCR), RNase protection assay (RPA; RNase protection assay), Northern blotting, or DNA chip.

In the present invention, the NFX1 gene or a protein encoded by it; and the MFN2 gene or a protein encoded by it; Measurement of the expression level of at least one of may be measured before, during, or after performing the anticancer drug treatment for the subject of interest.

The method for providing information of the present invention may include measuring the expression level of the NFX1 gene or a protein encoded by the biological sample in the biological sample, and preferably, the predicted cancer prognosis is the prognosis of anticancer drug treatment, that is, the prognosis of anticancer drug treatment. may be therapeutically responsive.

In the present invention, the anticancer drug treatment may be performed using an OXPHOS inhibitor, irinotecan, or a combination thereof, but is not limited thereto.

In the present invention, the description of the OXPHOS inhibitor overlaps with that described above, and detailed description thereof will be omitted below to avoid excessive complexity of the specification.

In the present invention, when the expression level of the measured NFX1 gene or a protein encoded by it is increased compared to the control group, it can be predicted that the target individual has a good prognosis, and preferably, the therapeutic responsiveness of the anticancer drug treatment is good or treatment It can be predicted that the prognosis will be good in the future. wherein the control is an expression level in a normal control group, an expression level in the corresponding cancer, an average value or median value thereof, or an expression level, an average value or median value thereof in the corresponding cancer of a patient with a relatively poor prognosis, or It may be the expression level measured with respect to the biological sample of the individual at a time point prior to performing the anticancer drug treatment of the same individual, but is not limited thereto.

In the present invention, when the expression level of the NFX1 gene or the protein encoded by the measured NFX1 gene is reduced compared to the control group, it can be predicted that the prognosis of the target individual is poor, preferably, the therapeutic responsiveness of the anticancer drug treatment is poor or after treatment The prognosis can be predicted to be poor. wherein the control is the expression level in the normal control, the expression level in the corresponding cancer, its mean or median value, or the expression level in the corresponding cancer of a patient with a relatively good prognosis, its mean or median value, or It may be the expression level measured with respect to the biological sample of the individual at a time point prior to performing the anticancer drug treatment of the same individual, but is not limited thereto.

The information providing method of the present invention may include measuring the expression level of the MFN2 gene or a protein encoded by it in the biological sample, and preferably, the predicted cancer prognosis may be the survival rate or survival period of cancer patients. .

In the present invention, when the expression level of the MFN2 gene or a protein encoded by the measured MFN2 gene is increased compared to the control group, it can be predicted that the prognosis of the target individual is poor, and preferably the survival rate of the target individual is low or survival The period can be expected to be short. wherein the control is the expression level in the normal control, the expression level in the corresponding cancer, its mean or median value, or the expression level in the corresponding cancer of a patient with a relatively good prognosis, its mean or median value, or It may be the expression level measured with respect to the biological sample of the individual at a time point prior to performing the anticancer drug treatment of the same individual, but is not limited thereto.

In the present invention, when the measured expression level of the MFN2 gene or the protein encoded by it is reduced compared to the control group, it can be predicted that the prognosis of the target individual is good, and preferably the survival rate of the target individual is high or survival The period can be expected to be long. wherein the control is an expression level in a normal control group, an expression level in the corresponding cancer, an average value or median value thereof, or an expression level, an average value or median value thereof in the corresponding cancer of a patient with a relatively poor prognosis, or It may be the expression level measured with respect to the biological sample of the individual at a time point prior to performing the anticancer drug treatment of the same individual, but is not limited thereto.

According to another embodiment of the present invention, a sample unit comprising a biological sample obtained from a target individual, an NFX1 gene or a protein encoded by the biological sample for the biological sample; And it may provide a device for predicting a cancer prognosis comprising a detection unit for detecting the expression level of the MFN2 gene or a protein encoded by the MFN2 gene.

In the present invention, the biological sample may be isolated from the subject before, during, or after the anticancer drug treatment is performed on the subject.

The description of the definition of the subject and the type of the biological sample of the present invention overlaps with those described above, and detailed description thereof will be omitted below to avoid excessive complexity of the specification.

In the present invention, in the detection unit, one selected from the group consisting of an antibody, oligopeptide, ligand, PNA (peptide nucleic acid) and aptamer that specifically binds to the protein as an agent for measuring the expression level of the protein It may include more than one species.

In the present invention, the detection of the protein in the detection unit is a protein chip analysis, immunoassay, ligand binding assay, MALDI-TOF (Matrix Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry) analysis, SELDI-TOF (Sulface Enhanced Laser Desorption/ Ionization Time of Flight Mass Spectrometry analysis, radioimmunoassay, radioimmunodiffusion method, octeroni immunodiffusion method, rocket immunoelectrophoresis, tissue immunostaining, complement fixation assay, two-dimensional electrophoresis analysis, liquid chromatography-mass spectrometry ( Liquid chromatography-Mass Spectrometry, LC-MS), LC-MS/MS (liquid chromatography-Mass Spectrometry/Mass Spectrometry), Western blotting, or ELISA (enzyme linked immunosorbent assay) may be performed.

Also, in the present invention, the detection of the protein by the detection unit may be performed by a multiple reaction monitoring (MRM) method.

In the present invention, the detection unit may include one or more selected from the group consisting of primers, probes and antisense nucleotides that specifically bind to the gene as an agent for measuring the expression level of the gene.

In the present invention, the detection of the gene in the detection unit is reverse transcription polymerase reaction (RT-PCR), competitive reverse transcription polymerase reaction (Competitive RT-PCR), real-time reverse transcription polymerase reaction (Real-time RT-PCR), RNase protection assay (RPA; RNase protection assay), Northern blotting, or DNA chip.

In the present invention, the detection unit can measure the expression level of the NFX1 gene or a protein encoded by the biological sample in the biological sample, and preferably, the cancer prognosis predicted by the device is the prognosis of the anticancer drug treatment, that is, the treatment of the anticancer drug treatment. may be reactive.

In the present invention, the anticancer drug treatment may be performed using an OXPHOS inhibitor, irinotecan, or a combination thereof, but is not limited thereto.

In the present invention, the description of the OXPHOS inhibitor overlaps with that described above, and detailed description thereof will be omitted below to avoid excessive complexity of the specification.

In the present invention, the detection unit may measure the expression level of the MFN2 gene or a protein encoded by the biological sample in the biological sample, and preferably, the cancer prognosis predicted by the device may be the survival rate or survival period of a cancer patient.

The device of the present invention may further include an operation unit for predicting a cancer prognosis by comparing the expression level obtained from the detection unit with the expression level in the control group.

In the present invention, when the expression level of the NFX1 gene or protein encoded by the NFX1 gene measured in the detection unit increases compared to the control unit, in the operation unit in the present invention, it can be predicted that the prognosis of the target individual is good, preferably the treatment of the anticancer drug It can be predicted that the responsiveness is good or the prognosis after treatment is good. wherein the control is an expression level in a normal control group, an expression level in the corresponding cancer, an average value or median value thereof, or an expression level, an average value or median value thereof in the corresponding cancer of a patient with a relatively poor prognosis, or It may be the expression level measured with respect to the biological sample of the individual at a time point prior to performing the anticancer drug treatment of the same individual, but is not limited thereto.

In the present invention, when the expression level of the NFX1 gene or the protein encoded by the detection unit is reduced compared to the control in the calculation unit, the prognosis of the target individual can be predicted to be good, and the prognosis of the target individual is poor It can be predicted, and preferably, it can be predicted that the treatment responsiveness of the anticancer drug treatment is poor or the prognosis after treatment is poor. wherein the control is the expression level in the normal control, the expression level in the corresponding cancer, its mean or median value, or the expression level in the corresponding cancer of a patient with a relatively good prognosis, its mean or median value, or It may be the expression level measured with respect to the biological sample of the individual at a time point prior to performing the anticancer drug treatment of the same individual, but is not limited thereto.

In the present invention, when the expression level of the MFN2 gene or the protein encoded by the detection unit is increased in the calculation unit in the detection unit compared to the control group, the prognosis of the target individual can be predicted to be poor, and preferably, the target individual's Low survival rates or short survival times can be predicted. wherein the control is the expression level in the normal control, the expression level in the corresponding cancer, its mean or median value, or the expression level in the corresponding cancer of a patient with a relatively good prognosis, its mean or median value, or It may be the expression level measured with respect to the biological sample of the individual at a time point prior to performing the anticancer drug treatment of the same individual, but is not limited thereto.

In the present invention, when the expression level of the MFN2 gene or a protein encoded by the detection unit is reduced in the calculation unit in the detection unit compared to the control group, it can be predicted that the target individual has a good prognosis, preferably the target individual High survival rates or long survival times can be predicted. wherein the control is an expression level in a normal control group, an expression level in the corresponding cancer, an average value or median value thereof, or an expression level, an average value or median value thereof in the corresponding cancer of a patient with a relatively poor prognosis, or It may be the expression level measured with respect to the biological sample of the individual at a time point prior to performing the anticancer drug treatment of the same individual, but is not limited thereto.

The present invention is a prognosis of a cancer patient, for example, the survival rate or survival period of a cancer patient, or a cancer patient It is possible to accurately predict the therapeutic response to an OXPHOS inhibitor and/or irinotecan, in particular, to establish an optimal treatment plan for cancer patients.

1 is a view illustrating gastric cancer cell lines classified according to genotypes, according to an embodiment of the present invention.
2 is a classification of cytotoxicity sensitivity of IM156 according to gastric cancer cell lines, according to an embodiment of the present invention.
3 is an analysis of marker candidates for IM156 treatment responsiveness, according to an embodiment of the present invention.
4 shows the results of analysis by applying to the Elastic-net regression model based on the viability value and RNA-seq for IM156 in 27 cancer cell lines according to an embodiment of the present invention.
5 shows the results of confirming the amount of mRNA of NFX1 according to the actual IM156 treatment responsiveness, according to an embodiment of the present invention.
6 shows the results of a Western blot assay for confirming the expression of genes for each cancer cell line, according to an embodiment of the present invention.
7 is a comparison of the effect difference of IM156 according to the amount of NFX1 expression, according to an embodiment of the present invention.
8 (a) to (c) show the results of immunostaining (Immunohistochemistry) according to an embodiment of the present invention.
9 shows the results of the correlation analysis between MFN2 gene expression and survival period according to an embodiment of the present invention.
10 shows the results of the correlation analysis between MFN2 gene expression and survival period according to an embodiment of the present invention.

Hereinafter, the present invention will be described in more detail through examples. These examples are only for illustrating the present invention in more detail, and it will be apparent to those of ordinary skill in the art that the scope of the present invention is not limited by these examples according to the gist of the present invention. .

Example

[Example 1] Correlation of NFX1 expression and prognosis in gastric cancer patients

1 shows gastric cancer cell lines classified according to genotype. As shown in FIG. 1 , as a result of confirming the mRNA levels of CDH1, VIM, and MLH1 genes by RNA-seq FPKM values in 29 gastric cancer cell lines, they could be divided into 4 subgroups. The low expression of CDH1 (E-cadherin: intestinal-like phenotype) and the high expression of VIM (Vimentin: EMT-like phenotype) were the EMT group of ACRG (published by Nature medicine 2015), and the stem-like phenotype of this example. like) was confirmed to be the same as the group. The three cell lines in which mRNA expression of MLH1 (MSS gene) was significantly lower than that of other groups were classified as MSI, the immune type in this example. Cell lines with high CDH1 expression and low VIM expression were classified as intestinal type. According to the state of P53 among the intestinal types, it was possible to again classify the P53 wild type and P53 mutant type. As such, by confirming mRNA expression, gastric cancer cell lines can be divided into a total of four subgroups, which is consistent with previously published papers.

2 is a classification of cytotoxicity sensitivity of IM156 according to gastric cancer cell lines, and FIG. 3 is an analysis of marker candidates for IM156 treatment responsiveness according to an embodiment of the present invention.

4 shows the results of analysis by applying to the Elastic-net regression model based on the viability value and RNA-seq for IM156 in 27 gastric cancer cell lines. As a result, it was confirmed that when the expression of NFX1 is increased, the sensitivity to IM156 is increased.

5 shows the results of confirming the amount of NFX1 mRNA according to the actual IM156 treatment responsiveness. As shown in FIG. 5 , as a result of confirming the mRNA amount of NFX1 with the FPKM value of RNA-seq in the cell group according to the sensitivity to IM156, the significance was confirmed by various statistical methods such as t-test, wilcox test, and ks test. As a result, it was confirmed that the expression of NFX1 was highly expressed in the cell line group sensitive to IM156.

6 shows the results of a Western blot assay for confirming the expression of genes for each gastric cancer cell line. As shown in FIG. 6 , it can be seen that, in MKN74, SNU620, and MKN28 of gastric cancer cell lines, NFX1 is expressed in a large number in the nucleus and cytoplasm, whereas in MKN45 and SNU1976, the expression level of NFX1 is relatively low.

7 shows the results of measuring the change in tumor volume after administering IM156 or a combination of IM156 and irinotecan to various gastric cancer cell lines with different NFX1 expression levels. As shown in FIG. 7 , when IM156 or a combination of IM156 and irinotecan was administered to MKN45 gastric cancer cell lines with low NFX1 expression levels, the diameter of the tumors increased, whereas IM156 or IM156 or MKN28 gastric cancer cell lines with high NFX1 expression levels increased. When the combination of IM156 and irinotecan was administered, it was found that the diameter of the tumor was significantly reduced compared to the control group.

6 and 7, in gastric cancer cell lines with a low NFX1 expression level, the therapeutic response of the combination of IM156 or IM156 and irinotecan was relatively low, whereas in gastric cancer cell lines with high NFX1 expression levels, IM156 or IM156 and irinotecan were used. It was found that the treatment responsiveness of the combination of

[Example 2] Correlation of MFN2 expression and prognosis in gastric cancer patients

To investigate the correlation between MFN2 expression and prognosis in gastric cancer clinical patients, tissue immunohistochemistry and tissue microarray technique (TMA) were analyzed. Specifically, in the tissue immunohistochemistry, normal gastric and gastric cancer tissues are fixed on a paraffin block, cut to a thickness of 5 μm, fixed on a slide, deparaffinized, dehydrated, reacted with MFN2 antibody, and reacted with a visualization reagent and reacted. After treatment with diaminobenzidine tetrahydrochloride (DAB-stained brown) as a dye source for the MFN2 antibody, it was counterstained with hematoxylin (nuclear stain, stained blue). The slide photos were analyzed by dividing them into 'strong' and 'weak' according to the staining degree of DAB, and the results are shown in (a) to (c) of FIG. 8. In addition, the tissue immunostaining Based on the results, the correlation between the level of MFN2 expression and the prognosis for 298 gastric cancer patients as a result of the tissue immunostaining was statistically analyzed through the tissue microarray technique, and the results are shown in FIG. 9 .

8 (a) to (c) and 9, the case in which MFN2, a mitochondrial fusion gene, is expressed in gastric cancer (Positive; Pos) compared to the case in which MFN2 is not expressed (Negative; Neg) in gastric cancer patients. was found to have a short survival period.

Through the above results, it can be seen that the prognosis is poor when MFN2 is expressed in gastric cancer patients.

[Example 3] Correlation of MFN2 prognosis in gastric cancer patients

In order to confirm the correlation between the gene expression of MFN2 related to energy metabolism and the patient's prognosis, gene expression analysis and correlation analysis were performed. Specifically, mRNA expression was measured using the affymetrix HG-U133A, HG-U133 Plus 2.0 and HG-U133A 2.0 platforms after extracting mRNA from 876 gastric cancer patients according to a protocol performed in a conventional procedure. After fixing the measured gene expression to one gene expression value, a process of analyzing the correlation with the survival period of each patient was additionally performed, and the results are shown in FIG. 10 .

As shown in FIG. 10 , when the expression of MFN2 was increased, it was confirmed that the patient's survival period was short, that is, the prognosis was poor.

As described above in detail a specific part of the present invention, for those of ordinary skill in the art, this specific description is only a preferred embodiment, and it is clear that the scope of the present invention is not limited thereto. Accordingly, the substantial scope of the present invention will be defined by the appended claims and their equivalents.

<110> Industry-Academic Cooperation Foundation, Yonsei University <120> Biomarkers for predicting prognosis of cancer <130> PDPB194288 <160> 4 <170> KoPatentIn 3.0 <210> 1 <211> 80738 <212> DNA <213> Homo sapiens <400> 1 aacgcgccac acagattcgg gctgcgcaac ctctgtggcc gtctacacgg cgcgcagatg 60 cgaatattct cgcggcgccg gaagtccggg gcacgtgacc tggtgacagt gctgacttgg 120 ctgtacagct cgatctaggt tctgcggcac gggatggcgg aggcgcctcc tgtctcaggt 180 attgtcccgg cccgagcggg actgggcccc tttcagggag cggaaattgg gtcagtgacg 240 aagttctagg gcctcagcca ctcatatcgc gagagtagca catgctaggg cgtaggatag 300 tggctcggaa gggccaagcc ccgcgcatcg tgcgtacgaa gatctgtgtc ttcttggact 360 agatggtgaa gcccagtagc tgggtttcgg aggagagagg acttgtcccg gcgctgcgag 420 tcgatgtggg tgccctgcct tcccacaggc tgtttctggg gcagcggcgc actgaccttc 480 gacaggcggg cgtcggccag gtcacagcca gtgacggtgg cgggctcttg acgtgtaggg 540 tgtggtgtgc tcataccgcc tggagtggtg gcgtgggcgc gagccgaatt ccagtagttt 600 agagtctcag cctgctgcga accccgtgtg actcagtctc agtctcctca tctgtcagtt 660 gggagtagat 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tatagtccca gctactcaag aggttgaact aggaggatcg 8280 cttgagccca gtgggtcaag gctgcagtaa gtagtggttg tgccactgca ctctagcctg 8340 ggtgacagaa taagaacctg tctcaaaaaa aaaaatatta ttaagctgct gtgtggagaa 8400 taggctctaa gaaggcaaca ggacaagtta ggaggctatt gcagttgttc aggtaggaga 8460 tgatggagac ttagactgga gtaatagtgt ggaaaaggta acaattggat agattttgga 8520 tgtatttgga aggaagattt tatgcctgag caacttaaaa gaatggaatt ggcatttact 8580 gaagtgtcaa agcctagtag agaaaaaggt gtttgtctgt aaaatgtttc agtttggaat 8640 aatttcagat ttatagaaaa gttgcaaaga tagttcaaag aattcccata tatctttcac 8700 ccaacttcct ctaatgttaa cgtcttacat aaccagactg cctttgttaa aactaagaaa 8760 ttagcactgg tataatcata ttaactaaac tacagatgtt attcagattt caccagtttt 8820 tctgccaatg tcctttttct gtttgaggac ccaatccagg ataccacgtt gcatttagtc 8880 atgtctcctg agtctcctac catctctgac agtttcttca tctttccttt ttggaaaaga 8940 tgtttctgct cttgttttat ttacttctta ttcagggaag atttttgttt aatttggtta 9000 aatttctgta atcccagtac tttgggaaac cgagtcaaga ggatcacctg agcccaggag 9060 ttcaagacca tcctgggcaa cagaggtaga ccccatctct acaaaaacta acaaaaaaat 9120 cagccaggta tagtggcaca cgcttgtggt cccagctact caggatgctg aggtgggagg 9180 atcgcttggg cccaggagtt tgaagatgca gtgagccacg atcatgccac tgcactcgag 9240 cctaaacttt ggtgacgcct gtaactttca cagaaagagg ttgtggaaca gactaaatat 9300 cactcattta ttcctttatc catgaattta acaaatattt aatggagcac ctagtatatg 9360 ccagacactg gaaatactac actcagcaag gtagacaaaa ctctcccttc tcctgagctt 9420 tcagtctcag ggtgttctct atttcttttg acattaaact gaacaatcag aaagtaaatc 9480 ttaggagagc caatattatg gaatattaca gtgtgcgctt ttgagtcaga tagtttgaca 9540 agtttgaatt ttgactttta aatttctagt tgtgtgacct tggataaatg atctaactac 9600 atgaagccag tttcttcatc tgtaaaatgg tacctatgtt atagcatttt tttttttttt 9660 tttttttttt ttgagacgga gtctcactct ggagtgcagt ggtgcaatct cggctcactg 9720 caacctccgc ccccgggttc aagagattct cctgcctcat cctccttgga ttactactgg 9780 gattacagcc acactccacc acgcccagct aatttttgta ttcttagtag agatggggtt 9840 tcaccatgtt ggccaggatg gtcttgatct cccgacctca tgatctgccc gccttggcct 9900 cccaaagtgc tggtattaca ggtgtaagcc accgtgtcca acctgttaca gcctttttga 9960 gaggactaaa taataatatg taatgtgctt gcaatgttgt atttaaggtt aattttattc 10020 ttttaactta tttagacctg aaagcaaata acaataatat tatgaaataa atgcagtgct 10080 aaaagtatgt actcaaaaaa gcatatacac acactcacac tcagttttga gagtatctgg 10140 ctgtattgaa atctagaaag actttgctgc aattgtgttt gaggaaaaaa atctggaagt 10200 gggtgaaggt attcaggaat agatctggga ggattattct gatactacac actggtctgt 10260 gtcactggac tttgagctcc tggagccagg gactgtaaca ccaccatggg gcttatggta 10320 ggcaccaaga atatctttgt taagtgaatg actaaagact ttgagggcaa cggctagagc 10380 catctcacca ctcatgggtt gttggaacct gcccagactt gggcttctta tagctgacac 10440 tacagcttca cgagcctctt tctaacctaa atccatggat ctgatagatt tagaggatcc 10500 tggaaaatag aaaatgcaaa gcaagatcct gtattgccca gtggatcctc aggctatgtt 10560 acaaggggca tttggctact ctgctgtttt cctgggaatg tttccttaat tgtgggaatc 10620 cccacctgag tgtctccttc ctaaatcctc ttcctcagca aattcctgcc cctttttggc 10680 ctcagtctga atagccagct gccttaccca gaatcagata tcccttaaaa tctctgtgtt 10740 tggattcttc tcttgagtga cttgctgatc cttggttgat taaagtgagg aatggttttt 10800 tgtgtttgag ttaatctttt ttgttatttt gttttttaaa caggttctct aattgaacaa 10860 ctaacaacag aaaaatacga gtgcatggtg tgctgtgaat tggttcgtgt cacggcccca 10920 gtgtggagtt gtcagagctg ttaccatgtg tttcatttga actgcataaa gaaatgggca 10980 aggtctccag catctcaagc aggtcaatta attctctctt ctgagtagtt attctcccct 11040 atttgatacg tttaagtatt attaagccca gctttaaaaa tacagtttaa tttctcttaa 11100 ctactttttc tgcagggaga gtatgttacc ttgatcatgt gtttcatatg accagaatta 11160 agttatagat cttaaataaa ccagctaaac aacaatatta aaatatattt taagatacga 11220 caaaatcagt cactcttatt gaaaatgatg gcttttactt attatttttt ctttgaccta 11280 ctgattttat ttcctgtgga tacataaaca gaagtgtgat tgcaggatca tatggtaatt 11340 ctatttttaa tttatcttct tctcattttt ggccaggcat gatggctcac gcctgtaatc 11400 ccagcgcttt gggaggccaa ggcaggcgga tcatctgaag tcagaagttc aagattagcc 11460 tggccaacat ggcaaaactc catctctact aaagatataa aaattagcca ggcgtggtgg 11520 cgggcacctg taatcccagc tactcgggag gctgaggcac aagaattgct tgaatccagg 11580 aggtggagat tgcagtgatc tgagattgca ccactgccct ccagcctggg tgacagagca 11640 agaccctgtc tcaaacaaac aaaaaaacat ttttttttca tttttaaatt tttactatgc 11700 atacgtaata gtttacatgt ttatggggta cacatgatat tttgatacaa gcgtacaatg 11760 tgtaatgatc aaatcagggt aattggcata tggggattcc aacacctcaa atatttatca 11820 tttctttgtg ttaggagcat cccatttcta ctcttctagt tattttgaaa tatataataa 11880 attgttgtta gccatagtgc tactaaacac tagatcttat tccttctatc taactgtatt 11940 tttttttttt tttttttttt gagacagagt cttactctgt tacccaggct ggagtccagt 12000 ggtgtgatca tgtctcactg cagccttgaa ttcctggact caaatgatcc tcctgcctca 12060 gactcccaag cagctgagac tacaggcaca tgccaccaca tccagctaat tttttttttt 12120 tttgagacgg tctgctgtgt tgcccagtct tgtcttgaac tcccaggctc aagcaattct 12180 gcatcagcct cctaaaatgc tgggattaga cacatgagcc accatgccta gctagtggtt 12240 tttttttgtt tgtttttgtt ttttttttga gagggagtct cactctgttg cccaggctgg 12300 agtgcagtgg cacgatctcg gctcactgca gcctccacct cccaggttca agtgattctt 12360 ctgcctcagc ctcctgagta gctgggatta caggcgcgca ccaccacgcc cagctaattt 12420 ttgcattttt agtagagatg atgtttcatc atgttagcca gggtggtctc aatctcctga 12480 ccttgtgatc cacccacctc tgcttcacaa agcgctggga ttataggcat gagccaccac 12540 acctggcctt tttttttttt tttttttaat gaaccccata tttagtggaa aatgctgtat 12600 tcaagctaag cagtcagtat tgtgaatttg ttccttaaat gtctgatatg cttaacattg 12660 aaattattac cactacttca atattgtgtc ttcctataga tttaaatttt tttaattaca 12720 tgtagctttt ggaagaaaat ttatttggcc tactcccatt tttcctgaca gatggccaga 12780 gtggttggag gtgccctgcc tgtcagaatg tttctgcaca tgttcctaat acctacactt 12840 gtttctgtgg taagtttgtt tatatacact ggagtctctt tactacata cattgtacct 12900 cagttcagga aaggtggtct gcggggcatg ttttagaagt tactaatgag acttcaaagc 12960 tctaggagtc tataagactg taattatgtg gtccatcaca cagctagagc cacagccttt 13020 gtgctcctgg caagctgttc tggagatggc caaccaggct catgctgctt ctgggctgta 13080 gtgcttgtct ccttggtctt gagaccaggc ctgaccatgt agcagagata ggtgtggacg 13140 tgggcttcat ctcaccccca gcagttacag ctttgatagt ccagatttga gcagtatgag 13200 taaataatag tgtttatttt aatcctggaa ataagtcatt ggtatttata atcagatttt 13260 ttataaacca gcattataaa ctctatcctg gcatcttccc tctttatgga atacttcttt 13320 ctgtttttga atatctcagt tcccccaagt attaggggaa aacttcttgt aacccagatg 13380 ctcattcttg ctgctagccc atttctgcat gttttttttc ctttcctgcc ttttaatgac 13440 taacttccct ctagttgttg atttttcaga ttttgtttct ttactgtctg tctccctccc 13500 tgaagcattg catttttgct tctgtcttgt tgcctcactg aaaccgtatt cagaaattac 13560 caatgggccg ggcttagtgg ctcacgcctg taatcccagc actttgggag gccaaggtag 13620 gtggatcacc tgagatcagg agttcgacac cagcctggcc aacatggtga aaccccgtct 13680 ctactaaaat aagttagctg ggcatagtgt tgcatgtctg taatcccagc tacttgggag 13740 gctgaggcag gagaatcgct tgaacctggg aggcagaggt tgcaatgatc caagatcatg 13800 ccattgcact ccagcctggg cgacagagtg agactccatc tcaaaaaaat aaataaataa 13860 aataaaaaca aagaaatcac caatgactcc tttccagcca tatctagggc tgattttata 13920 agctcataat ctttttggta gagtgatagc ccttgacacc tttgaccatt cccttcttga 13980 aataagtagc tcttcctgtt tgtttctgca tgctctcctt atttttctaa ccaatttgct 14040 catatttctc ctttcccatt acttagctag cagctttcac aaagagtcaa gtcatttacc 14100 atcaactctg aatcttctag atgcaatacc ttggtgaact ctttgacttc cccttctttt 14160 ctttcaattc ttaaccacag tatcatttaa gatctgaccc caaacaccct cttacaaagg 14220 aagctttcct ttgttattct ctgtctcact ttggcctctg ttttatttgg aattttccgg 14280 tcttacatgt tttattgaca gaatctcagc cttaatagat actaaattgg tattagatga 14340 gggaataaat attcttgaat atactaccag aaaggcagca tgaaactgga agtaagagat 14400 cagtggaaat ttgggcaaat aggacaatac caatacttaa taccttattt tgaaggatta 14460 cataaagtac cacaatggaa ttaaaaatct tgttaaagcc tctactggca aattagatga 14520 gagactctaa ggcagtataa gaatgtggag tgaaggtcaa gatgatctga aagtggtaaa 14580 atgatgtgag aatgcacata aaggccttat ttgcagatga ctttgcccca cactgttctt 14640 ccaggggacc attaatttaa catgttttta ttgagaactt attaatgttc aaggcactgt 14700 gaagaaacag aggcgaataa aacggttaac ttaccagagc ttacagtcta agagttaaaa 14760 cacaattagc ctgatactct gctagaagta caaagtacta tgggagatct ttggggtagt 14820 tacgacttac agctggatga ttaagagaag ccttcttgga ggaggtagaa ttgaactggc 14880 attgtaaact aggtaaaatc tttgtaggca gaaagatatg ttgaagggaa tagcataggc 14940 agttcaaaat caacagggtt tagggtatat tgggagaaga gtgattcatc cacttacaga 15000 gcccaggatc tatgatggta ggggagtggg agatgactgg aaaggaaggg ctgacccttt 15060 gataccagtc tcagatgttg ggacttcatt tggaaggctg tgggaagcca ctgagggcat 15120 caacatgatt ctttatggag attagtttgg tagcagtgag tagagtattt tggagcctaa 15180 ataatttggg acttataatc cagatgaaga gtagtacaag cctctaagga gtgtgataga 15240 aaaatatggt gttaggttga ttcctgacag tagtaggaat gggagtcacc caaagtttca 15300 tgcttttgga aatggtgata ccaaggaaaa gggcaggtca aaagaagaag gtctggtggg 15360 gacagtgact acagtttaga aatgttgagc tggaggagtg agcagaacat acagtgaaat 15420 atgtccaaca ggcaggtagc aatatttgag gaaagaccag gacttaagga tgagaatcac 15480 agctgagagg agtagttaag accctagcgg atgaaagggt acagagtgag aaaagggtgt 15540 aatgcagaac acttgcaggg gaaagaaaag caaaaaaccc caaaacatca tttggagtca 15600 gggacaaaag gtagagctct tgagagatgt tagaggaaaa ccagggcact gtattggagc 15660 aaaaaagcac agaggcaggg aggcttttgt gatagtctgg agatgaggtt ttggccagtt 15720 agagaggcag gaacaggaga gcgatgaggt gagagccact ttgtaggaaa aattgacaca 15780 acttggtgat taagtagaaa gaggtgtcag agatgactgt agtttttgag cgtgtgtgac 15840 tctgacagag ttgacgtcac tgacagaaat aggaaaccct gaaaagggag gagttataga 15900 gggaagatgt gaactttgac tgtggataaa gccagtttga ggtgactgtg aaaccccaag 15960 caagaatgtt cagcagacat ttggacatat gggtctgaaa ctgaagattc agggcaagag 16020 agaaaatgtg gatttgggga tcaatggcct ttaggtggat tgcacctctc aacatgaata 16080 aaatttctga aagagatagt atagagagaa aagcagaggc catggactgg ggatggctgc 16140 aatttagtgt gcaaattgcc caagtgagga tgaattcctc aagggcaagg aggaatcctg 16200 tgccttcgtt ttactcttcc ctgtctctgc tgagtccatg gaagatgtgc catgaagatt 16260 tgcagtgaca ttgattataa aagtataagg tcttttatgc cgactttata gttctggaat 16320 aggtgagttc ctgctactcg ggatttatgt cctttccatt tcttactgcc tccccccttt 16380 ccccacggtg ctcttctctt attccctctc tctgatcctt ccagcagcaa ttcgcatatc 16440 agttcagaag tccagttata gacgacttgt cttataggta tttatcttaa attaaacgtt 16500 tccattagtc aagatagtat tttcgtaagt aaaagtgact ttttatttat ctttcccagg 16560 taatccaaga tttaagattt atcatactca gtgaaacttt ttatgaatag cattatattt 16620 atctttttat tttaagatat gggataatac atttgttgaa ataataaaaa ccacactaaa 16680 gttgcatgct gcctagctat actttactgt ttcattaaag ttgagaatgt ggttgaagtt 16740 tgtaccattg actctctgat ctgatatgtt ctaggcaagg taaagaatcc tgagtggagc 16800 agaaatgaaa ttccacatag ctgtggtgag gtttgtagaa agaaacagcc tggccaggac 16860 tgcccacatt cctgtaacct gtaagttgga atgctaattc cgttgggatt gctggtggac 16920 atgctttgct ggtggctcta agtaaacata cctggttagc atgtaatggt ttgggatgaa 16980 gggtgatatg ggacctcagt aaatggtctc aaatcaccct gtgccaggca ctctacatca 17040 gttaacttgt tgaatcctta ccacagccca gtaatagata tttaccatct ctgctttaca 17100 gatgaggctc agaaaggcca agcctcttgc ccagcatcac agtggcaggg cgtggatggt 17160 ggagctaggg tttccaaagc cagtgctgct tccattacat tacactgctt gcttctaaga 17220 agaaatgtga ctcatatgtt ttatcccgca ttgatatcca ggtccaagag aatgtatggg 17280 agaaccttct gtctggggaa agacaattgt ttagggtccc ggtcggacag tatttccaag 17340 atgtcaagat gtcaatcttt ttctttcttt ctttcttttt tttttttttt ttttttgaga 17400 cagggtctcg ctttgtcacc caggctggag tgtgagtgtg gtggtgtgat cacagctcac 17460 tgcaaccgcc gcctcttgtg ctcaagccat cctcccacct catcctcctg agtagctggg 17520 actacaggcg tgtgccacca cgcctggcta atttttgtat gttttggtag aaatggggtt 17580 ttgccgtgtt gcccaggttg gtctcgaact cctgggctca agcaatctgc tctcctcagc 17640 ctcccaacat gccaggatta ctggtgtgag ccaccacacc cagccatagg atgtcagtct 17700 tttccctaca ataaatagcc agctgtagat tatatccata agccagacac ggtggctcat 17760 gcctataatc ccaacacgtt gggaagccaa ggcaggagga ttgcttgagg ccaggagttc 17820 tagaccagac taggcaacaa aatgagaccc cgtctctaca aaaaaattta aaattagcca 17880 gctgtggtgg cacacacctg tagtcccagc tacttgggag gctgagcaag gaggatcatt 17940 tgagtccagg agttcacggc tgcagtgagc tgtgattgca ctattgcact ccagcctggg 18000 caacaaagca agaccatgtc tctaaaaata ataaataaat aaataagtag actgtatgca 18060 gtcagcttct aaataccaaa aaatgcacag taatagaaaa acagttgatc agttacaaat 18120 aaatttctac aggccttaac ctcagacaaa ggtttaaatc ttaggttcaa gttacttaaa 18180 tagttatgta accttggaca agttattaat ttatctaagc ctgcatctgt aaaataataa 18240 tgatagtaat aataacactt atagggttct atgaaaatta aatgaattag tgcatctatg 18300 cggctcgata tagagtcttt ctggcaagtg atcaaaaaat gacaattctt acttttacta 18360 cccaaatcta agagtaaacc agacatcaga gtgttatctt ggtatttcta ctgcttggag 18420 gagggataat attggaccag ggccttgtga tcaaggcatg gtggtggcgc tggggaaatg 18480 tcagtggggg tagagctgtt ttaaactggg ctagacagca tggcctgtga tctgctgggc 18540 agtcagatac ctcattccct ttgactgctc gcataggtcc ttccttagga aggagttaag 18600 ctattcttgc cattagttgt cattttcagt aaattcagta ataagaagtt aatcctggct 18660 gggcgcggtg gctcatgcct gtaatcccag cactttggga ggccaaggcg ggtggatcac 18720 gaggccagga gatcgagacc atcctggcta agacggtgca accctgtctc tactaaaaat 18780 ataaaaaatt agccgggtgt ggtggtgggc acctgtagtc ccacctactg gagaggctga 18840 tgcaggagaa tggcgtgaac ccgggaggcg gaggttgcag tgagccgaga tcatgccact 18900 gcactccagc ctgggctaca gagcaagact ccgtctcaga gaaaaaaaaa aaaaacttaa 18960 tcctctgcat ggactatttt tccaaaccct tcatgactac cagagattaa aactgttgca 19020 gatcaagtga tcagcaccat gtctgaaaga aaagaacacc ttaaaacctg ctcctgctct 19080 tttgtggctc tggagctttc acacttgact tgggaacttt cttttacttt caggaccata 19140 gaagcccatt ccccagtagc acttttctgg tctccaagaa agctttgctt tctctcattt 19200 acacttgtta ggactcaaaa gtgacagaga cagggtctcg ctctgttgcc caggcttgag 19260 tgcagtggtg ctatcgtagc tcactgcacc cttgaactcc tgggctcaag cgatcctccc 19320 acctcagcct cccaaagtgc tgagagtaca ggcatgagcc accatgcctg gtgtgaacat 19380 caatattttt aaaagctctc caggcacttc caatatatag ccagtgttgt ggacctctgg 19440 tctaaaagtt aagaaaacct gagattgagg agattgagtc caggcttctg agatatttga 19500 cataacctag aatcacccac acctgacaag caatgaaaat tgtttcaccc gaaaggattg 19560 aggtatatat aaaatataac tgttgaataa tgatactcat ctttaaagaa acatgcttaa 1920 acttatgtct cctatgggta ttgtctatca aaatgagtca tcttagaaga ctagacactt 1980 gttcctggat tgttttcaat gtttaaactt tatttaagaa ttatcagagc ttgtttagga 197440 gtttcataga aactaaacat tccagccaga ccactttata agcacactaa tgttttacag 1800 caaattctgt tacccatctg attaattagt cttggctctg attggctttt gattcccaaa 19860 gggttgtaat ttgccaccta gaatataccc ctcctactcc aaaaacagtt gtcgtagaag 19920 ttcccaaaat gttttaagta gtggtatttc tgtaccactg tgtaatttgc caaagtgtct 19980 gctttgaaga gggcagtata tatttgaata tacaaattcc gtattgtaac ttttttctat 20040 tggtctctat aatatagctc ctaaaatagc atttttcaga gggctaattc tgacctatag 20100 catatcactc agcccagttc tttccatgac ccccttccct ttcattctct ccatgttact 20160 cactagcatg cctcccctca tgtcagcctt cttttcactg ttccattgtt tgcccagtgc 20220 ctgattatta cttcactagc tgatagtatg tctgtacttc ttacggacca ggtagatttg 20280 agttctatta ctttgcttag actctgatct tctacaggac cctattagcc attctgcctt 20340 ttacctgagt agcaaactat ttttatcccc acacactgct gtaagtgggc caagactgta 20400 tttgaaaatg tcatctttga ctctcagacc caaatataac cagtattttg acttgggatt 20460 tacttctgtg gttaaatagt ctgtggtgat aatgatgtcg attttttgca cataaaagta 20520 cagttgatat ctttgttcaa atagtttaat caactttaaa caaatacaaa gacatgtcat 20580 agtagatgta tttgttgcca tattcacaat aagacccagc tgggacagct ttgttcgggt 20640 ttggatacat ggctgtggtt tattcagtga aacctttctc tttcagtctc tgccatccag 20700 gaccctgccc accctgccct gcctttatga caaaaacatg tgaatgtgga cgaaccaggt 20760 aaagttaaaa ttacacccta aagaagacct cagttttcac atgcatgatt gacttgtgaa 20820 tttctgaaat aaaagaatag taggtagata caaataaatg gtaggcatga atagtacttt 20880 ttttttttca ggagtgaaag tttattaaaa agctttagaa caggaaggaa aggacagaag 20940 gaaagtacaa cttggaagag agccaagtgg gcaacttgag agaccaaatg tgccatggat 21000 agtacttttc tgtaactagt agttccctgt gctgcccatg accctatttt gtaatcttat 21060 aagtgattaa gaagttatat gcacaatgag attgtgagac tacccattat ttcgggtaaa 21120 ataccaaagt gacaaggatt tttttttttt tgagacggag tctcggtctg tcacccatgc 21180 tggagtgcag tggtgcgatc tcggctcact gcaacctctg ctgcctgggt tcaagcaatt 21240 ctcctgcctc agcctcccaa gtagctggga ttacaggcgc ctgccaccgc acctggctag 21300 tttttgtatt tttagtagag atgggatttc accatcttgg ccaggctggt cttgaactat 21360 tgacttcgtg atccacccgc ctcggcctcc caaagtgttg ggattacagg catgagctac 21420 cgcgctcggt cggcaaggat ttttttttta acctctctaa gtgggtgaca gaaccaggag 21480 ttgaacccag gcctaaggag gctgggacca gtttcctgtg cctagcacag tatctggcat 21540 ctaagagtct tttaatgaat attgttgaat gaaagaatag gtgactccag agcccaccct 21600 tgttccatta taatacaagg tctcctacat cattagtaca aggattactg atagctgtgg 21660 agtgtctctg aggcccagta agatcttaaa atgatttttg aaccacagga tgagatgaat 21720 catgcagatt ttcggtgagg tgcgtttgtt tagcctttac tgaacttatg tggacagcca 21780 tgtggcctga gattacatgt aagtgttttg actcagaaag gtcagatcct ggttctgtca 21840 cttactggtt gttgattctg ggcaagttgc ttggtctcac tgaacctcat ttttcttata 21900 aagtagaaat aatagtgtct acttcaaggg gttactgtta gccatggtgc taggcagaat 21960 ggcagcataa taggctagtg ttagccatta tgtcaagtac tgcaagctga tcatttatgt 22020 acataacagt gacttttaaa aacaaaatat ggagttgaga tgagagtcag ggatgaaata 22080 aaactcttct tgcaggagag tagactccaa gtgcttaacc attaccctag aaagagactt 22140 gaagacaatt gtctaaaagt actttttgcg gctgggcgca gtggcccacg cctgtaatcc 22200 cagcatttcg ggaggccgag gtgggtggat cacttgaggt taagagttcg agaccaggtg 22260 ggccaacatg gtgaaacccc gtctctacta aaaatacaaa aattggctgg gtgtggtgac 22320 acgtgcctgt agccccagct actggggagg ttgaggtagg agaatcgctt gaaaccagga 22380 ggtggaggtt gcagtgagcc gagatggctc cactgcactc cagcctgggc aacagagcaa 22440 gactctgtct caaataaaca acaacaaaaa aggactttct tatatccttc tctcagcaaa 22500 atcaggatgt ttattagaac aaaactagaa ttagcttatt tccctaccct gaagcaaagt 22560 cacatatagt atgttctaag tgctgggtct caggataatg ccattgtgct gttagtctga 22620 gtgtgtcaga tctgggtaca tgatagctgt cctcccgtct gtaaagtact gtggaagctc 22680 caggcagagc taggaccaga acccagatct tctaactcct caccccagca ttctttctcc 22740 ttacgcatgc tgcttcatgg aactcagaga tgaaatcaca taaagcaaat actcaggaat 22800 agccttcagt tcctcaccac tcagaaaata ggaaagaaag acaggatgga aaagaaaaag 22860 aaaaaggagg agaaagaaga aagagtgggc cgggcgtggt ggctcaagcc tgtaatccca 22920 acactttggg agcctgaggc aggaggatcg cttaagccta ggagtttaag attagtctgg 22980 gcaatatagt gagaccctgt ctctatttaa aaaaaaaaac aaaaacaaaa acagaagaag 23040 aaaaagagtg gagaaaggga gagtaattga gaaaataaga attggaggag ggagacatag 23100 gaaaaagtga gggaaatgaa gttagagaaa ggcttagtgg gttttgaggg gagagttagg 23160 ggccgcagtg ttagtttggt tgtccaacag gaacttttac actgatgctg tctttacatc 23220 tattgtcttt acaggcacac agttcgctgt ggtcaggctg tctcagtcca ctgttctaac 23280 ccatgtgaga atattttgaa ctgtggtcag caccagtgtg ctgagctgtg ccatgggggt 23340 cagtgccagc cttgccagat cattttgaac cagggtaagt ggtgggcaca ccagctagca 23400 atgcttgtgt tcttttctgg aaccttatta atgattgtct tgataaactt tgattggtgt 23460 cagactttta gtcacaaaga ccttgagtag agagaactgg agatacgatg cttagacggt 23520 ttttattatt ttacctgcat accagtatag gtcattgtcc tatataattt tttttttctt 23580 tttgagataa gtctcactct gccgcccagg ctggagtgca gtggcgtgat cctagctcac 23640 tgcaacctcc acctcccaag ttcaagcaat tctcctgtct cagcctcccg agtagctggg 23700 attacaggtg cctgctatca cgccttgcta atttttgtgt ttttagtaga gatagggttt 23760 cacaatgttg gccaggctgg tcttgaactc ctgacctcaa gtgataatgc ctgcctgggc 23820 ctcccaaagt gctgggatta caggcgtgag ccacagtgcc tggcctatct aatttaaata 23880 gtagatttta aaatgctgtt taaaagtaac tgcttgaaaa ggaccagatt attatggacc 23940 tagaaagacc aaaataatct ttttaaaaat gaactggccg ggcatagtgg ctcatgcctg 24000 taatcccagc actttgagag ggtgaggccg gtggatcact cgaggtcagc agattgagac 24060 cagcctggcc agcatggtga aaccctgtct ctactaaaaa tacaaaaatt aactgggcgt 24120 ggtggtgggc gcctgtaatc ccagctattc gggaggctga atcaagagaa tcacttgaac 24180 ccgggaggcg gaggttgcag tgagcagaga tggtgccact gcactctagc ctggatgaca 24240 gaatgaggct cagtctaaaa taataataat aaaatgaaca tatgtgaaat ctaagaacat 24300 ataaaactaa tttttcataa taaaaattag aaaatggttg cctagtggtg gaaattgggg 24360 gaagggacac aaattttctg ggttgataga aatgtttgat atcttgtttt gggtggtggt 24420 cacacagatg tatacaatta ccaaaagata tccgaaacca atcaaaactg aatatgtaaa 24480 atatttgtgt ttattatatg ctagctgtac atcaatggga aaaaaagtag aaagtatcta 24540 catgaataga aaaaaatttt ttaattgtga atataagttt atgtttttga tgttttcagt 24600 ttttctcttc ctctccatta ctgcaatcta gaaatggaac agaagaatct aattttatag 24660 ttggggcgtg ttggcgggcg ccggtaatcc caggtacttg gaggctgagg caggagaatc 24720 gcttgaacct gggaggcgga ggttgcagtg agctaagatc gtgccattgc actccagcct 24780 gggcaacaag agcggaactc tgtctcaaaa aaaaaaaaaa aaggaatcta gttttatttt 24840 ctcttagtgc aatgcaatta tccccttttt gctctccaga gtgaatattg tcagttaccc 24900 agtcgctgat taaacagcct gaaagctcac tgtattttta ggtaggctgt ttagtcatga 24960 tacaactcta atcgtcccag ttttttcttt tattgagcca aaatctgctt ccctgaaata 25020 tactctctcc ttcgtctgcc tttctctaca ttttatgttc ctcctgggtt gttcaaaatc 25080 agctgtcata tattctttcc ccagcgtttc ctttcaggcc taacagctgt gattgcttca 25140 tttcttccct gtgaaacatg ctcagagacc acacatcctc ttaaaagtgg ctagattggc 25200 ctgggcacgg tagctcatgc actctgggag gccagggcag gtggatcact tgagcctagg 25260 agtttgagac cagcctgggc aacatggcga aaaccagtct ctaacaacaa caaaaaaaaa 25320 aaattagcca gacatggtgg catgtatctg tagtcctagc tacttgggag gctgaggtgg 25380 aaggatggct tgagcccagg aggcggaggt tgcagtgagc tgagatcatg ctaccgcact 25440 ccagcctagg tgacagtacc cacctctgtc tcaaaaaaaa aaaagtggcc agattgttca 25500 caacaatgcc agtctttttg tgggatccac gaaaaccagt attgttcgaa actcggtgtt 25560 ctttgagtac attcttgaga tttttcactg agacggagga ctgattaaat gtgtcctgat 25620 tattttccag actaaaattt ctggtctgtt ctctgtggaa tagaacttca aggcttccat 25680 cacctattgt gaatcctgac cctgcctgtg ggtgctccct ccagttttat cccaggctga 25740 aaatgggata atatgttcca gcccctgttt ttaatagtct cttcaagaga atgaagagtt 25800 gcctgtgccc agtttgatta tccattatgt catatggatc tgtaattaac aggaagtgca 25860 gaaaagctct ctatcctggt acttaccaag cctttttttt ttttttggat ctcgttctgt 25920 catccaggcc agagtgcaga ttctcctgcc tcagcctccc aagtagctgg cactacagat 25980 gcacaccacc atgcctggct tcagctgttt ttagctcttg attcatattg ggcttttact 26040 cagctaaatc acccaagtct tttttgtaca aatttctgtg gatcccagtc tcttcctcct 26100 tgtttctgtt tagttgattt ttcaaaccca gttttgaaga ctttttgttt ttccacatta 26160 gttatctagc agaatatttt ggccatgtaa atagtttatc ttcttgaatt ataatcttct 26220 aggatccctt tcagccttat ctagagatta ataagcattc tttttgggtc tttattcata 26280 tgttgataaa atactaaatg ggttaaggct ggcaacaggg tcctttggtt ccttaatatt 26340 atttaaccag ctgtgactct acctaattct tctgtcacct gctcatattt ctgtctctcg 26400 tgcctaggag agctttttag tatttactca gattttttca tcaggaactc aattttttta 26460 ttgttacctg ttctgaccct tgattctttt gctcacagct ttctattcat tacctctgta 26520 aaaagccttt gttctgtttg agcagaaact tagcaaagaa aagcctacta gcaaaagctt 26580 ccagtttcca gggtttcttt accagttcat tgattctaca aatatttatt gtgttttctg 26640 aatctttatt aatttatcaa atcagtcttt attaaatgaa caaatgagtt ttagaacggg 26700 ggaaaaaatt gcatataggc cgggtgtagt ggctcacacc tgtaatccca gcactttagg 26760 aggccaggat gggctgatca cttgagccca ggagtttgag accagcttgg gcaacatggc 26820 aaaaccccat ctctacaaaa aatacaaaag ctagccaggt gtagtggcac gcacctgtac 26880 tcccagctac ttgaggggct gaagtgagaa cattgcttga gccccgggagt ttgaggctgc 26940 agtgagctgt gttcacatga ctgcactcca gcctgggtga caaagtgagg ccctgtctcc 27000 aaaaaaaaaa aaaaaagaaa gaaagaaaag aaaagaaatt gcacatgcac ttactggaag 27060 atgaaatgtg tattttaaaa ataaagagtc cttgaatctt aaattcagct aattaaaaaa 27120 aaaaaatcaa ggcaggcaca gtggctcaca cctgtaatcc cagcactttg ggaggtcgag 27180 gccgcagtaa gctgtgttca caccactgta ctccagctgg gcaacaaagt gagatcctgt 27240 ctttaaaaaa aaaaaaatct agagtcttaa ttatggccgg gcgtggtggc tcacgcctgt 27300 agtcccagcc ctttgggaag ctgaggcagg tggatcacct gagctcagga gttcaagacc 27360 agcctggcca acgtggtgaa actccatctc tcctaaaaat acagaaatta gctgggcgtg 27420 gtggcgggcc cctataatcc cagctacttg ggaggctgag gcaagagaat tgcatgaacc 27480 ggggaggtgg aggttgcagt gagccaagat catgccattg cactccagcc tgggcaagag 27540 tgagactctg tctcaaaaaa aaaaaaaaaa aaaaaaaaaa aatcttcatt atagtgaaac 27600 cttttggttc tgtttaatgt acgattttat tttatgagta ttatttttaa taccttatta 27660 gaaggtttct attttatttt tattgtatat gtgattttaa aaatgtgatg tttttataat 27720 actgcattta ttctgatcat tagatcttat actctcatgc caatttttaa atttcttttt 27780 ggcaacgggg acctttttac aagtgtttta tgtaaaacct gacataaagt atgaaacctg 27840 tgcttcttgg gttgaagtga ggatgggaaa tccagatgtc aactcctcca ccactactac 27900 cagcttagct tgtctatctc ctgtaacatc accagaggac ccgcaggagc ctcacagtga 27960 atggtgttca gccacggcct tatggaccag atagtgttct cttaatttag aggttgcttc 28020 actctgatgc tgttcttgga atgtgctgca gaggcactat gagatattaa tagtttaaca 28080 aggctttttt aatttcttcc tatggtaaag ctcttaggcg ggtcttagag ctgctgtatg 28140 aactatactc atttctgcat aggtctgatc agtactctat ttggcctccc agattacagg 28200 catgagcgac tgtgccaggc ccgcattttg tattttctta tagattattt gtgacatttt 28260 aagaacccat acatgttact aacgttttgt ttttgaaatt ttctcttcaa gtatgctatt 28320 gcggcagcac ctcccgagat gtgttatgtg gaaccgatgt aggaaagtct gatggatttg 28380 gggatttcag ctgtttaaag atatgtggca agtaaggttt tacgttttct tcagttgaac 28440 taaagctgca ctttatgcaa caattatgta atagtgtgtt ttcttaacag ggacttgaaa 28500 tgcggtaacc atacatgttc gcaagtgtgc caccctcagc cctgccagca atgcccacgg 28560 ctcccccagc tggtgcgctg ttgcccctgt ggccaaactc ctctcagcca attgctagaa 28620 cttggaagta gtagtcggaa aacatgcatg gaccctgtgc cttcatgtgg aaaagtgtgc 28680 ggcaagcctc tgccttgtgg ttccttaggt aactagtaag cgtaaagttg gctttaaaaa 28740 tattattgta aatggttggc agcagtgagt gtttaagcaa tgtagaagta agtgctagtt 28800 acaatatcag aggtttctaa atattatgat catatttatg taggtacgtt ttctttcctc 28860 ctcaacatat acttgtgaaa acccatacat ttgcccaaat gatctctctg attcaggtat 28920 tttattgtta actctggaca actgtggtga gttactgttc cataggggtt atgatttctc 28980 ttaggagcct gtactgtaat gaacactata tgtatttata ccaatccagg gttcagcaag 29040 tgttgaaaac ccttttcaca taataggaac atgctaaaat aattgtgggt gagacagagt 29100 ctggtttgga gacagagtct cgctctgtcg ccccagctcg agtgcagtgg taggatctca 29160 gctcactgca gcctctgact cctgggctca aatgattctt gtgtctcagc ctcccaagta 29220 gctgggacta caggcgcaca ccaccacgcc cggctaattt ttgtattttt aataagagacg 29280 gggtttcacc atgttgacca ggctggtctc gaactcctgg caccaagtga tccacccgcc 29340 ttgggttccc aaagtgctgg gattacaggt gtaagccact gtacctggcc aaagaactaa 29400 aattgtaagt acaatagaac ctcagccatt tagatcattc aaggaatgtc agcctctgta 29460 gctaagtttc ccatctagct gaggatacga ggatagcacc tattgaacat cattttggat 29520 gaatattttt cttttctttt cttttctttt tttttttttt gagacagagt ctcgctctgt 29580 tgcccaggct agagtgcagt ggcgtgattt tggctcactg caacctctac ctcctgggtt 29640 caagcaattc tcctgccttg gcctcccgag tagctgggat tacaggcacc tgcaatcatg 29700 cccagctaat ttttttttgt atttttgtag agacggggtt tcaccatgtt ggccaagctg 29760 gtcttgaact cctgaccttg ggtgatctgc ccgccttggc ctcccaaagt gctgggatta 29820 taggtgtgag ccaccatgcc cggctggatg aatatttttc taaaatttac atttcatgtt 29880 taaacagagt ttactgaagg tgttgtaatt tttctctcat tgacccagag tcatcatttt 29940 tatgaactcc agttactgta ttacactgaa tgcagtggtg cccttggcct gctgaggttt 30000 ctcagactga tgccccatac caatgagcag tttgaattgt tgtatctgct tgttgaagct 30060 ttttgttgtt tatattctta tcagtgcttt tagtatgctt gcctgtctac ttttagtaat 30120 tccccctttc ttctgatttg tggcttctga ttacttgaat atagaaacca gaaaaagctc 30180 attggaatta gatacaaagt aatactatac tgagcctatc tgactcagtc ttttgggaat 30240 gtatgtctgc agttttgcaa tgacttggca acttctctcc tgagtgggtg ttgaaaggcc 30300 atgcatttag aaaaaaatgg aaagtaggca actgagattg aataggaaaa ttatttctaa 30360 aactaaccat gtatcttgtg aggtaaagct agaatcttta ggtttggttt ccatttcatc 30420 aaaaggagag agttcacatt tctttcaagt gccagcgtga atcagtggga acaccctact 30480 taatcctttt tctcaatctg accttaaaat tgtgtataga ggagaagtgc tgtcttcttg 30540 tattttaata tacattccag ctgcattagg aaagccagtc atctgttggt tctctgcttg 30600 ccatgaaaat gtgtttatga tatggagatc aaatgactag taaatcaaat cccctttcgg 30660 aaagcacatg agtcagtttt ataagaattt cacatataat gaccagaaat cctaccctgg 30720 cactcatgta tcctgttggt gatgagagaa gaaggaaatg tgaaatgggt aatgcaaagg 30780 gacttgagca agagaagggt agtaactggc agtaacagac cttgggatgg gacaagacca 30840 ggttaaggga taagagggta cctgctatac ctcagcaggt atcatggaaa acctttaagg 30900 aaataggctg agttgactga gaagctcaga gacaagcgtg tggctagcga taacaatgac 30960 tgtcaggttt gttcagacct agcttatcta tagaaagctc gcttaggtct tttgtatctc 31020 actcagtagg aaatttccat cccagagaca cagcttcagt ataagagact ggatagagtt 31080 ctactttaaa gatggtagca tgaagagctc ccatagatct gcttcccagt aaagcaaaca 31140 aagctaggga aaactataaa aaagcaacca tcaaggctgg gcatggtggc tcatacctat 31200 aatctcagca ctttgggagg ccaaggtggg agaactgctt gagtccagga ctttgagacc 31260 agcctgggca acatggcaag accctgtatg taccaaaaaa cattttttat ttacctgggt 31320 atggcggcac gtgcctgtgg tcccagctac ttgggaggct gaggcaaaag gatcatttga 31380 ccctgggatg ttgaggctgc agtgagccat gaatgcagca ctgcactcca gctaggctga 31440 cagagtgaga ccctgtctca aaaaaaaaaa aaaaaatcaa ccatttaaag tttctggagg 31500 ctgggtgcag tggttcacat ctgtaatccc ccacactttg ggatgctgag gtgggcagat 31560 cacttgaggt caggagttca ggactagcct ggccaacatg atgaaacccc gtctctacta 31620 aaaatacaaa aattagccag gcatggtggc acatgcctgc aatcccagct actcgggagg 31680 ctgaggcagg ggaattgctt gaacctgggg ggcagtagtt gcagtgaact cagatcatgc 31740 cactgcactc cagcctaggt gacagagtga gactccatct cgaaaaaaaa aaaaaaaagt 31800 ctctagaaat tgccctaaga gcatacctca aatgaagaca tatttattca acaaaatctt 31860 ctaaaactct ggtaaacaac agcaagagtc tgtggcattg agccctagct agttccttcc 31920 tcctcccctc agctcagctt gacagatctc cactcaggat aagtgtggac aagaagatgg 31980 ggcttcttct cccctcagct cccagtcagg tttaccatat ctcactggga aggccacatt 32040 gtcaacattt ctctacccct ccaactccaa gttgaagagg ctgaaattct ggtgagtgca 32100 gctgagcagt tagggctccc tttctccacc aagctcccac ttacagaatg gaggcttcat 32160 cccatttgca gcagaatact ggagccctaa tcgtcctcac cccagctcac tcgtagggca 32220 gaggttccac tgagagaagc aagccaagaa ggcaagaggc tactatccca cccagcaccc 32280 agagtagtgc ctcagagacc tttcccaggg cgagagacag ttcataacaa aagagagctc 32340 cgaggtactc tccaaaggaa ctgactttat ttgaaacagt gtggggaagt acaggcctaa 32400 ggacacttta gaaaatgata acatacctta aagaacagta actaaactgt aggccatcta 32460 gttcatcaga gagaatcagg gaaaggtagc taagaggagc cctcttcagg tcagaacaaa 32520 tctcaaagac ttacagcaaa gactattcct tcccaaattt aattggatca gactgctgag 32580 caattgatgc cctggaacat tgtcaaaaac aatagaactg caattaatgg atccttgaga 32640 ctgagcgtaa tccaaataag gcacacagtt taacagagag atcgggaatc aagacaaaga 32700 gagccctgct tgaaacgact gtcattccag ggtcagtttg tacccctaag gttgtgccat 32760 ccaaagagtg acaccagcgg ctacatactg tagggaaaat agacttcact gaaatagtct 32820 agccaagtca caaaacaaat aaataagcaa ccacaacaaa aacaagctgt ggagagggga 32880 agcagtatcc agagttgcta tatagctaaa atgtctagtt tccaagaaaa agtatgaaat 32940 gtgcaaagaa acagggaaaa aaacaggcaa cagaaactgc cttgtgagag tgaccagatg 33000 tcagatttca caaagactgt aggataatca taataaatat gttcaaagaa ctgggccagg 33060 catggtggct cacgcctgta atcccagcac tttgggaggc tgaggcagga ggatcacgag 33120 gtcaggagtt caagaccagc ctggccgaca tggtgaaacc ccatctctac taaaaataca 33180 aaaattagct gggcatgttg gtatgcgcct gtggtcccag ctgctccggc ggctgaggca 33240 ggagaatccc ttgaacccag gaggcagagg ttgcagtgag ccgcgattgc gccactgcac 33300 tacagcctgg gcaacagagc aagacttcat ctcaaaaaaa aaaacaaaca aaaaaaaaca 33360 aataaataaa tatcttcaaa gaattgaagg gaaaccatac ttaaagaggt aaagaaagat 33420 gtgaagatga tgtcttatca aatagagaat attgataaag agatagaaac tagttttaaa 33480 agaggcccaa atggaaattc tgaagtttaa aagtaggata accgaaatgc aaaattcatt 33540 aggctgggta ccgtggctta tgcctgaatc tcagtgcttt gagagatgga ggcaggagaa 33600 ttgcttaagc ccaggagttc aagaccagcc tgagcacgta gtgagaccct atctctacaa 33660 aaaattttaa aaacttaggc caggcacagt ggctcatgcc tgtaatccca gcactttggg 33720 aggctgaggc aggcagatca cttgggatca ggagtttgag atcagcctgg ccaacatggt 33780 gaaatctcgt ctctactaaa aatataaaaa ttagctgggt gtggcggcac acgcctgtaa 33840 tcctggctat ttggaaggct aaggcaggag aatcgcttga acccagtagg cggaggttgc 33900 agtgagccga gatcgcacca ctgtactcca gcctggacga gggagtaaga ctccatctca 33960 aaaaaaataa taataattaa atttagctgg gcatggtggc acacacctgt gatcccagct 34020 actcaggagg ctgaggtggg aagattgctt gagtccaaga ggttgaggtt gcagcaagcc 34080 atgatcatgc cactgcgctt caggtgggca agaaagcaag accctttctc aaaggaagaa 34140 gaaaacttca ttaaaggggc tcaatagtag atttgagctg gcagaagaaa cgatcagtga 34200 acatgaaaat agattgaaag agatttggtc aggcacagtg gctcatgcct gtaatcccag 34260 cattttggga ggctgaggca ggcgaatcac gaggtcagga gatcgagacc atcctggcta 34320 acatggtgaa accccgtctc tactatatac aaaaaaaaaa ttagccaggt gtggtggcgg 34380 gcacctgtag tcccagctac tcgggaggct gaggcaggag aatggtgtga acctgggagg 34440 cggaggttgc agtgagctga gattgtgcca ctgcactcca gcctgggcaa cagagtgaga 34500 ttccatctca aaaaaaaaaa aaaagaaaga gattatataa cccaacggac agagagaaaa 34560 tataatgaag agaaatgaac aaagcctcag aaatgtggga cacctttaac tgtatcagtg 34620 tacatgtaat tggaatatga ggaggagagt agaaagaagt ggaaaaatat ttaatgaaat 34680 aatagataaa aactttcaaa tgtattgaaa aacactacac atccaggaaa ctcagtaaac 34740 tccaggtagg aaagagatcc acatctagac atataataaa aatgctaaaa gttaatgata 34800 aggagaaagt cttgaaagca gcaagagaaa aatgacttat cacatacaag ggaacctctg 34860 taagcttaac agtggacttc tcatcagaaa caatggagaa cacatcacaa ccagaaggca 34920 gtaggatgac atattcaaag tgctgaaaga aaactggcaa ctggccgggc gcggtggctc 34980 acgcctgtaa tcccagcact ttgagaggcc gaggcgggtg gatcacgagg tcaggatatt 35040 gagaccatcc tggctaacac ggtgaaaccc tgtctctact aaaaaataca aaaaattagc 35100 cgggcatggt ggcgggcacc tgtagtccca gctactcggg atgctgaggc aggagaatgg 35160 catgaacccg ggaggcgtag cttgcagtga gccgagattg cgccactgca ctccagcctg 35220 ggcaacagag agagactctg tctcaaaaaa agaaaaagaa aaaaagaaaa ctgtcaacca 35280 agaatcttac atctcatgaa actaacttta gaaaatgaag ataaaaacaa aatttattgc 35340 tagcaggcag acttgcctta cagtaagtac taaaagaaat tcttcaggct ataagcaagt 35400 aaacccaaat agaatttcaa atccacatga aaagacaaag agcactagta aagatgatta 35460 tgtaatcata aaaggcagta taaatgcata tttcttcttc tcaactgatt taaaaaacaa 35520 ctgtataaaa taatatgtat aaaattatat tttaggccta taacctgtag aagtatattt 35580 gataattata gcataaagga gatagatggg atcaaagttg tgttagaata agaaaatgat 35640 accagatcac aactcaaacc tacaggaaca aatgagggaa gccataagtg gtaaattttg 35700 tataagaggg gggctaggcg cagtgactca cacctgtaat cccagctctt tgggaggcca 35760 aggtgggcag atcacttgat gtcaggagtt tgagaccagc ctgaccaaca tggtgaaact 35820 ccgtttctac taaaaataca aaaattagtg tggtgtggtg gcatatgcct gtgatcccag 35880 ctactcagga ggctgaggca caaaaattgt ttgaattcag gaggcagagg ctgcagtgag 35940 ccaagatcat gccactgcac ttcagcctgg gcaacagagc aagcctctgt ctttaaaaaa 36000 aaaaaaaaaa aaaaaaaagc tgggcactgt agctctcacc tgtaatccca gctactcagg 36060 aggctgaggt ggaaggatca cttgaggcta ggagttcaag gccagcctgg gcaacgtagt 36120 gagatttaat ccctcaaaga aatttgtaaa attacccaga catggtggta tgtgcctgta 36180 gtcccaccta ctcaagaggc taaagtgggg gatcacttga ggccaagagt tcaagactgc 36240 agtcagctat gatcatgcca ctgcattcca gcctgagtga caagagtggg accctgtctc 36300 caaaaattaa taaagctata aatatata tttgttatct ttttcagctt ctttaataga 36360 tataacatta tataaagttc taacaaagta gtattgggtt tataacatat agatgtgata 36420 tgtataacaa taactgcaca aaaggggaga agaatagagc tgtgtaaggg taaaccttct 36480 gtatcttact ggagttagta taaatctgaa gtagattctg ataagatgta tatggtaagc 36540 cctagaggga ccattaagaa aataactcaa aacatttagt gaaaaatata attaagggaa 36600 ttacactata aaaaatattg acaaaatgca aatgaaaata ggaaaggagt attagaataa 36660 caaaaaagac atgagactta cagaaaacta aaaaagacga acataaatcc agctataaca 36720 gtaacattaa atgtgaatgg attgagcaaa agcagatatt tttgagtttt gatttattta 36780 ttttttatag aggtggaatc tcactctatt tcctgggctg atcccgaact cctaggctca 36840 agcagtcctc ccaccttggc ttctcaaagt gctgggatta caggcatgag ccactgcacc 36900 cagccaaaag cagaaatttt ttagactgga tttaaaaaac aagatccaac tatatgctct 36960 cttgagatgg agtcttgctc tgttgcccag gctggagtgc agtggcatga tcttggctca 37020 ctgcaacctc cgcctcccag gttcaagcga ttctcctgcc tcagcctcct gagtcgctgg 37080 gattacaggt gggcgccacc acgcctggct aatttttttg tatttttagt agagacaggg 37140 tttcaccatg ttggtcaggc tggtctcaaa ctcctgacct catgatctgc ccacctcggt 37200 ctcccaaagt gctgggatta caggcgtgag ccaccgcacc aggccatgga gacaatcttt 37260 atagtcaaag atacaatgag gctgaaagtt aaagaataga aaaagtacac catgtaaaca 37320 gtagccataa gagaccccag gtgatcccca tagggtcctg gaaacaggac ttgtcctaaa 37380 cctagtttag cagcatggat gcaggcacca ccagacattc acagagctct agtcagtcag 37440 actgtggatc atggcttctg attatttata atccccagat gactttcaga gtcgacagca 37500 ttccactaaa gtcatccatg gctacagttt tagggagatt caagtctaca ctggagatgt 37560 ggggcctgag gaaaaagaag atttattctg tgtttttgtt atttgttttg aaacagggtc 37620 ttgctctgtc acctagactg gagttcagtg gtgcgatcac agttcactgt agcctcaacg 37680 tcccatgctc aggagatttt cccacctcag actcctgagt agctgggact gtaggcctgc 37740 atcaccactc ctggctaatt tcttgttttt gtgctttttt tttttttggt agacacaggg 37800 tttcaccatg ttcccaggtg atctcgagct cctggggtca agcgatcctc ccacctcagc 37860 ctctcaaagt gctggaatta taggcatgag ccaccacact tggccttttt ttgacttagg 37920 caaatcgtcg tatactttaa agcatattcc agtgtctaag ctgggccctg aagttgtcag 37980 aaaaggccca gtctgccaga tgaagggaag atagggaaga agcattgact gcatacttcc 38040 ctcaactctg attctggagt tgcagggcat ggaggggaag accagtgtgg ctagcaaatt 38100 tgggagtatg aaaatgagta gttgcagttt tcatttccag ctcttttcgt ttttattaaa 38160 gatttcattc atacctgtga aaagctctgc catgaaggag actgtggacc atgctctcgc 38220 acatcagtta tttcctgcag atgctctttc agaacaaagg taaatcctaa acaatgctag 38280 agttgttgcc atcaatgttt tctttaaaat ggtgattatg ggaggggagg aatcaaaata 38340 acctcagtag attaggtatg ggaagtggtt gtggcactca aggtccagag gcctatagtg 38400 ggataaggca aatatacaga tcactctacc aaatacaggt gtgatagtct gaaaagggga 38460 acagtagcac tgttgcaagg agtcagccaa ctaagaagtt agaggaaaca cagtccatat 38520 gaccacccac ttgtggcacc aacttttgat accaattgaa agttcagtgg tccctaagac 38580 cacctgcgag gcttgataat ttgataaaag gactcaccga acttgctggt ggttacactc 38640 atggttagag ctttactaca aggaggttac agttttatta caaggaaaag gatacagatt 38700 aaatcagcca agggaagaag tatgtaggac agagtccaaa aaaataccaa aggtggaact 38760 tcatttgtcc tttccctgtg gagtcaggaa agtattactc tctccgcttc agtgtgtgcc 38820 agcatacatg gaatattgcc aaccaggaaa gttcacccag acctgtgtgt tcagttttta 38880 ttggtgctct attgtgcaat catagttgac tgcccatgtg gctaatctca gtctccagtc 38940 tctcccgagg tgtaccgata ccatgtgacc caaaacccct gccctaaaac tatccagtgt 39000 ggccagcctg ctcctaaatc acgtaattgg actatccagt gactcaagac tccaggcaaa 39060 caatgacact tatatcaggc atggcattcc actaggctta gagattacct ctcagaagcc 39120 aagcacaaag gccagactct ctttaagtaa ggtttaattc ttcactgtac atctgttttg 39180 tcagtttcta ctttctggtg aaagcctgga tggtttttat ttttcttttt ttttttttaa 39240 ttttatttat ttattttgag acagagtctc actccatcac ccaggatgga gtgcagaggc 39300 gcagtcttgg ctcactgcag cctccacctc ccagattcaa gtgattctta tgccttagcc 39360 tctggagtag ctggaattac aggtgcatgc catcacaccc ggctaatttt tgtattttta 39420 gtagagacgg gattttgcca tgttggccag gctggtctca aactcctgac ctcaagtgat 39480 cctcctgcct cagcctccca aagtgctggg attacaggtg tgcaccacca ctcctggcta 39540 atttttgtat ttttagtaga ggtggggttt caccatgttg gccaggctgg tcttgaactc 39600 ctgacctcag gtaatctacc cgcctcagcc tcccaaaatg ctgggattac agacgtgagc 39660 cactgtgccc agcctacctt ggatagtttt catcagactt tatgttataa aaagtcttcg 39720 ggtgattcag cttggggaac acactgagtg ttgatttatc ctgctctttg acctacttag 39780 tcattttagt gtatatcaac atttgtccca gtagagtaca agccagggtg gagacagaat 39840 tgtgaaccgt tggttctgtt aaacacaatg cagggtctgg cagcatctga cttgcgaata 39900 attctgagga tatagtccat aaatgggaag ctagtttaat aggaaaactt cagaaaaaag 39960 ggaaaagtag tataatttac atgttacaaa agttgtgtaa tcatatatgg gttttttcct 40020 gaaagaaatt acaatataca taggataaca gaggcatttt tgaatgtcag gtatgggacc 40080 acttttgtac tttgtaaata ctgtattaaa gactgagatg ctgtgatttg gcaatcataa 40140 atttagtaat gcccagagat gctctttgtg atctgtgtga ctgaaaattg taagcagggt 40200 tgggacatgt aagctggaag tggctgctgt cagttttctt cattctccca caatccctct 40260 ttctatctct acgttttctt gcttctcatt gactctttgt cctcactccc tctaatagtg 40320 gactccatgg cagtgagtgt attcttgaaa agctatgtgt aagttgaatt tttgcaagta 40380 gaagtcctta aattgcagga ggagctcact gtcaatacaa actctgttaa acctgtttga 40440 caaagtaaaa aaaaaactct tcaaaaagct gataattggc tgggtgtggt ggctcacgcc 40500 tgtaatccca gcactttggg aggctgaggt gggcggatca tgaggtcagg agattgagac 40560 catggtgaaa ccccgtctct actaaaaata caaaaaatta gccaggcacg gtggtgggcg 40620 cctgtagtcc cagctactcg ggaggctgag gcaggagaat ggtgtgaacc caggaagtgg 40680 agcttgcagt gaaccgagat tgcaccactg cgctccagcc agggcaacag agcaagactg 40740 tctcaaaaaa aaaataaaaa aaataagctg ataattatca cctcattgtt taactagatt 40800 tttcatgaat atggattttt catgacatgg ttttggtaat ggaatgggct tttccacaga 40860 agtgagtttt ctagataaat aaaacatttc ttaaaacttc aaaggttgtt ttcatttcat 40920 cactttggtg ggattctgat aaatgtatgc tgtgtctatg tcttcataaa gcatgctata 40980 tttaagtctt tatggtaatt catgtgcagg aatttatggg agttctgcct ttcctgacaa 41040 ttgtagataa ccactcctgc atatcatttc ttccatataa tccagtgttc actgatggga 41100 gcattaactc agtttcatga gtcacttttg ctttatatct gtagagagca tatagggaac 41160 tttttcatat catcaatccc agacacctca ttttttatct gaaatgtata ttttccagcc 41220 aagaaccttt tattgcttag acttcttcac atctgttatc aacttcagta gctgcttttt 41280 tttttttttt caggataatt tttacgaaga ggggactttt aaagtcattt ttctcatttc 41340 tgagtacatg gcaacctgta aatgacatca aagccaaaat cagatgtaga agccgggcag 41400 ctagccagac tcattctacc ccaagtgact gacttccttc ccacacacca tcatcactac 41460 acacgatttt taggatcatg ccttagcaaa attacgtgat agacctttta gaccactcac 41520 atgtagtgga aaccaaactt tagaggccaa accaaagatc ctgctttatt tcactttgct 41580 gtagttttgt gatattatca ggtgtgctct gaagtgtgta gagccgtttg tccctgcatt 41640 aaatggtaca gattgtcatc tgttttcaat tcttatatct gctttttatg gctttttctg 41700 tctgtaccct tgtttggtga tgacttctct ttcctttgca tgcctttagc agccctcttt 41760 ctatttagat tttgtaatta ttagtctact gtgaatttgg agaccagacc acaaaaattg 41820 ttagaattgt attttcatga aaagtctgta agcctggagt cctcttgtga gtaaagctca 41880 ttcatatagg gattaggctc gctccagaca tggcaagtga agttggtgaa taagtgacta 41940 tcagagaagt atgggatatt tgggatattc ttgttaccta tggcattata ttatagttgt 42000 attattttag ttattcctaa agtagttacc atttcttttt ctttttccat aggagcttcc 42060 atgtaccagt ctcaaaagtg aaggtatgac tggggtgggg catgagggaa tttctggggt 42120 gaaagtgtcg tcactctgaa tcttgttagg gagttgggtt atgtatttgt caaaattcag 42180 tgaaggcata cttaagattt gtggtataac tttcacctca aaaggaaaaa aatataaatt 42240 gaattctaat tgatgaaatg ctgaaaaata taagctaagt gcactcatgt ctacagcttg 42300 ctttgaaatg cattaaaaaa gaaaattagt gggtaggtag aagaatggat atgtatatga 42360 aaaaatatgt taatggtaga atctagatat gggcatatgg atgttcactg taaaatactt 42420 tgaacttttt ttctttttcc ttttgagacg gagtctcgct ctgtttcgca ggccagagtg 42480 cagtggcgcg atctcggctc actgcaacct ccacctccca ggttcaagtg attcccctgc 42540 ctcagcctcc caagtagctg ggattacagg tgtccaccac tacacctggc tgatttttgt 42600 atttttggta gagacggggt ttcactgtgt tggccaggct ggtctctaac tcctgacctc 42660 aagtgatcca cccgcctcgg cctcccaaag tgttgggatt acaggcatga gtcaccacac 42720 cggcctcgat tcctgttttt ataaccgaca catactgttg ttgaagccat tttaaaacct 42780 ttttggaaaa gtgatggaag ataaggatat gcaaaataaa aggtcttact gtagtcagaa 42840 agaaagctgt attggttagg aagcttctgg atgcaggtaa caaaacccaa gaaaaagtga 42900 cttttacgat aagggtgttt actgccacac ctgataggat atctggaggc agaacaagtc 42960 cgagattggt taattttagc tcagtgattt tgaagacttc aatttggctt ctctgtggtt 43020 ttcttgattt tctcctcgtg ggtatgttag ctgctgcagc ttcacacatt gggtctctgc 43080 ccaacaacat ctaaacaaaa tccatgaaga gagtgttgtt gttctcttcc tatgtaagag 43140 caaggaaaac agtcctgaaa ggttctcagt acactgtgtc caattcttct gcctaaacct 43200 gtaactagca aagtgaatgg aattaccatg gtgggtaaag gtacacaaca tccacccctg 43260 gaactgagga gcacactggc tcccagtttc tgaacaaaat taaggctgtc tttgcctgaa 43320 agagaagagt ggccgtttgg gaggtaccca gcaatatctg cctcaaaacc gtatttaaac 43380 ttttggttca gtgtgccatc tgtatcaagg aaaaaaagtt tggttattaa aaacataatt 43440 tgggtccaag cctggtggct catgcctgta atcccagcac tttgggaggc caaggtgggc 43500 ggatcacctg aggtcaggag ttcaaggcca gcctagccaa catggtgaaa ccccatctct 43560 actaaaaata caaaaattag ccaagtacag tggcgcaccc ctgtaatccc agctactcag 43620 gaggctgagg caggagaatc acttgaactc aggagacaga ggttgcagtg aactgagatc 43680 acaccattgc tctccagcct gggcgacaga gtgagactcc gtatcaaaaa agaaacaaga 43740 aaaacataat ttgggccaga tgcagtggct cacatctgta atccttatgc tttgggaggc 43800 cagggctggg ggatcactta aagccaggag ttcaagacca gcctgggcaa catagtgagg 43860 cactgtcttt gcaaaagaat ttttaaagtt agccaggcat agtggcatgt gcccatagtc 43920 ctagatactc gggaggctga ggcagaggat tgcttgagtc caggagtttg aggttgcagt 43980 gagctgtgat tgtgccattg cactctatcc tggtgacaga gcgagaccct atctctaaaa 44040 caataaaaca aaataataaa aacataattt gaaaaaatac aaaattaata cacttatgaa 44100 gtttaaaaaa aaatatgaag tgaaagatga aaatttgccc tctaatagta gtttgttagt 44160 tagggtttca gatcttttt cctattcata cagatgtata aacatgccag catagttttg 44220 tttttcccta gatttaggct tatactaaag atactgtctg cagcttattt tccctcacta 44280 atatctcaaa gacgtatttc catgtcagta tgcataactc ttgttcttgc agagtattcc 44340 atatgtggat ctgccttgtc atagttcacc atgacccttt tggtggtcat ttaggttgtc 44400 tccagttttt ccccattatt aataagcagg aaccgtcctg tcccactttt tataacttta 44460 tgatgcaaaa tttcaaccat acgtgaaagt ggagaaactg gtataatgaa tccccacgta 44520 cctatgacct gtcatcatca gttgtcaaca ttttacctct cgttttcatc catccctcct 44580 tccctacatt ttatttatgc tgaagtactt tgaaaacaaa tctcaaatat catttcaccc 44640 ataaatactt cagtggttag cccacttttt ttttagttga aattcacata acataattaa 44700 ccattttaaa tgtacagttc agtggcactt agttctttca caatctagtt ccaaaatatt 44760 ttctctgtct tacatttttt cttcttctct attttatctt tctttttctt tttttttttt 44820 ttttttttga gacggagttt tgctcttgtt gcccaggctg gagtgcagtg gcgcaatctc 44880 agcttactgc aacctccacc tcctgggctc aagcaattct cctgtcttag cctcctgagt 44940 agctggaatt acaggcaccc accaccatgc ctggctaatt tttttgtatt tttagtagag 45000 acggggtttc accatgttgc ccaggctggt ctcgaactcc tggcctcatg taatccgcct 45060 gcctcagcct cccaaagtgc tgggattaca gtcatgagcc accgtgccca accttttttt 45120 aaattttatt ttaaaaatat gttgagacag ggtctcactt tgttgaccag gctggtcttg 45180 aatgcttggt ctcaagtgat cctcccatgt tggcctccca aagtgctggg gaaaaacatt 45240 ttcgtcaccc tgaaaggaag ccctgtatta attaagcatt tactccccac tcagtcctcc 45300 cccaggtaac cactgatctg ctttctatct gtatggattt actattttcg aatacttcat 45360 ctaaatggag tcacaatata tattcttttg tgccctgctt ctttcactta gcatgttttc 45420 taagtttatc atactgtagt atatatcagc acttcattcc tttttatgat tgaataataa 45480 ttcattttat gaatatacat tttatccatt cactggttga tggatattag aattgtttct 45540 actttttggc tattgtgaat agtgctgtta tgaacatttg tatacaagtt tttgttccaa 45600 tacctatttt catttctttg gggtatatac ctaggagtag aattgctgga tccaatggta 45660 atcttgtgtt taattttttt gaaaaactgc cattctgttt tccacagtgg ctacaccatt 45720 ttatattcct accatcagtg tatgagggtt tcattttctc cacatccttg ccaatacttg 45780 ttattttctg ggtttttttg gtttttgttt ttttgagatg gagtcccact ctgttgccca 45840 ggctggagtg ctgtggcgct atctcagctc actgcaaact ccacctccca ggttcgagtg 45900 attcacctgc ctcagcctcc cgagtagctg ggattacagg tgcccgccac cgcgcccggc 45960 taatttttgt atttttagta gagatggggt tttaccatgt tggtcaggct ggtctcgaac 46020 tcctgacctc gtgatccgcc tgcctcagcc tctcaaagtg ctgggattac aggcgtgagc 46080 caccgcaccg accatttttg gggcttttta aatcatagcc atcctagtga agtgatatat 46140 cactaccaca tgatgtgatg tctctgatat ctcaatgtta ggttttaaaa tctatttatt 46200 gtttaagaca gggtctctgt caccttgact ggagtgcagt ggcagaccac agctcactgc 46260 agccttgatc tcctgggctc cagtggtcct cccacacaag cctcccaaat agctgggacc 46320 acaggcatgt gccaccatgc cctttgctta attaactttt aattaagttt taaaaactta 46380 atttttaaaa gtttttccag ggctgggcgc agtagctcac gcctgtaatc ccagcacttt 46440 gggaggccaa ggcaggcgga tcacttgagg tcaggagttc aagactagcc tggccaacaa 46500 ggtgaaaccc cgtctctact aaaaatacaa aaaattagcc ggcatggtgg caggcacctg 46560 taatcccagc tactcgggag gctgaggcaa gagaatcgct tgaacccagg aggcggaggt 46620 tgcagtgagc aaatttttgt agagacaggg tctcattatg ttggccaggc tggtctcaaa 46680 ttcctgggct cgagcaatcc tcctgcccta gccacactta aattttcgat agaaacacac 46740 tcagccagcc ttccatatcc atgggttcca tatccatgga ttcaaccaac cacagattga 46800 aaatattcaa agaaacaaat tacatctgta ctgaacatgt acaaaatttt ttctcatcat 46860 tttctaatag catttacatt atatttggtg ttataagtaa tctagagaag atttgaagta 46920 taagggaggg tgtgcatagg ttatatgcaa atattatacc attttgtata agagacttca 46980 atatctgtgg attttgatat ccaagggagg tcctggaatc agtcccccat ggactccaaa 47040 agtttgtaca cccatctact aagatgagca tggcttctcc tccacacctt aaacactacc 47100 gttgctattt tttttatttt ttgagtttta aaggtgaaaa ttgatatttt aatatgcatt 47160 ttcagctggg cagagtggct tacacctgta atcccaacat ttggaaggcc aagatgggag 47220 gctcatttga gtgcaggaat tcgaggttac agtgagctat gttaatgcta ctgcactcca 47280 gcctgggtga cagaaagaga ccctgtctct ttaaaacaaa acaaaacaaa acaaaacaaa 47340 aactgatggc cgggcatggt ggttcacacc tgtaatccca gcactttggg aggtcaaagc 47400 aggtgaatca cctgaggtca ggtgttcaag accagcctgg ccaacatggc gaaaccctgt 47460 ctctactaaa aatacaaaaa ttagccgggc gtggtggcgc acacctgtaa tcccagctac 47520 tcgggaggct gaggcaggag aatcgcttga acctgggagg tggaggttgc agtgagccaa 47580 gattgcgcca ttgcactcca gcctgggcag cagagtgaga ccctgtctca gaaaaaaaaa 47640 aagaaagcaa aaaggccagg cgtgttggct cacacctgta atctcagcac tttgggaggc 47700 catgggaggt ggatcacctg agatcaggag ttaaagacca gtttggacaa catggtgaaa 47760 ccctgcctct actaaaaata caaaaattag ctgggtgtgg tggcaggcac ctgtaagccc 47820 agctacttgg gaggctgagg catgagaatc acttgaacct gagaggtgga tgttgcagtg 47880 agccgagatc acaccactgc actccagcct gggcgacaga gaaagactcc atctcaaaaa 47940 acaaaacaaa acaaaaaaaa ctgcatttac attattacaa ttgtattatt actattgtat 48000 tctttgagcc tatagttact caaatatttg ttgtatgaat gttcatatcc tttgtctggt 48060 tttttttttc tttttaattt gccacagcag atgctacatt tatgtgtgac aagcggtgta 48120 acaagaaacg gttgtgtgga cggcataaat gtaatgagat atgctgtgtg gtaagtggac 48180 ttattaggca taatcagatt ccattcatcc aggtgctggg cttctggaag cctgagccat 48240 gtggagaatg cagcccggat ttaaaagtca ctgggacagg cccctaagca gcagtcagag 48300 agctctcata gtccccataa cctctctgca aagagacaga ctgggccttt ttagcctcac 48360 ccaggttaag aagtcagcca gtggcatctg gtagcaacac agaggaagag aaagagataa 48420 gagaaggtct cacaccccag agcccaagac ccctagatga gcacacagtc ttcggcagaa 48480 gattgtgtgt tgtcccaaca caaactggcc tggacttagc aacttgggct tcaggtttca 48540 tgtactgctt aggtcaggtc agtaggaaaa attattagga cattttttaa aaagattttt 48600 taaaataaaa tatttattta aaatatttgc ttttatggaa ctaattaaat cactagctgg 48660 ctatttgtac tctgttatct catatactct tttatatata aaaaatataa cgtatgctat 48720 atatgacata acatgtatat ataaatgtgt atgtatgtat tagtccattt tcacactgtc 48780 agtaaagaca tacccaagac tagggaagtt acaaaagaaa gaggattaat ggatctacag 48840 ttccaagtcg gtggggaggc ctcacaatca tggtggaagg caaggaagag caagtcactt 48900 ctcacatgga tagcagcagg cagagaaaga gagtttgtcc aggaaatggc cttagaaagc 48960 catcagatct catgagactt attcactatc acgagaacag cacaagaaag acctgccccc 49020 acgattcaat tacctcccac caggtccctc ccacaacatg tgggaagtca agatgagatt 49080 tgggtgggga cacagccaaa ctatatcatt ctgcccctgg cccctcccaa atttcatgtc 49140 ctcacatttc aaaaccaatc atgcctttcc aactgtcccc caaagtctta actcatttca 49200 gcattaacgc aaaagtctac agtccaaagt ctcatccaag acaaggcaag tcgctttgcc 49260 tatgagcctg taaattcaaa agcaaattag ttacttccta ggtccaatgg gagtacaggc 49320 attgggtata tacagccatt ccaaatggga gaaattggcc aaaacaaagg ggctacaggc 49380 cccatgcaag tccgaaattc agtggggcag tcaaatctta aagctacaaa atgctctcct 49440 ttgactccat gtctcacatc taggtaacac tgatgcagga agtgggttcc catggtcttg 49500 gacagctccg cccatgtggc tttgcagtgt gcagcccccc tcccagctgc tttcacaggc 49560 tgtcattgag tgtctgtggc ttttccaggc acacagtgca agctgtcagt ggatctccca 49620 ttctggggtc tggaggacag tggccttctt ctcacagctc cactaggtgg tgccctagta 49680 gggactccgt gtgggggctg caaccccaca tttcccttct gtactgccct agcagaggtt 49740 ctccatgaga gccccacccc tgcagcaaac ttctgcctgg acatccaggc atttccatac 49800 atcttttgaa acctaggcag acgtttccaa cctcagttat tgatttctgt gcacccgcag 49860 gctcagcacc acatggaagc tgccaaggtt tggtgcctgc actctgaagc cttggcccaa 49920 gctctgtgtt ggcccctttc agctacagct ggagtggctg ggatgcaggg caccaagtcc 49980 cgaggctgca tgcagcaggg ggaccctggg gccagcccac aaaaccattt tttcctcctg 50040 agcctccagg cctgtcatgg aaggggctgc catgaagacc tctgatgtgc tctggagaca 50100 ttttccccat tgtcttgggg ttttacattt ggctccttgt cacttatgca aatttctgca 50160 gccagcttga ttttcttctc agaaaatagg attttctttt ctctcacatt gtcaggctgc 50220 aaattttcca aacttttatg ctctgtttcc cttttaaaac tgagtgcctt taacagcacc 50280 caagtcacct cttgaatgct tcgctactta gaaatttctt ccaccagata cccttaatca 50340 tctttctcaa gttcaaagtt ccacagatct ctagggcagg ggcaaaatgt tgccactctc 50400 tttgataaac aacaagagtc acctttgctc cagttcccaa caaattcctc atctctatct 50460 gagaccacct cagcctggac attattgtcc atatcattat cagcattttg ggcaaagcca 50520 ttcagcaagt ctctaggaag ttccaaactt tcccacattc tcctgtcctc ttctgagccc 50580 tccagactgt tccaacatct gcctgttacc cagttccaaa gtcacttcca cattttcggg 50640 tatcttttca gcagtgcccc actctgctgg taccagttta ctgtattagt ctgttttcac 50700 actgctgata gagacatatc caagactggg caagttacaa aagaaagagt tttaatggac 50760 ttacagttcc aagtggctgg ggaggcctca caatcatggt gggaggcaag gaggagcaag 50820 tcacatttta acatggatgg cagcaggcag agggagagag cttgtgcagg gaaactcggc 50880 cttataaagc cattagatct catgagactt attcactatc atgagaacag cacgggaaag 50940 acctccccca ccatgattta gttaactcct accaggtcct tcctacaaca tgtgggaatt 51000 caagataaga tttgggtgga gacacagcca aactgtatca gtgtatatat atatacataa 51060 acaaggtaac acagtaccaa tagcaaggta gtacgtgtgt atgtatgtat atagcataca 51120 taattatttt ttaaatttat atatttaagt gaagagtgat gaattgtgca agtattataa 51180 actagtgtta gaaggcttaa agacttcctc ttattataaa aaaatttgca gccaggcatt 51240 gtggctcaca cctgtaattc cactttttga ggccgaggtg ggtggatctt gcttgatcca 51300 aggaattcaa ggccagcctt ggcaacatgg taaaacccca tctctgccaa aaatacaaaa 51360 attagccagt ttcataaccc ggtctctaaa taaatagatt taaatgtaaa aataaggctg 51420 gatgcggtgg ctcatgcctg taatcccagc actttgggag gcaaaggcag gcagatcact 51480 tgaggtcagg agttccagac cagcctggcc aacatggtga accctgtctc tactagaaat 51540 acaaaactta cctggacatt gttgtgggca cctgtaatcc cagctactca ggaggctgag 51600 gtaggagaat tgcttgaacc caggaggtgg aggttgcagt gagccaagat cgcaccacca 51660 cactccagcc tgggcaacag agtgagaccc tgtctctgtc tctctctctc acacacacac 51720 acacacacac acgatgtaaa aataaaatat atttttttaa aatttcaaac tggctcatgc 51780 ctgtaacccc ccagcacttt gggaggccaa ggtgggcgga tcatgaggtc aggagattga 51840 gaccatcctg gctaacacgg tgaaacctcg tctctactaa aaatacaaaa aaattagcca 51900 ggcatggtgg tggatgcctg tagtcccagc tactcaggag gctgaggcag gagaacggtg 51960 tgaacctggg aggcggaact tgcagtgagc caagattgct ccactgcact ccagcctggg 52020 cgacagagcg agactccatc tcaaaaaata tatatgtatg tatcaaatat atgcagaagc 52080 agagagacta ctagtataga gagtcccata tacacaacat ccagtttcag tattgatcac 52140 ctcatgacga attttgtttt atcttcatcc ccacctactc tcccttctgt ggctattttg 52200 aaggtatttc cagatatcat tttatccata aatgtgtaga gctttgtttt tattaatctt 52260 gttgttattt atggaaaata taaaatgaag accattttat gaacaaatat aaatctcttt 52320 tcccaggata aggagcacaa gtgtcctttg atttgtggga ggaaactccg ttgtggcctt 52380 cataggtgtg aagaaccttg tcatcgtgga aactgccaga catgctggca agccagtgag 52440 tgtctttact gtatagttta ttagaagagt tttttagaaa ttcagacata atatactttg 52500 agagattgat ttacttagaa ttgtatggat tcttgaacta agccttcgta tttccaatat 52560 gttgagactt tttttaaagt tacttctaat gctgtgttgt agtccctgaa tgtgcaaaac 52620 ttgggatatg aacagtgatc ctgagatggg gcctagttcg ttaatagatt ccttctctgc 52680 tacatgcctc atatgtgtta ttgacctagg ttctgtcctc aacaatcttc tttctctata 52740 ttattagcag cagtttgcaa actttttggt tgtagggctc atttacatta ccaaaaacta 52800 tcctaaagag tcatttttat gtgaattgta tttatcttta tctaccatat tagaaattaa 52860 aactgagaaa tttaaaaaat aattgattag tatatttaag aataataata tacctcattg 52920 catgtaatca caaaaatttt tttaaataac ttttcaaaaa agtagaatag cggatacttg 52980 gtttaataga aggtagctgg attcttgtat ctacttctgc atccagtctg tcgtgatata 53040 gttgaggcat atgaaaaaaa tccagcctta cacagatagc cttacacagg agaagggaag 53100 agtattttaa agatgttttc aaataattat tgatattctt tgaaattaca ccaaaattca 53160 acctcatttt agaaaggact tgactcggtg tttttattct ctgtcacaaa aatttattgg 53220 actttcttgt actttgaatg gatcttttta cccgtgcatg attttataat agcccacatt 53280 gatcatttgg aaaataattc ttcactgagt tatgtgatct ttgaaatgtt gataaatttc 53340 ctattataat attaaaaact cagtcattaa tataatctcc agtctagaaa tatgttggga 53400 aaagtattgg gaagttgtca gggtcatagt aataaatgaa acttcatatc tgttcatgcc 53460 tttttcacca gcttagcttt aataaaataa aagtttaata aaataaaaac ctcttttgaa 53520 aaaagtagca cccccataaa ttctcctcta aaaatacaag caaaaatgtg tgtgtttgtg 53580 tgtgttagta tgtacttgca catcccaaac tcagaaagga ttcttttgtt ttacctgctg 53640 ctccaccagg ttttgatgaa ttaacctgcc attgtggtgc atcagtgatt taccctccag 53700 ttccctgtgg tactaggccc cctgaatgta cccaaacctg cgctagagtc catgagtgtg 53760 accatccagg tgagtaccaa cttgtcttca cacttcagcc tgctcacacc atgtcattta 53820 ggatcctgct gtgttactgt cttcactgct ctagagtcat gctgtgatgg ctgccccttg 53880 ctcccggctc aggggctggt tctctgaagg tgggtctgtg atctgggcca cagcttggaa 53940 tcacagtgcc ctccattggt ttactctagg gatgtcaaac acacctaaaa acgttggaag 54000 actccccagc tgatcttatc tctcaagaac ttttctttct ttgtggtctc cactttggtg 54060 catgttttga aatttagcat ctttttacaa aattaaaagt gacttttttg ttgttataga 54120 agcaatatat atttatcgta ttaaaattaa gaaaatagag gccagggcca ggcgcagtgg 54180 ctcatgcctg taatcccagc actttgggag agcaaggagg ggcagatcat gaggtcaaaa 54240 gattgagacc atcctgccca acatggtgaa accccgtctc tactaaaaat acaaaaatta 54300 gctgggcatg gtggtgcacg cctgtagtcc cagctactcg ggaggctgag gcaggagaat 54360 cacttgaacc cgggaggcag aggttgcagt aagctgagat cgcaccactg cactccagcc 54420 tggcgacaga ccaagactct gtctcaaaaa agaaaataga ggccaggcac agtggctcat 54480 gcctgtaatc ctagcacttt gggaggctga gatgggcggg tcacttgagg tcaggagttc 54540 gagaccactc tggccaacat ggtgaaacca tgtctctact aaaaatacaa aagttagcca 54600 ggcattgtgg cgggcacctg taatctcagc tactcaggag gctgaggcag gagaattgtt 54660 tgaatccggg aggcagaggt tgcagtgagc caagattgtg ccactgcact tcagcctggg 54720 caacagagtg agactccatc tcaaaaaaag aaaagaaaaa gaaaaagaaa aaaaaggccg 54780 ggtgcagtga ctcacacctg taatcccagc actttgggaa gctgaggcag gtggatcacc 54840 tgaggtcagg aggttgagac cagtctggcc aacatggtaa aaccctgtct ctactaaaaa 54900 tacaaaactt agcctggcgt ggtggtggac gcctgtaatc ccagctactt gggaggctga 54960 ggcaagagaa tcacttgaac ccgggaggca gaggttgcag tgagccgaga ctgtgccatt 55020 gcactccagc ctgggtgaca gagtgagact ctgtctcaaa aaaaaaagag agataaagtc 55080 aaaagtttga aattaaataa tctattatat gtcacctcca aagaattata ccttatatgt 55140 atttctcagg gtcttttgcc atgtgcaaat atatatgttt gcattttttc cccaatgtaa 55200 aagtgggatc ctactgaact taatgctttt aactagcttt ttttcccact cttattatta 55260 tatttttgat ctgtcccctt gtaagtattt ttttacaata taaactttta atttcaattg 55320 agtgtcctgt tacatggatg ggccataact tattcagtca gtcttgtgtt gctggacatt 55380 ttagtgtttt cagttttcct ctgataaact gctcagatac caggccaaca accatttgac 55440 atcactcaat ttccgtatct gtcaagtcta gttgctggct tttctctagc accataccgg 55500 gcccatcatt tcattttacc aaataccaga ggcaaacctg aagtaattca ggggaccaga 55560 tttgataccc acagttagtc tggcagtatg attcagactt aaaactagaa aaccaactct 55620 tgatttttta agttaatgga gtaaaacaga acttaacaaa ttcttcatta agtggaatgt 55680 tccctccttc cttggggatc ttaggatagc cctcccagcg tagtttgcaa gcccctcctg 55740 tggcattcat accagcatga agtacatgcc caggccagtc cccaccgatt gcctccttta 55800 cacttaacct gcagatgaat tccaaatgct gctgggcaag gtccatagta agaacttcac 55860 catttgtgaa ttgtgttgtt ttagcctatt ttgttcccca ttcatttcca ttttcttaag 55920 tgtttattgt atttctaaca tggtgtcaga catagaggga acttcagagg aagagaaaaa 55980 taccctgtga ccttgaggtt cttaacattc tccgagagga ggaggacagt agttcaatac 56040 acatgataca accacccttc acatgaagat gggctaaata atgtggcagc aacccatctg 56100 cataggaact gagacaccag gaagtgtgag ggagcccaga cgagagtgtg gtagccaagg 56160 aaaggttcat tgtaggaggt gagttttgat gaacctcccc ttataggcag taatgaaatt 56220 ggaaggagtc agcagaagca ttttagatgg aattaatagc aaagatatct gagcagaagt 56280 gagcagttgc ggttaaagta gggccaaaag aatgacgtag tagaattgga tggaccatat 56340 ggctgtgctg caagagatga gatgggaaag ctgcctcatc tgcttctgtt ttggcatgtc 56400 tcttcctcct tggttgggtt tctttttctc actcatgctt gtccttcaac actttaacat 56460 aaagcaaggt actttttact ttcctgtgac cgtttttcaa aagtactccc agtttctgaa 56520 tttaaaagtt tcatgccgta tgccacttat atgatgtata atggtttaca tggctttatt 56580 tagaaagtat tcctaaagtt acctttctct ttctgcagta tatcattctt gtcatagtga 56640 ggagaagtgt cccccttgca ctttcctaac tcagaagtgg tgcatgggca agcatgaggt 56700 aagttttctc tctcaagtgc tcattgttcc aggcagaggt tcatgatttt attacttaag 56760 aattgttagt ctcatcgtct gtcaaagtaa atacactcag aagtagaagc tttccattgc 56820 ttaaaaattt tttcttaaga tataatttgc atacaacaga atttaccctt tttattgtac 56880 agatccatga gttttagcaa aagcatacat accatcaaac cactaccaca atcaagatat 56940 agaacagttc agtcaccccc aaatatcctt catgcccctg tatactcaat gtcccctact 57000 tccaacttct gaaaaccaat gttgtctgtt ccaatcattt tactttaaaa taccaaattt 57060 ttttttaata acctccttat tcctgcaaga atggcaataa tcaaaaaata atagatgttg 57120 gcgtggatgt ggtgaaaagg gaacactttt acactgctgg tgggaatgta aactagtaca 57180 accactgtgg aaaactgtgg agattcctta aagaactaaa agtagattta ccgtttgatc 57240 cagcagtccc actactgggc gtctacccag aagaaaagaa gtcattatat gaaaaagata 57300 cctgcacacg catgtttata gcagcacaat tcacaattgc aaaaatgtgg aaccagccca 57360 actgcccatc agtcaacaag tggataaaga aattgtggtg tatctatacg taccatggaa 57420 tactactcag ccaggaacga aataatggca ttcacagcaa cctggatgga tttggagacc 57480 attattctaa gtgaagtaac tcaggaatgg aaaaccaaac atcgtatgtt ctcaattata 57540 agagggagct aagctatgag gacgcaaagg catgagaatg atacaatgga ctttggggac 57600 tctggggaaa ggacgggagg cgggtgaggg ataaaaaact acacactggg tccgagcacg 57660 gtggctcatg cctataatcc cagcactttg ggaggccaag gcaggtagat tatgaggtcg 57720 ggagttcgaa accagcctgg ccaacaaggt gagacccccc ccatctctac taaaataatt 57780 agccgggcct ggtggcgcat ccctgtaatc ccagctactt tacttgggag gctgaggcag 57840 gagaattgct tgaactcggg aggcggatgt tgcagtgagc caatatcgca ccactgcact 57900 ccagcctggg caacagagca agactccgtc tcgaaaaaaa aaataaattt taaaaactac 57960 acattgggta caatgtacac tcctcgggtg atgggtgcac caaaatctca aaaatcacta 58020 ctaaggaact tatccatgta accaaacacc acctgttccc caagaactat tgaaataaga 58080 aaaaagaaaa aaaaaaaatc actgattgca ggtcacccta acagatataa taatgaaaaa 58140 gtctgagatg ttgcgagaat taccaaaatg tgatgcagac acgaaatgag catgtgctgt 58200 tggaaaagtg gaacagatag acttgctgga gacagggttg ccacaaagtt tcaatttgta 58260 aaaaatgtga tatctatgaa atgcaataaa gtgaagtcca ataagaaatg catatttatt 58320 gtacagttga gaagatttaa ttctgctact gagaaagaaa tactctacca ttctggcata 58380 taaaccacca gacgtggtac acatttttcc ttgctgtggt taagatgata ctgtttgcta 58440 cattccaact gtcctatacc aaaaaaaaaa agatattgtt ttgtaacctg ctttttttgc 58500 atcatgacgt attggccaac atttcatgaa actcttgaca tctataacaa catgctatat 58560 tcttttatct tcatggacgt atattcctcc actgatttcc tgtgggtggt tctcaagtcc 58620 ttggtattta ttcttttcta tatgtctcac cttttccagt ttatccattc taaaacatca 58680 atgtgttatc ttcgtactga tggcttatta agacttcatt caacagatat cgagtatgag 58740 tacctactat gtgctaggta acaggctagg tttgggtgct ttggaagtga acgagactaa 58800 cccttgccct tatgaagcct tcactttggg ttaggaggca ggcatgaaaa cagacaatag 58860 cagtaatgcg ggaaatacag agggctaagg aggatggaag ggctctccag gtgttctgga 58920 aaaattaaca tcctaaagga tagtgagaag aaagtaatgt ggagaggatg acagggcaga 58980 gcatgggcaa aaattatccc ctttgccctg gtagtagaat gggtaagtgt ttaatgattg 59040 agaactacat tctactaaaa tatggattcc agtgcgatgg gaaatatgta tgtcctagat 59100 gttggaagtc tcttcagggg gagaagttgt gtgtcactgc agtagaagta tatccctcat 59160 ttcgagggtt caagactagc ctttggtcct acgagagtaa aatttcagca gctttttcct 59220 atggtgagga agcgtggaaa caggccattt ttcaagaaaa aaaaaaagaa ttgggggttc 59280 tatcccctgt tgtctcttaa ttttttaaag gacaggactg cgtgaagctg tggagaccat 59340 gagttgggag acatgaggag agttaagtat ttaattacag aaaacagcct cctagcctgg 59400 gcaacctagt gggatcctgt ctctacaaat aatacaaaaa ttagctgggt ttagtggcat 59460 gcctgtactc ccagctactt ggaaggctaa ggcaggagga tcacttgaac tcaggagttc 59520 aaggctgcag tgagacacga ttgtgcaact gccctccagc ctaggcaaca gagcaagatt 59580 ctgcctcaaa aaaaacaaaa caggccaggc gtggtttctc atgcctgtaa tcccaacact 59640 ttgataggct gaggcaagtg gatcacctga ggtcagcagt tggagaccag cctggccaac 59700 atagtgaaac cctgtctcta ctaaaaatac aaaaattagc tgggcatggt ggcacacacc 59760 tgtagtccca gctacttggg aggctgaggt aggagaatcg cttgaaccca ggaggtggag 59820 gttgcagtga gctgagatgg cgccaccgca ctccagcctg ggcgacagag cgagactcca 59880 tctcaaaaaa aaaaaaaaaa aaggcaaaat aaaacgaaaa aatggccctg ccatcagtct 59940 cagaaccctg ggctaaggtt gccctccacc ccgcatctat aagaattagt ctattgactc 60000 tgctttagca gttcagaaag gagggaaaag atcatctgcc cctgccagtt agtcatagca 60060 ttttcaagca ctccctagtt acctagttac atttgtttgt tgtcactttg gaaagtttat 60120 gttccagatc acatttttgg cttctgctgc aaatcctttt gagtcataac agggattatt 60180 aaataaagtt aaggtttgtt ttcttcatct caaactaacc tttcagactt tgaaaatcaa 60240 actgatttct tttcagataa atagagcttt tttaaaaata cagcttttaa gctggatgca 60300 gtggcatgta cctgtagtcc cagctaatca gaagactaag gcaagaggct cacttgagcc 60360 cagaagttca agtccagctt aggcaacata gtgagaccct gtctctaaaa attaaagtaa 60420 taataatcct tattttaaaa actgagctga gtgtggtggt tcatgcctgt aatcccagca 60480 ctttgggagg ccaaggcaag tggatcactt gaagtcagga gttcaagacc agccatctgg 60540 ccaacatggc gaaaccccaa ctaaaaatac aaaaattagc tggtcgtggt ggtacacacc 60600 tgtaacccca gctactcggg aggctgaggc tggagaatca cttgaaccct ggaggtggag 60660 gttgcagtga gccccgagat tggcgccact gtactccagc ctgggcaaca gagtgagact 60720 tcgtctcaaa ataaataaat aaatacaaac tgttattgcc agatgcagtg gttcacacct 60780 gtaatttcgg cactttggga ggcctaggcg ggtggatcgc ttccactcaa gagttcgaga 60840 ccagcctggg caacatggtg aaaccccatc tcttaaaaaa aaattcaaaa attagctggg 60900 cgtggtggca tgcacctgca atcccagcta cttgggagac tgagatggga ggatcacctg 60960 agccccagga aagtcataat cacactgttg cactccagcc tgggcaacag tgaaacccta 61020 tatccgaaaa caaaacaaaa ccaaaccctg ccataagctt agaaatgttt aaagagaaag 61080 ttaaaactca ccccaaatcc cacatggaga tgagaatctg gctacttctg tctctcctag 61140 tttcggagca acatcccctg tcacctggtt gatatctctt gcggattacc ctgcagtgcc 61200 acgctaccat gtgggatgca caaatgtcag agactctgtc acaaagggga gtgtcttgtg 61260 gatgagccct gcaagcagcc ctgcaccacc cccagagctg actgtggtca cccgtgtatg 61320 gcaccctgcc ataccagctc accctgccct gtgactgctt gtaaagctaa ggtgggtatt 61380 tctggccaca gatgcagcat tgactgtagt tctgaggcta ctagtgaatc cagaactgtc 61440 tacagtggcc cctgggcatg catcttaatt aagggtcatt ggttgcaaat aaccacaagt 61500 tagagaaagg tagagtaagt catacaagtt tctgcctgcc caacttggta tggccttttg 61560 attcagcagg tacccactgc caccggacca cagtctgtgt ctcccttggg tgagattctc 61620 aatagtcact gattgtttac tccatttatt taataaatgt ataaatgtct tttgtgcaag 61680 acatagtatg aggtgctagg gataaattag tgaacaagag acacatggtt cctcacaatc 61740 tgataggaca gatacttgat tattacttag ataactaatt atggctgtgg tgtcatgagg 61800 gagtagttta agccttgtgg agggggctat gagagcaggt aaatgggagg ttaagcctgg 61860 tctaaaggat caagtgtggc caccttgagg atgtggagtt ggcttggagt tggctaggcc 61920 acagttgggc ctagggcaag tgtctgtctc tgatggtaca aacttcatcc taggcctgcc 61980 ccttcagcca ggattaagaa cagaggtggt ttccaaaagg gggcattgtg gacggggcaa 62040 ttctttccta gcatgtcaac aactgtgtca gtttgcatgt gtggttagct tcttccagct 62100 tggagagtgg ccacccactg actctctagt ctctgctatg gtttaagaga ggatttaaga 62160 gtgaaaagtg ctttattcac atagttccag tgtgctaaaa gtcgttccat gttcatctga 62220 cttcaggtag agctacagtg tgaatgtgga cgaagaaaag agatggtgat ttgctctgaa 62280 gcatctagta cttatcaaag gttagtgtta cttaaatgtt aacaactgat ggcctaggtg 62340 aaacagatac atgtgttttg ttttgttttt aaagctagtc aagtgaagca gtgggagtgg 62400 agaagaaacg aagtctgtaa ctgttgtaat cagttgtaaa caccactgca cagatacat 62460 tgtttatcat agtatccttg caacttttct aaaagctgga aaggtttttt tgtttgtttt 62520 gttttgtttt attgagaggg gtctcgccct gtttcccagg ctggtctccc accttggcct 62580 cccaaagtgc tgggatcaca ggcgtgagcc actacacctg gctggaaagt ttcttacata 62640 catttttaaa aatctggttt ctctagttca aagaaacagt gctgctttat ataccttttc 62700 ttccctaact ttagtgtgta aaataacttt gaaatttctc cgcttcctcc ttcctctttc 62760 tttttttctc tcttttggtc gtattacatt gtgagataag aagggaagat gtcctgaagt 62820 taggtgaatt gctttcacat cctaccattt gtcattgaca cagccagggc taagctctgt 62880 gctcctctgg tgtgagggtt tctaattgtt ctgtgaggta tctctgcagc ctcctgaagt 62940 cttgggacct gtgtagccgt acagggatct gagtgctgag atggaggtgt tagagaatga 63000 atggttatat atatatatat ctggttagat ggggagaggt gccatggata taggacttta 63060 agaggatgca cacatcagat tggctgtgtt tgaataccta ctaagtgcca agtgttgctc 63120 taggggatac tgagggattg agtcacctca tcgcttccca tgtagctgct ttgaatataa 63180 ttgaagagat atgacataaa tatgtaggta gttcatagta gccgggcttc caaggccaca 63240 ccagttttca tctgatagat ttgctttttt tttttttttt ttttttgaaa tggagtttcg 63300 ctcctgttgc ccaggctaga gtgcaatggt gcaatctcgg ctcactgcaa cctgcacctc 63360 ccaggttcaa gtgattctcc tgcttcagcc tcctgagtag cggggatcac aggcacctgc 63420 taccatgcct ggctaatttt ttgtattttt agtagagacg gggtttcacc atattggcca 63480 ggctggtcat gaactcctga cctcaggtga tccacctgcc ttggtttccc aaagtgctgg 63540 gcttacaggc gtgagccacc acacctggcc tagatttgct tttaaagagg tcttctgtat 63600 cccaagtata aggaggttct tgtgaaactg caatgcctct ttttcccttt cttttttata 63660 tttcagaata gctgcaatct ccatggcctc taagataaca gacatgcagc ttggaggttc 63720 agtggagatc agcaagttaa ttaccaaaaa ggaagttcat caagccaggt aatttttaaa 63780 atgcatatat gtgccttctt tcttcaatta gacttcaatt agagggcaga gattattcat 63840 ttatctcaat ccttcatagt agagccttgc atacacaata agtattcaat agacaatttg 63900 atgttgagac atctgctcca tccccaatga atcctactca ggagatggct aagactaaag 63960 gagacttttc caggcctcac agtaaccgaa ggaagcagag acctataggt gtttagctta 64020 acccatatga gaatagagaa cccagagagg tggccatctc tggagaggga gattgggctg 64080 ttaagagggg cacctgtgga gcatttgggg ctgagcagcc agggaacact ctgaggccac 64140 tggaggactc ctagacccca taacagagag gccatcatcc cacctgtcct tcagagggac 64200 ctcatccagc cattcctgga ttggggtccc tggggaaatg gtatttctag ggcaaggtta 64260 ctaatggaaa ctgaagagca ggtggcctca caggctgctt cctggcctga gggattgttt 64320 tttggcaaga gtcagctgtg cttccaggtt gactgagact tccttttctt gctggatggg 64380 agctgtacag tctgtattct gactttaatt ttttttcatt tgcttatcaa ggctggagtg 64440 tgatgaggag tgttcagcct tggaaaggaa aaagtaagta gttgcagctg cttttttaat 64500 ctccttgccc ttgagctctg tgaaattaat gggagggagc aacccttcaa acgtcattca 64560 ctcagcactg cgctgcgtct tttaccgtta tcaaagagca agagaaatgc cgttaccaag 64620 aattgaattg tcttaaggat aaaccactca ggtgatttca gcatattgag aaacatgagc 64680 tgtgtggttt tgggcatgat ataaacatca cataacacag catacttatt cagggactgg 64740 ccaacttcct gactcagaga taagaggttt aaaacaatgt ttagttaact tttcgttgaa 64800 gtttagcata catattgaag attggaagtg tgcagatcag tgaatcgtca ctaaatgatt 64860 acagttgtgt aatcatttgt gtcactaaat gaatacagtt gagtatggat acaggtgaaa 64920 agataattac cagaaccgtc tcttgtactc tacataaagg tgaaacctct atcctaattt 64980 ctctttagat tagtttttga actttatgta agtgatgaaa tcatgtagtt tgtattattt 65040 ttgtctggct tctttcgtta tatttttgtg acattcactc ctgttgttgc atgtagcaat 65100 gctttattca tttttattcc tgcatgattt tttttgtatt actattcatt tacccattat 65160 actgttaatg gatatctttg tattaactgt tagactatta caaataaaac tgttaagaat 65220 tctttttttt tttttttttt ttgagatgga gtttttctct tgtcgcctag gctggagtgc 65280 aatggcatga tcttggctca ctgccacctc tgcctcccgg gttcaagcga ttctcttgcc 65340 ttagcctcca gagtagttgg cattacaggt gtgtaccacc atgcccagct gatttttgta 65400 tttttagtag aaatggggtt tcaccatgtt ggccaggctg gtctcgaact cctgacctca 65460 ggtgatccac ctgcctcgac ctcccaaagt gttgggatta caagcttgag ccaccacgcc 65520 cagcccagaa cattcttaaa cttgcttgct ctatatttct gtacatttct gttggatata 65580 agccagaagt agaattgctg attaataggg tttatgtgtg ttcagcatta gtagattctg 65640 cccatgtcca aagtgattgt accaaattac actcccacca gctaaggata attcccattg 65700 ctgtacattt ttgtcagcac ttgaaattgt cactttttaa ttttagccat tttgtagatg 65760 tgtataacaa gatgtcattg agcttttaat ttctattttc ctgatgactt aagagattga 65820 gcacctttct tgtagttact ggccattgga tagcctcatt tttgaagtgc ctgtacaagt 65880 cctttgctta tttttttgtg ctctttttgt attgattagt aggaattctt tatgtattct 65940 gaatatgagt cctttgttgg ctctatgtat acattgtgag tactttgttg cactctgtac 66000 cttcattttt tactctcttg atgccttttt ttaatcccag aagctaaaga actcttgttg 66060 tcttttgata aacagaaatt tttaatgtta atattatcca gtttatttga gtttcttaat 66120 aagcactttt catgtcctat ttagaaaaat ttgtctatcc tgaggcaatg acaatattct 66180 gttattatct agaaactgtg ttattttacc tttcacattt taaatctaca gttgacctgt 66240 actgattttt ttgagtatgg ataaggagag tttacaattc agcttttttc cacgtgaata 66300 tccatttgac gagtaccaag attgtccttt tcccagcact ctgcagtgcc acctctgtca 66360 tacatcacat ataagcctgg gcaacttggc aaaacctcat ctgtacaaaa aataaaataa 66420 aattagccag gtgtggtggt gcacacctgt agtcccagct acttgggagg cttgaggtgg 66480 gaagattgct tgaacctgcg aggttgaggc tacagtgagc catgatcacc agtctggact 66540 atacttttgg tgacagagtg aaatgctgtc tcaaaaaaaa aaaaaaaaaa taagccgggc 66600 atggtggctc accatggccg taatggtggt gcctataatc ccagcacttt gggaggttga 66660 ggtgggcaga tcacaaggtc agcagatcga gaccatcctg gtaaaacccc atctctacta 66720 aaaatacaaa aaattagccg ggcatggtgg cgggcacttg tagtcccagc tactcgggaa 66780 gctgaggcag gagaatggca tgaacccagg aggcggagct tgcagtgagc cgagatggtg 66840 ccactgcact ctagcctggg cgacagtggg agactccgca tcaaaaaata ataataaata 66900 aataaagtat atcaaatata tatatatatg tgagtctgtt tctgagctca tcctgttcaa 66960 ctgcactagt atcacactgt cttaattact gtacctttat aacaagtctt aatatctggt 67020 agtatatatc ctcaaccatg acttttcttc aatattgtct ttgattattc tttattctta 67080 gccctttaca tttccactta atgtcttttt catttatgta tttatttatt gagataggat 67140 cttgctctgt cacccatgct ggaatgtagt ggcacaatca tagttcactg tacccttgaa 67200 ctcctgggct caagggatcc tcgtgcctca gcctccaaaa tagataggac tacaggcaca 67260 caccactacg cctggctaag attttctttt tttttttttt ttcttttgta aagacagggt 67320 tttactatgt tgaccagggt ggtctcaaac ttctggctca agcgaacctc ctgccttggc 67380 cccacaaagt gctgggatta taagcatgag ttactgcacc cagcccattt ccacttatat 67440 ttcacaattg gcttgttatt ataatatata cccaagaaac ctgctgggat tttgattgag 67500 ttggtattta acttgtaggt caatttggaa tctttacaat gttaaatttt ctagtccaag 67560 agaaggtgta ttcttgcatt tcatgtcttc tttgatttct ctcaatgata ctttatggtt 67620 ttctatatat aaattttgta ttctttcagt agatttgttt cttgcttttt gatgctgcta 67680 tagatgggtt tatttttctt gtattacttg taattttttt tttgagacag agtctcgttc 67740 tgtttcccag gctggagtgc aatggtgtga tctcggctca ctgcaagctc cacctcccgg 67800 gttcacgcca ttctcctgcc tcagcgtccc aagtagctgg gactgcaggt gcccaccact 67860 acacccggct aattttttgt atttttagta gagatggtgt ttcaccatgt tagccaggac 67920 agtctcaatc tcctgacctt atgatctgcc cgcctcagcc tcccagagtg ctgggattac 67980 aggcatgagc cactgcgcct ggccaacttt taattttttt taaattatta ttattattta 68040 atgtgacaag tgttttgttt attttgtttt tgaccaggtt gacatcctca aaaaaaaatg 68100 caaattacaa ggacttcatt ttaaagcaaa tgctcatttt agaaatgtgt tacagaagat 68160 atttatgtgc ttcagcaggc ataaaaatat atcacagata aggcaaaaaa attctgtgaa 68220 tggcttgatt tttttttttt tttttttttt tttttttttt tgatacagag tctcactctg 68280 tctcccaggc tggagtgcaa tggcacaatt acagctcact gcagcctcta cctcctgggt 68340 tcaagtgatc ctcccacctc acctcccaag tagctggaac tgcaagtgca tgccaccaca 68400 cccagctaat ttttacattt ttttgtacca cacccagcta atttttttgt ttttttttttg 68460 gtagagatgg catctcgcta tgttggccag gctggtctca cattcctggg ctcaagcaat 68520 cctcctgcct cagccttcca aagtgctggg tttacaggcg tgagccacca cacccggcct 68580 aaattggtat tttctaattg cttgttgctg gtatttagaa atacaattga taattattga 68640 cttatttagc aacctcgcta aattcactta tttctgatat tgtagattat tttggatttt 68700 gtatatacac agtcatgtct tctaataata gtaattttat ttattgtcaa ttcctgttat 68760 ttttttcctt tcttttttct tttatctttt ttccttcttt ccaattctta acttttattt 68820 cttcttctta cctcattgta ttggttaggg ccttcagtat attgttgaat ggagggtggt 68880 aataatagat attccttggc cgggcgcggt ggctcacgcc tgtaatccca gcactttggg 68940 agactgaggc gggcggatca cttgaggtca agagttcgag accagcctgg tcaacatggt 69000 gaaaccctgt ctctactaaa aatacaaaaa ttagccgggc atggtggcag acgcctgtag 69060 tcccagctac tcaggaggct aaggcaagag aattgcttga acctgggagg cagagggtgc 69120 agtgagccga gatggcgcca ccacactcta gcctagacga tacagcaaga ctcagtctca 69180 ccaaaaaaaa aaaatagatg ttcctgacta atcctccatc tcatggggaa aactttaagt 69240 atttcaccat taaatatgat gtctgcagta agttatttga ggatactcta tcagatcaag 69300 aggcatttat tcaacaactg acaaactttc tgattctagc aaagattgat taaattgaat 69360 agccctgcaa attgttaata attggagaat ttaggtgaag gttatataga agtttatggt 69420 aatattctca taacttgtct ttgaaatttg tttaagataa aaagttagac aaatgcagac 69480 aaagtatagc cctattttac cttgaagttt gactttcagc aattttaata gtacctcttt 69540 acatattaag tgtgtagaag gtaatatgat gactagtagt gctgtccaaa ttttccttgg 69600 tttgaccctt ataataactt tctagtgagt gccgttcaaa acccttgccc tttaggaacc 69660 gtcagtcagt gttctcagct gcagaatgta aattaggagt cctaggtatt cagaaatgct 69720 atagtgaata cacagaaggt ctccaaacat ggaaatgtac ttttggtggt tgaaagacat 69780 attgtatact agttagagcc agagctctgg agtcagattg tctggatttg aatcctggct 69840 tcacaaatca cttagctatg agaccttggg caagtcacat attgtctgta agccttaggt 69900 ttcttaaaaa tctagaaatg atgacaataa cagtatttac cttccagagt tgttgtgagg 69960 attaatacat taaaaatgat actcataaag ctcttggcac aagagcttgg cacatagtag 70020 gtactcagta aatgttattg attaaaacta gctatttttg aaagcctttt gaatttctcc 70080 tcaatcttgg tgatgacata atgaccctta ctggatagag tctttcataa atacacacaa 70140 acctaagaga aaaaaatgca aatttaaaga aaagtccatc taaagttata tctatatata 70200 cacacacata tatacacgca tacacctgtg tgtatatata tgtatgtgtg tatatctcta 70260 ttgtagaaat aaaaacctag caggccaaat tcagcccaca gacaggtttt attggtgttt 70320 aaatttgaat tagtggccaa ctaaccaaaa catcaaaaga tcttccccta gaaattctct 70380 tgaaaatttg aaaagtaagt gtcttttgaa aaattggaaa tctagcagtg tgaaccagat 70440 tcttgtgggc actaagcttg taagtgactc ttggtgcagt gtaaatgaaa attgtactaa 70500 gtgctaagct gaaatctagc tttaaaaaaa taaaataact tgaatgttgg tgagatgcca 70560 tgagattcag acactgatgg ctagggcact aagtggcatt tctggagacc acttactcca 70620 cattgatcaa gagtcttatg tccactccat gtaccccaca actggaaaca ttttcaaagc 70680 tttttgcaca gagatattaa ttgtgtcgta tataatattg agaaactgag gaactgttaa 70740 agaaacctaa atgggaaatg attaaaatat gggaacataa ttagaagact atgagccatg 70800 aagacagtta agtgtgaaaa caaaataata atatgagaaa tgctaacaag ataatgctga 70860 gaggcaaaga aacaaggttt ttttaatttt taaaattttt ttattaaata aaaatggtat 70920 atatttatgg taatacagca tgatgtgtat gtatacattg tggaatggct aaatcaagct 70980 aattagcata tgcattacct cacattctta tttttgggggg ggtgagacaa ttttgaatat 71040 aataatggcg gtacttaaaa ttatagagaa aggctgggcg cagtggctca cacccgtaat 71100 cccagcactt tgggaggtcg aggcgggtgg atcacctgag gtcaggagtt caagaccagc 71160 ctggccaaca tggtgaaacc ccatctctac taaagataca aaaattagct gggcatggtt 71220 gcgggcgcct gtaatcccag ctactcggga ggctgaggca ggagaatcgc ttgaatccag 71280 gaggtggagg ttgcagtgaa ccgagatcat gctattgtac tccagcctgg gcgacaagag 71340 cagcctgggc gacaagagca agactccatc tccaaaaata tatatatata cacatagaga 71400 gaaacgttat agtgaaaaaa ggggcccgtt acagcggctc atccccataa tcccagcatt 71460 tgggtggctg aggccagcgg atcacttgag ctcaggagtt caagaccagc ctgggcgaca 71520 tagcaaaacc ctgtctctac aaaaaataca aaaattagcc gggcatggtg gcacacttgt 71580 agtgccagct actggagagg ttgaggtagg aggatggctt aagcccagga ggtggaagtt 71640 acagtgagct gagatcatgc cactgcactc cagcctgggc aacaagagcc agacccagcc 71700 tcaaaaaaaa aaaaaaaaca attagaaaaa agggaacact tacacattct tggtgggatt 71760 gtaaattagt acagccacta tggaaaacag tatggaattt cctcacatta aaaatagaac 71820 taccatatga tccagcaatc ctattacttg gtatctctac agaggaaata aaatctgtag 71880 agatacctgt actctcatat ttgttgcagc actatttaca atagccaaga tatgaaatca 71940 actgaagtgg ccaacatggc tgcatggata aagaaaatgg ggtatatata cagaatgaaa 72000 tactattcgg ccataaaaaa aatgaaaccc tgtcatttgc aacaacatgg atggacctgg 72060 aggacatcat gttaagtgaa ataagccaga cacagaaaga cagacaccac acgatctcac 72120 tcatatgtag aatatttaaa aaataataca gaagcagaga gtagaccagt gttgtctgac 72180 tgagaagagg gacagggtgg aaaagggagg ttgaggagag gttggtcagt ggatataaag 72240 ttacaaatag atggtagtaa taagttcctg tggttttttt tttttttttt tttttttttt 72300 tgagaaggag ttttgctctt gttgcccagg ctgtagtgca atggcgctat ctcagctcac 72360 tgcaagctct gcctcccggg ttcaagcgat tctcctgccc cagcctcctg agtagctggg 72420 attacaggca tgcacaacca cacctgacta atttttgtgt ttttagtaga gacgaggttt 72480 ctccatgttg gtcaggctgg tctcgagctc ccagcttcag gtgatccgcc cgcctcggcc 72540 tcccaaagtg ctgggattac aggcgtgagc caccgtaccc agcctaagtt ctcgtgttct 72600 atggcacact ggggtgatga tggttaacgg taaggtgtag tattttacaa aatagctaga 72660 aaagaggctt ctgtgtattc tcaccacagt gaaataataa gggcatcagg tgatggatat 72720 gcctaataca ctgatttggt cattatacaa catagtatat atgtaccgag acatcaacat 72780 caaactgctt cataaatatg aagcaatgac aatgggtcaa taaaaataaa ataataaaga 72840 aatgtattat tattattatt attattatta ttattattat ttttagacag agtctcactg 72900 tgttgcccag gctggagtgt agtggcacaa tctcggttca ctgcaacctc tttctcctga 72960 gttcaagtga ttctcatgcc tcagcctcct agcagctagg attacaggca cgtgctacca 73020 cacccggcta atttttgtat ttttagtaga gacagggttt caccatattg gccaggctgg 73080 tctcgaactc ctggcctcaa gtgatccatc taccttggcc tcccagagtc ctaggattac 73140 tggtgtgagc caccgcaccc agccaagaaa tgtataaaaa tgaagtgtat atgttaatgg 73200 aattctttac ttttcagatt tctatattaa ttttaacatt tgtatgtcta ttttatgtac 73260 cttatcttta aacaaagcct cagagaaagt catcccccat tgaccattct cagtgatccc 73320 acacccccaa actcctcttc cccaaataat ctaagtcccc agtgttcagc ctatgacttg 73380 ctcagatcct tatagatctg tttattttct tttatgaaga caataccacc tcatgtccca 73440 agttgttgtt attctctcct taataatcca gtttaccaaa atagagataa ataatgtgta 73500 aagaatttag gatatagtag gcactcgggg ttactgcaag atagtttccc tcccactcct 73560 tttatttgca tacctctctc tctctttcag gagattagca gaggcatttc atatcagtga 73620 ggattctgat cctttcaata tacgttcttc agggtcaaaa ttcagtgata gtttgaaaga 73680 agatgccagg tatgtaactt gttacctgct ttcttaattg tggcttgtca gggtgtttgg 73740 tcttttgaga tgtcataact aatatgtact cctcctgtga ttaagccagt tcaagtttgt 73800 ttgtttatct attgtaagag attatgtgct gtttaataaa tatccttctc ttggtttctc 73860 gaaactgtcc ccttaaagaa ctgaacttgg ggtagggttg agataagagt ttgggtgggg 73920 tggtggtaag agtttgggcc tcccaagggc tggactagac taaattggag gatctctggg 73980 cttcattcca cttctgtcca ccccagtctc acattcacat gcgttctcag ttggagctat 74040 aacagctctt atttttctcc tggagtccct ggcctttcag tggctacttc tcctttcatc 74100 ttctcatttc ttatattttt ctcttttgtt cttccgtcag tcatccttct gttttctgac 74160 aattcctgat ctctgcagtt ttactgccag cattgtctat tgtctacgct ttacagggag 74220 aggatgggtg aaaggggttc tcagaagggc agggacagac aaaattaact ggtgatttct 74280 catttcagga aggacttaaa gtttgtcagt gacgttgaga aggaaatgga aaccctcgtg 74340 gaggccgtga ataaggttga agtcgaaaca tcccactgga catttctcta aggccagctt 74400 gatgaaaaaa aaaaagcaat caagtcctgg aaacactttc aacctagagt agttgtagga 74460 aaaagtcaca acttctttga ggatccttat ctttctcaaa acacagccat tataacatct 74520 gtggaagcct gtcctggttc aggacagttg actgcagaaa agatctacag tcggctgggc 74580 acagtgtctc atgcctgtaa tgccagcact ttgggaggcc aaggcaggca gatcacttga 74640 ggtcaggagt tctagatcag cttggccaac atgacgaaac cctgtctcta ctaaaaatat 74700 aaaaattagc tgggtgtggt ggcgggcacc tgttattcca gctacttgca aggcttaggc 74760 aggagaattc cttgaacccg ggaggcagag gttgctgtga gctgagattc tgccactgca 74820 ctccagcctg ggcaacaaga gtgaaactct gtctcaaaaa gaaagaaaga aagaaagaaa 74880 gctctgcagt cagctgtaag taaatgccag gcacggtggc tcacacctgt aatcccagcg 74940 ctttgggagg ctgaggcagc aggcggatca cttgagtcca ggagttcacg accagcctgg 75000 gcaacgtggc aaaaccctgt ctctacaaaa aataaaaaat tagccaggtg tggtggtgca 75060 cacccataat cccagctact cagggggctg aggcaggagg attgcttaag cccaggaggt 75120 caagcctgca gtaagctgag gttgagtcac tgtactccag cttggatctc cagcttgaga 75180 tcctgtctca ataaaaaaga aagaaagaaa aagaaaataa agatgctggc caagtgccta 75240 agaatggatg aagaaactct gggtgagggg gctcaatagt gatagttgag tgactgtgtg 75300 ggacacttag gaagacactc tagcccctca gatctttgaa gggctattgt atgctaggaa 75360 agcccactgt tctttataat tccaaaggca ggaatgtccc ctttgggaag gatgaaagtt 75420 gtatgactac agctggagag cagtgggata gactccttca gaagagggag acccctgtcc 75480 tggaggcggc cagggatgtc acggaggtag ctttaagtgg aaaagtcttg agtttagtca 75540 gaagccccca gcgctgcatt ggcttcctcc cccttccatc aggggggacg gtgagtaagg 75600 acaagatggt tgctttagcc cctgtatcta atgctggtgg ttttccttcc cagtctcctt 75660 accctcttcc cattcccctc ctttcctccc ttctgttacc acttcccagc agaatattaa 75720 catgaaggag aaaatggaat gcattattcc tctcctaatt aagaaacccc aacagatgtg 75780 tttctctgct tgtgtgtctg tatctgaata ctcacgattt ttaaagtaga acatactttt 75840 aaaggaaata atattttctt gttttgtaag tctttagtca tttttgggga atatcaccca 75900 ctccccagtt atggaaccca tcaggaagaa ccattatgct gtctacctaa gtcgcagttc 75960 acttaccctt tgtccctaac cctgtccagc tgcgactgtt gtggctcaca gagtaggtga 76020 aagaagtgca tcagtgccca tcttcccaaa cccctccagg cttgcttggc agttagtaaa 76080 atgctctatg ctgtgggcct ctaaaatcac ctcttattga tccctgcaat tcttgtgtgg 76140 taagattagt tgtaagcatt ttgttttcag aatgatatga tcaatcagtc atcttttccc 76200 tcaatacagg gaaagaatag taagaaaagc cacagcttcc ctcccatgaa cagagaccac 76260 cgccggatca tccatgactt ggcccaagtt tatggcctgg agagcgtgag ctatgacagt 76320 gaaccgaagc gcaatgtggt ggtcactgcc atcaggtagg tcaatcccgc cgtcagagga 76380 agaactccta agcagggcaa actggaccca agaagcaggg tctgtcaata cttgtctttc 76440 ttactaccac tctccctctt ggaaaagtag aaggaaaatc tgcttctctg gcacttccca 76500 gataatctaa aggcctgagc caaagagcaa aacctatgcc tcgacccaca gggcagatgc 76560 actcaaaatg cacaagtgtc acatctgcct acagtcatag cagatgctgg aagcagcatt 76620 tcttcaggca agggggcctg cccatcatat ctgctctttt ctccttctgt ctcctgttcc 76680 tctgtctgtc ctctccttgg ctctgtcctc ttggcatggg gaaaacagaa agaatgctgg 76740 actctggttc gaggcatccc ctgtgaatgg tgtttttgcc atcagaggaa actgaagcaa 76800 taatgtgacc aggaggggac agggaggacc taggatcact ctcagaacct gggaatagga 76860 agaacttgcc agtgaagaaa acaagtctga catgaagcaa gccacgggcc tggcataggt 76920 gggtggaagg gacagggggt taaatgcagg tggccatgtc tgagagcaga cagttcatac 76980 cagagagtgc tcagtagtgt catagtgcca aaatcaaggg ctgagtttct agctttctgt 77040 ccatctccac aaacaattct cacttttcaa tgcttggtgt tttgactttt atcaggggga 77100 agtccgtttg tcctcctacc acgctgacag gtgtgcttga aagggaaatg caggcacggc 77160 ctccaccacc gattcctcat cacagacatc agtcagacaa gtaagattct ccagctgctt 77220 tccaagggga cctgtctgtc cagaaaagct agcaggggct gaattgtcct gagctgcacc 77280 agccattggt ggcctcctca ggcctggcct tggggtacca tgttcaaata tgtaatgttt 77340 agttagggta tcagacacgt ttgttcccac cagaggggtc ctaagtttgt tgtttgtagt 77400 gcttgattct agagagagca gtcactggac ataatttcca tggtgtagat ggtgacactg 77460 ggaagtggat tcagtctgtc ataccagata gtttaagact gaaacatcgt gtagttagga 77520 atagttactc tgggccgggc gcggtggctc acgcctgtaa tcccagcact ttgggaggcc 77580 aaggcgggtg gatcacttaa ggtcaggagt tcgagaccag cctggccaac aaggtgaaaa 77640 cccatctcta ctaaaaatat aaaaaagtag ccgggtgtgg tggcgggcac ctgtaaacac 77700 agctacttgg gagactgagg caagagaatc acttgaacct tggaggcaga ggttgcagtg 77760 agccgagatc gcaccactgc actccaccct gggcgacaga gagacagact ccatctcaaa 77820 aaaaaaagaa tagttactct gaagatgcaa gtgggtgtgg gggtgagaat tgccggtgac 77880 gggggtggat agtactttct tgttttgctc tgtctgcttc tatatggttt gaataatttt 77940 tataacaaac atattcatga tttaaagaga aaagaaaacc aagttggcaa ggggcaagtc 78000 tgccagatgt tttctcagac aggagcaggg aaggagctct gagagttggg tgactgttgg 78060 gctttatttg gagaaaccag aattgtgggc tgaaaccaac tttagagcac ttggtcatag 78120 ccccagttgt cccagttgtt tgtcacttgt cagggagagg aagggaaggc aggatggtgc 78180 tccttctctg ctgccctagt gtaaccaagg cagtgtggct aaaagttact tcaaagccca 78240 gaaggagagg ggtgcctact cctgttctgg ctttccacag acaagtccta gcagtacctc 78300 ctctgtgatt cctgcacaaa acctctgaag ggtggttacg gcagttcaca aggtcaccat 78360 gaaggtcaca gcacttagtg ttcctgtggg actggacaag aagacagcta ggtgttgccc 78420 attgagctca gactcagcca agagcgtcct ggtccccagg accagcccct ctccactcct 78480 gggagccagg tgtcctaggt tctggtcagc acagtctgca aatccatatg ggcggtcact 78540 ttggaaacat gaggggtttt ctcagaaact gccagagtcg ggtccagtcc tgcttagaca 78600 caaccatgtg gagttattgg cccgtttatc ccatgctatc ttttttcttt ttttcttttt 78660 ttttgagatg gagtctcgct ctgccgccca ggctggagtg cagtggcgcg atctcagctc 78720 actgcaagct ccgcctcccg ggttcatgcc attctcctgc ctcagcctcc tgagtagctg 78780 ggactacagg cgcctgccac cacgcccggc taattttttg tatttttagt agaaacgggg 78840 tttcaccatg tgttaggatg gtctcaatct cctgacctcg tgatccgtcc gccttggcct 78900 cccaaagtgc tgagattaca ggcgtgagcc accacacctg gccatcccat gctatctttt 78960 taaaaaccct tggcttgctt tatttttgta taaaactcta ttatggaaat gttttagagt 79020 agagagaata gtattactat cataaatgcc catgtgccca ttaccaaatt tcaaaatcac 79080 cagctcatgt ttgtcccact atcaaaactt cacaagtgag atgctcgctt ggcttaagct 79140 gttaactgag cattcattga actgtgaagg cagagtaaaa tcatcatcat atatgaaatc 79200 ctgtaaatag attgtgaaag gttacctcta aactgtaaag tgtaaataga gaatttgtta 79260 tctcacagaa taaaattccc catgtaatat gtaaatctag aaggaaacac aaaagttaaa 79320 aaaattgctc acaaagggca gaaggaaaaa aagtaagact aaaataaaaa taaaataaat 79380 gtttaagatt cacaaatggt aaatcagctg atccttaact ttctgaagtc ctgtatctgt 79440 ttcccccaaa gaagaaaaga ctgatcattc tttgtttgtt tttcaggaat cctgggagca 79500 gtaatttaca gaaaataacc aaggagccaa taattgacta ttttgacgtc caggactaag 79560 aagatcatga tgcacttaga taaaagaatg attaggtata gtggagactt atttgccagc 79620 agataaatca tgcccgttcc cctctgcctg gcagaatcac agtctcacat actgtcttgt 79680 actgacacat ccaaagcatg agtgtgtcag aaatcccttg tctattcctg tctgtataaa 79740 gtgtttcatt atgaccagat ctctgattgt atggtcacta ggtatgcaat cacgcattca 79800 aagaggctct ttacaccatc actgtgattg ctctgagagt tgagggacta ttgggcttta 79860 tttggacaaa ccaaactttt agcctgaaac caactttatg ccactaagtc atagcctcag 79920 ttgtcccagt tatttgtcct cctgaaaatg cctgaaacat cagacagaca ttgcttgctt 79980 tacccaaact gatcaaaatc tttaggagca caaatgaatt ttttagtctg aaataccaaa 80040 taatgaattg gtataccata tccggaatca cacatgttat cttaaaccca gccatcatac 80100 ctaagtcttt tgccaaaacc tctcataggt atatctagct gaacttattt tggcattttc 80160 aatgtgatca gttctagacc tagaaggggg tcaggctgct ttacagaatt ctatttcctt 80220 aagtccctgg cacttctcat accacatcac tgaacctgtt cagtaacaat cagtttggcc 80280 gtcccccatg atggtaggaa atatagagag caagttcttc tgccagggtc acactgtggt 80340 ctctgaactg accagtatat ccctaactcc tctttgatag agaaagagtc tcaaatggac 80400 aactgtcctg tgttgctttc cctaggcctt cagcagccta ttggctctcc ctgcctctga 80460 gctctggact ctgtttgaat attccaagta gtatatggac agtccagggc ttatgcccag 80520 cagcccactg gaggcattct tcaggctcct ttaaggcagg tgcattgata gttccattag 80580 tgtgaccctt gcattggcac ccctccagcc tggaggccag gcttccagca acttccttct 80640 gccctagagc aagccatgag ccccagagca gtagcaggag acttgagaag tagagtgaca 80700 aaaacaagca cttaattaaa ttataaaatt taacttta 80738 <210> 2 <211> 1104 <212> PRT <213> Homo sapiens <400> 2 Met Glu Phe Ser Ser Ile Cys Ile Glu Phe Lys Ser Thr Leu Arg Gln 1 5 10 15 Glu Ala Pro Pro Ser Arg Ala Ala Glu Pro Arg Ser Ser Cys Thr 20 25 30 Val His His Leu Pro Val Thr Phe Pro Gly Arg Ser Leu Met Met Lys 35 40 45 Ser Leu Leu Phe Ile Ser Ile Val Ile Ile Arg Gln Glu Gly Lys Pro 50 55 60 Lys Ser Gln Gln Thr Ser Phe Gln Ser Ser Pro Cys Asn Lys Ser Pro 65 70 75 80 Lys Ser His Gly Leu Gln Asn Gln Pro Trp Gln Lys Leu Arg Asn Glu 85 90 95 Lys His His Ile Arg Val Lys Lys Ala Gln Ser Leu Ala Glu Gln Thr 100 105 110 Ser Asp Thr Ala Gly Leu Glu Ser Ser Thr Arg Ser Glu Ser Gly Thr 115 120 125 Asp Leu Arg Glu His Ser Pro Ser Glu Ser Glu Lys Glu Val Val Gly 130 135 140 Ala Asp Pro Arg Gly Ala Lys Pro Lys Lys Ala Thr Gln Phe Val Tyr 145 150 155 160 Ser Tyr Ala Arg Gly Pro Lys Val Lys Glu Lys Leu Lys Cys Glu Trp 165 170 175 Ser Asn Arg Thr Thr Pro Lys Pro Glu Met Leu Asp Pro Lys Val Pro 180 185 190 Asn Leu Trp Gly Phe Ser Thr Leu Thr Leu Gln Arg His Pro Leu Glu 195 200 205 Lys Glu Tyr Trp Met Gly Met Glu Pro Asp Glu Met Ser Arg Glu Asp 210 215 220 Thr His Arg Lys Gly Leu Pro Gly Lys Trp Arg Gly Pro Gly His Asp 225 230 235 240 Gln Ala Glu Ile His Gln Asn Arg Arg Ala Thr Asp Ile Gln Thr Gln 245 250 255 Asp Thr Glu Thr Thr Trp Ala Pro Phe Gln Ser Asp Asp Leu Asn Glu 260 265 270 Arg Pro Ala Lys Ser Thr Cys Asp Ser Glu Asn Leu Ala Val Ile Asn 275 280 285 Lys Ser Ser Arg Arg Val Asp Pro Glu Lys Cys Thr Val Arg Arg Gln 290 295 300 Asp Pro Gln Val Val Ser Pro Phe Ser Arg Gly Lys Gln Asn His Val 305 310 315 320 Leu Lys Asn Val Glu Thr His Thr Gly Ser Leu Ile Glu Gln Leu Thr 325 330 335 Thr Glu Lys Tyr Glu Cys Met Val Cys Cys Glu Leu Val Arg Val Thr 340 345 350 Ala Pro Val Trp Ser Cys Gln Ser Cys Tyr His Val Phe His Leu Asn 355 360 365 Cys Ile Lys Lys Trp Ala Arg Ser Pro Ala Ser Gln Ala Asp Gly Gln 370 375 380 Ser Gly Trp Arg Cys Pro Ala Cys Gln Asn Val Ser Ala His Val Pro 385 390 395 400 Asn Thr Phe Ser Cys Phe Cys Gly Lys Val Lys Asn Pro Glu Trp Ser 405 410 415 Arg Asn Glu Ile Pro His Ser Cys Gly Glu Val Cys Arg Lys Lys Gln 420 425 430 Pro Gly Gln Asp Cys Pro His Ser Cys Asn Leu Leu Cys His Pro Gly 435 440 445 Pro Cys Pro Pro Cys Pro Ala Phe Met Thr Lys Thr Cys Glu Cys Gly 450 455 460 Arg Thr Arg His Thr Val Arg Cys Gly Gln Ala Val Ser Val His Cys 465 470 475 480 Ser Asn Pro Cys Glu Asn Ile Leu Asn Cys Gly Gln His Gln Cys Ala 485 490 495 Glu Leu Cys His Gly Gly Gln Cys Gln Pro Cys Gln Ile Ile Leu Asn 500 505 510 Gln Val Cys Tyr Cys Gly Ser Thr Ser Arg Asp Val Leu Cys Gly Thr 515 520 525 Asp Val Gly Lys Ser Asp Gly Phe Gly Asp Phe Ser Cys Leu Lys Thr 530 535 540 Cys Gly Lys Asp Leu Lys Cys Gly Asn His Thr Cys Ser Gln Val Cys 545 550 555 560 His Pro Gln Pro Cys Gln Gln Cys Pro Arg Leu Pro Gln Leu Val Arg 565 570 575 Cys Cys Pro Cys Gly Gln Thr Pro Leu Ser Gln Leu Leu Glu Leu Gly 580 585 590 Ser Ser Ser Arg Lys Thr Cys Met Asp Pro Val Pro Ser Cys Gly Lys 595 600 605 Val Cys Gly Lys Pro Leu Pro Cys Gly Ser Leu Asp Phe Ile His Thr 610 615 620 Cys Glu Lys Leu Cys His Glu Gly Asp Cys Gly Pro Val Ser Arg Thr 625 630 635 640 Ser Val Ile Ser Cys Arg Cys Ser Phe Arg Thr Lys Glu Leu Pro Cys 645 650 655 Thr Ser Leu Lys Ser Glu Asp Ala Thr Phe Met Cys Asp Lys Arg Cys 660 665 670 Asn Lys Lys Arg Leu Cys Gly Arg His Lys Cys Asn Glu Ile Cys Cys 675 680 685 Val Asp Lys Glu His Lys Cys Pro Leu Asn Cys Gly Arg Lys Leu Arg 690 695 700 Cys Gly Leu His Arg Cys Glu Glu Pro Cys His Arg Gly Asn Cys Gln 705 710 715 720 Thr Cys Trp Gln Ala Ser Phe Asp Glu Leu Thr Cys His Cys Gly Ala 725 730 735 Ser Val Ile Tyr Pro Pro Val Pro Cys Gly Thr Arg Pro Pro Glu Cys 740 745 750 Thr Gln Thr Cys Ala Arg Val His Glu Cys Asp His Pro Val Tyr His 755 760 765 Ser Gly His Ser Glu Glu Lys Cys Pro Cys Thr Phe Leu Thr Gln 770 775 780 Lys Trp Cys Met Gly Lys His Glu Phe Arg Ser Asn Ile Pro Cys His 785 790 795 800 Leu Val Asp Ile Ser Cys Gly Leu Pro Cys Ser Ala Thr Leu Pro Cys 805 810 815 Gly Met His Lys Cys Gln Arg Leu Cys His Lys Gly Glu Cys Leu Val 820 825 830 Asp Glu Pro Cys Lys Gln Pro Cys Thr Thr Pro Arg Ala Asp Cys Gly 835 840 845 His Pro Cys Met Ala Pro Cys His Thr Ser Ser Pro Cys Pro Val Thr 850 855 860 Ala Cys Lys Ala Lys Val Glu Leu Gln Cys Glu Cys Gly Arg Arg Lys 865 870 875 880 Glu Met Val Ile Cys Ser Glu Ala Ser Ser Thr Tyr Gln Arg Ile Ala 885 890 895 Ala Ile Ser Met Ala Ser Lys Ile Thr Asp Met Gln Leu Gly Gly Ser 900 905 910 Val Glu Ile Ser Lys Leu Ile Thr Lys Lys Glu Val His Gln Ala Arg 915 920 925 Leu Glu Cys Asp Glu Glu Cys Ser Ala Leu Glu Arg Lys Lys Arg Leu 930 935 940 Ala Glu Ala Phe His Ile Ser Glu Asp Ser Asp Pro Phe Asn Ile Arg 945 950 955 960 Ser Ser Gly Ser Lys Phe Ser Asp Ser Leu Lys Glu Asp Ala Arg Lys 965 970 975 Asp Leu Lys Phe Val Ser Asp Val Glu Lys Glu Met Glu Thr Leu Val 980 985 990 Glu Ala Val Asn Lys Gly Lys Asn Ser Lys Lys Ser His Ser Phe Pro 995 1000 1005 Pro Met Asn Arg Asp His Arg Arg Ile Ile His Asp Leu Ala Gln Val 1010 1015 1020 Tyr Gly Leu Glu Ser Val Ser Tyr Asp Ser Glu Pro Lys Arg Asn Val 1025 1030 1035 1040 Val Val Thr Ala Ile Arg Gly Lys Ser Val Cys Pro Pro Thr Thr Leu 1045 1050 1055 Thr Gly Val Leu Glu Arg Glu Met Gln Ala Arg Pro Pro Pro Pro Ile 1060 1065 1070 Pro His His Arg His Gln Ser Asp Lys Asn Pro Gly Ser Ser Asn Leu 1075 1080 1085 Gln Lys Ile Thr Lys Glu Pro Ile Ile Asp Tyr Phe Asp Val Gln Asp 1090 1095 1100 <210> 3 <211> 40335 <212> DNA <213> Homo sapiens <400> 3 ctgggtgaga agccatggcc agagcttctc ccaggcacag gtgttgcacc agggacttct 60 gcttcaagtt ttggggtaaa gacacctgga tcagactcca agggctgccc tgagtctggg 120 acttctgcct ccatggctgg tcatgagagc aaaccgtagt cccctggaga cagcgactcc 180 agagaacctc ttgggagaca gaagaggcat ctgtgcacag ctcgatcttc tacttgcctg 240 tggggagggg agtgacaggt ccacacacca cactgggtca ccctgtcctg gatgcctctg 300 aagagaggga cagaccgtca gaaactggag agtttctatt aaaggtcatt taaaccactg 360 aagggaagat gctcagggcg agtgggtgtg tgtgtgtctt ggtgtgagtg tctgcaggga 420 gagaggtttt aagagccctc tttttttttt tttttctttt tttgagacag agtctcactc 480 tgtcttccag gctggagtgc aatgacgcta tccccgcttt ctgcaacccc cgcctcctgg 540 gttcaagtaa ttctcctgcc tcagccttcc gagcagctgg ggctacaggc acccatcacc 600 acgcctggct aatttttgtt tttttattaa agaaagggtt tcaccaggtt ggtctggctg 660 gtcttgaact cctgacctca gatgatccac ccacctcggc ctcccaaagt gctgggatta 720 caggcatgag ccaccgtgcc cggccaagag ccctcttttt agagccttta agaaaggtca 780 tgctagcctc tcgacccctg agtagctggg attataggta tccatgcctg acaccattttt 840 gtattttttg ta gaaacggg gtttcatttc accgtgttgg ccaggctggt ctcgaactcc 900 tgacctcagg tgatccatcc gcctcggcct cccaaagtac tggattacag gcgtgagcca 960 ccatcccagg cctgaaagtc ttttaaaaaa gatttttgag gccaggcaca gtggctcaca 1020 cctgtaatcc cagcacttag gctgaggcag gcggattacc tgaggtcagg agtttgagac 1080 cagcctgggc aacgtggcga aaccctgtct ctactaataa tatgaaaaaa tcagccggtt 1140 gtgttggcat gcgcctgtaa tcccagctac tcgggaggct gaggcaggag aatcgcttga 1200 acccaggtgg tggaggttgc aatgagccaa gattgggcca ctgcactcca gtctgggtga 1260 cagagcaaga ctccatctca attaaaaaaa aaaaaagatt ttttagtctt ttcattttca 1320 aaattgactc attgtaaatt caaacagcac aaatcttaat ctttctgtca tcctttcccc 1380 ccagctgctc agttcctctc tctgaaagcc accatgtcta atctgttttt tgaacgttct 1440 tgagctggtt tgtgccgaca caagcctgtg tgtgtgtttg tgtatgttag ggttattttt 1500 gtttttgttt ttgagatgga gtctcactct gtcgcccagg ctggagtgca atggcatgat 1560 ctcggctcac tgcaacctct gcctcccggg ttcaagcaat tctcgtgcct cagcttcccg 1620 agtagctggg attacaggca cccgccacca cgcccagcta gtttttgtat ttttagtaga 1680 tatgaggttt caccatgttg gccacggtgg tctccaactc ctgacctcag atgatccacc 1740 tgcctcggcc tcccaaagtg ctggcattac aggtgtgagc cactgtgccc ggcatagggt 1800 tgtttttaac tgttaacttt tttctgatta caaactcatc ttcaccatga aaaagtcaaa 1860 caaaataggt aacatggggg gccggatgct gtggctcaca tctgtaatcc cagtactttg 1920 ggaggctgag gcgggtggat cacttgtggt caagagtttg agaccagcct ggccaacatg 1980 gcaaaacccc catctctaca aaaaaataca aaaattagcc aggtgtggtg gcaggtgcct 2040 gtaatcccag ctacttggga ggctgagctg ggagaatcac ttgaacctgg gaggtggagg 2100 ttgcagtgag ccaagattgc accactacac tccagcgtgg gtgacagagc aataacctgt 2160 caacaacaac aacaacaaaa agtaatatgg accgggtgca gtggctcatg cctgtaatcc 2220 cagcgctttg ggaggctgag gtggtaggat cacttgaggc caggaattct caaccagcat 2280 ggccaacatg gcaaaaccct gtctctatta aaaatacaaa aataaggtgg gctttatggc 2340 acatgcctgt aaacccaact actcaggagg ctgcagcacg agaatcgctt gaacccggga 2400 ggcagaggtt gtagtgagtt gagattgtgc cactgcactc cagcctgggt gagagagcaa 2460 gactcttgtc ttaaaaaaaa aaacaaaaac gtaagaaaga aaggtctcct ggagttgtac 2520 atcagtgtgc tctgcaccca ttccc aataa cactcagcac tgctaggaga gttttgtcat 2580 cccgagggtt gaaacgagga ttcagccatc tcaatgagaa acacatgtta atgggttgaa 2640 acatatctcc aaactgagat ttcctctact tgctgggcaa gtatgcagat gtgtttcaga 2700 gataaagccc agctgctggg cagagtacaa gactaaatgc ctgggaatgg attcagaggt 2760 tcctacagca gtccaggcaa gtgaccagga cctagaccaa tgcttgcagg gaaatttagg 2820 gaagaggagg gagtgggcag gggagatgtt taggagagag aacatccagg ctttcacctg 2880 acgggcaaga ttctgctaaa aaggaaaagg aaggctgggc gtggtggctc acacctgtaa 2940 tcccagcact ttgggaggct gaggcgggtg gatcacctga ggtcaggagt ttgagaccag 3000 cctggccaac atggtgaaac cccatctcta ctaaaaatac aaaaattagc caggcatggt 3060 ggtgcacgcc tgtaatccca gctagtgggg agactgaggc gggagaattg cctgaggcgg 3120 aggttgcggt gagccaagat ggtgccactg agctccagtc tgggcaacag agtgagagcg 3180 agactctttc tcaaaaaaaa agaaaaaaaa aaaaaaagag gaaatggaat ttatagttcc 3240 tgccctcaga gaatggaagt ggaatttaga gttcctctcc ttagggagtc cagagattgt 3300 ggaggaggaa acaatccgac ccagtaccag gcagctagca gctgaggccc caaggcacgc 3360 tacatcccag acagggccct gtctacaccc tgaggctgag tctgcgtgtg gccccacagt 3420 ccacagtcag acccgcgggg cactaggcta ctcagagggc agtgtgggca ggacagctcc 3480 ctttggccat cgtcatgaga ctgataaacc agtcacttct atatcagagt actgatttgc 3540 ctcttgaggt tgtttgtggc aataaattga ggttgttcta ccatggttat agagcagcac 3600 catcccacag aattacagtt tgacccacaa atgcaagcta cttatgtaat tttaaatttt 3660 ctagtagcga catttttaaa aagaaacagg tgcaattaat cttagtaatg tatttaaccc 3720 aaatgaccca aaatattatc acttcaacat gtagtcagca tataaatagt aatgggctaa 3780 tagccctgca ctgtggcagg tacctgtaac ccggcatttt gggaagccga ggcaggagaa 3840 gattgcttga ctccaggagt ccaggagttc aggacaccag cctgggtaac aaagtgaaat 3900 gcttccccat gtctacaaaa gataaatact aggcatggct gcagcatctg tggtcagcta 3960 cttgggaggc tgagtgaggt gggaggatta cttgagccca ggagttcgag gctgcagtga 4020 gccgtgattg caccactgca ctccagcttg agtgagagtg agactctggc tcaaagatag 4080 attttttttt tcttttttga gacggagtct ctgttgccca ggctggagtg cagtggcatg 4140 atctcggctc actgcaacct ccaccgcccg tgttcaagcg attctcctgc ctcttcctcc 4200 cgagtgcact atcatgcctg gctaattttt gtattt ttag tacagacggg gtttcaccat 4260 gttggccagg ctggtcttga actcccgatg cacccgcctc gccctcctaa aatgctggaa 4320 ttacaggctt gagccaccgc gcccggtcga tatttttttt tcctaaaaat tgagatagtt 4380 taacagcctt ttttgtccta agtcttatag cgcatctccg tttgcactgg ctcaggactc 4440 gagagccaca cgtggctagt gcctactgtt ctggcgcgct caggcttgga gccttctctg 4500 cggaggccaa gtcctgcaag ggatctgttt gctgcggttt cctagtagtg tctgccgcat 4560 cactggggcc cggtgatctg ggtaaccatg tgagcttggg taacctctcc tctccctctc 4620 ctgggactgc tgtggacgtt aaatacagcg gtggatgtta gagacgcagt tcagtcactg 4680 gcattgattc gccgttgttg ctgctcttct ttcctcaaag gcgactgaag ggcagcaggc 4740 ccatgctcgc ttctgtctgc ccgatgagtc acttcaccct aggcctggcc ctctagagaa 4800 cactcctcta agattacaga atgcaaatct ggattccaga gctggcagag gccccagggt 4860 caggctctag gacaaccggc gaaaaagcgg gaacctctac tccacgccct tggtttttcg 4920 ccccttgaca gcttgaggct ccgccccgct ttgccccgcc cccccagctc cggccgacgc 4980 ccaccggaac tacagccccc atgatgcagt gggagtccga gcctctgcgt cgtccgcttg 5040 ggacgcgccg gcggaggagt ggcgcgcgga ggagtggcgc g ctgagacgc cgctcgaagc 5100 gccgagtcgc ggggcagcag aggcgtaagg agtaggcggg gcgagccggc tgggctcagg 5160 gtccaccagc tcacccgggt cgaggggcaa tctgaggcga ctggtgacgc gcttatccac 5220 ttccctcctc ccgcctcccc ctggggtggc gctcgctggt gacgtagtga gtgtgatggc 5280 cgccgcgagg ccgggaaggt gaaggtgaga gggcgagcaa gccggggtgg cggggactgg 5340 cccggcccgg cccggcgagt cctgagcagc tcggcgtggg ccgacgggat tctggggcca 5400 cgggggtctg ggatgcagtc aagcggggtc cccactctcc ggcctcgccg ccgggccctc 5460 cccggccctg agaggagggg gcgggccacg ggacctggga ctggtcggtg gggtcccctg 5520 tagcttctca tcgcccccgc ctttccaccc ttcacggcca tgttctaccc ctgccccttc 5580 tctatttttc atcctgtctt cattttgcca gcatttactc agcacctgat aggtgtccac 5640 gttcaccgcg ggagcgccct gccatcccca ctttgggcag ttactgcctt ctcgttcagc 5700 cctgtttact tatatgactt ctcaccggac tgtaaggccc tggagggcag gactgtttct 5760 tatccatttc taacgtggcg cctgacacac acagagaagg cacacagaaa atgttgaata 5820 aatagctgtt aagacgcacg aaggcagaac tgcccgttac cccctctcgt aatccttcat 5880 tgccctcttt aaattctcaa tcatctctct ctcttctacc cagaaaa ggg ctctggttat 5940 ctcttttcta cccagaaaag ggctctggtt atccgcctct ggtgtcccaa ggtttggggc 6000 ttgggggagg aaggcagctc aatgtagtta aaaaaggaag gaggccggtc gcagtggatc 6060 acacctgtaa tcccagcact ttgggaggct gaggcgggcg gatcacctga ggtcaggagt 6120 tggagaccag cctggccaac atggtgaaac cccgtctgtg ctaaaaccaa aaactagccg 6180 ggcttgctgg cacacgcctg taatcccatc tactcaggag gctgaggcag gagaatcgct 6240 tgaacccggg aggcggaggt tgagccgaga tcgcgctgct gcactccagg agctttggag 6300 tcaaagggag ctaatatttc agatcccagc ttagacaaat cttcaagtat tccagccctc 6360 tgtcccacag aatagagaat taagtgagat actttacagt agctgaacac attggctttc 6420 agtcagttct tctgttcctc aatagtcagc tgttccacag tcctcaagcc cagttttgtt 6480 cctagttttg ctctgtcctc ttctcccctc tctggatgta cctttcccag ggctcctgca 6540 gggacctccg tggtgcctca aacccgcaca tggcggaata cgtgtgcaca gtcaggattg 6600 gtcccacacc tctgcgtttt tttccccagg gcacttcctc acatgccagc gcaactcccc 6660 aatacctcaa tgagtaagct tccctcaggg atgagtttgt ttggcttgaa acctgattag 6720 acatgttttg tacaccagtg tttttttttt ttggacaccg gatccatctc cc ttttcttt 6780 tctaataagt caggactggt ggagtcaaca cagtcaatca atagccaacc tcaacctgag 6840 acaggacaga agagaactca gaatcttttt gtcttttgga cttcagccat gtccatgatg 6900 cctaccctgt gaagatctct caccatccaa aaaagtaagt agtagctgtg acttctggca 6960 ccctggattt gagctgagtg gacaggggtg tgctctcctg gacctctggt ggcaagtctc 7020 ttgaatggca tcctctttgt atagagccac agctttctaa cttgtgttga aaaactaccg 7080 tatcttttac ttccgtgaag gatttcgctg gttgcttaga gagcctgttt aaaagttgac 7140 agttcagaag tggctgggat ctcttgggct ccctgaagag ttagtctctg tctccctcat 7200 ttggtctctg ctgtgctggc tcttccccat ctcggcccag cacaggttgg ctccttgtca 7260 ctgctctttt aagactctgc tctgtcccct ttaccctgta tactcttttt gtttcgattt 7320 ctcagcttct cctcttttcc agttgtgcca gcagacgatc cgtggatcca ttttattttc 7380 cctgctagga gttgctgtac ataacactgg gtaaggaggt ggtgtggatt cagggactgt 7440 gcttgggatc caaacacacc ttgcagtgaa ttttgctact gctgtctcta cacccaagat 7500 acctaatact cctcaacgaa tagcctaggt tgaggcaaag ctgttgacca ccttggaggg 7560 ctaatagcag tttccattta gaactgggtt catttctggt cttaggttaa tatttatt aa 7620 tactgtgccc tggcactttt taggggtagg ggcaggagag taccagaaag agtgggcttt 7680 tccctaaagt gtgatcctcg ggattttaaa agctattaca cagaagggtt ttttcattga 7740 ttatatttgt gaaatgctga attcaacaca gttaaactag tgtctttaca acaaggcttc 7800 cttcccaaac tcttttgctg tgctcacgtg ctccactgag gaaaatggta gcatatggtg 7860 ttttttgttt gtttgtttgt tttttgtttt ttttgagaca gagtcttgct ctgtcaccca 7920 ggctggagtg cagtggcgcg atcttgcagt ggcgtgatct tggctcactg caagctctgc 7980 ctcccaggtt cacactatcc tcctgcctca gcctcccgag tagctgtact acaggtgcat 8040 gctgccatgc ctggctaatt ttttgtagtt ttagtagaga tggggtttca ccgtgttagc 8100 caggatggtc tcgaactcct gaccttgtga tctacccacc tcggcctccc agagtgctgg 8160 gattacaggc gtgagccacc gcgcctggcc tagcatatgg tgttttacaa actgattcta 8220 ctattgaacc ttctttacat gggatattgg gtgcaagtcg agttctgtgg accacgcttt 8280 gggaagtgct gccagaaact atccaaaaca gtaatctcca gagcacttat gaggcagtca 8340 cagcttgaag tgtgacgctt agtgtgtcac tgatgttgga gctgggctct caggctatgc 8400 ttgcaggatg gtccccacgt gcctgtggca aggtctgtgg tgaagcagag attatcaagg 846 0 ctttccagag gcagaggagg aacccagctg ccctgcatca ccctggagga gtagggagtc 8520 agcagtcaaa gccttgagat aattggggaa cacaggatgt gataaaaacc agaagtctgt 8580 gatgttgttt gttctctatg aggcatcaga ggaggcttca tggagcgggt gacacttgag 8640 ttgggtctag aaggacagta ggagtatgcc aaatgggtgg aaaaggaagg aggattccag 8700 gcagaaagca gctcatgcaa cacagatgtg gaatctcagc ttgcaagtta gcagagccag 8760 ataaatcagg cagagtttgg aggggttgac agtaggtttt acgccagggg tttggatgca 8820 gctgcaggct gggtattagt gagagcaggg aggctcctgg accggctgaa ctcccagcag 8880 accttgtaac ctggagctcc caagcttcta ctggtgtaca cgctgtcgag tcttgcagtt 8940 agtgagcact aatgcttttg gttttatatc tcctgggctt gcaaacattg accagaactt 9000 aaaagcacct gcttctgatg gcagggaact tgcttcctgc agcaagtcac ttctggagtg 9060 gggtatcgct gatccttccc cagctgacct gtttatttga tctccgtgat agcgggtaag 9120 ggagtggtgt gtgtggtgtg agttggcgga gttgcctctg ccctccctga aggctgggtg 9180 ttcatggaga gagtcttgtg atcttgctgg tagagtttgt tgttattgtt ttataacatg 9240 taaagctgcc tttagctctt actgtagtga agcatgttgc ttgcgtcatc aactttagtc 9300 ttct cacagt ttagatggta ttgtggtatt aggatattgg ttttcatcca cggatccagg 9360 ctcataactc ctatccacag cccttgttat ggttttttga tataatgtta gatgtgttag 9420 gcctcagggg caggcctctg accttctgct ctccttccac cctaatcttt ccccacctga 9480 ttgtgggcct taaaaccctc ccctgagagg ggtggaccct gtatcctggg ggaaggaatg 9540 ctgatgtcat gaagcttcca taaaaatcca agaggacaga gttcagggag cttgtggata 9600 gctgaacaca cggaggttcc tagagagagg cctgctcagg gagggcacag aaactacacg 9660 cctcttcccc catacctcgc cctaagcgtc tccttatctg tatcctttgc gatatccctc 9720 ataataacct ggtaaacgta agtaagtgtt tccctgggct aggtaggctg ctccatcaaa 9780 ttaattgaac tcaaagaggg ggtcatggga accccagcct gaagccagtc ggtgagaaat 9840 tctggaggcc cagacttgca actggtatgt ttgaggggtg gtggtggaga ggcggggcag 9900 tcctggggac tgagccctca acctgtggga tctgacactc tctccaggta gacagcatgg 9960 gaactgaatg agaggatacc cagctgctgt ccgctggttg gtgtgtgggg aagaaacccc 10020 ctcacatttg gtcacagaag tctcttgtgt tgatgattat ggtgtgagag tagagaaaaa 10080 agataatttg agacagtttc tctctccagt aggtgtcttg tttgttgtac acatgaagaa 10140 accaagg ttc tgtgatgaaa taacttgctt aagtttaagc agcccagaaa taatgtagtc 10200 acaatttgaa cccagctctg cctgattaca aaacctgtca taggtagtgc tatatcatgc 10260 cccatccttg atcccttttt tttttttttt tttttttttt ttaaagagag agacttcgct 10320 ctgtcaccca ggctggagta cagtggcgag atcttggctc actgcaaatt ctacctccca 10380 ggttcaagtg attctcctgc cttggcctct ggagtagctg ggactacagg catgcgccac 10440 cacacccagc taattttttg tatttttcat agagacgggg tttcaccatg ttggctagac 10500 tggtcttgaa ctcctgacct caggtgagct gcctgcctcg gcctcccaaa gtgctgggat 10560 tacaggcatg agccaccgtg cctgacccct gatccctttt ctattccttt tggatggctt 10620 tcctcttgtg cctgccacat cccctctttc tcgaacagtc tgatctgcca ggccatggca 10680 agcctcattt cagcgcactc tcctttgctg ctttggaaga gtgagagtag cattttcttt 10740 gcagtttcct ttctcagagc tgtactgtct ttaagccctg gacatatggc ttccatgctg 10800 agaaaacatc tctcatagct gtggcgtcat gctgaggagg aatgtagaaa cggaagtggg 10860 gtcacttcct tcccttgtct ccagaggaat cattaacatt gcacaagaac ttgttggttt 10920 gggcacacat gtgggtccct atcccgcttt gggtcggtct gtcttctgcc cactctttgg 10980 gatggttagt tactaaaagt ccgttttggc atttggtctt catgtacctc ttccgggtgt 11040 tctttctgct tttcaggacc ttgtgcaaca tactttggcc ccatttggaa gtttttcacg 11100 tgtgcttgct ggggagcaac acctcatttc caatctagag agagtgagct tactgctggg 11160 tggagaactg agcagtaaga ttagggtggc tggggaggca gtgacagacc caggcacagt 11220 cagctgtgca cacaccccct catccaggag ggcctttgcc ctttgacttc ctgggattgg 11280 tgggctctgc tcaagaagta gctttcgccg tggtgggaac tctgagggac ctggcagggg 11340 tccacttttg gggtcggaat tcccagtctt accctgggca ctccttctgg tgtgtacgcc 11400 tctttttgat cttttttttt ttttttttga gatggagtct tgctctattg ctcaggctgg 11460 agtgcaatgg cgcgatctta gctcactgca acttccagct cccaggttca agcaattctc 11520 gtgcctcagc ctcccgagta gctgggatta caggtgcctg ccaccatgcc tggctaattt 11580 ttgtattttt agcagagaag gggtttcacc atgttggcca ggctggtctc aagtgatcca 11640 cccgcctggg cctcccaaag tgcagggatt ataggtgtga gccaccatgc ccagcctctt 11700 ttgacctttg accaaagacc tctaggactt tggagtcaag tctcatgacc tctaaaatgt 11760 tgttatatga aaagataagc tacaaagata tgtaggattg agtgagagca agagagac ag 11820 tcacacaaaa atagactgtg ataatatggc tgggtacggt ggctcacgcc tgtattccca 11880 gcacttcggg aggccgaagc agatggatca cttgaggtca ggagtttgag accagcctgg 11940 ccaacatggt gaagccctgt ctacgaaaaa taaaaaaatt agctgggtgt ggtggtacat 12000 gcccgtactc caagctactc aggaggctga gtgaggcagg aggatcgctt gaacctggga 12060 ggtggaggtt gtagtgagct gagattgcac cactgcaccc agcctgggtg acaaaagtga 12120 gactctgtct caaaaaaaaa aaaaaaatta taataaacaa aatgttgggc cgggcgtggt 12180 ggctcatgcc tgtaatccca gcactttcgg aggacaaggt gggtggatca tttgaggtca 12240 ggagttcaag acctacctgg ccaacatggt gaaaccccac ctctactgaa aatacaaaaa 12300 ttagccgggc atggtggcga gtgcctgtag tcccagttgc ttgggaggct gaggcaggag 12360 aatagcttga actcgggagg tggtggttgc agtgagccga gatcatgcca cagcactcca 12420 gcctgagcga tagagcaaga ctctgtctca gaaaaaaaaa aaaaaaatgt tggctgggca 12480 tggtggctca cgcctttaat cccagcactt taatcccaac actttgggaa tccaaagtga 12540 gaggatcatt ggagcccaag agtttgggag cagcctgggc aacataagaa gaccctgtct 12600 ctacaaaaaa ttttaaaaaa attagtgggg cgtggtggtg catgcctgtg gtctcatgta 12660 ctcaggaggc tgaggtggga ggattgcttg gacccaagag gttgaggctg cagtgagctg 12720 tgattgcgcc actgcactcc agcccaggta acagcgagat cttgtctcaa aataaaaaat 12780 aaataagtaa ataaaatgtt aacattgctt atcacaaaat gatggggtta tgggtttttt 12840 tccccttcag tttccaaatt ttctgtagca cacctgtgct atactaaaat agaccaatag 12900 tttttgtgtg tgtgtgtgtg tgtgtgtgtt tttctttgtt gagacttggt ctggctctgt 12960 cacccaggct ggagtacagt ggtgcgatct cggctcactg caacctccgc ctcccaggtt 13020 caagcaattc tctgcctcag cctcccaagt agctgggatt ataggcggcc accaccacac 13080 ccggctatat ttttttgtat ttttagtaga gacggggttt catcatgttg gccaggctgg 13140 tcttgaactc ctgaccttgt gatccacttg cctcagcctc ccagagtgct gggattacag 13200 gcataaccca ccatgcctgg ctgttttttt tttttttttt aaatagagac ggggtcctac 13260 attgcctagg cttgtctcga actccaaagt tcaagtgatc tgcctgcctt ggcctctcaa 13320 agtgctggga ttataggtat gagccactga acctggcccc taaccaatag tgtttttatg 13380 tggtcttgct ctgtctccca ggctagagtg cagtggactg atcataggtc actgcagccg 13440 aaactcctgg gctcaagtga ccctcccacc tcagactccc aag tagctag gactgcagat 13500 gcatgccact gtgtcatgct aattttgttt tattttttga gacaaaatct cgccctgtca 13560 cccaggctgg agtacaatgg catgatcttg gttcattgca gcctctcccatct cccaggttg cctcctca 13620 aggtctggtgtc tggctaatta ctgtattttt agtagagacg ggggtttcac catgttggcc aggctggtct 13740 tgaactcctg acctcaggtg atccacctgc ctcaacctcc caaagttctg agattacagg 13800 ggtgagccac cgcgcccagc ctgagacact gttcttatct caccgtcctt tgttctagtc 13860 ctggaggttg cagtgacctg agatccccat attcttgatt ctccccaaag cattccgcca 13920 tctccctagc aggatgtccc gaggtaggtg ttgctgggtt cgctcacgtt agcttctctt 13980 gcagcgcaat gtccctgctc ttctctcgat gcaactctat cgtcacagtc aagaaaaata 14040 agagacacat ggctgaggtg aatgcatccc cacttaagca ctttgtcact gccaagaaga 14100 agatcaatgg catttttgag cagctggggg cctacatcca ggagagcgcc accttccttg 14160 aaggtaaggg ggcaccggct cagccaggcc cgctcttacc tgtttagata tttagctagt 14220 gggatttgcg ggtggggagg tatatctctg ctcccaggga agtcataacc agatagcctt 14280 agaaatgctc tgctcttgaa atcatgtgga attggagtct ggggagatga ggccaggaag 14340 acaagggaac tgccatttag taattacttt aagaagtcca catgttttgt ttcatttaat 14400 catgaaagat aggtgaatct tatccccatc tacaaggcaa gcaaattgag gtttgagagt 14460 tgtccgatac cttgtaagta atagatcagg gttttaccca ggtgtctcca actccggag c 14520 ccacgtgttt tcctttgcct agttctggct gccattaata ccaggcactg tttgggtgtc 14580 agtgatccct gaccagggcg ccctttcttt gatattttta gattggtgac agaactatag 14640 gaaactcacg tcacctatgc cttttctact agttactaat ttgagcccaa cctctagttc 14700 cttgtctatc agcagagaag gactggttcc cagggccccg ctcgtctgag tgtcaactcc 14760 agcactggct tcctcaggct ccctctttag aaggacactt tggcgccagg tcctgagatt 14820 gtctgtggag acccagtaac tgagtcttat ctgcccttgg tttcctcact tgtgaaagtg 14880 gggctcaggt gtgaacagtg gtttcagatt ctagtctgca gaaccctaga agttcagtgg 14940 tggtgcttcg ggggttggcc cctggctcag gggggccacc ttctttccat tctgctttca 15000 aatagagcag cttggcttct cgttgtttta catgccaggc tgctacacga ggctcccttt 15060 gaagaaagag actgtccaac ttcatgccct catctgtgcc ctgccctttg cgtggcgctt 15120 gatgccacag aaggtgtttc ctaaatactt gttgaactaa aacaaatctg aattttaaag 15180 tagtatgatt ggtagttgaa ataatcccaa ctcattgcct gtttatcagt ggctatggtg 15240 tgccagccgt gtgccaggtg ctgggcatgc agagatgggg tagatcaggc cgctcccctc 15300 tgggagccca gactaaccct cttggaagct ctgagtcacc tactcagatg a tgtggtgga 15360 caaacacatg cgtaatgcag gtgtttatgt gatgcatgct ttgatttctc tgtgtgtgaa 15420 aatatgaggc ctcccctgtt gtttaaatgg aagggaaaga gctggagtga tcctgagctg 15480 tgctgggtca gggaaagaat ggttcctgcc tgggcactgt ttctaacggg caggtaacaa 15540 gagcttgttg gcctgctggc tctcgctttt aagtgtagtc acatgcttga gagccagctt 15600 cctccgaggg acattccaaa ggccatgccc aggtgcccat ataagggagg aatattgatg 15660 catctggggc agaggtgacc ggtggacata gcgggagggg agatgggggt gttgttgact 15720 tggtcatttc ctttcatggc gctggctgtg ggggaaattc actgggcagg cccttgtcat 15780 gcttcagatg ctttcggagt gttctcaggg ctggggcctg tcagcctcat agttcccagg 15840 agtgtgcaga ggggctaaac acccgtctgt gttccagcca gctgaagcac ctgtctggtg 15900 cgagttctag acaggacagt ggtgggatct cactcagttg ctcctgtatg gagtaatttg 15960 ttggccatct gcctgttaag ggcactggga aaaagcagtg cagtgatcca aaggtctgct 16020 cacgtagcaa acatttgtgc acctgctggg tgctggggtc agatagtgtg gccctcatga 16080 ggcagatgac acagacaggt gagctgtaca gttaccttat ggtgaggtga gagctgagat 16140 gggagaagtg cagggtgctg gggcacacag agaaggggtc cggg gtcctg actcaggtta 16200 gaggggtggt cagagaaggc ttcctggggg cagtgctgtc taagctagac cggaggccaa 16260 gtgagaatga gccacgctgc cccaaggcag tgtagtgtgt cagttcttca gagagtgggg 16320 agggggtttg ggcagcaggg aatggagcca gagggccgag gaagctggtg agtttgggga 16380 tatggtgagg gagaccgtga ggcagcgggt ggcaagaggc caacagggcc agggcaggga 16440 gggcctcaag aagtgacata gaggccgggc gcggtggctc acgcctgtga tcccagcact 16500 ttgggaggcc gaggcgggcg gatcacgagg tcaggagatc gagaccatcc cggctaaaac 16560 ggtgaaaccc tgtctctact aaaaatacaa aaaattagcc gggcgtagtg gcgggcgcct 16620 gtagtcccag ctacttggga ggctgaggca ggagaatggc gtgaacccgg gaggcggagc 16680 ttgcagtgag cccagatccc gccactgcac tccagcctgg gcgacagagc gagactccgt 16740 ctcaaaaaaa aaaaaaaaaa aaaaaaaaaa aagaagtgac atagagtggt taagagcctg 16800 gactccagag ccaggctgcc taattcaaat catagctctg ccttttacta actccggagc 16860 catgttaata aagaggacca aaggagagaa tgaatttgaa aagtgaaagg aacagtgcct 16920 ggcatgtggt aagaacttcg taaatatttg ttaagaaaaa ataaacaagg aaggcagtta 16980 acatttaagt attcatcttc taccagccgc cattcac agc atttgtgttt caattcgttt 17040 gatcctcacg atgttctcat gaagtagata atgtagttat ctccgttttg tgcttaagga 17100 aactaagacc cagaaaggtt cttttgcttg aggccacaga gctagaaagt ggtaagaacc 17160 agcttcagaa ccaggcagtg ttgctcctga gccatcttct taaccaccac accgtggggc 17220 caccatagag gatctggagc tcagcctgtc ggctgcaggg tgccagaggt ggactcgttt 17280 agggtaagca gggccggcgc tctggccctt ccagacttgg gactgtggaa ctcctctgac 17340 cacgtggtga cccattttca atccccacct ccagacacgt acaggaatgc agaactggac 17400 cccgttacca cagaagaaca ggttctggac gtcaaaggtt acctatccaa agtgagaggc 17460 atcagtgagg tgctggctcg gaggcacatg aaagtggctt tttttggccg gtaagtcctt 17520 gaggcaccca ccctttcttt cttcctggct aggagggagg gaggcacttt gtgcaaggtc 17580 acagaacgtt ccaggcaggg acatgcttca gaattcattg ctttcctctt aatttatatt 17640 ttatttattt atttgatttt tgagacaggg ttctctgtca cccaggctgg aagctcatta 17700 cagcctcgac ctccctgggc tcaggtgatc ctcccacctg agcctcctga gcagctggga 17760 ctgcaggtgc atgccatcaa gcctgacttt ttttttttta ttttctaatt aaggttaaag 17820 gcatataata taaaatttac caccttaacc attttaagca tacaattcac tagtaggtat 17880 atttgcaacc agtctcaaga gctttttcat cttgcagaac tgaagcttgt attatagcca 17940 ttaaacaata actccctgtc ccacatgtcc cttgccctgg ccaccaccat tctactttct 18000 gtccctatga atttgactat tctagatgtc tcacgtaagt ggaatcatac agtatttgtt 18060 tttttttctc cattcataaa acttttattc cacttacatg aatttaatac acgtgttctt 18120 aacaattgtg cttgtattgt tcatgaaaat ttcgtaagac attaaacaaa gctagccatc 18180 atctcaagtt atttccctgt taactatttt tacagtgcat gcatgttagg caagcatcaa 18240 aaaaaaatca caaaagcaaa gaatctaaaa atatgccggg catggtggct cacacctgta 18300 atcccagcac tttgggaggc cgaggcaggt ggatcacgag gtcaggagat cgagaccatc 18360 ctgtctaaca cagtgaaacc ccatctctac taaaaataca aaaaattagc cgggtgaggt 18420 ggcgggcgcc tgtagtccca gctacttggg aggctagggc aggagaatgg tgtgaacccc 18480 agggggcgga gcctgcagtg agccgagatc gggccactgc actccagcct gggcgacagt 18540 gagactccat ctcaaaaaaa aaaaaaaaaa tctaaaaata agttaaatac gtgttttttt 18600 tgttttgttt tactgctagc ttgatataca tgaagtattc atttttcctg catctttact 18660 tttacatttg ttcttaggtt gc ctgaaaca ttttaaatac aaataaaatg agtgtagcaa 18720 aaataatgaa agctaacagc agataacttt acaaataatg gagtgtgaac catttctgcc 18780 cttatccaga ttgaaatggg tcacaacttt gtttaaagga acacttccac agctgtagtc 18840 aaaggtgtgc acattgagat tgagtattcc acagacacac atggtttaac atgtgataac 18900 catggggtat ctgtttctac cacagccttg taagtgctcc aaaccttaaa gtacccacat 18960 ttactatacc tgtgactgga accaatgatc ctttttattc cccaccagga caaaccaata 19020 tgtaggcagt tttctttgct ttgacatgga agcagtttta acactggccc ttgtgaagcc 19080 acaatgtacc taaagtacta ggccaaacat ttataacttg tataatgacc aggcacggtg 19140 gctcacgcct gtaatcccag cactttggga ggccaaggca ggcagatcac aaggtcagga 19200 gattgagacc atcctggcta acacggtgaa accccatctc tactaaaaat acaaaaaaaa 19260 ttagctgggt gtggtggcgg gcgcctgtag tcccagctac ttgggaggct gaggcaggag 19320 aatggcatga acccaggagg tggaggttgc agtgagtcaa gatcgcgcca ctgcactcca 19380 gcctaggtga cggagtgaga ctccgtctca aaaaaaaaaa atctaataca agaaaaaagg 19440 catctagaca gattgtatgc taggcacaca gtggtagcct ggggaatagg cactgtgcct 19500 agagtcttga ccctg atgaa ctgtaacctc aggtgtggtc ctctactcct ctgaactatc 19560 atttcttcat cctcacctgg gaggtatgac cccctactta ggtgagctag tgggcttcct 19620 gtctcactgg gtgcaggtaa ggtcattgta gttctgctct agtgatatgg ctgagaaact 19680 tcaactcaga atgagaaaca ctgtgtttag cacaggttga aagctctgag actcagagca 19740 gatgagtctg ctgagcagag ctattctcaa gaagcagaaa tggtggggtg cagtggttca 19800 cacctgtaat ctcagcactg ggaggctgag gcaggagaat tgcttgagcc caggagtttg 19860 agaccagcct gagcaacatg gcaagacccc atctctacca aaaatttaaa aattagctgg 19920 gcgtggtcgc agctatagtc tatatatagg tggtacctat agtctaagct acttgggagg 19980 ctgaggtggg aggatcattg agccctgatg gtcgaggctg cagtgagcta tattcatgcc 20040 acacgctact gcactccagc ttgggcgaga aagcaaaatc ccatatcaaa aaaaacaaac 20100 aacaacaaca acaacaaaaa accccaccag aatacatgaa aggggtgtga taggtaagaa 20160 tgagaaagta ggctgggcat ggtggctcat gcctgtaatc ccagcacttt gggaggttga 20220 agcaggcaaa tcacctgagg tcaggagttc gcgaccagtc tggccaacat ggtgaaaccc 20280 tgtctctact caaaatacaa aaattagccg ggcatggtgg cacatgcctg tagtcccagc 20340 tacttggg ag gctgaggcag gagaattgct tgaactcagg aggcagaggt tgcgatgagc 20400 tgagatcgtg ccactgcact ccagcctggg cgacagagtg agactctgtc tcaaataaca 20460 ataaaaaaaa aaagaatgag aaagtaacct tccctttagc ctggcagttg tcagctggga 20520 ctcacaaggt ggagcttcat gtgtaaggag gagcgtgatg ggagaaaagc agcgaaacat 20580 gccctgtgta ggaaacactg gggtcattta gactgaggaa gacactctgg gggttagtcg 20640 gtgtttcagg taggcctgaa ttaaactttg gagacagaca gacagtctct ataagtgttg 20700 ctgagggtgg ctgtgtgatt caaggggcag acagcacgca gtgagcctct tgtagattga 20760 tctgaaatga tgaaccaggg gcatgtcaga ggtttgggcc tgggggtgtt tattcatttt 20820 aataaacagt gcctactctt tgtctctcag cactgtctgg gcactggcaa cattgcactg 20880 aatagggctt tggccttcca ggctggtatc tgcgttgtga aagtaattca ggtcagatac 20940 tggtggcttt gctgacagct gttacttcct tctaggacga gcaatgggaa gagcaccgtg 21000 atcaatgcca tgctctggga caaagttctg ccctctggga ttggccacac caccaattgc 21060 ttcctgcggg tagagggcac agatggccat gaggcctttc tccttaccga gggctcagag 21120 gaaaagagga gtgccaaggt gagggtgcca ggctggctgt cagccctgtg cctgctgggg 21180 gagcaagtcc tccgttgtga cacggctgac ctcacctcta gggagggggc agcaccaccc 21240 tgtggaggtg aatgggagac cctattttag atgaactttt tgaaggatgt aagagctttt 21300 gggggctggc ggaggtggtt ttcagaaacg tggatcacca agaatgagga ccctgctgtt 21360 cctcatacag ctctgagtgg ccactgacct ctcttcacta attaaggaag acagtatgtg 21420 ctgtgttctt ccttgggata ttgccagaga ggaattcctg catttgctgt gtgtctctga 21480 cctatcagtc agggaccgac actctgagtt ttctgagagg cagctgcatt cttgtttggg 21540 gccagatgct caatttgctt cataaagaat caattggtcg ggcacggtgg ctcacgcctg 21600 taatcccagc actttgggag gccgaggtgg atggatcatt tgaggtcagg agttcaagac 21660 cagcctggcc aacatggtga aaccccatct ctactaaaaa tacaaaaatt agctgggcgt 21720 ggtggtaggc acctgtaatc ccagctagtc gcgaggctga ggcaggagga ctgcttgaac 21780 ccaggaggtg gaggttgtgg tgagctgaga tcacgccact gtactccaaa cctgggtgac 21840 agagcgagac tccgtctcaa aaaaaaaaaa aaaaaaagaa tcaatcagtg cagaacagat 21900 gactttaggg tgatatccgg gaaagaagct caaaagagca gtgaagaaag tgggttctta 21960 ttgacaactc ccagctgtta actttttata tgtggaagaa gaaagggtag cagatgggc a 22020 gcattcccag ccactgtgct gtgatgcagc ggcacaggaa atctcctggc ctggtggttc 22080 ctcctcagcc tctttctttc cttcctctgc catcagactg tgaaccagct ggcccatgcc 22140 ctccaccagg acaagcagct ccatgccggc agcctagtga gtgtgatgtg gcccaactct 22200 aagtgcccac ttctgaagga tgacctcgtt ttgatggaca ggtaagaggg aggtgccctc 22260 ctaggaggtc caaactggaa ggcaccaggt ttccatttct ggataatcta ggccagggtc 22320 cctgggcccc atccttgctc tgcttaagta ttactacctt tcagatgggc tcaaccaaat 22380 cctctggcct cttggctttc ctctggagtc tggttactat acctatttat cagtaccctg 22440 cagatgtccc agggatggtc tgtaaaagat aggagggatg ctcaaaagtt ggtgggggag 22500 gttctcaatc tcagcccagt gccagcatgt gtactggggg tggggagttg tggcggagtc 22560 agaggtgtat tttatttccc ttgaagaaac actttgcctt ggcttggtgg actggttata 22620 actataccaa catgtacagt attgtagtgt ttatctggac tcacatcaag tcacaacttc 22680 accttaattg tatatcatct tttttttttt tttttttttt tgagacagag tcttgctctg 22740 tcacccaggc tggagtgcag tggtgcagtc tcggctcact gcaacctccg cctcccgggt 22800 tcaagtgatt ctcctgcctc agcctctgga gtagctggga ttacaggtgc c cgccaccat 22860 gcctggctaa attttgtatt tttagtagag acggggtttc accatgttgg ccaggctggt 22920 cttgaactgc tgacctcatg atccgcccac cttagcctcc caaagtgctg ggattacggg 22980 catgagccac tgtgccccgc ctggtatatc atctttaaag tagaattaca agtaattctg 23040 tttttataaa agcaatagat atatgtgttt aaaaacactc atttctgcca ggcacggtgg 23100 ctcatgcctg taatcctagc actttgggaa gctgaggcgg gcagatcagg aggtcaagag 23160 atcgagacca gcctgaccaa catggtgaaa ccctgtctct actaaaaata caaaaaaatt 23220 agctgggcat ggtggtgcgt gcctgtagtg ccagctactt gggaggctga ggcaggagga 23280 tcgcttgaac ctgggaggcg gaggttgcag tgagccgaga tcgtgccatt gcactccagc 23340 ccgggcgaca gagcaagact ctatatccaa aaacaaaaca aaacaaaaca aaacactcat 23400 ttccaggcaa tctggaagga taggaaatgg gaagtaaaat ccgttaatga gggccaggcc 23460 tgatttctct cccgtccctg gctttctcct ccatgacctg cctgcctcac aagtcccagg 23520 tctgttctca gcaggaagaa tagggctcct gctctgcctg atgatttggt ttacccctgt 23580 cacctttagc cctggtattg atgtcaccac agagctggac agctggattg acaagttttg 23640 tctggatgct gatgtgtttg tgctggtggc caactcagag tcca ccctga tgcagacggt 23700 aactcctcct ctgccttctc ccaagctccc agcaccccct gggcaggcag ctgatggggc 23760 cttggctgtc aagctcctgc tccaccgagg tcttaccctt tatctaggaa aagcacttct 23820 tccacaaggt gagtgagcgt ctctcccggc caaacatctt catcctgaac aaccgctggg 23880 atgcatctgc ctcagagccc gagtacatgg aggaggttcg tgcttctgtt tggcagtttg 23940 gggaatgcaa ccccgaggga gcactgcctg ccactggggc cacttcctgc acccctggat 24000 ctagtgactt ccctgaatta attttattgc caaagaattc tccaaatact cttctgtggt 24060 atcttctggt ggtctttccc ctctcccatc cactttgctg gatgcacccc ctcttcaagc 24120 cactctcccc taggcctaaa aaggatccct gctgcattag tgcatcttca gtgccggtgc 24180 tcagtttggt ttctgaggaa ttaatacaga acaggctcag ctctagggag gcctctggga 24240 aagaggctgg aaagaggaat ggagaataac tggttcttac tggtgactta caactcttag 24300 ctatttactt atttatttag agactgggtt atgaggctgg ctaatttttg tatttttggt 24360 agagacgggg ttttgtcatg ttgcccacgc tggttttgaa ctcctgggct caggtgatcc 24420 acctgcctca gcctcccaaa gtgctgggat tacaggcatg agccaccacg cctggcctca 24480 caactcttta aatatgtgaa atgaaagaca aaagggt aac aaacggcatg gtggtgcaca 24540 cctataggct gagagaggat cccttgagag taggaatttg aaaccacctt gggcgacgta 24600 gcaaaacgcc atctccaaaa aaataaaggg taggccgggc gcggtggctc acgcctgtaa 24660 tcccagcact ttgggaggcc gatgtgggcg gatcacgagg tcaggagatc gagaccatcc 24720 tggctaacgt ggtgaaaccc cgtgtctact aaaaatacaa aaaattagcc gggtgtgatg 24780 gtggcaggcg cctgtagtcc cagctacgcg ggaggctaag gcaggagaac ggcgtgaacc 24840 cgggaggtgg agcttgcagt gagctgagat tgtgccactg tactccagcc tgggcaagag 24900 agtgagattc cgtctcacaa aaaataatta aacaaataaa gggtaataag attgataata 24960 tggcaatatg ggatgataaa acatgcagtt gcagtcagta ggccagagtc caaattattt 25020 atttttttcg agatggagtt ttgctcttgt cacccaggct ggagtgcagt ggcacaatct 25080 cggctcactg caacctccac ctccagggtt caagcaattc tgcctcagcc tcccgagtag 25140 ctgggattac aggcacccgc caccacacct ggctaatttt tgtattttta atagagatgg 25200 ggtttcacca tgttggccag gctggtcttg aactcctgac cttaagtgat ccgcccgcct 25260 cggcctccca aagtgctggg attacgggcg tgagccactg tgcccgcttg tgactgacac 25320 taggcagtgt gactttgtta gcttccttga cccttctggt aatccaattt cctggccagt 25380 gtgatggtgt gataaataga cttgttgaga ggatgaaatc tgtcaaaaac acctgacacg 25440 tggtaggtgt ctacaagaag ctattgtcac attttatcgg tagaacccca ggatgtcaga 25500 gccaggaggg atgttctagt gatgcccact ccttttataa acaatgcccc aggaggacac 25560 agcttgtcag agtataggtc ccagatttag gtttcttgac ctcagggttc ctttcctgcc 25620 agggcaccaa aatgaggcac tatataggct taacaagagg cagtctaggg cacgtttaag 25680 catttctgct tgtatatgca taaaatatct ttggcacgct cactgccaag taacccccac 25740 acgttggtct gggaaacttg gtggtgagag agcagaatag tgggggacat tacactgtgt 25800 atgtgtttgt gcctttggct gttgagccat gtgaatatat tgcctattct tttttttccc 25860 tcactctgtc gcccaggctg gagtgcagtg gtgctatctt ggctcactgc aacctccgcc 25920 tccaggttca agtgattctc ctgcctcagc ctcctgagta gctgggatta caggcgcctg 25980 ccaccatgcc tggctaattt ttttgtattt ttagtagaga cagggtttca ccatggtggc 26040 caggctggtc ttgaactccc gacctcaagt gatccacctg ccttggcttc ccaaagtgct 26100 gggattacag gcgtcagcca ccatgcccag cctcttatga cctattcttt taataaaaga 26160 ggcaggtggg ggctgtgggg cc acctacac tcactctgga cacatttgtt tgggctccag 26220 gtgcggcggc agcacatgga gcgttgtacc agcttcctgg tggatgagct gggcgtggtg 26280 gatcgatccc aggccgggga ccgcatcttc tttgtgtctg ctaaggaggt gctcaacgcc 26340 aggattcaga aagcccaggg catgcctgaa ggaggtaatg atgagaacag atgtcctcct 26400 tttctctgat gtttgagaca ttttgtctcg tgctgaggag tctgtcagta gaaaatcctt 26460 ctgagaaggg gatgctgaga agagcagaga ggctcttgct ctttagccag tggcttggtt 26520 tctggggatt tcatcgtttt cctctgctgc caagttgttt ctggactaat gcagtacaat 26580 cctcctaggg ggcgctctcg cagaaggctt tcaagtgagg atgtttgagt ttcagaattt 26640 tgagaggaga tttgaggtga gtcctctgat tctggtatct ggcgattctg tgcctcccca 26700 gctcccattg gctgtgtccc tggcagtgaa aaccagagtc tggcccttgg ttgtaggccc 26760 ctggtggccc ccacctccct ccgtgcctct gtgtgttcca ggagtgcatc tcccagtctg 26820 cagtgaagac caagtttgag cagcacacgg tccgggccaa gcagattgca gaggcggttc 26880 gactcatcat ggactccctg cacatggcgg ctcgggagca gcagtaagag tccaagactg 26940 cagataggtg gagagagctg ccgagacaag ggtgctccac ccctgccttg ggcagttagg 27000 acacgccttg atggc agggc tgagtctctg ggctcccatc cagagccctt tccctggcca 27060 ccagaccatg ttagaaccac atagacagcc tggtgaggag ggcggctggg tgcttcatca 27120 ccccacctgg tctgtgcagc acgattccct gtgttggagg tcttggccca tgccctgctg 27180 agctctgcag ccattctcag agcctgaaca catttttcaa aacaacattc cagccaggag 27240 cagtgactca cacctgtgat ctcaacactt tgggagacca aggcaggagg actgcttgag 27300 gccaggagtc cagcccagat aacatagtga gatcccatct ctacaagaat tttaaaaatc 27360 agctgggtgt ggtggcacac acctgtagtt ccagctactt gggaggctga ggcaggagga 27420 tcactggagc ccaggagttc agggttccag tgagctgtga tcgcaccact gcactccagc 27480 ctgggcaaca gagtgagacc ctgtcactaa agaataatat gaaaacacct tgcttctatc 27540 acacagaatt gactttctca gttgtttatg ttaagttaaa attttaaaaa gttcagttcc 27600 cttttgattc ttttgcactt gtcccagtgt cactctgccc tgaggcacca taatcatgaa 27660 tttacattgt tctatcaccc aaacagtact ttttacataa atgctgagga atatgtcagg 27720 tttttacatg cttcagatgt acagatgtgg catcacactg tctgtcactg tacctggctt 27780 tgctttctca acatcgtttt tccctctatc cttgttagta tgtgtagttt gtttatgaat 27840 cggaactgct gtagtatatt ccattgtgaa tatgcctcat tttcctatgg atggaatttt 27900 agattttttt tttgctattg gaaaccctac tgcagtggac atttgttcat gtgtcccctt 27960 gtgcaccttt ttaagagtct tcctggggtg agttcctagg ggtgtggcaa ttctgtttta 28020 gggtacacgc gtttccagtt ttagtcagtt ttcttgatgc tcttcagaac agccgtacc c 28080 attcattgca tgaccagtac tgtccatagg ccttccattt catcagccct caccagaact 28140 tcagacctgg tcatttttgc caggccttgg tagaaaatag tgcctttaaa aatttcattt 28200 acaactgggc atggtggctc acgcctgtaa tcccaacact ttgggaggcc aaggcggatg 28260 gatcacctga ggtcaggagt tcaagaccag cctgaccaac acggagaaac cctgtctctg 28320 ctaaaaatac aaaattagct gggtgtggtg gcgcatgcct gtaatcccag ctactcggga 28380 ggctgaagca ggagaatcgc ttgaacccag gaagtggagg ttgcagtgag tggagatcgt 28440 gccattgcac tccagcctgg gcaacaagag tgaaactcca tctcaaaaaa aaaaaaaaaa 28500 tcatttctgt gattactagt gaggttgagc ttttttccct atattaattg gcagaatttg 28560 tttaaacccc cttaaagcgc cctccctgtt ttgtgcccac cacctgaccc acatcgaaga 28620 ctgaagagtg catggttggc tgcagggtcc tcttcttacc tgggaagggg aaggccatgc 28680 ccctgggtgc gtgtgtgcag ccctgccagg caagatagcg ggcagggcgg cgtgggattt 28740 ctggcatccc ctcttgctcc tctgcttagt cagacaggaa catggatttc tcaccagtac 28800 tctgctttca gggtttactg cgaggaaatg cgtgaagagc ggcaagaccg actgaaattt 28860 attgacaaac agctggagct cttggctcaa gactataagc tgcgaattaa g cagattacg 28920 gaggaagtgg agaggcaggt gagaaatgag gaggaggcat tctgggaaga tttggactcc 28980 gagtagagtc cagaagaaag cagacctcct cctcttaggg acttctcagc ctttcagaag 29040 aaagttgtgg ccctgtttca agaatacaga gctgccgttt gggttccatt gtcgggttgt 29100 gtgatgctgg gcatgttccc tttcctctgg acctccacag atcatgtggg cgtttgatca 29160 gccagtttcc cagaccctct cacttcagag gctttaattc catagaggag ctgaggaggc 29220 tgctggtttg agaggaagga tgtgccatct gctaggatct ctcctggtgc tgcaggagtg 29280 aactttggtc ttccttgata cttaacagtg tgcttccttt tgctgtaggt gtcgactgca 29340 atggccgagg agatcaggcg cctctctgta ctggtggacg attaccagat ggacttccac 29400 ccttctccag tagtcctcaa ggtttataag aatgtgagtc atggagcaac aggtcctctt 29460 ggcaggaggc ccccaaaagt gattcaaccc ctgttggtct gggtcagggc cccccagcag 29520 tgacagtaga agccacagag acaaggccca ggcttccgtc agccttctgg ggtcacctgg 29580 tggatgtggc ctgaagggaa ttttgatgga catgcttctc ttaacttccc tcttctggtg 29640 gcaggagctg caccgccaca tagaggaagg actgggtcga aacatgtctg accgctgctc 29700 cacggccatc accaactccc tgcagaccat gcagcaggac atga taggtt agtgcccatg 29760 gggaactggg cagctgtggc cctgggctgc ccaaagatca gatgcggagg ccaagctaaa 29820 gaaatcagga ctttccttat ctgtcacttt tcatgatgat tcaactcgat accttcaggt 29880 tctgggtaca tgttctgaac tcattcttgt ttgctttttg agacagagtc gcactccgta 29940 acccaggctg gagtgcagtg gcatgatctt ggctcactgc aacctctgcc accgaggttc 30000 aagcgattct tctgctccag cgtcccgagt agctgcgact acaggcgtga ccaccacacc 30060 tggctaattt ttttgtattt ttagtagaga tggggttttg ccacgttggc caggctggtc 30120 tctaactcct gacctcaagt gatctatctg ccttggcctt ccaaagtgct aggattacag 30180 gcatgagcca ccggccctgg ccttgtgaac tcattctgta cacagggttg gggagcccat 30240 cttgttctac tacatgggtc taccctaggc tgagaattgg gcctgctcct ttaagtccac 30300 gtgaggcata agtgttagca gtatggcatc tgagtccaca cttgcctggg agagctggcc 30360 tcaagtgagg catgctcagt ctcacgggcc aacttgactg gggtgtggtt cccaggcaaa 30420 gctgttcagc atccctggca gtagctggta gagccctgtc tccaaggctt ggtgccgctg 30480 tggtgcaggg ctgagctgat aagcttttcc tccatttctc ttcctgacag atggcttgaa 30540 acccctcctt cctgtgtctg tgcggagtca gatagac atg ctggtcccac gccagtgctt 30600 ctccctcaac tatgacctaa actgtgacaa gctgtgtgct gacttccagg aagacattga 30660 gttccatttc tctctcggat ggaccatgct ggtgaatagg ttcctgggcc ccaagaacag 30720 ccgtcgggcc ttgatgggct acaatgacca ggcaagcaaa gttcctcacc tcaagggcat 30780 tgtggggggt cagtctgtac cctgcctcca gacacgggaa ccattctaaa acgggggttc 30840 cctaaggtca gcagctgtgg tggtgtgccc caccacacag tacaccacag acagaatttt 30900 tgctactgat tgacctggat ttgttctaag cagtgatatt tgtgaaatga cagatgctag 30960 tatttttctc taatacacat taaagtaagt atgtaactat taaaaataga cgactttaaa 31020 aataaccacc tacatacgac tattttaaag ttcagatttt aaaaacattc atctctaaac 31080 cacttgagtt gtcacatgta ctgccctctg tacacatccc acactcgggg aagctgccag 31140 gcgggtcagg atggctcctg ctcacatttt gcacgtcccg tggtgctcac ctgagggtct 31200 cctctgctac atctgaagct agttgtcccc tttttgcctt ttggaaattg aagagccact 31260 ctgtgtccct gttccccaga ctagggcaac actgagggtt cacgtgaatg agagactcaa 31320 tacgtccccc tcacccctct catgtttctc tcctcaggtc cagcgtccca tccctctgac 31380 gccagccaac cccagcatgc ccccactgcc acagggctcg ctcacccagg aggagttcat 31440 ggtttccatg gttaccggcc tggcctcctt gacatccagg acctccatgg gcattcttgt 31500 tgttggagga gtggtcagtg accagttctg ctcgggaagg tgggggcgga gggcaggtgg 31560 gcggggcctg agggctaggt tcctgctgtg gcccctgcat tgggccacac tccacagtcc 31620 actgcatact ttctcctctg tgattgcatg gggagcgctt actccctcac caagtttccc 31680 tcacttcctg ctggacatgg tcccagacct tctggcctcg gtgggatcaa aggagacatt 31740 gaaagaggaa ctcagagtag aaacatgaag gctccttggc tggggccctt cagatggccc 31800 tggtagtgat gggccccaga ggagggtggg ccacagagag gctgcactcc aggctgacca 31860 tgtgctctgc aggtgtggaa ggcagtgggc tggcggctca ttgccctctc ctttgggctc 31920 tatggcctcc tctacgtcta tgagcgtctg acctggacca ccaaggccaa ggagagggcc 31980 ttcaagcgcc agtttgtgga gcatgccagc gagaagctgc agcttgtcat cagctacact 32040 ggctccaact gcagccacca agtccagcag tgagtggccc tgtcggaccc cagcagggga 32100 cttcctttag gcagggtcag ccccatctcc cttccccatc tctcttccca cgtggcctgg 32160 aagccactgg gtcctaagca tcgtagaccc tgggccttca ggtgtgcagg gccagctttg 32220 aggccagtca tggggagagg gg tctcccag ggacactttg cgtcttgggc tgggacgggg 32280 taggcctctg ggccccttgg tgaggaagaa atgggaaggc aagagatatt cctagtagga 32340 gttttagagg ttgccttgaa gagcttgggc tgtggatgta gttttgaagt ttttgaaccc 32400 agcctgatct tcgtgcacat cttaggtcag gtgggaatct attgcctaat ctggggtggt 32460 ggggatgaat atcctcgctt cctttttttt attgtgtgtg gcagaccctt tgctctagac 32520 attttgcacc taattatttc tctgactctt ggaggtagat gttgtcttat agccatctcc 32580 tttttctttc tttctttttt ttttaattga tcattcttgg gtgttttggc agggtcatag 32640 gacaatagtg gagggaaggt cagcagataa acaagtgaac aaaggtctct ggttttccta 32700 ggcagaggac cctggggcct tccgcagtgt ttgtgtccct gggtacttga gattagggag 32760 tggtgatgac tcttgacaag catgctgcct tcaagcatct gtttaacaaa gcacaccttg 32820 caccgccctt aatccattta accctgagtg gacacagcac atgtttcaga gagcaccagg 32880 ttgggggtaa ggtcatagat caacagcatc ccaaggcaga agaatttttc ttagtacaga 32940 acaaaatgga gtctcctatg tctacttctt tctacacaga cacagcaaca atctgatttc 33000 tctatctttt ccccacattt cccccttttc tattcgacaa aaccgccatc gtcaccatgg 33060 cccgttctca atgag ctgtt gggtacacct cccagacggg gtggtggccg ggcagagggg 33120 ctcctcactt cccagaaggg gtggttgggc agaggcgccc cccacctccc ggatggggcg 33180 gcggccgggc ggaggcgccc ccacctccct cccggacggg gtggctggcc gggcgggggc 33240 tgaccccccc cacctccctc ccggacgggg cggctggccg ggcggggact gaccccccac 33300 ctcccggacg gggcggctgc taggcggaga cgcttacttc ccagacgggg gcggctgcct 33360 ggcggagggg ctcctcactt ctcagacggg gcggctgccg ggcagagacg ctcctcacct 33420 cctagacggg gttgtggctg ggcagaggcg ctcctcacat cccagacggg gtggtggggc 33480 agaggcgctc cccacttcct agatgggatg gcggccggga agaggtgctc ctcacttccc 33540 agagtgggca gccgggcaga ggggctcctc acatcccaga cgatgggcgg ccaggcagag 33600 acgctcctca cttctcagac ggggtggcgg ccgggcagag gctgcaatct cggcactttg 33660 ggaggccaag gcaggcggct gggaggtgga ggttgtagcg agctgagatc atgccactgc 33720 ctgggcaaca ttgagcactg agtgaacgag actccatctg caatcccggc acctcgggag 33780 gccgaggctg gcagatcact gccggttagg agctggagac cagcgcggcc aacacagcga 33840 aaccccatct ccaccaaaaa aatacgaaaa ccagtcaggc gtggcggcgc gcgcctgcag 33900 tcgcaggc ac tgggcaggtt gaggcaggag aatcaggcag ggaggttgca gtgagcagag 33960 atggcggcag tacagtccag cttcggctcg gcatcagagg gagaccgtgg aaagagaggg 34020 agagggagac cgtggggaga gggagaggga gcgggagagg gagagggcca tctccttttt 34080 caaaatcaag aaactgagat ttgaaaaata gtgacgtgtc ctctgctact cggctgatga 34140 gcagcagagt caggatctct tgtgcccaaa ccagggtgca cagctctgtg ctgttgcttc 34200 tcaggccgtg gtgatgctga gtgtgtttgg ttgttatgat tattcagtct cagcagggtg 34260 taggagattc tgccaaacca ggcctggctc agggcagagc tgctggcagg gatatagaca 34320 ggcccagctg ctccctactg tgggtgggct aagccaccag tggcatcttg ctccacacac 34380 cccaactggg tcccttctct ctcctcccca gggaactgtc tgggaccttt gctcatctgt 34440 gtcagcaagt tgacgtcacc cgggagaacc tggagcagga aattgccgcc atgaacaaga 34500 aaattgaggt tcttgactca cttcagagca aagcaaagct gctcaggtga ggctggcccg 34560 tgtggccaaa ggttagggct ccagggtgca gcccaggcac actggggctc agctgctggg 34620 cttgcgtctt gggtgtggac acaaattttt ctggtgggga tttagctcat tcgtgttaga 34680 tgtgtaccat gggctgggca cggtggctga cgcctataat cccagcactt tgggaggctg 34740 aggcaggtag atcacctgag gtcaggagtt tgagactagc ctgaccaaca tggtgaaacc 34800 ccgtctctac taaaaataca aagattagct ggtcgtggtg gtgtgtgctt gtaatcccag 34860 ccactcgaca ggctgaggca ggagaatggc ttgaacccag aaggcggagg ttgcagtgag 34920 ccgagattat gccattgcac tccagcctgg gcgacaagag caaaactctg tctcaaaaag 34980 aaaaaaaaaa tggatgtgta ccacgtacct catggatgcc agatggtgct ggaggctcaa 35040 caagacacag cgtcgctgcc ttcaaggcac ttgcagttga aggcatgatg gcatcacagt 35100 actgcgagac tgcagttcac agcgccctgt gctgaaccgc agggcctaca gcgcttaaca 35160 tgtgctattt cactcagtct tccctatttc tccattttct acatagaaat gttggggaac 35220 ttgtctggca tctcaaaaaa tggccaggct aggaggggag cccaggtgta cttggctcca 35280 gcagcccccg cactctgatt ctccttacct gccactcttt ggacccatga agagggcata 35340 gccccccatt tacccacaga gaagctggtt acttggctgg ggactggcag agctgggatt 35400 ggaactcaga tctccatggg gtctctgtgg agctttcatt aataatttgc tggaaagaac 35460 atcagtctga ggagtctgga gtcctgggtt ctctccagcc actcatgttt ggcccaagct 35520 cttaactctc ctgtgtctca ttattttcat ctgtaaaatg ggagtaagat ttcatgaca c 35580 agctgcatga ctgaatgtaa aagtgccctg caaaagtcct cggcctgccc agctcatgat 35640 aacatcagca cacggggacc ctgcagaagt cctcggcctg cctagcccat gatagcatca 35700 gcacacgggg accctgcact tcctgccctc gcccctgacg tcagagtctg cagggtggca 35760 gagccagagg ggagatggat cagagggtgt cagctccaac cctcaccgga tcactgggct 35820 ttccctgccc tcgactgcgg atgtgccccc cccgcacccc cacctgggga cagctgcttc 35880 ctagggacac actgcttgcc ctctggggac tctcagaaag ggggaatggg gggaactgct 35940 gcttgtcagc cacagtgaca cagctgaacc tggaaagggt ctcctgtcag cacctcaacc 36000 ccttgcttgg caagcaggga aactgagttc ccgacagctc agggacttgc acatggtcct 36060 gggtgaagga aacacagggc ttgagaatca gaaaagccta acttgtagct ccctggctta 36120 ttagccgtgt ggccttggga agttatttaa cccctctggg cctccttttc cccatctgtg 36180 agacagggat aaacacgatt gttggaggat gatgtaaggg tgtgtgtcaa gcgtccttag 36240 gatggtgcct ggcgggtagt cctaatactg cctatcatca gctatcatgg ttacaaaaga 36300 accatttctt tgcaggaata aagccggttg gttggacagt gagctcaaca tgttcacaca 36360 ccagtacctg cagcccagca gatagtgggc acctgaggcg gagtctgcgt g gagaggggc 36420 ggtgctgcca gccctaagtg ccatgtgggc tcccccaggg gcacgtgtgg ctcctgcccc 36480 ctggccactg ccaagagaat gaagcaccca gtctcgtacc attttgagcc ctccagcact 36540 acttattttc ccccaccttt gcctgctgtt gctggaagag ctggctcata cccccaaagg 36600 acactttcag cgacagctat ggacagcatg gtaccaagga gttaagttga ggctttttcc 36660 agctttctct ggttcatttg attgcttgat aaggcctcag gatctcagca ttgcacaatg 36720 cctcatggaa gcctttgagg gtatcacaca gacaccccca ccttcctcca gcctgtgcgc 36780 acctgccctc cttgcagccc agcacacctg caggtgtaag ggacgattgg agtttcttcc 36840 cagagagtct gtcccagaag gactgtggct tgtgtgtgtc catctcgcct gttggctcag 36900 tgcttcatcc catttgcaga gcctcagaca cgtcttggtg gtgaggctca gttacccctg 36960 ggcttaggct gaggcgggcc ctgtgctggg ggtggtagaa aggatgctgc tgaggcagct 37020 ggaggagtgg gagtagctca gaggggaggg ctgttggatg tatggggagc tggcagagca 37080 ggtggcagtc actgggacaa ggagggactt gcctctcttc tcattattgt gtcctttgct 37140 ttagtgtcag tcctggactt gtgcaggcct gttttgtgta gatctgtttt ggaagatggc 37200 atggtctagg tggttgaagg atgtagtaga aggatggatg gtgg aaggtg gggacgttgg 37260 tggctggctg aggtgcatgg gccccacaca ggacagctgg agaatgggcc gtccacttgg 37320 cctcgttctg cgaggggctc atgggtctga gagcccccac ccactaggct tgattgcatc 37380 cctgttgtgc cctttaagag acatgtttcc accccacccc caaccttgtc ccaagtgccc 37440 tggactaaat ttcctgtgcc agtgactgca gttggccaag ggacaatgtg gaaaacccag 37500 tgtccatctt tccaccctcc ctgatctcca gaaccttcga ctgaccccct tgtctttatg 37560 ctgatgttga gttttgggat tgttactggt tgaagtgggg gcagatgcct gtcaccaagg 37620 tgttgactgt gtgagaaaag cagtttgggt gacaaatcct gtgtggcaca agttggatcg 37680 cttcctagaa ataagcaaca cctctcccaa aaagcagccc acaaggcagg ggcccagcag 37740 cccagccatc actcatcttt gaggaaatga gttggtagcc tctgtgcact gtttggtggc 37800 cacatcacag gtgatgtcct gttcacatac ctgcttgtat ttaaagccct cagtctgtcc 37860 tgttgtgtgg ggcgaagtga tggactctgc caggtggaca tgctgtgggt ggatgttccc 37920 ggcgtgtgcc gggcctgaat ggacaggggc cacttcacag catgtcaggg aaaatcactg 37980 tcacacaatt ccaatggatt ttgtgctctt tttgaaaaaa aaaaattctt tagcgtaaac 38040 atgaattttt tttcaatgta gcccctgggg aatgaat gaa attttgagct tcttcaatac 38100 gtaaaattaa atttatacca ctgagggaga gaccctttct gaaagaagta tggccaaaag 38160 cactttaatg ctgctgacat tgttgttttt atgttcattt gctggagcgc aagacgtgct 38220 gacacagtga gttttctctg atgtatttaa ggtgatgtat ttgcttgagt tactcctgta 38280 tcattgctca taatattgga aactaaaata aaacctagtt ggaaatcctt tgtgagatct 38340 gtctttttgg gttcttgtac aaactcctct ttgacatggc cagctgtatt ctgaagtgac 38400 cataactttg gggaagttcc cactataatc tggctcctta ttcttggaag gaatttgata 38460 ctctcaaaag aacagatggt ttaactgatc tttaaggatc aggaaatcga gactgatttc 38520 tttacagatt tctctgtaac tccagaccat ttttcactta aaaagtaaat gactcactgt 38580 tccatcctgc tttgccttct gagaatttgg ttgccactat tctttttttt ttttttcttt 38640 tttgagatgg agtcacgctc tgtcgcccag gctggagtgc agtggcacga tctcggctcg 38700 ctgcaagctc cgcctcccgg gttcaagcag ttctctgcct cagcctccca agcagctggg 38760 attacaggcg ccgaccacca ggcctggcta attttggtat tttagtagag acagggtttc 38820 atcatcttgg ccatactggt ctcaaactcc tgaccttgtg acccacctgc ctcggtctcc 38880 caaagtgctg gcattacagg tgtgagccac cgcttttttt tctttttttt tttaagggtc 38940 ctcaggagta gatacagggg tagtcagtca gtttccccgc ccctacagct gtcctcttgg 39000 ctctggtgct gcccagtgtc cgccctgctc gcccacagag gccacagctc agctgatagc 39060 aacctcatcc ttccagattc ccaggctaca cacttggggc atcaccctcg agtccacggt 39120 cacacgtgct gcaccaaatc tgtcacctgc tgtcttccaa gtctgttgac cctgcccagt 39180 ttcctcacct ccatcataac catcagcccg gatctaggtt cctgttcctg ccttgtcccc 39240 gcctatcctc gccatcttgt ggctgtttca aggcagcagc caaagacttt ctcaggtaac 39300 aaaaaaaaaa tgaatcatta cctttttttt ttttgcaatc cacaaaacaa gtagtttctg 39360 tcattaacaa gatgttatgt acaacccact aaattgttca cgacagcatc atcccgggtg 39420 atgagccata gtttgaaaaa gggccttctt gccttatgca aatgctgctt agttattccc 39480 agtcaaggcc agcctcattc ctgtgctttc tctgactacc tcatctgtcc actggcgaat 39540 ctgagaggct ggaagtttta gccctctggt aacaatgtcc ctatttaatc acaaaaagta 39600 ggtagtcagg aatgttccac tgtgtcccaa gcactggggt gacagcccag gcagctctct 39660 cggagagcat ctggcttcat aatgtggcct cagaagcagg gccctcctcg cagatgtcag 39720 ctaagttgtt caccgtgtcc tc aagtggat ggtttcctgt gtctaagtgg gactcttagg 39780 cttgagataa gaacagcgaa ctttgagagt ttagtatgtc agacatttca tacgcatgag 39840 ccctaatctc aaaatccaat ggtctatttt ccctaatttc ctcaaatgag gaagttcatt 39900 aattcagcaa aatatttgtg cacttactat gtgctaagca tggttctaag cactggagat 39960 atagcagtga ctaaggtgga taaaaatctg cccctggggc cagacgcgat ggctcacgcc 40020 tgtaatccca acacttttgg gaggtgggcg gatcacgagg tcaggagttc aagaccagcc 40080 tggccaacat ggtgaaaccc catctctact agaaatacaa aaattagcca ggcttggtgg 40140 cgtgcacctg taatcccaga tactagggtg gctgaggcag gagaattgct taaacctggg 40200 aggtgggggt tgcaatgagc cgagatcacg ccattgcgct ccagcctggc aacagaggga 40260 ggctccctct caaaacaaca acaacaacaaa caaaaatctc actcactgat 40335 tagtaacctt <210> 4 <211> 757 <212> PRT <213> Homo sapiens <400> 4 Met Ser Leu Leu Phe Ser Arg Cys Asn Ser Ile Val Thr Val Lys Lys 1 5 10 15 Asn Lys Arg His Met Ala Glu Val Asn Ala Ser Pro Leu Lys His Phe 20 25 30 Val Thr Ala Lys Lys Lys Ile Asn Gly Ile Phe Glu Gln Leu Gly Ala 35 40 45 Tyr Ile Gln Glu Ser Ala Thr Phe Leu Glu Asp Thr Tyr Arg Asn Ala 50 55 60 Glu Leu Asp Pro Val Thr Thr Glu Glu Gln Val Leu Asp Val Lys Gly 65 70 75 80 Tyr Leu Ser Lys Val Arg Gly Ile Ser Glu Val Leu Ala Arg Arg His 85 90 95 Met Lys Val Ala Phe Phe Gly Arg Thr Ser Asn Gly Lys Ser Thr Val 100 105 110 Ile Asn Ala Met Leu Trp Asp Lys Val Leu Pro Ser Gly Ile Gly His 115 120 125 Thr Thr Asn Cys Phe Leu Arg Val Glu Gly Thr Asp Gly His Glu Ala 130 135 140 Phe Leu Leu Thr Glu Gly Ser Glu Glu Lys Arg Ser Ala Lys Thr Val 145 150 155 160 Asn Gln Leu Ala His Ala Leu His Gln Asp Lys Gln Leu His Ala Gly 165 170 175 Ser Leu Val Ser Val Met Trp Pro Asn Ser Lys Cys Pro Leu Leu Lys 180 185 190 Asp Asp Leu Val Leu Met Asp Ser Pro Gly Ile Asp Val Thr Thr Glu 195 200 205 Leu Asp Ser Trp Ile Asp Lys Phe Cys Leu Asp Ala Asp Val Phe Val 210 215 220 Leu Val Ala Asn Ser Glu Ser Thr Leu Met Gln Thr Glu Lys His Phe 225 230 235 240 Phe His Lys Val Ser Glu Arg Leu Ser Arg Pro Asn Ile Phe Ile Leu 245 250 255 Asn Asn Arg Trp Asp Ala Ser Ala Ser Glu Pro Glu Tyr Met Glu Glu 260 265 270 Val Arg Arg Gln His Met Glu Arg Cys Thr Ser Phe Leu Val Asp Glu 275 280 285 Leu Gly Val Val Asp Arg Ser Gln Ala Gly Asp Arg Ile Phe Phe Val 290 295 300 Ser Ala Lys Glu Val Leu Asn Ala Arg Ile Gln Lys Ala Gln Gly Met 305 310 315 320 Pro Glu Gly Gly Gly Ala Leu Ala Glu Gly Phe Gln Val Arg Met Phe 325 330 335 Glu Phe Gln Asn Phe Glu Arg Arg Phe Glu Glu Cys Ile Ser Gln Ser 340 345 350 Ala Val Lys Thr Lys Phe Glu Gln His Thr Val Arg Ala Lys Gln Ile 355 360 365 Ala Glu Ala Val Arg Leu Ile Met Asp Ser Leu His Met Ala Ala Arg 370 375 380 Glu Gln Gln Val Tyr Cys Glu Glu Met Arg Glu Glu Arg Gln Asp Arg 385 390 395 400 Leu Lys Phe Ile Asp Lys Gln Leu Glu Leu Leu Ala Gln Asp Tyr Lys 405 410 415 Leu Arg Ile Lys Gln Ile Thr Glu Glu Val Glu Arg Gln Val Ser Thr 420 425 430 Ala Met Ala Glu Glu Ile Arg Arg Leu Ser Val Leu Val Asp Asp Tyr 435 440 445 Gln Met Asp Phe His Pro Ser Pro Val Val Leu Lys Val Tyr Lys Asn 450 455 460 Glu Leu His Arg His Ile Glu Glu Gly Leu Gly Arg Asn Met Ser Asp 465 470 475 480 Arg Cys Ser Thr Ala Ile Thr Asn Ser Leu Gln Thr Met Gln Gln Asp 485 490 495 Met Ile Asp Gly Leu Lys Pro Leu Leu Pro Val Ser Val Arg Ser Gln 500 505 510 Ile Asp Met Leu Val Pro Arg Gln Cys Phe Ser Leu Asn Tyr Asp Leu 515 520 525 Asn Cys Asp Lys Leu Cys Ala Asp Phe Gln Glu Asp Ile Glu Phe His 530 535 540 Phe Ser Leu Gly Trp Thr Met Leu Val Asn Arg Phe Leu Gly Pro Lys 545 550 555 560 Asn Ser Arg Arg Ala Leu Met Gly Tyr Asn Asp Gln Val Gln Arg Pro 565 570 575 Ile Pro Leu Thr Pro Ala Asn Pro Ser Met Pro Pro Leu Pro Gln Gly 580 585 590 Ser Leu Thr Gln Glu Glu Phe Met Val Ser Met Val Thr Gly Leu Ala 595 600 605 Ser Leu Thr Ser Arg Thr Ser Met Gly Ile Leu Val Val Gly Gly Val 610 615 620 Val Trp Lys Ala Val Gly Trp Arg Leu Ile Ala Leu Ser Phe Gly Leu 625 630 635 640 Tyr Gly Leu Leu Tyr Val Tyr Glu Arg Leu Thr Trp Thr Thr Lys Ala 645 650 655 Lys Glu Arg Ala Phe Lys Arg Gln Phe Val Glu His Ala Ser Glu Lys 660 665 670 Leu Gln Leu Val Ile Ser Tyr Thr Gly Ser Asn Cys Ser His Gln Val 675 680 685 Gln Gln Glu Leu Ser Gly Thr Phe Ala His Leu Cys Gln Gln Val Asp 690 695 700 Val Thr Arg Glu Asn Leu Glu Gln Glu Ile Ala Ala Met Asn Lys Lys 705 710 715 720 Ile Glu Val Leu Asp Ser Leu Gln Ser Lys Ala Lys Leu Leu Arg Asn 725 730 735 Lys Ala Gly Trp Leu Asp Ser Glu Leu Asn Met Phe Thr His Gln Tyr 740 745 750 Leu Gln Pro Ser Arg 755

Claims (20)

NFX1 gene or protein encoded by it; and the MFN2 gene or a protein encoded by it; A composition for predicting cancer prognosis, comprising an agent for measuring the expression level of at least one of. The method of claim 1,
The cancer prognosis is the survival rate or survival period of cancer patients, or the prognosis after anticancer drug treatment, the composition for predicting cancer prognosis. 3. The method of claim 2,
The anticancer agent is an OXPHOS inhibitor, irinotecan or a combination thereof, a composition for predicting cancer prognosis. 4. The method of claim 3,
The OXPHOS inhibitor is a ubiquinone reducing activity inhibitor, a mitochondrial electron transport chain I inhibitor, and a composition for predicting cancer prognosis comprising at least one selected from the group consisting of an iron chelator. 5. The method of claim 4,
The ubiquinone reducing activity inhibitor is IACS-010759,
The mitochondrial electron transport chain I inhibitor inhibitor is at least one selected from the group consisting of IM156, metformin and rotenone,
The iron chelator is VLX 600, a composition for predicting cancer prognosis. The method of claim 1,
The agent for measuring the expression level of the protein comprises at least one selected from the group consisting of an antibody, oligopeptide, ligand, PNA (peptide nucleic acid) and aptamer that specifically binds to the protein, cancer A composition for predicting prognosis. The method of claim 1,
The agent for measuring the expression level of the gene comprises at least one selected from the group consisting of primers, probes and antisense nucleotides that specifically bind to the gene, a composition for predicting cancer prognosis. The method of claim 1,
The cancer is breast cancer, uterine cancer, esophageal cancer, stomach cancer, brain cancer, rectal cancer, colorectal cancer, lung cancer, skin cancer, ovarian cancer, cervical cancer, kidney cancer, blood cancer, pancreatic cancer, prostate cancer, testicular cancer, laryngeal cancer, oral cancer, head and neck cancer, thyroid cancer, Liver cancer, bladder cancer, osteosarcoma, lymphoma, leukemia, thymus cancer, squamous cell carcinoma, adenocarcinoma, and a composition for predicting cancer prognosis selected from the group consisting of a combination thereof. A kit for predicting cancer prognosis comprising the composition of any one of claims 1 to 8. 10. The method of claim 9,
The kit is an RT-PCR kit, a DNA chip kit, an ELISA kit, a protein chip kit, a rapid kit or a multiple reaction monitoring (MRM) kit, a kit for predicting cancer prognosis. NFX1 gene or a protein encoded by it in a biological sample isolated from a subject of interest; and the MFN2 gene or a protein encoded by it; A method for providing information for predicting cancer prognosis, comprising measuring the expression level of at least one of. 12. The method of claim 11,
When the measured NFX1 gene or the protein expression level encoded by it increases compared to the control group, predicting that the target individual has a good prognosis after anticancer drug treatment, a method for predicting cancer prognosis. 13. The method of claim 12,
The anticancer agent is an OXPHOS inhibitor, irinotecan, or a combination thereof, an information providing method for predicting cancer prognosis. 12. The method of claim 11,
When the measured expression level of the MFN2 gene or the protein encoded by it is increased compared to the control, the target individual is predicted to have a low survival rate or a short survival period, a method for predicting cancer prognosis. 12. The method of claim 11,
The cancer is breast cancer, uterine cancer, esophageal cancer, stomach cancer, brain cancer, rectal cancer, colorectal cancer, lung cancer, skin cancer, ovarian cancer, cervical cancer, kidney cancer, blood cancer, pancreatic cancer, prostate cancer, testicular cancer, laryngeal cancer, oral cancer, head and neck cancer, thyroid cancer, An information providing method for predicting cancer prognosis, selected from the group consisting of liver cancer, bladder cancer, osteosarcoma, lymphoma, leukemia, thymus cancer, squamous cell carcinoma, adenocarcinoma, and combinations thereof. a sample unit containing a biological sample obtained from a subject of interest; and
NFX1 gene or a protein encoded by it in the biological sample; and the MFN2 gene or protein encoded by it; A device for predicting a cancer prognosis, comprising a; detection unit for detecting the expression level of at least one of. 17. The method of claim 16,
The device further comprises a computing unit for predicting a cancer prognosis by comparing the expression level obtained from the detection unit with the expression level in the control device, for predicting cancer prognosis. 18. The method of claim 17,
In the calculation unit, when the expression level of the NFX1 gene or the protein encoded by the detection unit is increased compared to the control unit, predicting that the prognosis of the target subject after anticancer drug treatment is good, a device for predicting a cancer prognosis. 19. The method of claim 18,
The anticancer agent is an OXPHOS inhibitor, irinotecan, or a combination thereof, a device for predicting a cancer prognosis. 19. The method of claim 18,
In the calculation unit, when the expression level of the MFN2 gene or the protein encoded by the detection unit increases compared to the control unit, the prognosis of the target individual is predicted to be poor, an apparatus for predicting a cancer prognosis.

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